CN112877210A - Stem cell culture perfusion device with controllable pH value - Google Patents
Stem cell culture perfusion device with controllable pH value Download PDFInfo
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- CN112877210A CN112877210A CN202110210565.2A CN202110210565A CN112877210A CN 112877210 A CN112877210 A CN 112877210A CN 202110210565 A CN202110210565 A CN 202110210565A CN 112877210 A CN112877210 A CN 112877210A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
Abstract
The invention provides a stem cell culture perfusion device with a controllable pH value, relates to the technical field of stem cell culture perfusion, and solves the problems that after stem cells are injected into the conventional stem cell culture perfusion device, in order to accelerate adaptation of the stem cells after cold melting, improve cell culture efficiency, a stirring mechanism is mostly adopted for uniformly mixing and stirring, but due to the arrangement of the stirring mechanism, the stem cells are easily damaged by direct contact of the stirring mechanism, and defects exist. The utility model provides a controllable stem cell of pH value cultivates perfusion device, includes cylindric casing, the inside fixed mounting of cylindric casing has cylindric main part, and sliding connection has the pole that rises in cylindric main part. After the stem cells are added into the perfusion cavity from the perfusion input hole part, the stem cells firstly contact with the flow baffle and sequentially slide along the two rows of flow baffles to flow downwards under the self weight, and through the arrangement of the two rows of flow baffles, the uniform mixing of the added stem cells is accelerated, so that the stem cells adapt as soon as possible after being cooled and melted, and the cell culture efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of stem cell culture perfusion, and particularly relates to a stem cell culture perfusion device with a controllable pH value.
Background
The stem cells are multipotential cells with self-replication ability, can be differentiated into various functional cells under certain conditions, are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are divided into three categories according to the development potentiality of the stem cells; the stem cell is an insufficiently differentiated and immature cell, has the potential function of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field.
For example, application No.: CN201811097231.3 the invention provides a stem cell culture perfusion device, which comprises a perfusion tube, a perfusion bottle, a perfusion mechanism, a blowout prevention tube, a cleaning mechanism, a tube joint, a sleeve head, a vent pipe, a drain pipe, a sleeve, an oil pipe, an air injection joint, a connector, a spray head, a continuous pipe channel and an inner annular channel; the stem cell culture perfusion device is strong in practicability, simple to operate, good in safety, high in cleanliness, convenient to use and high in reliability.
Based on the search of the above patents and the discovery of combining with the devices in the prior art, after stem cells are injected into the conventional stem cell culture perfusion device, in order to accelerate adaptation of the stem cells after cold melting, and improve the cell culture efficiency, a stirring mechanism is mostly adopted to perform uniform mixing and stirring so as to accelerate adaptation of the stem cells after cold melting, but due to the arrangement of the stirring mechanism, damage to the stem cells is easily caused by direct contact of the stem cells, and defects exist.
Disclosure of Invention
In order to solve the technical problems, the invention provides a stem cell culture perfusion device with a controllable pH value, which aims to solve the problems that after stem cells are injected into the conventional stem cell culture perfusion device, in order to accelerate adaptation of the conventional stem cell culture perfusion device as soon as possible after cold melting of the stem cells and improve cell culture efficiency, a stirring mechanism is mostly adopted for uniformly mixing and stirring so as to accelerate adaptation of the conventional stem cell culture perfusion device as soon as possible after cold melting of the stem cells, but due to the arrangement of the stirring mechanism, damage to the stem cells is easily caused by direct contact of the stirring mechanism, and defects are insufficient.
