CN113016826A

CN113016826A - Preparation process of multifunctional microbial organic seed coating agent

Info

Publication number: CN113016826A
Application number: CN202110416042.3A
Authority: CN
Inventors: 李久庆; 屈庆涛; 屈凡河; 赵越
Original assignee: Beijing Zhongnongfuyuan Group Co ltd
Current assignee: Beijing Zhongnongfuyuan Group Co ltd
Priority date: 2021-04-19
Filing date: 2021-04-19
Publication date: 2021-06-25
Anticipated expiration: 2041-04-19
Also published as: CN113016826B

Abstract

The application relates to the field of seed coating technology, and particularly discloses a preparation process of a multifunctional microbial organic seed coating. The preparation process of the multifunctional microbial organic seed coating agent comprises the following preparation steps: uniformly mixing 8-15 parts of A-type bacterium powder, 10-20 parts of B-type bacterium powder, 5-18 parts of yeast powder, 3-5 parts of protein powder, 5-10 parts of corn steep liquor dry powder and 5-10 parts of starch in parts by weight to obtain a multifunctional microbial organic seed coating agent; the A-type bacterium powder is prepared from bacillus strains, and the B-type bacterium powder is prepared from bacteriocidal strains. The preparation process can prepare the multifunctional microbial organic seed coating agent with insecticidal and disease-resistant effects; in addition, the multifunctional microbial organic seed coating agent prepared by the preparation process has the advantages of environmental friendliness and no pollution, and is beneficial to reducing the pollution of the seed coating agent to the environment and crop fruits.

Description

Preparation process of multifunctional microbial organic seed coating agent

Technical Field

The application relates to the field of seed coating technology, in particular to a preparation process of a multifunctional microbial organic seed coating.

Background

At present, most seed coating agents at home and abroad take pesticides or chemical fertilizers as raw materials, and are coated or bonded with target seeds after chemical treatment so as to achieve the purpose of coating. After the seeds are treated by the seed coating agent, partial insect pests and diseases can be prevented and treated.

In the related technology, difenoconazole and imidacloprid are used as effective raw materials of a suspension seed coating agent, and the difenoconazole is a heterocyclic bactericide pesticide with toxicity, has small mobility in soil and is slowly degraded; imidacloprid is a toxic nitro methylene systemic insecticide, and has a residual period of up to 25 days.

In view of the above-mentioned related technologies, the inventors believe that the above-mentioned suspension seed coating agent causes soil and groundwater pollution, and at the same time, causes pesticide residue on the crop fruits.

Disclosure of Invention

In order to reduce the pollution of the seed coating agent to the environment and crop fruits, the application provides a preparation process of the multifunctional microbial organic seed coating agent.

The preparation process of the multifunctional microbial organic seed coating agent provided by the application adopts the following technical scheme:

a preparation process of a multifunctional microbial organic seed coating agent comprises the following preparation steps:

uniformly mixing 8-15 parts of A-type bacterium powder, 10-20 parts of B-type bacterium powder, 5-18 parts of yeast powder, 3-5 parts of protein powder, 5-10 parts of corn steep liquor dry powder and 5-10 parts of starch in parts by weight to obtain a multifunctional microbial organic seed coating agent; the A-type bacterium powder is prepared from bacillus strains, and the B-type bacterium powder is prepared from bacteriocidal strains.

By adopting the technical scheme, as the fungus powder and the absorbable and degradable raw materials made of different types of microorganism strains are adopted, the pollution of the seed coating agent to the environment and the crop fruits is effectively reduced, the sterilization strains can effectively prevent and control the root-knot nematode and other conventional underground pests, and the bacillus strains can effectively prevent the soil-borne diseases, bacteria and fungal diseases of the crops; the bacillus strains are prepared into the A-type strain powder, and the sterilization strains are prepared into the B-type strain powder, so that the activity of effective bacteria is enhanced, the seed coating agent can play insecticidal and disease-resistant effects for a long time, and the germination and growth of seeds are effectively assisted; therefore, the preparation process can prepare the multifunctional microbial organic seed coating agent with green, pollution-free, insecticidal and disease-resistant effects, is beneficial to reducing the pollution of the seed coating agent to the environment and crop fruits, and is simple, convenient and easy to prepare.

Preferably, the A-type bacterium powder is prepared by the following steps:

inoculating the bacillus strains in a shake flask, culturing for 4-10h, and inoculating the bacillus strains in a sterilized A-type fermentation tank;

fermenting the A-type fermentation tank for 2-3 days under the conditions of 120-240r/min and 32-38 ℃ to obtain the bacillus strain fermentation liquor with the strain sporulation rate of more than 99%;

separating the bacillus strain fermentation liquor to obtain clear liquid and a heavy phase, and carrying out spray drying on the heavy phase to obtain A-type bacteria powder with the water content of 28-32% and the bacteria content of more than 500 hundred million/g.

By adopting the technical scheme, the shake flask is inoculated and cultured in advance, and then the strain is inoculated to the type A fermentation tank, so that the pollution of mixed bacteria to the type A fermentation tank is reduced, the pollution of bacillus strain fermentation liquor by the mixed bacteria is reduced, the spore yield of the strain is cultured to be more than 99%, the content of effective bacteria in the bacillus strain fermentation liquor is improved, and the type A bacteria powder is prepared by spray drying, so that the activity of the effective bacteria in the type A bacteria powder is improved.

Preferably, the bacillus strain comprises bacillus subtilis and paenibacillus polymyxa, and the paenibacillus A powder comprises 3-5 parts by weight of bacillus subtilis powder and 5-10 parts by weight of paenibacillus polymyxa powder.

