CN111224142A - Novel microbial fuel cell generating device and assembling method thereof - Google Patents
Novel microbial fuel cell generating device and assembling method thereof Download PDFInfo
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- CN111224142A CN111224142A CN202010129612.6A CN202010129612A CN111224142A CN 111224142 A CN111224142 A CN 111224142A CN 202010129612 A CN202010129612 A CN 202010129612A CN 111224142 A CN111224142 A CN 111224142A
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- H—ELECTRICITY
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- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
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- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention belongs to the technical field of sewage treatment, and relates to a novel microbial fuel cell generating device and an assembly method thereof.A cell body sequentially comprises an anode cover plate, anode carbon cloth, an anode chamber, a proton membrane, a cathode chamber, cathode carbon cloth and a cathode cover plate which are fixedly connected, wherein liquid adding openings are respectively formed at the tops of the anode chamber and the cathode chamber, and an anode electrode contact piece and a cathode electrode contact piece are respectively and fixedly arranged at the bottoms of the anode cover plate and the cathode cover plate; the battery base comprises a battery base plate seat, a wiring seat, a battery clamping plate and a battery movable clamping plate which is hinged with a conductive spring between the battery clamping plate, a battery wiring terminal and a resistance wiring terminal are installed on the wiring seat, an anode electrode contact pin and a cathode electrode contact pin which are respectively contacted with an anode electrode contact piece and a cathode electrode contact piece are fixedly installed on the inner sides of the battery clamping plate and the battery movable clamping plate, and the problems that the liquid changing assembly of the fuel battery is complicated, the starting time of the fuel battery is long and the electricity generating capacity is low due to poor tightness of an anode chamber are solved.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and relates to a novel microbial fuel cell generating device and an assembling method thereof.
Background
With the acceleration of urbanization, the social demand for various resources is greatly increased, a large amount of pollutants are inevitably generated in production and life, the problem of environmental pollution is increasingly highlighted, and the problem of water pollution is particularly serious. According to statistics, in recent years, the discharge amount of wastewater in China is increased at a speed of 8% every year, and more than 80% of rivers and shallow groundwater in China are polluted to different degrees, so that the living environment of human beings is seriously influenced. The rapid development of social economy leads to the discharge of about 500 million tons of wastewater, mainly organic wastewater, in China every year. The organic waste water contains biochemical and non-biochemical organic pollutants, wherein the biochemical organic matters can consume a large amount of dissolved oxygen in the water body under the action of microorganisms to deteriorate the water quality, and the content of the biochemical organic matters reflects the important physicochemical properties of the waste water.
Microbial Fuel Cells (MFCs) are devices that convert low-grade chemical energy contained in wastewater into electrical energy. The device consists of a load microorganism (mainly electrogenesis bacteria), an anode and a cathode, and the working process can be summarized as follows: the anode organic matter generates proton and electron under the action of the oxidation decomposition of microbe, and the electron breathesEnzymes (NADH) with NAD+The proton in the electrolyte is transferred from the anode chamber to the cathode chamber under the drive of electric field force and concentration difference; electrons and protons are coupled to the electron acceptor (O) at the cathode2，Fe(CN)6 3-) A reduction reaction takes place.
The energy conversion of the organic matter in the MFC is divided into heat energy caused by entropy change and electric energy obtained through an external circuit load, and the larger the external circuit load is, the higher the energy that can be obtained. As the external circuit load approaches infinity, the energy of the MFC is equivalent to the free energy available in standard cell electromotive force. However, it is difficult to achieve the standard electromotive force of MFC in fact because there is a large energy loss during the electrode reaction and mass transfer process, mainly including activation overpotential, concentration overpotential, and ohmic overpotential. The activation overpotential represents the activation energy consumed for electrochemical reactions (anodic oxidation, cathodic reduction processes) to occur at the electrode surface. The activation overpotential can be reduced from several aspects: the temperature can ensure higher microbial activity to reduce activation energy by regulating and controlling the living environment of the microbes, including pH; g.sulfurfureducated microorganisms and s.oneidensis in the anode electrogenesis microorganisms are two main extracellular electrogenesis flora, which can directly transfer electrons to a cathode through cytochrome on an outer layer membrane of the microorganisms and can also transfer the electrons by using an electron mediator generated by self metabolism, and the external electron mediator can reduce the energy barrier of the electrons between the microorganisms and electrodes so as to improve the effective transfer of the electrons; the physical properties of the anode, the cathode itself (roughness, specific surface area, conductivity, etc.), and the cathode oxygen reduction catalyst and its loading are also important factors affecting the activation overpotential. The concentration overpotential is concentration polarization caused by concentration limitation of reactants when the anodic oxidation rate is greater than the diffusion rate of organic matters or the cathodic oxygen diffusion rate is less than the oxygen reduction rate, mainly related to the biofilm on the electrode surface, the water conservancy condition and the configuration design of a reactor, and can reduce the concentration polarization by increasing the contact area of electrode materials and reactants or arranging a stirring device. The ohmic overpotential is determined by the proton transfer resistance, and the main influence factors are the size, thickness and pore diameter of the membrane, the conductivity of the electrolyte, the electrode spacing and the like, the ohmic impedance is in positive linear correlation with the current density, and generally the ohmic loss can be reduced by reducing the electrode spacing or increasing the conductivity.
