CN113029958B - Dynamic light scattering detection device for detecting DNA denaturation - Google Patents
Dynamic light scattering detection device for detecting DNA denaturation Download PDFInfo
- Publication number
- CN113029958B CN113029958B CN202110357238.XA CN202110357238A CN113029958B CN 113029958 B CN113029958 B CN 113029958B CN 202110357238 A CN202110357238 A CN 202110357238A CN 113029958 B CN113029958 B CN 113029958B
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- sample cell
- observation box
- zeta potential
- potential sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000002296 dynamic light scattering Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 238000004925 denaturation Methods 0.000 title claims abstract description 13
- 230000036425 denaturation Effects 0.000 title claims abstract description 13
- 238000005192 partition Methods 0.000 claims abstract description 16
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 7
- 238000009434 installation Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 230000005494 condensation Effects 0.000 description 4
- 238000009833 condensation Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012576 optical tweezer Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
- G01N2015/0238—Single particle scatter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明属于动态光散射检测装置领域，尤其是一种用于检测DNA变性的动态光散射检测装置，针对现有的动态光散射检测装置对于马尔文Zeta电位样品池的夹紧和固定方面做得并不完善，进而在实验过程中可能出现样品池倾斜或晃动的情况，导致实验结果产生误差，并且激光器在使用一段时间后，容易出现倾斜的情况的问题，现提出如下方案，其包括操作台和马尔文Zeta电位样品池，本发明中，通过夹紧组件的设置便于夹紧马尔文Zeta电位样品池，并且通过固定组件保证马尔文Zeta电位样品池的固定，避免样品池倾斜或晃动的情况，进而避免实验产生误差，横向拉动拉板即可取出马尔文Zeta电位样品池，隔板和光线传感器的设置保证激光器在实验时保持水平状态。
The present invention belongs to the field of dynamic light scattering detection devices, especially a dynamic light scattering detection device for detecting DNA denaturation. In view of the imperfect clamping and fixing of the Malvern Zeta potential sample cell in the existing dynamic light scattering detection device, the sample cell may tilt or shake during the experiment process, resulting in errors in the experimental results, and the problem that the laser is prone to tilt after a period of use. The Malvern Zeta potential sample cell, and the fixed component ensures the fixation of the Malvern Zeta potential sample cell to avoid the sample cell tilting or shaking, thereby avoiding errors in the experiment, and the Malvern Zeta potential sample cell can be taken out by pulling the pull plate laterally. The settings of the partition and the light sensor ensure that the laser remains in a horizontal state during the experiment.
Description
技术领域technical field
本发明涉及动态光散射检测装置技术领域，尤其涉及一种用于检测DNA变性的动态光散射检测装置。The invention relates to the technical field of dynamic light scattering detection devices, in particular to a dynamic light scattering detection device for detecting DNA denaturation.
背景技术Background technique
DNA的解凝聚有利于转录的完成，而DNA的凝聚则有利于复制的完成。因此，理解探究DNA如何凝聚及其如何解凝聚是理解生命进程的一个重要部分。DNA凝聚过程在生物治疗过程扮演重要角色。在生物技术或医学中，DNA凝聚的研究提供了一种很有前途的方法来对含有治疗目的基因的DNA进行制备，有利于从溶液转移到靶细胞以进行基因治疗。DNA在生命系统中的纳米级成功对于各种情况下的检测和治疗是非常重要的。目前用于DNA凝聚研究的方法主要有动态光散射、原子力显微镜和最新开发的光镊和磁镊等的单分子技术。DNA decondensation facilitates the completion of transcription, while DNA condensation facilitates the completion of replication. Understanding how DNA condenses and how it uncondenses is therefore an important part of understanding the processes of life. The DNA condensation process plays an important role in the biotherapeutic process. In biotechnology or medicine, the study of DNA condensation provides a promising method to prepare DNA containing genes of therapeutic interest, which facilitates transfer from solution to target cells for gene therapy. The nanoscale success of DNA in living systems is of great importance for the detection and treatment of various conditions. At present, the methods used in the study of DNA condensation mainly include dynamic light scattering, atomic force microscopy, and newly developed single-molecule techniques such as optical tweezers and magnetic tweezers.
