CN114994893A - Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal - Google Patents
Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal Download PDFInfo
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- CN114994893A CN114994893A CN202210597783.0A CN202210597783A CN114994893A CN 114994893 A CN114994893 A CN 114994893A CN 202210597783 A CN202210597783 A CN 202210597783A CN 114994893 A CN114994893 A CN 114994893A
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- submandibular gland
- air passage
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- 210000001913 submandibular gland Anatomy 0.000 title claims abstract description 79
- 241001465754 Metazoa Species 0.000 title claims abstract description 46
- 230000002438 mitochondrial effect Effects 0.000 title claims abstract description 14
- 238000001179 sorption measurement Methods 0.000 claims abstract description 56
- 239000011521 glass Substances 0.000 claims abstract description 14
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 230000033001 locomotion Effects 0.000 abstract description 14
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000011156 evaluation Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract 1
- 210000003470 mitochondria Anatomy 0.000 description 11
- 210000004907 gland Anatomy 0.000 description 9
- 210000003463 organelle Anatomy 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
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- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000003079 salivary gland Anatomy 0.000 description 2
- 238000010146 3D printing Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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Images
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The application discloses fixing device of living animal detection submaxillary gland mitochondrial morphology includes: the chassis is used for fixing the movable small animal; the bracket component is arranged on the chassis; the sucking disc assembly is arranged at the end part of the bracket assembly and is positioned above the chassis, the sucking disc assembly comprises a sucking disc and a glass slide, a sucking hole and a first air passage communicated with the sucking hole are formed in the sucking disc, and the first air passage is connected with negative pressure equipment; the glass slide is arranged at the upper end of the adsorption hole and seals the upper end of the adsorption hole; the lower end of the adsorption hole is used for adsorbing the submandibular gland of the small living animal and enabling the submandibular gland to be attached to the lower surface of the slide; and the microscope is arranged above the slide. The method and the device solve the problems that in the related art, when the submandibular gland of the small living animal is detected, the breathing and the heartbeat of the small living animal cause the focal plane to be continuously changed, so that motion artifacts are formed, and accurate recording and evaluation of mitochondrial forms, movement, functions and the like are seriously influenced.
Description
Technical Field
The application relates to the technical field of animal detection, in particular to a fixing device for detecting the mitochondrial morphology of submaxillary gland of a small living animal.
Background
Mitochondria are a major site of energy metabolism of salivary gland cells, and conventionally, examination of mitochondrial structure and function has been mainly focused on cell lines cultured ex vivo, but the mitochondrial morphology and function of ex vivo cells are greatly different from those of living bodies.
In recent years, researches are carried out on immersing mitochondria dye after exposing the submaxillary gland of a living mouse, fixing the animal under an inverted microscope in a prone mode through object compression, observing the movement, the shape and other changes of mitochondria in the submaxillary gland in a living state in real time, and realizing the imaging of the mitochondria in the submaxillary gland of the living small animal.
The research of the level of the organelles has high requirement on the detection accuracy, and because the submandibular gland is close to the heart and the lung, even slight breathing and heartbeat can cause the focal plane to change continuously when the small animal is imaged in a prone position, so that a motion artifact is formed, and the accurate recording and evaluation of the shape, movement, function and the like of mitochondria are seriously influenced.
Disclosure of Invention
The application mainly aims to provide a fixing device for detecting mitochondrial forms of submaxillary glands by small living animals, so as to solve the problems that in the related art, when the submaxillary glands of the small living animals are detected, the breathing and the heartbeat of the small living animals cause the focal plane to be continuously changed, so that motion artifacts are formed, and the accurate recording and evaluation of the mitochondrial forms, the movement, the functions and the like are seriously influenced.
In order to achieve the above object, the present application provides a fixing device for a small living animal to detect a mitochondrial morphology of a submandibular gland, the fixing device comprising:
the chassis is used for fixing the movable small animal;
the bracket component is arranged on the chassis;
the sucking disc assembly is arranged at the end part of the bracket assembly and is positioned above the base disc, the sucking disc assembly comprises a sucking disc and a glass slide, an adsorption hole and a first air passage communicated with the adsorption hole are formed in the sucking disc, and the first air passage is connected with negative pressure equipment;
the glass slide is arranged at the upper end of the adsorption hole and seals the upper end of the adsorption hole; the lower end of the adsorption hole is used for adsorbing the submandibular gland of the living small animal and enabling the submandibular gland to be attached to the lower surface of the slide;
and the microscope is arranged above the slide.
