CN113029958B - Dynamic light scattering detection device for detecting DNA denaturation - Google Patents
Dynamic light scattering detection device for detecting DNA denaturation Download PDFInfo
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- CN113029958B CN113029958B CN202110357238.XA CN202110357238A CN113029958B CN 113029958 B CN113029958 B CN 113029958B CN 202110357238 A CN202110357238 A CN 202110357238A CN 113029958 B CN113029958 B CN 113029958B
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- 238000002296 dynamic light scattering Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 238000004925 denaturation Methods 0.000 title claims abstract description 14
- 230000036425 denaturation Effects 0.000 title claims abstract description 14
- 238000005192 partition Methods 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 3
- 230000009471 action Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000002776 aggregation Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000005054 agglomeration Methods 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012576 optical tweezer Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
- G01N2015/0238—Single particle scatter
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to the field of dynamic light scattering detection devices, in particular to a dynamic light scattering detection device for detecting DNA denaturation, which aims at the problems that the existing dynamic light scattering detection device is imperfect in clamping and fixing of a Markov Zeta potential sample cell, and further the sample cell is inclined or swayed in the experimental process possibly to cause errors in experimental results, and a laser is easy to incline after being used for a period of time.
Description
Technical Field
The invention relates to the technical field of dynamic light scattering detection devices, in particular to a dynamic light scattering detection device for detecting DNA denaturation.
Background
De-agglomeration of DNA facilitates transcription, whereas agglomeration of DNA facilitates replication. Thus, understanding how DNA aggregates and how it deagglomerates is an important part of understanding life progress. The DNA condensation process plays an important role in the biological therapeutic process. In biotechnology or medicine, research on DNA aggregation provides a promising approach to the preparation of DNA containing a gene of therapeutic interest, facilitating transfer from solution to target cells for gene therapy. The nanoscale success of DNA in the life system is very important for detection and treatment in various situations. The current methods for DNA aggregation research mainly comprise dynamic light scattering, atomic force microscopy, and recently developed single-molecule technologies such as optical tweezers and magnetic tweezers.
Dynamic light scattering is mainly used for detecting the change of scattered light intensity, frequency and time, and when light is directly irradiated to a surface to be detected of a sample, scattered light is generated. The charge and size of the substance can be measured by detecting the frequency and intensity of the scattered light generated with time, so that the DLS experiment can measure not only the particle size, i.e. the particle diameter, but also the Zeta potential, i.e. the electrophoretic mobility, of the substance. The measuring method has the advantages of high efficiency, good repeatability, high accuracy and the like during measurement.
However, the existing dynamic light scattering detection device is not perfect in terms of clamping and fixing the markov Zeta potential sample cell, so that the sample cell may incline or shake during the experimental process, resulting in error of the experimental result, and the laser is easy to incline after being used for a period of time, so we propose a dynamic light scattering detection device for detecting DNA denaturation, so as to solve the above-mentioned problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a dynamic light scattering detection device for detecting DNA denaturation.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a developments light scattering detection device for detecting DNA denaturation, includes operation panel and Markov Zeta potential sample cell, the top of operation panel is provided with attenuator, observation box, first detector, digital signal processor, display and second detector, attenuator and observation box all are in same straight line with the laser instrument setting, and the attenuator is located between laser instrument and the observation box, first detector and second detector all with digital signal processor electric connection, digital signal processor and display electric connection, the below of laser instrument is provided with the regulation subassembly that is used for adjusting the laser instrument horizontally, markov Zeta potential sample cell is placed in the inside of observation box, the mounting groove has all been seted up to the both sides inner wall of observation box, the inside of mounting groove is provided with the clamping assembly who is used for pressing from both sides tight Markov Zeta potential sample cell, the top of observation box is provided with two fixed subassemblies that are used for fixing the Markov Zeta potential sample cell of symmetry setting, the bottom four corners of operation panel all fixedly connected with support column.
Preferably, the adjusting component comprises a supporting rod fixedly connected to the top of the operating platform, the top of the supporting rod is rotationally connected with the bottom of the laser, the bottom of the laser is rotationally connected with a threaded block, the internal threads of the threaded block are connected with a screw rod, and the bottom of the screw rod is rotationally penetrated through the operating platform and is fixedly connected with a first rotating plate.