动态光散射主要用于检测散射光强度、频率与时间的变化，当光直射到样品待测面时，产生散射光。通过检测产生的散射光的频率随时间的变化以及其强度就可测量物质的带电量与尺寸大小，因此，DLS实验不仅可以测出粒子的大小，即粒径，还可以测量物质的Zeta电位，即电泳迁移率。该测量方法测量时具有效率高、可重复性好、准确度高等优点。Dynamic light scattering is mainly used to detect changes in the intensity, frequency and time of scattered light. When the light directly hits the surface of the sample to be tested, scattered light is generated. The charge and size of the substance can be measured by detecting the frequency of the scattered light and its intensity over time. Therefore, the DLS experiment can not only measure the size of the particle, that is, the particle diameter, but also measure the Zeta potential of the substance, that is, the electrophoretic mobility. The measurement method has the advantages of high efficiency, good repeatability, high accuracy and the like during measurement.
但是现有的动态光散射检测装置对于马尔文Zeta电位样品池的夹紧和固定方面做得并不完善，进而在实验过程中可能出现样品池倾斜或晃动的情况，导致实验结果产生误差，并且激光器在使用一段时间后，容易出现倾斜的情况，所以我们提出一种用于检测DNA变性的动态光散射检测装置，用以解决上述所提到的问题。However, the existing dynamic light scattering detection device is not perfect for the clamping and fixing of the Malvern Zeta potential sample cell, and the sample cell may be tilted or shaken during the experiment, resulting in errors in the experimental results, and the laser is prone to tilt after a period of use, so we propose a dynamic light scattering detection device for detecting DNA denaturation to solve the above-mentioned problems.
发明内容Contents of the invention
本发明的目的是为了解决现有技术中存在的缺点，而提出的一种用于检测DNA变性的动态光散射检测装置。The object of the present invention is to solve the shortcomings in the prior art, and propose a dynamic light scattering detection device for detecting DNA denaturation.
为了实现上述目的，本发明采用了如下技术方案：In order to achieve the above object, the present invention adopts the following technical solutions:
一种用于检测DNA变性的动态光散射检测装置，包括操作台和马尔文Zeta电位样品池，所述操作台的顶部设置有衰减器、观测盒、第一检测器、数字信号处理器、显示器和第二检测器，所述衰减器和观测盒均与激光器设置在同一直线上，衰减器位于激光器和观测盒之间，所述第一检测器和第二检测器均与数字信号处理器电性连接，所述数字信号处理器与显示器电性连接，所述激光器的下方设置有用于调节激光器水平的调节组件，所述马尔文Zeta电位样品池放置在观测盒的内部，所述观测盒的两侧内壁均开设有安装槽，所述安装槽的内部设置有用于夹紧马尔文Zeta电位样品池的夹紧组件，所述观测盒的顶部设置有对称设置的两个用于固定马尔文Zeta电位样品池的固定组件，所述操作台的底部四角均固定连接有支撑柱。A dynamic light scattering detection device for detecting DNA denaturation, comprising an operating table and a Malvern Zeta potential sample cell, the top of the operating table is provided with an attenuator, an observation box, a first detector, a digital signal processor, a display and a second detector, the attenuator and the observation box are arranged on the same line as the laser, the attenuator is located between the laser and the observation box, the first detector and the second detector are electrically connected to a digital signal processor, the digital signal processor is electrically connected to a display, and an adjustment component for adjusting the level of the laser is arranged below the laser , the Malvern Zeta potential sample cell is placed inside the observation box, the inner walls of both sides of the observation box are provided with installation grooves, the inside of the installation groove is provided with a clamping assembly for clamping the Malvern Zeta potential sample cell, the top of the observation box is provided with two symmetrically arranged fixed assemblies for fixing the Malvern Zeta potential sample cell, and the four corners of the bottom of the console are fixedly connected with support columns.