However, the existing microbial fuel cell is very complicated in liquid change and assembly, and the anode chamber is not well sealed, the main reasons are that the cathode and the anode of the existing microbial fuel cell are conducted through a lead and voltage acquisition is carried out by using an electrode clamp, the lead extends out of the cell from the cavity of the cathode and the anode, the cell is not hermetically mounted, the contact of the electrode clamp is not very tight, after long-time operation, the lead and the electrode clamp are corroded, the resistance is increased, the acquired data are inaccurate, the starting time of the cell is prolonged, the anaerobic environment of the anode chamber is poor, the activity of anaerobic electrogenesis bacteria is low due to aerobic bacteria enrichment of the anode, the electrogenesis capacity of the microbial fuel cell is further reduced, the culture time is long, the performance is unstable, the bacteria are easy to generate, and the research efficiency is low. The traditional microbial fuel cell seriously restricts the deep development of the MFC in the scientific research field of water pollution control and water environment management for a long time.
Aiming at the hysteresis of the traditional microbial fuel cell, an anaerobic environment which can ensure the simplicity and convenience of the whole liquid changing assembly process and can ensure an anode chamber is needed to be designed; the microbial fuel cell design firstly solves the problems that the whole cell liquid changing assembly process is simple and convenient, the anode anaerobic environment is guaranteed, the constancy of an external resistor is guaranteed, the liquid leakage phenomenon of the traditional microbial fuel cell is solved, the problems that the traditional microbial fuel cell is long in starting time, the anode chamber anaerobic environment is poor, the activity of anaerobic electricity generating bacteria is low, the electricity generating capacity of the cell is reduced, the performance is unstable, the mixed bacteria are easy to generate, the scientific research efficiency is low and the like are solved, and the microbial fuel cell design has important significance for promoting water pollution control and water environment management scientific research.
Disclosure of Invention
In view of the above, the invention provides a novel microbial fuel cell generating device and an assembling method thereof, in order to solve the problems that the microbial fuel cell has a complicated liquid changing and assembling process and poor tightness of an anode chamber, so that the microbial fuel cell has long starting time and low power generation capacity, and the development of the microbial fuel cell in the field of water pollution control is restricted.
In order to achieve the above object, the present invention provides a novel microbial fuel cell generator, which comprises a cylindrical cell body and a rectangular cell base, wherein the cell body and the rectangular cell base are used in cooperation;
the cell body comprises an anode chamber and a cathode chamber which are matched with each other and are clamped with a proton membrane in the middle, the outer sides of the anode chamber and the cathode chamber are respectively and fixedly provided with an anode cover plate and a cathode cover plate, anode carbon cloth is clamped between the anode cover plate and the anode chamber, cathode carbon cloth is clamped between the cathode chamber and the cathode cover plate, the middle of the cathode cover plate is provided with an inner chamber communicated with the cathode chamber, the tops of the anode chamber and the cathode chamber are respectively provided with an anode chamber liquid feeding port and a cathode chamber liquid feeding port communicated with the inner chamber of the cell body, and the bottoms of the;
the battery base comprises a battery base plate seat, a plurality of wiring seats which are uniformly and fixedly installed on one side of the battery base plate seat, a plurality of battery clamping plates which are uniformly and fixedly installed on the battery base plate seat and a battery movable clamping plate which is movably installed on the battery base plate seat and corresponds to the battery clamping plates, a through hole which is convenient for liquid to pass through is formed in the lower portion of each battery clamping plate, a plurality of conductive springs are hinged between each battery clamping plate and the corresponding battery movable clamping plate, a battery wiring terminal and a resistance wiring terminal are fixedly installed on each wiring seat, and an anode electrode contact pin which is in contact with an anode electrode contact piece and a cathode electrode contact pin which is in contact with a cathode electrode contact piece are fixedly installed on the inner.
Further, anode chamber filling opening and cathode chamber filling opening are the circular port of in-band screw thread, and threaded connection has anode chamber filling channel and cathode chamber filling channel respectively on anode chamber filling opening and the cathode chamber filling opening, but on anode chamber filling channel and the cathode chamber filling channel respectively demountable installation have anode chamber filling cap and cathode chamber filling cap.