Preferably, the clamping assembly comprises a rotating shaft which is connected to the inner walls of two sides of the mounting groove, a second rotating plate is fixedly sleeved on the outer wall of the rotating shaft, an L-shaped plate is fixedly connected to the top of the second rotating plate, a tension spring is fixedly connected to one side of the L-shaped plate, which is far away from the Markov Zeta potential sample pool, the other end of the tension spring is fixedly connected with the inner wall of one side of the mounting groove, and a clamping groove is formed in the top of the L-shaped plate.
Preferably, the fixed subassembly includes fixed plate at the observation box top of fixed plate's inside slip through connection has the pull rod, the one end fixedly connected with arm-tie of Markov Zeta potential sample cell is kept away from to the pull rod, the other end fixedly connected with baffle of pull rod, fixedly connected with same first spring between fixed plate and the baffle, first spring housing is established on the pull rod, triangular groove has been seted up to the bottom of baffle.
Preferably, the top inner wall fixedly connected with symmetry of mounting groove sets up two second springs, the bottom fixedly connected with backup pad of second spring, the backup pad is close to the one end fixedly connected with lug of markov Zeta potential sample cell, the top of lug slides and runs through the top of observing the box and fixedly connected with triangle piece, the bottom and the draw-in groove looks block of lug, triangle piece and triangle groove looks block.
Preferably, the top fixedly connected with baffle of operation panel, the through-hole has been seted up to the inside of baffle, the baffle sets up between laser instrument and attenuator, the baffle is close to one side of laser instrument and is provided with a plurality of light sensors.
Preferably, the second detector is located at one side of the observation box and forms an included angle of 90 degrees with the observation box, and the first detector is located at one side of the observation box and forms an included angle of 173 degrees with the observation box.
Preferably, one end of the L-shaped plate, which is close to the Markov Zeta potential sample cell, is fixedly connected with a rubber block.
Compared with the prior art, the invention has the beneficial effects that:
1. placing the Markov Zeta potential sample cell in an observation box, pushing the second rotating plate to rotate by the Markov Zeta potential sample cell, driving the L-shaped plate to rotate by the second rotating plate to drive the rubber block to rotate and stretch the tension spring, wherein the rubber block is in contact with two sides of the Markov Zeta potential sample cell at the moment, and further clamping the Markov Zeta potential sample cell;
2. the supporting plate vertically moves downwards under the action of the elastic force of the second spring, the supporting plate drives the protruding block to vertically move downwards and then to be clamped with the clamping groove, the protruding block drives the triangular block to vertically move downwards, at the moment, the baffle transversely moves under the action of the elastic force of the first spring, the bottom of the baffle is abutted against the top of the Markov Zeta potential sample cell, and the function of fixing the Markov Zeta potential sample cell is achieved;
3. when the Markov Zeta potential sample pool needs to be taken out, the pull plate is transversely pulled by hands, the pull plate drives the pull rod to transversely move, the pull rod drives the baffle to transversely move and extrude the first spring, the L-shaped plate rotates under the action of the tension force of the tension spring, the L-shaped plate drives the second rotating plate to rotate, the L-shaped plate pushes the convex block to vertically move upwards, at the moment, the bottom of the convex block is in contact with the top of the L-shaped plate, and the rotation of the second rotating plate can drive the Markov Zeta potential sample pool to vertically move upwards, so that the Markov Zeta potential sample pool is conveniently taken out;
4. when the light sensor detects light, the laser is further indicated to be inclined, the first rotating plate is rotated, the first rotating plate drives the screw rod to rotate, the screw rod drives the threaded block to vertically move upwards, and the height of the laser, which is close to one end of the partition plate, is adjusted until the light sensor cannot detect the light, and the light is horizontally emitted from the partition plate.
According to the invention, the Markov Zeta potential sample cell is conveniently clamped through the arrangement of the clamping component, the fixing of the Markov Zeta potential sample cell is ensured through the fixing component, the condition that the sample cell is inclined or swayed is avoided, further, the experiment error is avoided, the Markov Zeta potential sample cell can be taken out by transversely pulling the pull plate, and the arrangement of the partition plate and the light sensor ensures that the laser is kept in a horizontal state during the experiment.