优选地，所述调节组件包括固定连接在操作台顶部的支撑杆，所述支撑杆的顶部与激光器的底部转动连接，所述激光器的底部转动连接有螺纹块，所述螺纹块的内部螺纹连接有螺杆，所述螺杆的底部转动贯穿操作台并固定连接有第一转板。Preferably, the adjustment assembly includes a support rod fixedly connected to the top of the operating table, the top of the supporting rod is rotatably connected to the bottom of the laser, the bottom of the laser is rotatably connected to a threaded block, the inner thread of the threaded block is connected to a screw, and the bottom of the screw rotates through the operating table and is fixedly connected to the first rotating plate.
优选地，所述夹紧组件包括转动连接在安装槽两侧内壁的同一个转轴，所述转轴的外壁固定套设有第二转板，所述第二转板的顶部固定连接有L型板，所述L型板远离马尔文Zeta电位样品池的一侧固定连接有拉簧，所述拉簧的另一端与安装槽的一侧内壁固定连接，所述L型板的顶部开设有卡槽。Preferably, the clamping assembly includes the same rotating shaft rotatably connected to the inner walls on both sides of the installation groove, the outer wall of the rotating shaft is fixedly sleeved with a second rotating plate, the top of the second rotating plate is fixedly connected with an L-shaped plate, the side of the L-shaped plate away from the Malvern Zeta potential sample cell is fixedly connected with a tension spring, the other end of the tension spring is fixedly connected with the inner wall of one side of the installation groove, and the top of the L-shaped plate is provided with a card slot.
优选地，所述固定组件包括固定连接在观测盒顶部的固定板，所述固定板的内部滑动贯穿连接有拉杆，所述拉杆远离马尔文Zeta电位样品池的一端固定连接有拉板，所述拉杆的另一端固定连接有挡板，所述固定板和挡板之间固定连接有同一个第一弹簧，所述第一弹簧套设在拉杆上，所述挡板的底部开设有三角槽。Preferably, the fixed assembly includes a fixed plate fixedly connected to the top of the observation box, the inside of the fixed plate slides through and is connected with a pull rod, the end of the pull rod away from the Malvern Zeta potential sample cell is fixedly connected with a pull plate, the other end of the pull rod is fixedly connected with a baffle plate, the same first spring is fixedly connected between the fixed plate and the baffle plate, the first spring is sleeved on the pull rod, and the bottom of the baffle plate is provided with a triangular groove.
优选地，所述安装槽的顶部内壁固定连接有对称设置的两个第二弹簧，所述第二弹簧的底部固定连接有支撑板，所述支撑板靠近马尔文Zeta电位样品池的一端固定连接有凸块，所述凸块的顶部滑动贯穿观测盒的顶部并固定连接有三角块，所述凸块的底部与卡槽相卡合，所述三角块与三角槽相卡合。Preferably, the top inner wall of the installation groove is fixedly connected with two symmetrically arranged second springs, the bottom of the second spring is fixedly connected with a support plate, and the end of the support plate near the Malvern Zeta potential sample cell is fixedly connected with a bump, the top of the bump slides through the top of the observation box and is fixedly connected with a triangular block, the bottom of the bump is engaged with the slot, and the triangular block is engaged with the triangular slot.
优选地，所述操作台的顶部固定连接有隔板，所述隔板的内部开设有通孔，所述隔板设置在激光器和衰减器之间，所述隔板靠近激光器的一侧设置有多个光线传感器。Preferably, a baffle is fixedly connected to the top of the console, and a through hole is opened inside the baffle, and the baffle is arranged between the laser and the attenuator, and a plurality of light sensors are arranged on the side of the baffle close to the laser.
优选地，所述第二检测器位于观测盒的一侧并与观测盒呈90度夹角，所述第一检测器位于观测盒的一侧并与观测盒呈173度夹角。Preferably, the second detector is located on one side of the observation box and forms an included angle of 90 degrees with the observation box, and the first detector is located at one side of the observation box and forms an included angle of 173 degrees with the observation box.
优选地，所述L型板靠近马尔文Zeta电位样品池的一端固定连接有橡胶块。Preferably, a rubber block is fixedly connected to one end of the L-shaped plate close to the Malvern Zeta potential sample cell.
