CN112972628A

CN112972628A - Traditional Chinese medicine composition, preparation thereof, preparation method and application thereof

Info

Publication number: CN112972628A
Application number: CN202110463888.2A
Authority: CN
Inventors: 张铁军; 刘昌孝; 龚苏晓; 郑国华; 徐旭; 李新; 杨冰
Original assignee: Tianjin Institute of Pharmaceutical Research Co Ltd
Current assignee: Tianjin Institute of Pharmaceutical Research Co Ltd
Priority date: 2021-04-28
Filing date: 2021-04-28
Publication date: 2021-06-18

Abstract

The invention belongs to the field of medicines, and discloses a traditional Chinese medicine composition, a preparation thereof, a preparation method and application thereof. The Chinese medicinal composition is prepared from herba Lysimachiae Foenumgraeci, herba Eupatorii, bitter orange flower, folium Artemisiae Argyi, radix Angelicae Dahuricae, flos Lonicerae, herba Agastaches, and fructus Tsaoko, and can be used for inhibiting pathogenic microorganisms such as bacteria and virus in body surface, article and air, and treating bacterial pneumonia. The preparation method is simple, the cost is low, the quality is controllable, no chemical synthetic spice is contained, the environment is not polluted, and the method is suitable for industrial mass production and convenient to use.

Description

Traditional Chinese medicine composition, preparation thereof, preparation method and application thereof

Technical Field

The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine composition, a preparation thereof, a preparation method and application thereof.

Background

Pathogenic microorganisms refer to microorganisms that can invade the human body, causing infection and even infectious diseases, among which the harmfulness is greatest with bacteria and viruses. Pathogenic microorganisms exist and spread in the air mainly in three forms: (1) attached to dust; (2) attached to droplets of spray from the nasal or oral cavity; (3) attached to the 'spray core' formed after the spray surface is evaporated. If a person breathes, contacts or inhales these pathogenic microorganisms, a disease can be triggered. The greater the bacterial virus density in the local space, the more pathogenic. Common airborne diseases include pneumonia, tuberculosis, influenza, and the like. As with the new coronavirus pneumonia that is currently outbreaking worldwide, its main clinical manifestations are respiratory symptoms.

The most direct and effective means for the prevention and treatment of bacterial and viral diseases: firstly, the bacteria and viruses in the external environment are killed or inhibited, and secondly, the capability of resisting the infection of the bacteria and the viruses in the human body is enhanced.

In 2003, after the outbreak of the infectious atypical pneumonia, the applicant of the patent successfully applied for a patent of 'a pure traditional Chinese medicine antiviral and bactericidal spray and a preparation method thereof' (patent number ZL 03130584.9), on the basis, according to the characteristics of COVID-19 epidemic situation, the invention is developed according to reasonable formula with the purposes of in vivo and in vitro antibiosis and antivirus, and the capability of improving the body defense is achieved according to the rules of regulating qi and the like.

The product is prepared from holy basil, eupatorium, seville orange flower, folium artemisiae argyi, radix angelicae, honeysuckle, patchouli, tsaoko amomum fruit and other traditional Chinese medicines, can be used for aromatizing, eliminating dampness, dispersing exterior evil, resolving turbidity, removing dirt, and regulating qi activity, can improve the external environment of a living space, can enter the body through respiratory tract inhalation or oral administration, improves the internal environment of the body, and achieves the effects of eliminating evil and strengthening body resistance. The honeysuckle flower in the formula has the effects of clearing away heat and toxic materials and dispelling wind and heat; herba Agastaches has effects of eliminating turbid pathogen with aromatics, stimulating appetite, relieving vomit, relieving exterior syndrome and relieving summer-heat; the eupatorium has the effects of aromatizing, eliminating dampness, activating spleen, stimulating appetite, relieving exterior syndrome and relieving summer heat; the radix Angelicae Dahuricae has antipyretic, analgesic, antibacterial, antiinflammatory, and blood vessel dilating effects; fructus Tsaoko has effects of eliminating dampness, warming middle energizer, preventing malaria, and eliminating phlegm; the seville orange flower has the functions of antibiosis, antivirus, anti-inflammation, gastrointestinal function improvement and the like; in addition to antibacterial, antiviral, antiinflammatory and analgesic effects, folium Artemisiae Argyi has antitussive, antiasthmatic, and immunity enhancing effects.

Disclosure of Invention

The invention provides a traditional Chinese medicine composition and a preparation thereof, and provides a preparation method and application thereof in order to solve the technical problems. The traditional Chinese medicine composition can effectively inhibit pathogenic microorganisms such as bacteria, viruses and the like on the body surface, articles and in the air in vitro, can effectively prevent and treat bacterial pneumonia in vivo, is easy for industrial production and is convenient for long-term daily use.