Further, the lower end of the adsorption hole is matched with the size of the submandibular gland, so that the submandibular gland covers the lower end of the adsorption hole.
Further, the suction cup comprises a disk part, a first handle part and a second handle part which are positioned at two ends of the disk part;
the adsorption hole is formed in the disc part, and the glass sheet is fixedly arranged at the upper end of the disc part and seals the upper end of the adsorption hole;
the first handle part is internally provided with the first air passage, and the second handle part is connected with the bracket component.
Further, a second air passage is arranged in the disc part and communicated with the first air passage;
the second air flue is located the outer periphery of absorption hole, the second air flue with through the third air flue intercommunication between the absorption hole, the third air flue is followed the circumference evenly distributed of second air flue to make the submandibular gland can receive even adsorption affinity.
Further, the first air passage is connected with a negative pressure device through an air pipe, and the negative pressure device can adjust the adsorption force of the adsorption hole on the submandibular gland.
Further, the glass slide is adhered to the upper end of the disc part; the slide and the adsorption hole are arranged coaxially.
Further, the bracket assembly comprises an upright post, a transverse and longitudinal adjusting bracket and a transverse post;
the upright post is fixedly arranged on the chassis, and the transverse and longitudinal adjusting bracket is arranged on the upright post and can be adjusted longitudinally;
the transverse column is arranged on the transverse and longitudinal adjusting frame and can be transversely adjusted, and one end of the sucking disc is connected with the transverse column.
Further, the transverse column can be transversely adjusted, longitudinally adjusted and rotatably adjusted through the transverse and longitudinal adjusting frame.
Further, the second handle part is detachably connected with the end part of the transverse column through threads.
In the embodiment of the application, the chassis is arranged for fixing the movable small animal; the bracket component is arranged on the chassis; the sucking disc assembly is arranged at the end part of the bracket assembly and is positioned above the chassis, the sucking disc assembly comprises a sucking disc and a glass slide, a sucking hole and a first air passage communicated with the sucking hole are formed in the sucking disc, and the first air passage is connected with negative pressure equipment; the glass slide is arranged at the upper end of the adsorption hole and seals the upper end of the adsorption hole; the lower end of the adsorption hole is used for adsorbing the submandibular gland of the small living animal and enabling the submandibular gland to be attached to the lower surface of the slide; the microscope is arranged above the slide, so that the aim of placing the small living animal on the base in a supine position, adsorbing the submaxillary gland tissue by the adsorption hole of the sucking disc and lifting the gland to be in a suspended state away from the body, and then detecting and observing the gland by the microscope is fulfilled, thereby realizing the normal function of the gland while fixing the gland, avoiding the influence of heartbeat and respiration on the focal plane under the microscope, obtaining the technical effect of obtaining a complete video image of the level of an organelle which can be continuously monitored, and further solving the problems that the breathing and heartbeat of the small living animal cause the focal plane to be continuously changed when detecting the submaxillary gland of the small living animal in the related technology, thereby forming motion artifacts and seriously influencing the accurate recording and evaluation of the mitochondrial morphology, the movement, the function and the like.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the application and to enable other features, objects, and advantages of the application to be more apparent. The drawings and their description illustrate the embodiments of the invention and do not limit it. In the drawings:
FIG. 1 is a schematic structural diagram according to an embodiment of the present application;
FIG. 2 is a schematic structural diagram of a chuck assembly according to an embodiment of the present application;
the device comprises a base plate 1, a vertical column 2, a transverse and longitudinal adjusting frame 3, a transverse column 4, a sucker assembly 5, a second handle portion 51, a first handle portion 52, a first air passage 53, a slide 54, a disc 55 portion 56, an adsorption hole 57, a second air passage 57, a sucker 58, a microscope 6, a submandibular gland 7, an air pipe 8, a negative pressure device 9 and a bracket assembly 10.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used.
In this application, the terms "upper", "lower", "inside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "disposed," "provided," "connected," "secured," and the like are to be construed broadly. For example, "connected" may be a fixed connection, a detachable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
In addition, the term "plurality" shall mean two as well as more than two.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.
In recent years, researches have been made on exposing the submandibular gland of a living mouse, soaking mitochondrial dye, fixing the animal in a prone mode under an inverted microscope through object compression, observing the movement, the shape and other changes of mitochondria in the submandibular gland in a living state in real time, and realizing the imaging of the mitochondria in the submandibular gland of the living small animal.
