CN112859316A - Large-flux microscope slide loading system and scanning method - Google Patents
Large-flux microscope slide loading system and scanning method Download PDFInfo
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- CN112859316A CN112859316A CN202011606108.7A CN202011606108A CN112859316A CN 112859316 A CN112859316 A CN 112859316A CN 202011606108 A CN202011606108 A CN 202011606108A CN 112859316 A CN112859316 A CN 112859316A
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000011521 glass Substances 0.000 claims description 25
- 230000004907 flux Effects 0.000 claims description 12
- 238000003860 storage Methods 0.000 claims description 7
- 239000000969 carrier Substances 0.000 claims description 4
- 230000002146 bilateral effect Effects 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 230000000903 blocking effect Effects 0.000 claims description 2
- 230000008859 change Effects 0.000 claims description 2
- 210000001503 joint Anatomy 0.000 claims description 2
- 230000000994 depressogenic effect Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 15
- 230000008569 process Effects 0.000 abstract description 10
- 230000007246 mechanism Effects 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000001915 proofreading effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
Abstract
The invention discloses a large-flux microscope slide loading system and a scanning method, and belongs to the technical field of microscope examination. The invention discloses a large-flux microscope slide loading system, which comprises an object stage, a microscope body, a slide box and a slide carrier, wherein the object stage is provided with a slide groove; the slide carrier is provided with at least two loading grooves capable of placing slides, and the moving direction of the slide carrier in the slide box is parallel to the moving direction of the slide carrier on the object stage; and a push-pull piece is assembled on the object stage. Through continuous exploration and test, the slide carrier capable of accommodating a plurality of slides at one time is arranged, so that the slide scanning efficiency is effectively improved; the slide carrier can be moved to the lower part of the microscope body only by moving once, and has the advantages of simple moving process, practical structure and low manufacturing cost. The slide carrier drives the slide to move, so that compared with the method of directly operating the slide, the slide carrier can effectively protect the slide and avoid the slide from being damaged, and the slide carrier has the advantages of simple structure, practicality and ingenious conception.
Description
Technical Field
The invention relates to a large-flux microscope slide loading system and a scanning method, and belongs to the technical field of microscope examination.
Background
Chinese patent (CN 210243508U) discloses a full-automatic microscope device, which comprises a microscope and a bottom plate component, and further comprises a microscope component, a slide loading component and a slide recovery component, wherein the microscope component is arranged on the bottom plate component, the microscope is correspondingly arranged above the microscope component, the slide loading component is correspondingly arranged on one side of the microscope component, and the slide recovery component is correspondingly arranged on the other side of the microscope component. The microscopic examination part comprises a microscopic examination objective table, a mounting plate, a shifting mechanism and a shifting plate, wherein the mounting plate is arranged on the microscopic examination objective table, and an L-shaped groove channel is arranged on the mounting plate; the inlet of the groove channel is positioned on one side of the mounting plate and is provided with a trumpet-shaped opening, the outlet is positioned at one end of the mounting plate, and the groove channel is also provided with a rectangular through hole; the poking plate is connected with the poking mechanism, the poking mechanism is arranged on the mounting plate, and the poking plate is driven to slide back and forth along the bending part and the outlet of the groove channel.
According to the scheme, after the glass slides are sleeved in the glass slide storage component of the glass slide loading component, the linear motor component drives the poking piece to push the bottommost glass slide of the glass slide storage component to be sleeved in the groove through groove of the mounting plate along the through groove; the poking mechanism drives the poking plate to push the slide suit to the rectangular through hole.
However, the above solution has the following problems:
first, only one slide can be scanned at a time, resulting in inefficient scanning.
Secondly, slide suit needs longitudinal movement earlier, and then lateral shifting, and supporting moving structure is comparatively complicated, and the part that needs is many, and manufacturing cost is high.
