CN112945806A - Transdermal diffusion cell, transdermal diffusion instrument and oral mucosa transdermal diffusion experimental method - Google Patents
Transdermal diffusion cell, transdermal diffusion instrument and oral mucosa transdermal diffusion experimental method Download PDFInfo
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- CN112945806A CN112945806A CN202110138343.4A CN202110138343A CN112945806A CN 112945806 A CN112945806 A CN 112945806A CN 202110138343 A CN202110138343 A CN 202110138343A CN 112945806 A CN112945806 A CN 112945806A
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- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
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Abstract
The invention discloses a transdermal diffusion cell, a transdermal diffusion instrument and an oral mucosa transdermal diffusion experiment method, and belongs to the technical field of transdermal experiments. The pond upper end of dosing of diffusion cell is provided with agitating unit, agitating unit includes: the device comprises a base, a first power source, a first transmission unit, a second transmission unit, a lifting platform, a stirring unit and a control module; the second transmission unit comprises a driving gear and at least two driven gears which are arranged around the periphery of the driving gear and meshed with the driving gear, and the driven gears are provided with screw rods and can drive the lifting platform to move up and down; the stirring unit comprises a second power source and a stirring rod, and one end of the stirring rod is provided with a stirring paddle located in the dosing pool. The invention can enable the environment in the diffusion cell to be close to the environment in the oral cavity during gargling through stirring, thereby effectively carrying out the oral mucosa transdermal diffusion experiment, enabling the experimental result to be more accurate, and further providing the transdermal diffusion instrument and the oral mucosa transdermal diffusion experimental method.
Description
Technical Field
The invention belongs to the technical field of transdermal experiments, and particularly relates to a transdermal diffusion cell, a transdermal diffusion instrument and an oral mucosa transdermal diffusion experiment method.
Background
The experimental method is that the skin to be tested is clamped in a diffusion cell, then the diffusion cell is placed in a transdermal diffusion device, the drug to be tested on the upper side of the skin permeates the skin of the diffusion cell and is soaked in physiological saline on the lower side of the skin in a heating mode of the diffusion cell, the concentration of a detection solvent is periodically extracted, the amount of the drug permeating the skin is analyzed, experimental data recording is carried out, and finally average data is obtained through multiple times of evaluation.
At present, when the transdermal diffusion experiment, often adopt the Franz diffusion cell, the structure of this type of diffusion cell is shown in figure 1, including receiving reservoir and dosing pool, when carrying out the diffusion experiment, the dosing pool is detained on the receiving reservoir to dosing pool and receiving reservoir intercommunication are provided with the thief hole on the receiving reservoir, the convenient sample, and the top intercommunication atmosphere of dosing pool, membrane or skin clamp for the experiment are between receiving reservoir and dosing pool, then adopt the pipe clamp to clip. However, in the transdermal diffusion experiment, there is an experiment to simulate the transdermal diffusion process during gargling, and it is known from life experience that in the process of gargling, the mouthwash continuously rolls in the mouth, and the mouthwash has a certain impact force on the oral mucosa, and because the transdermal diffusion experiment is in vitro simulation, when the transdermal diffusion of the oral mucosa is simulated in vitro, more factors need to be considered, for example, how to ensure that the water flow can stably impact the mucosa, and prevent the mucosa from being damaged. In the experimental process, the better experimental effect is difficult to achieve when the transdermal diffusion process of the oral mucosa is simulated by using the existing diffusion cell during the gargling process.
Disclosure of Invention
The technical problem is as follows: the invention provides a transdermal diffusion cell aiming at the problem that the existing transdermal diffusion cell is difficult to effectively simulate the transdermal diffusion process of oral mucosa during gargling, and the transdermal diffusion cell can be closer to the oral environment during gargling, so that the transdermal diffusion process of the oral mucosa during gargling can be simulated more accurately; further, a transdermal diffusion instrument capable of more accurately simulating the transdermal diffusion process of the oral mucosa in the gargling process is provided; in addition, an experimental method for oral mucosa transdermal diffusion is provided to simulate the oral mucosa transdermal diffusion process during mouth rinsing.
The technical scheme is as follows: the transdermal diffusion cell provided by the invention is used for oral mucosa transdermal diffusion experiments, and comprises a receiving cell and a dosing cell, wherein the dosing cell is buckled at the upper end of the receiving cell and is communicated with the receiving cell, and oral mucosa is arranged between the receiving dosing cell and the receiving cell, and the transdermal diffusion cell is characterized in that a stirring device is arranged at the upper end of the dosing cell and comprises:
the base is buckled at the upper end of the dosing pool;
the first power source is arranged on the base;
the first transmission unit is connected with the output end of the first power source;
the second transmission unit is arranged on the base, is connected with the first transmission unit, and comprises a driving gear and at least two driven gears which are arranged around the periphery of the driving gear and are meshed with the driving gear, and the driven gears are provided with screw rods;
the lifting platform is arranged on the screw rod through a screw rod nut, and the driven gear rotates to drive the screw rod to rotate so as to drive the lifting platform to move up and down;
the stirring unit comprises a second power source and a stirring rod, the second power source is arranged on the lifting platform, one end of the stirring rod is provided with a stirring paddle, the other end of the stirring rod is connected with the output end of the second power source, and the stirring rod penetrates through the base to enable the stirring paddle to be positioned in the drug feeding pool;
and the control module is connected with the first power source and the second power source.
Furthermore, the base is provided with a first through hole, a first rotating shaft is formed on the lower end face of the driving gear, a first bearing is sleeved on the first rotating shaft, and the first rotating shaft is arranged in the first through hole through the first bearing.
Furthermore, a second through hole is formed in the center of the driving gear, the first rotating shaft penetrates through the first rotating shaft, and the stirring rod penetrates through the driving gear from the first rotating shaft.
Furthermore, driven gear is 4, evenly sets up along the driving gear periphery.
Further, first drive unit is including setting up the action wheel at the output of first power supply, setting up from the driving wheel on the driving gear, and the action wheel passes through the transmission belt transmission with following the driving wheel, and it has the third through-hole to open from the driving wheel center, the stirring rake passes from the third through-hole.
Further, the stirring paddle rotates at a constant speed, the distance between the stirring paddle and the lower end face of the dosing pool is H cm, the upward or downward movement speed of the stirring paddle is V cm/s, and in terms of numerical value, V is kH, wherein k is 0.3-0.95.
Further, agitating unit still includes the protective housing of setting on the base, first transmission unit, second transmission unit all are located the protective housing inside.
Further, be provided with first sampling tube on the pond of dosing, first sampling tube one end and the pond intercommunication of dosing, the other end is opened has first sample connection, and being higher than of first sample connection is dosed the pond upper end.
Furthermore, a first cavity communicated with the first sampling tube is arranged on the first sampling tube.
Furthermore, a second sampling tube is arranged on the receiving pool, one end of the second sampling tube is communicated with the receiving pool, a second sampling port is formed in the other end of the second sampling tube, the second sampling port is higher than the upper end of the receiving pool, and a second containing cavity communicated with the second sampling tube is arranged on the second sampling tube.
Furthermore, a plurality of annular grooves are formed in the upper end face of the receiving pool, a plurality of annular bulges are correspondingly arranged on the lower end face of the dosing pool, and when the dosing pool is buckled on the receiving pool, the annular bulges are embedded into the corresponding annular grooves.
Further, a pressure detection sensor is arranged on the lower portion of the dosing pool and connected with the control module.
The transdermal diffusion instrument comprises the transdermal diffusion cell.
The transdermal diffusion experimental method comprises the following steps: determination of maximum impact pressure P that the oral mucosa can bearmaxAnd take 0.5Pmax～0.8PmaxAs impact force threshold P for the oral mucosa;
clamping oral mucosa on a diffusion pool, wherein the receiving pool is normal saline water, and the administration pool is mouthwash or toothpaste water;
placing the diffusion cell in a water bath box, heating to 37 ℃ in a water bath way, and preserving the temperature for 10 min;
setting the movement process of a stirring device, setting the stirring paddle to rotate at a constant speed, setting the distance between the stirring paddle and the lower end face of a dosing pool to be H cm, setting the speed of the stirring paddle to move up and down to be V cm/s, setting the stirring paddle to move at a uniform speed reduction when moving from top to bottom, setting the stirring paddle to move at a uniform acceleration when moving from bottom to top, setting the V to kH in a numerical value, wherein k is 0.3-0.95, and stopping the stirring paddle when detecting that the impact force at the oral mucosa exceeds P;
stirring for several seconds, and sampling and detecting.
Has the advantages that: compared with the prior art, the invention has the following advantages:
(1) compared with the prior art, the diffusion cell provided by the invention has the advantages that the stirring device, the base, the first power source, the first transmission unit, the second transmission unit, the lifting platform, the stirring unit and the control module are arranged at the upper end of the dosing cell, the second transmission unit comprises the driving gear and a plurality of at least two driven gears, and the driven gears are provided with the screw rods, so that the dosing cell can be stably stirred. And then can realize when carrying out oral mucosa and in the transdermal diffusion experiment, through stirring the solution in the pond of giving medicine for the solution environment in the pond of giving medicine is more close the environment in the oral cavity when gargling, makes the result of experiment oral mucosa transdermal diffusion experiment more accurate.
(2) The first sampling tube is arranged on the dosing pool, so that the solution in the dosing pool can be conveniently sampled and tested; and furthermore, the first sampling tube is provided with the first containing cavity, and the diffusion cell can be inclined when a proper sampling needle is lacked, so that sampling is facilitated.
(3) The receiving pool is provided with the second sampling tube, and the second sampling tube is provided with the second containing cavity communicated with the second sampling tube, so that the solution in the receiving pool can be conveniently sampled and tested.
(4) The upper end surface of the receiving pool is provided with the plurality of annular grooves, the lower end surface of the dosing pool is correspondingly provided with the plurality of annular bulges, and when the dosing pool is buckled on the receiving pool, the annular bulges are embedded into the corresponding annular grooves, so that the dosing pool and the receiving pool are convenient to locate mutually, the sealing is facilitated, and the solution is prevented from leaking outwards.
(5) The pressure detection sensor is arranged at the lower part of the administration pool, so that the impact force born by the oral mucosa can be monitored in real time, and the oral mucosa is prevented from being damaged by impact during a transdermal diffusion experiment.
(6) The transdermal diffusion instrument provided by the invention comprises the diffusion cell provided by the invention, and the transdermal diffusion instrument can be used for simulating the environment in the oral cavity during gargling, so that the transdermal diffusion experiment of the oral mucosa during gargling is effectively carried out, and the result has higher accuracy.
(7) By utilizing the transdermal diffusion experimental method, the environment in the diffusion cell is close to the environment in the oral cavity when gargling, so that the transdermal diffusion process of the oral mucosa can be effectively simulated, and compared with the existing method, the experimental result is more accurate.
Drawings
FIG. 1 is a structural view of a conventional transdermal diffusion cell;
FIG. 2 is a cross-sectional view of a transdermal diffusion cell of the present invention (without agitation);
FIG. 3 is a front view of a transdermal diffusion cell of the present invention;
FIG. 4 is an exploded view of a transdermal diffusion cell of the present invention;
FIG. 5 is a structural view of a stirring device of the present invention;
FIG. 6 is an exploded view of the stirring device of the present invention;
FIG. 7 is an exploded view of the stirring device of the present invention;
FIG. 8 is a cross-sectional view of a receiving tank of the present invention;
fig. 9 is a cross-sectional view of a dosing chamber of the present invention.
The figure shows that: 100. a receiving pool; 110. a second sampling tube; 111. a second sampling port; 120. a second cavity; 130. an annular groove;
200. a dosing pool; 210. a first sampling tube; 211. a first sampling port; 220. a first cavity; 230. an annular projection;
300. the mucosa of the oral cavity;
400. a magnetic stirrer;
500. a stirring device;
510. a base; 511. a first through hole; 512. mounting holes; 513. a projection;
520. a first power source;
530. a first transmission unit; 531. a driving wheel; 532. a transmission belt; 533. a driven wheel; 5331. a third through hole;
540. a second transmission unit; 541. a driving gear; 5411. a first rotating shaft; 5412. a first bearing; 5413. a second through hole; 542. a driven gear; 5421. a second rotating shaft; 5422. a second bearing; 543. a screw rod;
550. a lifting platform; 551. a feed screw nut;
560. a stirring unit; 561. a second power source; 562. a stirring rod; 563. a stirring paddle; 564. a coupling;
570. a protective shell;
600. and a pressure detection sensor.
Detailed Description
The present invention will be further described with reference to the following examples and figures in the specification, wherein the terms "first", "second", "third", etc. are used for convenience of description and are not intended to indicate or imply relative importance.
Referring to fig. 2, the transdermal diffusion cell of the present invention includes a receiving cell 100 and a dosing cell 200, the dosing cell 200 is fastened to the upper end of the receiving cell 100 and is communicated with the receiving cell 100, the oral mucosa 300 is disposed between the receiving and dosing cell 200 and the receiving cell 100, and a magnetic stirrer 400 is disposed in the receiving cell 100 during a transdermal test. Compared with the prior art, the difference of the invention is that as shown in fig. 3-4, the upper end of the administration pool 200 is provided with the stirring device 500, so that the solution in the administration pool 200 can be disturbed by stirring to generate a certain impact force on the oral mucosa 300, thereby being closer to the environment in the oral cavity during gargling and enabling the result obtained by the transdermal diffusion experiment to be more accurate. In order to make the environment in the dosing chamber 200 closer to the environment of the mouth when rinsing, as shown in fig. 5, 6 and 7, the stirring device 500 of the present invention comprises: the device comprises a base 510, a first power source 520, a first transmission unit 530, a second transmission unit 540, a lifting platform 550, a stirring unit 560 and a control module.
Wherein the base 510 is fastened to the upper end of the administration tank 200, and more particularly, in the embodiment of the present invention, the lower side of the base 510 is provided with the annular protrusion 513, and the annular protrusion 513 can be inserted into the administration tank 200, so that the stirring device 500 can be fixed to the administration tank 200, and when the solution in the administration tank 200 is stirred by the stirring device 500, the solution can be prevented from overflowing from the upper end of the administration tank 200.
The first power source 520 is disposed on the base 510, and in the embodiment of the present invention, the first power source 520 is a micro motor, because the size of the transdermal diffusion cell is relatively small, and the micro motor can be used as the power source to meet the size requirement.
The first transmission unit 530 is connected to an output end of the first power source 520 so as to transmit power output from the first power source 520.
The second transmission unit 540 is disposed on the base 510 and connected to the first transmission unit 530, the second transmission unit 540 includes a driving gear 541, at least two driven gears 542 disposed around the driving gear 541 and engaged with the driving gear 541, and a lead screw 543 is disposed on the driven gears 542. Specifically, in the embodiment of the present invention, a first through hole 511 is formed in the base 510, a first rotating shaft 5411 is formed on the lower end surface of the driving gear 541, a first bearing 5412 is sleeved on the first rotating shaft 5411, and the first rotating shaft 5411 is disposed in the first through hole 511 through the first bearing 5412, so that the driving gear can better rotate. Also, in the embodiment of the present invention, in order to mount the driven gear 542 and enable the driven gear 542 to rotate well, a mounting hole 512 for mounting the driven gear 542 may be provided on the base 510, a second rotation shaft 5421 may be formed on a lower end surface of the driven gear 542, a second bearing 5422 may be fitted over the second rotation shaft 5421, and the second rotation shaft 5421 may be disposed in the corresponding mounting hole 512 through the second bearing 5422.
The elevating platform 550 is provided on the lead screw 543 through the lead screw nut 551, and the driven gear 542 rotates to drive the lead screw 543 to rotate, thereby driving the elevating platform 550 to move up and down.
Specifically, the power output from the first power source 520 is transmitted to the second transmission unit 540 through the first transmission unit 530, and then the elevating platform 550 is driven to ascend or descend through the second transmission unit 540.
In the embodiment of the present invention, the first transmission unit 530 includes a driving gear 531 provided at an output end of the first power source 520, and a driven gear 533 provided at the driving gear 541, and the driving gear 531 and the driven gear 533 are transmitted by a transmission belt 532. When first power supply 520 during operation, drive action wheel 531 and rotate, then drive through drive belt 532 and follow driving wheel 533 and rotate, follow driving wheel 533 and drive driving gear 541 and rotate, driving gear 541 meshes with driven gear 542 to drive driven gear 542 and rotate, when driven gear 542 rotates, drive the lead screw 543 rotation of setting on driven gear 542, when lead screw 543 rotates, will rotate and convert the linear motion of screw-nut 551 into, thereby drive elevating platform 550 up-and-down motion.
In the embodiment of the present invention, at least two driven gears 542 and thus at least two screw rods 543 are provided, and all the driven gears 542 are driven by one driving gear 541, so that all the driven gears 542 rotate synchronously, and thus all the screw rods 543 still rotate synchronously, thereby ensuring the stability of the up-and-down movement process of the lifting platform 550 and further ensuring the stability of the stirring process.
For example, in one embodiment of the present invention, 4 driven gears 542 are uniformly arranged along the periphery of the driving gear 541, so that 4 lead screws 543 are provided to synchronously rotate to drive the lifting platform 550 to move up and down, thereby improving the stability of the lifting platform when the lifting platform is left to move, and further ensuring the stability when stirring.
The stirring unit 560 includes a second power source 561 and a stirring rod 562, wherein the second power source 561 is disposed on the lifting platform 550, similar to the first power source 520, and due to size limitation, the second power source 561 is also a micro motor in the embodiment of the present invention. One end of the stirring rod 562 is provided with a stirring paddle 563, the other end is connected with the output end of the second power source 561 through a coupler 564, and the stirring rod 562 penetrates through the base 510 to enable the stirring paddle 563 to be located in the dosing tank 200. Therefore, when elevating platform 550 up-and-down motion, can drive stirring unit 560 up-and-down motion to can adjust the position of stirring rake 563 in dosing pond 200, also can move from top to bottom when stirring rake 563 rotates simultaneously, make the environment in the oral cavity when rinsing one's mouth in dosing pond 200 more to the environment in the time of making transdermal diffusion experimental result have higher accuracy.
In order to reduce the volume of the stirring apparatus 500, so that the structure of the stirring apparatus 500 is more compact and has better structural stability, in the embodiment of the present invention, the center of the driving gear 541 is provided with a second through hole 5413, the second through hole 5413 penetrates through the first rotating shaft 5411, and the center of the driven wheel 533 of the first transmission unit 530 is provided with a third through hole 5331, the stirring rod 562 can penetrate through the driving gear 541 from the second through hole 5413 and penetrate through the driven wheel 533 from the third through hole 5331, i.e., the stirring rod 562, the driving gear 541 and the driven wheel 533 are in a coaxial state.
Further, in order to avoid entering dust and the like into each transmission unit, in an embodiment of the present invention, the stirring device 500 further includes a protective shell 570 disposed on the base 510, and the first transmission unit 530 and the second transmission unit 540 are both located inside the protective shell 570, so as to not only prevent dust and the like from entering, but also protect an operator and prevent the transmission unit from damaging the operator.
The control module is connected to the first and second power sources 520, 561 to enable control of the first and second power sources 520, 561. And for the control module, it can be set on the base 510, or it can be a separate external module, and in the experiment, it is connected with two power sources. The control module can include controller and touch-sensitive screen to can be according to the action of experiment demand control two power supplies.
Further, when the stirring device 500 is provided on the administration tank 200, there is a problem that it is inconvenient to sample the administration tank 200, and therefore, in order to solve the sampling problem, the administration tank 200 is provided with the first sampling pipe 210, one end of the first sampling pipe 210 communicates with the administration tank 200, and the other end is opened with the first sampling port 211, and specifically, as shown in fig. 2 and 9, the first sampling pipe 210 is inclined upward, and the first sampling port 211 is higher than the upper end surface of the administration tank 200. In current diffusion cell structure, the size of sampling tube is all longer relatively, this syringe needle that also leads to required sampler is longer, just can take a sample, consequently for the convenience of taking a sample, be provided with first appearance chamber 220 on first sampling tube 210, first appearance chamber 220 and first sampling tube 210 intercommunication, when the syringe needle at the sampler is short, can be when taking a sample, with the diffusion cell slope, make solution entering first appearance chamber 220 in the dosing cell 200, then shortened the sampling distance of sampler, thereby be convenient for take a sample.
For the same purpose, as shown in fig. 2 and 8, in the embodiment of the present invention, the receiving tank 100 is provided with a second sampling pipe 110, one end of the second sampling pipe 110 is communicated with the receiving tank 100, the other end is provided with a second sampling port 111, and the second sampling pipe 110 is inclined upwards, the second sampling port 111 is higher than the upper end of the receiving tank 100, so as to avoid the experiment that the solution overflows from the sampling port. In order to facilitate sampling, a second cavity 120 is disposed on the second sampling tube 110, and the second cavity 120 is communicated with the second sampling tube 110.
Further, for the current diffusion cell, the administration cell 200 is directly fastened on the receiving cell 100 and then clamped by a pipe clamp, but such a structure has many problems, on one hand, the administration cell 200 must be accurately fastened on the receiving cell 100 to ensure that the positions of the administration cell 200 and the receiving cell 100 are not greatly changed when clamped by the pipe clamp, and a high requirement is provided for the operation of experimenters; on the other hand, the solution is liable to leak out from the gap between the administration well 200 and the receiving well 100, and particularly, the solution is liable to leak out when the solution in the administration well 200 is disturbed. Therefore, in order to solve the above problems, in the embodiment of the present invention, as shown in fig. 8, a plurality of annular grooves 130 are formed on the upper end surface of the receiving well 100, as shown in fig. 9, a plurality of annular protrusions 230 are correspondingly formed on the lower end surface of the administration well 200, and when the administration well 200 is fastened to the receiving well 100, as shown in fig. 2, the annular protrusions 230 are inserted into the corresponding annular grooves 130. By arranging the annular protrusion 230 and the annular groove 130, on one hand, the annular protrusion 230 and the annular groove 130 are mutually positioned, so that the dosing tank 200 can be quickly buckled on the receiving tank 100 in an experiment, and the relative positions of the dosing tank 200 and the receiving tank 100 cannot be changed; on the other hand, after the pipe clamp is used for clamping, the solution is prevented from leaking due to the existence of the annular protrusion 230 and the annular groove 130, and particularly when the number of the annular protrusion 230 and the annular groove 130 exceeds 1, a labyrinth structure is formed, so that leakage is further reduced.
Further, in order to better simulate the environment in the oral cavity during mouth rinsing, in the embodiment of the present invention, the stirring paddle 563 may be implemented as follows, the stirring paddle 563 rotates at a constant speed, the distance from the stirring paddle 563 to the lower end surface of the administration tank 200 is H cm, the upward or downward movement speed of the stirring paddle 563 is V cm/s, and in terms of the value, V is kH, where k is 0.3 to 0.95, so that the stirring paddle 563 is implemented as uniform acceleration movement during upward movement and as uniform deceleration movement during downward movement.
Further, since the impact pressure that the oral mucosa 300 can bear during the in vitro experiment is limited, in order to avoid the excessive pressure that the oral mucosa 300 can bear due to the disturbance of the solution in the dosing tank 200 during the experiment and to avoid the breakage of the oral mucosa 300, in the embodiment of the present invention, the pressure detection sensor 600 is disposed at the lower portion of the dosing tank, and the detection pressure sensor 600 is connected to the control module. In the experiment, the maximum impact force P born by the oral mucosa can be tested in advancemaxThe impact force threshold for the oral mucosa is then determined based on this maximum impact force, e.g., in an embodiment of the invention, 0.5Pmax～0.8PmaxAs an impact force threshold. In the experiment, if the pressure detected by the pressure detection sensor 600 exceeds the impact force threshold, the pressure is fed back to the control module, and the control module controls the first power source 520 and the second power source 561 to act, so that the stirring paddle 563 decelerates or is far away from the oral mucosa 300, and the oral mucosa 300 is prevented from being damaged.
Based on the diffusion cell provided by the invention, the transdermal diffusion instrument is also provided, the experimental instrument comprises the diffusion cell and the water bath box, the transdermal diffusion process of the oral mucosa during mouth rinsing can be simulated by using the diffusion instrument, and compared with the existing experimental instrument, a more accurate experimental result can be obtained.
Further, the invention provides an oral mucosa transdermal diffusion experimental method, which comprises the following steps:
step 1: determination of maximum impact pressure P that the oral mucosa can bearmaxAnd take 0.5Pmax～0.8PmaxAs impact force threshold P for the oral mucosa;
step 2: clamping oral mucosa on a diffusion pool, wherein the receiving pool is normal saline water, and the administration pool is mouthwash or toothpaste water;
and step 3: placing the diffusion cell in a water bath box, heating to 37 ℃ in a water bath way, and preserving the temperature for 10 min;
and 4, step 4: setting the motion process of a stirring device, setting the stirring paddle to rotate at a constant speed, setting the distance between the stirring paddle and the lower end face of a dosing pool to be H cm, setting the up-and-down motion speed of the stirring paddle to be V cm/s, setting the stirring paddle to be uniform deceleration motion when moving from top to bottom, setting the stirring paddle to be uniform acceleration motion when moving from bottom to top, and setting the V to be kH in numerical value, wherein H is more than 0, and k is 0.3-0.95; for example, in one embodiment of the present invention, the paddle rotates at 60rpm, k is 0.3, and the paddle stops when the pressure detection sensor detects that the impact force at the oral mucosa exceeds P;
and 5: stirring for several seconds, and sampling and detecting.
By utilizing the experimental method, the environment in the diffusion pool is closer to the environment in the oral cavity when gargling, so that the transdermal diffusion process of the oral mucosa can be effectively simulated, and compared with the existing method, the experimental result is more accurate.
The above examples are only preferred embodiments of the present invention, it should be noted that: it will be apparent to those skilled in the art that various modifications and equivalents can be made without departing from the spirit of the invention, and it is intended that all such modifications and equivalents fall within the scope of the invention as defined in the claims.
Claims (14)
1. The utility model provides a transdermal diffusion cell for oral mucosa transdermal diffusion experiment, including receiving pond (100) and dosing pond (200), dosing pond (200) lock joint is in receiving pond (100) upper end and with receiving pond (100) intercommunication, and oral mucosa (300) set up and are receiving between dosing pond (200) and receiving pond (100), its characterized in that, dosing pond (200) upper end is provided with agitating unit (300), agitating unit (300) include:
the base (510) is buckled at the upper end of the dosing pool (200);
a first power source (520) disposed on the base (510);
a first transmission unit (530) connected to an output end of the first power source (520);
the second transmission unit (540) is arranged on the base (510), is connected with the first transmission unit (530), and comprises a driving gear (541) and at least two driven gears (542) which are arranged around the driving gear (541) and are meshed with the driving gear (541), wherein the driven gears (542) are provided with screw rods (543);
the lifting platform (550) is arranged on the screw rod (543) through a screw rod nut (551), and the driven gear (542) rotates to drive the screw rod (543) to rotate so as to drive the lifting platform (550) to move up and down;
the stirring unit (560) comprises a second power source (561) and a stirring rod (562), the second power source (561) is arranged on the lifting platform (550), one end of the stirring rod (562) is provided with a stirring paddle (563), the other end of the stirring rod is connected with the output end of the second power source (561), and the stirring rod (562) penetrates through the base (510) to enable the stirring paddle (563) to be located in the dosing pool (200);
a control module connected with the first power source (520) and the second power source (561).
2. The transdermal diffusion cell according to claim 1, wherein the base (510) is provided with a first through hole (511), the driving gear (541) is provided with a first rotating shaft (5411) on a lower end surface thereof, the first rotating shaft (5411) is sleeved with a first bearing (5412), and the first rotating shaft (5411) is disposed in the first through hole (511) through the first bearing (5412).
3. The transdermal diffusion cell of claim 2, wherein the driving gear (541) has a second through hole (5413) formed at the center thereof, the first shaft (5412) penetrates the first shaft (5411), and the stirring rod (562) penetrates the driving gear (541) from the first shaft (5413).
4. The transdermal diffusion cell according to claim 3, wherein the number of the driven gears (542) is 4, and the driven gears are uniformly arranged along the periphery of the driving gear (541).
5. The transdermal diffusion cell according to claim 1, wherein the first transmission unit (530) comprises a driving wheel (531) disposed at the output end of the first power source (520), and a driven wheel (533) disposed on the driving wheel (541), the driving wheel (531) and the driven wheel (533) are driven by a transmission belt (532), a third through hole (5331) is formed in the center of the driven wheel (533), and the paddle (563) passes through the third through hole (5331).
6. The transdermal diffusion cell according to claim 1, wherein the stirring paddle (563) rotates at a constant speed, the distance from the stirring paddle (563) to the lower end face of the administration cell (200) is H cm, the upward or downward speed of the stirring paddle (563) is V cm/s, and in terms of the value, V ═ kH, wherein k is 0.3 to 0.95.
7. The transdermal diffusion cell according to any one of claims 1 to 6, wherein the stirring device (300) further comprises a protective housing (570) disposed on the base (510), and the first transmission unit (530) and the second transmission unit (540) are both located inside the protective housing (570).
8. The transdermal diffusion cell according to claim 7, wherein a first sampling tube (210) is disposed on the administration cell (200), one end of the first sampling tube (210) is communicated with the administration cell (200), and the other end is provided with a first sampling port (211), and the first sampling port (211) is higher than the upper end of the administration cell (200).
9. The transdermal diffusion cell according to claim 8, wherein the first sampling tube (210) is provided with a first cavity (220) communicated with the first sampling tube (210).
10. The transdermal diffusion cell according to claim 8, wherein a second sampling tube (110) is arranged on the receiving cell (100), one end of the second sampling tube (110) is communicated with the receiving cell (100), the other end of the second sampling tube is provided with a second sampling port (111), the second sampling port (111) is higher than the upper end of the receiving cell (100), and a second cavity (120) communicated with the second sampling tube (110) is arranged on the second sampling tube (110).
11. The transdermal diffusion cell according to claim 10, characterized in that the receiving cell (100) is provided with a plurality of annular grooves (130) at the upper end surface, and the administration cell (200) is correspondingly provided with a plurality of annular protrusions (230) at the lower end surface, and when the administration cell (200) is buckled on the receiving cell (100), the annular protrusions (130) are embedded in the corresponding annular grooves (230).
12. The transdermal diffusion cell according to claim 1, wherein a pressure detection sensor (600) is arranged at the lower part of the administration cell (200), and the pressure detection sensor (600) is connected with a control module.
13. A transdermal diffusion device comprising the transdermal diffusion cell according to any one of claims 1 to 12.
14. A transdermal diffusion assay method, comprising: determination of maximum impact pressure P that the oral mucosa can bearmaxAnd take 0.5Pmax～0.8PmaxAs impact force threshold P for the oral mucosa;
clamping oral mucosa on a diffusion pool, wherein the receiving pool is normal saline water, and the administration pool is mouthwash or toothpaste water;
placing the diffusion cell in a water bath box, heating to 37 ℃ in a water bath manner, and preserving heat for 10 min;
setting the movement process of a stirring device, setting the stirring paddle to rotate at a constant speed, setting the distance between the stirring paddle and the lower end face of a dosing pool to be H cm, setting the speed of the stirring paddle to move up and down to be V cm/s, setting the stirring paddle to move at a uniform speed reduction when moving from top to bottom, setting the stirring paddle to move at a uniform acceleration when moving from bottom to top, setting the V to kH in a numerical value, wherein k is 0.3-0.95, and stopping the stirring paddle when detecting that the impact force at the oral mucosa exceeds P;
stirring for several seconds, and sampling and detecting.
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