CN113008785A - Optical detection device and protein detection device using same - Google Patents
Optical detection device and protein detection device using same Download PDFInfo
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- CN113008785A CN113008785A CN201911329585.0A CN201911329585A CN113008785A CN 113008785 A CN113008785 A CN 113008785A CN 201911329585 A CN201911329585 A CN 201911329585A CN 113008785 A CN113008785 A CN 113008785A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
- G01N2021/513—Cuvettes for scattering measurements
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Abstract
The invention discloses an optical detection device and a protein detection device using the same. By the mode, the light source can reduce the phenomenon that the light beam is reflected back to the light source again, reduce the loss of the light source and prolong the service life of the light source.
Description
Technical Field
The invention relates to the field of biological detection, in particular to an optical detection device and a protein detection device using the same.
Background
The detection of specific proteins is a very hot clinical test item in recent years, including CRP, SAA and the like, and the detection principle is an optical colorimetric method.
The existing detection device generally comprises a light source and a colorimetric pool, wherein the light source is used for emitting parallel light beams to vertically enter the colorimetric pool, so that part of the parallel light beams are reflected by an original path on the pool wall of the colorimetric pool and enter the light source again, the light source is damaged, the output power of the light source is influenced, the detection stability is further influenced, and the service life of the light source can be shortened.
Disclosure of Invention
The invention mainly provides an optical detection device and a protein detection device using the same. The problem of light source damage caused by parallel light beam reflection in the prior art is solved.
In order to solve the technical problems, the invention adopts a technical scheme that: the utility model provides an optical detection device, detection device includes light source and colorimetric pool, the light source is used for the transmission to assemble the light beam, the colorimetric pool set up in assemble the light path of light beam and with the optical axis that assembles the light beam is the slope setting.
According to an embodiment provided by the present invention, the detection device further includes a transmission light collector and/or a scattering light collector, the transmission light collector is disposed on the optical axis of the converged light beam and is configured to collect the light beam transmitted through the cuvette, and an optical collection channel of the scattering light collector forms a predetermined included angle with the optical axis of the converged light beam and is configured to collect the light beam scattered through the cuvette.
According to an embodiment of the present invention, the detection device further includes a first diaphragm, and the first diaphragm includes a transmission aperture, and the transmission aperture is disposed between the cuvette and the transmission light collector and located at a beam waist position of the converged light beam.
According to an embodiment of the present invention, the transmission aperture is a circular aperture, and a diameter of the transmission aperture is equal to a beam waist diameter of the converged light beam.
According to an embodiment of the present invention, the first diaphragm further includes a scattered light hole, the scattered light hole is disposed between the cuvette and the scattered light collector, and the scattered light hole is an elliptical light hole.
According to an embodiment of the present invention, the optical detection device further includes a first extinction groove and a first lens sequentially disposed between the transmission aperture and the transmission light collector, and a second extinction groove and a second lens sequentially disposed between the scattering aperture and the scattering light collector.
According to an embodiment of the present invention, the optical detection device further includes a second diaphragm and a third diaphragm sequentially disposed between the light source and the cuvette.
According to an embodiment of the disclosure, the aperture of the second diaphragm is larger than the aperture of the third diaphragm.
According to an embodiment provided by the invention, the optical detection device further comprises a substrate, and the light source, the colorimetric pool, the transmitted light collector and the scattered light collector are all arranged on the substrate.
In order to solve the technical problem, the invention adopts another technical scheme that: there is provided a protein detection device comprising the optical detection device of any one of the above.
