CN112913611A - SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method - Google Patents
SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method Download PDFInfo
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- CN112913611A CN112913611A CN202110108209.XA CN202110108209A CN112913611A CN 112913611 A CN112913611 A CN 112913611A CN 202110108209 A CN202110108209 A CN 202110108209A CN 112913611 A CN112913611 A CN 112913611A
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 162
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 81
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 43
- 230000035764 nutrition Effects 0.000 title claims abstract description 43
- 238000003745 diagnosis Methods 0.000 title claims abstract description 36
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 title claims abstract description 28
- 235000021536 Sugar beet Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 28
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 75
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 75
- 230000012010 growth Effects 0.000 claims abstract description 18
- 239000000618 nitrogen fertilizer Substances 0.000 claims abstract description 16
- 238000005259 measurement Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 229930002875 chlorophyll Natural products 0.000 claims description 4
- 235000019804 chlorophyll Nutrition 0.000 claims description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 4
- 238000012417 linear regression Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 2
- 230000003698 anagen phase Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 6
- 239000003337 fertilizer Substances 0.000 abstract description 4
- 238000005070 sampling Methods 0.000 description 5
- 230000007547 defect Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 235000021537 Beetroot Nutrition 0.000 description 1
- DHHFDKNIEVKVKS-FMOSSLLZSA-N Betanin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC(C[C@H]2C([O-])=O)=C1[N+]2=C\C=C\1C=C(C(O)=O)N[C@H](C(O)=O)C/1 DHHFDKNIEVKVKS-FMOSSLLZSA-N 0.000 description 1
- DHHFDKNIEVKVKS-MVUYWVKGSA-N Betanin Natural products O=C(O)[C@@H]1NC(C(=O)O)=C/C(=C\C=[N+]/2\[C@@H](C(=O)[O-])Cc3c\2cc(O)c(O[C@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O2)c3)/C1 DHHFDKNIEVKVKS-MVUYWVKGSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 101000822633 Pseudomonas sp 3-succinoylsemialdehyde-pyridine dehydrogenase Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 235000012677 beetroot red Nutrition 0.000 description 1
- 239000001654 beetroot red Substances 0.000 description 1
- 235000002185 betanin Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/007—Determining fertilization requirements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of planting, and discloses a beet nitrogen nutrition diagnosis and topdressing decision method based on an SPAD value, which comprises the steps of carrying out beet planting, measuring the SPAD value of beet leaves, calculating the economic profit and marginal profit of the beet, determining the optimal nitrogen application amount and the SPAD value of the beet, and carrying out beet nitrogen nutrition diagnosis by comparing the actual measurement with the optimal SPAD value; establishing a quadratic regression equation between the SPAD value and the nitrogen application amount in the rapid growth period of the beet leaf cluster, calculating the actual fertilizing amount, and deciding whether to apply additional fertilizer or not and the using amount of the additional fertilizer through a beet nitrogen nutrition diagnosis and additional fertilizer model based on the SPAD value. Under the field test condition, the invention constructs a SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method, and provides technical parameters and a simple, convenient and feasible method for reasonably performing nitrogen nutrition diagnosis and topdressing nitrogen fertilizer in sugar beet production.
Description
Technical Field
The invention belongs to the technical field of planting, and relates to a method for diagnosing beet nitrogen nutrition and making topdressing decision based on an SPAD value.
Background
At present, beet is the second largest sugar crop in China and is widely planted in the North China, the northwest China and the northeast China. Nitrogen fertilizer is a nutrient element which has the greatest influence on the yield and quality of the beet, and the growth and development, the morphogenesis, the yield, the quality and the like of the beet are all influenced by nitrogen. In the production process, farmers pursue high-yield and blindly apply nitrogen fertilizer, which causes the problems of reduced utilization rate of the nitrogen fertilizer, reduced sugar content, pollution to land and water, and the like. Therefore, how to use the efficient and simple nitrogen nutrition diagnosis technology to guide scientific and reasonable application of nitrogen fertilizers and promote yield and quality improvement of beet is one of the problems faced by beet production at present.
Nitrogen nutrition is a key factor influencing the yield and sugar content improvement of the beet, the nitrogen is a main component of nucleic acid, phospholipid and protein in the cell protoplasm, the nitrogen is also an indispensable element in metabolic enzyme of the beet and is one of chlorophyll compositions. Numerous studies have shown that both too high and too low nitrogen application reduces the yield and sugar yield of sugar beets. Therefore, in order to judge the nitrogen nutrition condition of the beet, especially to find out the deficiency of the beet to the nitrogen nutrition as the basis for additional fertilization, a detection instrument and a method for monitoring the nitrogen nutrition level simply, quickly, accurately and nondestructively are urgently needed.
The SPAD instrument is used as one of nondestructive nitrogen nutrition diagnosis technologies, has the characteristics of high precision, low price and simple operation, and can be popularized and applied to various crops, and all people can master the measurement skills after training. However, few research reports are reported for carrying out nitrogen nutrition diagnosis on beet by utilizing a SPAD instrument at present, and a recommended nitrogen fertilizer topdressing model is not established. The nitrogen nutrition diagnosis of beet by SPAD instrument is not applied in beet production.
Through the above analysis, the problems and defects of the prior art are as follows: at present, no method for diagnosing nitrogen nutrition of beet in each growth period by using SPAD instrument exists, and no recommended timely nitrogen fertilizer topdressing model is established.
The difficulty in solving the above problems and defects is: establishing the beet nitrogen nutrition diagnosis parameters based on the SPAD value requires a large amount of field tests, and synchronously obtaining the SPAD value, the plant nitrogen content, the yield and the sugar content of the beet in a test cell.
The significance of solving the problems and the defects is as follows: the invention constructs a beet nitrogen nutrition diagnosis system and a topdressing model based on the SPAD value, and provides an effective method for reasonably carrying out nitrogen nutrition diagnosis and topdressing nitrogen fertilizer decision in beet production.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for diagnosing and topdressing beet nitrogen nutrition based on SPAD value.
The invention is realized in such a way that a SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method comprises the following steps:
planting beet, and recording the nitrogen fertilizer application amount in the growth period of the beet;
step two, obtaining beet samples in each growth period of the beet; measuring the hammered value of the obtained fresh beet root sample by using a sugar measuring instrument, and weighing the obtained beet sample by using an electronic scale;
step three, measuring the SPAD value of the living beet leaves in a test field by using a portable chlorophyll meter SPAD-502;
step four, calculating the economic profit and marginal profit of the beet when the beet is harvested; determining the optimal nitrogen application amount and SAPD value of the beet based on the nitrogen application amount, nitrogen content, sugar content, yield and sugar yield of the beet and the economic profit and marginal profit of the beet;
establishing a quadratic curve relation between the SPAD value and the nitrogen application amount of the beet in each breeding period based on the SPAD value of the beet in each breeding period and the acquired nitrogen application amount data; establishing a beet nitrogen nutrition diagnosis and topdressing model based on the SPAD value based on the quadratic curve relationship between the SPAD value and the nitrogen application amount of each breeding period of the beet.
Further, in step one, the obtaining of the sugar beet sample comprises:
the 6 sampling times are as follows: sampling for the first time when the beet is at the seedling stage of 40 days, and then sampling once every 20 days, namely 1 time at the seedling stage, 2 times at the rapid growth stage of the leaf cluster, 2 times at the root sugar increment stage and 1 time at the sugar accumulation stage;
the sampling method comprises the following steps: selecting a region with uniform growth vigor in a beet test cell, randomly selecting 5 plants, separating the whole beet according to roots, petioles and leaves, and cutting the root tuber of the beet into small blocks with the same size; is divided into two parts, one part is put into a sterile bag for freezing and fresh keeping, and the other part is dried in the shade and then dried and crushed.
Further, in the second step, the sugar content calculation formula is as follows: the sugar content (%). percent is a weight value × 0.85.
