CN112998139A - Additive for ensiling peanut seedlings and ensiling method - Google Patents
Additive for ensiling peanut seedlings and ensiling method Download PDFInfo
- Publication number
- CN112998139A CN112998139A CN202110414765.XA CN202110414765A CN112998139A CN 112998139 A CN112998139 A CN 112998139A CN 202110414765 A CN202110414765 A CN 202110414765A CN 112998139 A CN112998139 A CN 112998139A
- Authority
- CN
- China
- Prior art keywords
- peanut
- silage
- ensiling
- peanut vine
- additive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a peanut seedling silage additive and a silage method, and aims to solve the technical problem that the quality and the nutritional value of the current peanut seedling silage are poor. The peanut vine silage additive contains 300-400 g/L of soluble sugar and 4 x 10 lactic acid bacteria9～4×1010cfu/L or/and complex enzyme 2X 104～2.2×104U/L. The silage additive is applied to the silage process of peanut seedlings, prolongs the storage period of fresh peanut seedling coarse fodder through the synergistic effect of soluble sugar and lactic acid bacteria or/and an enzyme preparation, simultaneously generates various organic acids and nutrient substances, improves the palatability and the digestion utilization rate of the peanut seedlings, reduces the content of neutral washing fiber and acidic washing fiber, is favorable for increasing the feed intake of livestock and poultry, and achieves the purpose of fully and reasonably utilizing the peanut seedling resource.
Description
Technical Field
The invention relates to the technical field of silage processing, and particularly relates to a peanut seedling silage additive and a silage method.
Background
Peanut (A)Arachis hypogaea) Is an important oil crop and economic crop in China, the peanut planting area is steadily increased along with the adjustment of the agricultural planting structure, and the peanut planting area in China is predicted to reach 4.80 multiplied by 106 hm by 20202. Peanut vine (1)Peanut vine) Is the plant stem and leaf part left after harvesting the seeds, is a main byproduct in the production process of the peanuts, has considerable yield, and has important economic value and ecological benefit when developing and utilizing the resources. Under the condition that the food conflict between people and livestock is continuously excited, the food conflict between people and livestock in China can be relieved by developing and utilizing peanut seedling feed resources, and the method is an effective way for ensuring the sustainable development of the animal husbandry in China.
Ensiling is a storage method for anaerobically storing green feed, and producing organic acid through lactobacillus fermentation to reduce the pH value of the feed, thereby effectively inhibiting the growth and reproduction of undesirable microorganisms and obtaining the feed which can be stored for a long time and reduce the loss of nutrient substances. The silage technology is widely concerned and applied by people because of the advantages of effectively preserving the taste and the nutrient content of the green feed, ensuring the balanced supply of the green feed for the livestock all year round, wide raw material source and the like. With the continuous improvement and development of the ensiling technology, ensiling fermentation becomes an important production technology for guaranteeing the healthy development of the breeding industry in China.
The peanut vine belongs to leguminous forage, is rich in nutrient components, is rich in crude protein, crude fat, various mineral substances and vitamins, is soft in texture, good in palatability, high in digestibility and low in price, is a high-quality crude feed resource, and has wide development and utilization values. In recent years, peanut seedlings are gradually applied to animal production, and ruminants have better utilization rate of nutrient components of the peanut seedlings due to special physiological structures of the ruminants, so that application research on the ruminant is more intensive. However, the nutritional quality (crude protein content, crude fiber content and the like) of the peanut seedlings is easily affected by various factors and is unstable, the fresh peanut seedling ensiling can better preserve the nutritional ingredients of the raw materials, reduce the loss caused by airing and reduce the risk of mycotoxin generation easily in the hay storage process, but the current single peanut seedling ensiling effect is poor, and effective measures need to be taken to improve the comprehensive quality and the nutritional value of the peanut seedling ensiling feed.
Disclosure of Invention
The invention provides a peanut seedling silage additive and a silage method, and aims to solve the technical problem that the quality and the nutritional value of the current peanut seedling silage are poor.
