CN112960780B - Pretreatment method of biomembrane carrier and biological sewage treatment process - Google Patents
Pretreatment method of biomembrane carrier and biological sewage treatment process Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1205—Particular type of activated sludge processes
- C02F3/1215—Combinations of activated sludge treatment with precipitation, flocculation, coagulation and separation of phosphates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/02—Temperature
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a pretreatment method of a biomembrane carrier and a biological sewage treatment process, wherein the pretreatment method of the biomembrane carrier comprises the step of soaking the biomembrane carrier in a pretreatment solution containing activating bacteria, the soaking condition is 10-20 ℃ for 1-3 days, and the activating bacteria are strains secreting NADH oxidase. NADH oxidase secreted by the pretreated biomembrane carrier-coated activated bacteria can promote the microorganisms to produce polysaccharide polymers, promote the glycolysis of the microorganisms and avoid the inhibition of the microorganisms by peroxide. When the method for pretreating the biological membrane carrier is applied to sewage treatment, the biological membrane can be promoted to be quickly started, and the biological membrane can be quickly started even under the condition of low temperature; and the operations such as screening, domestication or transgenosis and the like are not needed to be carried out on the microorganisms, the formed biological membrane has a stable structure, high microorganism density and good species diversity, and has a good application prospect in the field of sewage treatment.
Description
Technical Field
The invention relates to the field of sewage treatment, in particular to a pretreatment method of a biological membrane carrier and a biological sewage treatment process.
Background
The biological sewage treating process includes treating polluted water body via biological action, creating micro environment suitable for bacteria to grow, enriching microbes to form biomembrane, and eliminating organic matter and nitrogen-containing inorganic matter. According to different treatment equipment and operation modes, the biological sewage treatment process comprises a biological membrane sewage treatment process and an activated sludge process treatment process, and the biological membrane sewage treatment process can be further divided into a biological filter tank method, a biological rotating disc method, a biological contact oxidation method, a biological fluidized bed method and the like.
According to the growth mode of microorganisms in the biofilm, the biofilm sewage treatment process can be called as a fixed mode biofiltration technology, microorganism cells usually secrete a plurality of extracellular polysaccharide polymers, so that the microorganisms are attached to and grow on the surface of a fixed carrier (or called as a filler), and the polymerization products such as a microorganism community, polysaccharides and the like and certain types of protozoa become the components of the biofilm and become functional units for degrading sewage.
The biofilm formation time of the biological membrane determines the starting time of the sewage treatment plant. The biofilm formation time of the natural biofilm formation method is generally more than 30 days, and under unfavorable conditions, such as the temperature of sewage water in winter is below 10 ℃, the biofilm formation time of the biofilm can be further prolonged, so that the starting time of a sewage treatment plant is remarkably prolonged, and the activity of microorganisms can be inhibited at low temperature, so that the sewage treatment efficiency of the biofilm is influenced. Patent CN106430528A discloses a method for quickly starting a moving bed biofilm reactor under low temperature, which promotes microorganism attachment and domestication of microorganisms by modifying the surface of a filler to shorten the formation of a biofilm under low temperature, but the efficiency of polysaccharide polymers generated by microorganisms under low temperature is low, so the adhesion of microorganisms to a carrier is not ideal, and meanwhile, the low temperature domestication of microorganisms can reduce the species diversity of communities on the biofilm. Patent CN104973679A discloses a quick start method based on biological filter in low temperature environment, which promotes the start of biological membrane by heating the filter, but it is not suitable for large scale sewage treatment and needs to consume a large amount of energy.
The prior art quick biofilm starting method has complex process and high energy consumption or the variety of the generated biofilms is poor.
Disclosure of Invention
The invention provides a pretreatment method of a biological membrane carrier, which has the advantages of simple treatment process, energy conservation and environmental protection, can quickly start the biological membrane, and can keep the biological membrane to have more complex species diversity.
The pretreatment method of the biomembrane carrier comprises the step of soaking the biomembrane carrier in a pretreatment solution containing activating bacteria, wherein the soaking condition is 10-20 ℃ for 1-3 d, and the activating bacteria are strains secreting NADH oxidase.
Compared with the prior art, the method disclosed by the invention has the advantages that the biological membrane carrier is soaked in the pretreatment solution containing the activator bacteria, so that the biological membrane carrier coated with the activator bacteria is obtained. Putting the pretreated biomembrane carrier into the sewage, enabling the activator to secrete NADH oxidase into a microenvironment on the surface of the biomembrane carrier, and activating a signal path on the biomembrane after the microorganisms in the sewage are contacted with the NADH oxidase on the surface of the biomembrane carrier so as to promote the microorganisms to generate extracellular polysaccharide polymers and promote the microorganisms to generate extracellular polysaccharide polymersThe organisms are adhered to the surface of the biological membrane carrier; NADH oxidase enters the microbial cells and can promote the conversion of NADH in the microbial cells to NAD +, thereby switching the NADH oxidation pathway from the oxidative phosphorylation pathway for forming a large amount of ATP to the NADH oxidase pathway for generating water and ensuring the high NAD + level and the low ATP level required by high-speed glycolysis; microorganisms in wastewater readily absorb or produce peroxides such as H 2 O 2 Etc., which inhibit the proliferation of microorganisms, while NADH oxidase oxidizes and reduces peroxide to water inside cells to protect the microorganisms from the inhibition of peroxide. The NADH oxidase secreted by the activated bacteria coated by the pretreated biomembrane carrier can promote the quick start of the biomembrane, can be quickly started even under the low-temperature condition, does not need to perform operations such as screening, domestication or transgenosis on microorganisms, has stable structure of the formed biomembrane, high microorganism density and good species diversity, has good application prospect in the field of sewage treatment, can quickly generate the biomembrane when being applied to sewage, even sewage with the temperature of 6 ℃, plays a role in purifying the sewage, has simple process, is energy-saving and environment-friendly, and saves time.
Further, the activating bacteria is at least one of bacillus and lactobacillus. The bacillus and the lactobacillus have high efficiency of secreting NADH oxidase, and can grow and proliferate normally in a sewage environment to secrete NADH oxidase continuously.
Furthermore, the addition amount of the activating bacteria is 10-200 mg/L. The addition amount of the activating bacteria is controlled in a certain range, so that the activating bacteria in the pretreatment solution can quickly proliferate and can be effectively adsorbed on the biological membrane carrier.
Further, the biofilm carrier is subjected to surface modification treatment. The adsorption efficiency and the adsorption total amount of the active bacteria and the microorganisms in the sewage can be improved through surface modification treatment. Further, the surface modification treatment is at least one of liquid phase oxidation treatment, ultrasonic treatment, water bath treatment and treatment with a polymer modifier. The polymeric modifier may be an acrylic resin.
Further, the pretreatment solution comprises a wastewater treatment microorganism. The sewage treatment microorganisms refer to nitrifying bacteria and the like which generate degradation to organic matters and ammonia nitrogen compounds in a biological sewage treatment process or microorganisms which generate polysaccharide polymers in a biofilm latent period, and sewage treatment microorganisms can be provided for a pretreatment solution by adding a sewage treatment microorganism solution, powder or activated sludge to the pretreatment solution. Activated sludge refers to sludge in sewage from sewage treatment plants that is enriched in various microorganisms required in sewage treatment. After the sewage treatment microorganisms are added into the pretreatment solution, NADH oxidase secreted by activated bacteria in the pretreatment solution can promote the growth of the sewage treatment microorganisms and adhere to the biofilm carrier, so that the proportion of the microorganisms which are beneficial to biofilm formation and purification in the biofilm carrier is improved, the wall hanging time of the biofilm is further shortened, and the purification efficiency is improved.
Further, the pretreatment solution comprises a bioflocculant. The bioflocculant is a special macromolecular metabolite which is produced by microorganisms and can coagulate and precipitate solid suspended particles which are not easy to degrade in liquid. It is rich in polysaccharide and protein, and can be combined with biological membrane carrier to promote microorganism adhesion.
The invention provides a biological sewage treatment process, which comprises the starting of a biological membrane carrier in sewage, wherein the biological membrane carrier is pretreated by the pretreatment method of the biological membrane carrier.
The biological sewage treatment process has the advantages of quick start of the biological membrane, good species diversity of the biological membrane and high purification efficiency.
Furthermore, the temperature of the sewage is 6-38 ℃. The biological sewage treatment process can quickly generate a biological film in a low-temperature environment.
Further, the biological sewage treatment process may be a biofilm sewage treatment process or an activated sludge sewage treatment process, as long as the start-up step of the biofilm carrier of the present invention is included.
Further, the COD value of the sewage is 100-700 mg/L.
Detailed Description
To better illustrate the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to specific examples. It should be noted that the following examples are provided to further illustrate the present invention and should not be construed as limiting the present invention. The starting materials used in the examples are commercially available, except where otherwise indicated.
The effective specific surface area of the filler A described below was 500m 2 /m 3 The material is high-density polyethylene, meets the standard of A-type filler in the urban construction industry standard CJ/T461-2014 of the people's republic of China, and can also use other conventional biomembrane carriers in the industry. The biological flocculant is dry powder of lactic acid bacteria metabolite, and can also be other biological flocculants. The activated sludge is from a biological sewage treatment tank of Longjiang environmental protection group Limited company, the sampling time is 12 months, the activated sludge is from the same batch of activated sludge samples, and other activated sludge sources can be selected.
Example 1
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and performing liquid-phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the filler A serving as a biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus, 200mg of activated sludge and 5mg of bioflocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus, the nitrobacteria and the filler through aeration, attaching the bacillus, the nitrobacteria and the filler to the surface of a filler adhesion layer to form a microorganism attachment layer, stopping aeration after reacting for 3d at the water temperature of 10 ℃, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Biofilm carrier initiation: and (3) introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of biofilm carrier starting is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be microscopically detected, and the time for completing biofilm formation and the final effluent COD value are recorded.
Example 2
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and (3) performing liquid phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus, 200mg of activated sludge and 5mg of bioflocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus, the nitrobacteria and the filler by aeration, attaching the bacillus, the nitrobacteria and the filler to the surface of the adhesive layer of the filler to form a microorganism attachment layer, stopping aeration after reacting for 1d at the water temperature of 20 ℃, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Starting a biofilm carrier: and (3) introducing sewage with the water temperature of 20 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of biofilm carrier starting is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be microscopically detected, and the time for completing biofilm formation and the final effluent COD value are recorded.
Example 3
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and performing liquid-phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the filler A serving as a biofilm carrier.
(2) Preparation of a pretreatment solution: adding 200mg of lactobacillus, 200mg of activated sludge and 5mg of biological flocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the lactobacillus, the nitrobacteria and the filler through aeration, attaching the lactobacillus, the nitrobacteria and the filler to the surface of a filler adhesion layer to form a microorganism attachment layer, stopping aeration after reacting for 3d at the water temperature of 10 ℃, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Starting a biofilm carrier: introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of the start of a biofilm carrier is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoa and metazoa can be microscopically detected, and the time for completing the biofilm formation and the final COD value of the effluent are recorded.
Example 4
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: filler A was selected as the biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus, 200mg of activated sludge and 5mg of bioflocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus, the nitrobacteria and the filler through aeration, attaching the bacillus, the nitrobacteria and the filler to the surface of a filler adhesion layer to form a microorganism attachment layer, stopping aeration after reacting for 3d at the water temperature of 10 ℃, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Biofilm carrier initiation: and (3) introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of biofilm carrier starting is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be microscopically detected, and the time for completing biofilm formation and the final effluent COD value are recorded.
Example 5
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and (3) performing liquid phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus and 5mg of bioflocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus with the filler through aeration, attaching the bacillus to the surface of the adhesive layer of the filler to form a microorganism attachment layer, reacting for 3d at the water temperature of 10 ℃, stopping aeration, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Starting a biofilm carrier: and (3) introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of biofilm carrier starting is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be microscopically detected, and the time for completing biofilm formation and the final effluent COD value are recorded.