The invention aims to provide a pure traditional Chinese medicine composition which is prepared by extracting the following raw materials in parts by weight: 50-150 parts of holy basil, 50-150 parts of eupatorium fortunei, 50-150 parts of seville orange flower, 50-150 parts of folium artemisiae argyi, 10-100 parts of radix angelicae, 10-100 parts of honeysuckle, 10-100 parts of pogostemon cablin and 10-100 parts of amomum tsao-ko.

Preferably: 80-120 parts of holy basil, 80-120 parts of eupatorium fortunei, 80-120 parts of seville orange flower, 80-120 parts of folium artemisiae argyi, 10-50 parts of radix angelicae, 10-50 parts of honeysuckle, 10-50 parts of pogostemon cablin and 10-50 parts of amomum tsao-ko.

Most preferably: 100 parts of holy basil, 100 parts of eupatorium, 100 parts of seville orange flower, 100 parts of folium artemisiae argyi, 25 parts of radix angelicae, 25 parts of honeysuckle, 25 parts of pogostemon cablin and 10 parts of amomum tsao-ko.

The traditional Chinese medicine composition is prepared into active substances by extracting the raw materials composed of the formula and other modes, and the active substances are taken as raw materials to prepare any pharmaceutically acceptable preparation formulation according to the conventional preparation technology. The active substance can be obtained by separate pulverization or extraction, or by co-pulverization or extraction, such as: the active substances can be powder or extract substances, dry extract or fluid extract, and can be prepared into different concentrations according to different requirements of preparations.

The traditional Chinese medicine composition can be prepared into any pharmaceutically acceptable dosage form. These dosage forms are selected from: tablet, capsule, granule, pill, powder, oral liquid, paste, suspension, solution, spray, patch, etc. Preferred are oral solid or liquid formulations. The liquid preparation is preferably spray or oral liquid preparation. Most preferred is a spray.

The oral liquid preparation comprises the traditional Chinese medicine composition, one or more of a common polar solvent, a common semi-polar solvent or a common non-polar solvent, and one or more of a common flavoring agent, a common coloring agent and a common preservative.

The spray comprises the traditional Chinese medicine composition, medicinal ethanol and a surfactant. The surfactant is any one or more of an anionic surfactant, a cationic surfactant or a zwitterionic surfactant; the surfactant is preferably polyoxyethylene 40 hydrogenated castor oil; the adding amount of the surfactant is 1 to 10 percent of the weight of the medicinal materials, and the preferable amount is 2 to 6 percent; the alcohol content is 10-75%, preferably 10-40%, more preferably 20-40%; the content of the medicinal materials is 2 to 20 percent, preferably 2 to 10 percent, and more preferably 3 to 6 percent.

The preparation method of the traditional Chinese medicine composition comprises the following steps:

taking medicinal materials, adding water with the weight 8-20 times of that of the medicinal materials, extracting for 1-3 times by a steam distillation method, each time for 1-4 hours, collecting volatile oil, combining extracting solutions, standing, filtering, concentrating under reduced pressure, drying, and mixing with the volatile oil to obtain a traditional Chinese medicine composition;

or taking the medicinal materials, adding water which is 8-20 times the weight of the medicinal materials, extracting for 1-3 times by adopting a steam distillation method, collecting volatile oil each time for 1-4 hours, combining the extracting solutions, concentrating the extracting solution to 1.05-1.20, adding 70-95% ethanol, precipitating to 50-70%, standing for 6-24 hours, concentrating and drying supernate, and mixing with the volatile oil to obtain the traditional Chinese medicine composition;

or taking the medicinal materials, adding 6-12 times of 30-95% ethanol, heating and refluxing for 1-4 times, 1-3 hours each time, collecting volatile oil, combining the extracting solutions, concentrating under reduced pressure, drying, and mixing with the volatile oil to obtain the traditional Chinese medicine composition;

or taking the medicinal materials, adding 6-20 times of 30-95% ethanol, percolating or cold-soaking for 1-4 times, combining the extracting solutions, concentrating under reduced pressure, and drying to obtain a traditional Chinese medicine composition;

the percolation speed is 1-5 ml/min until no percolate flows out;

the cold soaking extraction time is 6-24 hours each time.

The preferable preparation method of the traditional Chinese medicine composition comprises the following steps:

extracting the medicinal materials with 14 times of water by steam distillation for 2 times, 4 hr for the first time and 1 hr for the second time, collecting volatile oil, mixing extractive solutions, standing, filtering, concentrating under reduced pressure, drying, and mixing with volatile oil to obtain Chinese medicinal composition;

or taking the medicinal materials, adding water with the weight 14 times of the medicinal materials, extracting for 2 times by adopting a steam distillation method, extracting for 4 hours for the first time and 1 hour for the second time, collecting volatile oil, combining extracting solutions, concentrating the extracting solution to 1.15-1.20, adding 95% ethanol, precipitating to 60%, standing for 12 hours, taking supernate, concentrating, drying, and mixing with the volatile oil to obtain a traditional Chinese medicine composition;

or pulverizing the above materials into coarse powder, percolating, adding 70% ethanol 15 times at 3ml/min until no percolate flows out; collecting percolate, concentrating under reduced pressure, and drying to obtain Chinese medicinal composition.

The preparation method of the spray comprises the following steps:

mixing the traditional Chinese medicines according to the weight ratio, extracting for 1-4 times by using 0-95% ethanol, wherein the dosage of a solvent is 5-20 times of the weight of the traditional Chinese medicines, filtering, combining filtrates, adding a surfactant with the dosage of 1-10% of the weight of the traditional Chinese medicines, dissolving and uniformly mixing; adding water to adjust the alcohol content of the liquid medicine to 10-75 percent and the medicine content to 2-20 percent;

the extraction method is any one of cold soaking, percolation, reflux and ultrasound.

Preferably, the preparation method of the spray comprises the following steps:

mixing the traditional Chinese medicines according to the weight ratio, percolating or cold-soaking the traditional Chinese medicines for 1-4 times by using 0-95% ethanol, filtering, combining the filtrates, adding 1-10% surfactant according to the weight of the traditional Chinese medicines, dissolving and uniformly mixing the filtrates, wherein the dosage of the solvent is 5-20 times of the weight of the traditional Chinese medicines; adding water to adjust the alcohol content of the liquid medicine to 10-40 percent and the medicine content to 2-10 percent;

the percolation speed is 1-5 ml/min until no percolate flows out;

the cold leaching extraction time is 6-72 h.

Further preferably, the preparation method of the spray comprises the following steps:

mixing the traditional Chinese medicines according to the weight ratio, percolating or cold-soaking the traditional Chinese medicines for 1-3 times by using 50-80% ethanol, filtering, combining the filtrates, adding 2-6% surfactant according to the weight of the traditional Chinese medicines, dissolving and uniformly mixing the filtrates, wherein the dosage of the solvent is 8-15 times of the weight of the traditional Chinese medicines; adding water to adjust the alcohol content of the liquid medicine to be 20-40 percent and the medicine content to be 3-6 percent;

the percolation speed is 2-4 ml/min until no percolate flows out;

the first cold leaching extraction time is 24-48 hours, and the later cold leaching extraction time is 6-12 hours.

The invention adopts holy basil, eupatorium fortunei, seville orange flower, folium artemisiae argyi, angelica dahurica, honeysuckle, patchouli and amomum tsao-ko to clear away heat and toxic material and disperse wind and heat, and has the functions of antibiosis and antivirus. Wherein:

the herba Lysimachiae foenumgraeci is dried whole plant of Lysimachia foenum-graecum Hance of Primulaceae. Dispel wind, relieve exterior syndrome, promote qi circulation and alleviate pain. Can be used for treating headache, nasal obstruction, chest distress, abdominal distention, and toothache. Menthol as an active ingredient has antipruritic, analgesic, refreshing and anti-inflammatory effects; the active ingredient beta-caryophyllene has bitter taste, has the functions of relieving asthma, relieving cough and eliminating phlegm, and is clinically used for treating tracheitis.

Eupatorium fortunei is the dried aerial part of Eupatorium fortunei Turcz. Fragrant, resolving dampness, activating spleen, stimulating appetite, relieving exterior syndrome and relieving summer-heat. Because the fragrance is like orchid, the herb of fortune's eupatorium can dispel summer-heat evil and avoid foul smell. Its functions can be summarized as relieving summer-heat, resolving dampness and removing filth. Because of its fragrant smell, it can open muscle and skin striae, penetrate hair orifice and dispel summer-heat and dampness; can repel foul, remove foul smell, activate spleen and resolve dampness. Therefore, it is indicated for the early stage of exogenous summer-heat-dampness and damp-warm disease. The volatile oil and flavonoid components of the eupatorium have certain bacteriostatic action, and have certain bacteriostatic action on bacillus subtilis, staphylococcus aureus, escherichia coli and tetracoccus. Compared with ultraviolet lamp disinfection and low-temperature plasma disinfection, the air is disinfected by using the freesia and eupatorium fumigation in a ward, the disinfection effect is basically consistent, the personnel flow is not limited during the operation, and the method is non-toxic and harmless to the human body and can be widely applied.

The substitute flower is dry bud of Citrus aurantium L.var.amara Engl. Regulate qi-flowing, relieve chest stuffiness, stimulate appetite. The limonene and other components in the seville orange flower volatile oil inhibit skin pathogenic bacteria and mold such as trichophyton rubrum, and also inhibit bacteria such as candida albicans, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus and the like. The substitutional flower essential oil also has an inhibiting effect on gram-negative bacteria and gram-positive bacteria, and meanwhile, substitutional flower flavone also has a good inhibiting effect on gram-negative bacteria. The flavonoid hesperidin and naringin in the seville orange flower have anti-inflammatory effect, and can improve formaldehyde ankle edema of mice. The seville orange flower volatile oil can obviously inhibit the proliferation activity of 3T3-L1 preadipocytes, thereby playing an anti-inflammatory role. The seville orange flower polyphenol can play an anti-inflammatory role by inhibiting the synthesis of IL-6, TNF-alpha and IL-1 beta.

The folium Artemisiae Argyi is dried leaf of Artemisia argyi L.et Vant. Warming channels to stop bleeding, dispelling cold and relieving pain; externally used for eliminating dampness and relieving itching. The folium artemisiae argyi plays a remarkable role in sterilizing staphylococcus aureus, escherichia coli, bacillus subtilis, candida albicans and aspergillus niger. The oleum folium Artemisiae Argyi has effects of relieving asthma, eliminating phlegm, and relieving cough, and contains terpinenol as main ingredient for generating drug effect, which can directly relax guinea pig trachea smooth muscle and resist tracheal contraction induced by barium chloride, acetylcholine, and histamine. The folium artemisiae argyi volatile oil has a remarkable inhibiting effect on the swelling of mice with inflammation caused by xylene. The volatile oil of folium Artemisiae Argyi can improve the quality of mouse immune organ, and enhance the proliferation ability of lymphocyte and immunity of organism.

Radix Angelicae Dahuricae is dried root of Angelica dahurica (Fisch. ex Hoffm.) Benth.et hook.f. or Angelica dahurica (Fisch. ex Hoffm.) Benth.et hook.f. var.fortusana (Boiss.) Shann et Yuan of Umbelliferae. Relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, dredging nasal orifice, etc. The radix Angelicae Dahuricae extract has certain inhibiting effect on bacteria, mold and yeast, and the coumarin component has the best inhibiting effect on bacteria. The isoimperatorin in radix Angelicae Dahuricae has antibacterial effect on Mycobacterium tuberculosis. The isoimperatorin in radix Angelicae Dahuricae has antibacterial effect on Mycobacterium tuberculosis. The radix Angelicae Dahuricae volatile oil can activate endogenous analgesic mechanism to exert analgesic effect. The volatile oil of radix Angelicae Dahuricae has analgesic effect, and its mechanism is to regulate the content of monoamine neurotransmitter in vivo. The radix Angelicae Dahuricae extract has antiinflammatory activity.

The flos Lonicerae is dried bud or flower with initial bloom of Lonicera japonica Thunb. Sweet in flavor and cold in nature, it enters lung, heart and stomach meridians. Has effects in clearing away heat and toxic materials, and dispelling pathogenic wind and heat, and can be used for treating carbuncle, furuncle, pharyngitis, erysipelas, toxic heat, dysentery, wind-heat type common cold, epidemic febrile disease, and fever. The honeysuckle has certain inhibition effect on staphylococcus aureus, dysentery bacillus, vibrio cholerae, hemolytic streptococcus, typhoid bacillus, escherichia coli, typhoid bacillus and other pathogenic bacteria, and can inhibit pneumococcus, tubercle bacillus, shigella, meningococcus, pseudomonas aeruginosa and streptococcus mutans. Wherein isochlorogenic acid in chlorogenic acid compounds has the strongest antibacterial effect and is used as the main antibacterial component of honeysuckle. The honeysuckle also has the antiviral effect and can delay the pathological change of respiratory viruses to cells. Phenolic acids and flavonoids in the honeysuckle can effectively inhibit viruses, and chlorogenic acid has obvious inhibition effect on the most common and most main syncytial virus of respiratory tract and Coxsackie group B3 virus. Chlorogenic acid can enhance the function of the immune system of the organism from the whole level from the aspects of improving cellular immunity and humoral immunity of the organism, thereby enhancing the capability of the organism to resist microbial infection.

Herba Agastaches is dry aerial part of herba Agastaches Pogostemon cablin (Blanco) Benth. Has effects in eliminating dampness with aromatics, regulating the middle warmer, relieving vomit, relieving exterior syndrome, and dispelling summerheat. It is pungent, warm and fragrant, and can open muscle and skin striae, open hair orifices, dispel exterior pathogen, resolve dampness and repel foul. It is indicated for exogenous pathogenic factors, summer-heat and dampness, the beginning of damp-warm disease, and wind-induced malaria. The patchouli has the functions of resisting bacteria, viruses and inflammation and improving the gastrointestinal function, is a commonly used medicine for treating summer heat and dampness and is also an edible medicinal raw material. The patchouli aqueous extract and the volatile oil have certain inhibition effects on escherichia coli, bacillus subtilis, staphylococcus albus, tetracoccus, shigella, staphylococcus aureus and other bacteria, the inhibition effect on staphylococcus aureus is obviously stronger than that of enterobacteria, and the inhibition effect on the growth and the reproduction of most skin bacteria is achieved. Patchouli oil shows an anti-adenovirus effect by destroying the viral capsid protein Hexon gene and preventing viruses from adsorbing cells. The patchouli alcohol serving as an active ingredient can strongly inhibit the replication of H1N1 influenza virus in vitro, has the function of resisting the influenza virus in vivo, obviously improves the survival rate and survival time of mice, and obviously reduces pulmonary inflammation.

The fructus Tsaoko is dry mature fruit of Amomum tsao-ko Crevost et Lemaire of Zingiberaceae. Dry dampness and warm the middle energizer, check malaria and remove phlegm. Can be used for treating cold-dampness retention, abdominal distention and pain, distention and fullness, emesis, malaria, fever, pestilence and fever. The volatile oil of fructus Tsaoko has certain inhibiting effect on bacteria and fungi. Has inhibitory effect on Escherichia coli, Tetragenococcus and Bacillus subtilis. The tsaoko volatile oil has obvious inhibiting effect on mouse auricle swelling and mouse cotton ball granuloma caused by dimethylbenzene, active ingredients such as citral and alpha-pinene have phlegm removing, asthma relieving and bacteria inhibiting effects, and terpineol, geraniol, nerolidol and the like have obvious tranquilizing and antibacterial effects; the 1, 8-cineole has high content, and has antiinflammatory, antiviral, pathogenic wind expelling, and analgesic effects.

Huoxiang Xiang xing san can resolve dampness-turbidity, so in Ben Cao Zheng Yi (Zheng Yi of materia Medica) "it is fragrant but not strongly suspected, and warm but not mostly dry and strong, and can dispel damp-evil of haze; radix Angelicae Dahuricae is "white in color and pungent in flavor" in Ben Cao gang mu ", warm in nature and thick in qi, and helps rhizoma Atractylodis to eliminate dampness and turbid in body; caoguo is pungent and fragrant, and is called as "pungent-flavor-qi-male, wind-evil-removing and crouching" in Wen Yi Lun (epidemic febrile disease); folium Artemisiae Argyi, bitter and pungent in flavor and fragrant in nature and warm in nature, is called as "it can penetrate all meridians to remove all kinds of diseases" in the book materia medica origin, and is supplemented with herba Agastaches, fragrant, and epidemic-removing.

Therefore, the product is used by the traditional Chinese medicines together, and can avoid dirt and dampness, clear heat and dispel summer heat, inhibit bacteria and viruses and prevent related diseases. The invention dehumidifies according to the aromatic and regulates the functional activities of qi, according to the susceptible population, the etiology and pathogenesis characteristics and the intervention principle of the traditional Chinese medicine, after the medicinal materials are reasonably proportioned, the obtained formula and the mixture obtained by optimized extraction have the antibacterial and antiviral effects, has strong inhibiting effect on influenza virus, epidemic encephalitis virus, Coxsackie virus, etc., has inhibiting effect on bacteria such as staphylococcus aureus, staphylococcus albus, hemolytic streptococcus, etc., and fungi such as hirsutella schreberi, microsporum gypseum, candida albicans, etc., meanwhile, the pneumonia phenotype of a rat induced by Klebsiella pneumoniae can be obviously improved, the cytokine storm induced by hyperfimmunity can be reduced, the mechanism may be realized by inhibiting the activation of TLR 4/NF-kB signal channel, and can adjust the physical and psychological response under the stress state. Based on the above, the formula is suitable for preparing various preparations for inhibiting pathogenic microorganisms on body surfaces, articles and in the air or treating bacterial pneumonia. The extract can be prepared into spray according to the characteristics that pathogenic microorganisms can be transmitted by contact, air spray and even possible aerosol, and meanwhile, the aims of improving the body defense capacity and daily antibacterial disinfection are achieved, and the use is convenient.

Drawings

FIG. 1 is a graph showing the effect of the preparation obtained in example 10 on the number of colonies of peripheral blood and alveolar lavage fluid in rats in pneumonia model

FIG. 2 is a graph showing the effect of the preparation obtained in example 10 on the WBC count and classification in whole blood in pneumonia model rats

FIG. 3 is a graph showing the effect of the preparation obtained in example 10 on serum inflammation in pneumonia model rats

FIG. 4 is a graph showing the effect of the preparation obtained in example 10 on serum immunoglobulin of pneumonia model rats

FIG. 5 is a graph showing the effect of the preparation obtained in example 10 on serum stress factors in pneumonia model rats

FIG. 6 is a graph showing the effect of the preparation obtained in example 10 on lung tissue changes in rats in a pneumonia model

FIG. 7 is a graph showing the effect of the preparation obtained in example 10 on the expression of TLR4 in lung tissue of rats in a pneumonia model

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration and are not intended to limit the scope of the invention.

Example 1: mixing the following traditional Chinese medicine materials according to the weight ratio;

extracting the medicinal materials by steam distillation for 2 times, adding water 20 times the weight of the medicinal materials for the first time, extracting for 4 hr, adding water 8 times the weight of the medicinal materials for the second time, extracting for 1 hr, collecting volatile oil, mixing the extractive solutions, standing, filtering, concentrating under reduced pressure, drying, and mixing with volatile oil to obtain the Chinese medicinal extract.

Example 2: mixing the following traditional Chinese medicine materials according to the weight ratio;

taking the medicinal materials, adding 14 times of water, extracting by steam distillation for 2 times, 4 hours for the first time and 1 hour for the second time, collecting volatile oil, mixing the extractive solutions, standing, filtering, concentrating under reduced pressure, drying, and mixing with volatile oil to obtain the Chinese medicinal extract.

Example 3：

Mixing the following traditional Chinese medicine materials according to the weight ratio;

extracting the medicinal materials for 2 times by adopting a steam distillation method, adding water with the weight 20 times of that of the medicinal materials for the first time, extracting for 4 hours, adding water with the weight 8 times of that of the medicinal materials for the second time, extracting for 1 hour, collecting volatile oil, combining extracting solutions, concentrating the extracting solution to 1.15-1.20, adding 70% ethanol, precipitating the mixture to 60% by alcohol, standing for 24 hours, taking supernate, concentrating, drying, and mixing with the volatile oil to obtain the traditional Chinese medicine extract.

Example 4：

taking the medicinal materials, adding water with the weight 14 times of the medicinal materials, extracting for 2 times by adopting a steam distillation method, carrying out extraction for 4 hours for the first time and 1 hour for the second time, collecting volatile oil, combining extracting solutions, concentrating the extracting solution to 1.15-1.20, adding 95% ethanol, precipitating to 60%, standing for 12 hours, taking supernate, concentrating, drying, and mixing with the volatile oil to obtain the traditional Chinese medicine extract.

Example 5：

Weighing the following traditional Chinese medicine materials according to the weight ratio;

extracting the medicinal materials with ethanol under reflux for 2 times, adding 95% ethanol 12 times, heating and refluxing for 3 hr, adding 30% ethanol 6 times, heating and refluxing for 1 hr, collecting volatile oil, mixing extractive solutions, concentrating under reduced pressure, drying, and mixing with volatile oil to obtain the final product.

Example 6：

Mixing the following traditional Chinese medicine materials according to the weight ratio, and crushing into coarse powder;

pulverizing the raw materials into coarse powder, percolating, adding 70% ethanol 15 times at 3ml/min until no percolate flows out; collecting percolate, concentrating under reduced pressure, and drying to obtain Chinese medicinal extract.

Example 7：

extracting a, b, c, d, g and h for 1 time by adopting a steam distillation method, wherein the dosage of a solvent is 12 times of the weight of the medicinal materials, extracting until the volatile oil is not distilled off, collecting the volatile oil for later use, filtering an extracting solution, concentrating under reduced pressure until the relative density is 1.10-1.15, adding ethanol to the extracting solution until the alcohol content of the medicinal solution is 70%, carrying out ethanol precipitation for 12h, and filtering to obtain a filtrate 1. And extracting e and f with 60% ethanol under reflux for 2 times, wherein the solvent dosage is 10 times and 8 times of the weight of the medicinal materials respectively, each extraction is carried out for 1 hour, filtering is carried out, supernate and the filtrate 1 are combined, and the pressure reduction concentration is carried out until the relative density is 1.15-1.20.

Adding PEG400 with an amount of 1% of the weight of the medicinal materials into the volatile oil, dissolving, mixing, adding into the concentrated solution under stirring, adding appropriate amount of water, sweetener (such as white sugar), essence, and antiseptic, filtering, and making into oral liquid or syrup.

Example 8：

Pulverizing the Chinese medicinal extract of examples 1-3, adding appropriate amount of adjuvants such as filler, binder, disintegrant, lubricant, flavoring agent, etc., mixing, and making into granule or capsule.

Example 9：

cold soaking with 75% ethanol for 2 times, wherein the solvent amount is 15 times of the weight of the medicinal materials for the first time, and the cold soaking extraction time is 48 h; the solvent dosage is 8 times of the weight of the medicinal materials for the second time, and the cold soaking extraction time is 12 h. Filtering, mixing filtrates, adding polyoxyethylene 40 hydrogenated castor oil in an amount of 4% of the medicinal materials, dissolving, and mixing; adding water to adjust the alcohol content of the medicinal liquid to 25% and the medicinal material content to 5%, and making into spray.

Example 10

Percolating with 75% ethanol with solvent 15 times of the medicinal materials at 3ml/min until no percolate flows out, filtering percolate, adding 4% polyoxyethylene 40 hydrogenated castor oil into the filtrate, dissolving and mixing; adding water to adjust the alcohol content of the medicinal liquid to 25% and the medicinal material content to 5%, and making into spray.

Example 11

The preparation obtained in example 10 is used for bacteriostasis test by referring to bacteriostasis liquid standard, and the bacteriostasis effect and safety data are as follows:

the experiment of the general standard technical service (Tianjin) limited company proves that: the composition has the bacteriostatic action on gram-positive bacteria, gram-negative bacteria and fungi.

(1) Inhibitory effect on Staphylococcus aureus

The product has antibacterial effect on Staphylococcus aureus for 2min, and has antibacterial rate of over 99%.

(2) Inhibiting Escherichia coli growth

The product has effect on Escherichia coli for 2min, and has antibacterial rate of over 99%.

(3) Inhibiting effect on candida albicans

The product has effect on Candida albicans for 10min, and has antibacterial rate of above 82%.

Stability experiments prove that the bacteriostatic action of the antibacterial agent can be maintained for more than 1 year at room temperature.

Example 12

The preparation obtained in the embodiment 10 is used for carrying out drug effect and action mechanism research experiments, and according to the characteristics of the formula, a rat model of Klebsiella pneumoniae induced pneumonia is adopted to research the action mechanism of the Klebsiella pneumoniae induced pneumonia under the pathological state of pneumonia. The study data are as follows:

1. in vitro respiratory virus inhibition study

1.1 Experimental materials

Virus strain: human coronavirus (HCoV-229E), provided by the institute of pharmaceutical and biotechnology, national academy of medical sciences; influenza a H1N1 virus (FM1 strain), influenza a H1N1 virus (PR8 strain), parainfluenza-1 (sendai) virus (PIV-1), Respiratory Syncytial Virus (RSV), purchased from the chinese centers for disease control virus.

Test samples to be tested: dilution of the formulation obtained in example 10.

1.2 Experimental methods

The virus solution is respectively mixed with the diluent of the preparation obtained in the example 10 according to the ratio of 1:9, and the mixture is acted for 30 minutes at room temperature; human embryonic lung fibroblast MRC-5 cells were used for HCoV-229E virus infection using DMEM medium; human lung carcinoma cell A549 was used for PR8, FM1, PIV-1 and RSV virus infection using DMEM medium.

Observing cytopathic condition under a daily inverted microscope

Cytopathic effect is judged according to 6-grade standard:

-: the cells grow normally and no disease is generated;

+ -: cytopathic effects are less than 10% of the entire monolayer;

+: cytopathic effects account for less than about 25% of the entire monolayer of cells;

++: cytopathic effects comprise less than about 50% of the total monolayer of cells;

+++: cytopathic effects account for less than about 75% of the total monolayer of cells;

++++: cytopathic effects account for more than about 75% of the total monolayer of cells.

50% Inhibitory Concentration (IC) calculated as Reed-Muench₅₀)。

1.3 results of the experiment

TABLE 1 inhibition of HCoV-229E, PR8, FM1, PIV-1 and RSV viruses by drugs on in vitro cultured cells

1.4 conclusion of the experiment

The composition has an inhibiting effect on MRC-5 cytopathy caused by human coronavirus HCoV-229E infection, and can achieve 100% inhibition at the concentration of 31 mul of liquid medicine/ml; has obvious inhibition effect on A549 cell lesion caused by infection of influenza virus PR8 strain, FM1 strain, parainfluenza virus and respiratory syncytial virus, and can achieve 100 percent inhibition at the concentration of 62 mul of liquid medicine/ml.

2. Effect study on rat pneumonia model

The experiment adopts a bacterial pneumonia model simulated by intrapulmonary injection of Klebsiella pneumoniae to investigate the drug effect and possible mechanism of the composition on pneumonia rats.

2.1 materials of the experiment

Positive control drug: cefalexin tablets.

Reagent: klebsiella pneumoniae (ATCC 9997); rat interleukin-1, 6, 10 kit (Elisa method); rat cyclooxygenase-1 kit (Elisa method); rat tumor necrosis factor-alpha kit (Elisa method); rat bradykinin kit (Elisa method); rat monocyte chemotactic factor-1 (Elisa method); rat immunoglobulin G, M, A (Elisa method); rat dopamine (Elisa method); rat norepinephrine (Elisa method): rat serotonin (Elisa method).

Test samples to be tested: dilution of the formulation obtained in example 10.

The test animals were: rat, strain SD, Beijing Sibefu Biotechnology Ltd, license number SCXK (Beijing) 2019-.

2.2 Experimental methods

After co-culturing the preparation obtained in example 10 with Klebsiella pneumoniae in different volumes for 24h, the bacteria were collected in 2mL of physiological saline per group; 0.2mL of each of the solutions was administered to the lungs for 3 consecutive days. The rats of the administration group were sprayed with different volumes of the formulation obtained in example 10 for 6 days in the environment.

2.3 conclusion of the experiment

The composition can obviously reduce the number of bacterial colonies in peripheral blood and alveolar flushing fluid of a pneumonia model rat;

secondly, the composition can obviously reduce the percentage of WBC, neutrophils and monocytes in the peripheral blood of a pneumonia model rat and increase the percentage of lymphocytes;

the composition can obviously reduce the level of each inflammatory factor in serum of a pneumonia model rat, such as IL-1, IL-6, IL-10, TNF-alpha, COX-1, BK and MCP-1, and can reduce the possibility of inflammatory storm induced when pathogenic bacteria invade a human body;

the composition can obviously reduce the levels of various immune factors such as IgA, IgM and IgG in serum of a pneumonia model rat, and prompts that the composition can inhibit hyperfimmunity induced by pathogenic bacteria invading a human body in vivo and reduce inflammatory reaction;

the composition can obviously reduce the content of serum NE and 5-HT of a pneumonia model rat, relieve vasoconstriction and heart rate acceleration caused by inflammatory stress reaction in the acute stage of pneumonia, and resist physical stress reaction; the product has the tendency of increasing DA content, and can transmit pleasure information and relieve psychological stress reaction caused by pneumonia.

The composition can improve the occurrence of lung tissue inflammatory infiltration caused by Klebsiella pneumoniae, and can reduce the expression of TLR4 protein of lung tissue, thereby inhibiting the release of downstream inflammatory factors and inhibiting hyperfunction of immunity and abnormality of lung ventilation function in an acute state.

In summary, experiments show that the composition has strong in-vitro antibacterial activity, can obviously improve the rat pneumonia phenotype induced by Klebsiella pneumoniae and reduce the cytokine storm induced by hyperfimmunity, the mechanism of the composition is probably realized by inhibiting the activation of a TLR 4/NF-kB signal channel, and the composition can also adjust the physical and psychological reactions under the stress state.

Having now described embodiments of the present invention in detail, it is believed that many modifications and variations will be apparent to those skilled in the art in light of the foregoing disclosure, and it is intended to utilize the invention to its fullest extent without departing from the basic spirit of the invention. All such variations and modifications are to be considered within the scope of the present invention, and the foregoing preferred embodiments are therefore to be considered in all respects illustrative rather than limiting.

Claims

1. A traditional Chinese medicine composition is prepared by extracting the following traditional Chinese medicines in parts by weight:

2. the traditional Chinese medicine composition of claim 1, which is prepared by extracting the following traditional Chinese medicines in parts by weight:

3. the traditional Chinese medicine composition of claim 1, which is prepared by extracting the following traditional Chinese medicines in parts by weight:

4. the Chinese medicinal composition of any one of claims 1 to 3, which can be prepared into any pharmaceutically acceptable dosage form.

5. The Chinese medicinal composition of claim 4, wherein the dosage form is selected from oral solid preparation or liquid preparation.

6. The Chinese medicinal composition of claim 5, wherein the liquid preparation is selected from a spray or an oral liquid preparation.

7. An oral liquid preparation, which comprises the traditional Chinese medicine composition as claimed in any one of claims 1 to 3, one or more of polar solvent, semi-polar solvent or non-polar solvent, and one or more of flavoring agent, coloring agent, preservative and medicinal surfactant.

8. A spray, which comprises the traditional Chinese medicine composition as claimed in any one of claims 1 to 3, medicinal ethanol and a surfactant, wherein the addition amount of the surfactant is 1-10% of the weight of the medicinal materials, preferably 2-6%; the alcohol content is 10-75%, preferably 10-40%, more preferably 20-40%; the content of the medicinal materials is 2 to 20 percent, preferably 2 to 10 percent, and more preferably 3 to 6 percent.

9. The spray according to claim 8, wherein the surfactant is any one or more of an anionic surfactant, a cationic surfactant or a zwitterionic surfactant; the surfactant is preferably polyoxyethylene 40 hydrogenated castor oil.

10. A method of preparing a Chinese medicinal composition as claimed in any one of claims 1 to 3, the method comprising the steps of:

or taking the medicinal materials, adding 6-20 times of 30-95% ethanol, percolating or cold-soaking for 1-4 times, combining the extracting solutions, concentrating under reduced pressure, and drying to obtain a traditional Chinese medicine composition; wherein the percolation speed is 1-5 ml/min until no percolate flows out; the cold soaking extraction time is 6-24 hours each time.

11. A method of preparing a Chinese medicinal composition as claimed in any one of claims 1 to 3, the method comprising the steps of:

12. A process for the preparation of a spray according to claim 8 or 9, which comprises the steps of:

13. The method of claim 12, wherein: the method comprises the following steps:

the percolation speed is 1-5 ml/min until no percolate flows out;

the cold leaching extraction time is 6-72 h.

14. The method of claim 12, wherein: the method comprises the following steps:

mixing the traditional Chinese medicines according to the weight ratio, percolating or cold-soaking the traditional Chinese medicines for 1-3 times by using 50-80% ethanol, filtering, combining the filtrates, adding a surfactant with the weight of 2-6% of the weight of the traditional Chinese medicines, dissolving and uniformly mixing the filtrates, wherein the dosage of the solvent is 8-15 times of the weight of the traditional Chinese medicines; adding water to adjust the alcohol content of the liquid medicine to be 20-40 percent and the medicine content to be 3-6 percent;

the percolation speed is 2-4 ml/min until no percolate flows out;

15. Use of the Chinese medicinal composition of any one of claims 1 to 3 in the preparation of a medicament for inhibiting pathogenic microorganisms on body surfaces, articles and in the air or treating bacterial pneumonia.

16. The use according to claim 15, wherein the pathogenic microorganism is selected from the group consisting of human coronavirus HCoV-229E, influenza PR8 strain and FM1 strain, parainfluenza virus, respiratory syncytial virus infection.