与现有技术相比，本发明的有益效果是：Compared with prior art, the beneficial effect of the present invention is:
1、将马尔文Zeta电位样品池放置在观测盒内，马尔文Zeta电位样品池推动第二转板转动，第二转板带动L型板转动L型板带动橡胶块转动并拉伸拉簧，此时橡胶块与马尔文Zeta电位样品池的两侧相抵触，进而夹紧马尔文Zeta电位样品池；1. Place the Malvern Zeta potential sample cell in the observation box, the Malvern Zeta potential sample cell pushes the second rotating plate to rotate, the second rotating plate drives the L-shaped plate to rotate, the L-shaped plate drives the rubber block to rotate and stretches the tension spring, at this time the rubber block conflicts with both sides of the Malvern Zeta potential sample cell, and then clamps the Malvern Zeta potential sample cell;
2、支撑板在第二弹簧的弹力作用下竖直向下移动，支撑板带动凸块竖直向下移动，进而与卡槽卡合，凸块带动三角块竖直向下移动，此时挡板在第一弹簧的弹力作用下横向移动，进而挡板的底部与马尔文Zeta电位样品池的顶部相抵触，进而达到固定马尔文Zeta电位样品池的功能；2. The support plate moves vertically downward under the elastic force of the second spring, and the support plate drives the protrusion to move vertically downward, and then engages with the slot, and the protrusion drives the triangular block to move vertically downward, at this time, the baffle moves laterally under the elastic force of the first spring, and then the bottom of the baffle conflicts with the top of the Malvern zeta potential sample cell, thereby achieving the function of fixing the Malvern zeta potential sample cell;
3、在需要取出马尔文Zeta电位样品池时，用手横向拉动拉板，拉板带动拉杆横向移动，拉杆带动挡板横向移动并挤压第一弹簧，L型板在拉簧的拉力作用下转动，L型板带动第二转板转动，L型板推动凸块竖直向上移动，此时凸块的底部与L型板的顶部相抵触，第二转板的转动可以带动马尔文Zeta电位样品池竖直向上移动，便于取出马尔文Zeta电位样品池；3. When you need to take out the Malvern Zeta potential sample cell, pull the pull plate horizontally with your hand, the pull plate drives the pull rod to move horizontally, the pull rod drives the baffle to move horizontally and squeezes the first spring, the L-shaped plate rotates under the tension of the tension spring, the L-shaped plate drives the second rotating plate to rotate, and the L-shaped plate pushes the bump to move vertically upwards. At this time, the bottom of the bump conflicts with the top of the L-shaped plate. ;
4、在光线传感器检测到光线时，进而表明激光器已经倾斜，转动第一转板，第一转板带动螺杆转动，螺杆带动螺纹块竖直向上移动，进而调节激光器靠近隔板的一端的高度，直至光线传感器不能检测到光线，表明光线从隔板内水平射出。4. When the light sensor detects light, it indicates that the laser has been tilted. Turn the first rotating plate, the first rotating plate drives the screw to rotate, and the screw drives the threaded block to move vertically upwards, and then adjust the height of the end of the laser near the partition until the light sensor cannot detect the light, indicating that the light is emitted horizontally from the partition.
本发明中，通过夹紧组件的设置便于夹紧马尔文Zeta电位样品池，并且通过固定组件保证马尔文Zeta电位样品池的固定，避免样品池倾斜或晃动的情况，进而避免实验产生误差，横向拉动拉板即可取出马尔文Zeta电位样品池，隔板和光线传感器的设置保证激光器在实验时保持水平状态。In the present invention, the Malvern Zeta potential sample cell is conveniently clamped through the setting of the clamping component, and the fixing of the Malvern Zeta potential sample cell is ensured by the fixing component, avoiding the tilting or shaking of the sample cell, thereby avoiding errors in the experiment, and the Malvern Zeta potential sample cell can be taken out by pulling the pull plate laterally, and the arrangement of the partition and the light sensor ensures that the laser remains in a horizontal state during the experiment.