Further, positive pole apron and anode chamber are fixed through positive pole screw I and positive pole screw II that are located positive pole apron four corners, and positive pole carbon cloth middle part fixed mounting has positive pole electrode nut, and positive pole apron middle part is worn to establish positive pole screw III that uses with the cooperation of positive pole electrode nut, and negative pole apron and cathode chamber are fixed through negative pole screw I and negative pole screw II that are located negative pole apron four corners.
Further, the battery terminals include a battery anode terminal and a battery cathode terminal, and the resistive terminals include a resistive positive terminal and a resistive negative terminal.
Furthermore, a battery fixing frame is fixedly arranged on a battery base plate at the bottom of the battery clamping plate, and the battery movable clamping plate slides along the battery fixing frame.
Furthermore, the anode electrode contact piece and the cathode electrode contact piece are both made of copper sheet gold-plated materials, the interior of the anode electrode contact piece is in contact with the anode screw III, and the interior of the cathode electrode contact piece is in contact with the cathode carbon cloth.
Furthermore, the anode chamber liquid feeding channel and the cathode chamber liquid feeding channel are both a luer female connector made of pp materials with the inner diameter of 3mm, and the anode chamber liquid feeding cap and the cathode chamber liquid feeding cap are both luer male plugs.
Furthermore, the battery wiring terminal and the resistance wiring terminal are in a parallel structure.
Further, the battery anode terminal is connected with the anode electrode contact pin, and the battery cathode terminal is connected with the conductive spring.
The assembling method of the novel microbial fuel cell generating device comprises the following steps:
A. the anode cover plate, the anode chamber, the cathode chamber and the cathode cover plate are sequentially placed, an anode carbon cloth with a hole in the middle is inserted between the anode cover plate and the anode chamber, an anode screw III penetrating through the anode cover plate fixes the anode carbon cloth, anode screws I and anode screws II at four corners of the anode cover plate fix the anode cover plate, the anode chamber and the proton membrane on the cathode chamber, and cathode screws I and cathode screws II at four corners of the cathode cover plate fix the cathode cover plate and the cathode carbon cloth on the cathode chamber;
B. screwing the anode chamber liquid adding channel into the anode chamber liquid adding port, covering the anode chamber liquid adding cap on the anode chamber liquid adding channel, screwing the cathode chamber liquid adding channel into the cathode chamber liquid adding port, and covering the cathode chamber liquid adding cap on the cathode chamber liquid adding channel;
C. connecting a battery anode terminal of a battery terminal to a voltage acquisition cathode, connecting a battery cathode terminal to a voltage acquisition anode, pulling a battery movable clamping plate open, and connecting a resistor between a resistor terminal of a resistor terminal and a resistor cathode terminal;
D. opening an anode chamber liquid adding cap of the novel microbial fuel cell, adding anolyte or actual wastewater to be measured, covering the anode chamber liquid adding cap, then opening a cathode chamber liquid adding cap of the novel microbial fuel cell, adding cathode buffer solution, and covering the cathode chamber liquid adding cap;
E. the movable battery clamping plate is pulled open, the novel microbial fuel cell body is arranged between the movable battery clamping plate and the battery fixing frame, the anode electrode contact piece is contacted with the anode electrode contact pin, and data acquisition and microbial stabilized voltage calculation are carried out after the cathode electrode contact piece is contacted with the cathode electrode contact pin.
The invention has the beneficial effects that:
1. the novel microbial fuel cell generating device disclosed by the invention is characterized in that the cell clamp plate is fixedly arranged on the cell base, and the cell clamp plate is connected with the cell movable clamp plate through the conductive spring, so that the cell body can be easily and quickly arranged between the cell movable clamp plate and the cell clamp plate, the whole novel microbial cell liquid changing process is simpler and more convenient, the anode chamber and the anode cover plate are fixed through the anode screw, the anode liquid is added through the anode chamber liquid adding channel, and the anode chamber liquid adding channel is sealed through the anode chamber liquid adding cap, so that the anaerobic environment of the anode chamber is ensured.