The purpose and the effect of the stem cell culture perfusion device with the controllable pH value are achieved by the following specific technical means:
a stem cell culture perfusion device with a controllable pH value comprises a cylindrical shell, wherein a cylindrical main body is fixedly arranged in the cylindrical shell, and a lifting pull rod is connected in the cylindrical main body in a sliding mode; the cylindrical main body comprises a gas output through hole and a food grade pipe, the bottom end of the circular base plate is bilaterally symmetrical and is provided with a row of gas output through holes communicated with the annular gas input cavity, the food grade pipe is arranged on the bottom end face of the circular base plate relative to the gas output through hole, the cylindrical main body is fixedly inserted in the axis position of the cylindrical shell, the circular top plate is positioned at the top of the cylindrical main body, the circular base plate is positioned in the perfusion cavity, the bottom end of the food grade pipe is an inclined plane, and the inclined plane is parallel to the inclined flow slope and is not in contact with the.
Furthermore, the cylindrical shell comprises an perfusion cavity, an oblique flow slope and perfusion output holes, the perfusion cavity is formed in the cylindrical shell, the oblique flow slope is symmetrically arranged on the left side and the right side of the bottom surface of the inner end of the perfusion cavity, an included angle of twenty-five degrees is formed between the oblique flow slope and the bottom surface of the inner end of the cylindrical shell, and the perfusion output holes are formed in the center of the bottom surface of the inner end of the perfusion cavity.
Furthermore, the cylindrical shell comprises perfusion partition plates and perfusion input holes, the inside of the perfusion cavity is bilaterally symmetrical and is provided with two perfusion partition plates, the top end faces of the two perfusion partition plates are fixedly connected with the top end face of the inner end of the perfusion cavity, the bottom end faces of the two perfusion partition plates are not in contact with the inclined flow slope inclined planes at opposite positions, and the top end face of the inner end of the perfusion cavity is provided with a perfusion input hole penetrating through the top end face of the cylindrical shell relative to the space between the two perfusion partition plates and the cavity on the circumferential surface of the inner cavity of the perfusion cavity.
Furthermore, the cylindrical shell comprises flow baffles, the end faces, facing the inner peripheral surface of the perfusion cavity, of the two perfusion baffles are uniformly distributed from top to bottom, and are provided with a row of flow baffles, the inner peripheral surface, opposite to the two perfusion baffles, of the perfusion cavity is also uniformly distributed from top to bottom, and the two rows of flow baffles are staggered from top to bottom, and are of an inclined downward structure.
Further, cylindric main part is including circular top dish, annular gas chamber, gas input port, gas input through-hole and cylindric purification chamber, cylindric main part top end face fixedly connected with one rather than the circular top dish of coaxial axle center, circular top dish is inside to be seted up one rather than the annular gas chamber of coaxial axle center, circular top dish outer peripheral face is provided with the gas input port who is linked together with annular gas chamber, cylindric purification chamber has been seted up to cylindric main part inside one side, and cylindric purification chamber top end face axle center position is linked together with annular gas chamber through a gas input through-hole.
Further, cylindric main part is including active carbon filter layer, circular chassis, cyclic annular gas input chamber and sleeve pipe, cylindric main part bottom end face fixedly connected with rather than the circular chassis of coaxial axle center, circular chassis is inside to be seted up rather than the cyclic annular gas input chamber of coaxial axle center, cylindric purification chamber bottom end face axle center position is linked together with cyclic annular gas input chamber through a gas input through-hole, and cylindric main part, circular top dish and circular chassis axle center position are fixed to be pegged graft and are had a sleeve pipe, are provided with the active carbon filter layer in the cylindric purification chamber.
Further, the lifting pull rod comprises a pull disc, a reset spring, a circular connecting block and a sealing plug, the axis position of the top end face of the lifting pull rod is fixedly connected with the pull disc, the bottom end face of the pull disc is fixedly connected with the reset spring, the reset spring is sleeved on the periphery of the lifting pull rod, the bottom end face of the lifting pull rod is fixedly connected with the sealing plug through the circular connecting block, the top end of the circular connecting block is of an arc structure, the lifting pull rod is slidably connected in the sleeve, the bottom end face of the reset spring is welded with the top end face of the circular top disc, and the sealing plug is in sealing contact with the opening end of the perfusion output hole in the.