By adopting the technical scheme, the bacillus subtilis can generate active substances such as subtilin, polymyxin, nystatin, gramicidin and the like, and the active substances have obvious inhibiting effect on pathogenic bacteria or pathogenic bacteria with endogenous infection; the bacillus polymyxa has strong colonization ability, and pathogenic bacteria can be prevented from infecting plants through site competition; meanwhile, broad-spectrum antibacterial substances continuously secreted by the bacillus polymyxa can inhibit or kill pathogenic bacteria, so that the bacillus subtilis and the paenibacillus polymyxa are beneficial to preventing soil-borne diseases, bacterial diseases and fungal diseases of crops.

Preferably, the separation and the spray drying of the fermentation liquor of the bacillus strain are both carried out in a centrifugal drying integrated machine, the centrifugal drying all-in-one machine comprises a centrifugal machine body, a drying machine body and a rotating assembly, wherein the drying machine body comprises a drying cylinder, a centrifugal atomizer, a spray head and an air injection piece, the centrifuge body is communicated with the centrifugal atomizer, the lower end of the centrifuge body is provided with a sealing cover, the centrifugal atomizer is arranged on the sealing cover, the spray head is arranged in the sealing cover and is communicated with the centrifugal atomizer, at least two drying cylinders are arranged, the drying cylinders are connected with the rotating assembly, one of the drying cylinders is a working cylinder, the other drying cylinders are preparatory cylinders, the top end of the working cylinder is abutted against the bottom end of the sealing cover, the spray head faces the inside of the drying cylinder, and the air injection piece is installed on the drying cylinder;

the rotary component comprises a base, a rotary pipe, a central shaft, a connecting rod and a rotary motor, wherein the central shaft is fixedly connected to the base, the rotary pipe is sleeved on the central shaft, the connecting rod is fixedly connected to the peripheral wall of the rotary pipe, one end of the rotary pipe, far away from the connecting rod, is fixedly connected with a rotary gear, the rotary motor is installed on the base, a driving gear is installed on the rotary motor, and the driving gear is meshed with the rotary gear.

By adopting the technical scheme, the centrifugal atomizer is used for atomizing the heavy phase, and the spray head sprays the heavy phase in a mist form, so that the heavy phase is dried in a short time, and the activity of effective bacteria in the heavy phase is improved; the rotating assembly can drive the drying cylinders to rotate, so that the plurality of drying cylinders can work or clean in turn, and the working efficiency is improved; the base and the central shaft can enhance the stability of the rotary pipe and help to reduce the shaking of the drying cylinder during rotation, and the rotary motor, the driving gear and the rotary gear are matched to drive the rotary pipe to rotate, so that the drying cylinder can rotate; therefore, the centrifugal drying all-in-one machine is beneficial to reducing pollution of A-type bacterium powder and keeping the activity of the strain, and is simple and quick to operate and beneficial to improving the separation and drying efficiency.

Preferably, fixedly connected with thrust cylinder on the gland, thrust cylinder's flexible end fixedly connected with sealing ring, the sealing ring cover is established on the gland, fixedly connected with seal ring on the internal perisporium of sealing ring, the periphery wall of sealed lid and drying cylinder all with seal ring's internal perisporium butt.

Through adopting above-mentioned technical scheme, sealing ring and seal ring can seal the gap between sealed lid and the dryer tube, reduce gaseous leakage from the gap, and simultaneously, the thrust cylinder is used for driving sealing ring and seal ring to remove for the sealing ring does not influence the dryer tube and rotates.

Preferably, the centrifuge body comprises a base, a rotary drum, a telescopic piston, a liquid discharge pipe and a slag discharge pipe, the rotary drum is rotatably mounted on the base, a centrifugal motor for driving the rotary drum is arranged on the base, the slag discharge pipe comprises a first slag discharge pipe, a second slag discharge pipe and a threaded ring, the first slag discharge pipe is communicated with the bottom end of the rotary drum, the threaded ring is in threaded connection with the bottom end of the first slag discharge pipe, the top end of the second slag discharge pipe is in threaded connection with one end, far away from the first slag discharge pipe, of the threaded ring, the other end of the second slag discharge pipe is fixedly connected with a sealing cover, the spray head and the centrifugal atomizer are both communicated with the second slag discharge pipe, and control valves are mounted on the liquid discharge pipe and; the telescopic piston comprises a power motor, a piston head, a toothed bar, a piston rod and a power gear, the power motor is fixedly mounted on the base, the power gear is mounted at the output end of the power motor, the toothed bar is connected to the base in a sliding mode along the vertical direction, the power gear is meshed with the toothed bar, the top end of the piston rod is connected with the toothed bar in a rotating mode, the bottom end of the piston rod is fixedly connected with the piston head, the piston head is abutted to the inner circumferential wall of the rotary drum in a sliding mode, a scraper is connected to the lower surface of the piston head in a rotating mode, and a driving motor used for driving the scraper.

Through adopting above-mentioned technical scheme, centrifugal motor drive rotary drum rotates, can separate into clear solution and heavy phase with bacillus class bacterial strain zymotic fluid, heavy phase deposit is on the inner wall of rotary drum, the fluid-discharge tube can be with clear solution discharge rotary drum, telescopic piston is used for pushing heavy phase to in arranging the sediment pipe, driving motor drive doctor-bar rotates, help scraping the heavy phase that glues on centrifuge body inner wall, arrange sediment one pipe, arrange sediment two pipe and screw ring and help reducing the hindrance of sediment pipe to rotary drum rotation, telescopic piston and row's sediment pipe cooperation, can directly carry the heavy phase to dry centrifugal atomizer in through atmospheric pressure, need not open centrifuge body manual sediment of getting, convenient and fast.