The invention has the beneficial effects that: be different from prior art's condition, assemble the light beam through the light source transmission for the most light beam of incident colorimetric pool is not vertical incidence, thereby can reduce the colorimetric pool and reflect the original way of assembling the light beam, and further through with the colorimetric pool with assemble the optical axis slope setting of light beam, reduction colorimetric pool that can be further is to assembling the original way reflection of light beam, and then the reduction is because the light beam of colorimetric pool reflection enters into the light source, thereby reduce the loss to the light source, and then improve the life-span of light source.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a schematic structural diagram of a first embodiment of an optical inspection apparatus provided in the present invention;
FIG. 2 is a schematic structural diagram of a second embodiment of the optical inspection apparatus provided in the present invention;
FIG. 3 is a schematic structural diagram of a third embodiment of an optical inspection apparatus according to the present invention;
FIG. 4 is a schematic structural diagram of a fourth embodiment of an optical inspection apparatus according to the present invention;
FIG. 5 is a schematic structural diagram of a fifth embodiment of an optical inspection device according to the present invention;
FIG. 6 is a schematic structural diagram of a sixth embodiment of an optical inspection device according to the present invention;
fig. 7 is a schematic structural diagram of a seventh embodiment of the optical detection apparatus according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides an optical inspection apparatus 10, the optical inspection apparatus 10 includes a light source 100 and a cuvette 200, the light source 100 is used for emitting a converged light beam, and the cuvette 200 is disposed on a light path of the converged light beam and inclined from an optical axis of the converged light beam. Specifically, the cuvette 200 is disposed at a side wall 210 of the light incident surface facing the light source 100, and forms an oblique angle with the optical axis of the converged light beam.
It should be noted that the converged light beam shown in fig. 1 is schematic in cross section, and in a perspective view, a plurality of light beams of the converged light beam converge around an optical axis. And specifically, the light source 10 may be an independent light source capable of emitting a convergent light beam, or a combined light source of a light emitter and a lens capable of emitting a parallel light beam.
In particular, a converging light beam refers to a point or a small area where a plurality of light rays converge in the propagation direction, and the point or the area can be referred to as the beam waist position of the converging light beam.
As shown in fig. 1, in one embodiment, the entire cuvette 200 may be a regular cylinder, and the entire cuvette 200 is disposed at an oblique angle with respect to the optical axis.
In another embodiment, as shown in fig. 2, the light-incident side wall 210 of the cuvette 200 is inclined relative to the bottom wall 220 of the cuvette 200 such that the light-incident side wall 210 is inclined relative to the optical axis when the cuvette 200 is positioned generally horizontally relative to the optical axis.
As shown in fig. 1 and 2, the inclination angle α of the inclination is 1 ° or more and 5 ° or less.
In the above embodiment, the light source 100 emits the converging light beam, so that most of the light beam entering the colorimetric pool 200 is not vertically incident, thereby reducing the original reflection of the colorimetric pool 200 on the converging light beam, and further setting the colorimetric pool 200 and the optical axis of the converging light beam in an inclined manner, further reducing the original reflection of the colorimetric pool 200 on the converging light beam, and further reducing the loss of the light source 100 due to the fact that the light beam reflected by the colorimetric pool 200 enters the light source 100, thereby prolonging the service life of the light source 100. Furthermore, if the light beam is reflected into the light source 100, the stability of the output power of the light source 100 is directly affected, and the stability of the whole detection result is further affected, the invention reduces the influence of the light beam on the light source 100 by reducing the light beam reflected into the light source 100, thereby ensuring that the light source 100 continuously outputs the light beam with stable power, and further improving the stability of the detection result.
Referring to fig. 3, the optical detection apparatus 10 further includes a transmitted light collector 300 and/or a scattered light collector 400, and specifically, only the transmitted light collector 300 may be provided, or only the scattered light collector 400 may be provided, or both the transmitted light collector 300 and the scattered light collector 400 are provided, which is not limited in detail herein. The transmitted light collector 300 is disposed on an optical axis of the converged light beams and is configured to collect light beams transmitted through the cuvette 200, and may generate transmitted electrical signals according to the collected light beams. The light collection channel of the scattered light collector 400 and the optical axis of the converged light beam form a preset included angle and are used for collecting the light beam scattered by the cuvette 200, and a scattered electric signal can be generated according to the collected light beam.
In a specific scenario, the detection of the solution in the cuvette 200 generally includes a scattering method and a transmission method, wherein the scattering method is suitable for a scenario in which the concentration of the solution is low, and the transmission method is suitable for a scenario in which the concentration of the solution is high.
In a specific embodiment, a transmission concentration value and a scattering concentration value of the solution in the cuvette 200 are respectively obtained through the transmission light signal generated by the transmission light collector 300 and the scattering light signal generated by the scattering light collector 400, and the transmission concentration value and the scattering concentration value are compared with a preset concentration value, if the transmission concentration value and the scattering concentration value are higher than the preset concentration value, that is, the solution can be considered as a high value, the transmission concentration value is adopted as a final detection result. And if the concentration value is lower than the preset concentration value, namely the solution can be considered as a low value, adopting the scattering concentration value as a final detection result.
In the above embodiment, the transmitted light collector 300 is arranged to collect the light beams transmitted through the cuvette 200 and the scattered light collector 400 is arranged to collect the light beams scattered through the cuvette 200, so that the transmission concentration value and the transmission concentration value of the solution in the cuvette 200 can be detected, and further the transmission concentration value or the scattering concentration value can be selected as the final detection result according to the detection result, thereby ensuring that the optimal detection result can be ensured regardless of the high concentration solution or the low concentration solution.
Referring to fig. 4, the optical detection device 10 further includes a first diaphragm 500, the first diaphragm 500 includes a transmission aperture 510, and the transmission aperture 510 is disposed between the cuvette 200 and the transmission light collector 300 and located at a beam waist position of the converged light beam.
As shown in fig. 4, after the converged light beam enters the cuvette 200, a part of the converged light beam directly transmits through the cuvette 200 and is transmitted along the original optical path, a part of the converged light beam is scattered by the solution of the cuvette 200, the light beam to be collected by the transmitted light collector 300 is the part directly transmitting through the cuvette 200, and a transmitted electrical signal is generated according to the light beam, and if the stray light scattered due to the inner wall of the cuvette 200 is collected by the transmitted light collector 300, since the stray light does not carry any signal of the solution of the cuvette 200, the accuracy of the transmitted electrical signal is affected, and the detection result is ultimately affected. According to the invention, by adopting the converged light beam and arranging the transmission light hole 510 at the beam waist position of the converged light beam, as the part from the light path of the converged light beam to the beam waist position is gradually narrowed, and compared with a parallel light path, the light path is narrowed, which means less stray light can be parallel to the light path, and further by arranging the transmission light hole 510 at the beam waist position, only the light along the light path of the original converged light beam can pass through the transmission light hole 510 and be collected by the transmission light collector 300, and the most stray light can be blocked, so that the stray light collected by the transmission light collector 300 can be reduced, and the accuracy of the detection result can be greatly improved.
In a specific embodiment, the transmission light hole 510 may be a circular light hole, and the diameter of the transmission light hole 510 is equal to the diameter of the beam waist of the converged light beam.
In other embodiments, the diameter of the light transmitting hole 510 may be reduced according to the light intensity of the light to be collected by the light transmitting collector 300.
In other embodiments, the diameter of the light transmitting hole 510 may be further increased according to assembly tolerance, and the like, which are not limited herein.
As shown in fig. 4, the first diaphragm 500 further includes a scattered light hole 520, the scattered light hole 520 is disposed between the cuvette 200 and the scattered light collector 400, and the scattered light hole is an elliptical light hole. Wherein the major axis of the scattered light hole is determined by the upper and lower limits of the scattered light to be collected by the scattered light collector 400, and the minor axis of the scattered light hole is equal to the diameter of the converged light beam when entering the cuvette 200.
The scattered light entering the scattered light collector 400 through the scattered light hole 520 can satisfy a certain angle consistency by arranging the scattered light hole 520, so that the accuracy of the detection result is improved.
As shown in fig. 5, the optical detection device 10 further includes a first extinction groove (not shown), a first lens 610, a second extinction groove (not shown), and a second lens 620.
Wherein the first extinction groove and the first lens 610 are sequentially arranged between the transmission aperture 510 and the transmission light collector 300, the first extinction groove and the first lens 610 are matched to further eliminate stray light entering the transmission light collector 300, and further, the light spot entering the transmission light collector 300 can be controlled by arranging the first lens 610, i.e. the size of the light spot can be restricted, so that the light spot has better shape and size when being collected by the transmission light collector 300.
The second extinction groove and the second lens 620 are sequentially disposed between the scattered light aperture 520 and the scattered light collector 400, and the second extinction groove and the second lens 620 cooperate to eliminate stray light entering the transmitted light collector 300, and can restrict the size of a light spot, so as to improve the accuracy of a detection result.
As shown in fig. 6, the optical detection device 10 further includes a second diaphragm 710 and a third diaphragm 720 sequentially disposed between the light source 100 and the cuvette 200. And the aperture diameter of the second diaphragm 710 is larger than that of the third diaphragm 720.
In a specific embodiment, the distance between the aperture of the second diaphragm 710 and the light source 100 is greater than or equal to 5mm, and less than or equal to 10 mm. Specifically, the distance from the light exit hole of the light source 100 is greater than or equal to 5mm, and less than or equal to 10 mm. Specifically, it may be 5mm, 7mm or 10mm, and is not particularly limited herein.
The distance between the light hole of the third diaphragm 720 and the light hole of the second diaphragm 710 is greater than or equal to 30mm and less than or equal to 40 mm; specifically, it may be 30mm, 35mm or 40mm, and is not particularly limited herein. The distance between the cuvette 200 and the aperture of the third diaphragm 720 is greater than or equal to 2mm and less than or equal to 5 mm. Specifically, it may be 2mm, 4mm or 5mm, and is not particularly limited herein.
Specifically, the converged light beam can be constrained by keeping the aperture of the second diaphragm 710 and the aperture of the third diaphragm 720 consistent with the optical path change of the converged light beam. On the other hand, stray light generated by the light source 100 itself or the optical cavity between the light source 100 and the third diaphragm 720 can be prevented from entering the cuvette 200, and further, because the aperture of the third diaphragm 720 is small, the influence of the stray light reflected by the cuvette 200 and the transmitted light collector 300 on the light source 100 can be further reduced, so that the light source 100 is protected.
As shown in fig. 7, the optical detection device 10 further includes a base 800, wherein the light source 100, the cuvette 200, the transmitted light collector 300, and the scattered light collector 400 are disposed on the base 800. In other embodiments, other optical components of the optical detection apparatus 10, such as the first stop 500, the second stop 710, the third stop 720, the first extinction groove, the first lens 610, the second extinction groove, and the second lens 620, may also be disposed on the substrate 800, which is not limited herein.
A description of a specific scenario is made with respect to the overall structure of the optical detection apparatus 10:
the light source 100 emits a converged light beam, the converged light beam sequentially passes through the second diaphragm 710 and the third diaphragm 720, the converged light beam is constrained by the second diaphragm 710 and the third diaphragm 720 through respective light holes, so that the converged light beam is incident into the cuvette 200 according to a required cross section size, because the cuvette 200 and the optical axis of the converged light beam have a certain inclination, the converged light beam is not easy to reflect back to the light source 100 along the original path, and part of the light beam reflected towards the light source 100 is blocked by the second diaphragm 710 and the third diaphragm 720, so as to protect the light source 100, the part of the converged light beam incident into the cuvette 100 is scattered due to the inner wall of the cuvette 100 to form stray light, part of the light beam directly transmits through the cuvette 100 to form transmitted light, and part of the light beam forms scattered light due to the influence of a solution in the cuvette.
The transmitted light sequentially passes through the transmission aperture 510, the first extinction groove and the first lens 610 of the first diaphragm 500 and is collected by the transmitted light collector 300, specifically, on one hand, since the light path of the converged light beam in the propagation process is gradually narrowed, the stray light and the scattered light parallel to the converged light beam can be reduced, and further the transmission aperture 510 is arranged at the beam waist position of the converged light beam, so that only the light beam coincident with the light path of the converged light beam can penetrate through the transmission aperture 510, then the stray light entering the transmitted light collector 300 is further eliminated through the cooperation of the first extinction groove and the first lens 610, and the light beam is constrained by the first lens 610, so that the transmitted light collector 300 has a better shape and size when being collected. In the above-mentioned structure, on the one hand can reduce the light beam and reflect back to light source 100 to protect light source 100, improve the stability of the light beam that assembles that light source 100 launches, and then improve detection effect, on the other hand is through handling stray light etc. to reduce stray light etc. and enter into transmitted light collector 300, thereby reduce the influence of stray light to the detection, thereby improve detection effect.
The scattered light sequentially passes through the scattered light aperture 520, the second extinction groove and the second lens 620 of the first diaphragm 500 and is collected by the scattered light collector 400, the angle consistency of the scattered light entering the scattered light collector 400 can be ensured through the scattered light aperture 520, the stray light is reduced through the second extinction groove and the second lens 620, and therefore the whole detection effect can be improved.
In particular embodiments, the present invention also provides a method of detecting contamination of the cuvette 200.
S11, the transmitted light collector 300 collects the transmitted light of the light beam with the preset power emitted by the light source 100 via the cuvette 200, and obtains the signal intensity according to the transmitted light.
In a specific scenario, after the dilution liquid is added to the cuvette 200, the light source 100 emits a light beam to the cuvette 200 and passes through the dilution liquid, and the light beam is collected by the transmitted light collector 300, and the signal intensity is determined according to the collected transmitted light, and if the signal intensity is within a first preset range, the power emitted by the light source 100 at that time is used as the preset power.
After the cuvette 200 is put into use, a diluent can be added into the cuvette 200, a light beam with a preset power is emitted by the light source 100, the transmitted light collector 300 collects the transmitted light of the light beam with the preset power passing through the cuvette 200, and the signal intensity is determined according to the transmitted light.
And S12, judging the threshold range of the signal intensity, and determining the detection result according to the threshold range.
After the signal strength is obtained, the threshold range of the signal strength may be determined, and specifically, the threshold range includes a first preset range, a second preset range, and a third preset range. The third preset range is a total range of signal intensities that can be acquired by the transmitted light acquirer 300. The second preset range is within a third preset range and is not superposed with the upper limit and the lower limit of the third preset range, and the first preset range is within the second preset range.
In a particular embodiment, the first set of predetermined ranges may be denoted as [ C, D ], the second set of predetermined ranges may be denoted as [ E, F ], and the third set of predetermined ranges may be denoted as [ a, B ]. Then A is more than E and less than C and less than D and less than F and less than B.
If the signal intensity is within a first predetermined range, it may indicate that the cuvette 200 is of a higher cleanliness. If the signal intensity is outside of the first preset range and within a second preset range, indicating that the cuvette 200 has been lightly contaminated, automated cleaning maintenance may be performed on the cuvette 200.
Further, after the color cell 200 is subjected to the automatic cleaning maintenance and the detection process of S11 is repeated, if the signal intensity is still outside the first predetermined range and within the second predetermined range, it indicates that the color cell 200 may be seriously contaminated.
If the signal intensity is outside the second predetermined range, it is indicative that the cuvette 200 may be heavily contaminated.
Through the embodiment, the invention can carry out pollution detection on the colorimetric pool 200 so as to determine whether the colorimetric pool 200 can be continuously used, so that the subsequent detection result is not influenced.
In particular embodiments, the present disclosure also provides a method of using light source 100.
In a specific embodiment, the transmitted light of the light beam with the preset power emitted by the light source 100 through the cuvette 200 may be collected by the transmitted light collector 300, and the signal intensity may be obtained according to the transmitted light. Wherein the cuvette 200 contains no solution or only a diluent. Subsequently, by determining whether the signal intensity is smaller than the preset threshold, if the signal intensity is smaller than the preset threshold, it represents that the light source 100 may be damaged, and the maintenance is required.
The invention also provides a protein detection device, which comprises the optical detection device in any embodiment.
Specifically, the protein detection device may be configured to obtain a transmission concentration value and a scattering concentration value of the solution in the cuvette 200 according to the transmission light signal generated by the transmission light collector 300 and the scattering light signal generated by the scattering light collector 400, respectively, and compare the transmission concentration value and the scattering concentration value with a preset concentration value, if the transmission concentration value and the scattering concentration value are higher than the preset concentration value, that is, the solution may be considered as a high value, and then adopt the transmission concentration value as a final detection result. And if the concentration value is lower than the preset concentration value, namely the solution can be considered as a low value, adopting the scattering concentration value as a final detection result.
In summary, the present invention provides an optical detection device and a protein detection device using the same. The light beams are emitted and converged through the light source 100, most light beams incident into the colorimetric pool 200 are not vertical incidence, the original path reflection of the colorimetric pool 200 to the converged light beams can be reduced, the colorimetric pool 200 and the optical axis of the converged light beams are further obliquely arranged, the original path reflection of the colorimetric pool 200 to the converged light beams can be further reduced, the light beams reflected by the colorimetric pool 200 are further reduced to enter the light source 100, the loss of the light source 100 is reduced, and the service life of the light source 100 is prolonged. Furthermore, by arranging the transmission light collector 300 for collecting the converged light beams transmitted through the cuvette 200 and the scattered light collector 400 for collecting the converged light beams scattered through the cuvette 200, the detection of the transmission concentration value and the transmission concentration value of the solution in the cuvette 200 can be performed, and further the transmission concentration value or the scattering concentration value can be selected as the final detection result according to the detection result, thereby ensuring that the optimal detection result can be ensured regardless of the high concentration solution or the low concentration solution. Furthermore, by adopting the converged light beam and arranging the transmission light aperture 510 at the beam waist position of the converged light beam, since the part from the light path of the converged light beam to the beam waist position is gradually narrowed, the light path is narrowed, which means less stray light can be parallel to the light path, and further by arranging the transmission light aperture 510 at the beam waist position, only the light along the original light path of the converged light beam can pass through the transmission light aperture 510 and be collected by the transmission light collector 300, and is enough to block most of the stray light, so that the stray light collected by the transmission light collector 300 can be reduced, and the accuracy of the detection result can be greatly improved.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. The utility model provides an optical detection device, its characterized in that, detection device includes light source and colorimetric pool, the light source is used for the transmission to assemble the light beam, the colorimetric pool set up in assemble the light path of light beam and with the optical axis that assembles the light beam is the slope setting.
2. The optical detection device according to claim 1, wherein the detection device further comprises a transmission light collector and/or a scattering light collector, the transmission light collector is disposed on the optical axis of the converged light beam and is used for collecting the light beam transmitted through the cuvette, and an optical collection channel of the scattering light collector and the optical axis of the converged light beam form a preset included angle and is used for collecting the light beam scattered through the cuvette.
3. The optical detection device according to claim 2, further comprising a first diaphragm, wherein the first diaphragm comprises a transmission aperture, and the transmission aperture is disposed between the cuvette and the transmission light collector and located at a beam waist position of the converged light beam.
4. The optical inspection device of claim 3, wherein the transmissive optical aperture is a circular aperture and has a diameter equal to a beam waist diameter of the converging light beam.
5. The optical detection device according to claim 3, wherein the first diaphragm further comprises a scattered light hole, the scattered light hole is disposed between the cuvette and the scattered light collector, and the scattered light hole is an elliptical light hole.
6. The optical detection device according to claim 5, further comprising a first extinction groove and a first lens sequentially disposed between the transmission aperture and the transmission light collector, and a second extinction groove and a second lens sequentially disposed between the scattering aperture and the scattering light collector.
7. The optical detection device of claim 1, further comprising a second diaphragm and a third diaphragm sequentially disposed between the light source and the cuvette.
8. The optical inspection device of claim 7, wherein the aperture diameter of the second aperture is larger than the aperture diameter of the third aperture.
9. The optical detection device according to claim 2, further comprising a substrate, wherein the light source, the cuvette, the transmitted light collector, and the scattered light collector are disposed on the substrate.
10. A protein detection device, characterized in that the detection device comprises the optical detection device according to any one of claims 1 to 9.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911329585.0A CN113008785A (en) | 2019-12-20 | 2019-12-20 | Optical detection device and protein detection device using same |
| PCT/CN2020/136599 WO2021121231A1 (en) | 2019-12-20 | 2020-12-15 | Optical testing device and protein testing device using same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911329585.0A CN113008785A (en) | 2019-12-20 | 2019-12-20 | Optical detection device and protein detection device using same |
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| CN113008785A true CN113008785A (en) | 2021-06-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE60200139T2 (en) * | 2002-06-11 | 2004-09-30 | Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto | Device with reduced loss due to back reflection |
| CN203758905U (en) * | 2013-11-04 | 2014-08-06 | 叶金友 | Multi-light source light path system of channel of colorimetric pool and detection device |
| WO2016002570A1 (en) * | 2014-07-01 | 2016-01-07 | 株式会社村田製作所 | Laser processing mask |
| CN105784642A (en) * | 2016-05-23 | 2016-07-20 | 深圳市国赛生物技术有限公司 | Detection device and optical system thereof |
| CN211927663U (en) * | 2019-12-20 | 2020-11-13 | 深圳市帝迈生物技术有限公司 | Optical detection device and protein detection device using same |
-
2019
- 2019-12-20 CN CN201911329585.0A patent/CN113008785A/en active Pending
-
2020
- 2020-12-15 WO PCT/CN2020/136599 patent/WO2021121231A1/en active Application Filing
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| WO2021121231A1 (en) | 2021-06-24 |
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