Compared with the prior art, the invention has the following beneficial effects:
when stem cells are added, the stem cells are added into a perfusion cavity from a perfusion input hole part, two perfusion partition plates are symmetrically arranged in the perfusion cavity, the end faces of the two perfusion partition plates facing to the inner peripheral surface of the perfusion cavity are uniformly distributed up and down, a row of flow baffle plates are arranged on the inner peripheral surface of the perfusion cavity opposite to the two perfusion partition plates, and the two row of flow baffle plates are also uniformly distributed up and down on the inner peripheral surface of the perfusion cavity opposite to the two perfusion partition plates and are staggered up and down, and the flow baffle plates are of an inclined downward structure, so that after the stem cells are added into the perfusion cavity from the perfusion input hole part, the stem cells firstly contact the flow baffle plates and sequentially slide down along the two row of flow baffle plates under the self weight, the uniform mixing of the added stem cells is accelerated, the cold melting and adaptation of the stem cells are accelerated, and the cell culture efficiency is improved.
According to the invention, the bottom end face of the circular base plate is provided with the food grade pipe relative to the gas output through hole part, the bottom end of the food grade pipe is an inclined plane, and the inclined plane is parallel to but not in contact with the inclined flow slope of the inclined flow slope, so that after stem cells are added, the gas generation equipment is connected with the gas input port through a hose, the gas generated by the gas generation equipment is started to be input into the annular gas cavity through the gas input port, and is input into the annular gas input cavity through the gas input through hole and the cylindrical purification cavity, and then is output from the gas output through hole part, and the gas output from the gas output through hole part blows the stem cells in the perfusion cavity through the food grade pipe, so that the stem cells are uniformly mixed through the non-contact of the gas, the adaptation of the stem cells after cold; furthermore, an active carbon filter layer is arranged in the cylindrical purification cavity, and the gas entering the cylindrical purification cavity can be filtered through the active carbon filter layer, so that no gas impurities are input into the perfusion cavity; when other substances such as growth factors, nutrients and the like need to be supplemented, the growth factors can be added into the perfusion cavity through the other perfusion input hole, the flowing mode of the perfusion cavity is consistent with that of the perfusion cavity after stem cells are injected, and the PH value can be controlled by adding the other substances such as the growth factors, the nutrients and the like.
Drawings
Fig. 1 is a schematic sectional structure of the present invention.
Fig. 2 is a schematic view of the present invention at a part enlarged in fig. 1.
Fig. 3 is a schematic cross-sectional view of the cylindrical housing of the present invention.
Fig. 4 is a cross-sectional structural view of a cylindrical body of the present invention.
Fig. 5 is a cross-sectional structural view of the circular chassis of the present invention.
FIG. 6 is a schematic view of the cylindrical body of FIG. 1 shown in an isolated configuration.
