CN211546569U - Perfusion device for stem cell culture - Google Patents
Perfusion device for stem cell culture Download PDFInfo
- Publication number
- CN211546569U CN211546569U CN202020014936.0U CN202020014936U CN211546569U CN 211546569 U CN211546569 U CN 211546569U CN 202020014936 U CN202020014936 U CN 202020014936U CN 211546569 U CN211546569 U CN 211546569U
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- Prior art keywords
- fixedly connected
- stem cell
- spring
- mixing
- cell culture
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 34
- 230000010412 perfusion Effects 0.000 title claims abstract description 26
- 238000004113 cell culture Methods 0.000 title claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 66
- 238000003756 stirring Methods 0.000 claims abstract description 32
- 238000002347 injection Methods 0.000 claims abstract description 6
- 239000007924 injection Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 24
- 239000000243 solution Substances 0.000 abstract description 16
- 239000007858 starting material Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model relates to a stem cell culture technical field just discloses a perfusion device for stem cell culture, including ration bottle, micro motor and pivot, the top of ration bottle and micro motor's bottom fixed connection. This a perfusion device for stem cell culture, through setting up the mixing case, first slide bar, the second slide bar, first spring, the second spring, the swivel mount, first dead lever, the second dead lever etc., mixing case is injected into at the culture solution, the stem cell is injected into mixing case back by the injection port, push down the mixing case, first spring and second spring are compressed, mixing bottom of the case portion "cross" draw-in groove cover is on the "cross" inserted bar on swivel mount top, the filling tube leaves the mixing case completely, rotate first dead lever and second dead lever, respectively with the screw hole on mixing case right side, left screw hole threaded connection, accomplish fixedly, the starter motor drives the swivel mount and rotates, mixing case rotates, the problem that present mixing stirring mode can destroy the stem cell in a large number has been solved.
Description
Technical Field
The utility model relates to a stem cell culture technical field specifically is a perfusion device for stem cell culture.
Background
After perfusion is performed on stem cells in a perfusion device for stem cell culture, a stirring rod is usually used for directly and violently stirring a stem cell-culture solution mixed solution for uniform mixing of the stem cells and the culture solution, so that a large amount of stem cells are broken, the culture effect is affected, and some components in the culture solution are often damaged due to stirring of traditional uniformly distributed stirring blades in the preparation process of the culture solution.
Disclosure of Invention
Technical problem to be solved
Not enough to prior art, the utility model provides a perfusion device for stem cell culture possesses the misce bene, reduces advantages such as the injury to stem cell, has solved the problem that current mixing stirring mode can destroy stem cell in a large number.
(II) technical scheme
For realizing above-mentioned misce bene, reduce to the stem cell purpose, the utility model provides a following technical scheme: a perfusion device for stem cell culture comprises a distribution bottle, a micro motor and a rotating shaft, wherein the top end of the distribution bottle is fixedly connected with the bottom end of the micro motor, the output end of the micro motor is fixedly connected with the top end of the rotating shaft, the surface of the rotating shaft is movably connected with the top wall of the distribution bottle, the bottom end of the rotating shaft is fixedly connected with a rotating disc, the bottom of the rotating disc is movably connected with the bottom inner wall of the distribution bottle, the side surface of the distribution bottle is fixedly connected with a first stirring plate, the surface of the rotating shaft is fixedly connected with a second stirring plate, the top wall of the distribution bottle is fixedly connected with a liquid taking pipe, the bottom end of the liquid taking pipe extends to the bottom of the distribution bottle, the top end of the liquid taking pipe is fixedly connected with a perfusion pump, the right side of the perfusion pump is communicated with a perfusion tube, the surface of the perfusion tube is movably connected with a, the bottom fixedly connected with swivel mount of first slide bar, the surface cover of first slide bar has first spring, and first spring and first slide bar swing joint, the top of first spring and the bottom fixed connection of mixing case, the bottom of first spring and the top fixed connection of swivel mount, the first pole setting of swivel mount top fixedly connected with, the internal thread of first pole setting is connected with first dead lever.