Further, in step three, the determination of the SPAD value of the beet leaves comprises: measuring SPAD values of leaf tips, leaves and leaf margins of the inverted beet three leaves, measuring 5 plants in each region, taking 1 leaf from each plant, and repeating the steps for three times.
Further, in the fourth step, the optimum nitrogen application amount of the beet is the corresponding nitrogen application amount when the maximum profit and the marginal profit are 0.
Further, in step five, the SPAD value-based beet nitrogen nutrition diagnosis and topdressing model comprises the following steps:
in the formula, NsIndicating the amount of nitrogen fertilizer to be applied in the beet growth period; n is a radical ofoptRepresents the optimal nitrogen application amount in the growth period; c represents the intercept of the linear regression equation, b represents the first order regression coefficient of the equation, and a represents the second order regression coefficient of the equation.
Another object of the present invention is to provide a method for improving the utilization efficiency of nitrogen fertilizer by using the sugar beet nitrogen nutrition diagnosis and topdressing model.
By combining all the technical schemes, the invention has the advantages and positive effects that: under the field test condition, the invention constructs a SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method, and provides technical parameters and a simple, convenient and feasible method for reasonably performing nitrogen nutrition diagnosis and topdressing nitrogen fertilizer in sugar beet production.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a method for diagnosing and making topdressing decisions of betanin based on SPAD value provided by the embodiment of the invention.
FIG. 2 is a schematic diagram of a position for measuring the SPAD value of the blade according to the embodiment of the invention.
FIG. 3 is a graph showing SPAD values of leaves of 3 sugar beet varieties under different nitrogen treatments according to the present invention.
FIG. 4 is a graph showing the nitrogen content of the leaf cluster and root of sugar beet at different nitrogen applications according to the present invention.
FIG. 5 is a graphical representation of the profit of 3 sugar beet varieties at different nitrogen rations provided by the examples of the present invention.
FIG. 6 is a graph showing the marginal gain of 3 sugar beet varieties at different nitrogen application rates provided by an example of the present invention.
FIG. 7 is a graph showing the relationship between the SPAD value and nitrogen application of sugar beet according to the example of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for diagnosing and making topdressing decisions of beet nitrogen nutrition based on SPAD value, which is described in detail with reference to the accompanying drawings.
As shown in fig. 1, the SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method provided by the embodiment of the invention comprises the following steps:
s101, planting the beet, and recording the nitrogen fertilizer application amount of the beet in each growth period;
s102, measuring the SPAD value of the beet leaves by using a portable chlorophyll meter SPAD-502;
s103, comparing the actually measured SPAD value with the optimal SPAD value, and carrying out beet nitrogen nutrition diagnosis;
s104, calculating the actual fertilizing amount by establishing a quadratic regression equation between the SPAD value and the nitrogen application amount of each growth period of the beet;
and S105, establishing a recommended fertilizing amount through an optimal fertilizing amount and a beet nitrogen nutrition diagnosis and additional fertilization model based on the SPAD value.
In step S101, the obtaining of the sugar beet sample provided by the embodiment of the present invention includes: the rapid growth period of the leaf bundle is 2 times;
in step S102, the SPAD value determination parts of the beet leaves implemented by the invention are as follows: inverting the leaf tips of 3 leaves;
in step S103, the determining the SPAD value of the sugar beet leaves provided by the embodiment of the present invention includes: more than 15 plants were measured per area.
In step S104, the optimum nitrogen application amount of the sugar beet provided by the embodiment of the present invention is the corresponding nitrogen application amount when the maximum profit and the marginal profit are 0.
In step S105, the SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing model provided in the embodiment of the present invention includes:
(1) the optimal nitrogen application amount range of high yield and high sugar is as follows: 177-180kg/ha
(2) The optimal SPAD value range of high yield and high sugar is as follows: 46.6-56.9
(3) Secondary regression equation of SPAD value and nitrogen application amount:
60-day-old Y-43.7296 +0.0406X-9.3874X10-5X2
Y-53.8311 +0.0270X-5.4838X10 for 80 days of seedling age-5X2
(4) Nitrogen dressing model:
in the formula, NsIndicating the amount of nitrogen fertilizer applied in each growth period; n is a radical ofoptRepresents the optimal nitrogen application amount in one fertility period; c represents the intercept of the equation, b represents the first order regression coefficient of the equation, and a represents the second order regression coefficient of the equation.
In order to facilitate practical application, a simple model algorithm is obtained based on C language programming.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method is characterized by comprising the following steps:
planting beet, and measuring the SPAD value of beet leaves by using a portable chlorophyll meter SPAD-502;
establishing a quadratic regression equation between the SPAD value and the nitrogen application amount of each growth period of the beet based on the SPAD value of each growth period of the beet leaves and the acquired nitrogen application amount data; establishing a nitrogen nutrition diagnosis and topdressing model based on the SPAD value for the beet based on a quadratic curve relation between the SPAD value and the nitrogen application amount in each breeding period of the beet for nitrogen nutrition diagnosis and topdressing decision.
2. The SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method of claim 1, wherein the obtaining of sugar beet samples comprises:
the measurement was performed 2 times at the fast growth phase of the leaf cluster with the largest nitrogen demand of sugar beet.
3. The SPAD value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method of claim 1, wherein the determining SPAD values of sugar beet leaves comprises: the SPAD value of the leaf tip of the inverted beet three leaves was measured, and 15 or more plants were measured per area.
4. The SPAD value-based beet nitrogen nutrition diagnosis and topdressing decision method as claimed in claim 1, wherein the optimum nitrogen application of beet is the corresponding nitrogen application when the maximum profit and marginal profit are 0.
5. The SPAD-value-based sugar beet nitrogen nutrition diagnosis and topdressing decision method according to claim 1, wherein the SPAD-value-based sugar beet nitrogen nutrition diagnosis and topdressing model comprises:
in the formula, NsIndicating the amount of nitrogen fertilizer applied in the growth period; n is a radical ofoptRepresents the optimal nitrogen application amount in the growth period; c represents the intercept of the linear regression equation, b represents the first order regression coefficient of the equation, and a represents the second order regression coefficient of the equation.
6. A beet nitrogen nutrition diagnosis and topdressing model constructed by the SPAD value-based beet nitrogen nutrition diagnosis and topdressing decision method according to any one of claims 1-5.
7. A method for promoting yield and quality increase of beet, which is characterized in that the method for promoting yield and quality increase of beet uses the beet nitrogen nutrient diagnosis and top dressing model as claimed in claim 6.
8. A method for improving nitrogen fertilizer utilization efficiency, which is characterized in that the method for improving nitrogen fertilizer utilization efficiency uses the beet nitrogen nutrition diagnosis and topdressing model of claim 6.
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CN101904263A (en) * | 2010-07-21 | 2010-12-08 | 湖北省农业科学院植保土肥研究所 | Accuracy control method of nitrate content in Chinese cabbage |
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CN109392398A (en) * | 2018-11-20 | 2019-03-01 | 内蒙古农业大学 | A kind of potato nitrogen fertilizer recommendation method that soil testing is combined with plant diagnosis |
WO2020013736A1 (en) * | 2018-07-11 | 2020-01-16 | Общество с ограниченной ответственностью "АгроДронГрупп" | Remote investigative diagnosis of nitrogen supply to crops |
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2021
- 2021-01-27 CN CN202110108209.XA patent/CN112913611A/en active Pending
Patent Citations (4)
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CN101904263A (en) * | 2010-07-21 | 2010-12-08 | 湖北省农业科学院植保土肥研究所 | Accuracy control method of nitrate content in Chinese cabbage |
CN104584751A (en) * | 2014-12-20 | 2015-05-06 | 华中农业大学 | Fertilizing method based on nitrogen nutrition nondestructive detection of winter rapes |
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Non-Patent Citations (4)
Title |
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