The research of the level of the organelles has high requirement on the detection accuracy, and because the submandibular gland is close to the heart and the lung, even slight breathing and heartbeat can cause the focal plane to be continuously changed when the small animal is imaged in the prone position, so that a motion artifact is formed, and the accurate recording and evaluation of the shape, movement, function and the like of mitochondria are seriously influenced.
As shown in fig. 1 to 2, an embodiment of the present application provides a fixing device for detecting mitochondria of a submandibular gland 7 of a small living animal, including:
the chassis 1 is used for fixing the movable small animal;
the bracket assembly 10 is arranged on the chassis 1;
the sucker assembly 5 is arranged at the end part of the bracket assembly 10 and is positioned above the chassis 1, the sucker assembly 5 comprises a sucker 58 and a slide 54, the sucker 58 is provided with an adsorption hole 56 and a first air passage 53 communicated with the adsorption hole 56, and the first air passage 53 is connected with the negative pressure equipment 9;
the slide 54 is provided at the upper end of the adsorption hole 56 and closes the upper end of the adsorption hole 56; the lower end of the adsorption hole 56 is used for adsorbing the submandibular gland 7 of the living small animal, and the submandibular gland 7 is attached to the lower surface of the slide 54;
the microscope 6 is provided above the slide 54.
In the present embodiment, the chassis 1 has a flat plate structure, and can be used as a mounting base for the rest of the components and for placing the living small animal. The small living animal may be placed on the base plate 1 in a supine position during examination, with the submandibular gland 7 of the small living animal exposed to the body surface. The rack assembly 10 is mounted on the chassis 1 as a mounting base for the chuck assembly 5 so that the chuck assembly 5 can be positioned over the chassis 1 and the live small animal. As shown in fig. 2, the suction cup assembly 5 is mainly composed of a suction cup 58 and a slide glass 54, the suction cup 58 functions to suck the submandibular gland 7 which has been exposed on the surface of the animal body, and the slide glass 54 functions to inspect and observe the submandibular gland 7 after the microscope 6 is mounted. Therefore, in the present embodiment, the suction pad 58 is provided with the suction hole 56 and the first air passage 53 communicating with the suction hole 56, and the negative pressure device 9 generates a negative pressure in the suction hole 56. Since the submandibular gland 7 needs to be adsorbed to the lower end of the adsorption hole 56 and thus the upper end of the adsorption hole 56 needs to be closed, this embodiment mounts the slide glass 54 on the upper end of the adsorption hole 56 and closes the upper end of the adsorption hole 56 so that the adsorption hole 56 can generate an adsorption force thereunder.
The height of the suction cup 58 can be adjusted to enable the adsorption hole 56 to be close to the submandibular gland 7, the submandibular gland 7 can be lifted up by using forceps and placed below the adsorption hole 56, the negative pressure device 9 is started to enable the submandibular gland 7 to be adsorbed on the adsorption hole 56 and to be evenly attached below the slide 54, and the submandibular gland 7 can be detected and observed through the microscope 6 and images can be obtained.
The embodiment achieves the purposes that the living small animal is placed on the base plate 1 in a supine posture, the absorption hole 56 of the suction disc 58 absorbs the tissue of the submandibular gland 7 and lifts the gland to be away from the body to be in a suspended state, and then the microscope 6 detects and observes the gland, thereby realizing the purpose that the normal function of the gland is not influenced while the gland is fixed, avoiding the influence of heartbeat and respiration on the focal plane under the microscope, obtaining the technical effect that the whole video image of the level of the organelle can be continuously monitored, and further solving the problems that the respiration and heartbeat of the living small animal can cause the constant change of the focal plane when the submandibular gland 7 of the living small animal is detected in the related technology, thereby forming motion artifacts and seriously influencing the accurate recording and evaluation of the mitochondrial morphology, the movement, the function and the like.