2. The invention discloses a novel microbial fuel cell generating device, wherein a cell anode wiring end of a cell wiring terminal is connected with a voltage acquisition cathode, a cell cathode wiring end is connected with a voltage acquisition anode, a cell movable clamping plate is pulled open, a resistor is connected between a resistor wiring end and a resistor cathode wiring end of the resistor wiring terminal, the resistor is used for enabling a cell to be in a closed loop, current exists in the loop, so that a voltage collector can collect voltage, and when the voltage is collected by using a common voltage collector, the resistor is required to be connected, so that the loop can be formed to generate current and measure voltage. If the BOD-Q water quality tester with the welded resistance is used for collecting the voltage and calculating the BOD, the resistance is not required to be connected. The anode carbon cloth of the novel microbial fuel cell generating device disclosed by the invention is fixed on the anode cover plate by screws, conducts electricity through an internal circuit board and does not have an exposed conductive wire, so that the growth of the anode carbon cloth and the electricity-producing microorganisms on the anode carbon cloth cannot be damaged in the liquid changing process, and the anode electricity-producing microorganisms can be promoted to rapidly grow on the anode carbon cloth and reach a peak value; furthermore, because the novel microbial fuel cell generating device disclosed by the invention is sealed by the annular sealing ring and the silica gel gasket in a double way, and the luer joint is added, the generating device can perform good sealing function at the liquid changing port, so that the tightness of the microbial fuel cell is very good, a very good anaerobic environment is provided for electrogenesis anaerobic bacteria in the anode chamber, the rapid growth of electrogenesis microorganisms is ensured, the novel microbial fuel cell generating device disclosed by the invention is provided with an independent resistance access terminal, the accuracy of the resistance is ensured, unlike traditional microbial fuel cell, which connects the resistor to two ends of the microbial fuel cell and exposes many wires, the connection method is easy to corrode the resistor and cause the resistor to become larger, therefore, the voltage sampling value is influenced, and the starting time of the microbial cell can be prolonged by optimizing and integrating various items of the novel microbial fuel cell generating device disclosed by the invention.
3. The novel microbial fuel cell generating device disclosed by the invention has the advantages that the service life is longer than 12 months, the starting time of the microbial fuel cell is shorter than 10 days, the voltage acquisition stability is over 99.5%, and the coulomb quantity of the actual wastewater anode and the standard sample with the concentration detection range (compared with the traditional BOD index) of 2-100mg L-1 are compared. When the BOD standard sample is measured, the accuracy is not less than 97%, and when the actual wastewater is measured, the accuracy is not less than 96%.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Drawings
For the purposes of promoting a better understanding of the objects, aspects and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic structural diagram of a novel microbial fuel cell generator of the present invention;
FIG. 2 is a side view of the cathode of the cell body of the novel microbial fuel cell generator of the present invention;
FIG. 3 is a side view of the anode of the cell body of the novel microbial fuel cell generator of the present invention;
FIG. 4 is a cross-sectional view of a cell body of the novel microbial fuel cell generator of the present invention;
FIG. 5 is a top view of a cell base of the novel microbial fuel cell generator of the present invention;
FIG. 6 is a graph of voltage versus time trend for acclimatization of microorganisms using the novel microbial fuel cell generating apparatus of the present invention;
FIG. 7 is a graph of voltage versus time for a BOD of 200mg/L standard using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 8 is a graph of voltage versus time for a BOD of 100mg/L standard using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 9 is a graph of voltage versus time for a BOD of 50mg/L standard using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 10 is a graph of voltage versus time for a BOD of 25mg/L standard using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 11 is a graph of voltage versus time for river water measurements using the novel microbial fuel cell generator of the present invention;
FIG. 12 is a graph showing voltage-time curves of domestic sewage measured by the novel microbial fuel cell generator of the present invention;
FIG. 13 is a graph of voltage versus time for measuring landfill leachate using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 14 is a graph of voltage versus time for the measurement of pharmaceutical wastewater using the novel microbial fuel cell generation apparatus of the present invention;
FIG. 15 is a graph of stabilized voltage versus time output when a signal is provided by a standard voltage of 500mv for a novel microbial fuel cell generator of the present invention.
Reference numerals: an anode cover plate 1, an anode chamber 2, a cathode chamber 3, a cathode cover plate 4, a cathode screw I5, a cathode screw II 6, a cathode electrode contact piece 7, an anode screw I8, an anode screw II 9, an anode screw III 10, an anode electrode contact piece 11, a cathode chamber liquid adding cap 12, a cathode chamber liquid adding channel 13, an anode chamber liquid adding cap 14, an anode chamber liquid adding channel 15, an anode electrode nut 16, anode carbon cloth 17 and a proton membrane 18, the device comprises a cathode carbon cloth 19, a battery base 20, a resistance negative terminal 21, a resistance positive terminal 22, a battery positive terminal 23, a battery negative terminal 24, a battery clamping plate 25, a positive electrode contact pin 26, a battery fixing frame 27, a conductive spring 28, a negative electrode contact pin 29, a battery movable clamping plate 30, a fixing screw hole 31, a wire holder 32, a battery connecting terminal 33, a resistance connecting terminal 34, an anode chamber liquid filling opening 35 and a cathode chamber liquid filling opening 36.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention in a schematic way, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in the drawings in which there is no intention to limit the invention thereto; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "front", "rear", etc., based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not an indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes, and are not to be construed as limiting the present invention, and the specific meaning of the terms may be understood by those skilled in the art according to specific situations.
The novel microbial fuel cell generating device shown in fig. 1-5 comprises a cell body and a rectangular cell base 20 which are used in cooperation, wherein the inner cavities of the cell body and the cell base 20 are cylindrical, and the cell body and the cell base 20 are made of organic glass materials; two fixing screw holes 31 are respectively formed at two ends of the battery base 20, so that the battery base 20 can be conveniently fixed.