Based on long-term experimental research and production practice, the invention discovers that the low content of water-soluble saccharides in the peanut seedlings is an important reason causing poor natural silage effect of the peanut seedlings, and the additive with proper components is added in a targeted manner in the silage process, so that the invention is an effective measure for improving the comprehensive quality of silage.
In view of the above, in order to solve the above technical problems, the present invention adopts the following technical solutions:
designing a peanut seedling silage additive containing 300-400 g/L of soluble sugar and 4 x 10 of lactic acid bacteria9～4×1010cfu/L or/and complex enzyme 2X 104～2.2×104 U/L。
The lactobacillus is derived from lactobacillus agent produced by Raman animal nutrition limited company, and the lactobacillus agent contains lactobacillus plantarum more than or equal to 5.0 multiplied by 1010cfu/g, Pediococcus acidilactici ≥ 1.5 × 1010 cfu/g。
The compound enzyme is derived from a compound enzyme preparation, the compound enzyme preparation is composed of neutral cellulase, cellobiase and pectinase according to the volume ratio of 2:1:1, and the enzyme activity is as follows: the neutral cellulase is more than or equal to 2500U/mL, the cellobiase is more than or equal to 1000U/mL, and the pectinase is more than or equal to 15000U/mL.
The soluble sugar is at least one of molasses, glucose, starch and sucrose.
A peanut seedling ensiling method is designed, and comprises the following steps:
(1) cutting the harvested fresh peanut seedlings into grass fragments for later use;
(2) spraying the peanut seedling silage additive to the peanut seedling material obtained in the previous step;
(3) and (3) carrying out vacuum packaging and sealing on the material obtained by the treatment in the step (2), and ensiling for 35-65 days in a room-temperature and dark environment.
In the step (2), the dosage standard of the peanut seedling silage additive is as follows:
so that each kg of peanut vine material correspondingly contains 7.5-10 g of soluble sugar and the viable count of lactobacillus is more than or equal to 1.3 multiplied by 108500-650U of cfu or/and complex enzyme; alternatively, the first and second electrodes may be,
adding 100-400 ml/kg fresh peanut vine of peanut vine silage additive.
In the step (2), the moisture content of the material is adjusted to 60-75% after the peanut vine silage enzyme preparation is sprayed.
In the step (1), the stubble height of the fresh peanut seedlings is 3-5 cm when being harvested.
In the step (1), the harvested fresh peanut seedlings are cut into 3-5 cm grass sections by a hay cutter.
Compared with the prior art, the invention has the main beneficial technical effects that:
1. the silage additive prolongs the storage period of fresh peanut seedling coarse fodder through the synergistic effect of the soluble sugar and the lactic acid bacteria or/and the enzyme preparation, simultaneously generates various organic acids and nutrient substances, improves the palatability and the digestion utilization rate of the peanut seedling, reduces the content of neutral washing fiber and acidic washing fiber, is favorable for increasing the feed intake of livestock and poultry, and achieves the purpose of fully and reasonably utilizing the peanut seedling resource.
2. The peanut vine ensiling method has the advantages of high fermentation efficiency, simple and convenient operation and low cost.
3. The silage method of peanut seedlings can be used for storing and prolonging the silage season for a long time, fully and reasonably utilizes the biological resources of the peanut seedlings, promotes the industrialized development of the peanut seedlings, and can be widely applied to the preparation and production of high-quality peanut seedling silage.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but are not intended to limit the scope of the present invention in any way.
The instruments and devices referred to in the following examples are conventional instruments and devices unless otherwise specified; the raw materials of the related reagents are all conventional raw materials in the market if not specifically indicated; the processing and manufacturing methods are conventional methods unless otherwise specified.
It should be noted that the following examples employ the following sources of raw materials:
the silage lactic acid bacteria agent is produced by Raman animal nutrition limited company, and comprises the following main components: lactobacillus plantarum is more than or equal to 5.0 multiplied by 1010 cfu/g, Pediococcus acidilactici ≥ 1.5 × 1010 cfu/g。
The adopted complex enzyme preparation is produced by Xiasangshi industry Co Ltd, and the enzyme activity is 2500U/mL; cellobiase, the enzyme activity is 1000U/mL; pectinase, enzyme activity 15000U/mL.