附图说明Description of drawings
图1为本发明提出的一种用于检测DNA变性的动态光散射检测装置的原理图；Fig. 1 is a schematic diagram of a dynamic light scattering detection device for detecting DNA denaturation proposed by the present invention;
图2为本发明提出的一种用于检测DNA变性的动态光散射检测装置的主视结构示意图；Fig. 2 is a schematic diagram of the main structure of a dynamic light scattering detection device for detecting DNA denaturation proposed by the present invention;
图3为本发明中观测盒的主视剖视结构示意图；Fig. 3 is the front view sectional structure schematic diagram of observation box in the present invention;
图4为本发明中A部分的放大图；Fig. 4 is the enlarged view of part A in the present invention;
图5为本发明中观测盒的主视结构示意图；Fig. 5 is the front view structure schematic diagram of observation box in the present invention;
图6为本发明中拉杆、拉板和挡板的连接图；Fig. 6 is the connection diagram of pull bar, pull plate and baffle plate among the present invention;
图7为本发明中隔板的主视剖视结构示意图。Fig. 7 is a schematic cross-sectional structure schematic diagram of a front view of a partition in the present invention.
图中：1、激光器；2、衰减器；3、观测盒；4、第一检测器；5、数字信号处理器；6、显示器；7、第二检测器；8、螺纹块；9、通孔；10、隔板；11、第一转板；12、螺杆；13、操作台；14、支撑杆；15、支撑柱；16、马尔文Zeta电位样品池；17、凸块；18、挡板；19、三角槽；20、三角块；21、第一弹簧；22、固定板；23、拉板；24、拉杆；25、第二弹簧；26、支撑板；27、拉簧；28、卡槽；29、安装槽；30、转轴；31、L型板；32、第二转板；33、橡胶块；34、光线传感器。In the figure: 1. laser; 2. attenuator; 3. observation box; 4. first detector; 5. digital signal processor; 6. display; 7. second detector; 8. threaded block; 9. through hole; 10. partition; 11. first rotating plate; 12. screw; 21, the first spring; 22, the fixed plate; 23, the pull plate; 24, the pull rod; 25, the second spring; 26, the support plate; 27, the extension spring;
具体实施方式Detailed ways
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.
实施例一Embodiment one
参照图1-7，一种用于检测DNA变性的动态光散射检测装置，包括操作台13和马尔文Zeta电位样品池16，操作台13的顶部设置有衰减器2、观测盒3、第一检测器4、数字信号处理器5、显示器6和第二检测器7，衰减器2和观测盒3均与激光器1设置在同一直线上，衰减器2位于激光器1和观测盒3之间，第一检测器4和第二检测器7均与数字信号处理器5电性连接，数字信号处理器5与显示器6电性连接，激光器1的下方设置有用于调节激光器1水平的调节组件，马尔文Zeta电位样品池16放置在观测盒3的内部，观测盒3的两侧内壁均开设有安装槽29，安装槽29的内部设置有用于夹紧马尔文Zeta电位样品池16的夹紧组件，观测盒3的顶部设置有对称设置的两个用于固定马尔文Zeta电位样品池16的固定组件，操作台13的底部四角均固定连接有支撑柱15。Referring to Fig. 1-7, a kind of dynamic light scattering detection device that is used to detect DNA denaturation, comprises console 13 and Malvern Zeta potential sample cell 16, and the top of console 13 is provided with attenuator 2, observation box 3, first detector 4, digital signal processor 5, display 6 and second detector 7, attenuator 2 and observation box 3 are all arranged on the same straight line with laser 1, attenuator 2 is positioned between laser 1 and observation box 3, first detector 4 and second detector 7 are all electrically connected with digital signal processor 5, digital The signal processor 5 is electrically connected to the display 6, and an adjustment component for adjusting the level of the laser 1 is provided under the laser 1. The Malvern Zeta potential sample cell 16 is placed inside the observation box 3, and the inner walls of both sides of the observation box 3 are provided with installation grooves 29. The interior of the installation groove 29 is provided with a clamping assembly for clamping the Malvern Zeta potential sample cell 16. The top of the observation box 3 is provided with two symmetrically arranged fixing components for fixing the Malvern Zeta potential sample cell 16. The four corners of the bottom of the console 13 are fixed A support column 15 is connected.
