CN112891305B - 利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 - Google Patents
利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 Download PDFInfo
- Publication number
- CN112891305B CN112891305B CN202110122194.2A CN202110122194A CN112891305B CN 112891305 B CN112891305 B CN 112891305B CN 202110122194 A CN202110122194 A CN 202110122194A CN 112891305 B CN112891305 B CN 112891305B
- Authority
- CN
- China
- Prior art keywords
- resveratrol
- unsaturated
- nanoemulsion
- guluronic acid
- loaded
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 title claims abstract description 90
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 title claims abstract description 90
- 235000021283 resveratrol Nutrition 0.000 title claims abstract description 90
- 229940016667 resveratrol Drugs 0.000 title claims abstract description 90
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 69
- AEMOLEFTQBMNLQ-BZINKQHNSA-N D-Guluronic Acid Chemical class OC1O[C@H](C(O)=O)[C@H](O)[C@@H](O)[C@H]1O AEMOLEFTQBMNLQ-BZINKQHNSA-N 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000003921 oil Substances 0.000 claims abstract description 21
- 239000004094 surface-active agent Substances 0.000 claims abstract description 17
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 16
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000008157 edible vegetable oil Substances 0.000 claims abstract description 5
- 235000019198 oils Nutrition 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 18
- 239000003814 drug Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 235000008390 olive oil Nutrition 0.000 claims description 5
- 239000004006 olive oil Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 239000003963 antioxidant agent Substances 0.000 abstract description 13
- 235000006708 antioxidants Nutrition 0.000 abstract description 13
- 239000000243 solution Substances 0.000 abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
- 238000000265 homogenisation Methods 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 3
- 238000005538 encapsulation Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 235000010443 alginic acid Nutrition 0.000 description 3
- 229920000615 alginic acid Polymers 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 229940072056 alginate Drugs 0.000 description 2
- -1 alginate oligosaccharide Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000000748 cardiovascular system Anatomy 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000043136 MAP kinase family Human genes 0.000 description 1
- 108091054455 MAP kinase family Proteins 0.000 description 1
- 102000003945 NF-kappa B Human genes 0.000 description 1
- 108010057466 NF-kappa B Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000004064 cosurfactant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000020095 red wine Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
- A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/062—Oil-in-water emulsions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/26—Optical properties
- A61K2800/262—Transparent; Translucent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Birds (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了一种利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法，涉及化学制剂技术领域。该制备方法包括步骤：S1、取5～10mg白藜芦醇溶解于0.2mL无水乙醇，再加入4.8mL的食用油中，混合均匀后过滤，获得油相；S2、将表面活性剂和油相混合均匀，得到混合物；取1.5mL混合物滴加入4～5mL的不饱和古罗糖醛酸水溶液中，经高速均质后，获得利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳。本发明的制备方法获得的利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳具有与水任意比例互溶、包封率高、稳定性高等优点，可在4℃放置45天不分层。此外，与相同浓度的白藜芦醇溶液相比，利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳具有更高的抗氧化能力。
Description
技术领域
本发明涉及化学制剂技术领域，尤其涉及一种利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法。
背景技术
纳米乳是由水相、油相、表面活性剂和助表面活性剂按一定比例自发形成，粒径为1～100nm的热力学稳定、透明或半透明的分散体系。纳米乳作为一种新型的药物传递系统，既可以增加难溶药物和脂溶性药物的溶解度，也可以负载水溶性药物，延长作用时间，提高药物的生物利用度，发挥缓释、靶向作用，因此纳米乳作为载体被大量应于食品与生物医药等行业。
白藜芦醇(Resveratrol，RES)作为一种天然多酚类物质，主要存在于葡萄、红酒、花生等植物中，具有多种生物活性。研究表明，白藜芦醇具有抗氧化能力，能够延缓衰老；有效为心血管减压，改善心肌缺血、抑制动脉粥样硬化，还能对抗血栓的形成，保护心血管；同时，对免疫系统也具有调节作用。但由于白藜芦醇存在水溶性差、见光易分解、口服吸收性差、生物利用率低、难以持久作用等问题，极大的限制了它在食品及化妆品领域的应用。如何提高白藜芦醇的生物利用率是本领域技术人员亟需解决的问题。
发明内容
本发明所要解决的技术问题是白藜芦醇的水溶性差、不稳定，如何利用O/W(水包油)型纳米乳载体来保护白藜芦醇生物活性的同时提高其抗氧化能力。
为了解决上述问题，本发明提出以下技术方案：
本发明提供一种利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳(以下简称：O/W型白藜芦醇纳米乳)的制备方法，包括以下步骤：
S1、取5～10mg白藜芦醇溶解于0.2mL无水乙醇，再加入4.8mL的食用油中，混合均匀后过滤，获得油相；
S2、将表面活性剂和油相混合均匀，得到混合物；取1.5mL混合物滴加入4～5mL的不饱和古罗糖醛酸水溶液中，经高速均质后，获得O/W型白藜芦醇纳米乳。
本发明制得的O/W型白藜芦醇纳米乳为澄清透明状。
可以理解地，所述S1、S2步骤中可使用磁力搅拌器辅助混合均匀。
需要说明的是，褐藻胶寡糖具有抗氧化、抗肿瘤、免疫调节和神经保护等作用。本发明涉及的不饱和古罗糖醛酸(GOS)是一种褐藻胶寡糖，是由褐藻胶分级后得到均聚古罗糖醛酸(PG)，PG再进一步酶解得到的。不饱和古罗糖醛酸能够调节巨噬细胞和神经小胶质细胞免疫紊乱，抑制脂多糖诱导的NF-κB和MAPK信号通路的过度激活及下游炎症介质过度分泌，具有良好的免疫调节作用。同时，不饱和古罗糖醛酸具有很好的抗氧化活性。申请人发现，将其作为O/W型白藜芦醇纳米乳的水相可以起到很好的抗氧剂的作用，还能增加白藜芦醇的抗氧化能力。
其进一步地技术方案为，所述不饱和古罗糖醛酸水溶液的浓度为1～5mg/mL。
其进一步地技术方案为，所述表面活性剂为Tween-80。
其进一步地技术方案为，所述食用油为橄榄油。
其进一步地技术方案为，所述步骤S2中，表面活性剂和油相的质量比为6-9：1-4。
其进一步地技术方案为，所述步骤S2中，均质速度为10000～13000rpm。
其进一步地技术方案为，所述步骤S2中，均质时间为1～2min
本发明还提供一种O/W型白藜芦醇纳米乳，由上述的O/W型白藜芦醇纳米乳制备方法制得。
本发明提供的O/W型白藜芦醇纳米乳，平均粒径为11.20±1.02nm。
本发明提供的O/W型白藜芦醇纳米乳在制备具有抗氧化作用的食品、药品、保健品、化妆品中的应用。
按上述方法制备得到的白藜芦醇纳米乳利用纳米激光粒度分析仪对其粒径、分散指数(PDI)和包封率进行了分析，并利用透射电镜对不饱和古罗糖醛酸纳米乳的表面形态进行了研究，发现本发明制得的白藜芦醇纳米乳呈现均一的纳米分散体系。
体外稳定性实验表明，在4℃条件下，白藜芦醇纳米乳在45天内稳定储存，没有出现分层现象。
利用超氧阴离子清除能力法比较白藜芦醇和白藜芦醇纳米乳的抗氧化能力，结果发现在同一浓度下，白藜芦醇纳米乳的抗氧化能力显著增强。
本发明的有益技术效果是：
1.本发明首次提供了一种O/W型白藜芦醇纳米乳的制备方法，该制备方法与高压法相比操作简单、能耗低，得到的白藜芦醇纳米乳可以有效地保护白藜芦醇的抗氧化等生物活性。
2.通过优化表面活性剂和油相的比例、是否均质的条件，获得的白藜芦醇纳米乳大小均一，平均粒径为11.20±1.02nm，包封率高达93％，并且通过稳定性实验表明本发明制得的白藜芦醇纳米乳在4℃条件下可稳定储存45天，粒径大小无明显变化。
3.通过比较本发明制得的O/W型白藜芦醇纳米乳与同浓度的白藜芦醇相比，包埋后的白藜芦醇的抗氧化能力显著增加。
附图说明
图1为本发明实施例1制得的白藜芦醇纳米乳的平均粒径图；
图2为本发明实施例2制得的白藜芦醇纳米乳的粒径分布图；
图3为本发明实施例2制得的白藜芦醇纳米乳的粒径分布图和透射电镜图；
图4为本发明实施例2、对比例1制得的白藜芦醇纳米乳的储存稳定性实验结果；
图5为本发明实施例2、对比例1制得的白藜芦醇纳米乳的抗氧化能力对比图。
具体实施方式
下面将结合本发明实施例中的附图，对实施例中的技术方案进行清楚、完整地描述。显然，以下将描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
应当理解，在此本发明实施例说明书中所使用的术语仅仅是出于描述特定实施例的目的而并不意在限制本发明实施例。如在本发明实施例说明书和所附权利要求书中所使用的那样，除非上下文清楚地指明其它情况，否则单数形式的“一”、“一个”及“该”意在包括复数形式。实验中未详述的试验操作均为本领域技术人员所熟知的常规试验操作。
实施例1表面活性剂和油相的比例对白藜芦醇纳米乳的影响
10mg白藜芦醇溶解于0.2mL无水乙醇后，将其加入4.8mL的橄榄油中，经磁力搅拌器混合均匀后，用0.8μm的滤膜过滤即可获得油相。将表面活性剂和油相按质量比6-9：1-4混合均匀后，边搅拌边缓慢滴加5mL浓度为5mg/mL的不饱和古罗糖醛酸水溶液，经高速分散器均质(11000rpm，1.5min)即可获得白藜芦醇纳米乳。
其中，只有表面活性剂和油相按质量比9：1获得的白藜芦醇纳米乳澄清透明，其余实施例均出现不同程度的分层现象，粒径大小结果见附图1。
实施例2均质对白藜芦醇纳米乳的影响
10mg白藜芦醇溶解于0.2mL无水乙醇后，将其加入4.8mL的橄榄油中，经磁力搅拌器混合均匀后，用0.8μm的滤膜过滤即可获得油相。将表面活性剂和油相按质量比9：1混合均匀后，边搅拌边缓慢滴加5mL浓度为5mg/mL的不饱和古罗糖醛酸水溶液，将样品一分为二，一份经高速分散器均质(11000rpm，1.5min)，一份不经过均质。二者均获得澄清透明的白藜芦醇纳米乳，但明显经过高速均质的粒径大小更为均一，其粒径大小分布结果见附图2。
对比例1水相对白藜芦醇纳米乳的影响
10mg白藜芦醇溶解于0.2mL无水乙醇后，将其加入4.8mL的橄榄油中，经磁力搅拌器混合均匀后，用0.8μm的滤膜过滤即可获得油相。将表面活性剂和油相按质量比9：1混合均匀后，边搅拌边缓慢滴加5mL纯水，将样品经高速分散器均质(11000rpm，1.5min)。获得澄清透明的白藜芦醇纳米乳。
实施例3白藜芦醇纳米乳的粒径检测及电镜观察
将实施例2中按表面活性剂和油相质量比为9：1获得的白藜芦醇纳米乳用超纯水稀释4倍后，测其粒径大小，见附图3右。同时将稀释后的样品滴在覆有支持膜的铜网上，自然挥干，用透射电子显微镜观察纳米乳形态，见附图3右。图中近似圆形的黑点即为白藜芦醇纳米乳液滴，可见本发明实施例制得的白藜芦醇纳米乳大小均一。
实施例4白藜芦醇纳米乳的稳定性实验
将实施例2、对比例1中按表面活性剂和油相质量比为9：1获得的白藜芦醇纳米乳置于西林瓶中，放置冰箱4℃保存，在第0，15，30，45，60，90天取样观察并测定其粒径，评价其稳定性。
结果见附图4，表明水/不饱和古罗糖醛酸水溶液包埋白藜芦醇获得的纳米乳在冰箱4℃保存45天粒径变化都很小，且无分层现象，可在4℃保存。
实施例5白藜芦醇纳米乳的抗氧化能力检测
利用超氧阴离子清除能力试剂盒检测实施例2、对比例1、白藜芦醇溶液、不饱和古罗糖醛酸水溶液的抗氧化性，结果见附图5。从图中看出，与同浓度的白藜芦醇溶液相比，纯水包埋白藜芦醇获得的纳米乳抗氧化性明显偏低；不饱和古罗糖醛酸水溶液包埋白藜芦醇获得的纳米乳的抗氧化能力高于白藜芦醇溶液以及不饱和古罗糖醛酸水溶液，说明用不饱和古罗糖醛酸水溶液包埋白藜芦醇获得的纳米乳可以增加白藜芦醇的抗氧化能力，这是由于不饱和古罗糖醛酸具有的抗氧化能力与纳米乳的包埋方式共同作用实现的，本发明制得的O/W型白藜芦醇纳米乳在各步骤和各组分具有协同增效的作用。
小结：
通过筛选表面活性剂和油相的比例、是否均质的条件，获得的白藜芦醇纳米乳大小均一，平均粒径为11.20±1.02nm，包封率高达93％，并且通过稳定性实验表明本发明制得的白藜芦醇纳米乳在4℃条件下可稳定储存45天，粒径大小无明显变化。
通过比较本发明制得的O/W型白藜芦醇纳米乳与同浓度的白藜芦醇相比，其抗氧化能力显著增加，有利于进一步发挥白藜芦醇的活性作用，拓展应用范围。
在上述实施例中，对各个实施例的描述都各有侧重，某个实施例中没有详细描述的部分，可以参见其他实施例的相关描述。
以上所述，为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，可轻易想到各种等效的修改或替换，这些修改或替换都应涵盖在本发明的保护范围之内。因此，本发明的保护范围应以权利要求的保护范围为准。
Claims (5)
1.一种利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法，其特征在于，包括以下步骤：
S1、取5～10mg白藜芦醇溶解于0.2mL无水乙醇，再加入4.8mL的食用油中，混合均匀后过滤，获得油相；
S2、将表面活性剂和油相混合均匀，得到混合物；取1.5mL混合物滴加入4～5mL的不饱和古罗糖醛酸水溶液中，经高速均质后，获得O/W型白藜芦醇纳米乳，平均粒径为11.20±1.02nm；
所述不饱和古罗糖醛酸水溶液的浓度为1～5mg/mL；
所述步骤S2中，表面活性剂和油相的质量比为6-9：1-4；
所述步骤S2中，均质速度为10000～13000rpm；
所述步骤S2中，均质时间为1～2min。
2.如权利要求1所述的利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法，其特征在于，所述表面活性剂为Tween-80。
3.如权利要求1所述的利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法，其特征在于，所述食用油为橄榄油。
4.一种利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳，其特征在于，由权利要求1-3任意一项所述的利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳的制备方法制得。
5.如权利要求4所述的利用不饱和古罗糖醛酸负载白藜芦醇的O/W型纳米乳在制备具有抗氧化作用的食品、药品、保健品、化妆品中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110122194.2A CN112891305B (zh) | 2021-01-27 | 2021-01-27 | 利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110122194.2A CN112891305B (zh) | 2021-01-27 | 2021-01-27 | 利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112891305A CN112891305A (zh) | 2021-06-04 |
| CN112891305B true CN112891305B (zh) | 2022-03-08 |
Family
ID=76120699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110122194.2A Active CN112891305B (zh) | 2021-01-27 | 2021-01-27 | 利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112891305B (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113180241B (zh) * | 2021-04-16 | 2022-12-27 | 江南大学 | 一种用于高效包封白藜芦醇的乳剂及其应用 |
| CN114010512B (zh) * | 2021-10-29 | 2022-10-21 | 宝萃生物科技有限公司 | 一种复合纳米乳液及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214225A (zh) * | 2007-12-29 | 2008-07-09 | 西北农林科技大学 | 一种白藜芦醇纳米乳抗癌药物 |
| US9872832B2 (en) * | 2015-10-23 | 2018-01-23 | LG Bionano, LLC | Nanoemulsions having reversible continuous and dispersed phases |
| CN111643452A (zh) * | 2020-05-22 | 2020-09-11 | 华南农业大学 | 一种氧化白藜芦醇纳米乳液及其制备方法与应用 |
| CN111658613B (zh) * | 2020-07-13 | 2021-02-09 | 深圳大学 | 一种w/o/w型不饱和古罗糖醛酸纳米乳及其制备方法 |
-
2021
- 2021-01-27 CN CN202110122194.2A patent/CN112891305B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112891305A (zh) | 2021-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zambrano-Zaragoza et al. | The effect of nano-coatings with α-tocopherol and xanthan gum on shelf-life and browning index of fresh-cut “Red Delicious” apples | |
| Feng et al. | Preparation and characterization of emulsion-filled gel beads for the encapsulation and protection of resveratrol and α-tocopherol | |
| CN112891305B (zh) | 利用不饱和古罗糖醛酸负载白藜芦醇的o/w型纳米乳的制备方法 | |
| Chi et al. | Preparation of nanoliposomal carriers to improve the stability of anthocyanins | |
| Wang et al. | Formulation of water-in-oil-in-water (W/O/W) emulsions containing trans-resveratrol | |
| Talebi et al. | Effects of different stabilizers on colloidal properties and encapsulation efficiency of vitamin D3 loaded nano-niosomes | |
| Tubtimsri et al. | Fabrication and characterization of spearmint oil loaded nanoemulsions as cytotoxic agents against oral cancer cell | |
| CN102552058B (zh) | 复合维生素脂质纳米粒及其制备方法 | |
| Li et al. | In vitro and in vivo evaluation of a simple microemulsion formulation for propofol | |
| CN103800304A (zh) | 用于包封亲水性和/或亲油性分子的组合物 | |
| CN1843199A (zh) | 纳米级β－胡萝卜素乳状液及其制备方法和用途 | |
| Lacatusu et al. | Lipid nanocarriers based on natural compounds: An evolving role in plant extract delivery | |
| Chen et al. | Self-nanoemulsions loaded with dihydromyricetin: Insights to their formulation stability | |
| Moradi et al. | Preparation and characterization of α-tocopherol nanocapsules based on gum Arabic-stabilized nanoemulsions | |
| Niu et al. | Co-encapsulation of chlorogenic acid and cinnamaldehyde essential oil in Pickering emulsion stablized by chitosan nanoparticles | |
| KR101990060B1 (ko) | 레스베라트롤의 나노캡슐 제조방법 및 제조된 레스베라트롤 나노캡슐 | |
| Miranda-Linares et al. | Spray-drying method for the encapsulation of a functionalized ingredient in alginate-pectin nano-and microparticles loaded with distinct natural actives: Stability and antioxidant effect | |
| Rahman et al. | Development of self-nanoemulsifying tablet (SNET) for bioavailability enhancement of sertraline | |
| Hyma et al. | Formulation and characterisation of self-microemulsifying drug delivery system of pioglitazone | |
| Rosli et al. | Formulation and characterization of nanostructured lipid carrier encapsulated Zingiber zerumbet oil using ultrasonication technique | |
| Calderón-Santoyo et al. | Microencapsulation of citral and its antifungal activity into pectin films | |
| CN112544982B (zh) | 一种纳米硒皮克林乳液及其制备方法与应用 | |
| CN108309936B (zh) | 载番茄红素自组装纳米胶束及其制备方法 | |
| CN114948880B (zh) | 一种咖啡酸苯乙酯纳米稳定缓释剂型的制备方法 | |
| Kumar et al. | Effect of self nanoemulsifying drug delivery system (SNEDDS) on intestinal permeation and anti-diabetic activity of Berberis aristata extract: in-vitro and ex-vivo studies |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210604 Assignee: Suzhou Nicholas Intelligent Equipment Co.,Ltd. Assignor: SHENZHEN University Contract record no.: X2022980024373 Denomination of invention: Preparation method of O/W nano emulsion loaded with resveratrol by unsaturated guluronic acid Granted publication date: 20220308 License type: Common License Record date: 20221201 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210604 Assignee: Shenzhen Nonnegative Nianhua Technology Co.,Ltd. Assignor: SHENZHEN University Contract record no.: X2022980026216 Denomination of invention: Preparation method of O/W nano emulsion loaded with resveratrol by unsaturated guluronic acid Granted publication date: 20220308 License type: Common License Record date: 20221211 Application publication date: 20210604 Assignee: Shenzhen Dongfang Renshou Life Technology Co.,Ltd. Assignor: SHENZHEN University Contract record no.: X2022980025926 Denomination of invention: Preparation method of O/W nano emulsion loaded with resveratrol by unsaturated guluronic acid Granted publication date: 20220308 License type: Common License Record date: 20221211 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210604 Assignee: Guangdong Tianzhuang Cosmetics Co.,Ltd. Assignor: SHENZHEN University Contract record no.: X2023980030950 Denomination of invention: Preparation of O/W type nanoemulsion using unsaturated glucuronic acid loaded with resveratrol Granted publication date: 20220308 License type: Common License Record date: 20230116 |
|
| EE01 | Entry into force of recordation of patent licensing contract |