In addition, the molasses used in the following examples had a purity of 70% and a sugar content of 41%.
The following examples were conducted in the first laboratory for animal production in the muyu county Zhengzhen test base at the university of agriculture in Henan province.
The first embodiment is as follows: peanut seedling ensiling verification test
Peanut seedling ensiling
1. Cutting: harvesting peanut seedlings with the moisture content of 65-75% (mass percentage content) in a peanut harvesting period, harvesting the peanut seedlings with the stubble height of 3-5 cm, and cutting the peanut seedlings into 3-5 cm grass sections by using a hay cutter; randomly dividing the cut peanut seedlings into five groups, namely a control group, an enzyme and bacterium treatment group, a sugar and enzyme treatment group, a sugar and bacterium treatment group and a sugar and enzyme and bacterium treatment group;
2. and (3) experimental treatment design: adding reagents into the cut peanut seedling grass sections according to the following modes:
control group (CK group): sterile water (25 ml/kg peanut vine material) was added in an equal volume to the following treatment group additives.
Enzyme + bacteria treatment group (EP-L group): adding lactobacillus and complex enzyme preparation into water to obtain mixed solution, and uniformly spraying the mixed solution on peanut seedling material to make every kilogram of peanut seedling material correspond to about 1.0 × 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici, 125U cellulase, 25U cellobiase and 375U pectinase.
Sugar + enzyme treatment group (M-EP group): adding molasses and complex enzyme preparation into water to obtain mixed solution, and uniformly spraying the mixed solution on peanut vine material to make every kilogram of peanut vine material be correspondent to about 10g of molasses and 1.0X 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici.
Sugar + bacteria treatment group (M-L group): adding molasses and lactic acid bacteria agent into water to obtain mixed solution, and uniformly spraying the obtained mixed solution on peanut vine materials to ensure that each kilogram of peanut vine materials corresponds to about 10g of molasses, 125U cellulase, 25U cellobiase and 375U pectinase.
Sugar + enzyme + bacteria treatment group (M-EP-L group): adding molasses, a complex enzyme preparation and a lactic acid bacteria agent into water to obtain a mixed solution, uniformly spraying the mixed solution on peanut vine materials to ensure that each kilogram of peanut vine materials corresponds to about 10g of molasses, 125U cellulase, 25U cellobiase, 375U pectinase and 1.0 multiplied by 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici.
The amount of water added was equal for each group.
3. And (3) storage: and (3) fully and uniformly mixing the peanut seedlings in each group and the additive, filling the mixture into polyethylene bags (each bag is filled with about 800kg of the mixture), repeating the steps for four groups, vacuumizing and sealing the bags by using a vacuum sealing machine, and storing the bags at room temperature (25 +/-2 ℃) for 60 days to obtain the silage peanut seedlings.
(II) fermentation quality analysis of silage peanut seedlings
Randomly taking 20g of silage peanut seedlings from each group as samples to be detected by using sterile tweezers, taking four samples from each group, and detecting related indexes according to the following method:
pH value: about 20g of sample was taken, 180 ml of distilled water was added, and the mixture was stirred manually with a glass rod for 5 min, and its pH value was measured with a pH measuring instrument (Raymond PHS-3C precision pH meter, Shanghai precision scientific Instrument Co., Ltd.).
And (3) organic acid determination: weighing 20g of the extract, pouring the extract into a 200 ml conical flask with a plug, adding 180 ml of distilled water, slightly shaking, leaching in a refrigerated cabinet at 4 ℃ for 24 hours, and shaking uniformly on time; taking out, filtering with filter paper, adding the filtrate into 10 ml test tube, and preserving at-20 deg.C. By GermanySykamAnd (3) analyzing the contents of Lactic Acid (LA), Acetic Acid (AA), Propionic Acid (PA) and Butyric Acid (BA) by ion chromatography, wherein the working temperature is 30 ℃, the flow rate is 1.2 ml/min, and the sample injection amount is 50 mu l.
Determination of ammonia nitrogen content: phenol-hypochlorous acid colorimetric method is adopted.
