CN112972583A

CN112972583A - Natural herbal eye drops and preparation method and application thereof

Info

Publication number: CN112972583A
Application number: CN202110265484.2A
Authority: CN
Inventors: 王睿超; 王梓翊; 姚晓瑜
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-03-11
Filing date: 2021-03-11
Publication date: 2021-06-18

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to natural herbal eye drops and a preparation method and application thereof. The natural herbal eye drops are prepared from the following active ingredients and auxiliary materials in parts by weight: 5-15 parts of chrysanthemum, 4-12 parts of astragalus membranaceus, 5-15 parts of semen cassiae, 4-12 parts of medlar, 4-12 parts of mulberry, 4-12 parts of red date, 4-12 parts of Chinese yam, 4-12 parts of lotus seed, 4-12 parts of ligusticum chuanxiong hort, 4-10 parts of prepared rehmannia root, 4-12 parts of radix asparagi, 4-12 parts of radix paeoniae alba, 4-12 parts of radix achyranthis bidentatae, 3-10 parts of angelica sinensis and 4-15 parts of tea. The natural herbal eye lotion is scientific and reasonable in components, free of stimulation, remarkable in curative effect and free of side effect, can relieve and treat symptoms such as eye fatigue, eye dryness and eye dryness, photophobia and lacrimation caused by overuse of eyes, does not contain hormone and antibiotics, does not have dependence, and ensures eye health of patients.

Description

Natural herbal eye drops and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to natural herbal eye drops and a preparation method and application thereof.

Background

With the ubiquitous and gradual normalization and activation of various electronic screens, people can not use the electronic screens selectively, and the eye use time is gradually increased, so that the visual fatigue is increased, the eye health is influenced, and the eye dryness, the light intolerance and the lacrimation, the eye diseases and the like follow. In the current overuse age of eyes, visual health has become a social problem that is receiving much attention and urgently awaiting solution.

To alleviate the above-mentioned symptoms of ocular discomfort, current solutions include increasing the resting time of the eyes, supplementing vitamins, and using eye drops. The occasional overuse of eyes can be relieved by increasing the resting time of eyes and supplementing vitamins, but the mode is more aimed at symptoms of short-time asthenopia, and the problems of asthenopia, dry and unsmooth eyes, photophobia and lacrimation and the like which are inevitably caused by long-term work and irregular work and rest of people in life are also assisted by eye drops. Because people have limited understanding on the symptoms of dry eyes and unsmooth eyes, the dry eyes and the unsmooth eyes are simply thought to be only due to the lack of water in the eyes, so that eye drops are purchased by themselves or ordinary eye drops on the market are dropped into the eyes, the symptoms can be only temporarily relieved by the method, the treatment effect cannot be achieved, and accordingly the eye conditions are delayed, and serious consequences such as keratitis, vision deterioration, visual injury and the like can be caused.

The existing eye drops are various in types and generally divided into two types of anti-inflammation and asthenopia relieving. The eye drops for relieving eyestrain mainly comprise sodium hyaluronate, vitamins and the like, can relieve discomfort caused by eyestrain as described above, cannot essentially solve other symptoms such as eyestrain and dry eyes and eye dryness caused by eyestrain, and has certain dependence. The anti-inflammatory eye drops generally contain components such as antibiotics, hormones, preservatives and the like, and the antibiotic eye drops are only suitable for eye infection diseases and cannot be used randomly; the hormone eye drops can cause hormone glaucoma after long-term use, and the eye drops added with the preservative are not suitable for long-term use because the eye drops can stimulate or damage the corneal surface of a normal human eye.

Disclosure of Invention

The natural herbal eye drops have scientific and reasonable components, no stimulation, obvious curative effect and no side effect, can relieve and treat symptoms such as eye fatigue, dry eyes, poor light feeling, lacrimation and the like caused by overuse of eyes, does not contain hormone and antibiotic, has no dependence, ensures the eye health of patients and solves the problems in the prior art.