The bottom of the mixing box is movably connected with a second slide bar, the bottom end of the second slide bar is fixedly connected with the top end of a rotary seat, a second spring is sleeved on the surface of the second slide bar and is movably connected with the second slide bar, the top end of the second spring is fixedly connected with the bottom end of the mixing box, the bottom end of the second spring is fixedly connected with the top end of the rotary seat, a second vertical rod is fixedly connected with the top end of the rotary seat, a second fixing rod is connected with the inner thread of the second vertical rod, a supporting seat is movably connected with the bottom of the rotary seat, a motor is fixedly connected with the bottom end of the supporting seat, the output end of the motor is fixedly connected with the bottom of the rotary seat, the output end of the motor is movably connected with the supporting seat, a liquid outlet pump is communicated with the back of the mixing box, a liquid outlet pipe is communicated with the back of, the top end of the distribution bottle is provided with a liquid inlet.
Preferably, a through hole is formed in the surface of the first stirring plate.
Preferably, the specification of the second stirring plate is the same as that of the first stirring plate.
Preferably, threaded hole is seted up on the right side of mixing case, and threaded hole is seted up on the left side of mixing case, the bottom of mixing case has seted up "cross" draw-in groove.
Preferably, the size of the first fixing rod is matched with the size of a threaded hole in the right side of the blending box.
Preferably, the size of the second fixing rod is matched with the size of a threaded hole in the left side of the blending box.
Preferably, the swivel mount top is equipped with "cross" inserted bar, and the size of this inserted bar and the size phase-match of mixing bottom of the case "cross" draw-in groove.
Compared with the prior art, the utility model provides a perfusion device for stem cell culture possesses following beneficial effect:
1. this a perfusion device for stem cell culture, through setting up the mixing case, first slide bar, the second slide bar, first spring, the second spring, the swivel mount, first dead lever, the second dead lever etc., mixing case is injected into at the culture solution, the stem cell is injected into mixing case back by the injection port, push down the mixing case, first spring and second spring are compressed, the "cross" draw-in groove cover of mixing bottom of the case portion is on the "cross" inserted bar on swivel mount top, the filling tube leaves the mixing case completely, rotate first dead lever and second dead lever, respectively with the screw hole on mixing case right side, left screw hole threaded connection, accomplish fixedly, the starter motor drives the swivel mount and rotates, the mixing case rotates, make the mild mixing of stem cell and culture solution of mixing incasement portion, the problem that present mixing stirring mode can destroy stem cell in a large number has been solved.
2. This a perfusion device for stem cell culture, through setting up first stirring board, second stirring rod and carousel, the through hole has been seted up on the surface of first stirring board and second stirring board, the direct probability that collides with the stirring board of composition in the culture solution has been reduced, because first stirring board sets up the latter half at the ration bottle, can drive the liquid level rotation of bottommost, the second stirring board sets up and can drive the liquid level rotation of top at the first half of ration bottle, through setting up minimum quantity's stirring board, the mesh of mixing culture solution has been reached, the destroyed probability of culture solution composition has been reduced.
Drawings
FIG. 1 is a sectional view of the front view structure of the present invention;
fig. 2 is a schematic rear view of the present invention;
fig. 3 is an enlarged view of a portion of a in fig. 1 according to the present invention.