Example 6
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and (3) performing liquid phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus and 200mg of activated sludge into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus, the nitrobacteria and the filler by aeration, attaching the bacillus, the nitrobacteria and the filler to the surface of the adhesive layer of the filler to form a microorganism attachment layer, reacting for 3d at the water temperature of 10 ℃, stopping aeration, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Biofilm carrier initiation: introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of the start of a biofilm carrier is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoa and metazoa can be microscopically detected, and the time for completing the biofilm formation and the final COD value of the effluent are recorded.
Example 7
A biological sewage treating process includes the start-up of pretreated biomembrane carrier in sewage.
The pretreatment method of the biomembrane carrier comprises the following steps:
(1) Selecting a biological membrane carrier: and (3) performing liquid phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the biofilm carrier.
(2) Preparation of a pretreatment solution: adding 10mg of bacillus, 200mg of activated sludge and 5mg of bioflocculant into each 1L of LB culture medium, uniformly mixing to obtain a pretreatment solution, and transferring the pretreatment solution into a moving bed biofilm reactor.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting the bacillus, the nitrobacteria and the filler through aeration, attaching the bacillus, the nitrobacteria and the filler to the surface of a filler adhesion layer to form a microorganism attachment layer, stopping aeration after reacting for 3d at the water temperature of 10 ℃, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Biofilm carrier initiation: and (3) introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 100mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of the start of a biofilm carrier is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be detected by a microscope, and the time for completing the biofilm formation and the final COD value of the effluent are recorded.
Comparative example 1
A biological sewage treatment process comprises the starting of a biological membrane carrier.
(1) Selecting a biological membrane carrier: the filler A is used as a biofilm carrier.
(2) Starting a biofilm carrier: adding the biofilm carrier into a moving bed biofilm reactor, then introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L until biofilm formation is completed, wherein the standard for evaluating the completion of the start of the biofilm carrier is that the COD value of the effluent of the reactor keeps stable, a yellow biofilm is formed on the surface of a filler, protozoon and metazoon can be detected by a mirror, and the time for completing the biofilm formation and the final COD value of the effluent are recorded.
Comparative example 2
A biological sewage treatment process comprises the starting of a biological membrane carrier.
(1) Selecting a biological membrane carrier: and (3) performing liquid phase oxidation, water bath treatment and acrylic resin coating treatment on the filler A to obtain the biofilm carrier.
(2) Preparation of a pretreatment solution: 200mg of activated sludge and 5mg of bioflocculant are added into each 1L of LB culture medium, and the pretreated solution is transferred into a moving bed biofilm reactor after being uniformly mixed.
(3) Pretreatment: transferring the biomembrane carrier into a moving bed biomembrane reactor, fully contacting nitrobacteria with the filler through aeration, attaching the nitrobacteria to the surface of the adhesive layer of the filler to form a microorganism attachment layer, reacting for 3d at the water temperature of 10 ℃, stopping aeration, and discharging supernatant from the reactor to finish the pretreatment of the biomembrane carrier.
(4) Biofilm carrier initiation: introducing sewage with the water temperature of 6 ℃, the pH value of 7 +/-0.5 and the COD value of 700mg/L into the moving bed biofilm reactor until biofilm formation is completed, wherein the standard for evaluating the completion of the start of a biofilm carrier is that the COD value of effluent of the reactor is kept stable, a yellow biofilm is formed on the surface of a filler, protozoa and metazoa can be microscopically detected, and the time for completing the biofilm formation and the final COD value of the effluent are recorded.
The purification rate reflects the performance of the biological membrane in the biological sewage treatment process on the treatment of organic matters, and can be calculated by the following formula:
purification rate = (1-final effluent COD value/influent COD value) × 100%
TABLE 1 comparison of biofilm formation time and purification Rate for examples 1-7 and comparative examples 1-2
As shown in the data of Table 1, the average biofilm formation time of examples 1 to 7 was significantly reduced and the purification rate was significantly improved as compared with comparative examples 1 to 2, because the present invention obtained the biofilm carriers coated with the activating bacteria by immersing the biofilm carriers in the pretreatment solution containing the activating bacteria. Putting the pretreated biomembrane carrier into the sewage, enabling the activator to secrete NADH oxidase into a microenvironment on the surface of the biomembrane carrier, and activating a signal path on the microbial membrane after the microbes in the sewage are contacted with the NADH oxidase on the surface of the biomembrane carrier so as to promote the microbes to generate extracellular polysaccharide polymers and promote the microbes to be adhered to the surface of the biomembrane carrier; NADH oxidase enters the microbial cells and can promote the conversion of NADH in the microbial cells to NAD +, thereby switching the NADH oxidation pathway from the oxidative phosphorylation pathway for forming a large amount of ATP to the NADH oxidase pathway for generating water and ensuring the high NAD + level and the low ATP level required by high-speed glycolysis; microorganisms in wastewater readily absorb or produce peroxides such as H 2 O 2 Etc., which inhibit the proliferation of microorganisms, while NADH oxidase oxidizes peroxides to water inside cells to protect the microorganisms from the inhibition of peroxides. NADH oxygen secreted by pretreated biomembrane carrier-coated activated bacteriaThe chemozyme can promote the quick start of the biological membrane, and can also quickly start even under low temperature conditions.
Continuing with the data in table 1, the biofilm formation time of example 2 is further shortened compared to example 1, because the water temperature of the biofilm carrier pretreatment step and the water temperature of the wastewater in example 2 are proper temperatures for the growth of microorganisms, and the temperature difference is small, so that the microorganisms can adapt and grow well. Example 3 uses high concentration of the activator and can secrete more NADH oxidase, so the membrane hanging time is shorter than that of example 1. In example 4, since the biofilm carrier was not subjected to surface treatment and microorganisms were not easily attached, the biofilm formation time was longer than that in example 1. Example 1 compared to example 5, the pretreatment solution of example 1 is added with activated sludge, so as to increase the proportion of microorganisms in the biofilm carrier, which are beneficial to biofilm formation and purification, and further shorten the biofilm wall-hanging time and improve the purification efficiency. Example 1 compared to example 6, the addition of bioflocculant to the pretreatment solution of example 1 promoted attachment of microorganisms to the biofilm carrier, shortening the biofilm formation time. Example 7 start-up of biofilm carriers using wastewater with a COD value of 100mg/L resulted in longer biofilm formation time in example 7 than in other examples because of the lower COD value in the wastewater, but was still superior to the biofilm formation efficiency in comparative examples 1-2 because of the pretreatment step described above.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it is not limited to the embodiments, and those skilled in the art should understand that the technical solutions of the present invention can be modified or substituted with equivalents without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. The method for pretreating the biomembrane carrier is characterized by comprising the step of soaking the biomembrane carrier in a pretreatment solution containing activating bacteria, wherein the soaking condition is 10-20 ℃ for 1-3 d, the activating bacteria are strains secreting NADH oxidase, and the activating bacteria are at least one of bacillus and lactobacillus.
2. The pretreatment method for a biofilm carrier according to claim 1, wherein the amount of the activating bacteria added is 10 to 200mg/L.
3. The method for pretreating a biofilm carrier according to claim 1, wherein the biofilm carrier is subjected to surface modification treatment.
4. The pretreatment method for a biofilm carrier according to claim 3, wherein said surface modification treatment is at least one of a liquid-phase oxidation treatment, an ultrasonic treatment, a water bath treatment and a treatment with a polymer modifier.
5. The pretreatment method for a biofilm carrier according to claim 1, wherein said pretreatment solution contains a sewage treatment microorganism.
6. The method of claim 1, wherein the pretreatment solution comprises a bioflocculant.
7. A biological wastewater treatment process comprising the step of starting a biofilm carrier in wastewater, wherein the biofilm carrier is pretreated by the biofilm carrier pretreatment method according to any one of claims 1 to 6.
8. The biological wastewater treatment process according to claim 7, wherein the temperature of the wastewater is 6-38 ℃.
9. The biological sewage treatment process according to claim 7, wherein the COD value of the sewage is 100-700 mg/L.
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