Thirdly, the slide is directly operated, so that the slide is easily damaged and lost.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a slide carrier which can contain a plurality of slides at one time, so that the slide scanning efficiency is effectively improved; meanwhile, the moving direction of the slide carrier in the slide box is parallel to the moving direction of the slide carrier on the objective table, so that the slide carrier can be moved to the lower part of the microscope body only by moving once, the moving process is simple, the structure is practical, and the manufacturing cost is low; the slide carrier drives the slide to move, and compared with a method of directly operating the slide, the slide loading system and the scanning method of the high-flux microscope slide can effectively protect the slide and avoid the slide from being damaged.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a large-flux microscope slide loading system,
comprises an object stage with a plate piece, a microscope body capable of observing a sample, a slide box assembled adjacent to the object stage, and a slide carrier capable of placing a slide;
the microscope body is suspended above the objective table;
the slide cassette having a cassette cavity capable of holding at least one slide carrier;
one end of the box cavity adjacent to the object stage is provided with an opening;
the opening faces to the plate part of the objective table;
the slide carrier is provided with at least two loading grooves capable of placing slides, and the moving direction of the slide carrier in the slide box is parallel to the moving direction of the slide carrier on the object stage;
a through hole is formed in the loading groove;
a push-pull piece capable of driving the slide carrier to translate is assembled on the objective table;
the push-pull piece can adsorb or hook or adhere to or scarf joint the slide carrier;
a plurality of clamping grooves for erecting slide carriers are arranged in the cavity of the slide box;
the clamping grooves are protruded out of the inner cavity wall of the slide box and are arranged in the slide box in a bilateral symmetry mode.
Through continuous exploration and test, the slide carrier capable of accommodating a plurality of slides at one time is arranged, so that the slide scanning efficiency is effectively improved; meanwhile, the moving direction of the slide carrier in the slide box is parallel to the moving direction of the slide carrier on the objective table, so that the slide carrier can be moved to the lower part of the microscope body only by moving once, the moving process is simple, the structure is practical, and the manufacturing cost is low.
The slide carrier drives the slide to move, so that compared with the method of directly operating the slide, the slide carrier can effectively protect the slide and avoid the slide from being damaged, and the slide carrier has the advantages of simple structure, practicality and ingenious conception.
As a preferable technical measure:
the push-pull piece is connected with the plate piece of the objective table in a sliding way through a slide rail component;
the sliding rail assembly is provided with a driving source capable of driving the push-pull piece to reciprocate on the plate piece, the scheme is feasible, and production and manufacturing are facilitated.
As a preferable technical measure:
an I-shaped part is arranged at one end of the push-pull piece assembled with the slide carrier;
the slide carrier is provided with a T-shaped hole matched with the I-shaped part;
the I-shaped part can be embedded into the T-shaped hole, so that the push-pull piece and the slide carrier are fixedly connected in the horizontal direction.
According to the characteristic that the slide carrier only needs to move horizontally, the I-shaped part and the T-shaped hole which can be matched with each other are arranged, so that the push-pull piece and the slide carrier can be flexibly and fixedly connected in the horizontal direction, the structure is simple and practical, and the conception is ingenious.
As a preferable technical measure:
the objective table is connected with the microscope body through the adapter,
the lower end part of the focusing unit is connected with a focusing unit which can drive the objective table to reciprocate;
the focusing unit is fixedly connected with a focusing knob of the microscope body;
the object stage comprises a transversely moving transverse moving component and a longitudinally moving longitudinal moving component;
the transverse moving component is assembled below the push-pull component, and the longitudinal moving component is assembled below the transverse moving component, so that transverse movement and longitudinal movement of the plate surface piece are realized;
the objective table is driven by the focusing unit to move up and down to realize slide focusing.
The slide taking and returning push-pull piece, the focusing unit and the moving assembly are arranged on the objective table, so that the integrity and reliability of the imaging optical system are ensured.
As a preferable technical measure:
a slide magazine capable of holding slide cassettes is also included to further increase the storage capacity of slides;
the lower end part of the slide magazine is provided with a lifting component capable of driving the slide box to lift, and the lifting component is matched with the push-pull piece, so that the I-shaped part can automatically fall into the T-shaped hole, the whole process is automatically completed, the automation degree of the slide magazine is improved, the slide magazine is convenient to use, and the loading efficiency is further improved.
As a preferable technical measure:
the lifting assembly is provided with a bottom plate for fixing the slide box;
one of the bottom plate and the glass box is provided with a groove, and the other is provided with a bulge;
the bulges can be embedded into the grooves, so that the glass sheet box is fixed on the lifting assembly, and the glass sheet box is simple and practical in structure and convenient to produce and manufacture.
As a preferable technical measure:
the two end parts of the loading groove are downwards sunken to form a longitudinally extending blocking part for erecting the glass slide, the middle area of the loading groove is provided with the through hole, and a limiting part capable of moving back and forth is arranged between the adjacent through holes;
at least one part of the limiting piece is positioned in the through hole and can move in the through hole so as to change the loading space of the loading groove.
The invention arranges the limiting part which can move back and forth on the chassis part, realizes the effective limiting of the slide, has simple structure, low manufacturing cost, small occupied space, light weight, convenient popularization and use, ingenious conception and feasible scheme.
The limiting piece is arranged on the slide carrier through an elastic piece;
the elastic part is a pressure spring, an air rod or a hydraulic rod, and the elastic part realizes the movement and reset of part or all of the limiting part in the through hole;
one end of the limiting piece, which is adjacent to the glass slide, is provided with an inverted trapezoidal surface structure, and the upper surface of the inverted trapezoidal surface structure is provided with a toggle key;
the upper end of the inverted step surface structure protrudes out of the upper surface of the loading groove or is flush with the upper surface of the loading groove.
This locating part has the design of falling the trapezoidal face with slide contact one side, can guarantee the convenience that the slide put into and guarantee the fixity after the slide is put into simultaneously, specifically including but not limited to remove, drop.
The toggle key protrudes out of the upper surface of the loading groove; when placing the slide, the user can push the limiting piece backwards through the toggle key, so that the slide placing area is enlarged.
The upper surface of the loading groove is provided with a socket for connecting the toggle key in a penetrating way,
the size of the interface is matched with the moving range of the toggle key.
As a preferable technical measure:
the lower end of the slide carrier is provided with a plurality of sunken parts for accommodating the limiting parts;
the lower end of the concave part is provided with a connecting strip for covering the lower end opening of the concave part;
the connecting strip piece is fixed on the slide carrier and extends to the lower part of the limiting part, the upper wall surface of the connecting strip piece and the lower wall surface of the chassis part form an installation cavity for assembling the limiting part, and therefore the upper and lower moving ranges of the limiting part can be effectively limited, and the slide carrier is simple in structure and practical.
The upper wall surface of the connecting strip and the lower wall surface of the slide carrier form an installation cavity for assembling the limiting part.
As a preferable technical measure:
a method for scanning a large-flux microscope slide,
applying a high flux microscope slide loading system as described above;
it comprises the following contents:
the distance of the slide moving at each time is less than one window distance, so that the pictures shot at each time have an overlapped area;
then identifying the overlapping area of the slide pictures, and cutting or splicing the pictures to obtain a complete slide picture;
the method comprises the steps of pre-scanning a slide, preliminarily fitting a theoretical focusing position of the slide by shooting a plurality of points on the slide, and reducing the vertical axis movement distance when each point is shot subsequently.
Due to errors in stage motion, there is some duplication or misalignment of the images taken after each motion, resulting in loss or error of digital slide information.
Through continuous exploration and test, the invention ensures that each picture is shot to have an overlapped area by ensuring that the slide moves less than one window distance each time, and then the pictures are spliced or cut after the overlapped area is identified.
Furthermore, the shape of the slide has certain geometric errors and deformation, and when a plurality of slides are carried, the vertical axis movement distance needs to be increased in order to realize accurate focusing of each point of each slide, so that the slide scanning time is increased.
The invention adds a pre-scanning process before each point is scanned and shot, preliminarily fits the theoretical focusing position of the glass slide by shooting a plurality of points on the glass slide, and reduces the vertical axis movement distance when each point is shot subsequently.
As a preferable technical measure:
the method for shooting by adopting pre-scanning specifically comprises the following steps:
firstly, acquiring and checking information of a to-be-scanned film;
(proofreading of rank position and manually input case slide information)
Secondly, moving the object stage to a preset position;
thirdly, the push-pull piece moves towards the upper part of the slide carrier;
fourthly, the slide box drives the slide carrier to move upwards, so that the I-shaped part of the push-pull piece falls into a T-shaped hole of the slide carrier;
fifthly, the slide carrier and the slide are driven by the push-pull piece to move to the lower part of the microscope body;
sixthly, adjusting the position of the microscope body, and prescanning a plurality of points on the slide;
and seventhly, performing coarse focusing according to the pre-scanning data: moving back to the objective lens;
eighthly, finding the clearest position of the rough focusing;
and ninthly, fine focusing is performed: moving towards the objective lens;
step ten, finding the clearest position of the fine focusing;
step ten, calculating the vertical moving position of the microscope body according to the clearest fine focusing positions of the plurality of points which are pre-scanned;
and step twelve, switching the high power objective lens by the microscope body, moving to the focusing starting point, moving towards the end point, finding the clearest position, and taking a picture for storage.
The invention has detailed scheme, reasonable procedure and convenient programming realization.
Compared with the prior art, the invention has the following beneficial effects:
through continuous exploration and test, the slide carrier capable of accommodating a plurality of slides at one time is arranged, so that the slide scanning efficiency is effectively improved; meanwhile, the moving direction of the slide carrier in the slide box is parallel to the moving direction of the slide carrier on the objective table, so that the slide carrier can be moved to the lower part of the microscope body only by moving once, the moving process is simple, the structure is practical, and the manufacturing cost is low.
The slide carrier drives the slide to move, so that compared with the method of directly operating the slide, the slide carrier can effectively protect the slide and avoid the slide from being damaged, and the slide carrier has the advantages of simple structure, practicality and ingenious conception.
Drawings
FIG. 1 is a view showing the overall structure of the present invention;
FIG. 2 is a view of the structure of FIG. 1, shown inverted at an angle;
FIG. 3 is a view of the structure of FIG. 2, shown inverted at an angle;
FIG. 4 is a schematic view of the present invention showing the structure of the stage assembly push-pull member;
fig. 5 is a view showing the construction of the cassette of the present invention;
fig. 6 is a schematic view of a slide cassette equipped with slide carriers of the present invention;
FIG. 7 is a schematic view of one configuration of a slide carrier of the present invention;
FIG. 8 is a diagram of a position limiting element according to the present invention;
FIG. 9 is an exploded view of a slide carrier of the present invention;
FIG. 10 is a schematic view of a slide carrier of the present invention showing a slide placement;
FIG. 11 is an exploded view of the slide carrier of the present invention with the addition of a connecting strip;
FIG. 12 is an assembled view of the structure shown in FIG. 11;
FIG. 13 is a structural view of the slide glass of the structure shown in FIG. 12.
Description of reference numerals:
1. an object stage; 2. a microscope body; 3. a cassette for glass sheets; 4. a slide carrier; 5. a film library; 6. glass slide; 7. a push-pull member; 71. an I-shaped portion; 11. a panel member; 12. a traversing assembly; 13. a longitudinal movement component; 14. a focusing unit; 15. a slide rail assembly; 31. opening the mouth; 32. a card slot; 41. a chassis member; 42. a limiting member; 43. a loading slot; 44. an elastic member; 45. a guide pin; 47. connecting strips; 48. a T-shaped hole; 411. an interface; 421. an inverted trapezoidal surface structure; 422. dialing a key; 4211. an upper edge; 431. a stopper; 432. and a through hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
It should be noted that when two elements are "fixedly connected" or "slidably connected", the two elements may be directly connected or intervening elements may also be present. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements present. The terms "horizontal," "vertical," "up," "down," and the like are used herein for illustrative purposes only.
As shown in fig. 1-13, a high throughput microscope slide loading system,
comprises an object stage 1 with a plate member 11, a microscope body 2 capable of observing a sample, a slide box 3 assembled adjacent to the object stage 1, and a slide carrier 4 capable of placing a slide 6;
the microscope body 2 is suspended above the objective table 1;
the cassette 3 is provided with a cassette cavity capable of accommodating at least one slide carrier 4;
one end of the box cavity adjacent to the object stage 1 is provided with an opening 31;
the opening 31 faces the plate member 11 of the object stage 1;
the slide carrier 4 is provided with at least two loading grooves 43 capable of placing slides, and the moving direction of the slide carrier in the slide box 3 is parallel to the moving direction of the slide carrier on the object stage 1;
a through hole 432 is formed in the loading groove 43;
a push-pull piece 7 capable of driving the slide carrier to translate is assembled on the objective table 1;
the push-pull piece 7 can adsorb or hook or stick or scarf the slide carrier;
a plurality of clamping grooves 32 for erecting slide carriers 4 are arranged in the cavity of the slide box 3;
the clamping grooves 32 protrude out of the inner cavity wall of the slide box 3 and are arranged in the slide box 3 in a bilateral symmetry manner.
Through continuous exploration and test, the slide carrier 4 capable of accommodating a plurality of slides 6 at one time is arranged, so that the slide scanning efficiency is effectively improved; meanwhile, the moving direction of the slide carrier 4 in the slide box 3 is parallel to the moving direction of the slide carrier on the objective table 1, so that the slide carrier 4 can be moved to the lower part of the microscope body 2 only by moving once, and the slide carrier moving device is simple in moving process, practical in structure and low in manufacturing cost.
The slide carrier 4 is used for driving the slide to move, and compared with the method for directly operating the slide, the slide carrier can effectively protect the slide and avoid the slide from being damaged, and has the advantages of simple structure, practicality and ingenious conception.
The objective table 1 is connected with the microscope body 2 through a connector,
the lower end part of the focusing unit is connected with a focusing unit 14 which can drive the objective table 1 to reciprocate;
the focusing unit 14 is fixedly connected with a focusing knob of the microscope body 2;
the object stage 1 comprises a transversely-moving transverse-moving component 12 and a longitudinally-moving longitudinal-moving component 13;
the transverse moving component 12 is assembled below the push-pull component 7, and the longitudinal moving component 13 is assembled below the transverse moving component 12, so that transverse movement and longitudinal movement of the plate piece 11 are realized;
the objective table 1 drives the focusing unit 14 to move up and down to realize the focusing of the slide.
The push-pull piece 7 for taking and returning the slide, the focusing unit 14 and the moving assembly are arranged on the objective table 1, so that the integrity and reliability of an imaging optical system are ensured.
The invention adds a concrete embodiment of the sliding rail component 15:
the push-pull piece 7 is connected with the plate piece 11 of the objective table 1 in a sliding way through a slide rail assembly 15;
the sliding rail assembly 15 is provided with a driving source capable of driving the push-pull member 7 to reciprocate on the plate member 11 and a stopper capable of limiting the push-pull member 7, so that the scheme is feasible and the production and the manufacture are convenient.
The embodiment of the connection mode of the push-pull piece 7 and the slide carrier 4 of the invention comprises:
an I-shaped part 71 is arranged at one end of the push-pull piece 7 assembled with the slide carrier 4;
the slide carrier 4 is provided with a T-shaped hole 48 matched with the I-shaped part 71;
the I-shaped part can be embedded into the T-shaped hole 48, so that the push-pull piece 7 and the slide carrier 4 are fixedly connected in the horizontal direction.