Wherein: 1. a dispensing bottle; 2. a micro motor; 3. a rotating shaft; 4. a second stirring plate; 5. a turntable; 6. a first stirring plate; 7. a liquid taking pipe; 8. a perfusion pump; 9. a perfusion tube; 10. a blending box; 11. a first slide bar; 12. a first spring; 13. rotating; 14. a motor; 15. a first upright rod; 16. a first fixing lever; 17. a second upright stanchion; 18. a second fixing bar; 19. a liquid inlet; 20. an injection port; 21. a second slide bar; 22. a second spring; 23. a liquid outlet pump; 24. a liquid outlet pipe; 25. and (4) supporting the base.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a perfusion device for stem cell culture, comprising a dispensing bottle 1, a micro motor 2 and a rotating shaft 3, wherein the top end of the dispensing bottle 1 is fixedly connected to the bottom end of the micro motor 2, the output end of the micro motor 2 is fixedly connected to the top end of the rotating shaft 3, the surface of the rotating shaft 3 is movably connected to the top wall of the dispensing bottle 1, the bottom end of the rotating shaft 3 is fixedly connected to a rotating disc 5, the bottom of the rotating disc 5 is movably connected to the bottom inner wall of the dispensing bottle 1, the side surface of the dispensing bottle 1 is fixedly connected to a first stirring plate 6, the surface of the first stirring plate 6 is provided with a through hole, the surface of the rotating shaft 3 is fixedly connected to a second stirring plate 4, the specification of the second stirring plate 4 is the same as that of the first stirring plate 6, the top wall of the dispensing bottle 1 is fixedly connected to a liquid-taking pipe 7, a filling pump 8 is fixedly connected to the top end of the liquid taking pipe 7, a filling pipe 9 is communicated with the right side of the filling pump 8, the surface of the filling pipe 9 is movably connected with a blending box 10, a threaded hole is formed in the right side of the blending box 10, a threaded hole is formed in the left side of the blending box 10, a cross-shaped clamping groove is formed in the bottom of the blending box 10, a first sliding rod 11 is movably connected to the bottom of the blending box 10, a swivel base 13 is fixedly connected to the bottom end of the first sliding rod 11, a cross-shaped inserting rod is arranged at the top end of the swivel base 13, the size of the inserting rod is matched with that of the cross-shaped clamping groove in the bottom of the blending box 10, a first spring 12 is sleeved on the surface of the first sliding rod 11, the first spring 12 is movably connected to the first sliding rod 11, the top end of the first spring 12 is fixedly connected to the bottom of the blending box 10, the bottom end of, the internal thread of the first upright rod 15 is connected with a first fixing rod 16, and the size of the first fixing rod 16 is matched with the size of a threaded hole on the right side of the blending box 10.
The bottom of the blending box 10 is movably connected with a second slide bar 21, the bottom end of the second slide bar 21 is fixedly connected with the top end of a rotary seat 13, the surface of the second slide bar 21 is sleeved with a second spring 22, the second spring 22 is movably connected with the second slide bar 21, the top end of the second spring 22 is fixedly connected with the bottom end of the blending box 10, the bottom end of the second spring 22 is fixedly connected with the top end of the rotary seat 13, the top end of the rotary seat 13 is fixedly connected with a second vertical rod 17, the inner thread of the second vertical rod 17 is connected with a second fixing rod 18, the size of the second fixing rod 18 is matched with the size of a threaded hole at the left side of the blending box 10, the bottom of the rotary seat 13 is movably connected with a support seat 25, the bottom end of the support seat 25 is fixedly connected with a motor 14, the output end of the motor 14 is fixedly connected with the bottom of the rotary seat, the back of the liquid outlet pump 23 is communicated with a liquid outlet pipe 24, the top end of the blending box 10 is provided with an injection port 20, and the top end of the dispensing bottle 1 is provided with a liquid inlet 19.
When the device is used, components of a culture solution are poured into the distribution bottle 1 through the solution inlet 19, the micro motor 2 is started, the rotating shaft 3 rotates, the filling pump 8 is started, the culture solution is injected into the blending box 10, stem cells are injected into the blending box 10 through the injection port 20, the blending box 10 is pressed downwards, the first spring 12 and the second spring 22 are compressed, the cross-shaped clamping groove at the bottom of the blending box 10 is sleeved on the cross-shaped inserting rod at the top end of the rotary seat 13, the filling pipe 9 completely leaves the blending box 10, the first fixing rod 16 and the second fixing rod 18 are rotated to be respectively in threaded connection with the threaded hole at the right side and the threaded hole at the left side of the blending box 10, after the fixation is completed, the starting motor 14 drives the rotary seat 13 to rotate, so that the stem cells and the culture solution in the mixing box 10 are mixed mildly, the liquid outlet pump 23 is started to discharge the stem cell-culture solution mixed liquid, and the next culture operation is carried out.