CN112961754A - Production system and production method of soybean meal fermented feed additive - Google Patents

Production system and production method of soybean meal fermented feed additive Download PDF

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Publication number
CN112961754A
CN112961754A CN202110210448.6A CN202110210448A CN112961754A CN 112961754 A CN112961754 A CN 112961754A CN 202110210448 A CN202110210448 A CN 202110210448A CN 112961754 A CN112961754 A CN 112961754A
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fermentation
stirring
soybean meal
feed additive
liquid
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方青春
朱小清
李广杰
周盛昌
吴有林
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Gansu Aonong Feed Technology Co ltd
Fujian Aonong Biological Technology Group Co Ltd
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Gansu Aonong Feed Technology Co ltd
Fujian Aonong Biological Technology Group Co Ltd
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Abstract

The invention discloses a production system and a production method of a soybean meal fermented feed additive, and relates to the field of feed additives. The invention has the beneficial effects that: the fermentation cylinder device adopts the piston pressurizing mechanism and the stirring mechanism which are arranged up and down, and the piston pressurizing mechanism is used for periodically pressurizing the interior of the fermentation cylinder device and is matched with the stirring mechanism in a stirring way, thereby providing good conditions for the growth of microorganisms and the contact of the microorganisms and solid particles of bean pulp, reducing the agglomeration phenomenon of the solid particles and improving the mass and heat transfer and fermentation effects.

Description

Production system and production method of soybean meal fermented feed additive
Technical Field
The invention relates to the field of feed additives, in particular to a soybean meal fermented feed additive production system and a production method thereof.
Background
The solid state fermentation technology is based on a three-phase environment consisting of high solids, low water content liquids and gases of appropriate concentration, in which the microorganisms can normally reproduce and utilize the surrounding solid substrate as nutrients or fillers, improving the properties of the solid substrate, increasing the value of the solid substrate utilization, producing high value products.
The soybean meal is a byproduct of oil extraction of soybeans, and various anti-nutritional factors exist in the soybean meal to influence the absorption of organisms on nutrition. In the microbial fermentation process, the microorganisms can produce hydrolytic enzyme to eliminate anti-nutritional factors in the soybean meal, and can improve the content of polypeptides in the soybean meal and improve the digestion and absorption level of animals; in addition, probiotics generated by microbial fermentation have obvious effects on the aspects of improving the intestinal tracts of animals, reducing the use of antibiotics and the like. Therefore, the preparation of fermented soybean meal by microbial fermentation of soybean meal becomes a hotspot of research in the feed industry in recent years, and solid state fermentation is the main mode for producing fermented soybean meal at present.
The existing soybean meal fermentation equipment has the problems of poor mass and heat transfer effects, fermentation dead angles, easy agglomeration, poor microbial growth, low fermentation efficiency and the like in the fermentation process due to single structure and poor functionality.
Disclosure of Invention
Aiming at the problems of poor mass transfer and heat transfer effects, fermentation dead angles, easy agglomeration, poor microbial growth, low fermentation efficiency and the like of soybean meal fermentation equipment in the prior art in the fermentation process, the invention discloses a soybean meal fermentation feed additive production system which sequentially comprises a basic seed liquid preparation device, a seed liquid fermentation device, a mixed bacterium liquid preparation device and a fermentation roller device from front to back, wherein the fermentation roller device comprises a fermentation roller shell, a stirring mechanism and a piston pressurizing mechanism, and the piston pressurizing mechanism and the stirring mechanism are vertically arranged and installed in the fermentation roller shell.
Preferably, rabbling mechanism includes stirring disk body, stirring dish drive casing, is used for control stirring disk body makes rotary motion's rotating assembly and is used for control lifting unit that lifting motion was made to the stirring disk body, the stirring disk body is installed rotating assembly is last, rotating assembly installs lifting unit is last, stirring dish drive casing is installed fermentation cylinder casing below position, lifting unit installs in the stirring dish drive casing.
Preferably, rotating assembly includes the stirring dish pivot and the agitator motor of vertical setting, agitator motor installs lifting assembly is last, stirring dish pivot fixed mounting be in agitator motor's output, more than one stirring disk body center is installed in the stirring dish pivot is last just stirring disk body place plane with stirring dish pivot axis is perpendicular.
Preferably, fermentation liquor through holes for fermentation liquor to pass through are uniformly distributed on the stirring disc body.
Preferably, more than two stirring disc bodies are uniformly distributed on the rotating shaft of the stirring disc along the length direction of the rotating shaft, and fermentation liquor through holes in two adjacent groups of the stirring disc bodies are distributed on the stirring disc bodies in a staggered manner.
Preferably, a conveying pipe row for conveying gas into the fermentation drum shell is arranged below the lifting disc body which is positioned at the lowest position in the fermentation drum shell.
Preferably, the conveying pipe row comprises a main conveying pipe and a branch conveying pipe, more than two branch conveying pipes are uniformly distributed along the length direction of the main conveying pipe, and gas outlet holes for conveying gas are formed in the branch conveying pipes.
Preferably, the piston pressurizing mechanism comprises a compression cylinder and a pressing plate, the compression cylinder is fixedly installed on the upper surface of the top of the fermentation roller shell, the pressing plate is fixedly installed at the movable end of the compression cylinder, and the edge of the pressing plate is installed on the inner wall of the fermentation roller shell and can move up and down along the inner wall of the fermentation roller shell.
Preferably, a metal partition plate is arranged between the stirring mechanism and the piston pressurizing mechanism, and a circulation port for liquid to pass through is formed in the metal partition plate.
The invention also provides a production method of the soybean meal fermented feed additive, which comprises the following steps:
preparing a basic seed solution, namely inoculating bacillus into a culture medium to prepare a primary fermentation seed solution;
adding an aseptic liquid culture medium into the liquid fermentation tank after the air digestion, pouring the primary fermentation seed liquid, and fermenting to obtain a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water to obtain a mixed bacterial liquid;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 1.5-3 percent of corn flour, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and move up and down, and fermenting for 14-18 hours to obtain the required feed additive.
Has the advantages that:
the technical scheme of the invention has the following beneficial effects:
(1) the fermentation cylinder device adopts a piston pressurizing mechanism and a stirring mechanism which are arranged up and down, and the piston pressurizing mechanism is used for periodically pressurizing the interior of the fermentation cylinder device and is matched with the stirring mechanism for stirring, thereby providing good conditions for the growth of microorganisms and the contact of the microorganisms and solid particles of bean pulp, reducing the agglomeration phenomenon of the solid particles, and improving the mass and heat transfer and fermentation effects;
(2) the stirring mechanism adopts a liftable and rotatable stirring disc body arranged in the fermentation drum shell, the position relation between raw materials in different height layers is changed by lifting the disc body, and the position relation between the raw materials in the same height layer is changed by the rotation motion of the disc body, so that the contact chance of bacillus and bean pulp particles in unit time is improved, the agglomeration phenomenon of solid particles is reduced, and uniform fermentation in different areas is realized;
(3) the arrangement of the piston pressurizing mechanism can also be used for balancing the pressure in the fermentation drum shell, particularly the pressure when the stirring mechanism is in a stirring state, so that the stirring of the stirring mechanism is prevented from being influenced by overlarge pressure, and the adverse influence on bacillus caused by local increase of the gas inlet pressure can be reduced;
(4) the feed additive prepared by the production system has excellent viable count and later-period viable stability; in addition, the feed additive can be applied to pig feed to obviously increase the feed intake of pigs, increase the weight gain speed of the pigs and reduce the feed-weight ratio.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a flow chart of a preferred system for producing fermented soybean meal feed additives of the present invention;
FIG. 2 is a schematic view of a preferred fermenter apparatus according to the present invention;
FIG. 3 is a schematic view of the rotating assembly, the lifting assembly and the agitator disk drive housing of the preferred embodiment of the present invention;
FIG. 4 is a top view of a preferred array of delivery tubes according to the present invention.
In the figure:
1-a basic seed liquid preparation device; 2-a seed liquid fermentation device; 3-a mixed bacteria liquid preparation device; 4-a fermentation drum device; 41-fermentation drum shell; 42-a stirring mechanism; 421-stirring disc body; 422-stirring disk driving shell; 423-a rotating assembly; 424-a lifting assembly; 43-a piston pressurization mechanism; 431-a compression cylinder; 432-a platen; 5-fermentation liquor through holes; 6-a first lifting slide rail; 7-a first slider; 8-conveying pipe rows; 81-main conveying pipe; 82-conveying branch pipes; 9-air outlet holes; 10-a feed inlet; 11-a discharge hole; 12-a compression slide; 13-compressing the slide; 14-metal partition plate; 15-flow through ports; 16-an air outlet; 17-the agitator disk rotating shaft; 18-a stirring motor; 19-a lifting motor; 20-lifting screw rod; 21-lifting plate body.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention will be described in detail and completely with reference to the examples of the present invention, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, provided in the examples, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1 and 2, the soybean meal fermented feed additive production system sequentially comprises a basic seed liquid preparation device 1, a seed liquid fermentation device 2, a mixed bacteria liquid preparation device 3 and a fermentation drum device 4 from front to back, wherein the fermentation drum device 4 comprises a fermentation drum shell 41, a stirring mechanism 42 and a piston pressurizing mechanism 43, and the piston pressurizing mechanism 43 and the stirring mechanism 42 are arranged and installed in the fermentation drum shell 41 from top to bottom. The fermentation cylinder device adopts the piston pressurizing mechanism 43 and the stirring mechanism 42 which are arranged up and down, the interior of the fermentation cylinder device 4 is periodically pressurized through the piston pressurizing mechanism 43 and is stirred and matched with the stirring mechanism 42, good conditions are provided for the growth of microorganisms and the contact of the microorganisms and solid particles of bean pulp, the agglomeration phenomenon of the solid particles is reduced, and the mass transfer, heat transfer and fermentation effects are improved.
As a preferred embodiment, the stirring mechanism 42 includes a stirring disk 421, a stirring disk driving housing 422, a rotating assembly 423 for controlling the stirring disk 421 to make a rotating motion, and an elevating assembly 424 for controlling the stirring disk 421 to make an elevating motion, the stirring disk 421 is mounted on the rotating assembly 423, the rotating assembly 423 is mounted on the elevating assembly 424, the stirring disk driving housing 422 is mounted at a position below the fermenting drum housing 41, and the elevating assembly 424 is mounted in the stirring disk driving housing 422.
As shown in fig. 2 and 3, as a preferred embodiment, the rotating assembly 423 includes a vertically disposed stirring disk rotating shaft 17 and a stirring motor 18, the stirring motor 18 is installed on the lifting assembly 424, the stirring disk rotating shaft 17 is fixedly installed at an output end of the stirring motor 18, the center of one or more stirring disk bodies 421 is installed on the stirring disk rotating shaft 17, and the plane of the stirring disk body 421 is perpendicular to the axis of the stirring disk rotating shaft 17.
As a preferred embodiment, fermentation liquid through holes 5 for the passage of the fermentation solution are uniformly distributed on the stirring disc 421.
As a preferred embodiment, more than two stirring disc bodies 421 are uniformly distributed on the stirring disc rotating shaft 17 along the length direction thereof.
In a preferred embodiment, the fermentation liquid through holes 5 of two adjacent groups of stirring discs 421 are distributed on the stirring discs 421 in a staggered manner.
As a preferred embodiment, the lifting assembly 424 includes a lifting motor 19, a lifting screw 20 installed at an output end of the lifting motor 19, and a lifting tray 21 sleeved on the lifting screw 20, and the stirring motor 18 is fixedly installed on the lifting tray 21; the lifting motor 19 is fixedly installed in the stirring disc driving shell 422.
The stirring mechanism 42 is arranged in the fermentation roller shell 41 by adopting a liftable and rotatable stirring disc body 421, the position relation between raw materials at different height layers is changed by lifting the disc body, the position relation between raw materials at the same height layer is changed by the rotation motion of the disc body, so that the contact chance of bacillus and soybean meal particles in unit time is improved, the agglomeration phenomenon of solid particles is reduced, and the uniform fermentation of different areas is realized.
As a preferable embodiment, more than two first lifting slide rails 6 are arranged in the vertical direction on the inner wall of the stirring disc driving housing 422, and a first slider 7 sleeved on the first lifting slide rail 6 is formed on the outer edge of the lifting disc body 21 at a position corresponding to the first lifting slide rail 6.
In a preferred embodiment, a conveying pipe row 8 for conveying gas into the fermentation drum shell 41 is arranged below the lowest lifting tray 21 in the fermentation drum shell 41.
As shown in fig. 4, as a preferred embodiment, the delivery pipe row 8 includes a delivery main pipe 81 and delivery branch pipes 82, two or more delivery branch pipes 82 are uniformly distributed along the length direction of the delivery main pipe 81, and the delivery branch pipes 82 are formed with gas outlet holes 9 for delivering gas.
In a preferred embodiment, the air outlets 9 are uniformly distributed along the length direction of the conveying branch pipe 82 and are arranged at the position of the upper surface of the conveying branch pipe 82.
As shown in fig. 2, the bottom of the fermentation drum shell 41 is provided with the delivery pipe 8 for delivering oxygen into the fermentation drum shell 41, so that the bacillus in different positions can be fully contacted with the oxygen, and the stirring mechanism 42 and the piston pressurizing mechanism 43 are matched, so that the growth activity of the bacillus can be improved, carbon dioxide generated by fermentation of the bacillus can be taken away when the oxygen is contacted with the bacillus, the fermentation time is further shortened, and the fermentation efficiency is improved.
In a preferred embodiment, a feed inlet 10 is formed on the inner wall of the fermentation cylinder housing 41 above the stirring plate 421, and a discharge outlet 11 is formed on the lower surface of the fermentation cylinder housing 41.
In a preferred embodiment, the piston pressurizing mechanism 43 comprises a compression cylinder 431 and a pressing plate 432, the compression cylinder 431 is fixedly installed on the top upper surface of the fermentation cylinder shell 41, the pressing plate 432 is fixedly installed on the movable end of the compression cylinder 431, and the edge of the pressing plate 432 is installed on the inner wall of the fermentation cylinder shell 41 and can move up and down along the inner wall of the fermentation cylinder shell 41.
The piston pressurizing mechanism 43 can be used for balancing the pressure inside the fermentation roller shell 41, particularly the pressure when the stirring mechanism 42 is in a stirring state, so that the stirring of the stirring mechanism 42 is prevented from being influenced by overlarge pressure, and the adverse influence on bacillus caused by local increase of gas inlet pressure can be reduced.
As a preferred embodiment, a compression slide rail 12 is installed on the inner wall of the fermentation cylinder housing 41, the compression slide block 13 is installed at a position where the edge of the pressing plate 432 corresponds to the compression slide rail 12, and the compression slide block 13 is installed on the compression slide rail 12 and can move up and down along the length direction of the compression slide rail 12.
In a preferred embodiment, a metal partition plate 14 is disposed between the stirring mechanism 42 and the piston pressurizing mechanism 43, and the metal partition plate 14 is formed with a flow opening 15 for passing the liquid.
The metal partition plate 14 mainly prevents the piston pressurizing mechanism 43 from being damaged by the instantaneous periodic pressure applied to the stirring plate during the pressurizing process.
In a preferred embodiment, an air outlet 16 is formed on the inner wall of the fermentation cylinder 41 above the metal partition plate 14, and gas such as carbon dioxide generated by fermentation of bacillus is discharged from the air outlet 16.
The invention also provides a production method of the soybean meal fermented feed additive, which comprises the following steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 20-25 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding an aseptic liquid culture medium into the liquid fermentation tank after the air elimination, and pouring the primary fermentation seed liquid, wherein the volume ratio of the aseptic liquid culture medium to the primary fermentation seed liquid is 100: 1, fermenting for 20-25 h at 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 1.5-3 percent of corn flour into a fermentation roller device 4, adjusting the temperature in the fermentation roller device 4 to be 40-50 ℃, controlling a stirring mechanism 42 in the fermentation roller device 4 to rotate and do lifting motion, wherein the rotating frequency is 40 r/min, the lifting frequency is 30 s/time, controlling a piston pressurizing mechanism 43 to periodically add and reduce pressure in the fermentation roller device 4, the adding and reducing pressure frequency is 3 min/time, alternately controlling the stirring mechanism 42 to stir and the piston pressurizing mechanism 43 to add and reduce pressure, finishing one-time stirring and adding and reducing pressure process for 12min, adding and reducing pressure for 6min and stirring for 6min each time, and obtaining the required feed additive after fermenting for 14-18 h;
and in the stirring process, controlling the conveying pipe row 8 to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device 4, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: (1-2).
As a preferable embodiment, the sterile liquid medium in the second step comprises the following raw materials in percentage by weight: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1% of solvent is pure water, and the pH is natural.
The feed additive prepared by the production system has excellent viable count and later-period viable stability; in addition, the feed additive can be applied to pig feed to obviously increase the feed intake of pigs, increase the weight gain speed of the pigs and reduce the feed-weight ratio.
The beneficial effects of the fermented soybean meal feed additive obtained by the technical scheme of the embodiment are further described by several groups of examples and comparative examples.
The first embodiment is as follows:
the embodiment provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, finishing the stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
example two:
the embodiment provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 25 hours at the temperature of 40-45 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 25 hours at the temperature of 40-45 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-45 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, finishing the stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
example three:
the embodiment provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 20 hours at the temperature of 45-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 20 hours at the temperature of 45-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 45-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, finishing the stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
example four:
the embodiment provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 1.5 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring of the stirring mechanism and the pressurizing and depressurizing of the piston pressurizing mechanism, finishing the one-time stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing 6min each time and stirring 6min, and fermenting for 14h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 2.
comparative example one:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding the soybean meal into a common commercially-sold fermentation drum device, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and corn flour accounting for 2 percent of the mass of the soybean meal, adjusting the temperature in the fermentation drum device to be 40-50 ℃, controlling a stirring mechanism in the fermentation drum device to rotate at the rotation frequency of 40 r/min, and fermenting for 16 hours to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example two:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding the soybean meal into a common commercially-sold fermentation drum device, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and corn flour accounting for 2 percent of the mass of the soybean meal, adjusting the temperature in the fermentation drum device to be 40-50 ℃, controlling a stirring mechanism in the fermentation drum device to rotate at the rotating frequency of 40 revolutions per minute, periodically increasing and reducing pressure in the fermentation drum device, wherein the increasing and reducing pressure frequency is 3 min/time, alternately stirring and increasing and reducing pressure, finishing the stirring and increasing and reducing pressure process for 12min, increasing and reducing pressure for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example three:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, 3.0 is multiplied by 109CFU/g of Saccharomyces cerevisiae and 2.0X 109Adding CFU/g lactobacillus into the aqueous solution, then adding glucose, uniformly mixing and stirring until the glucose is dissolved to obtain the composite microbial inoculum; wherein, the composite microbial agent, the purified water and the glucose are mixed according to the proportion: 0.4: 300: 5;
step two, mixing the soybean meal and the compound microbial inoculum prepared in the step one according to the proportion of 1: adding the mixture into a fermentation roller device according to the proportion of 0.6, adjusting the temperature in the fermentation roller device to 35-37 ℃ and sealing, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30 s/time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3 min/time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, completing the processes of stirring and pressurizing and depressurizing for 12min once, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 48 h;
taking out the soybean meal after fermentation is finished, drying the soybean meal at a low temperature, and crushing the soybean meal to obtain a soybean meal fermented feed additive; the drying temperature is 55-60 ℃.
Comparative example four:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and corn flour accounting for 2 percent of the mass of the soybean meal into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and move up and down, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30 s/time, and fermenting for 16 hours to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example five:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation drum device, closing an air outlet at the position above a metal partition plate, adjusting the temperature in the fermentation drum device to be 40-50 ℃, controlling a stirring mechanism in the fermentation drum device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically add and reduce pressure in the fermentation drum device, wherein the adding and reducing pressure frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to add and reduce pressure, finishing stirring and adding and reducing pressure for 12min for one time, adding and reducing pressure for 6min and stirring for 6min each time, and obtaining the required feed additive after fermenting for 16 hours;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example six:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25, adding the mixture into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30 s/time, controlling a piston pressurizing mechanism to periodically add and reduce pressure in the fermentation roller device, the adding and reducing pressure frequency is 3 min/time, alternately controlling the stirring of the stirring mechanism and the adding and reducing pressure of the piston pressurizing mechanism, finishing the process of stirring and adding and reducing pressure for 12min, adding and reducing pressure for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example seven:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, preparing a basic seed solution, namely inoculating saccharomyces cerevisiae into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a primary fermentation seed solution;
step two, adding the following raw materials in percentage by weight into the liquid fermentation tank after air digestion: glucose: peptone: potassium dihydrogen phosphate: magnesium sulfate: 3% of urea: 2%: 0.3%: 0.15%: 1%, pure water as solvent, aseptic liquid culture medium of natural PH, and primary fermentation seed liquid, wherein the volume ratio of aseptic liquid culture medium to primary fermentation seed liquid is 100: 1, fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the secondary fermentation seed liquid to the hot water is 1: 4;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, finishing the stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example eight:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
preparing a basic seed solution, namely inoculating bacillus into a culture medium, and fermenting for 24 hours at the temperature of 40-50 ℃ to prepare a fermented seed solution;
adding the fermented seed liquid into hot water at the temperature of 70 ℃ to prepare a mixed bacterial liquid, wherein the volume ratio of the fermented seed liquid to the hot water is 1: 4;
step three, mixing the soybean meal and the mixed bacterial liquid prepared in the step two according to the mass ratio of 1: 1.25 adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 2 percent of corn flour into a fermentation roller device, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and lift, wherein the rotating frequency is 40 revolutions per minute, the lifting frequency is 30s per time, controlling a piston pressurizing mechanism to periodically pressurize and depressurize the interior of the fermentation roller device, the pressurizing and depressurizing frequency is 3min per time, alternately controlling the stirring mechanism to stir and the piston pressurizing mechanism to pressurize and depressurize, finishing the stirring and pressurizing and depressurizing process for 12min, pressurizing and depressurizing for 6min and stirring for 6min each time, and fermenting for 16h to obtain the required feed additive;
controlling the conveying pipe row to introduce a mixture of oxygen and nitrogen with the pressure of 3MPa into the fermentation drum device in the stirring process, wherein the mixing volume ratio of the oxygen to the nitrogen is 1: 1.5.
comparative example nine:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, adding 150000U/g acid protease, 200000U/g alkaline protease and 100000U/g neutral protease into an aqueous solution, then adding glucose, uniformly mixing and stirring until dissolving to obtain a compound enzyme solution; wherein, the complex enzyme preparation, the purified water and the glucose are mixed according to the proportion of 0.15: 300: 5.
step two, mixing the compound enzyme solution according to the proportion of 0.6: the soybean meal is inoculated into the soybean meal raw material according to the proportion of 1, uniformly stirred and fermented for 50 hours in a sealing way at the temperature of 35-37 ℃.
Step three, drying and crushing the soybean meal at low temperature after fermentation is finished to obtain a soybean meal fermented feed additive; the drying temperature is 55-60 ℃.
Comparative example ten:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, crushing bean pulp into 2.0 mm;
step two, according to the bean pulp: acid protease: the water content is 1000: 5: 500, the acid protease in this comparative example was produced from Aspergillus niger, the enzyme activity of which was 50000U/g;
step three, controlling the temperature to carry out enzymolysis at 45 ℃, wherein the enzymolysis time is 6 hours;
step four, drying at a low temperature of 135 ℃ by using a dryer;
and step five, crushing the materials to 0.1mm to obtain the soybean meal fermented feed additive.
Comparative example eleven:
the comparative example provides a production method of a soybean meal fermented feed additive, which comprises the following specific steps:
step one, 3.0 is multiplied by 109CFU/g of Saccharomyces cerevisiae and 2.0X 109Adding CFU/g lactobacillus into the aqueous solution, then adding glucose, uniformly mixing and stirring until the glucose is dissolved to obtain the composite microbial inoculum; wherein, the composite microbial agent, the purified water and the glucose are mixed according to the proportion: 0.4: 300: 5;
step two, the composite microbial inoculum is prepared according to the proportion of 0.6: the soybean meal is inoculated into the soybean meal raw material according to the proportion of 1, uniformly stirred and fermented for 48 hours in a sealing way at the temperature of 35-37 ℃.
Step three, drying and crushing the soybean meal at low temperature after fermentation is finished to obtain a soybean meal fermented feed additive; the drying temperature is 55-60 ℃.
Viable count detection is carried out on the soybean meal fermented feed additive and the soybean meal raw materials in four groups of examples and eleven groups of comparative proportions of the invention and recorded in table 2, in the viable count detection, a control group is the soybean meal raw material, a test group 1 is an example I, and the relation between the test group and the examples and the comparative examples can refer to a feeding test; the effects of the soybean meal fermented feed additives of four groups of examples and eleven groups of comparative examples of the invention are tested by feeding tests.
By adopting single factor design, 640 outer three pigs (half of the male and female pigs) with close health conditions and weights are selected, and are randomly divided into 16 treatment groups (each treatment group is 5 repeating groups, and 8 pigs in each repeating group) which are respectively a control group and 15 test groups (test group 1, test group 2, test group 3, test group 4, test group 5, test group 6, test group 7, test group 8, test group 9, test group 10, test group 11, test group 12, test group 13, test group 14 and test group 15). The 16 treatment groups were fed with basal diets, the composition of which is shown in table 1. Wherein, 15 soybean meal fermented feed additives prepared under different process conditions are added in the basic ration adopted by 15 test groups (the addition proportion of the soybean meal fermented feed additives is 1 percent of the basic ration): the experimental group 1 is added with the soybean meal fermented feed additive in the example I, the experimental group 2 is added with the soybean meal fermented feed additive in the example II, the experimental group 3 is added with the soybean meal fermented feed additive in the example III, the experimental group 4 is added with the soybean meal fermented feed additive in the example IV, the experimental group 5 is added with the soybean meal fermented feed additive in the comparative example I, the experimental group 6 is added with the soybean meal fermented feed additive in the comparative example II, and the like.
TABLE 1 basic diet composition
Figure BDA0002952080250000211
Figure BDA0002952080250000221
The premix in table 1 refers to a commercially available common swine premix that provides VA 5 ten thousand IU, VD per kilogram of diet33.75KIU,VE 125IU,VK3 12.5mg,VB1 18.75mg,VB2 44mg,VB6 12.5mg,VB1275 mu g, 250mg of nicotinic acid, 7.5mg of folic acid, 150mg of pantothenic acid, 2.5mg of biotin, 3.12g of choline chloride and 2.0g of compound trace elements.
Feeding test is carried out according to the requirements, wherein the pre-feeding period is 7d, and the testing period is 60 d. During the test period, the feeding management program and the immunization program are carried out according to the conventional commercial pig feeding management manual, the material consumption in the test period is recorded and weighed, the pig is fed for 60 days, and after the test is finished, all indexes of the production performance of the pig are measured. The test data are analyzed by variance, and are subjected to multiple comparisons by a DUNCAN method, P is less than 0.05 is used as a judgment standard of difference significance, and all indexes of the production performance of the pigs are shown in table 2.
TABLE 2 pig Productivity indexes and number of live bacteria in additives
Figure BDA0002952080250000222
Figure BDA0002952080250000231
As can be seen from table 2, the production performance indexes and the additive viable bacteria number of the pigs in test group 1 are obviously superior to those of test groups 5 and 6 and a control group, which indicates that when the fermentation feed additive for soybean meal is produced, compared with the common fermentation roller devices sold on the market, the fermentation roller device provided by the invention can provide good conditions for the growth of microorganisms and the contact between the microorganisms and the soybean meal solid particles in the fermentation process, improve the contact chance between bacillus and the soybean meal particles in unit time, reduce the agglomeration of the solid particles, realize uniform fermentation in different areas, and improve the mass and heat transfer and fermentation effects; in addition, all indexes of the production performance of the pigs and the viable count of the additives in the test groups 1-4 are obviously superior to those of a control group and the test groups 7-15, which shows that the feed additive fermented by the soybean meal can obviously increase the feed intake of the pigs, improve the weight gain speed of the pigs and reduce the feed conversion efficiency, namely reduce the feed-weight ratio when being applied to the pig feed.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The utility model provides a soybean meal fermentation feed additive production system, its characterized in that includes basic seed liquid preparation facilities, seed liquid fermenting installation, mixed fungus liquid preparation facilities and fermentation cylinder device from the front to back in proper order, the fermentation cylinder device includes fermentation cylinder casing, rabbling mechanism and piston loading system, piston loading system with the rabbling mechanism sets up from top to bottom and installs in the fermentation cylinder casing.
2. The soybean meal fermented feed additive production system according to claim 1, wherein the stirring mechanism comprises a stirring disc body, a stirring disc driving shell, a rotating assembly for controlling the stirring disc body to make rotating motion and a lifting assembly for controlling the stirring disc body to make lifting motion, the stirring disc body is installed on the rotating assembly, the rotating assembly is installed on the lifting assembly, the stirring disc driving shell is installed at a position below the fermentation drum shell, and the lifting assembly is installed in the stirring disc driving shell.
3. The soybean meal fermented feed additive production system according to claim 2, wherein the rotating assembly comprises a vertically arranged stirring disc rotating shaft and a stirring motor, the stirring motor is installed on the lifting assembly, the stirring disc rotating shaft is fixedly installed at an output end of the stirring motor, more than one stirring disc body is centrally installed on the stirring disc rotating shaft, and the plane where the stirring disc body is located is perpendicular to the axis of the stirring disc rotating shaft.
4. The system for producing the soybean meal fermented feed additive according to claim 3, wherein fermentation liquid through holes for a fermentation solution to pass through are uniformly distributed on the stirring disc body.
5. The soybean meal fermented feed additive production system according to claim 4, wherein more than two stirring disc bodies are uniformly distributed on the stirring disc rotating shaft along the length direction, and fermentation liquid through holes on two adjacent groups of stirring disc bodies are distributed on the stirring disc bodies in a staggered manner.
6. The soybean meal fermented feed additive production system according to claim 5, wherein a conveying pipe row for conveying gas into the fermentation drum shell is arranged below the lifting disc body which is positioned at the lowest position in the fermentation drum shell.
7. The system for producing the soybean meal fermented feed additive according to claim 6, wherein the conveying pipe row comprises a main conveying pipe and at least two branch conveying pipes, the at least two branch conveying pipes are uniformly distributed along the length direction of the main conveying pipe, and gas outlet holes for conveying gas are formed in the branch conveying pipes.
8. The soybean meal fermented feed additive production system according to claim 1, wherein the piston pressurizing mechanism comprises a compression cylinder and a pressing plate, the compression cylinder is fixedly installed on the upper surface of the top of the fermentation drum shell, the pressing plate is fixedly installed at the movable end of the compression cylinder, and the edge of the pressing plate is installed on the inner wall of the fermentation drum shell and can move up and down along the inner wall of the fermentation drum shell.
9. The system for producing the soybean meal fermented feed additive according to claim 1, wherein a metal partition plate is arranged between the stirring mechanism and the piston pressurizing mechanism, and a circulation port for liquid to pass through is formed on the metal partition plate.
10. A production method of a soybean meal fermented feed additive is characterized by comprising the following steps:
preparing a basic seed solution, namely inoculating bacillus into a culture medium to prepare a primary fermentation seed solution;
adding an aseptic liquid culture medium into the liquid fermentation tank after the air digestion, pouring the primary fermentation seed liquid, and fermenting to obtain a secondary fermentation seed liquid;
adding the secondary fermentation seed liquid into hot water to obtain a mixed bacterial liquid;
step four, mixing the soybean meal and the mixed bacterial liquid prepared in the step three according to the mass ratio of 1: 1.25, adding neutral protease accounting for 0.5 percent of the mass of the soybean meal and 1.5-3 percent of corn flour, adjusting the temperature in the fermentation roller device to be 40-50 ℃, controlling a stirring mechanism in the fermentation roller device to rotate and move up and down, and fermenting for 14-18 hours to obtain the required feed additive.
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Application publication date: 20210615