The results of the measurement of the pH value, the organic acid content and the ammoniacal nitrogen/total nitrogen of each sample to be measured are shown in Table 1.
TABLE 1 comparison of fermentation quality indexes of silage of peanut seedlings obtained from each test group
Note: % DM represents the proportion of organic acids in dry matter; the letters in the same column indicate significant difference (P < 0.05), and the table is as follows; ND means not detected.
The results in table 1 show that, compared with the silage of peanut seedlings alone (a control group), the pH values of the M-EP group, the M-L group and the M-EP-L group are all significantly reduced (P is less than 0.05), the lactic acid contents of the M-L group and the M-EP-L group are significantly higher than those of the control group (P is less than 0.05), and in addition, propionic acid and butyric acid are not detected in each treatment group, so that the silage method improves the fermentation quality of the silage of peanut seedlings and improves the palatability. The content of ammoniacal nitrogen/total nitrogen can reflect the degradation condition of protein and amino acid in the silage, and the lower the content, the better the quality of the silage. The ammonia nitrogen/total nitrogen of the M-EP group, the M-L group and the M-EP-L group are obviously lower than those of other treatment groups (P is less than 0.05), which shows that the combination of molasses and lactic acid bacteria or/and a complex enzyme preparation increases the content of lactic acid, forms an acidic environment, reduces the degradation effect of early plant protease on nitrogen-containing compounds, reduces the content of ammonia nitrogen/total nitrogen, and improves the fermentation quality of silage. Meanwhile, the pH value and the organic acid content of the EP-L group and the ammonia nitrogen/total nitrogen have no obvious difference with the CK group, and further the soluble sugar content is a main factor influencing the silage fermentation of the peanut seedlings.
(III) analysis of nutritive value of silage peanut seedlings
Taking 500 g of fresh samples of the silage peanut seedlings obtained by each group as samples to be detected by a quartering method, taking four samples of each group, drying the samples in a 65 ℃ oven for 48 hours, and detecting initial moisture after full moisture regain. Crushing the dried sample by a plant micro crusher, sieving the crushed sample by a 1 mm analysis sieve, and detecting related indexes according to the following method:
the content of Crude Protein (CP), Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) of the samples was determined according to the feed analysis and quality testing technique of St.X.3 (3 rd edition). Determination of soluble sugars (WSC): the results of the colorimetric determination using sulfuric acid-anthrone are shown in Table 2.
TABLE 2 nutritional value index (% of dry matter basis) of the peanut vine silage obtained from each test group
Note: those in the same column with different letters indicate significant differences (P < 0.05).
The results in table 2 show that the differences in dry matter content were not significant for each treatment group after 60 d fermentation (P > 0.05). The crude protein content of the M-L group and the M-EP-L group is obviously lower than that of the EP-L group (P < 0.05). The content of neutral detergent fiber and acidic detergent fiber in the control group is higher, and the content of neutral detergent fiber and acidic detergent fiber is obviously higher than that in the M-EP-L group (P < 0.05), which shows that the combined treatment of saccharomycete and enzyme has the best effect of degrading and utilizing fiber components in the whole peanut seedlings. The highest soluble sugar of the M-EP-L group is obviously higher than that of a control group (P is less than 0.05) because the lactic acid is fermented in a large quantity, the pH is reduced, the activity of harmful microorganisms such as putrefying bacteria, clostridium and the like is inhibited, the consumption of the soluble sugar by the self respiration of plants in the early stage of ensiling fermentation is reduced, nutrient substances are effectively reserved, and the cellulase can degrade structural polysaccharide of plant cell walls into monosaccharide, increase the content of soluble carbohydrate, provide sufficient substrate for microbial fermentation and improve the quality of ensiling feed.
The peanut vine silage method can promote fermentation of lactic acid bacteria in the peanut vine silage, and the pH value is obviously reduced; the content of lactic acid in organic acid of a treatment group containing 10g of molasses, 125U of cellulase, 25U of cellobiase, 375U of pectinase, 1.0 x 108cfu of lactobacillus plantarum and 3.0 x 107cfu of pediococcus acidilactici added into each kilogram of peanut seedling material is increased by 39 percent compared with natural silage, and neutral detergent fiber and acid detergent fiber are respectively reduced by 5.88 percent and 7.02 percent compared with natural silage, thereby improving the silage quality and the nutritional value of the silage peanut seedlings.