In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:
1. a cylindrical housing; 101. a perfusion cavity; 102. an inclined flow slope; 103. a perfusion output hole; 104. a perfusion baffle plate; 105. a baffle plate; 106. a perfusion input aperture; 2. a cylindrical body; 201. a circular top plate; 202. an annular gas chamber; 203. a gas input port; 204. a gas input through hole; 205. an activated carbon filter layer; 206. a circular chassis; 207. an annular gas input cavity; 208. a sleeve; 209. a gas output through hole; 2010. a food grade tube; 2011. a cylindrical purification chamber; 3. lifting the pull rod; 301. pulling the disc; 302. a return spring; 303. a circular connecting block; 304. and (4) sealing the plug.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example (b):
as shown in figures 1 to 6:
the invention provides a stem cell culture perfusion device with a controllable pH value, which comprises: the device comprises a cylindrical shell 1, wherein a cylindrical main body 2 is fixedly arranged in the cylindrical shell 1, and a lifting pull rod 3 is connected in the cylindrical main body 2 in a sliding manner; the cylindrical shell 1 comprises a perfusion cavity 101, an oblique flow slope 102 and a perfusion output hole 103, the perfusion cavity 101 is formed in the cylindrical shell 1, the oblique flow slope 102 is symmetrically arranged on the left side and the right side of the bottom surface of the inner end of the perfusion cavity 101, an included angle of twenty-five degrees is formed between the inclined plane of the oblique flow slope 102 and the bottom surface of the inner end of the cylindrical shell 1, and the perfusion output hole 103 is formed in the center of the bottom surface of the inner end of the perfusion cavity 101; the cylindrical shell 1 comprises perfusion partition plates 104 and perfusion input holes 106, the inside of the perfusion cavity 101 is provided with two perfusion partition plates 104 in bilateral symmetry, the top end faces of the two perfusion partition plates 104 are fixedly connected with the top faces of the inner ends of the perfusion cavity 101, the bottom end faces of the two perfusion partition plates 104 are not in contact with the inclined flow slopes 102 at opposite positions, and the top faces of the inner ends of the perfusion cavity 101, opposite to the two perfusion partition plates 104 and the inner circumferential cavity of the perfusion cavity 101, are provided with perfusion input holes 106 penetrating through the top end faces of the cylindrical shell 1; the cylindrical shell 1 comprises flow baffles 105, a row of flow baffles 105 are uniformly distributed from top to bottom on the end faces of the two perfusion clapboards 104 facing to the inner circumferential surface of the perfusion cavity 101, a row of flow baffles 105 are uniformly distributed from top to bottom on the inner circumferential surface of the perfusion cavity 101 opposite to the two perfusion clapboards 104, a row of flow baffles 105 are also uniformly distributed from top to bottom on the inner circumferential surface of the perfusion cavity 101, the two rows of flow baffles 105 are staggered from top to bottom, and the flow baffles 105 are inclined downward; the cylindrical main body 2 comprises a gas output through hole 209 and a food level pipe 2010, the bottom end surface of the circular base plate 206 is bilaterally symmetrical and is respectively provided with a row of gas output through holes 209 communicated with the annular gas input cavity 207, the food level pipe 2010 is arranged on the bottom end surface of the circular base plate 206 relative to the gas output through holes 209, the cylindrical main body 2 is fixedly inserted and connected at the axis part of the cylindrical shell 1, the circular top plate 201 is positioned at the top of the cylindrical main body 2, the circular base plate 206 is positioned in the perfusion cavity 101, the bottom end of the food level pipe 2010 is an inclined surface, and the inclined surface is parallel to the inclined surface of the inclined flow slope; cylindrical main part 2 is including circular top dish 201, annular gas chamber 202, gas input port 203, gas input through-hole 204 and cylindric purification chamber 2011, cylindrical main part 2 top end face fixedly connected with one rather than the circular top dish 201 of coaxial axle center, circular top dish 201 is inside to be offered one rather than the annular gas chamber 202 of coaxial axle center, circular top dish 201 outer peripheral face is provided with the gas input port 203 that is linked together with annular gas chamber 202, a cylindric purification chamber 2011 has been offered to inside one side of cylindrical main part 2, and cylindric purification chamber 2011 top end face axle center position is linked together with annular gas chamber 202 through a gas input through-hole 204.
The cylindrical main body 2 comprises an activated carbon filter layer 205, a circular base plate 206, an annular gas input cavity 207 and a sleeve 208, the bottom end face of the cylindrical main body 2 is fixedly connected with the circular base plate 206 which is coaxial with the cylindrical main body, the annular gas input cavity 207 which is coaxial with the circular base plate 206 is arranged in the circular base plate 206, the axis part of the bottom end face of the cylindrical purification cavity 2011 is communicated with the annular gas input cavity 207 through a gas input through hole 204, the sleeve 208 is fixedly inserted in the axis parts of the cylindrical main body 2, the circular top plate 201 and the circular base plate 206, and the activated carbon filter layer 205 is arranged in the cylindrical purification cavity 2011.
The lifting pull rod 3 comprises a pull disc 301, a reset spring 302, a circular connecting block 303 and a sealing plug 304, the axis position of the top end face of the lifting pull rod 3 is fixedly connected with the pull disc 301, the bottom end face of the pull disc 301 is fixedly connected with the reset spring 302, the reset spring 302 is sleeved on the periphery of the lifting pull rod 3, the bottom end face of the lifting pull rod 3 is fixedly connected with the sealing plug 304 through the circular connecting block 303, the top end of the circular connecting block 303 is of an arc structure, the lifting pull rod 3 is slidably connected in the sleeve 208, the bottom end face of the reset spring 302 is welded with the top end face of the circular top disc 201, and the sealing plug 304 is in sealing tangency with the opening end of the perfusion output hole 103.
When in use:
when stem cells are added, the stem cells are added into the perfusion cavity 101 from a perfusion input hole 106, because the perfusion cavity 101 is internally provided with two perfusion clapboards 104 which are bilaterally symmetrical, the end surfaces of the two perfusion clapboards 104 facing the inner circumferential surface of the perfusion cavity 101 are uniformly distributed up and down with a row of flow baffle plates 105, the inner circumferential surface of the perfusion cavity 101 opposite to the two perfusion clapboards 104 is also uniformly distributed up and down with a row of flow baffle plates 105, the two rows of flow baffle plates 105 are staggered up and down, the flow baffle plates 105 are in an inclined downward structure, after the stem cells are added into the perfusion cavity 101 from the perfusion input hole 106, the stem cells firstly contact the flow baffle plates 105 and sequentially slide down along the two rows of flow baffle plates 105 under the self weight, the uniform mixing of the added stem cells is accelerated through the arrangement of the two rows of flow baffle plates 105, the cold mixing of the stem cells is accelerated to adapt to the dry cells as soon as possible, the cell culture efficiency is improved, and the left side and the right side of the bottom surface of the inner end of the perfusion cavity 101 are symmetrically provided with the inclined flow slopes 102, so that the stem cells flowing down from the two rows of flow baffles 105 flow to the middle part of the perfusion cavity 101 along the inclined flow slopes 102;
according to the invention, the food-grade tube 2010 is arranged on the bottom end surface of the circular chassis 206 relative to the gas output through hole 209, the bottom end of the food-grade tube 2010 is an inclined surface, and the inclined surface is parallel to but not in contact with the inclined surface of the inclined flow slope 102, so that after stem cells are added, the gas generating equipment is connected with the gas input port 203 through a hose, the gas generating equipment starts the gas generated by the gas generating equipment to be input into the annular gas cavity 202 through the gas input port 203, and is input into the annular gas input cavity 207 through the gas input through hole 204 and the cylindrical purification cavity 2011, and then is output from the gas output through hole 209, and the gas output from the gas output through hole 209 stirs the stem cells in the perfusion cavity 101 through the food-grade tube 2010, so that the stem cells are uniformly mixed through non-contact gas, adaptation after the stem cells are cooled is accelerated, and the; further, an activated carbon filter layer 205 is arranged in the cylindrical purification cavity 2011, and the gas entering the cylindrical purification cavity 2011 can be filtered through the arrangement of the activated carbon filter layer 205, so that no gas impurities are input into the perfusion cavity 101;
when other substances such as growth factors and nutrients need to be supplemented, the growth factors can be added into the perfusion cavity 101 through the other perfusion input hole 106, the flowing mode of the growth factors is consistent with that of the perfusion cavity after stem cells are injected, and the control of the pH value can be realized by adding the other substances such as the growth factors and the nutrients;