实施例二Embodiment two
本实施例在实施例一的基础上进行改进：调节组件包括固定连接在操作台13顶部的支撑杆14，支撑杆14的顶部与激光器1的底部转动连接，激光器1的底部转动连接有螺纹块8，螺纹块8的内部螺纹连接有螺杆12，螺杆12的底部转动贯穿操作台13并固定连接有第一转板11，夹紧组件包括转动连接在安装槽29两侧内壁的同一个转轴30，转轴30的外壁固定套设有第二转板32，第二转板32的顶部固定连接有L型板31，L型板31远离马尔文Zeta电位样品池16的一侧固定连接有拉簧27，拉簧27的另一端与安装槽29的一侧内壁固定连接，L型板31的顶部开设有卡槽28，固定组件包括固定连接在观测盒3顶部的固定板22，固定板22的内部滑动贯穿连接有拉杆24，拉杆24远离马尔文Zeta电位样品池16的一端固定连接有拉板23，拉杆24的另一端固定连接有挡板18，固定板22和挡板18之间固定连接有同一个第一弹簧21，第一弹簧21套设在拉杆24上，挡板18的底部开设有三角槽19。This embodiment is improved on the basis of Embodiment 1: the adjustment assembly includes a support rod 14 fixedly connected to the top of the operating table 13, the top of the supporting rod 14 is rotatably connected to the bottom of the laser 1, the bottom of the laser 1 is rotatably connected to a threaded block 8, the inner thread of the threaded block 8 is connected to a screw 12, the bottom of the screw 12 rotates through the operating table 13 and is fixedly connected to the first rotating plate 11, the clamping assembly includes the same rotating shaft 30 that is rotatably connected to the inner walls on both sides of the installation groove 29, and the outer wall of the rotating shaft 30 is fixed. The second rotating plate 32 is sleeved, and the top of the second rotating plate 32 is fixedly connected with an L-shaped plate 31. The side of the L-shaped plate 31 away from the Malvern Zeta potential sample cell 16 is fixedly connected with a tension spring 27. The other end of the tension spring 27 is fixedly connected with the inner wall of one side of the installation groove 29. The top of the L-shaped plate 31 is provided with a card slot 28. The fixed component includes a fixed plate 22 fixedly connected to the top of the observation box 3. One end of the a potential sample cell 16 is fixedly connected with a pull plate 23, the other end of the pull rod 24 is fixedly connected with a baffle plate 18, the same first spring 21 is fixedly connected between the fixed plate 22 and the baffle plate 18, the first spring 21 is sleeved on the pull rod 24, and the bottom of the baffle plate 18 is provided with a triangular groove 19.
实施例三Embodiment Three
本实施例在实施例一的基础上进行改进：安装槽29的顶部内壁固定连接有对称设置的两个第二弹簧25，第二弹簧25的底部固定连接有支撑板26，支撑板26靠近马尔文Zeta电位样品池16的一端固定连接有凸块17，凸块17的顶部滑动贯穿观测盒3的顶部并固定连接有三角块20，凸块17的底部与卡槽28相卡合，三角块20与三角槽19相卡合，操作台13的顶部固定连接有隔板10，隔板10的内部开设有通孔9，隔板10设置在激光器1和衰减器2之间，隔板10靠近激光器1的一侧设置有多个光线传感器34，第二检测器7位于观测盒3的一侧并与观测盒3呈90度夹角，第一检测器4位于观测盒3的一侧并与观测盒3呈173度夹角，L型板31靠近马尔文Zeta电位样品池16的一端固定连接有橡胶块33。This embodiment is improved on the basis of Embodiment 1: the top inner wall of the installation groove 29 is fixedly connected with two second springs 25 arranged symmetrically, the bottom of the second spring 25 is fixedly connected with a support plate 26, and one end of the support plate 26 close to the Malvern Zeta potential sample cell 16 is fixedly connected with a bump 17, the top of the bump 17 slides through the top of the observation box 3 and is fixedly connected with a triangular block 20, the bottom of the bump 17 is engaged with the slot 28, and the triangular block 20 is engaged with the triangular groove 19 The top of the console 13 is fixedly connected with a partition 10, the inside of the partition 10 is provided with a through hole 9, the partition 10 is arranged between the laser 1 and the attenuator 2, the side of the partition 10 close to the laser 1 is provided with a plurality of light sensors 34, the second detector 7 is located on the side of the observation box 3 and forms an angle of 90 degrees with the observation box 3, the first detector 4 is located at one side of the observation box 3 and forms an angle of 173 degrees with the observation box 3, and the L-shaped plate 31 is close to a side of the Malvern Zeta potential sample cell 16. The end is fixedly connected with a rubber block 33.
工作原理：在使用时，将马尔文Zeta电位样品池16放置在观测盒3内，马尔文Zeta电位样品池16推动第二转板32转动，第二转板32带动L型板31转动L型板31带动橡胶块33转动并拉伸拉簧27，此时橡胶块33与马尔文Zeta电位样品池16的两侧相抵触，进而夹紧马尔文Zeta电位样品池16，支撑板26在第二弹簧25的弹力作用下竖直向下移动，支撑板26带动凸块17竖直向下移动，进而与卡槽28卡合，凸块17带动三角块20竖直向下移动，此时挡板18在第一弹簧21的弹力作用下横向移动，进而挡板18的底部与马尔文Zeta电位样品池16的顶部相抵触，进而达到固定马尔文Zeta电位样品池16的功能，在需要取出马尔文Zeta电位样品池16时，用手横向拉动拉板23，拉板23带动拉杆24横向移动，拉杆24带动挡板18横向移动并挤压第一弹簧21，L型板31在拉簧27的拉力作用下转动，L型板31带动第二转板32转动，L型板31推动凸块17竖直向上移动，此时凸块17的底部与L型板31的顶部相抵触，第二转板32的转动可以带动马尔文Zeta电位样品池16竖直向上移动，便于取出马尔文Zeta电位样品池16，在光线传感器34检测到光线时，进而表明激光器1已经倾斜，转动第一转板11，第一转板11带动螺杆12转动，螺杆12带动螺纹块8竖直向上移动，进而调节激光器1靠近隔板10的一端的高度，直至光线传感器34不能检测到光线，表明光线从隔板10内水平射出。Working principle: when in use, the Malvern Zeta potential sample cell 16 is placed in the observation box 3, the Malvern Zeta potential sample cell 16 pushes the second rotating plate 32 to rotate, and the second rotating plate 32 drives the L-shaped plate 31 to rotate. The L-shaped plate 31 drives the rubber block 33 to rotate and stretches the tension spring 27. At this time, the rubber block 33 conflicts with the two sides of the Malvern Zeta potential sample cell 16, and then clamps the Malvern Zeta potential sample cell 16. The support plate 26 is under the elastic force of the second spring 25 Under the action, the support plate 26 drives the protrusion 17 to move vertically downward, and then engages with the card slot 28. The protrusion 17 drives the triangular block 20 to move vertically downward. At this time, the baffle 18 moves laterally under the elastic force of the first spring 21, and then the bottom of the baffle 18 conflicts with the top of the Malvern Zeta potential sample cell 16, thereby achieving the function of fixing the Malvern Zeta potential sample cell 16. When the Malvern Zeta potential sample cell 16 needs to be taken out, pull it laterally by hand The plate 23, the pull plate 23 drives the pull rod 24 to move laterally, the pull rod 24 drives the baffle plate 18 to move laterally and squeezes the first spring 21, the L-shaped plate 31 rotates under the tension of the extension spring 27, the L-shaped plate 31 drives the second rotating plate 32 to rotate, and the L-shaped plate 31 pushes the bump 17 to move vertically upwards. Take out the Malvern Zeta potential sample cell 16, and when the light sensor 34 detects the light, it indicates that the laser 1 has tilted, and the first rotating plate 11 is rotated, and the first rotating plate 11 drives the screw 12 to rotate, and the screw 12 drives the threaded block 8 to move vertically upwards, and then adjust the height of the end of the laser 1 close to the partition 10 until the light sensor 34 cannot detect the light, indicating that the light is emitted horizontally from the partition 10.
