CN112961771A - Photobioreactor system for air purification - Google Patents
Photobioreactor system for air purification Download PDFInfo
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- CN112961771A CN112961771A CN202110213851.4A CN202110213851A CN112961771A CN 112961771 A CN112961771 A CN 112961771A CN 202110213851 A CN202110213851 A CN 202110213851A CN 112961771 A CN112961771 A CN 112961771A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Abstract
The present invention relates to air purification. The present invention provides a system for reducing the concentration of carbon dioxide in the air of subtropical to temperate climatic regions and a method for reducing the concentration of carbon dioxide using the same.
Description
The application is a divisional application, the application date of the original application is 2015, 12, 16, the application number is 201510946118.8, and the name of the invention is 'photobioreactor system for air purification'.
Cross Reference to Related Applications
According to 35U.S. c § 119(e), the present application is a non-provisional patent application claiming the benefit of U.S. provisional patent application No. 62/124,348, entitled "microalgae photobioreactor design for cleaning indoor and outdoor hong kong air", filed 12/16/2014, the disclosure of which is incorporated herein by reference in its entirety.
Technical Field
The present invention relates to air purification. In particular, the present invention relates to a system for reducing the concentration of carbon dioxide in the air using fresh water or photosynthetic microalgae in the ocean. The invention is particularly suitable for use in subtropical and temperate climatic regions with an average temperature of from 15 ℃ to 30 ℃.
Background
The emission of greenhouse gases from vehicles and industries is one of the major contributors to global warming and causes the earth's temperature to rise. Carbon dioxide (CO)2) Accounting for 68% of greenhouse gases emitted by human activities. Various sequestration of CO2Has been widely studied with the aim of reducing CO2Is discharged into the atmosphere. Usually, CO in air2Capture is by physical or chemical means. For example, washing with an aqueous ammonia solution followed by solvent regeneration at high temperature and separation using high pressure physical solvents. Captured CO2Transported offshore geological storage (gibbons et al, 2008).
The purification of air by biological methods is currently considered a better alternative to traditional chemical and physical methods. Biological methods require mild conditions. High temperature, high pressure and environmentally harmful solvents used in conventional methods are excluded in biological processes. Biological processes are also more sustainable. Biological processes utilize photosynthesis to sequester unwanted CO2Conversion to oxygen, thus eliminating the need for physical storage space to store CO2. Removal of CO by biological means in addition to oxygen2Also can produce valuable biomass. These biomasses can be used in the production of biodiesel, fish feed, fertilizers and nutraceuticals and foods (Chisti, 2007).
However, the local climate may affect the performance of the photobioreactor in removing carbon dioxide. It is therefore desirable to provide a photobioreactor system that is tailored to local climatic and environmental factors to efficiently remove carbon dioxide from air.
Disclosure of Invention
Among the numerous biomass organisms, freshwater microalgae are considered the most suitable photosynthetic organisms for application in air purification photobioreactor systems due to their high photosynthetic efficiency (Perrine et al,2012).
Accordingly, the present invention provides a system for purifying air using microalgae, particularly for purifying air in subtropical to temperate climatic regions.
A first aspect of the invention provides a photobioreactor system for reducing carbon dioxide in air in a subtropical to temperate climate zone, the system comprising:
the reactor tank is used for accommodating a microalgae culture medium with the temperature of 15-30 ℃, wherein the initial concentration of Chlorella (Chlorella species) in the microalgae culture medium is 1000000-1500000 cells/ml;
an air inlet for admitting air into the system;
an air pump, an air pressure controller and a sprayer, which are used for delivering air to the culture medium in the form of small bubbles at the flow rate of 0.1-20L/min;
the temperature controller is used for adjusting the temperature of the culture medium to be at the temperature required by work;
a light source for providing the culture medium with an intensity of 50-500 μmol-2s-1Light of PPFD; and
a gas outlet for allowing a purge gas comprising a reduced carbon dioxide concentration to exit the system.
According to a first embodiment of the invention, the area having subtropical to temperate zones is southeast asia.
According to another embodiment of the invention, the region is hong kong.
According to another embodiment of the present invention, the temperature of the culture medium is controlled to be 15 to 30 ℃.
According to another embodiment of the invention, the pH of the medium is maintained between 7 and 9.
According to another embodiment of the present invention, the light source includes a light emitting diode, a fluorescent lamp, sunlight or the three.
In a second aspect the present invention provides a method of reducing carbon dioxide in the air of a subtropical to temperate climate zone, comprising:
providing a bioreactor system;
delivering air to be treated into the bioreactor system through an air inlet;
adjusting the temperature of the culture medium to a temperature required for operation;
the air to be treated is delivered to the culture medium in the form of small bubbles at a flow rate of 0.1-20L/min.
Unlike any existing bioreactor, the photobioreactor system and the method for purifying air using the same of the present invention are suitable for use in subtropical to temperate climates in order to efficiently remove carbon dioxide.
Drawings
Embodiments of the present invention are described in more detail below with reference to the accompanying drawings, in which,
FIG. 1 is a schematic view of a plate-shaped photobioreactor for indoor air purification according to the present invention.
Fig. 2 is a schematic view of a tubular photobioreactor for indoor air purification according to the present invention.
FIG. 3 is a schematic view of the photobioreactor system including six tubular photo-bioreactors for outdoor air purification according to the present invention.
FIG. 4 shows a first embodiment of the invention at different times for CO2Graph of consumption.
FIG. 5 shows a second embodiment of the invention at different times for CO2Graph of consumption.
FIG. 6 shows a third embodiment of the invention at different times in CO2Graph of consumption.
Detailed Description
For the purpose of illustrating the structure and advantages of the invention, reference should be made to the following detailed description taken in conjunction with the accompanying drawings.
As shown in FIG. 1, the photobioreactor system of the present invention includes a plate-shaped reactor tank 110 for containing a culture medium. The reactor tank of the present invention may be transparent, translucent, or reflective so that light can pass through the reactor tank and reach the microalgae contained therein, thereby achieving photosynthesis. The material of the reactor tank of the present invention may be glass, acrylic plastic, polyacrylic plastic or other transparent materials. The microalgal culture medium comprises Chlorella (Chlorella species) at an initial concentration of 1000000-1500000 cells/ml. Chlorella suitable for use in the present invention includes, but is not limited to, Chlorella (Chlorella sp), Chlorella pyrenoidosa (Chlorella pyrenoidosa), Chlorella vulgaris (Chlorella vulgaris), and the like. The reactor tank may or may not be divided by a plurality of partitions 111, thereby arranging different internal flow patterns. For example, the plurality of partitions 111 are constituted by partitions that alternately protrude from opposite sides of the reactor tank. In one embodiment of the invention, the reactor tank is not provided with any partition. In another embodiment of the invention, the reactor tank comprises two bisected partitions to form a gas lift flow pattern. The photobioreactor system of the present invention further includes one or more air inlets 130 to deliver air to be treated into the microalgae medium. The treated air is delivered to the medium as bubbles by spargers or any other suitable method in the art. In one embodiment of the invention, the air inlet extends from the top to the bottom of the reactor tank and delivers air to the culture medium at a flow rate of 0.1-2.0L/min. In another embodiment of the present invention, the treated air directly forms bubbles at the bottom of the culture medium through the air inlet and enters the culture medium at a flow rate of 0.1-2.0L/min. The temperature of the medium is controlled at 15-30 ℃. A water bath or any other temperature control system used in the art may be used in the system of the present invention to control the temperature of the reactor tank. In one embodiment, the temperature of the medium is maintained at 15-30 ℃. In another embodiment, the temperature of the culture medium and the treated air is the same. The photobioreactor system includes a temperature controller (not shown in fig. 1) that maintains the temperature of the reactor tank within a desired temperature range. The medium used to culture the microalgae may be any kind of medium suitable for the growth of the microalgae. Mixed media may also be used. In one embodiment, the medium is BBM medium (Bold's basal medium). In another embodiment, the medium is Bristol's medium. The pH of the medium is maintained at pH 7-9. When the photobioreactor is continuously operated, the culture medium is maintained at a density of 1000000-1500000cells/ml and a pH stably by removing microalgae biomass and replacing fresh culture medium in the reactor tank. In one embodiment, the operational period of the photobioreactor system is two weeks. The density of the medium was diluted back to the initial concentration of 1000000-1500000cells/ml and fresh medium was added back to the reactor tank. Algal biomass can be selectively harvested from the culture medium.
The photobioreactor system further comprises a gas outlet 140 from which the purified gas having a reduced carbon dioxide concentration leaves the reactor tank. In one embodiment, the air outlet is placed in the upper part of the photobioreactor. The treated gas is bubbled into the culture medium at the bottom of the reactor tank and is purged by the microalgae culture, and then the purge gas with reduced carbon dioxide content rises to the top of the reactor tank and exits through the gas outlet. The chlorella in the culture medium consumes carbon dioxide in the treated air for photosynthesis. The medium converts carbon dioxide to oxygen. Thus, the air treated with the medium is purified by reducing the content of carbon dioxide.
The photobioreactor system of the present invention further includes a light source for providing light for photosynthesis of the culture medium. In one embodiment, the light source takes the form of a lamp chamber 120 having an array of LED lamps located around the exterior of the reactor tank 110. Fluorescent lamps, high-pressure sodium lamps, fluorescent mercury lamps, sun lamps or sunlight may also be used. In one embodiment, the light intensity applied to the medium for effective removal of carbon dioxide is 50-500. mu. mol-2s-1PPFD. In an exemplary embodiment, the LED lamp array or other light source may be positioned at a position perpendicular to the direction of the plurality of partitions 111, thereby increasing the photosynthetic rate by maximizing the light reaching the medium in the reactor tank 110.
Fig. 2 shows another embodiment of the photobioreactor, wherein the reactor tank 210 is tubular. The ratio of the diameter to the height (diameter-to-length ratio) of the reactor tank is 1:5 to 1: 10. as shown, the LED lamp array 220 is vertically arranged along a tubular reactor tank, with the air inlet 230 extending to the bottom of the reactor tank to deliver air into the media and the air outlet placed at the top of the reactor tank. The photobioreactor system further includes sensors for monitoring temperature, gas flow rate, light intensity and pH in the reaction tank, while maintaining the temperature, light intensity, gas flow rate and pH in a desired range using corresponding control methods, thereby efficiently removing carbon dioxide in sub-tropical to temperate climates. In this embodiment, a temperature controller 250 is used to monitor the temperature of the microalgal culture medium and adjust the temperature of the culture medium when the temperature is different from an ideal working temperature.
The present invention also provides a method for reducing carbon dioxide in air in a subtropical to temperate climate zone, the method comprising: the invention provides the photobioreactor system, and further comprises a temperature control system for conveying air to the bioreactor system. The method of the present invention for carbon dioxide reduction to purify air comprises maintaining a microalgae culture medium concentration of 1000000-1500000cells/mL at a temperature of 15-30 ℃, adjusting the temperature of the culture medium to correspond to an ideal working temperature, feeding air to be treated into the culture medium at a flow rate of 0.1-2.0L/min, discharging purified gas having a reduced carbon dioxide concentration out of the photobioreactor system, and selectively collecting any biomass produced by the culture medium during photosynthesis.
Fig. 3 shows another embodiment of the photobioreactor, wherein the reactor tank 310 is tubular and used for outdoor air purification applications. The ratio of the diameter to the height (diameter-to-length ratio) of the reactor tank is 1:5 to 1: 10. the material of the reactor tank in the present invention may be glass, acrylic plastic, polypropylene plastic or any other transparent material known in the art. In one embodiment, a series of six tubular reactor tanks are placed in vertical alignment for air purification. An air inlet 320 is installed at the bottom of each reactor tank for feeding air into the microalgae culture, and several sprinklers or any other dispersing device commonly used in the art are used to evenly disperse the air processed through the reactor tank. The gas outlet 330 is installed at the top of the reactor tank. The photobioreactor system further includes a plurality of sensors for monitoring temperature, humidity, gas flow rate, light intensity, and air inlet and outlet CO in the reactor tank2The concentration and the gas flow rate are maintained in the desired range by corresponding control methods, thereby efficiently removing carbon dioxide in subtropical to temperate climates. In this embodiment, natural sunlight is used as the light source for illumination, the living beingThe temperature of the reactor system is equal to the ambient temperature in a subtropical to temperate climate.
The present invention also provides a method for reducing carbon dioxide in air in a subtropical to temperate climate zone, the method comprising: providing a photobioreactor system of the present invention, further comprising delivering air to the bioreactor system to evenly distribute the air entering the photobioreactor system. The method of the present invention for carbon dioxide reduction to purify air comprises maintaining a microalgae culture concentration of 1000000-1500000cells/mL, feeding air to be treated into the culture at a flow rate of 1-20L/min, discharging purified gas reduced from the inlet carbon dioxide concentration out of the photobioreactor system, and selectively collecting any biomass produced by the culture during photosynthesis.
The photobioreactor system demonstrates efficient carbon dioxide purification in subtropical to temperature climatic regions. Microalgae species, culture medium concentration, temperature, gas flow rate and light intensity are selected for efficient carbon dioxide purification in subtropical to temperate climate areas. These areas have a hot, humid summer and a generally mild winter. In particular, the average monthly temperature in these regions is between 15 ℃ and 30 ℃. The photobioreactor system of the present invention is designed for efficient carbon dioxide purification in southeast asia. The photobioreactor system of the present invention is designed for efficient carbon dioxide purification in hong Kong or similar climatic regions.
Three examples are given below to demonstrate the operation of the present invention for air evolution using microalgae in hong Kong, which can significantly reduce CO in the air2The concentration of (c).
Example 1
The embodiment of the invention shown in FIG. 1 was carried out to purify CO2Contaminated air. The reactor is a plate reactor with a single partition. The capacity of the reactor was 4L. The reactor operates indoors. The initial concentration of Chlorella (Chlorella sp) in the modified BBM was 1200000 microalgae cells/ml matrix. The temperature in the reactor tank was 30 ℃. The continuous illumination intensity of the LED device is 400 mu mol/m2s-1The period of operation is two weeks. CO of gas inlet2The concentration was 490ppm and the flow rate was 1000 ml/min. After 24 hours, CO at the outlet2The concentration was measured as 146ppm, 70% CO consumption2. The reactor can maintain over 70% CO over a two week duty cycle2Consumption amount. The graph in FIG. 4 shows CO over a two-week operating period2The consumption performance of (c).
Example 2
The embodiment of the invention shown in FIG. 2 was implemented to purify CO2Contaminated air. The reactor was a tubular reactor having a capacity of 1000 ml. The ratio of the diameter to the height of the reactor was 1: 10. the reactor operates indoors. The initial concentration of chlorella in the modified BBM was 1200000 microalgae cells/ml matrix. The temperature of the reaction tank was 30 ℃. The continuous illumination intensity of the LED device is 400 mu mol/m2s-1 .CO of gas inlet2The concentration was 450ppm and the flow rate was 1000 ml/min. After 24 hours, CO is present at the outlet2The concentration measured was 90ppm, consuming over 80% of CO2. The reactor can maintain over 80 percent of CO in the working time of 250 hours2Consumption amount. The curves in FIG. 5 show the CO over a two-week working period2The consumption performance of (c).
Example 3
The embodiment of the invention shown in FIG. 3 was implemented to purify CO2Contaminated air. The reactor was a tubular reactor having a capacity of 100L. The ratio of the diameter to the height of the reactor was 1: 5. six reactors were placed in a row. The reactor was placed for outdoor operation. The initial concentration of chlorella in the modified BBM was 1200000 microalgae cells/ml matrix. Sunlight provides natural illumination for the culture medium. CO of gas inlet2The concentration was 400ppm and the flow rate was 10L/min. After 72 hours, CO at the outlet2The concentration measured was 45ppm, consuming over 80% of CO2. The reactor can maintain more than 80% of CO within 18 days of working time2The consumption can maintain 40 percent of CO in 30 days of working time2Consumption amount. The curves in FIG. 6 showCO in monthly working hours2The consumption performance of (c).
The foregoing is illustrative of the present invention and is not intended to be exhaustive or to limit the invention to the precise form disclosed. Various modifications and alterations to this invention will become apparent to those skilled in the art.
The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the following claims and their equivalents.
Appendix
Brinell culture medium
Brinell A
| Chemical name | Measurement of |
| Sodium nitrate (NaNO)3) | 20-25g/L |
| Dipotassium hydrogen phosphate (K)2HPO4) | 6-7.5g/L |
| Potassium dihydrogen phosphate (KH)2PO4) | 14-17.5g/L |
| Sodium chloride (NaCl) | 2-2.5g/L |
Brinell B
| Chemical name | Measurement of |
| Calcium chloride (CaCl)2) | 1.5-2g/L |
Brinell C
| Chemical name | Measurement of |
| Magnesium sulfate heptahydrate (MgSO)4.7H2O) | 6-7.5g/L |
The above brinell a, brinell B and brinell C were diluted 100 times as working solutions.
Trace amount culture solution
Trace 1 (alkaline EDTA)
| Chemical name | Measurement of |
| Ethylenediaminetetraacetic acid (EDTA) | 40-50g/L |
| Potassium hydroxide (KOH) | 25-31g/L |
Trace 2 (trace metals)
| Chemical name | Measurement of |
| Zinc sulfate heptahydrate (ZnSO)4.7H2O) | 7.05-8.82g/L |
| Manganese (II) chloride tetrahydrate (MnCl)2.4H2O) | 1.15-1.44g/L |
| Sodium molybdate (Na)2MoO4) | 0.95-1.19g/L |
| Copper (II) sulfate pentahydrate (CuSO)4.5H2O) | 1.25-1.57g/L |
| Cobalt (II) chloride hexahydrate (CoCl)2.6H2O) | 0.32-0.40g/L |
Trace 3 (acidified acid)
| Chemical name | Measurement of |
| Iron (II) sulfate heptahydrate (FeSO)4.7H2O) | 3.98-4.98g/L |
| Sulfuric acid (H)2SO4) | 0.8-1mL/L |
Trace 4 (boric acid)
The working solution was diluted 1000 times for traces 1, 2, 3 and 4.
Claims (10)
1. A photobioreactor system for reducing carbon dioxide in air in a subtropical to temperate climate zone, comprising:
a reactor tank for containing a culture medium comprising chlorella species; the Chlorella includes Chlorella (Chlorella sp), Chlorella pyrenoidosa (Chlorella pyrenoidosa), Chlorella vulgaris (Chlorella vulgaris), or combinations thereof; the initial microorganism concentration in the culture medium is 1000000-1500000 cells/ml; the culture medium is a Brinell culture medium;
the reactor tank is tubular, and the ratio of the diameter to the height is 1:5 to 1: 10; the plurality of reactor tanks are arranged in a vertical column;
an air inlet for inputting air to the system;
an air pump for controlling a flow rate of the air;
an air pressure controller for controlling the air pressure of the air;
a sparger for delivering the air to the medium in the form of small bubbles;
a temperature controller for detecting the temperature of the culture medium and adjusting the temperature of the culture medium to a desired temperature; the temperature of the culture medium is 15 ℃ to 30 ℃;
multiple CO2Concentration sensor for monitoring CO2The concentration of (c);
a light intensity controller for sensing and controlling the intensity of illumination; the illumination intensity is 50 mu mol-2s-1PPFD to 500 mu mol-2s-1PPFD；
A pH meter for monitoring the pH of the medium; the pH value of the culture medium is 7 to 9;
a relative humidity sensor for monitoring the relative humidity of the air; and
and the gas outlet is used for discharging the purified gas with the reduced carbon dioxide concentration out of the system.
2. The system of claim 1, wherein the CO is present in a gas phase2The concentration sensor comprises at least one CO2An inlet concentration sensor and at least one CO2And (4) an outlet concentration sensor.
3. The system of claim 1, wherein the material of the reactor tank is glass, acrylic plastic or polyacrylic plastic.
4. The system of claim 1, wherein the air has a flow rate of 0.1L/min to 20L/min.
5. The system of claim 1, wherein the reactor tank has a capacity of 1000mL of a tubular reactor; the ratio of the diameter to the height of the reactor tank is 1: 10; the initial concentration of chlorella in the reactor tank was 1200000 microalgae cells/mL matrix; the temperature of the reaction tank is 30 ℃; the illumination intensity is 400 mu mol-2s-1(ii) a The flow rate was 1000 mL/min.
6. The system of claim 1, wherein the reactor tank has a capacity of 100L of tubular reactor; the ratio of the diameter to the height of the reactor tank is 1: 5; six reactor tanks are arranged side by side; the initial concentration of chlorella in the reactor tank was 1200000 microalgae cells/mL matrix; natural illumination; the flow rate was 10L/min.
7. A method of reducing carbon dioxide in the air of a subtropical to temperate climate zone using the system of claim 1, comprising the steps of:
s1, delivering air into the system through an air inlet;
s2, adjusting the temperature of the culture medium to 15-30 ℃;
s3, providing light for the culture medium at a light intensity of 50 mu mol-2S-1 PPFD to 500 mu mol-2S-1 PPFD;
s4, delivering the air into the culture medium in the form of small bubbles at a flow rate of 0.1L/min to 20L/min;
s5, obtaining purified air from the air outlet; and
s6, diluting the concentration of the medium to an initial concentration after a preset period;
wherein the initial microorganism concentration in the culture medium is 1000000-1500000 cells/ml.
8. The method of claim 7, wherein the Chlorella comprises Chlorella (Chlorella sp), Chlorella pyrenoidosa (Chlorella pyrenoidosa), Chlorella vulgaris (Chlorella vulgaris), or a combination thereof.
9. The method of claim 7, wherein the culture medium is a Brookfield medium.
10. The method of claim 7, wherein the predetermined period is two weeks.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462124348P | 2014-12-16 | 2014-12-16 | |
| US62/124,348 | 2014-12-16 | ||
| US14/968,931 | 2015-12-15 | ||
| US14/968,931 US20160166985A1 (en) | 2014-12-16 | 2015-12-15 | Photobioreactor system for air purification by using microalgae |
| CN201510946118.8A CN105483001A (en) | 2014-12-16 | 2015-12-16 | Photobioreactor system used for air purification |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510946118.8A Division CN105483001A (en) | 2014-12-16 | 2015-12-16 | Photobioreactor system used for air purification |
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| Publication Number | Publication Date |
|---|---|
| CN112961771A true CN112961771A (en) | 2021-06-15 |
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| CN202110213851.4A Pending CN112961771A (en) | 2014-12-16 | 2015-12-16 | Photobioreactor system for air purification |
| CN201510946118.8A Pending CN105483001A (en) | 2014-12-16 | 2015-12-16 | Photobioreactor system used for air purification |
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| CN110345577A (en) * | 2019-07-17 | 2019-10-18 | 威发智能科技(江门)有限公司 | A kind of indoor biological oxygenation subtracts carbon method and system |
| CN110647196A (en) * | 2019-10-14 | 2020-01-03 | 全元通通讯股份有限公司 | Active indoor planting dynamic carbon dioxide concentration adjusting system |
| KR102588093B1 (en) * | 2021-10-27 | 2023-10-16 | 주식회사 그린아샤 | Air Purification Device using microalgae |
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