EXAMPLE II peanut vine silage test
Peanut seedling ensiling
1. Cutting: harvesting peanut seedlings with the moisture content of 65-75% (mass percentage content) in the peanut harvesting period, harvesting the peanut seedlings with the stubble height of 3-5 cm, and cutting the peanut seedlings into 3-5 cm grass sections by using a hay cutter; randomly dividing the cut peanut seedlings into five groups, namely a control group, an enzyme and bacterium treatment group, a sugar and enzyme treatment group, a sugar and bacterium treatment group and a sugar and enzyme and bacterium treatment group;
2. adding an additive: adding additives into the groups of cut peanut seedlings obtained in the step 1 according to the following modes:
control group (CK group): sterile water was added in an amount of 25 ml/kg, equal to the treatment group additive volume.
Enzyme + bacteria treatment group (EP-L group): adding lactobacillus additive and complex enzyme preparation into water to obtain mixed solution, and uniformly spraying the mixed solution on peanut seedling material to ensure that each kilogram of peanut seedling material corresponds to about 1.0 × 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici, 210U cellulase, 30U cellobiase and 450U pectinase.
Sugar + enzyme treatment group (M-EP group): adding molasses and complex enzyme preparation into water to obtain mixed solution, and homogenizingSpraying on peanut vine material to ensure that each kilogram of peanut vine material corresponds to about 7.5g of molasses and 1.0 x 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici.
Sugar + bacteria treatment group (M-L group): adding molasses and lactobacillus additives into water to obtain a mixed solution, and uniformly spraying the mixed solution on peanut vine materials to ensure that each kilogram of peanut vine materials corresponds to about 7.5g of molasses, 210U of cellulase, 30U of cellobiase and 450U of pectinase.
Sugar + enzyme + bacteria treatment group (M-EP-L group): adding molasses, a complex enzyme preparation and a lactic acid bacteria additive into water to obtain a mixed solution, uniformly spraying the mixed solution on peanut vine materials to ensure that each kilogram of peanut vine materials corresponds to about 7.5g of molasses, 210U of cellulase, 30U of cellobiase, 450U of pectinase and 1.0 multiplied by 108cfu Lactobacillus plantarum, 3.0X 107cfu Pediococcus acidilactici.
The amount of water added was equal for each group.
3. And (3) storage: the method of the first embodiment is carried out
(II) fermentation quality analysis of silage peanut seedlings
The procedure was followed as in example one. The results of the pH, organic acid content and ammoniacal nitrogen/total nitrogen measurements of the samples are shown in table 3.
TABLE 3 comparison of fermentation quality indexes of the silage for peanut seedlings obtained from each test group
Note: % DM represents the proportion of organic acids in dry matter; the letters in the same column indicate significant difference (P < 0.05), and the table is as follows; ND means not detected.
The results in Table 3 show that, compared with the single silage of peanut seedlings (control group), the combined addition of molasses and lactobacillus or/and the compound enzyme preparation can obviously reduce the pH value of the peanut silage, obviously increase the lactic acid content and obviously reduce the ammonia nitrogen/total nitrogen content, and the combined addition of the molasses and the lactobacillus or/and the compound enzyme preparation can obviously improve the fermentation quality of the peanut seedling silage
(III) analysis of nutritive value of silage peanut seedlings
The procedure was as in step three of example 1. The results of the measurement of Crude Protein (CP), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF) and soluble sugar (WSC) of the sample are shown in Table 4.
TABLE 4 nutritional value index (% dry matter basis) of the peanut vine silage obtained from each test group
Note: those in the same column with different letters indicate significant differences (P < 0.05).
The results in table 4 show that compared with the ensiling of the peanut seedlings alone (control), the ensiling of the peanut seedlings with the combination of molasses and lactic acid bacteria or/and the complex enzyme preparation has reduced content of neutral detergent fiber and acidic detergent fiber, increases the content of soluble carbohydrate, effectively retains nutrient substances, improves the palatability of the ensiling peanut seedlings, and improves the nutritional quality of the ensiling peanut seedlings.
The peanut vine silage method can promote fermentation of lactic acid bacteria in the peanut vine silage, and the pH value is obviously reduced; the organic acid added in each kilogram of peanut seedling material contains 7.5g of molasses, 150U of cellulase, 30U of cellobiase, 450U of pectinase, 1.0 x 108cfu of lactobacillus plantarum and 3.0 x 107cfu of pediococcus acidilactici, the lactic acid content of the organic acid is increased by 33.8 percent compared with natural silage, and neutral detergent fiber and acid detergent fiber are respectively reduced by 9.53 percent and 9.34 percent compared with natural silage, so that the silage quality and the nutritional value of the silage peanut seedlings are improved.
While the present invention has been described with reference to exemplary embodiments, it will be understood by those skilled in the art that various changes in the details of the above-described embodiments may be made, and equivalents of the related steps, methods and materials may be substituted for elements thereof without departing from the scope of the present invention, which is intended to encompass all such changes and modifications as fall within the true spirit and scope of the present invention.
Claims (9)
1. The peanut seedling silage additive is characterized by comprising 300-400 g/L of soluble sugar and 4 x 10 of lactic acid bacteria9～4×1010cfu/L or/and complex enzyme 2X 104～2.2×104 U/L。
2. The additive for ensiling peanut vine according to claim 1, wherein the lactic acid bacteria are derived from a lactic acid bacteria agent produced by Raman animal Nutrition Limited, and the lactic acid bacteria agent contains Lactobacillus plantarum at least 5.0 x 1010cfu/g, Pediococcus acidilactici ≥ 1.5 × 1010 cfu/g。
3. The peanut seedling silage additive of claim 1, wherein the complex enzyme is derived from a complex enzyme preparation, the complex enzyme preparation is composed of neutral cellulase, cellobiase and pectinase according to a volume ratio of 2:1:1, and the enzyme activity is as follows: the neutral cellulase is more than or equal to 2500U/mL, the cellobiase is more than or equal to 1000U/mL, and the pectinase is more than or equal to 15000U/mL.
4. The additive for ensiling peanut vine according to claim 1, wherein the soluble sugar is at least one of molasses, glucose, starch and sucrose.
5. A peanut seedling ensiling method comprises the following steps:
(1) cutting the harvested fresh peanut seedlings into grass fragments for later use;
(2) spraying the peanut vine silage additive of claim 1 onto the peanut vine material obtained in the previous step;
(3) and (3) carrying out vacuum packaging and sealing on the material obtained by the treatment in the step (2), and ensiling for 35-65 days in a room-temperature and dark environment.
6. A method for ensiling peanut vine according to claim 5, wherein in the step (2), the dosage standard of the additive for ensiling peanut vine is as follows:
so thatEach kg of peanut vine material is correspondingly provided with 7.5-10 g of soluble sugar and more than or equal to 1.3 multiplied by 10 viable count of lactobacillus8500-650U of cfu or/and complex enzyme; alternatively, the first and second electrodes may be,
adding 100-400 ml/kg fresh peanut vine of peanut vine silage additive.
7. A peanut vine silage method according to claim 5, characterized in that in step (2), the moisture content of the material is adjusted to 60% -75% after the peanut vine silage enzyme preparation is sprayed.
8. A peanut vine ensiling method according to claim 5, wherein in the step (1), the stubble height of the fresh peanut vine when being harvested is 3-5 cm.
9. The peanut vine ensiling method according to claim 5, wherein in the step (1), the harvested fresh peanut vine is cut into 3-5 cm grass segments by a hay cutter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110414765.XA CN112998139A (en) | 2021-04-17 | 2021-04-17 | Additive for ensiling peanut seedlings and ensiling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110414765.XA CN112998139A (en) | 2021-04-17 | 2021-04-17 | Additive for ensiling peanut seedlings and ensiling method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112998139A true CN112998139A (en) | 2021-06-22 |
Family
ID=76388584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110414765.XA Pending CN112998139A (en) | 2021-04-17 | 2021-04-17 | Additive for ensiling peanut seedlings and ensiling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112998139A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304398A (en) * | 2022-01-14 | 2022-04-12 | 江西省农业技术推广中心 | Fresh peanut vine mechanical silage preservation method and silage thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946872A (en) * | 2010-08-31 | 2011-01-19 | 广西皇氏甲天下乳业股份有限公司 | Silage EM (Effective Microorganisms) microbial agent added with enzyme and trace elements and preparation method thereof |
| CN105454668A (en) * | 2015-11-20 | 2016-04-06 | 南宁学院 | Silage method of peanut vine |
| CN106376747A (en) * | 2016-09-05 | 2017-02-08 | 中山市康泰生物科技有限公司 | Pregnant sow micro-storage fermentation feed and preparation method thereof |
-
2021
- 2021-04-17 CN CN202110414765.XA patent/CN112998139A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946872A (en) * | 2010-08-31 | 2011-01-19 | 广西皇氏甲天下乳业股份有限公司 | Silage EM (Effective Microorganisms) microbial agent added with enzyme and trace elements and preparation method thereof |
| CN105454668A (en) * | 2015-11-20 | 2016-04-06 | 南宁学院 | Silage method of peanut vine |
| CN106376747A (en) * | 2016-09-05 | 2017-02-08 | 中山市康泰生物科技有限公司 | Pregnant sow micro-storage fermentation feed and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304398A (en) * | 2022-01-14 | 2022-04-12 | 江西省农业技术推广中心 | Fresh peanut vine mechanical silage preservation method and silage thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112890004B (en) | Whole cassava plant biological fermentation feed and preparation method thereof | |
| CN113273645B (en) | Silage method of mulberry green feed | |
| CN103652508A (en) | Ensilage combination including barley straws and festuca arundinacea and application thereof | |
| CN114027404A (en) | Silage additive for corn silage and application thereof | |
| CN109965113A (en) | A kind of ensiling paper mulberry feed and its preparation method and application | |
| CN108575885A (en) | A kind of black swine rearing method | |
| CN105901308A (en) | Making method of ramie and corn flour mixed silage | |
| CN107912604A (en) | A kind of silage composition and preparation method | |
| CN104543335A (en) | Biological fermentation feed containing pleurotus eryngii waste mushroom bran ingredients and used for raising fat meat donkey as well as application of biological fermentation feed | |
| CN112998139A (en) | Additive for ensiling peanut seedlings and ensiling method | |
| CN105166412A (en) | Compound microbial preparation and application thereof | |
| Tao et al. | Fermentation quality and aerobic stability of Napier grass ensiled with citric acid residue and lactic acid bacteria | |
| CN111387354A (en) | Silage and preparation method and application thereof | |
| CN106538849A (en) | A kind of compoiste fermented microbial inoculum of millet straw and its ensiling millet straw feedstuff and method of preparation | |
| CN114190489B (en) | Preparation method of microbial fermentation chicken feed based on hybrid broussonetia papyrifera and mugwort mixture | |
| CN107912606A (en) | A kind of hay silage feed containing agricultural crop straw and preparation method thereof | |
| CN115462447A (en) | Method for preparing sorghum straw whole-plant corn mixed storage forage grass | |
| CN109452479A (en) | A kind of method that high-moisture corn fermentation generates people's pannage | |
| Morm et al. | Gas Kinetics, Rumen Characteristics, and In Vitro Degradability of Varied Levels of Dried and Fresh Cassava Leaf Top Fermented with Cassava Pulp | |
| Sainz-Ramírez et al. | Effect of the cutting date and the use of additives on the chemical composition and fermentative quality of sunflower silage | |
| CN112335778A (en) | Fermented sweet sorghum TMR feed and preparation method thereof | |
| CN111184135A (en) | Suaeda salsa pellet feed and application thereof in sheep breeding | |
| Khanday et al. | A review on fermentation quality of paddy straw silage | |
| CN112369502A (en) | Tail vegetable fermented feed for livestock and poultry and preparation method thereof | |
| CN115176886B (en) | Silage for high-protein low-fiber hybrid broussonetia papyrifera and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |