CN112896737B - Detect BRAF gene mutation detect reagent storage device - Google Patents

Detect BRAF gene mutation detect reagent storage device Download PDF

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Publication number
CN112896737B
CN112896737B CN202110151412.5A CN202110151412A CN112896737B CN 112896737 B CN112896737 B CN 112896737B CN 202110151412 A CN202110151412 A CN 202110151412A CN 112896737 B CN112896737 B CN 112896737B
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China
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liquid
reagent
cooling
bin
liquid outlet
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CN202110151412.5A
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CN112896737A (en
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王萍
付浴东
于清
邓玉杰
王青
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Affiliated Hospital of University of Qingdao
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Affiliated Hospital of University of Qingdao
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/02Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
    • B65D81/05Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient

Abstract

The invention discloses a BRAF gene mutation detection reagent storage device, wherein a cooling liquid is used as a temperature maintaining medium of a reagent tube, the stability of temperature maintaining of the reagent tube can be effectively improved, the cooling liquid in a liquid changing and cooling component can be monitored in real time through a temperature sensor, when the liquid is synchronously changed, the cooler cooling liquid in a circulating pipeline can be supplemented into a liquid changing groove through the suction of a miniature suction pump, and part of the cooling liquid in the liquid changing groove is discharged, so that the cooling capacity of the cooling liquid in the liquid changing groove can be stably improved to reach the initial level, in addition, after the cooling liquid in the circulating pipeline enters the liquid changing groove, a push plate can be pushed to move, the liquid in the liquid changing groove can be pushed out by the push plate, and a distance is arranged between the top of the push plate and the top of the liquid changing groove, so that after the liquid changing is finished, the push plate resets automatically, has improved and has traded liquid efficiency, has guaranteed the validity of reagent, and then has guaranteed the accuracy that detects.

Description

Detect BRAF gene mutation detect reagent storage device
Technical Field
The invention relates to the technical field of reagent storage, in particular to a storage device for a detection reagent for detecting BRAF gene mutation.
Background
The BRAF gene is located on chromosome 7 and is an oncogene, wherein V600E is a site which is most prone to mutation in the BRAF gene, and is often mutated in melanoma, non-Hodgkin lymphoma, colorectal cancer, thyroid cancer and non-small cell lung cancer, so that the BRAF gene is most commonly applied to auxiliary diagnosis of thyroid cancer at present, corresponding detection reagents are required during gene detection, the reagents are required to be stored and used, the reagents are often directly placed in a refrigerated cabinet at present, the heat preservation efficiency and the effect of the reagents are difficult to guarantee, and in the transportation process, the reagents cannot be protected for a reagent tube.
Therefore, it is necessary to provide a storage device for a detection reagent for detecting BRAF gene mutation to solve the above problems in the background art.
Disclosure of Invention
In order to achieve the purpose, the invention provides the following technical scheme: a detect BRAF gene mutation detect reagent storage device which characterized in that: the reagent tube storage device comprises a storage tank, a circulating pipeline, a refrigerating bin, a circulating pump, a liquid changing and cooling component and a reagent tube placing component, wherein the circulating pipeline is fixedly arranged below the inner part of the storage tank and comprises an upper horizontal pipeline and a lower horizontal pipeline, the refrigerating bin is embedded and communicated in the lower horizontal pipeline, and cooling liquid in the circulating pipeline is driven by the circulating pump to circularly flow;
go up among the horizontal pipeline along coolant flow direction intercommunication in proper order and have at least two sets of liquid and the cooling subassembly of trading, trade liquid and cooling subassembly and can place the subassembly for the reagent pipe and provide the coolant liquid that the temperature is suitable, the reagent pipe is placed the subassembly and is used for placing the reagent pipe.
Further, as preferred, trade liquid and cooling subassembly includes feed liquor pipe, miniature suction pump, trades the cistern, drain pipe and divides the liquid storehouse, wherein, the one end of feed liquor pipe is connected to in the last horizontal pipeline, and the other end communicates to in trading the cistern, the one end intercommunication that trades the cistern and keeps away from the feed liquor pipe has the drain pipe, the other end of drain pipe communicates to in the last horizontal pipeline, install the miniature suction pump that can pump the coolant liquid to in trading the cistern from last horizontal pipeline in the feed liquor pipe, the top of trading the cistern is fixed to be communicated with and is divided the liquid storehouse, divide the liquid storehouse to provide the coolant liquid for reagent pipe places the subassembly.
Further, as an optimization, a one-way valve mechanism is installed in the liquid outlet pipe, the one-way valve mechanism comprises a liquid outlet bin, a liquid outlet and a liquid blocking ball, wherein a conical liquid outlet is formed in one side of the liquid outlet bin, the outer portion of the liquid outlet is connected with the liquid outlet pipe, the liquid blocking ball is placed in the liquid outlet, the liquid blocking ball is fixed at the end portion of the piston rod, the other end of the piston rod is arranged in the cylinder body in a sealing and sliding mode, the cylinder body is fixed at one end, far away from the liquid outlet, of the liquid outlet bin, the end, far away from the liquid outlet, of the piston rod is connected with the cylinder body through a reset spring, a plurality of through holes are further distributed in one side, far away from the liquid outlet, of the liquid outlet bin, and the through holes are coated by the liquid outlet pipe;
the elasticity of the return spring can ensure that the reagent tube placing assembly is filled with cooling liquid and the liquid blocking ball is driven to abut against the liquid outlet after the micro suction pump stops working.
Further, as preferred, the inside in branch liquid storehouse is fixed with the filter disc, the filter disc is the cavity structure, the outside fixed cover of filter disc is equipped with the annular and keeps off the stopper, it has a plurality of pipes of keeping in to array on the outer circumference of stopper, the one end of pipe of keeping in is for sealing, and the other end passes and keeps off the stopper and stretch into in the filter disc.
Further, preferably, a plurality of positive and negative rotating motors are arrayed on the outer circumference of the liquid separating bin, and a reagent tube placing assembly is fixed at the output end of each positive and negative rotating motor.
Further, as preferred, the reagent pipe placing assembly includes a liquid distribution disc, a placing pipe and a wrapping bag, wherein the placing pipe is circumferentially arrayed and fixedly embedded in the liquid distribution disc, a tubular wrapping bag is adhered to the inner side wall and the bottom wall of the placing pipe, the wrapping bag is communicated with the liquid distribution disc through a pipe body, so that the wrapping bag is filled with cooling liquid, and the liquid distribution disc is communicated with the temporary storage pipe through an elastic corrugated pipe.
Further, as preferred, it is provided with the push pedal to slide in the liquid changing groove, the push pedal adopts the spring to link to each other with the lateral wall in liquid changing groove, just there is the interval at the top of push pedal and the top in liquid changing groove.
Further, as preferred, be provided with on the bin and trade the corresponding material door mechanism of getting of liquid and cooling subassembly, get the material door mechanism and include the carousel, get material mouth, sealing baffle and articulated seat, wherein, the carousel rotates and sets up on the bin, just the rotation central axis of carousel coincides with the array axis of a plurality of positive and negative motor that changes, it gets the material mouth to have seted up on the carousel, it is corresponding with the position that the subassembly was placed to the reagent pipe to get the material mouth, just the top of getting the material mouth is provided with sealing baffle, sealing baffle's one side articulates on articulated seat, articulated seat is fixed on the bin.
Further, preferably, a semiconductor refrigerator and a temperature sensor are fixedly embedded in the liquid separation chamber.
Further preferably, the system also comprises a controller which uniformly controls the semiconductor refrigerator, the temperature sensor forward and reverse rotation motor, the micro suction pump and the circulating pump.
Compared with the prior art, the invention provides a storage device for a detection reagent for detecting BRAF gene mutation, which has the following beneficial effects:
in addition, the reagent tube can be effectively refrigerated by the omnibearing package, so that the reagent tube can be stored in the longest storage time, and the stability of temperature maintenance of the reagent tube can be effectively improved;
the temperature sensor can monitor the cooling liquid in the liquid changing and cooling assembly in real time, and when the liquid is changed synchronously, the micro suction pump can suck the relatively cold cooling liquid in the circulating pipeline to the liquid changing tank and discharge part of the cooling liquid in the liquid changing tank, so that the cooling capacity of the cooling liquid in the liquid changing tank can be stably improved, and the cooling liquid reaches the initial level;
in addition, after coolant liquid in the circulating line enters into the liquid changing groove, the push plate can be pushed to move, the liquid in the liquid changing groove can be pushed out by the push plate, and the distance is arranged between the top of the push plate and the top of the liquid changing groove, so that the push plate can be ensured to be automatically reset after liquid changing is finished, the liquid changing efficiency is improved, the effectiveness of a reagent is ensured, and the accuracy of BRAF gene mutation detection is further ensured.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a partially enlarged schematic view of FIG. 1;
FIG. 3 is a schematic structural view of a material taking door mechanism according to the present invention;
FIG. 4 is a schematic structural view of a check valve mechanism according to the present invention;
in the figure: 1. a storage tank; 2. a circulation pipe; 3. a refrigeration bin; 4. a circulation pump; 5. a liquid changing and cooling component; 6. a positive and negative rotation motor; 7. a reagent tube placement assembly; 8. a material taking door mechanism; 51. a liquid inlet pipe; 52. a micro suction pump; 53. a liquid changing tank; 54. a liquid outlet pipe; 55. a one-way valve mechanism; 56. pushing the plate; 57. a liquid separation bin; 58. a filter tray; 59. blocking the plug; 510. a temporary storage tube; 511. a semiconductor refrigerator; 71. a liquid separating disc; 72. placing a tube; 73. wrapping the bag; 81. a turntable; 82. a material taking port; 83. sealing the baffle; 84. a hinged seat; 551. a liquid outlet bin; 552. a liquid outlet; 553. a through hole; 554. a liquid blocking ball; 555. a cylinder body; 556. a piston rod; 557. a return spring.
Detailed Description
Referring to fig. 1 to 4, in an embodiment of the present invention, a BRAF gene mutation detection reagent storage device includes a storage tank 1, a circulation pipeline 2, a refrigeration bin 3, a circulation pump 4, a liquid change and cooling component 5, and a reagent tube placement component 7, wherein the circulation pipeline 2 is fixedly disposed below the interior of the storage tank 1, the circulation pipeline 2 includes an upper horizontal pipeline and a lower horizontal pipeline, the refrigeration bin 3 is embedded and communicated in the lower horizontal pipeline, and a cooling liquid in the circulation pipeline 2 is driven by the circulation pump 4 to flow in a circulation manner;
go up in the horizontal pipeline along coolant flow direction in proper order the intercommunication have at least two sets of liquid and the cooling subassembly 5 of trading, trade liquid and cooling subassembly 5 can place subassembly 7 for the reagent pipe and provide the suitable coolant liquid of temperature, the reagent pipe is placed subassembly 7 and is used for placing the reagent pipe to the coolant liquid keeps the medium as the temperature of reagent pipe, stability that the temperature that can effectual improvement reagent pipe kept.
In this embodiment, as shown in fig. 2, the liquid exchanging and cooling assembly 5 includes a liquid inlet pipe 51, a micro-suction pump 52, a liquid exchanging tank 53, a liquid outlet pipe 54 and a liquid dividing chamber 57, wherein one end of the liquid inlet pipe 51 is connected to the upper horizontal pipe, the other end is communicated to the liquid exchanging tank 53, one end of the liquid exchanging tank 53 away from the liquid inlet pipe 51 is communicated with the liquid outlet pipe 54, the other end of the liquid outlet pipe 54 is communicated to the upper horizontal pipe, the liquid inlet pipe 51 is installed with the micro-suction pump 52 capable of sucking the cooling liquid from the upper horizontal pipe to the liquid exchanging tank 53, the liquid dividing chamber 57 is fixedly communicated above the liquid exchanging tank 53, the liquid dividing chamber 57 can provide the cooling liquid for the reagent pipe placing assembly 7, the relatively cold cooling liquid in the circulating pipe 2 can be supplemented to the liquid exchanging tank 53 by the suction of the micro-suction pump, and part of the cooling liquid in the liquid exchanging tank 53 can be discharged, thereby, the cooling capacity of the cooling liquid in the liquid change tank 53 can be smoothly raised to reach the initial level.
Further, a one-way valve mechanism 55 is installed in the liquid outlet pipe 54, the one-way valve mechanism 55 includes a liquid outlet bin 551, a liquid outlet 552 and a liquid blocking ball 554, wherein a tapered liquid outlet 552 is installed at one side of the liquid outlet bin 551, the outside of the liquid outlet 552 is connected with the liquid outlet pipe 54, the liquid blocking ball 554 is placed inside the liquid outlet, the liquid blocking ball 554 is fixed at the end of a piston rod 556, the other end of the piston rod 556 is hermetically and slidably arranged in a cylinder 555, the cylinder 555 is fixed at one end of the liquid outlet bin 551 far from the liquid outlet 552, one end of the piston rod 556 far from the liquid outlet 552 is connected with the cylinder by a return spring 557, a plurality of through holes 553 are further distributed at one side of the liquid outlet bin 551 far from the liquid outlet 552, and the through holes 553 are covered by the liquid outlet pipe 54;
the elastic force of the return spring 557 can ensure that the reagent tube placing assembly 7 is filled with the cooling liquid and the liquid blocking ball 554 is driven to block the liquid outlet 552 after the micro-suction pump 52 stops working.
In addition, divide the inside of liquid storehouse 51 to be fixed with filter disc 58, filter disc 58 is the cavity structure, the outside fixed cover of filter disc 58 is equipped with the annular and keeps off stopper 59, it has a plurality of pipe 510 of keeping in to arrange on the outer circumference of stopper 59, the one end of pipe 510 of keeping in is for sealing, and the other end passes and keeps off stopper 59 and stretch into filter disc 58, enters into the coolant liquid that divides in the liquid storehouse 51, if need enter into to parcel bag 73 in, then need pass through the filtration of filter disc 58, and filter disc 58 can filter the particulate matter in the coolant liquid, prevents that the particulate matter from entering into to parcel bag 73 to damage the reagent pipe.
As a preferred embodiment, a plurality of positive and negative rotation motors 6 are arrayed on the outer circumference of the liquid separation chamber 57, and a reagent tube placing assembly 7 is fixed at the output end of each positive and negative rotation motor 6, so that on one hand, the positive and negative rotation motors 6 periodically rotate to drive the reagent tube placing assembly 7 to rotate, and thus reagent deposition can be prevented, and on the other hand, when reagent tubes are taken out, the positive and negative rotation motors 6 can drive the corresponding reagent tubes to rotate to the position of the material taking port 82.
In this embodiment, as shown in fig. 2, the reagent tube placing assembly 7 includes a liquid distribution tray 71, placing tubes 72 and a packing bag 73, wherein a plurality of the placing tubes 72 are fixedly embedded in the liquid distribution tray 71 in a circumferential array manner, and the tubular packing bag 73 is adhered to the inner side wall and the bottom wall of the placing tubes 72 together, the packing bag 73 is communicated with the liquid distribution tray 71 through a tube body, so that the packing bag 73 is filled with a cooling liquid, the liquid distribution tray 71 is communicated with the temporary storage tube 510 through an elastic corrugated tube, and the packing bag 73 is filled with the cooling liquid, so that the reagent tubes can be well buffered, the reagent tubes can be effectively packed and protected, and in addition, the packing can better provide refrigeration for the reagent tubes, so that the reagent tubes can be stored in a longest storage time in an all-directional manner.
As a preferred embodiment, the liquid changing tank 53 is slidably provided with a push plate 56, the push plate 56 is connected to a side wall of the liquid changing tank 53 by a spring, and there is a gap between a top of the push plate 56 and a top of the liquid changing tank 53, when the cooling liquid in the circulation pipeline 2 enters the liquid changing tank 53, the push plate 56 is pushed to move, the push plate 56 can push out the liquid in the liquid changing tank 53, and there is a gap between the top of the push plate 56 and the top of the liquid changing tank 53, so that it can be ensured that the push plate 56 automatically resets after the liquid changing is finished, specifically, the cooling liquid in the circulation pipeline 2 is the first cooling liquid, the cooling liquid in the liquid changing tank 53 is the second cooling liquid, and when the first cooling liquid enters the liquid changing tank 53, the push plate 56 can prevent the first cooling liquid from directly flowing out, and can push out the corresponding second cooling liquid, thereby improving the liquid changing efficiency.