The cell body mainly comprises an anode cover plate 1, an anode chamber 2, a cathode chamber 3 and a cathode cover plate 4 which are made of organic glass, and a silica gel sheet is arranged between the anode cover plate 1 and the anode chamber 2 and used for preventing liquid leakage. A silica gel sheet is arranged between the cathode cover plate 4 and the cathode chamber 3 for preventing liquid leakage. A proton membrane 18 is clamped between the anode chamber 2 and the cathode chamber 3, and a silica gel sheet is arranged between the cathode chamber 3 and the proton membrane 18 for preventing liquid leakage. The anode cover plate 1 and the cathode cover plate 4 are respectively and fixedly arranged at the outer sides of the anode chamber 2 and the cathode chamber 3, the anode carbon cloth 17 is clamped between the anode cover plate 1 and the anode chamber 2, and the cathode carbon cloth 19 is clamped between the cathode chamber 3 and the cathode cover plate 4. Anode cover plate 1, anode chamber 2 and cathode chamber 3 are fixed through 8 positive pole screws I8 and 4 positive pole screws II 9 that are located 1 four corners of positive pole apron, positive pole carbon cloth 17 middle part fixed mounting has positive pole electrode nut 16, positive pole electrode nut 16 is M4 hexagonal 304 stainless steel nut, open the internal thread hole that has M4 in 1 middle part of the positive pole apron, wear to establish in this internal thread hole and cooperate the positive pole screw III 10 that uses with positive pole electrode nut 16, realize positive pole apron 1 through positive pole screw III 10, positive pole carbon cloth 17 and anode chamber 2's fixed. An anode cover plate 1, an anode chamber 2, a proton membrane 18 and a silica gel sheet are fixed on the cathode chamber 3 through an anode screw I and an anode screw II. Cathode apron 4 and cathode chamber 3 are fixed through 8 cathode screws I and 4 cathode screws II that are located 4 four corners of cathode apron, through cathode screw I5 and cathode screw II 6 with cathode apron 4, negative pole carbon cloth 19 and silica gel piece with fix on cathode chamber 3. The anode screw I is an M4 hexagon socket 304 stainless steel countersunk head screw; the anode screw II is an M3 cross 304 stainless steel countersunk head screw; the anode screw III is an M4 cross 304 stainless steel countersunk head screw. The cathode screw I is an M3 cross 304 stainless steel countersunk head screw; the cathode screw II is an M4 cross 304 stainless steel countersunk head screw.
There is the inner chamber that communicates with each other with cathode chamber 3 in the middle of the negative pole apron 4, anode chamber 35 and the communicating anode chamber filling opening 36 of cell body inner chamber are seted up respectively to anode chamber 2 and 3 tops of cathode chamber, anode chamber filling opening 35 and cathode chamber filling opening 36 are the circular port of internal screw thread, there are anode chamber filling channel 15 and cathode chamber filling channel 13 on anode chamber filling opening 35 and the cathode chamber filling opening 36 threaded connection respectively, there are anode chamber filling cap 14 and cathode chamber filling cap 12 on anode chamber filling channel 15 and the cathode chamber filling channel 13 demountable installation respectively. The anode chamber liquid feeding channel 15 and the cathode chamber liquid feeding channel 13 are both made of pp connectors with the inner diameter of 3mm, and the anode chamber 2 liquid feeding cap and the cathode chamber 3 liquid feeding cap are both made of luer plugs. The bottom parts of the anode cover plate 1 and the cathode cover plate 4 are respectively and fixedly provided with an anode electrode contact piece 11 and a cathode electrode contact piece 7; the anode electrode contact piece 11 and the cathode electrode contact piece 7 are both made of copper sheet gold-plated materials, the interior of the anode electrode contact piece 11 is in contact with an anode screw III, and the interior of the cathode electrode contact piece 7 is in contact with a cathode carbon cloth 19.
The battery base comprises a battery base plate seat, four wiring seats 32 which are uniformly and fixedly arranged on one side of the battery base plate seat, four battery clamping plates 25 which are uniformly and fixedly arranged on the battery base plate seat and a battery movable clamping plate 30 which is movably arranged on the battery base plate seat and corresponds to the battery clamping plates 25, a through hole which is convenient for liquid to pass through is formed in the lower part of each battery clamping plate 25, a battery fixing frame 27 is fixedly arranged on a battery base plate 20 at the bottom of each battery clamping plate 25, and the battery movable clamping plate 30 slides along the battery fixing frame 27. A plurality of conductive springs 28 are hinged between the battery clamping plate 25 and the corresponding battery movable clamping plate 30, a battery wiring terminal 33 and a resistance wiring terminal 34 are fixedly mounted on each wiring seat 32, and the battery wiring terminal 33 and the resistance wiring terminal 34 are in a parallel structure. Cell terminals 33 include cell anode terminals 23 and cell cathode terminals 24, and resistive terminals 34 include resistive positive terminals 22 and resistive negative terminals 21. The cell anode terminal 23 is in communication with an anode electrode contact pin 26 and the cell cathode terminal 24 is in communication with a conductive spring 28. An anode electrode contact pin 26 contacting the anode electrode contact piece 11 and a cathode electrode contact pin 29 contacting the cathode electrode contact piece 7 are fixedly mounted on the inner sides of the battery clamping plate 25 and the battery movable clamping plate 30, respectively.
The assembling method of the novel microbial fuel cell generating device comprises the following steps:
A. placing an anode cover plate 1, an anode chamber 2, a cathode chamber 3 and a cathode cover plate 4 in sequence, cutting an anode carbon cloth 17 into a circle with the diameter of 3cm, punching a small hole with the diameter of 4mm in the middle, inserting the anode carbon cloth 17 punched in the middle between the anode cover plate 1 and the anode chamber 2, fixing the anode carbon cloth 17 by an anode screw III penetrating on the anode cover plate 1, fixing the anode cover plate 1, a silica gel gasket, the anode chamber 2, a proton membrane 18 and the silica gel gasket on the cathode chamber 3 by anode screws I and anode screws II at four corners of the anode cover plate 1, and fixing the cathode cover plate 4, a cathode carbon cloth 19 and the silica gel gasket on the cathode chamber 3 by cathode screws I and cathode screws II at four corners of the cathode cover plate 4;
B. screwing the anode chamber liquid adding channel 15 into the anode chamber liquid adding port 35, covering the anode chamber liquid adding cap 14 on the anode chamber liquid adding channel 15, screwing the cathode chamber liquid adding channel 13 into the cathode chamber liquid adding port 36, and covering the cathode chamber liquid adding cap 12 on the cathode chamber liquid adding channel 13;
C. connecting a battery anode terminal 23 of a battery terminal 33 with a voltage acquisition cathode of a BOD-Q water quality tester, connecting a battery cathode terminal 24 with a voltage acquisition anode of the BOD-Q water quality tester, pulling a battery movable clamping plate 30 open, and connecting a resistance of 1000 ohms between a resistance terminal of a resistance terminal 34 and a resistance cathode terminal 21;
D. opening an anode chamber liquid adding cap 14 of the novel microbial fuel cell, adding anolyte or actual wastewater to be measured, covering the anode chamber liquid adding cap 14, then opening a cathode chamber liquid adding cap 12 of the novel microbial fuel cell, adding cathode buffer solution, and covering the cathode chamber liquid adding cap 12;
E. the battery movable clamping plate 30 is pulled open, the novel microbial fuel cell body is arranged between the battery movable clamping plate 30 and the battery fixing frame 27, the anode electrode contact piece 11 is contacted with the anode electrode contact pin 26, and the cathode electrode contact piece 7 is contacted with the cathode electrode contact pin 29 for data acquisition and calculation of microbial stable voltage.
Fig. 6 is a voltage-time trend graph of the microorganism acclimation by using the novel microbial fuel cell generation device of the present invention, the time for acclimating the microorganism by using the microbial fuel cell assembled by using the novel microbial fuel cell device of the present invention and the assembly method thereof can be reduced, the time for acclimating the microorganism by using the conventional microbial fuel cell requires more than 30 days, and the time for selecting the microorganism by using the novel microbial fuel cell device of the present invention and the assembly method thereof only requires 10 days, which shortens the time by more than 66%.
FIG. 7 is a voltage-time graph of BOD of 200mg/L standard solution measured by the novel microbial fuel cell generator of the present invention, wherein the BOD is the amount of dissolved oxygen consumed by microbial metabolism.
The BOD (mg/L) test value of the solution was calculated by the following formula:
wherein F is the Faraday constant, 96485C/mol; vAnIs the effective volume of the anode chamber, mL; ecellIs the MFC output voltage, mV; rextIs an external circuit load, Ω.
BOD was found to be 202.3mg/L with an accuracy of 98.86%.
FIG. 8 is a graph of voltage versus time for a BOD of 100mg/L standard solution using the novel microbial fuel cell generator of the present invention. Wherein BOD value is dissolved oxygen consumed by microorganism metabolism, measured BOD is 97.4mg/L, and accuracy is 97.4%.
FIG. 9 is a graph of voltage versus time for a BOD of 50mg/L standard solution using the novel microbial fuel cell generator of the present invention. Wherein the BOD value is dissolved oxygen consumed by microbial metabolism, and the measured BOD is 49.70mg/L with the accuracy of 99.40%.
FIG. 10 is a graph of voltage versus time for a BOD of 25mg/L standard solution using the novel microbial fuel cell generator of the present invention. Wherein BOD value is dissolved oxygen consumed by microorganism metabolism, measured BOD is 24.60mg/L, and accuracy is 98.40%.
FIG. 11 is a voltage-time curve diagram of river water measurement using the novel microbial fuel cell generator of the present invention, in which BOD is the dissolved oxygen consumed by microbial metabolism, water sample is diluted 2 times, measured BOD is 29.80mg/L, and measured BOD is measured by the national standard method530.01mg/L with an accuracy of 99.30%.
FIG. 12 is a voltage-time graph of domestic sewage measured by the novel microbial fuel cell generator of the present invention, in which the BOD value is the dissolved oxygen amount consumed by microbial metabolism, the water sample is diluted 2 times, the measured BOD is 49.00mg/L, and the measured BOD is measured by the national standard method5It was 48.13mg/L with an accuracy of 98.22%.
FIG. 13 is a graph of voltage-time curve of landfill leachate measured by using the novel microbial fuel cell generator of the present invention, in which BOD is dissolved oxygen consumed by microbial metabolism, water sample is diluted 3 times, measured BOD is 106.89mg/L, and BO is measured by the national standard methodD5103.36mg/L with an accuracy of 96.69%.
FIG. 14 is a voltage-time curve diagram of the pharmaceutical wastewater determination by using the novel microbial fuel cell generation device of the present invention, wherein the BOD value is the dissolved oxygen amount consumed by the microbial metabolism, the water sample is diluted 2 times, the measured BOD is 22.80mg/L, and the BOD is measured by the national standard method522.56mg/L with an accuracy of 98.95%.
Fig. 15 is a graph of stabilized voltage-time outputted when the novel microbial fuel cell generator of the present invention provides signals with 500mv standard voltage, and it can be seen that when the stabilized voltage signal is 500mv, the voltage collector monitors data for one month, which shows that the voltage signals outputted by the microbial fuel cell assembled by the novel microbial fuel cell generator and the assembling method thereof of the present invention are very stable, and the degree of stability reaches more than 99.50%.
Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.
Claims (10)
1. A novel microbial fuel cell generating device is characterized by comprising a cell body and a cuboid cell base, wherein the cell body and the cell base are mutually matched for use, and the inner cavity of the cell body is cylindrical;
the cell body comprises an anode chamber and a cathode chamber which are matched with each other and are clamped with a proton membrane in the middle, the outer sides of the anode chamber and the cathode chamber are respectively and fixedly provided with an anode cover plate and a cathode cover plate, anode carbon cloth is clamped between the anode cover plate and the anode chamber, cathode carbon cloth is clamped between the cathode chamber and the cathode cover plate, an inner chamber communicated with the cathode chamber is arranged in the middle of the cathode cover plate, the top parts of the anode chamber and the cathode chamber are respectively provided with an anode chamber liquid feeding port and a cathode chamber liquid feeding port communicated with the inner chamber of the cell body, and the bottom parts of;
the battery base comprises a battery base plate seat, a plurality of wiring seats, a plurality of battery clamping plates and a battery movable clamping plate, wherein the wiring seats are uniformly and fixedly arranged on one side of the battery base plate seat, the battery clamping plates are uniformly and fixedly arranged on the battery base plate seat, the battery movable clamping plates are corresponding to the battery clamping plates, a through hole convenient for liquid to pass through is formed in the lower portions of the battery clamping plates, a plurality of conductive springs are hinged between the battery clamping plates and the corresponding battery movable clamping plates, a battery wiring terminal and a resistance wiring terminal are fixedly arranged on each wiring seat, and an anode electrode contact pin and a cathode electrode contact pin are fixedly arranged on the inner sides of the battery clamping plates and the inner sides of the battery movable clamping plates respectively, the.
2. The novel microbial fuel cell generator of claim 1, wherein the anode chamber filler opening and the cathode chamber filler opening are circular holes with internal threads, the anode chamber filler opening and the cathode chamber filler opening are respectively in threaded connection with an anode chamber filler channel and a cathode chamber filler channel, and the anode chamber filler channel and the cathode chamber filler channel are respectively detachably provided with an anode chamber filler cap and a cathode chamber filler cap.
3. The novel microbial fuel cell generator according to claim 2, wherein the anode cover plate and the anode chamber are fixed by anode screws i and ii at four corners of the anode cover plate, an anode electrode nut is fixedly mounted in the middle of the anode carbon cloth, an anode screw iii used in cooperation with the anode electrode nut is inserted in the middle of the anode cover plate, and the cathode cover plate and the cathode chamber are fixed by cathode screws i and ii at four corners of the cathode cover plate.
4. The novel microbial fuel cell generator of claim 1 wherein said cell terminals comprise a cell anode terminal and a cell cathode terminal and said resistive terminals comprise a resistive positive terminal and a resistive negative terminal.