One of the technical schemes adopted by the invention is as follows:

a natural herbal eye lotion is prepared from the following active ingredients and auxiliary materials in parts by weight:

5-15 parts of chrysanthemum, 4-12 parts of astragalus membranaceus, 5-15 parts of semen cassiae, 4-12 parts of medlar, 4-12 parts of mulberry, 4-12 parts of red date, 4-12 parts of Chinese yam, 4-12 parts of lotus seed, 4-12 parts of ligusticum chuanxiong hort, 4-10 parts of prepared rehmannia root, 4-12 parts of radix asparagi, 4-12 parts of radix paeoniae alba, 4-12 parts of radix achyranthis bidentatae, 3-10 parts of angelica sinensis and 4-15 parts of tea.

Further, the auxiliary materials are hyaluronic acid and vitamin B12.

Further, the auxiliary materials comprise the following components in parts by weight: 15-25 parts of hyaluronic acid and 1210-25 parts of vitamin B; preferably: 18 parts of hyaluronic acid and 1210 parts of vitamin B.

Further, the active ingredients are prepared from the following raw materials in parts by weight:

9 parts of chrysanthemum, 8 parts of astragalus membranaceus, 9 parts of semen cassiae, 6 parts of medlar, 8 parts of mulberry, 8 parts of red date, 8 parts of Chinese yam, 6 parts of lotus seed, 6 parts of ligusticum chuanxiong hort, 6 parts of prepared rehmannia root, 6 parts of radix asparagi, 8 parts of radix paeoniae alba, 6 parts of radix achyranthis bidentatae, 5 parts of angelica and 9 parts of tea.

The second technical scheme adopted by the invention is as follows:

a preparation method of the natural herbal eye drops comprises the following operation steps:

1) mixing and crushing the active ingredients in parts by weight into micro powder;

2) extracting the micro powder obtained in the step 1) with alcohol to obtain a leaching solution;

3) mixing and uniformly stirring the components of the auxiliary materials in parts by weight for later use;

4) concentrating the leaching solution obtained in the step 2) to obtain a concentrated solution, adding a proper amount of purified water and the auxiliary materials obtained in the step 3), and uniformly mixing until the mixture is in a soft liquid state.

Further, the micro powder in the step 1) is mechanically crushed into 20-30 meshes; step 2), ethanol extraction is carried out, wherein 75% ethanol is gradually added into the micro powder, and the addition amount is 5-10 times of the weight of the micro powder; step 3), mixing uniformly by adopting a vortex mixer; and 4) adding water to ensure that the content of active ingredients in the concentrated solution reaches 2-3%.

Further, the water adding amount in the step 4) is that the content of the active ingredients of the concentrated solution in the mixed solution containing the active ingredients of the concentrated solution, the auxiliary materials and the purified water reaches 2-3%.

Further, a vortex mixer of 18000-.

Further, the step 2) of alcohol extraction is carried out by placing micro powder in a percolation cylinder.

Further, after the leaching solution in the step 4) is concentrated until no alcohol smell exists, purified water and auxiliary materials are added.

The third technical scheme adopted by the invention is as follows:

use of a natural herbal eye lotion as described above for the preparation of a medicament for the treatment of ophthalmic disorders.

Further, the ophthalmic diseases include eye fatigue, dry eyes, photophobia and lacrimation.

The usage and dosage of the natural herbal eye drops are as follows:

the use method I comprises the following steps: making into spray, spraying on eyes for 1-2 times.

The use method II comprises the following steps: pouring the eye lotion into a cup for washing by 5mL, immersing the eyes for at least 10 seconds, and slowly raising the head.

The medicinal properties of the active ingredients of the invention are:

chrysanthemum: disperse wind-heat, calm liver yang, clear liver heat and improve vision, clear heat and remove toxicity. Has antioxidant effect, and can prevent cell degeneration and aging, relieve eye fatigue, improve eye function, and improve vision.

Astragalus root: sweet and warm, the essential herb for tonifying middle-jiao and Qi.

Cassia seed: clearing heat and improving eyesight, and can be used for treating conjunctival congestion, swelling and pain, photophobia, lacrimation, and dim and unclear vision.

Medlar: nourishing liver and kidney, benefiting essence and improving eyesight. Internal heat, diabetes, blurred vision and blurred vision. Treating blurred vision. Enhancing nonspecific immunity.