以上所述，仅为本发明较佳的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，根据本发明的技术方案及其发明构思加以等同替换或改变，都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention and its inventive concept to make equivalent replacements or changes, should be covered within the scope of protection of the present invention.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110357238.XA CN113029958B (en) | 2021-04-01 | 2021-04-01 | Dynamic light scattering detection device for detecting DNA denaturation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110357238.XA CN113029958B (en) | 2021-04-01 | 2021-04-01 | Dynamic light scattering detection device for detecting DNA denaturation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113029958A CN113029958A (en) | 2021-06-25 |
| CN113029958B true CN113029958B (en) | 2023-07-25 |
Family
ID=76454057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110357238.XA Expired - Fee Related CN113029958B (en) | 2021-04-01 | 2021-04-01 | Dynamic light scattering detection device for detecting DNA denaturation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113029958B (en) |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8705040B2 (en) * | 2004-03-06 | 2014-04-22 | Michael Trainer | Methods and apparatus for determining particle characteristics by measuring scattered light |
| US8697129B2 (en) * | 2011-03-02 | 2014-04-15 | Imra America, Inc. | Stable colloidal gold nanoparticles with controllable surface modification and functionalization |
| CN103748450A (en) * | 2011-08-19 | 2014-04-23 | 马尔文仪器有限公司 | Dual-mode characterization of particulates |
| CN103776802B (en) * | 2014-01-10 | 2016-06-08 | 上海理工大学 | The miniflow measuring viscoelastic fluid becomes measurement apparatus and method |
| US10119910B2 (en) * | 2015-10-09 | 2018-11-06 | Malvern Panalytical Limited | Particle characterisation instrument |
| US10365198B2 (en) * | 2016-04-21 | 2019-07-30 | Malvern Panalytical Limited | Particle characterization |
| DE202016006846U1 (en) * | 2016-11-07 | 2016-12-23 | Particle Metrix Gmbh | Device for measuring the concentration and size of nanoparticles in liquids in scattered light mode and in fluorescence mode |
| CN110987738A (en) * | 2019-12-26 | 2020-04-10 | 上海绿帝环保科技有限公司 | Automatic turbid liquid detection device and method |
| CN112067511A (en) * | 2020-10-10 | 2020-12-11 | 丹东百特仪器有限公司 | A kind of nanometer particle size analyzer based on backscattering cross-correlation technology and its detection method |
| CN112504922B (en) * | 2020-10-20 | 2022-09-02 | 华南师范大学 | Online measurement system and method for particle size distribution of atmospheric particulates |
-
2021
- 2021-04-01 CN CN202110357238.XA patent/CN113029958B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN113029958A (en) | 2021-06-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101820978A (en) | Dielectrophoresis device and method | |
| CN101294846A (en) | Shadowless lamp illumination detection device | |
| CN103163069A (en) | Method and system for measuring solid material surface adhesion force | |
| CN113029958B (en) | Dynamic light scattering detection device for detecting DNA denaturation | |
| CN207718072U (en) | A metallographic microscope polarization rotating disk adjustment device | |
| CN109410715A (en) | A kind of multifunctional physics teaching experimental rig | |
| CN219142634U (en) | Trace element detector | |
| CN101446540B (en) | Solid State NMR Characterization of Surface Hydrophobicity of Inorganic Nanomaterials | |
| CN210894363U (en) | Novel clinical laboratory blood analyzer | |
| CN212181204U (en) | LCD liquid crystal display detection device | |
| CN211553820U (en) | Sample testing arrangement for ornament | |
| CN209496521U (en) | A Physical Quantity Testing System | |
| CN114994893A (en) | Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal | |
| CN210664426U (en) | Roughness tester for surface treatment product | |
| CN221707234U (en) | Laser sedimentation instrument for measuring laboratory cleanliness | |
| CN221832143U (en) | A blood test separation device | |
| CN219737186U (en) | An automatic drop-type contact angle measuring instrument | |
| CN218121749U (en) | Calibration device of polarimeter | |
| CN207071421U (en) | A kind of medical science detection test tube stand | |
| CN213310300U (en) | Brain wave is data logging device for special attention | |
| CN205995489U (en) | Clinical blood sampling pipe holder | |
| CN215739070U (en) | A kind of medical ultrasonic diagnostic instrument probe bracket | |
| CN221925828U (en) | Smear ware is used in clinical medicine inspection | |
| RU2271734C2 (en) | Microelectrophoresis chamber | |
| CN219015971U (en) | Electronic halogen moisture tester |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20230725 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |