CN112980665A

CN112980665A - Mycoplasma detector

Info

Publication number: CN112980665A
Application number: CN201911272838.5A
Authority: CN
Inventors: 朱江烨
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-12
Filing date: 2019-12-12
Publication date: 2021-06-18

Abstract

The invention discloses a mycoplasma detector, which comprises a main controller and a sample detection incubation device, wherein the sample detection incubation device comprises an incubation plate, a heating plate arranged at the bottom side of the incubation plate, and a photoelectric plate and a photoelectric detection plate which are arranged at two opposite sides of the incubation plate; the heating plate is provided with a temperature sensor, the photoelectric plate is provided with an LED light source, the photoelectric detection plate is provided with a color sensor, and the incubation plate is provided with a sample test tube placing hole and a corresponding detection hole; the heating plate heats the incubation plate; the photoelectric plate emits light rays which pass through the detection holes and the sample test tubes placed in the sample test tube placing holes and irradiate the photoelectric detection plate. The temperature of the test tube in the test tube placing hole is controlled to be constant by utilizing the heating plate and the temperature sensor; the photoelectric detection plate and the color sensor are used for identifying the change of color and judging the detection result by utilizing the light transmission in the photoelectric plate to pass through the detection hole and the sample reagent bottle, so that the error is reduced compared with the error identified by naked eyes, and the efficiency is greatly improved compared with the manual operation.

Description

Mycoplasma detector

Technical Field

The invention belongs to the technical field of medical detection instruments, relates to a detector, and particularly relates to a mycoplasma pneumoniae detector.

Background

Mycoplasma pneumoniae is a microbe that is intermediate between viruses and bacteria and is the causative agent of mycoplasma pneumoniae in humans. The pathogenicity of mycoplasma pneumoniae may be related to allergic reactions of infected persons to pathogens or their metabolites. After being inhaled by respiratory tract, the mycoplasma pneumoniae is adhered to the surface of host respiratory tract epithelial cells to inhibit ciliary movement and destroy the epithelial cells. The diagnosis of mycoplasma pneumoniae in China mainly depends on serological detection and pathogen culture.

At present, pathogen culture methods are gradually adopted at home and abroad to diagnose mycoplasma pneumoniae, and a 3 h/6 h rapid detection kit is formed. The published literature is, for example, Chinese patent application with application number CN201410118844, which discloses Mycoplasma pneumoniae culture identification reagent and a preparation method thereof (publication number CN 103849673A). compared with the traditional detection method, the rapid Mycoplasma pneumoniae detection kit adopted by the method is greatly improved, for example, the operation is more convenient, the detection is more rapid, and the like. However, the existing kits are all detected manually, so that human operation errors such as uneven sample application, improper detection reagent control and the like are inevitable. In addition, the detection result of the enzymatic chromogenic reaction has larger deviation through manual visual interpretation. Therefore, the manual detection efficiency is too low, and the interpretation result is inaccurate.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the mycoplasma pneumoniae detector which has high operation efficiency and reduces operation errors, and is used for replacing manual operations of incubation, printing and result judgment when a medical institution adopts a mycoplasma pneumoniae rapid detection reagent to check mycoplasma pneumoniae.

The purpose of the invention can be realized by the following technical scheme:

the mycoplasma detector comprises a main controller, a sample detection incubation device, a display screen, a printer and an external shell for bearing the components, wherein the sample detection incubation device comprises an incubation plate, a heating plate arranged at the bottom side of the incubation plate, and a photoelectric plate and a photoelectric detection plate which are arranged at two opposite sides of the incubation plate; the heating plate is provided with a temperature sensor, the photoelectric plate is provided with an LED light source, the photoelectric detection plate is provided with a color sensor, and the incubation plate is provided with a sample test tube placing hole and a corresponding detection hole; the heating plate heats the incubation plate; the photoelectric plate emits light rays which pass through the detection holes and the sample test tubes placed in the sample test tube placing holes and irradiate the photoelectric detection plate.

The heating plate, the temperature sensor, the photoelectric plate and the photoelectric detection plate are all electrically connected to the main controller.

The board of hatching be equipped with three groups, every group is hatched the board and is equipped with five sample test tube pendulum holes, the both sides that every group is hatched the board and is corresponded are equipped with the inspection hole.

The external shell is formed by assembling a base and a shell, a display screen mounting hole, a printer mounting hole and three groups of incubation plate holes are formed in the shell, the incubation plate holes are suitable for being provided with hole covers, and the hole covers are rotatably connected with the shell.

A main controller is arranged on the base; the display screen mounting hole and the printer mounting hole are correspondingly provided with the display screen and the printer; the display screen and the printer are respectively electrically connected to the main controller; the incubation plate holes are correspondingly provided with incubation plates.

The shell at the position of the incubation plate hole is marked with a number and a reagent bottle placing direction mark.

The main controller reads the value of the temperature sensor, analyzes and calculates the value of the temperature sensor and the set temperature value input by the user through the touch display screen, and controls the heating function of the heating plate.

The main controller is electrically connected with the control end of the relay, and the switch end of the relay is respectively electrically connected with the heating plate power supply and the heating plate.

The main controller sends an opening control signal to the LED light source on the photoelectric plate, the LED light source is lightened, and light rays pass through the sample reagent bottle and then reach the photoelectric detection plate; after the main control reads the numerical value of the color sensor of the corresponding channel of the photoelectric detection plate, the main control sends a closing control signal to the LED light source of the channel on the photoelectric plate, and the LED light source is closed.

The main controller is electrically connected with the display screen, parameter information, sample information and patient information data of the instrument are input into the main controller through the display screen, and the main controller displays data such as detection results and instrument working states through the display screen.

The invention has the beneficial effects that: the temperature of the test tube in the test tube placing hole is controlled to be constant by utilizing the heating plate and the temperature sensor; the photoelectric detection plate and the color sensor are used for identifying the change of color and judging the detection result by utilizing the light transmission in the photoelectric plate to pass through the detection hole and the sample reagent bottle, so that the error is reduced compared with the error identified by naked eyes, and the efficiency is greatly improved compared with the manual operation.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic view of the housing construction of the present invention;

FIG. 2 is a schematic view of the base structure of the present invention;

FIG. 3 is a schematic top view of an incubation plate of the present invention;

FIG. 4 is a schematic cross-sectional view of an incubation plate A-A according to the present invention;

FIG. 5 is a schematic bottom view of an incubation plate of the present invention;

FIG. 6 is a schematic side view of an incubation plate of the present invention;

FIG. 7 is a schematic side view of the other side of the inventive plate;

fig. 8 is a schematic view of the connection of components of the present invention.

Detailed Description

The mycoplasma detector comprises a main controller, a sample detection incubation device, a display screen, a printer and an external shell for bearing the components;

as shown in fig. 1 and 2, the external casing is formed by assembling a base 1 and a casing 2, the casing 2 is provided with a display screen mounting hole 3, a printer mounting hole 4 and three groups of incubation plate holes 5, the incubation plate holes 5 are adapted with hole covers, the hole covers are rotatably connected with the casing 2, and the display screen and the printer are correspondingly mounted at the positions of the display screen mounting hole 3 and the printer mounting hole 4;

the main controller is fixed on the base 1, and the display screen and the printer are connected to the main controller through wires, see fig. 8;

referring to fig. 3 to 7, the sample detection incubation device includes three groups of incubation plates 6, a heating plate 9 respectively installed at the bottom side of each group of incubation plates, and a photoelectric plate 11 and a photoelectric detection plate 12 installed at opposite sides of the incubation plates, a temperature sensor 10 is installed on the heating plate 9, an LED bulb is provided on the photoelectric plate 11, a color sensor is provided on the photoelectric detection plate 12, and the heating plate, the temperature sensor, the photoelectric plate and the photoelectric detection plate are all connected to a main controller through wires, see fig. 8;

the three groups of incubation plates 6 are correspondingly arranged on the shell at the positions of the incubation plate holes 5, each group of incubation plates is provided with five sample test tube placing holes 7, and two corresponding sides of each group of incubation plates are provided with detection holes 8;

the photoelectric plates 11 corresponding to the two sides of the sample test tube placing hole 7 correspond to the photoelectric detection plate 12 and the position of each detection hole, the color sensor identifies the color change of a sample reagent reactant in the sample test tube placing hole 7, transmits a judgment result to the main controller, judges a detection result according to the color change and displays the detection result on the display screen;

the hot plate 9 that the bottom side corresponds has guaranteed sample test tube pendulum hole constancy of temperature, and the hot plate adopts the resistance wire on the printed circuit board to heat, and the start-up and the stopping of main control unit control hot plate, temperature sensor send the test tube temperature on putting the device for main control unit in real time.

Photoelectric plate 11 is located the well lower part position in sample test tube pendulum hole to make light can pass the well lower part position of sample test tube, the light of 11LED bulbs of photoelectric plate incides to the inspection hole with certain angle in, and transmit into sample reagent bottle, photoelectric detection board is in sample reagent bottle position behind, the last color sensor of photoelectric detection board is prior art, thereby can discern the colour change after the reaction and judge the testing result and send the testing result into the display screen through internal control ware.

The printer electrically connected with the main controller is convenient for operators to print the display result.

The shell at the position of the incubation plate hole 5 is marked with a number and a reagent bottle placing direction mark, and when a result is displayed, the number corresponding to the detection result can be displayed.

The invention has high operation efficiency and reduced operation error, the sample reagent test tube is placed in the test tube placing hole, the constant temperature is kept for a certain time (about 37 ℃) by using the heating plate, and the color change is identified by the color sensor after the reaction is finished and the result is judged.

