CN113005178B - 一种rna文库的构建方法及试剂盒 - Google Patents
一种rna文库的构建方法及试剂盒 Download PDFInfo
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- CN113005178B CN113005178B CN202110493902.3A CN202110493902A CN113005178B CN 113005178 B CN113005178 B CN 113005178B CN 202110493902 A CN202110493902 A CN 202110493902A CN 113005178 B CN113005178 B CN 113005178B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
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- General Chemical & Material Sciences (AREA)
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Abstract
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CN202110493902.3A CN113005178B (zh) | 2021-05-07 | 2021-05-07 | 一种rna文库的构建方法及试剂盒 |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103667481A (zh) * | 2013-12-06 | 2014-03-26 | 上海美吉生物医药科技有限公司 | 用于对已知序列两侧未知侧翼序列测序的方法 |
CN108350492A (zh) * | 2015-11-05 | 2018-07-31 | 通用电气公司 | 连接酶辅助的核酸环化和扩增 |
CN109563541A (zh) * | 2016-06-30 | 2019-04-02 | 格里尔公司 | 用于制备无细胞dna/rna定序库的rna差异性标记方法 |
CN112176422A (zh) * | 2020-10-19 | 2021-01-05 | 天津诺禾致源生物信息科技有限公司 | Rna文库的构建方法 |
CN112626176A (zh) * | 2021-03-10 | 2021-04-09 | 翌圣生物科技(上海)有限公司 | 用于rna建库中快速去除靶rna的逆转录阻碍探针及其应用 |
-
2021
- 2021-05-07 CN CN202110493902.3A patent/CN113005178B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103667481A (zh) * | 2013-12-06 | 2014-03-26 | 上海美吉生物医药科技有限公司 | 用于对已知序列两侧未知侧翼序列测序的方法 |
CN108350492A (zh) * | 2015-11-05 | 2018-07-31 | 通用电气公司 | 连接酶辅助的核酸环化和扩增 |
CN109563541A (zh) * | 2016-06-30 | 2019-04-02 | 格里尔公司 | 用于制备无细胞dna/rna定序库的rna差异性标记方法 |
CN112176422A (zh) * | 2020-10-19 | 2021-01-05 | 天津诺禾致源生物信息科技有限公司 | Rna文库的构建方法 |
CN112626176A (zh) * | 2021-03-10 | 2021-04-09 | 翌圣生物科技(上海)有限公司 | 用于rna建库中快速去除靶rna的逆转录阻碍探针及其应用 |
Non-Patent Citations (1)
Title |
---|
食源性致病菌核糖体RNA高效去除体系的建立;黄佳莹等;《南京农业大学学报》;20191231;第42卷(第2期);358-364 * |
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Effective date of registration: 20230914 Address after: 318000 Baita Industrial Cluster, Baita Town, Xianju County, Taizhou City, Zhejiang Province Patentee after: Tanyin medical technology (Zhejiang) Co.,Ltd. Patentee after: Shanghai Tanyin Medical Laboratory Co.,Ltd. Address before: 317300 Baita Industrial Cluster, Baita Town, Xianju County, Taizhou City, Zhejiang Province Patentee before: Tanyin medical technology (Zhejiang) Co.,Ltd. |
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Denomination of invention: A Method and Kit for Constructing RNA Library Granted publication date: 20230811 Pledgee: Agricultural Bank of China Limited Shanghai Yangtze River Delta Integrated Demonstration Zone Sub branch Pledgor: Shanghai Tanyin Medical Laboratory Co.,Ltd. Registration number: Y2024310000181 |
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Granted publication date: 20230811 Pledgee: Agricultural Bank of China Limited Shanghai Yangtze River Delta Integrated Demonstration Zone Sub branch Pledgor: Shanghai Tanyin Medical Laboratory Co.,Ltd. Registration number: Y2024310000181 |
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