And (3) mulberry fruit: liver meridian and kidney meridian. Yin deficiency of the liver and kidney, dim eyesight, tinnitus, fluid deficiency and blood deficiency.

Red dates: contains vitamin A, and can reduce visual deterioration and protect vision.

Chinese yam: sweet and mild in nature and taste, thick in quality and strong in nourishing property, and can tonify qi and nourish yin; contains multiple vitamins.

Lotus seed: clearing heart fire, removing heat, stopping bleeding, invigorating kidney, relieving eye fatigue, and relieving eye muscle tension.

Ligusticum wallichii: promoting blood circulation and activating qi-flowing, and is rich in various amino acids, vitamin B1, vitamin B2, vitamin E, and microelements such as zinc and copper.

Prepared rhizome of rehmannia: tonify liver and kidney, enrich blood, moisten, activate blood.

Asparagus: to nourish yin, moisten dryness, clear lung-heat and reduce fire. Has therapeutic effects on lung yin deficiency, dry eye, asthenopia, and blurred vision.

White peony root: soothing liver and regulating qi, nourishing liver and blood, relieving pain, calming liver and astringing yin, and tonifying blood.

Achyranthes root: dispel stasis and dredge meridian, draw blood downward, conduct heat and drain downward.

Chinese angelica: tonify blood, activate blood and alleviate pain.

Tea leaf: contains vitamin C, and can effectively reduce eye turbidity, clear liver and improve eyesight, and improve discomfort caused by eye fatigue.

The invention has the beneficial effects that:

the natural herbal eye drops are prepared by mixing active ingredients with synergistic effect, such as chrysanthemum, astragalus membranaceus, semen cassiae, medlar, mulberry, red dates, Chinese yam, lotus seeds, ligusticum chuanxiong hort, radix rehmanniae preparata, radix asparagi, radix paeoniae alba, radix achyranthis bidentatae, angelica sinensis, tea leaves and the like, and auxiliary materials including hyaluronic acid and vitamin B12, and have the advantages of scientific and reasonable components, no stimulation, remarkable curative effect and no side effect; compared with the existing eye drops which mainly take hyaluronic acid, taurine and vitamins as main components, the eye drops can supplement water to eyes, improve the meibomian gland function of patients, greatly reduce the evaporation of tears, ensure the normal secretion of oil and fat layers in the tears and avoid the discomfort and dryness of eyes; eliminates the harm to eye health caused by the eye health, and avoids inflammation and aggravation of disease.

The natural herbal eye drops disclosed by the invention have the advantages that all active ingredients are pure natural ingredients, the eye drops are healthy and have no side effect, have a protective effect on optic nerves, and can eliminate symptoms such as asthenopia, eye ache, dryness and the like caused by long-term use of eyes. Wherein, the active ingredients of the chrysanthemum, the cassia seeds, the medlar, the mulberry, the lotus seeds and the white paeony root tea can benefit the liver and kidney, clear away heat and toxic material and improve the eyesight; radix astragali, rhizoma Dioscoreae, rhizoma Ligustici Chuanxiong, and radix Paeoniae alba have effects of activating qi-flowing, invigorating qi, invigorating spleen, and benefiting eyes; the vitamins contained in the red dates, the lotus seeds and the tea can reduce hypopsia, protect eyesight and eliminate eye muscle tension; the prepared rehmannia root, the white paeony root, the achyranthes root and the Chinese angelica have the effects of enriching blood and activating blood, dredging channels and collaterals and relieving the nervous tension of eyes caused by the fatigue of the eyes; the lotus seed and the asparagus have the effects of clearing away the lung-heat, clearing away the heart-fire, removing dryness and reducing pathogenic fire, have a treatment effect on dry eyes, uneasiness, fear of strong light and non-durable vision, and avoid the formation of eye inflammation, particularly cornea inflammation. The natural herbal eye lotion prepared by mixing hyaluronic acid and vitamin B12 through the synergistic cooperation of the traditional Chinese medicines can treat symptoms such as eye fatigue, dry eyes, poor photophobia and the like caused by long-term use of eyes, has no dependence and quick response, and ensures the eye health of patients.

Detailed Description

In order to clearly illustrate the technical features of the present invention, the present invention is explained in detail by the following embodiments.

Example 1

The natural herbal eye drops are prepared from the following active ingredients and auxiliary materials in parts by weight:

9Kg of chrysanthemum, 8Kg of astragalus, 9Kg of cassia seed, 6Kg of medlar, 8Kg of mulberry, 8Kg of red date, 8Kg of yam, 6Kg of lotus seed, 6Kg of ligusticum chuanxiong hort, 6Kg of prepared rhizome of rehmannia, 6Kg of asparagus, 8Kg of white peony root, 6Kg of achyranthes bidentata, 5Kg of angelica and 9Kg of tea;

the auxiliary materials are prepared by mixing the following raw materials by weight: 18Kg of hyaluronic acid and 1210 Kg of vitamin B.

The preparation method of the natural herbal eye drops comprises the following steps:

1) mixing the above active ingredients, and mechanically pulverizing into 20-30 mesh micropowder;

2) putting the micro powder obtained in the step 1) into a percolation cylinder, continuously adding 75% ethanol solution with the weight 10 times that of the micro powder for percolation, and collecting leachate;

3) mixing the hyaluronic acid and the vitamin B12 in the weight ratio, and uniformly stirring to obtain auxiliary materials for later use;

4) concentrating the leachate obtained in the step 2) until no alcohol smell exists, adding a proper amount of purified water and the auxiliary materials prepared in the step 3) according to the weight of the concentrated solution, wherein the amount of the purified water is used for ensuring that the mass content of the active ingredient concentrated solution in the mixed solution is between 2 and 3 percent, uniformly mixing the concentrated solution and the auxiliary materials in the mixed solution in a vortex mixer to form soft liquid, and performing irradiation sterilization to obtain the product.

Example 2

6Kg of chrysanthemum, 5Kg of astragalus, 7Kg of cassia seed, 6Kg of medlar, 5Kg of mulberry, 6Kg of red date, 5Kg of yam, 6Kg of lotus seed, 5Kg of ligusticum chuanxiong hort, 6Kg of prepared rhizome of rehmannia, 4Kg of asparagus, 6Kg of white peony root, 4Kg of achyranthes bidentata, 4Kg of angelica and 6Kg of tea;

the auxiliary materials are prepared by mixing the following raw materials by weight: hyaluronic acid 16Kg, vitamin B1210 Kg.

The preparation method of the natural herbal eye drops is the same as the preparation process of the example 1, and details are not given.

Example 3

12Kg of chrysanthemum, 10Kg of astragalus, 13Kg of cassia seed, 10Kg of medlar, 9Kg of mulberry, 10Kg of red date, 9Kg of yam, 10Kg of lotus seed, 8Kg of ligusticum chuanxiong hort, 8Kg of prepared rhizome of rehmannia, 9Kg of asparagus, 10Kg of white peony root, 10Kg of achyranthes root, 7Kg of angelica and 12Kg of tea;

the auxiliary materials are prepared by mixing the following raw materials by weight: hyaluronic acid 20Kg, vitamin B1215 Kg.

Example 4

5Kg of chrysanthemum, 4Kg of astragalus, 6Kg of cassia seed, 7Kg of medlar, 6Kg of mulberry, 4Kg of red date, 6Kg of yam, 4Kg of lotus seed, 4Kg of ligusticum chuanxiong hort, 5Kg of prepared rhizome of rehmannia, 4Kg of asparagus, 4Kg of white peony root, 5Kg of achyranthes bidentata, 4Kg of angelica and 6Kg of tea;

the auxiliary materials are prepared by mixing the following raw materials by weight: 15Kg of hyaluronic acid and 1212 Kg of vitamin B.

Example 5

15Kg of chrysanthemum, 11Kg of astragalus, 15Kg of cassia seed, 12Kg of medlar, 12Kg of mulberry, 11Kg of red date, 10Kg of yam, 10Kg of lotus seed, 10Kg of ligusticum chuanxiong hort, 10Kg of prepared rhizome of rehmannia, 12Kg of asparagus, 11Kg of white peony root, 12Kg of achyranthes root, 10Kg of angelica and 15Kg of tea;

the auxiliary materials are prepared by mixing the following raw materials by weight: hyaluronic acid 22Kg, vitamin B1218 Kg.

Example 6

The active ingredients, the auxiliary materials and the preparation method of the natural herbal eye drops are the same as those of the natural herbal eye drops in the embodiment 1, except that the natural herbal eye drops do not contain red dates, tea leaves and asparagus.

Example 7

The active ingredients, adjuvants and preparation method of the natural herbal eye lotion are the same as those of the natural herbal eye lotion of example 1, except that radix asparagi is not contained.

Example 8

The active ingredients, the auxiliary materials and the preparation method of the natural herbal eye drops are the same as those of the natural herbal eye drops in the embodiment 1, except that the natural herbal eye drops do not contain red dates and tea leaves.

First, animal experiment

The animal model and the normal group of the xerophthalmia are observed by the treatment effect of the medicines of the examples and the control group.

Molding: female C57BL/6 mice, 20, were divided into 10 groups of two: model group, treatment group and control group; injecting scopolamine hydrobromide solution subcutaneously to the mouse to inhibit the tear secretion, placing the mouse in front of a room-temperature blower to accelerate the tear evaporation for 15 days, and establishing a mouse dry eye model.

The treatment mode comprises the following steps: the treatment groups were administered the natural herbal eye drops of examples 1-8, respectively, in the following amounts: making into spray, spraying to eyes, spraying for 1-2 times for one time, and spraying to eyes untimely for 7 days; the model group is given physiological saline, and the using amount is the same as that of the treatment group; the control group was administered with a conventional commercially available eye lotion (sodium hyaluronate eye drops) by instillation, and the dose was the same as that of the treatment group.

The treatment period is 4 weeks, and the mice in the model group, the treatment group and the control group are detected for the amount of lacrimal secretion and the trend of staining of the sodium keratectin.

The treatment results are as follows: the detection result of the lacrimal secretion of the mouse shows that the lacrimal secretion and the conjunctival goblet cell number of the xerophthalmia mouse obtained by molding are obviously reduced compared with the normal group of mice. After drug treatment, the tear secretion amount of the dry eye mice is remarkably increased in the treatment groups of examples 1 to 5 of the invention compared with the control group and the treatment groups of examples 6 to 8, and the treatment groups of the invention are better than those of examples 1 to 3, particularly the treatment group of example 1 has the most remarkable effect.

Staining mouse eyeball tissue sections by using a PAS staining method for conjunctival goblet cells, and counting the change trend of the total number of the goblet cells in each group: the number of indexes of the embodiments 1 to 5 of the present invention is also significantly increased, and the effect is most remarkable particularly in the embodiment 1. The tear secretion amount of the dry eye mice in the treatment groups of examples 6 to 8 is significantly lower than that of the treatment groups of examples 1 to 5, and is lower than that of the control group of the commercially available eye drops, particularly as the tear secretion amount of example 7 is the smallest; the tear secretion amount of the dry eye mice in example 6 is higher than that of the mice in examples 7 and 8, but the number of goblet cells is reduced compared with that of the mice in the normal group, and the treatment group is considered to have limited drug effect, and eye inflammation is formed in the treatment process, eye surface lesion is generated, and then the goblet cells are damaged.

Results of change in corneal fluorescein sodium staining: the corneal fluorescein sodium staining of the mice in the normal group is 0 grade, and the corneal fluorescein sodium staining of the mice in the dry eye group is 4 grade. The corneal fluorescein sodium staining of mice in each treatment group of the embodiments 1-5 of the invention is reduced compared with that of a xerophthalmia group, and the corneal fluorescein sodium staining is classified into 1 grade, 2 grade, 1 grade, 2 grade and 3 grade; the control group was graded at 3, and the treatment groups of examples 6-8 were all graded at 4.

Therefore, the natural herbal eye lotion in examples 1 to 5 has significant improvement and treatment effects on dry eyes and dry eyes caused by experimental dry eye syndrome, wherein the combination of active ingredients has significant effects on simultaneously improving tear secretion and the number of goblet cells of a mouse with dry eye syndrome.

Second, clinical observations

The natural herbal eye lotion of example 1 of the present invention was selected for use with commercially available eye drops, sodium hyaluronate eye drops, for patients with dry eye and asthenopia, and the effects and the experience of use were observed and compared. The patient with eye fatigue aims at volunteer patients who work for a long time on a desk, do irregular work and rest and have eye dryness symptoms for at least one week in the morning; the xerophthalmia patient is a volunteer patient with mild clinical diagnosis of xerophthalmia; the patients in the above disease groups are 10 persons each, half a man and half a woman, and 18-60 years old.

2.1 therapeutic effect standard:

the improvement is remarkable: the symptoms of dry eyes and unsmooth eyes basically disappear, and the eyes do not have ache, foreign body sensation and burning sensation; in at least 1 week, eyes are not dry and uncomfortable after the morning of normal work and regular work and rest every day.

Improvement: the symptoms of dry eyes and dry eyes are obviously reduced; within one week, eyes are occasionally dry and astringent after the normal work and regular work and rest every day and in the morning;

and (4) invalidation: dry eyes, sore eyes, foreign body sensation, burning sensation and photophobia are not improved.

2.2 the group of patients with asthenopia and dry eye treated with the drug of example 1 was performed in accordance with the use amount of the present invention. After 1 week of treatment, 9 persons and 8 persons in two groups respectively have results of remarkably improving symptoms and having no uncomfortable symptoms, the curative effect standard of remarkable improvement is compounded, the other 1 person in the group of asthenopia patients is remarkably improved, and the effective rate is 100 percent; 1 other person in the xerophthalmia patient group obviously improves, 1 person is ineffective, and the effective rate is 90%; for the patients who are obviously improved and improved, the eye moistening liquid is not used at intervals of one week, and the eyes have no uncomfortable symptoms; no other adverse reactions during the treatment period.

2.3 adopting the sodium hyaluronate eye drops of the control group to treat asthenopia group patients and xerophthalmia group patients, wherein the treatment period is at least 10 days, 80% of the asthenopia group patients can effectively relieve uncomfortable symptoms such as dry eye, burning sensation and the like, and only 60% of the xerophthalmia group patients are improved, and the rest is ineffective; in both groups of patients with improvement, the eyes were still slightly dry when they were not used at 3-day intervals.

Therefore, the natural herbal eye drops are used for clinically treating xerophthalmia and asthenopia, and have the advantages of obvious effect, short treatment period and no adverse reaction or dependence compared with the existing eye drops.

The above-described embodiments should not be construed as limiting the scope of the invention, and any alternative modifications or alterations to the embodiments of the present invention will be apparent to those skilled in the art.

The present invention is not described in detail, but is known to those skilled in the art.

Claims

1. The natural herbal eye lotion is characterized by being prepared from the following active ingredients and auxiliary materials in parts by weight:

2. The natural herbal eye lotion of claim 1, wherein said excipients are hyaluronic acid and vitamin B12.

3. The natural herbal eye lotion as claimed in claim 2, wherein the adjuvants comprise, in parts by weight: 15-25 parts of hyaluronic acid and 1210-25 parts of vitamin B; preferably: 18 parts of hyaluronic acid and 1210 parts of vitamin B.

4. The natural herbal eye lotion as claimed in claim 1, wherein the active ingredient is prepared from the following raw materials in parts by weight:

5. A process for preparing a natural herbal eye lotion as claimed in any one of claims 1 to 4, comprising the following steps:

6. The preparation method according to claim 5, wherein the micropowder obtained in step 1) is mechanically pulverized into 20-30 mesh; step 2), ethanol extraction is carried out, wherein 75% ethanol is gradually added into the micro powder, and the addition amount is 5-10 times of the weight of the micro powder; step 3), mixing uniformly by adopting a vortex mixer; and 4) adding water to ensure that the content of active ingredients in the concentrated solution reaches 2-3%.

7. Use of a natural herbal eyedrop as claimed in any one of claims 1 to 4 in the preparation of a medicament for the treatment of ophthalmic disorders.

8. The use of claim 7, wherein the ophthalmic condition comprises eye fatigue, dry eye, aversion to light, and tears.