Drawings
FIG. 1 is a schematic diagram of a dynamic light scattering detection device for detecting DNA denaturation according to the present invention;
FIG. 2 is a schematic diagram showing the front view of a dynamic light scattering detector for detecting DNA denaturation according to the present invention;
FIG. 3 is a schematic view of a cross-sectional front view of the observation box of the present invention;
FIG. 4 is an enlarged view of section A of the present invention;
FIG. 5 is a schematic diagram of a front view of the observation box according to the present invention;
FIG. 6 is a connection diagram of a tie rod, a tie plate and a baffle plate according to the present invention;
fig. 7 is a schematic view of a cross-sectional front view of a separator according to the present invention.
In the figure: 1. a laser; 2. an attenuator; 3. an observation box; 4. a first detector; 5. a digital signal processor; 6. a display; 7. a second detector; 8. a screw block; 9. a through hole; 10. a partition plate; 11. a first rotating plate; 12. a screw; 13. an operation table; 14. a support rod; 15. a support column; 16. a markov Zeta potential sample cell; 17. a bump; 18. a baffle; 19. triangular grooves; 20. triangular blocks; 21. a first spring; 22. a fixing plate; 23. pulling a plate; 24. a pull rod; 25. a second spring; 26. a support plate; 27. a tension spring; 28. a clamping groove; 29. a mounting groove; 30. a rotating shaft; 31. an L-shaped plate; 32. a second rotating plate; 33. a rubber block; 34. and a light sensor.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
Example 1
Referring to fig. 1-7, a dynamic light scattering detection device for detecting DNA denaturation comprises an operation table 13 and a malvern Zeta potential sample cell 16, wherein an attenuator 2, an observation box 3, a first detector 4, a digital signal processor 5, a display 6 and a second detector 7 are arranged at the top of the operation table 13, the attenuator 2 and the observation box 3 are all arranged on the same straight line with a laser 1, the attenuator 2 is positioned between the laser 1 and the observation box 3, the first detector 4 and the second detector 7 are all electrically connected with the digital signal processor 5, the digital signal processor 5 is electrically connected with the display 6, an adjusting component for adjusting the level of the laser 1 is arranged below the laser 1, the malvern Zeta potential sample cell 16 is placed in the observation box 3, mounting grooves 29 are formed in the inner walls of the two sides of the observation box 3, two symmetrically arranged fixing components for fixing the malvern Zeta potential sample cell 16 are arranged in the inner walls of the mounting grooves 29, and support columns 15 are fixedly connected at the bottom of the operation table 13.
Example two
The present embodiment is modified based on the first embodiment: the adjusting component comprises a supporting rod 14 fixedly connected to the top of an operating platform 13, the top of the supporting rod 14 is rotationally connected with the bottom of the laser 1, the bottom of the laser 1 is rotationally connected with a threaded block 8, the internal threaded connection of the threaded block 8 is provided with a threaded rod 12, the bottom of the threaded rod 12 is rotationally connected with the operating platform 13 and fixedly connected with a first rotating plate 11, the clamping component comprises a same rotating shaft 30 rotationally connected to the inner walls of two sides of a mounting groove 29, a second rotating plate 32 is fixedly sleeved on the outer wall of the rotating shaft 30, an L-shaped plate 31 is fixedly connected to the top of the second rotating plate 32, a tension spring 27 is fixedly connected to one side of the L-shaped plate 31, which is far away from the Markov Zeta potential sample cell 16, the other end of the tension spring 27 is fixedly connected with the inner wall of one side of the mounting groove 29, a clamping groove 28 is formed in the top of the L-shaped plate 31, the fixing component comprises a fixing plate 22 fixedly connected to the top of the observation box 3, a pull rod 24 is fixedly connected to the inside of the fixing plate 22, one end of the pull rod 24 is fixedly connected with a pull plate 23, the other end of the pull rod 24 is fixedly connected with a baffle 18, the same first spring 21 is fixedly connected between the fixing plate 22 and the baffle 18, and the baffle 18 is fixedly connected to the first spring 21 and the first spring 21 is sleeved on the bottom of the third spring 19.
Example III
The present embodiment is modified based on the first embodiment: the top inner wall fixedly connected with of mounting groove 29 two second springs 25 that the symmetry set up, the bottom fixedly connected with backup pad 26 of second spring 25, the backup pad 26 is close to the one end fixedly connected with lug 17 of markov Zeta potential sample cell 16, the top of lug 17 slides and runs through the top of observing box 3 and fixedly connected with triangle 20, the bottom of lug 17 and draw-in groove 28 looks block, triangle 20 and triangle 19 looks block, the top fixedly connected with baffle 10 of operation panel 13, through-hole 9 has been seted up to the inside of baffle 10, baffle 10 sets up between laser 1 and attenuator 2, the baffle 10 is close to one side of laser 1 is provided with a plurality of light sensor 34, second detector 7 is located one side of observing box 3 and is 90 degrees contained angles with observing box 3, first detector 4 is located one side of observing box 3 and is 173 degrees contained angles with observing box 3, the one end fixedly connected with rubber piece 33 that L template 31 is close to markov Zeta potential sample cell 16.
Working principle: when in use, the Markov Zeta potential sample cell 16 is placed in the observation box 3, the Markov Zeta potential sample cell 16 pushes the second rotating plate 32 to rotate, the second rotating plate 32 drives the L-shaped plate 31 to rotate, the L-shaped plate 31 drives the rubber block 33 to rotate and stretch the tension spring 27, the rubber block 33 is in contact with two sides of the Markov Zeta potential sample cell 16 at the moment, further clamps the Markov Zeta potential sample cell 16, the supporting plate 26 vertically moves downwards under the action of the elasticity of the second spring 25, the supporting plate 26 drives the protruding block 17 vertically moves downwards and is further clamped with the clamping groove 28, the protruding block 17 drives the triangular block 20 vertically moves downwards, at the moment, the baffle 18 transversely moves under the action of the elasticity of the first spring 21, further the bottom of the baffle 18 is in contact with the top of the Markov Zeta potential sample cell 16, further the function of fixing the Markov Zeta potential sample cell 16 is achieved, when the Markov Zeta potential sample cell 16 needs to be taken out, pulling the pull plate 23 transversely by hand, the pull plate 23 drives the pull rod 24 to move transversely, the pull rod 24 drives the baffle 18 to move transversely and extrude the first spring 21, the L-shaped plate 31 rotates under the pulling force of the tension spring 27, the L-shaped plate 31 drives the second rotating plate 32 to rotate, the L-shaped plate 31 pushes the lug 17 to move vertically upwards, the bottom of the lug 17 is in contact with the top of the L-shaped plate 31, the rotation of the second rotating plate 32 can drive the Markov Zeta potential sample cell 16 to move vertically upwards, the Markov Zeta potential sample cell 16 is convenient to take out, when the light sensor 34 detects light, the laser 1 is further indicated to be inclined, the first rotating plate 11 rotates, the first rotating plate 11 drives the screw 12 to rotate, the screw 12 drives the threaded block 8 to move vertically upwards, the height of the laser 1 close to one end of the partition plate 10 is further adjusted until the light sensor 34 can not detect the light, indicating that light is emitted horizontally from within the baffle 10.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (4)
1. The utility model provides a dynamic light scattering detection device for detecting DNA denaturation, includes operation panel (13) and marketspace potential sample cell (16), its characterized in that, the top of operation panel (13) is provided with attenuator (2), observation box (3), first detector (4), digital signal processor (5), display (6) and second detector (7), attenuator (2) and observation box (3) all set up on same straight line with laser instrument (1), attenuator (2) are located between laser instrument (1) and observation box (3), first detector (4) and second detector (7) all with digital signal processor (5) electric connection, digital signal processor (5) and display (6) electric connection, the below of laser instrument (1) is provided with the adjusting part that is used for adjusting laser instrument (1) horizontally, marketspace potential sample cell (16) are placed in the inside of observation box (3), both sides inner wall of observation box (3) all sets up between laser instrument (1) and observation box (3), install the two clamping potential sample cell (16) of installing (29) and are used for setting up the fixed sample cell of two clamping potential (16) of the setting up, the clamping potential sample cell (16), support columns (15) are fixedly connected to four corners of the bottom of the operation table (13);
the adjusting component comprises a supporting rod (14) fixedly connected to the top of the operating platform (13), the top of the supporting rod (14) is rotationally connected with the bottom of the laser (1), the bottom of the laser (1) is rotationally connected with a threaded block (8), the internal threads of the threaded block (8) are connected with a screw rod (12), and the bottom of the screw rod (12) rotationally penetrates through the operating platform (13) and is fixedly connected with a first rotating plate (11);
the clamping assembly comprises a same rotating shaft (30) which is rotationally connected to the inner walls of the two sides of the mounting groove (29), a second rotating plate (32) is fixedly sleeved on the outer wall of the rotating shaft (30), an L-shaped plate (31) is fixedly connected to the top of the second rotating plate (32), a tension spring (27) is fixedly connected to one side, far away from the Markov Zeta potential sample pool (16), of the L-shaped plate (31), the other end of the tension spring (27) is fixedly connected with the inner wall of one side of the mounting groove (29), and a clamping groove (28) is formed in the top of the L-shaped plate (31);
the fixed assembly comprises a fixed plate (22) fixedly connected to the top of the observation box (3), a pull rod (24) is connected in a sliding penetrating manner in the fixed plate (22), one end of the pull rod (24) away from the Markov Zeta potential sample pool (16) is fixedly connected with a pull plate (23), the other end of the pull rod (24) is fixedly connected with a baffle (18), the fixed plate (22) and the baffle (18) are fixedly connected with the same first spring (21), the first spring (21) is sleeved on the pull rod (24), and a triangular groove (19) is formed in the bottom of the baffle (18);
two second springs (25) that the top inner wall fixedly connected with symmetry of mounting groove (29) set up, the bottom fixedly connected with backup pad (26) of second spring (25), one end fixedly connected with lug (17) that backup pad (26) are close to markov Zeta potential sample cell (16), the top of lug (17) slides and runs through the top of observing box (3) and fixedly connected with triangle piece (20), the bottom and draw-in groove (28) looks block of lug (17), triangle piece (20) and triangle groove (19) looks block.
2. The dynamic light scattering detection device for detecting DNA denaturation according to claim 1, wherein a partition board (10) is fixedly connected to the top of the operation table (13), a through hole (9) is formed in the partition board (10), the partition board (10) is arranged between the laser (1) and the attenuator (2), and a plurality of light sensors (34) are arranged on one side, close to the laser (1), of the partition board (10).
3. A dynamic light scattering detection device for detecting DNA denaturation according to claim 1, wherein the second detector (7) is located at one side of the observation box (3) and forms an angle of 90 degrees with the observation box (3), and the first detector (4) is located at one side of the observation box (3) and forms an angle of 173 degrees with the observation box (3).
4. The dynamic light scattering detecting device for detecting DNA denaturation according to claim 1, wherein a rubber block (33) is fixedly connected to one end of the L-shaped plate (31) close to the Markov Zeta potential sample cell (16).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110357238.XA CN113029958B (en) | 2021-04-01 | 2021-04-01 | Dynamic light scattering detection device for detecting DNA denaturation |
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| CN202110357238.XA CN113029958B (en) | 2021-04-01 | 2021-04-01 | Dynamic light scattering detection device for detecting DNA denaturation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8705040B2 (en) * | 2004-03-06 | 2014-04-22 | Michael Trainer | Methods and apparatus for determining particle characteristics by measuring scattered light |
| US8697129B2 (en) * | 2011-03-02 | 2014-04-15 | Imra America, Inc. | Stable colloidal gold nanoparticles with controllable surface modification and functionalization |
| EP2742337B1 (en) * | 2011-08-19 | 2016-05-04 | Malvern Instruments Ltd | Dual-mode characterization of particulates |
| CN103776802B (en) * | 2014-01-10 | 2016-06-08 | 上海理工大学 | The miniflow measuring viscoelastic fluid becomes measurement apparatus and method |
| US10119910B2 (en) * | 2015-10-09 | 2018-11-06 | Malvern Panalytical Limited | Particle characterisation instrument |
| US10365198B2 (en) * | 2016-04-21 | 2019-07-30 | Malvern Panalytical Limited | Particle characterization |
| DE202016006846U1 (en) * | 2016-11-07 | 2016-12-23 | Particle Metrix Gmbh | Device for measuring the concentration and size of nanoparticles in liquids in scattered light mode and in fluorescence mode |
| CN110987738A (en) * | 2019-12-26 | 2020-04-10 | 上海绿帝环保科技有限公司 | Automatic turbid liquid detection device and method |
| CN112067511A (en) * | 2020-10-10 | 2020-12-11 | 丹东百特仪器有限公司 | Nano-particle analyzer based on back scattering cross-correlation technology and detection method thereof |
| CN112504922B (en) * | 2020-10-20 | 2022-09-02 | 华南师范大学 | Online measurement system and method for particle size distribution of atmospheric particulates |
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