The main controller reads the numerical value of the temperature sensor in real time through a one-wire protocol, the numerical value of the temperature sensor is the temperature value of the current incubation plate, the main controller analyzes and calculates the numerical value of the temperature sensor and the set temperature value input by a user through a touch display screen, if the numerical value acquired by the current temperature sensor is larger than the set value of the user, the main controller closes the heating function of the heating plate, and if the numerical value acquired by the current temperature sensor is smaller than the set value of the user, the main controller opens the heating function of the heating plate.

The main controller is electrically connected with the control end of the relay, and the switch end of the relay is respectively electrically connected with the heating plate power supply and the heating plate. When main control unit judged that the heating function need be opened through temperature analysis, main control unit sent switch-on signal for the relay, and the switch end of relay is closed, and the hot plate power passes through relay switch end intercommunication with the hot plate, and the hot plate begins the heating. When main control needs to close the heating function through temperature analysis judgement, main control unit sends off turn-off signal for the relay, and the switch end disconnection of relay, hot plate power and hot plate pass through relay switch end disconnection, and the hot plate stops the heating.

When the incubation of a sample in a certain detection channel is finished, the main controller sends an opening control signal to the LED light source of the channel on the photoelectric plate, the LED light source is lightened, and light rays pass through the sample reagent bottle and then reach the photoelectric detection plate. After the main control reads the numerical value of the color sensor of the corresponding channel of the photoelectric detection plate, the main control sends a closing control signal to the LED light source of the channel on the photoelectric plate, and the LED light source is closed.

After the incubation of the sample of a certain detection channel is finished, the main controller reads the numerical value of the color sensor of the corresponding channel on the photoelectric detection plate, and after the reading is finished, the main controller sends a control signal for turning off the LED light source to the photoelectric plate.

The main controller is electrically connected with the touch display screen, and data such as parameter information, sample information, patient information and the like of the instrument are input into the main controller through the touch display screen. The main controller displays the detection result, the working state of the instrument and other data through the display screen.

The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.

Claims

1. Mycoplasma detector, including main control unit, sample detection incubation device, display screen, printer and bear the outside casing of above-mentioned part, its characterized in that: the sample detection incubation device comprises an incubation plate (6), a heating plate (9) arranged at the bottom side of the incubation plate, and a photoelectric plate (11) and a photoelectric detection plate (12) which are arranged at two opposite sides of the incubation plate; a temperature sensor (10) is arranged on the heating plate (9), an LED light source is arranged on the photoelectric plate (11), a color sensor is arranged on the photoelectric detection plate (12), and a sample test tube placing hole (7) and a corresponding detection hole (8) are arranged on the incubation plate (6); the heating plate (9) heats the incubation plate (6); the photoelectric plate (11) emits light rays which pass through the detection hole (8) and the sample test tube placed in the sample test tube placing hole (7) and irradiate the photoelectric detection plate (12).

2. A Mycoplasma detector according to claim 1, wherein the heating plate, the temperature sensor, the photoelectric plate, and the photoelectric detection plate are all electrically connected to a main controller.

3. The mycoplasma detector according to claim 1, wherein the incubation plates (6) are provided with three groups, each group of incubation plates is provided with five sample tube placing holes (7), and each group of incubation plates is provided with detection holes (8) on two corresponding sides.

4. The mycoplasma detector according to claim 1, wherein the outer casing is assembled by a base (1) and a casing (2), the casing (2) is provided with a display screen mounting hole (3), a printer mounting hole (4) and three groups of incubation plate holes (5), the incubation plate holes (5) are adapted with hole covers, and the hole covers are rotatably connected with the casing (2).

5. The mycoplasma detector according to claim 4, wherein the base (1) is provided with a main controller; the display screen mounting hole (3) and the printer mounting hole (4) are correspondingly provided with a display screen and a printer; the display screen and the printer are respectively electrically connected to the main controller; the incubation plate holes (5) are correspondingly provided with incubation plates (6).

6. The mycoplasma detector according to claim 4, wherein the casing at the position of the incubation plate hole (5) is marked with a number and a reagent bottle placement direction mark.

7. The mycoplasma detector according to claim 1, wherein the main controller reads the value of the temperature sensor, analyzes and calculates the value of the temperature sensor and the set temperature value input by the user through the touch display screen, and controls the heating function of the heating plate.

8. A Mycoplasma detector according to claim 7, wherein the main controller is electrically connected to the control terminal of the relay, and the switch terminals of the relay are electrically connected to the hot plate power supply and the hot plate, respectively.

9. The mycoplasma detector according to claim 1, wherein the main controller sends an on control signal to the LED light source on the photoelectric plate, the LED light source is lighted, and light passes through the sample reagent bottle and reaches the photoelectric plate; after the main control reads the numerical value of the color sensor of the corresponding channel of the photoelectric detection plate, the main control sends a closing control signal to the LED light source of the channel on the photoelectric plate, and the LED light source is closed.

10. The mycoplasma detector according to claim 1, wherein the main controller is electrically connected to the display screen, the parameter information, sample information, and patient information data of the mycoplasma detector are inputted into the main controller via the display screen, and the main controller displays the detection result, the working status of the mycoplasma detector, and other data via the display screen.