More specifically, C57/BL6 mice are taken as examples: mice had free access to food and water prior to the experiment, a day rhythm of 12 hours/12 hours, and a suction cup 58 matching the size of the mouse submandibular gland 7 was selected. Experiment the submandibular gland 7 was exposed after anaesthetising the small animals, the gland envelope was removed, the submandibular gland was soaked for 45 min with MitoTracker Deep Red (0.5 μ M), the dye was washed with normal saline and then wet-applied with gauze. Placing the small animal on the chassis 1, and adjusting the bracket assembly 10 to enable the suction cup 58 to be parallel to the ground and to be positioned about 0.5 cm above the submandibular gland 7; opening the negative pressure device 9, removing gauze on the submaxillary gland 7, supporting the submaxillary gland 7 by using a pair of tweezers, placing the submaxillary gland 7 below the suction disc 58, and after the negative pressure is formed between the adsorption hole 56 and the submaxillary gland 7, the submaxillary gland 7 is seen to be uniformly clung to the lower part of the slide 54, and the rest tissues of the submaxillary gland 7 are wet-dressed by using the gauze; after oil is dripped above the slide 54, the focal plane is adjusted, the microscope 6 can observe and shoot dynamic videos of mitochondria, and dynamic changes of the mitochondria can be recorded after further drug treatment. The microscope 6 can adopt a 63-fold oil lens of the single-photon microscope 6.
This application has following advantage:
the supine position of the small animal is adopted, the submandibular gland 7 is stably attached below the slide 54 through negative pressure suction, the submandibular gland 7 is not in contact with the body, the influence of breathing and heartbeat of the small animal on a focusing plane can be effectively avoided, the formation of artifacts is prevented, and the stable recording of horizontal pictures of organelles is facilitated; the secretion state of the salivary gland is affected by extrusion, the suction cup 58 in the embodiment can reduce the physical extrusion of the clamping and fixing device on the submandibular gland 7 to the maximum extent by regulating and controlling the negative pressure, and has no wound on the submandibular gland 7 tissue; the operation is simple and convenient, the measurement efficiency is high, the required wound is small, the fixing and detection time of the submaxillary gland 7 can be shortened, and the experimental result is easy to repeat; the detection cost is low, and the used device is easy to clean and can be reused.
In order to make the submandibular gland 7 stably stick to the lower surface of the slide 54, the lower end of the suction hole 56 is matched with the size of the submandibular gland 7 so that the submandibular gland 7 covers the lower end of the suction hole 56, and the whole suction cup 58 can be made of ABS resin by using 3D printing technology.
To facilitate the suction of the suction cup 58 to the submandibular gland 7 and the attachment to the bracket assembly 10, the suction cup 58 in this embodiment comprises a disc portion 55 and first and second handle portions 52, 51 at either end of the disc portion 55; an adsorption hole 56 is formed in the disc part 55, and a slide 54 is fixed to the upper end of the disc part 55 and closes the upper end of the adsorption hole 56; the first handle portion 52 has a first air passage 53 formed therein, and the second handle portion 51 is connected to the holder assembly 10.
In the present embodiment, the disk portion 55, the first shank portion 52, and the second shank portion 51 are integrally formed, the disk portion 55 has a flat structure, and the first shank portion 52 and the second shank portion 51 have a cylindrical structure and are located on both sides of the disk portion 55. The disc portion 55 is provided with suction holes 56 so that the submandibular gland 7 can be sucked and fixed to the lower end of the disc portion 55, and the slide 54 is fixed to the upper end of the disc portion 55. The first stem portion 52 has a straight first air passage 53 formed therein, and the first air passage 53 communicates with the adsorption hole 56. The first handle portion 52 may be connected to the negative pressure device 9 through a rubber tube so that the first air passage 53 communicates with the negative pressure device 9, and air in the adsorption hole 56 is drawn through the first air passage 53 so that negative pressure is formed in the adsorption hole 56.
In order for the submandibular gland 7 to be uniformly attached to the lower surface of the slide 54, it is necessary to subject the submandibular gland 7 to a uniform attaching force. For this purpose, the present embodiment is provided with a second air passage 57 in the disc portion 55, the second air passage 57 communicating with the first air passage 53; the second air passage 57 is located on the outer circumferential surface of the adsorption hole 56, the second air passage 57 is communicated with the adsorption hole 56 through a third air passage, and the third air passage is uniformly distributed along the circumferential direction of the second air passage 57, so that the submandibular gland 7 can be subjected to uniform adsorption force.
Since different living animals have different forms of the submandibular glands 7, in order to protect the submandibular glands 7 from being damaged, the adsorption force of the adsorption holes 56 needs to be adjusted for different submandibular glands 7, and therefore, in the embodiment, the first air duct 53 is connected to the negative pressure device 9 through the air duct 8, and the negative pressure device 9 can adjust the adsorption force of the adsorption holes 56 to the submandibular glands 7.
Since the height position of the suction cup 58 needs to be adjusted to bring the suction cup 58 close to the submandibular gland 7 when the living small animal is sucked to the submandibular gland 7, the bracket assembly 10 in this embodiment should have a function of adjusting the position of the suction cup 58. The bracket assembly 10 in this embodiment therefore comprises a vertical column 2, a transverse and longitudinal adjusting bracket 3 and a transverse column 4; the upright post 2 is fixedly arranged on the chassis 1, and the transverse and longitudinal adjusting frame 3 is arranged on the upright post 2 and can be adjusted longitudinally; the transverse column 4 is arranged on the transverse and longitudinal adjusting frame 3 and can be transversely adjusted, and one end of the sucking disc 58 is connected with the transverse column 4. The transverse column 4 can be transversely adjusted, longitudinally adjusted and rotationally adjusted through the transverse and longitudinal adjusting frame 3.
Specifically, horizontal vertical alignment jig 3 includes horizontal mounting groove and vertical mounting groove, through adjustable the installing on stand 2 of vertical mounting groove, through adjustable the installing on spreader 4 of horizontal mounting groove. The mounting positions of the upright post 2 and the cross post 4 can be locked by bolts. In order to make the rotation angle of the transverse column 4 adjustable as well, the transverse mounting groove is hinged on the longitudinal mounting groove in this embodiment, so that the transverse column 4 can rotate around the horizontal shaft. To facilitate replacement of the suction cup 58, which is sized to match the submandibular gland 7, the second handle portion 51 is in this embodiment removably attached to the end of the cross-post 4 by a screw thread.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (9)
1. A fixing device for detecting the mitochondrial morphology of submaxillary gland of a small living animal is characterized by comprising:
the chassis is used for fixing the movable small animal;
the bracket component is arranged on the chassis;
the sucking disc assembly is arranged at the end part of the bracket assembly and is positioned above the base disc, the sucking disc assembly comprises a sucking disc and a glass slide, an adsorption hole and a first air passage communicated with the adsorption hole are formed in the sucking disc, and the first air passage is connected with negative pressure equipment;
the glass slide is arranged at the upper end of the adsorption hole and seals the upper end of the adsorption hole; the lower end of the adsorption hole is used for adsorbing the submandibular gland of the living small animal and enabling the submandibular gland to be attached to the lower surface of the slide;
the bracket component comprises an upright post, a transverse and longitudinal adjusting bracket and a transverse post;
the upright post is fixedly arranged on the chassis, and the transverse and longitudinal adjusting bracket is arranged on the upright post and can be adjusted longitudinally;
the transverse column is arranged on the transverse and longitudinal adjusting frame and can be transversely adjusted, and one end of the sucking disc is connected with the transverse column.
2. The apparatus as claimed in claim 1, wherein the lower end of the suction hole is matched with the size of the submandibular gland so that the submandibular gland covers the lower end of the suction hole.
3. The apparatus as claimed in claim 1, wherein the suction cup includes a disk portion and first and second handle portions at both ends of the disk portion;
the adsorption hole is formed in the disc part, and the glass sheet is fixedly arranged at the upper end of the disc part and seals the upper end of the adsorption hole;
the first handle part is internally provided with the first air passage, and the second handle part is connected with the bracket component.
4. The apparatus according to claim 3, wherein a second air passage is provided in the disk portion, and the second air passage communicates with the first air passage;
the second air flue is located the outer periphery of absorption hole, the second air flue with through the third air flue intercommunication between the absorption hole, the third air flue is followed the circumference evenly distributed of second air flue to make the submandibular gland can receive even adsorption affinity.
5. The apparatus as claimed in claim 4, wherein the first air passage is connected to a negative pressure device through an air tube, and the negative pressure device can adjust the suction force of the suction hole to the submandibular gland.
6. The apparatus according to any one of claims 3 to 5, wherein the slide is attached to an upper end of the disk portion by adhesion.
7. The apparatus as claimed in claim 1, further comprising a microscope disposed above the slide.
8. The apparatus as claimed in claim 7, wherein the transverse column is laterally, longitudinally and rotationally adjustable by the transverse and longitudinal adjustment frame.
9. The apparatus as claimed in claim 3, wherein the second handle is detachably connected to the end of the transverse rod by a screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210597783.0A CN114994893A (en) | 2022-05-24 | 2022-05-24 | Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210597783.0A CN114994893A (en) | 2022-05-24 | 2022-05-24 | Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal |
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| CN114994893A true CN114994893A (en) | 2022-09-02 |
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| CN202210597783.0A Pending CN114994893A (en) | 2022-05-24 | 2022-05-24 | Fixing device for detecting mitochondrial morphology of submandibular gland by using small living animal |
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