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. Perfusion device for stem cell culture, comprising a distribution bottle (1), a micromotor (2) and a rotating shaft (3), characterized in that: the top end of the dispensing bottle (1) is fixedly connected with the bottom end of the micro motor (2), the output end of the micro motor (2) is fixedly connected with the top end of the rotating shaft (3), the surface of the rotating shaft (3) is movably connected with the top wall of the dispensing bottle (1), the bottom end of the rotating shaft (3) is fixedly connected with the rotating disc (5), the bottom of the rotating disc (5) is movably connected with the bottom inner wall of the dispensing bottle (1), the side surface of the dispensing bottle (1) is fixedly connected with the first stirring plate (6), the surface of the rotating shaft (3) is fixedly connected with the second stirring plate (4), the top wall of the dispensing bottle (1) is fixedly connected with the liquid taking pipe (7), the bottom end of the liquid taking pipe (7) extends to the bottom of the dispensing bottle (1), the top end of the liquid taking pipe (7) is fixedly connected with the filling pump (8), the right side of the filling pump (8) is communicated with the, the surface of the filling pipe (9) is movably connected with a mixing box (10), the bottom of the mixing box (10) is movably connected with a first sliding rod (11), the bottom end of the first sliding rod (11) is fixedly connected with a rotating base (13), a first spring (12) is sleeved on the surface of the first sliding rod (11), the first spring (12) is movably connected with the first sliding rod (11), the top end of the first spring (12) is fixedly connected with the bottom of the mixing box (10), the bottom end of the first spring (12) is fixedly connected with the top end of the rotating base (13), the top end of the rotating base (13) is fixedly connected with a first vertical rod (15), and the inner thread of the first vertical rod (15) is connected with a first fixing rod (16);
the bottom swing joint of mixing case (10) has second slide bar (21), the bottom of second slide bar (21) and the top fixed connection of swivel mount (13), the surface cover of second slide bar (21) has second spring (22), and second spring (22) and second slide bar (21) swing joint, the top of second spring (22) and the bottom fixed connection of mixing case (10), the bottom of second spring (22) and the top fixed connection of swivel mount (13), the top fixedly connected with second pole setting (17) of swivel mount (13), the internal thread of second pole setting (17) is connected with second dead lever (18), the bottom swing joint of swivel mount (13) has supporting seat (25), the bottom fixedly connected with motor (14) of supporting seat (25), the output of motor (14) and the bottom fixed connection of swivel mount (13), and the output of motor (14) and supporting seat (25) swing joint, the back intercommunication of mixing box (10) has out liquid pump (23), the back intercommunication of going out liquid pump (23) has drain pipe (24), the top of mixing box (10) is equipped with injection mouth (20), the top of ration bottle (1) is equipped with into liquid mouth (19).
2. A perfusion device for stem cell culture according to claim 1, wherein: the surface of the first stirring plate (6) is provided with a through hole.
3. A perfusion device for stem cell culture according to claim 1, wherein: the specification of the second stirring plate (4) is the same as that of the first stirring plate (6).
4. A perfusion device for stem cell culture according to claim 1, wherein: threaded hole is seted up on the right side of mixing case (10), and threaded hole is seted up on the left side of mixing case (10), the bottom of mixing case (10) has seted up "cross" draw-in groove.
5. A perfusion device for stem cell culture according to claim 1, wherein: the size of the first fixing rod (16) is matched with the size of a threaded hole in the right side of the blending box (10).
6. A perfusion device for stem cell culture according to claim 1, wherein: the size of the second fixing rod (18) is matched with the size of a threaded hole in the left side of the blending box (10).
7. A perfusion device for stem cell culture according to claim 1, wherein: the top end of the rotary seat (13) is provided with a cross-shaped inserted bar, and the size of the inserted bar is matched with the size of a cross-shaped clamping groove at the bottom of the blending box (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020014936.0U CN211546569U (en) | 2020-01-06 | 2020-01-06 | Perfusion device for stem cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020014936.0U CN211546569U (en) | 2020-01-06 | 2020-01-06 | Perfusion device for stem cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211546569U true CN211546569U (en) | 2020-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020014936.0U Expired - Fee Related CN211546569U (en) | 2020-01-06 | 2020-01-06 | Perfusion device for stem cell culture |
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| Country | Link |
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| CN (1) | CN211546569U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877210A (en) * | 2021-02-25 | 2021-06-01 | 孔祥玲 | Stem cell culture perfusion device with controllable pH value |
-
2020
- 2020-01-06 CN CN202020014936.0U patent/CN211546569U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877210A (en) * | 2021-02-25 | 2021-06-01 | 孔祥玲 | Stem cell culture perfusion device with controllable pH value |
| CN112877210B (en) * | 2021-02-25 | 2022-07-26 | 上海揽微赛尔生物科技有限公司 | Stem cell culture perfusion device with controllable pH value |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200922 |