when the uniformly mixed stem cells need to be discharged, the pull rod 3 can be pulled up along the sleeve 208 through the pull disc 301, the return spring 302 is stretched, the sealing plug 304 is separated from the perfusion output hole 103 at this time, the stem cells are discharged from the perfusion output hole 103 without the sealing of the sealing plug 304, after the stem cells are discharged, the pull disc 301 pulled up is only required to be loosened, and the sealing plug 304 and the opening end of the perfusion output hole 103 are sealed again under the resetting action of the return spring 302; furthermore, the top end surface of the circular connecting block 303 is in an arc structure, so that stem cells can flow down along the top end surface of the circular connecting block 303 through the design of the arc structure, and no dead angle residue is ensured.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (7)
1. A stem cell culture perfusion device with controllable pH value is characterized in that: the lifting device comprises a cylindrical shell (1)), a cylindrical main body (2) is fixedly arranged in the cylindrical shell (1), and a lifting rod (3) is connected in the cylindrical main body (2) in a sliding manner; cylindric main part (2) are including gas output through-hole (209) and food level pipe (2010), circular chassis (206) bottom is personally submitted the bilateral symmetry form and respectively sets up one row of gas output through-hole (209) that are linked together with cyclic annular gas input chamber (207), food level pipe (2010) are installed for gas output through-hole (209) position to circular chassis (206) bottom face, cylindric main part (2) are fixed to be pegged graft at cylindric casing (1) axle center position, circular top dish (201) are located cylindric main part (2) top, circular chassis (206) are located perfusion chamber (101), food level pipe (2010) bottom is the inclined plane, and this inclined plane and oblique flow slope (102) inclined plane parallel but contactless.
2. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: the cylindrical shell (1) comprises an perfusion cavity (101), oblique flow slopes (102) and perfusion output holes (103), the perfusion cavity (101) is formed in the cylindrical shell (1), the oblique flow slopes (102) are symmetrically arranged on the left side and the right side of the inner bottom surface of the perfusion cavity (101), an included angle of twenty-five degrees is formed between the inclined planes of the oblique flow slopes (102) and the inner bottom surface of the cylindrical shell (1), and the perfusion output holes (103) are formed in the center of the inner bottom surface of the perfusion cavity (101).
3. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: the cylindrical shell (1) comprises perfusion partition plates (104) and perfusion input holes (106), the perfusion cavity (101) is internally provided with two perfusion partition plates (104) in a bilateral symmetry mode, the top end faces of the two perfusion partition plates (104) are fixedly connected with the top faces of the inner ends of the perfusion cavity (101), the bottom end faces of the two perfusion partition plates (104) are not in contact with inclined planes of the inclined flow slopes (102) at opposite positions, and the perfusion input holes (106) penetrating through the top end faces of the cylindrical shell (1) are formed in the top faces of the inner ends of the perfusion cavity (101) relative to the space between the two perfusion partition plates (104) and the inner circumferential cavity of the perfusion cavity (101).
4. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: the cylindrical shell (1) comprises flow baffles (105), the end faces, facing the inner circumferential face of the perfusion cavity (101), of the two perfusion partition plates (104) are provided with a row of flow baffles (105) in an up-down uniform distribution mode, the inner circumferential face of the perfusion cavity (101) opposite to the two perfusion partition plates (104) is also provided with a row of flow baffles (105) in an up-down uniform distribution mode, the two rows of flow baffles (105) are staggered up and down, and the flow baffles (105) are of an inclined downward structure.
5. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: cylindric main part (2) are including circular head dish (201), annular gas chamber (202), gas input port (203), gas input through-hole (204) and cylindric purification chamber (2011), cylindric main part (2) top end face fixedly connected with one rather than circular head dish (201) of coaxial axle center, circular head dish (201) is inside to be seted up one and is located rather than annular gas chamber (202) of coaxial axle center, circular head dish (201) outer peripheral face is provided with gas input port (203) that are linked together with annular gas chamber (202), cylindric purification chamber (2011) has been seted up to cylindric main part (2) inside one side, and cylindric purification chamber (2011) top end face axle center position is linked together with annular gas chamber (202) through a gas input through-hole (204).
6. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: cylindric main part (2) are including activated carbon filter layer (205), circular chassis (206), annular gas input chamber (207) and sleeve pipe (208), cylindric main part (2) bottom end face fixedly connected with rather than circular chassis (206) with the axle center, circular chassis (206) inside set up rather than annular gas input chamber (207) with the axle center, cylindric purification chamber (2011) bottom end face axle center position is linked together with annular gas input chamber (207) through a gas input through-hole (204), cylindric main part (2), circular top dish (201) and circular chassis (206) axle center position are fixed to be pegged graft and are had a sleeve pipe (208), be provided with activated carbon filter layer (205) in cylindric purification chamber (2011).
7. The stem cell culture perfusion apparatus of claim 1, wherein the apparatus comprises: the lifting pull rod (3) comprises a pull disc (301), a reset spring (302), a circular connecting block (303) and a sealing plug (304), the top end face axis position of the lifting pull rod (3) is fixedly connected with the pull disc (301), the bottom end face of the pull disc (301) is fixedly connected with the reset spring (302), the reset spring (302) is sleeved on the periphery of the lifting pull rod (3), the bottom end face of the lifting pull rod (3) is fixedly connected with the sealing plug (304) through the circular connecting block (303), the top end of the circular connecting block (303) is of an arc structure, the lifting pull rod (3) is slidably connected into a sleeve (208), the bottom end face of the reset spring (302) is welded with the top end face of the circular top disc (201), and the sealing plug (304) is in a sealing tangent mode with the opening end of a perfusion output hole (103) under the ordinary state of the reset.
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| CN202110210565.2A CN112877210B (en) | 2021-02-25 | 2021-02-25 | Stem cell culture perfusion device with controllable pH value |
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| CN202110210565.2A CN112877210B (en) | 2021-02-25 | 2021-02-25 | Stem cell culture perfusion device with controllable pH value |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| WO2009034186A2 (en) * | 2007-09-13 | 2009-03-19 | Helmholtz-Zentrum für Infektionsforschung GmbH | Process for cell cultivation |
| WO2015176653A1 (en) * | 2014-05-22 | 2015-11-26 | 第三军医大学第三附属医院 | Bioreactor for three-dimensional tissue perfusion culture |
| CN209128459U (en) * | 2018-11-06 | 2019-07-19 | 天津赛尔康生物医药科技有限公司 | A kind of stem cell culture perfusing device |
| CN211546569U (en) * | 2020-01-06 | 2020-09-22 | 上海博威生物医药有限公司 | Perfusion device for stem cell culture |
| CN111778162A (en) * | 2020-07-29 | 2020-10-16 | 湖北明德健康科技有限公司 | Stem cell culture perfusion device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
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2021
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| US4649117A (en) * | 1985-03-15 | 1987-03-10 | Hoffmann-La Roche Inc. | Air lift bioreactor |
| WO2009034186A2 (en) * | 2007-09-13 | 2009-03-19 | Helmholtz-Zentrum für Infektionsforschung GmbH | Process for cell cultivation |
| WO2015176653A1 (en) * | 2014-05-22 | 2015-11-26 | 第三军医大学第三附属医院 | Bioreactor for three-dimensional tissue perfusion culture |
| CN209128459U (en) * | 2018-11-06 | 2019-07-19 | 天津赛尔康生物医药科技有限公司 | A kind of stem cell culture perfusing device |
| CN211546569U (en) * | 2020-01-06 | 2020-09-22 | 上海博威生物医药有限公司 | Perfusion device for stem cell culture |
| CN111778162A (en) * | 2020-07-29 | 2020-10-16 | 湖北明德健康科技有限公司 | Stem cell culture perfusion device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
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