In this embodiment, be provided with on bin 1 and trade liquid and the corresponding material door mechanism 8 of getting of cooling subassembly 5, get material door mechanism 8 and include carousel 81, get material mouth 82, seal baffle 83 and articulated seat 84, wherein, carousel 81 rotates and sets up on bin 1, just the central axis of rotation of carousel 81 coincides with a plurality of positive reverse dynamo 6's array axis, seted up on carousel 81 and got material mouth 82, it is corresponding with the position that subassembly 7 was placed to reagent pipe to get material mouth 82, just the top of getting the material mouth is provided with seal baffle 83, one side of seal baffle 83 articulates on articulated seat 84, articulated seat 84 is fixed on bin 1.
In a preferred embodiment, a semiconductor cooler 511 and a temperature sensor are fixedly embedded in the liquid separation chamber 57.
In this embodiment, the system further comprises a controller, and the controller controls the semiconductor refrigerator 511, the temperature sensor forward and reverse rotation motor 6, the micro suction pump 52 and the circulation pump 4 in a unified manner.
In specific implementation, a plurality of reagent tubes are placed in the packaging bag 73, the temperature of the cooling liquid in the liquid changing and cooling assembly 5 is monitored through the temperature sensor, when only one group of the cooling liquid in the liquid changing and cooling assembly 5 does not reach the standard in a certain period, the controller controls the semiconductor refrigerator 511 to control the temperature, when two or more groups of the cooling liquid in the liquid changing and cooling assembly 5 do not reach the standard, the controller controls the circulating pump 4 to circulate the cooling liquid to continuously provide the cooler cooling liquid, controls the corresponding micro-suction pump to work, changes the cooling liquid in the liquid changing and cooling assembly 5, and when the reagent tubes are taken, the sealing baffle 83 is lifted.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.

Claims (8)

1. A detect BRAF gene mutation detect reagent storage device which characterized in that: the device comprises a storage box (1), a circulating pipeline (2), a refrigerating bin (3), a circulating pump (4), a liquid changing and cooling component (5) and a reagent tube placing component (7), wherein the circulating pipeline (2) is fixedly arranged below the inside of the storage box (1), the circulating pipeline (2) comprises an upper horizontal pipeline and a lower horizontal pipeline, the refrigerating bin (3) is embedded and communicated in the lower horizontal pipeline, and cooling liquid inside the circulating pipeline (2) is driven by the circulating pump (4) to flow in a circulating manner;
at least two groups of liquid changing and cooling assemblies (5) are sequentially communicated with the upper horizontal pipeline along the flowing direction of the cooling liquid, the liquid changing and cooling assemblies (5) can provide the cooling liquid with proper temperature for the reagent tube placing assembly (7), and the reagent tube placing assembly (7) is used for placing the reagent tube;
the liquid changing and cooling assembly (5) comprises a liquid inlet pipe (51), a micro suction pump (52), a liquid changing groove (53), a liquid outlet pipe (54) and a liquid dividing bin (57), wherein one end of the liquid inlet pipe (51) is connected into an upper horizontal pipeline, the other end of the liquid inlet pipe is communicated into the liquid changing groove (53), one end, far away from the liquid inlet pipe (51), of the liquid changing groove (53) is communicated with the liquid outlet pipe (54), the other end of the liquid outlet pipe (54) is communicated into the upper horizontal pipeline, the micro suction pump (52) capable of sucking cooling liquid into the liquid changing groove (53) from the upper horizontal pipeline is installed in the liquid inlet pipe (51), the liquid dividing bin (57) is fixedly communicated above the liquid changing groove (53), and the liquid dividing bin (57) can provide cooling liquid for the reagent pipe placing assembly (7);
a one-way valve mechanism (55) is arranged in the liquid outlet pipe (54), the one-way valve mechanism (55) comprises a liquid outlet bin (551), a liquid outlet (552) and a liquid blocking ball (554), wherein a conical liquid outlet (552) is arranged at one side of the liquid outlet bin (551), the outside of the liquid outlet (552) is connected with the liquid outlet pipe (54), the liquid blocking ball (554) is placed in the liquid outlet, the liquid blocking ball (554) is fixed at the end part of a piston rod (556), the other end of the piston rod (556) is arranged in a cylinder body (555) in a sealing and sliding manner, the cylinder body (555) is fixed at one end of the liquid outlet bin (556) far away from the liquid outlet (552), one end of the piston rod (556) far away from the liquid outlet (552) is connected with the cylinder body by adopting a return spring (557), and a plurality of through holes (551) are further distributed at one side of the liquid outlet bin (551) far away from the liquid outlet (552), the through hole (553) is coated by the liquid outlet pipe (54);
the elasticity of the return spring (557) can ensure that the reagent tube placing assembly (7) is filled with cooling liquid and the liquid blocking ball (554) is driven to be blocked at the liquid outlet (552) after the micro suction pump (52) stops working.
2. The storage device for the reagent for detecting BRAF gene mutation of claim 1, wherein: divide the inside in liquid storehouse (51) to be fixed with filter disc (58), filter disc (58) are the cavity structure, the outside fixed cover of filter disc (58) is equipped with annular fender stopper (59), the array has a plurality of pipes (510) of keeping in on the outer circumference of fender stopper (59), the one end of pipe (510) of keeping in is for sealing the form, and the other end passes and keeps off stopper (59) and stretch into in filter disc (58).
3. The storage device for the reagent for detecting BRAF gene mutation of claim 1, wherein: a plurality of positive and negative rotating motors (6) are arrayed on the outer circumference of the liquid separating bin (57), and a reagent tube placing assembly (7) is fixed at the output end of each positive and negative rotating motor (6).
4. The storage device for the reagent for detecting BRAF gene mutation of claim 3, wherein: the reagent tube placing assembly (7) comprises a liquid distribution disc (71), placing tubes (72) and a packaging bag (73), wherein the placing tubes (72) are fixedly embedded into the liquid distribution disc (71) in a circumferential array mode, the inner side wall and the bottom wall of each placing tube (72) are adhered with a tubular packaging bag (73), each packaging bag (73) is communicated with the liquid distribution disc (71) through a tube body, so that the packaging bags (73) are filled with cooling liquid, and the liquid distribution disc (71) is communicated with a temporary storage tube (510) through an elastic corrugated tube.
5. The storage device for the reagent for detecting BRAF gene mutation of claim 1, wherein: a push plate (56) is arranged in the liquid changing groove (53) in a sliding mode, the push plate (56) is connected with the side wall of the liquid changing groove (53) through a spring, and a space exists between the top of the push plate (56) and the top of the liquid changing groove (53).
6. The storage device for the reagent for detecting BRAF gene mutation of claim 1, wherein: be provided with on bin (1) and trade liquid and the corresponding material taking door mechanism (8) of cooling subassembly (5), material taking door mechanism (8) includes carousel (81), gets material mouth (82), seal baffle (83) and articulated seat (84), wherein, carousel (81) rotate and set up on bin (1), just the rotation central axis of carousel (81) coincides with the array axis of a plurality of just reversing motor (6), seted up on carousel (81) and got material mouth (82), it is corresponding with the position that subassembly (7) were placed to reagent pipe to get material mouth (82), just the top of getting the material mouth is provided with seal baffle (83), one side of seal baffle (83) articulates on articulated seat (84), articulated seat (84) are fixed on bin (1).
7. The storage device for the reagent for detecting BRAF gene mutation of claim 1, wherein: a semiconductor refrigerator (511) and a temperature sensor are fixedly embedded in the liquid distribution bin (57).
8. The storage device for the reagent for detecting BRAF gene mutation of claim 7, wherein: the device also comprises a controller which uniformly controls the semiconductor refrigerator (511), the temperature sensor positive and negative rotation motor (6), the micro suction pump (52) and the circulating pump (4).
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