5. The novel microbial fuel cell generator of claim 4, wherein the battery holder is fixedly mounted on the battery base plate at the bottom of the battery clamping plate, and the battery movable clamping plate slides along the battery holder.
6. The novel microbial fuel cell generator of claim 3, wherein the anode contact piece and the cathode contact piece are made of gold-plated copper sheets, the inside of the anode contact piece is in contact with the anode screw III, and the inside of the cathode contact piece is in contact with the cathode carbon cloth.
7. The novel microbial fuel cell generating device of claim 2, wherein the anode chamber charging channel and the cathode chamber charging channel are both luer female connectors with 3mm inner diameter, and the anode chamber charging cap and the cathode chamber charging cap are both luer male plugs.
8. The microbial fuel cell generator of claim 5 wherein said cell terminals and said resistive terminals are in a parallel configuration.
9. The novel microbial fuel cell generator of claim 8 wherein said cell anode terminal is in communication with an anode electrode contact pin and said cell cathode terminal is in communication with a conductive spring.
10. The assembling method of the novel microbial fuel cell generating device is characterized by comprising the following steps:
A. the anode cover plate, the anode chamber, the cathode chamber and the cathode cover plate are sequentially placed, an anode carbon cloth with a hole in the middle is inserted between the anode cover plate and the anode chamber, an anode screw III penetrating through the anode cover plate fixes the anode carbon cloth, anode screws I and anode screws II at four corners of the anode cover plate fix the anode cover plate, the anode chamber and the proton membrane on the cathode chamber, and cathode screws I and cathode screws II at four corners of the cathode cover plate fix the cathode cover plate and the cathode carbon cloth on the cathode chamber;
B. screwing the anode chamber liquid adding channel into the anode chamber liquid adding port, covering the anode chamber liquid adding cap on the anode chamber liquid adding channel, screwing the cathode chamber liquid adding channel into the cathode chamber liquid adding port, and covering the cathode chamber liquid adding cap on the cathode chamber liquid adding channel;
C. connecting a battery anode terminal of a battery terminal to a voltage acquisition cathode, connecting a battery cathode terminal to a voltage acquisition anode, pulling a battery movable clamping plate open, and connecting a resistor between a resistor terminal of a resistor terminal and a resistor cathode terminal;
D. opening an anode chamber liquid adding cap of the novel microbial fuel cell, adding anolyte or actual wastewater to be measured, covering the anode chamber liquid adding cap, then opening a cathode chamber liquid adding cap of the novel microbial fuel cell, adding cathode buffer solution, and covering the cathode chamber liquid adding cap;
E. the movable battery clamping plate is pulled open, the novel microbial fuel cell body is arranged between the movable battery clamping plate and the battery fixing frame, the anode electrode contact piece is contacted with the anode electrode contact pin, and data acquisition and microbial stabilized voltage calculation are carried out after the cathode electrode contact piece is contacted with the cathode electrode contact pin.
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| CN202010129612.6A CN111224142A (en) | 2020-02-28 | 2020-02-28 | Novel microbial fuel cell generating device and assembling method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112960780A (en) * | 2021-03-03 | 2021-06-15 | 龙江环保集团股份有限公司 | Pretreatment method of biomembrane carrier and biological sewage treatment process |
| CN113740402A (en) * | 2021-07-27 | 2021-12-03 | 中国科学院重庆绿色智能技术研究院 | BOD measures intelligent sensor |
| CN115340969A (en) * | 2022-09-20 | 2022-11-15 | 中国科学院重庆绿色智能技术研究院 | Flora domestication method and application of BOD (biochemical oxygen demand) microbial electrochemical sensor |
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2020
- 2020-02-28 CN CN202010129612.6A patent/CN111224142A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112960780A (en) * | 2021-03-03 | 2021-06-15 | 龙江环保集团股份有限公司 | Pretreatment method of biomembrane carrier and biological sewage treatment process |
| CN112960780B (en) * | 2021-03-03 | 2023-02-03 | 龙江环保集团股份有限公司 | Pretreatment method of biomembrane carrier and biological sewage treatment process |
| CN113740402A (en) * | 2021-07-27 | 2021-12-03 | 中国科学院重庆绿色智能技术研究院 | BOD measures intelligent sensor |
| CN113740402B (en) * | 2021-07-27 | 2024-05-28 | 中国科学院重庆绿色智能技术研究院 | BOD measures intelligent sensor |
| CN115340969A (en) * | 2022-09-20 | 2022-11-15 | 中国科学院重庆绿色智能技术研究院 | Flora domestication method and application of BOD (biochemical oxygen demand) microbial electrochemical sensor |
| CN115340969B (en) * | 2022-09-20 | 2024-01-30 | 中国科学院重庆绿色智能技术研究院 | Flora domestication method and application of BOD microbial electrochemical sensor |
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