Preferably, the B-type bacterium powder is prepared by the following steps:

culturing the bactericidal strains until spore is produced, and inoculating the bactericidal strains after spore production into a B-type fermentation tank after sterilization; fermenting the B-type fermentation tank for 3-5 days under the conditions of 120-;

preparing the bacterial strain fermentation liquor into bacterial cakes, and carrying out flash evaporation drying on the bacterial cakes to obtain B-type bacterial powder with the water content of 28-32% and the bacterial content of spore powder of more than 100 hundred million/g.

By adopting the technical scheme, the bactericidal strains are cultured to produce spores, and then fermentation is carried out, so that the survival of the bactericidal strains and the shortening of the fermentation time are facilitated, and under the conditions of the water content and the bacteria content, the activity of the bactericidal strains in the B-type bacterium powder is stronger, and the bactericidal effect is better.

Preferably, the sterilization bacterial strain comprises lilium purple spore powder and metarhizium anisopliae, and the B bacterial powder comprises 5-10 parts by weight of lilium purple spore powder and 5-10 parts by weight of metarhizium anisopliae spore powder.

By adopting the technical scheme, the lilac purple spore fungus can be parasitized in ova, larvae and mother worms, and the metarhizium anisopliae can invade the insect bodies and generate insecticidal toxin, so that the pest damage of crops can be reduced.

Preferably, 1-2 parts by weight of an antifoaming agent is added to the type A fermentor or the type B fermentor 0-12h before the end of the fermentation process in the type A fermentor or the type B fermentor.

By adopting the technical scheme, the defoaming agent is added, so that the foam amount of the fermentation liquor in the later fermentation period is reduced, and the death of the bacterial strains in the fermentation liquor is reduced.

Preferably, the feed also comprises 5-10 parts by weight of amino acid powder, 10-15 parts by weight of active enzyme and 5-10 parts by weight of nutrient containing trace elements.

By adopting the technical scheme, the germination vigor and the germination rate can be improved by the active enzyme, the germination of seeds is facilitated, amino acid and trace elements required by the growth of the seeds can be provided by the amino acid powder and the nutrient containing the trace elements, the chlorophyll content, the thousand seed weight, the root length, the leaf thickness and the like of plants are facilitated to be improved, and the germination and the growth of the seeds are facilitated; in addition, the amino acid powder, the active enzyme and the nutrient containing the trace elements can be degraded, so that the pollution to the environment and the crop fruits can be effectively reduced.

In summary, the present application has the following beneficial effects:

1. the bacterial powder prepared from different types of microbial strains and absorbable and degradable raw materials is beneficial to reducing the pollution of the seed coating agent to the environment and crop fruits, and the bacterial powder prepared from the strains is beneficial to enhancing the insecticidal and disease-resistant effects of the seed coating agent.

2. The utility model provides a centrifugal drying all-in-one, through flexible piston and scum pipe, can be directly carry the heavy phase to this internal, convenient and fast, dehumidification case, sterilization case and accuse temperature case can be with air current control at suitable temperature, detach miscellaneous fungus and pollutant in the air current, help keeping the activity of bacterial strain in the A class fungus powder, rotating assembly can drive the rotation of drying cylinder, make a plurality of drying cylinders can work or clean in turn, help improving work efficiency.

3. The utility model provides a rotating assembly includes base, swinging head, center pin, connecting rod and rotation motor, can carry out the drying operation at a dryer, and the clearance has just used the dryer, piles up impurity in the reduction dryer, helps reducing the coating agent and receives the pollution.

Drawings

Fig. 1 is a schematic structural diagram of a centrifugal drying all-in-one machine in embodiment 1 of the present application.

Fig. 2 is a plan view of the centrifugal drying all-in-one machine according to embodiment 1 of the present application.

Fig. 3 is a sectional view taken along line a-a of fig. 2.

Fig. 4 is a view from direction B in fig. 3.

Fig. 5 is a view in the direction of C in fig. 3.

Fig. 6 is a schematic structural view of a rotating assembly according to embodiment 1 of the present application.

Fig. 7 is a sectional view of a sterilization case structure embodying embodiment 1 of the present application.

Fig. 8 is a sectional view of a temperature control box structure for embodying embodiment 1 of the present application.

Description of reference numerals: 1. a centrifuge body; 11. a machine base; 111. a platform; 1111. a motor slot; 112. a housing; 12. a rotating drum; 13. a telescopic piston; 131. a power motor; 132. a piston head; 133. a rack bar; 134. a piston rod; 135. a power gear; 14. a liquid discharge pipe; 15. a slag discharge pipe; 151. discharging slag in a first pipe; 152. a second slag discharge pipe; 153. a threaded ring; 16. a centrifugal motor; 17. scraping a blade; 18. a drive motor; 19. a sealing cover; 191. a thrust cylinder; 192. a seal ring; 193. a sealing gasket; 194. an exhaust pipe; 1941. a safety valve; 2. a dryer body; 21. a drying cylinder; 211. an opening; 22. a centrifugal atomizer; 23. a spray head; 24. an air-jet member; 241. a gas ejector tube; 2411. a first tube; 2412. a second tube; 2413. a third tube; 242. a dehumidification box; 243. a sterilization box; 2431. sealing the box body; 2432. an ultraviolet germicidal lamp; 2433. a heating lamp; 2434. a baffle; 244. a temperature control box; 2441. a warm box body; 2442. winding the tube; 2443. a temperature sensor; 3. a rotating assembly; 31. a base; 32. rotating the tube; 321. a rotating gear; 33. a central shaft; 34. a connecting rod; 35. rotating the motor; 351. a driving gear; 4. and (4) controlling the valve.

Detailed Description

The starting materials in the examples of the present application are all commercially available. Wherein, the bacillus subtilis is purchased from Jinnan Okai chemical industry Co., Ltd, the Paenibacillus polymyxa is purchased from Weifang Rui Bio-technology Co., Ltd, the lilac purple spore is purchased from Guangzhou genuine and fake bio-technology Co., Ltd, the metarhizium anisopliae is purchased from Shanghai Hu Zheng Bio-technology Co., Ltd, the yeast powder is purchased from Shandong Wangbao bio-technology Co., Ltd, the protein powder is pea protein powder purchased from Suzhou Meiyi Hu Bio-technology Co., Ltd, the corn steep liquor dry powder is purchased from Jinnan Qingyu Yu Co., Ltd, the starch is purchased from Nanjing Cheng Yu Bio-technology Co., Ltd, the amino acid powder is purchased from Jinan Sheng chemical industry Co., Ltd, the active enzyme is D-lactate dehydrogenase purchased from West Anqiyue Bio-technology Co., the New Material, the nutrient containing trace elements is agricultural zinc sulfate material purchased from Nanjing Jinju Jinjui Yu Co., Ltd, and the defoaming agent is a food grade new.

The present application is described in further detail below with reference to figures 1-8 and examples.

Examples

Examples 1 to 3

As shown in Table one, the main difference between examples 1-3 is the amount of starting material used.

The following description will be given by taking example 1 as an example.

The embodiment provides a centrifugal drying all-in-one machine, and referring to fig. 1, the centrifugal drying all-in-one machine comprises a centrifuge body 1, a dryer body 2 and a rotating assembly 3.

Referring to fig. 2 and 3, the centrifuge body 1 includes a base 11, a drum 12, a telescopic piston 13, a liquid discharge pipe 14 and a slag discharge pipe 15, the base 11 includes a platform 111 and a housing 112, the bottom end of the drum 12 is rotatably connected to the platform 111, the housing 112 is sleeved on the outer side of the drum 12, and the housing 112 is welded to the platform 111.

Referring to fig. 3 and 4, a motor groove 1111 is arranged in the platform 111, a centrifugal motor 16 is riveted on the groove wall of the motor groove 1111, and an output shaft of the centrifugal motor 16 is welded with the bottom wall of the rotary drum 12; the telescopic piston 13 comprises a power motor 131, a piston head 132, a toothed bar 133, a piston rod 134 and a power gear 135, the power motor 131 is riveted on the top wall of the shell 112, the power gear 135 is installed on an output shaft of the power motor 131, a sliding groove is formed in the top wall of the shell 112, the toothed bar 133 is in sliding butt joint with the groove wall of the sliding groove, the power gear 135 is meshed with the toothed bar 133, the top end of the piston rod 134 is rotatably connected with the bottom end of the toothed bar 133, the bottom end of the piston rod 134 is integrally connected with the piston head 132, the piston rod 134 and the piston head 132 are both inserted into the rotary drum 12, the piston rod 134 is rotatably connected with the rotary drum 12, the peripheral wall of the piston head 132 is in butt joint.

Referring to FIGS. 3 and 4, drain 14 is welded to the top wall of bowl 12, with the bottom end of drain 14 extending into bowl 12, and with drain 14 extending through piston head 132 and slidably engaging piston head 132; the slag discharge pipe 15 is welded on the bottom wall of the rotary drum 12, the control valve 4 is installed on the liquid discharge pipe 14 and the slag discharge pipe 15, the control valve 4 is an electromagnetic valve, the top end of the slag discharge pipe 15 penetrates through the bottom wall of the rotary drum 12, and the bottom end of the slag discharge pipe 15 is connected with the dryer body 2.

Referring to fig. 4, the driving motor 18 is installed inside the piston head 132, an output shaft of the driving motor 18 penetrates through a bottom wall of the piston head 132, the wiper 17 is welded to the output shaft of the driving motor 18, and the wiper 17 abuts against the bottom wall of the piston head 132.

Referring to fig. 3 and 4, the dryer body 2 includes a drying cylinder 21, a centrifugal atomizer 22, a spray head 23, and a jet member 24; the slag discharge pipe 15 comprises a first slag discharge pipe 151, a second slag discharge pipe 152 and a threaded ring 153, the top end of the first slag discharge pipe 151 is welded with the bottom wall of the rotary drum 12 and communicated with the rotary drum 12, the bottom end of the first slag discharge pipe 151 is in threaded connection with the threaded ring 153, the top end of the second slag discharge pipe 152 is in threaded connection with the threaded ring 153, the bottom end of the second slag discharge pipe 152 is fixedly connected with the centrifugal atomizer 22, the control valve 4 is installed on the first slag discharge pipe 151, a hand hole is formed in the outer surface of the platform 111 and communicated with the motor groove 1111, and the spray head 23 is installed at the bottom end of the second slag discharge pipe.

Referring to fig. 4 and 5, the slag discharge secondary pipe 152 is welded with a sealing cover 19, the nozzle 23 is arranged in the sealing cover 19, and the nozzle 23 is in threaded connection with the slag discharge secondary pipe 152; the upper surface of drying cylinder 21 is equipped with opening 211, and drying cylinder 21 includes a working cylinder and two and prepares a section of thick bamboo, and the working cylinder links together through rotating component 3 with two, and when the working cylinder moved to sealed lid 19 below, the upper surface of working cylinder and the lower terminal wall butt of sealed lid 19, simultaneously, shower nozzle 23 was towards opening 211.

Referring to fig. 5 and 6, the air injection member 24 is installed on the drying cylinder 21, and the air injection member 24 injects air into the drying cylinder 21; the gas spraying piece 24 comprises a gas spraying pipe 241, a dehumidifying box 242, a sterilizing box 243 and a temperature control box 244, wherein the gas spraying pipe 241 is welded on the drying cylinder 21, and the dehumidifying box 242, the sterilizing box 243 and the temperature control box 244 are sequentially welded on the outer wall of the gas spraying pipe 241; the air injection pipe 241 comprises a first pipe 2411, a second pipe 2412 and a third pipe 2413, the first pipe 2411 is welded on the dehumidification box 242, one end of the second pipe 2412 is welded on the side wall of the dehumidification box 242 far away from the first pipe 2411, the other end of the second pipe 2412 is welded on the sterilization box 243, one end of the third pipe 2413 is welded on the side wall of the sterilization box 243 far away from the second pipe 2412, the other end of the third pipe 2413 is welded on the drying cylinder 21, the third pipe 2413 penetrates through the drying cylinder 21, and the air outlet end of the third pipe 2413 is positioned below the spray head 23.

Referring to fig. 5 and 6, an exhaust pipe 194 is fixedly connected to the top wall of the sealing cover 19, a safety valve 1941 is installed on the exhaust pipe 194, a thrust cylinder 191 is riveted to the outer wall of the sealing cover 19, the telescopic end of the thrust cylinder 191 faces the bottom end of the sealing cover 19, a sealing ring 192 is welded to the telescopic end of the thrust cylinder 191, the sealing ring 192 is sleeved on the sealing cover 19, a sealing washer 193 is glued to the inner wall of the sealing ring 192, and the sealing washer 193 is in sliding abutting contact with the outer peripheral wall of the sealing cover.

Referring to fig. 5 and 6, the rotating assembly 3 includes a base 31, a rotating pipe 32, a central shaft 33, a connecting rod 34 and a rotating motor 35, wherein the bottom end of the central shaft 33 is welded on the base 31, the rotating pipe 32 is sleeved on the central shaft 33, and the inner peripheral wall of the rotating pipe 32 is abutted to the central shaft 33; one end of the connecting rod 34 is welded on the rotating pipe 32, and the other end of the connecting rod 34 is welded on the outer wall of the drying cylinder 21; a rotating gear 321 is arranged on the outer wall of the rotating pipe 32, a through hole is formed in the rotating gear 321, the rotating pipe 32 penetrates through the through hole, and the rotating pipe 32 is welded with the rotating gear 321; the outer wall of the rotating motor 35 is riveted on the base 31, a driving gear 351 is welded on an output shaft of the rotating motor 35, and the driving gear 351 is welded with the rotating gear 321.

Referring to fig. 7, the sterilization chamber 243 includes a sealed chamber 2431, an ultraviolet germicidal lamp 2432, a heating lamp 2433, and guide plates 2434, wherein the second tube 2412 and the third tube 2413 are welded to the sealed chamber 2431, three guide plates 2434 are welded to an inner wall of the sealed chamber 2431, five guide plates 2434 are arranged to be staggered up and down, and the ultraviolet germicidal lamp 2432 and the heating lamp 2433 are installed on the inner wall of the sealed chamber 2431.

Referring to fig. 8, the temperature control box 244 includes a heat preservation box 2441, a winding pipe 2442 and a temperature sensor 2443, the third pipe 2413 is welded to the heat preservation box 2441, the third pipe 2413 penetrates through the heat preservation box 2441, the winding pipe 2442 is wound on the third pipe 2413, the winding pipe 2442 is an aluminum alloy hose, two ends of the winding pipe 2442 extend out of the heat preservation box 2441, the temperature sensor 2443 is installed on a pipe wall of the third pipe 2413, and the temperature sensor 2443 is a thermometer.

The working principle of the centrifugal drying all-in-one machine provided by the embodiment is as follows: conveying bacillus strain fermentation liquor into the rotary drum 12 from the liquid discharge pipe 14, extending hands into the motor groove 1111 from hand holes, rotating the threaded ring 153 to separate the first slag discharge pipe 151 from the second slag discharge pipe 152, starting the centrifugal motor 16, driving the rotary drum 12 to rotate by the centrifugal motor 16, centrifugally separating the bacillus strain fermentation liquor into clear liquid and heavy phases, then opening the control valve 4 on the liquid discharge pipe 14, starting the telescopic cylinder 131, pushing the piston head 132 to move downwards, scraping the heavy phases attached to the inner wall of the rotary drum 12 by the piston head 132, simultaneously discharging the clear liquid from the liquid discharge pipe 14 under the action of air pressure, and then closing the control valve 4 on the liquid discharge pipe 14; then, the control valve 4 on the first slag discharge pipe 151 is opened, the piston head 132 continues to move downwards, heavy phases are conveyed into the centrifugal atomizer 22 from the first slag discharge pipe 151 and the second slag discharge pipe 152 under the action of air pressure, when the piston head 132 moves to the bottom end of the rotary drum 12, the telescopic air cylinder 131 is closed, the driving motor 18 is started, the driving motor 18 drives the scraping blade 17 to rotate, the scraping blade 17 scrapes the heavy phases on the inner bottom wall of the rotary drum 12, and the scraped heavy phases are conveyed into the centrifugal atomizer 22 from the first slag discharge pipe 151 and the second slag discharge pipe 152.

The rotating motor 35 is started, the rotating motor 35 drives the driving gear 351 to rotate, the rotating gear 321, the rotating pipe 32, the connecting rod 34 and the drying cylinders 21 synchronously rotate, when one drying cylinder 21 moves to the position below the sealing cover 19, the rotating motor 35 is closed, the thrust cylinder 191 is started again, the thrust cylinder 191 pushes the sealing ring 192 to move, and when the sealing ring 192 and the sealing gasket 193 close the gap between the drying cylinder 21 and the sealing cover 19, the thrust cylinder 191 is closed.

The centrifugal atomizer 22 disperses the heavy phase into a mist state, and then the mist state is sprayed into the drying cylinder 21 from the spray head 23, meanwhile, hot air flows enter the dehumidifying box 242 along the first pipe 2411, the dehumidified hot air flows enter the sealed box 2431 along the second pipe 2412, the hot air flows travel along the gap between the guide plates 2434 according to an S-shaped route, the ultraviolet sterilizing lamp emits ultraviolet rays, the heating lamp 2433 heats the hot air flows, the sterilized hot air flows enter the heat preservation box 2441 along the third pipe 2413, the temperature of the hot air flows is sensed by the temperature sensor 2443 and data is transmitted to the control system, the control system controls water with different temperatures to enter the winding pipe 2442, the winding pipe 2442 adjusts the temperature of the third pipe 2413 to be a proper temperature, the temperature of the hot air flows in the third pipe 2413 is adjusted to be a proper range, and then the hot air flows are sprayed into the drying cylinder 21.

The hot air flow instantly dries the fog-state heavy phase sprayed by the spray head 23 into A-type bacteria powder, and the A-type bacteria powder falls to the bottom end of the drying cylinder 21 and is conveyed to the next process.

A multifunctional microbial organic seed coating agent is prepared by the following steps:

s1, after inoculating the bacillus strain in a shake flask, detecting OD600 every 2h, drawing a growth curve, and when the growth curve reaches the late logarithmic phase, inoculating the bacillus strain in a sterilized A-type fermentation tank, wherein the sterilization temperature of the A-type fermentation tank is 117 ℃, the sterilization time is 40 minutes, and the sterilization pressure is 0.15 MPa;

s2, inoculating the sterilization bacterial strain on a PDA plate culture medium, after the bacterial strain is cultured to produce spores, washing and flushing the sterilization bacterial strain by using sterile deionized water, and then inoculating the sterilization bacterial strain to a sterilized B-type fermentation tank, wherein the sterilization temperature of the B-type fermentation tank is 117 ℃, the sterilization time is 40 minutes, and the sterilization pressure is 0.15 MPa;

s3, fermenting the fermentation tank A for 2.5 days at the temperature of 35 ℃ and at the speed of 180r/min to obtain the bacillus strain fermentation liquor with the strain sporulation rate of more than 99%;

s4, fermenting the B-type fermentation tank for 4 days at 180r/min and 30 ℃ to obtain a fungicide strain fermentation liquor with a strain sporulation rate of more than 95%;

s5, adding the bacillus strain fermentation liquor into a centrifugal drying integrated machine, separating to obtain clear liquid and a heavy phase, and carrying out spray drying on the heavy phase until the water content is 30% to obtain A-type bacterium powder with the bacterium content of more than 500 hundred million/g;

s6, adding the bacterial strain fermentation liquor into a plate-and-frame filter press to prepare a bacterial cake, conveying the bacterial cake into a flash tower, and performing flash drying until the water content is 30% to obtain B-type bacterial powder with the bacterial content of spore powder being more than 100 hundred million/g;

s7, adding A-type bacteria powder, B-type bacteria powder, yeast powder, protein powder, corn steep liquor dry powder and starch into a mixing tank according to the table I, stirring for 15min at 160r/min to obtain a multifunctional microbial organic seed coating agent, conveying the multifunctional microbial organic seed coating agent into a filling machine, and bagging and packaging;

the bacillus strains comprise bacillus subtilis and paenibacillus polymyxa, and the A-type bacteria powder comprises bacillus subtilis powder and paenibacillus polymyxa powder; the bactericidal strains comprise lilium purple spore bacteria and metarhizium anisopliae, and the B-type bacteria powder comprises lilium purple spore powder and metarhizium anisopliae spore powder; the culture medium components of the A-type fermentation tank and the B-type fermentation tank are soybean meal, peanut cake powder, corn flour, fish meal, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, peptone, yeast powder, beef extract, glucose, zinc sulfate, growth factors and vitamins.

Table-raw material dosage table

Example 4

This example differs from example 3 in that in step S7, 7.5kg of amino acid powder, 12.5kg of active enzyme, and 7.5kg of trace element-containing nutrient were further added.

Example 5

This example differs from example 3 in that the moisture content of both group a and group B fungal powders was 28%.

Example 6

This example differs from example 3 in that the moisture content of both group a and group B fungal powders was 32%.

Example 7

This example differs from example 3 in that 1.5kg of antifoam was added to both the type A fermentor and the type B fermentor in the S3 and S4 steps within 6 hours before the end of fermentation.

Comparative example

Comparative example 1

The comparative example provides a composite biological seed coating agent, 30 percent of bacillus subtilis fermentation concentrated solution (the viable count is more than or equal to 1011cfu/g) and 15 percent of streptomyces rochei fermentation concentrated solution (the biomass is more than or equal to 70PMV/g) are added into a reaction kettle according to the synergistic combination proportion of the bacillus subtilis and the streptomyces rochei 2:1, and the mixture is fully stirred and mixed.

Adding 3.5% of sodium carboxymethylcellulose, 0.5% of sodium lignosulfonate, 1% of chitosan, 0.2% of sodium benzoate and 0.1% of rose essence into the mixed solution of the bacillus subtilis and the streptomyces rochei, and continuously stirring and mixing to obtain a mixed solution.

Dissolving 2% polyvinyl alcohol in a proper amount of hot water at 60-70 ℃, cooling to room temperature after dissolving, adding into the mixed solution, adding distilled water to supplement to 100%, and continuously stirring and mixing to obtain the composite biological seed coating agent.

Comparative example 2

This comparative example differs from example 3 in that no Bacillus subtilis powder was included.

Comparative example 3

This comparative example differs from example 3 in that no paenibacillus polymyxa powder was contained.

Comparative example 4

This comparative example differs from example 3 in that the purple lilac spore powder was not contained.

Comparative example 5

This comparative example is different from example 3 in that metarhizium anisopliae spore powder is not contained.

Comparative example 6

This comparative example differs from example 3 in that the group a fungus powder was not included.

Comparative example 7

This comparative example differs from example 3 in that it does not contain group B fungal powder.

Performance test

The following performance tests were performed on the seed coating samples provided in examples 1-7 and comparative examples 1-7, with the test data shown in table two.

Coating the seed coating agent sample and cucumber seeds at a ratio of 1: 20, and sowing in different pots which are sterilized at high temperature and inoculated with cucumber rhizoctonia solani, damping-off and root-knot nematode respectively. And (3) placing the seeding pot into a 25 ℃ illumination incubator for culture, observing the growth and disease incidence conditions of the cucumber seedlings, and investigating the control effect after culturing for 20 days so as to explore the control effect of different seed coating samples on the pests and diseases of the cucumber in the seedling stage.

The seed coating agent sample is used for treating tomatoes in a mode of coating, seedling raising, field planting and transplanting in a seed ratio of 1: 20, and is used for conventional cultivation and management in a vegetable greenhouse. The average plant height, fruit weight and individual plant yield of the tomatoes treated differently were determined to explore the effect of different seed coatings on the growth and yield of tomatoes.

Table for testing two kinds of seed coating sample

By combining the examples 1-3 and the comparative example 1 and combining the table II, it can be seen that compared with the comparative example 1, the plant rates of the rhizoctonia solani kuhn, the damping-off and the root-knot nematode of the cucumbers in the examples 1-3 are lower, and the flowering phase plant height, the single fruit weight, the single plant yield and the yield increase rate of the examples 1-3 are higher, which indicates that the seed coating sample prepared by the preparation process has better effect of resisting insect diseases and is beneficial to improving the yield of crops.

In combination with examples 3-7 and table two, it can be seen that the plant disease rates of rhizoctonia solani, damping off and root-knot nematode of cucumber in example 4 are slightly reduced, and the flowering plant height, single fruit weight, single plant yield and yield increase rate of example 4 are significantly increased, compared with example 3, which indicates that the addition of amino acid powder, active enzyme and small molecular nutrient is helpful for improving seed growth; compared with example 3, the plant rates of the rhizoctonia solani kuhn, the damping-off and the root-knot nematode of the cucumbers in examples 5-6 are not changed greatly, and the yield is also not changed greatly, which shows that the seed coating samples prepared by the methods have good insect disease resistance under the water content of the examples 3, 5 and 6; compared with example 3, the plant rates of rhizoctonia solani, damping-off and root-knot nematode of cucumber in example 7 are all obviously reduced, and the flowering phase plant height, single fruit weight, single plant yield and yield increase of example 7 are all increased, which shows that the addition of the antifoaming agent before the end of fermentation is helpful for improving the sterilization effect of the A-type fungus powder and the B-type fungus powder.

Combining example 3 and comparative examples 2-7 and combining table two, it can be seen that compared with example 3, the plant diseases of rhizoctonia solani kuhn, damping-off and root-knot nematode of comparative examples 2-7 are all higher, the flowering phase plant height, single fruit weight, single plant yield and yield increase of comparative examples 2-7 are obviously reduced, and compared with comparative examples 1-5, the plant diseases of comparative examples 6 and 7 are higher and the yield increase is smaller, which shows that the combination use of bacillus subtilis powder, paenibacillus polymyxa powder, purple violet spore powder and metarhizium anisopliae spore powder is beneficial to improving the disease resistance effect of seed coating samples.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims

1. A preparation process of a multifunctional microbial organic seed coating agent is characterized by comprising the following preparation steps: uniformly mixing 8-15 parts of A-type bacterium powder, 10-20 parts of B-type bacterium powder, 5-18 parts of yeast powder, 3-5 parts of protein powder, 5-10 parts of corn steep liquor dry powder and 5-10 parts of starch in parts by weight to obtain a multifunctional microbial organic seed coating agent; the A-type bacterium powder is prepared from bacillus strains, and the B-type bacterium powder is prepared from bacteriocidal strains.

2. The preparation process of the multifunctional microbial organic seed coating agent according to claim 1, wherein the preparation process comprises the following steps: the A-type bacterium powder is prepared by the following steps:

3. The preparation process of the multifunctional microbial organic seed coating agent according to claim 2, wherein the preparation process comprises the following steps: the bacillus strain comprises bacillus subtilis and paenibacillus polymyxa, wherein the paenibacillus A powder comprises 3-5 parts by weight of bacillus subtilis powder and 5-10 parts by weight of paenibacillus polymyxa powder.

4. The preparation process of the multifunctional microbial organic seed coating agent according to claim 2, wherein the preparation process comprises the following steps: the separation and spray drying of the bacillus strain fermentation liquor are carried out in a centrifugal drying all-in-one machine, the centrifugal drying all-in-one machine comprises a centrifugal machine body (1), a drying machine body (2) and a rotating component (3), the drying machine body (2) comprises a drying cylinder (21), a centrifugal atomizer (22), a spray head (23) and an air injection component (24), the centrifugal machine body (1) is communicated with the centrifugal atomizer (22), a sealing cover (19) is installed at the lower end of the centrifugal machine body (1), the centrifugal atomizer (22) is installed on the sealing cover (19), the spray head (23) is arranged in the sealing cover (19), the spray head (23) is communicated with the centrifugal atomizer (22), at least two drying cylinders (21) are arranged, the drying cylinders (21) are connected with the rotating component (3), one of the drying cylinders (21) is a working cylinder, the rest drying cylinders (21) are prepared cylinders, the top ends of the working cylinders are abutted to the bottom ends of the sealing covers (19), the spray heads (23) face the inside of the drying cylinders (21), and the air spraying pieces (24) are installed on the drying cylinders (21);

rotating assembly (3) include base (31), rotating tube (32), center pin (33), connecting rod (34) and rotate motor (35), center pin (33) fixed connection is on base (31), rotating tube (32) cover is established on center pin (33), connecting rod (34) fixed connection is on rotating tube (32) periphery wall, the one end and the drying cylinder (21) fixed connection of rotating tube (32) are kept away from in connecting rod (34), fixedly connected with swing pinion (321) on rotating tube (32) periphery wall, it installs on base (31) to rotate motor (35), install driving gear (351) on rotating motor (35), driving gear (351) and swing pinion (321) meshing.

5. The preparation process of the multifunctional microbial organic seed coating agent according to claim 4, wherein the preparation process comprises the following steps: fixedly connected with thrust cylinder (191) on sealed lid (19), the flexible end fixedly connected with sealing ring (192) of thrust cylinder (191), sealing ring (192) cover is established on sealed lid (19), fixedly connected with seal ring (193) on the internal perisporium of sealing ring (192), the periphery wall of sealed lid (19) and drying cylinder (21) all with the internal perisporium butt of seal ring (193).

6. The preparation process of the multifunctional microbial organic seed coating agent according to claim 4, wherein the preparation process comprises the following steps: the centrifuge body (1) comprises a base (11), a rotary drum (12), a telescopic piston (13), a liquid discharge pipe (14) and a slag discharge pipe (15), wherein the rotary drum (12) is rotatably installed on the base (11), a centrifugal motor (16) for driving the rotary drum (12) is arranged on the base (11), the slag discharge pipe (15) comprises a first slag discharge pipe (151), a second slag discharge pipe (152) and a threaded ring (153), the first slag discharge pipe (151) is communicated with the bottom end of the rotary drum (12), the threaded ring (153) is in threaded connection with the bottom end of the first slag discharge pipe (151), the top end of the second slag discharge pipe (152) is in threaded connection with one end, far away from the first slag discharge pipe (151), of the threaded ring (153), the other end of the second slag discharge pipe (152) is fixedly connected with a sealing cover (19), and both the spray head (23) and the centrifugal atomizer (22) are communicated with the second slag discharge pipe (152), the liquid discharge pipe (14) and the slag discharge pipe (151) are both provided with control valves (4); the telescopic piston (13) comprises a power motor (131), a piston head (132), a rack bar (133), a piston rod (134) and a power gear (135), the power motor (131) is fixedly arranged on the base (11), the power gear (135) is arranged at the output end of the power motor (131), the rack bar (133) is connected on the machine base (11) in a sliding way along the vertical direction, the power gear (135) is meshed with the rack bar (133), the top end of the piston rod (134) is rotationally connected with the toothed bar (133), the bottom end of the piston rod (134) is fixedly connected with the piston head (132), the piston head (132) is in sliding contact with the inner peripheral wall of the rotary drum (12), the lower surface of the piston head (132) is rotatably connected with a scraping blade (17), the piston head (132) is provided with a driving motor (18) for driving the scraping blade (17) to rotate.

7. The preparation process of the multifunctional microbial organic seed coating agent according to claim 2, wherein the preparation process comprises the following steps: the B-type bacterium powder is prepared by the following steps:

culturing the bactericidal strains until spore is produced, and inoculating the bactericidal strains after spore production into a B-type fermentation tank after sterilization;

fermenting the B-type fermentation tank for 3-5 days under the conditions of 120-;

8. The preparation process of the multifunctional microbial organic seed coating agent of claim 6, wherein the preparation process comprises the following steps: the sterilization bacterial strain comprises lilium purple spore powder and metarhizium anisopliae, and the B type bacterial powder comprises 5-10 parts by weight of lilium purple spore powder and 5-10 parts by weight of metarhizium anisopliae spore powder.

9. The preparation process of the multifunctional microbial organic seed coating agent according to claim 7, wherein the preparation process comprises the following steps: adding 1-2 parts by weight of defoaming agent into the A-type fermentation tank or the B-type fermentation tank 0-12h before the fermentation process in the A-type fermentation tank or the B-type fermentation tank is finished.

10. The preparation process of the multifunctional microbial organic seed coating agent according to claim 1, wherein the preparation process comprises the following steps: also comprises 5-10 parts of amino acid powder, 10-15 parts of active enzyme and 5-10 parts of nutrient containing trace elements.