According to the characteristic that the slide carrier 4 only needs to move horizontally, the I-shaped part and the T-shaped hole 48 which can be matched with each other are arranged, so that the push-pull piece 7 and the slide carrier 4 can be flexibly and fixedly connected in the horizontal direction, and the slide carrier is simple in structure, practical and ingenious in conception.
The invention adds a specific embodiment of the film library 5:
a slide magazine 5 capable of holding slide cassettes 3 is also included to further increase the storage capacity of slides;
the lower end part of the slide magazine 5 is provided with a lifting component capable of driving the slide box 3 to lift, and the lifting component is matched with the push-pull piece 7, so that the I-shaped part can automatically fall into the T-shaped hole 48, the whole process is automatically completed, the automation degree of the invention is improved, the use is convenient, and the loading efficiency is further improved.
The lifting assembly is provided with a bottom plate for fixing the slide box 3;
one of the bottom plate and the slide box 3 is provided with a groove, and the other is provided with a bulge;
the bulges can be embedded into the grooves, so that the glass sheet box 3 is fixed on the lifting assembly, and the glass sheet box is simple and practical in structure and convenient to produce and manufacture.
One embodiment of the structure of the slide carrier 4 of the present invention:
the slide carrier 4 is provided with a hollow chassis member 41; the chassis 41 is provided with the loading slot 43 and is provided with a through hole for passing through
Laterally arranged guide pins 45 of the elastic member 44 are provided.
The loading slot 43 has two ends recessed downward to form laterally extending stops 431 for mounting a slide, and a through hole 432 is formed in the middle region thereof, and a stopper 42 capable of reciprocating is provided adjacent to the through hole 432.
The limiting piece 42 capable of moving back and forth is arranged on the base plate piece 41, so that effective limiting of the glass slide is realized, the structure is simple, the manufacturing cost is low, the occupied space is small, the weight is light, the glass slide limiting device is convenient to popularize and use, the conception is ingenious, and the scheme is feasible.
The limiting member 42 is arranged on the slide carrier 4 through an elastic member 44;
the elastic element 44 is a pressure spring, an air rod or a hydraulic rod, which realizes that part or all of the limiting element 42 moves and resets in the through hole 432;
one end of the limiting piece 42 adjacent to the slide is provided with an inverted trapezoidal surface structure 421, and the upper surface of the inverted trapezoidal surface structure is provided with a toggle key 422;
the upper edge 4211 of the inverted tread structure 421 protrudes from the upper surface of the loading slot 43 or is flush with the upper surface of the loading slot 43.
The stopper 42 has an inverted step design on the side contacting the slide, so that the convenience of putting the slide can be ensured, and the fixation of the slide after putting the slide can be ensured, specifically including but not limited to moving and falling.
The toggle key 422 protrudes out of the upper surface of the loading slot 43; when a user places a slide, the user can push the limiting piece 42 backwards by dialing the key 422, so that the slide placing area is enlarged.
The upper surface of the loading slot 43 is provided with a socket 411 for connecting the toggle key 422 in a penetrating way,
the size of the socket 411 is matched with the moving range of the toggle key 422.
One embodiment of the structure of the slide carrier 4 of the present invention:
the lower end of the slide carrier 4 is provided with a plurality of concave parts for accommodating the limiting parts 42;
the lower end of the concave part is provided with a connecting strip 47 for covering the lower end opening 31 of the concave part;
the connecting strip 47 is fixed on the slide carrier 4 and extends to the lower part of the limiting part 42, an installation cavity for assembling the limiting part 42 is formed by the upper wall surface of the connecting strip 47 and the lower wall surface of the chassis part 41, and therefore the upper and lower moving ranges of the limiting part 42 can be effectively limited, and the slide carrier is simple in structure and practical.
The upper wall surface of the connecting strip 47 and the lower wall surface of the slide carrier 4 form a mounting cavity for mounting the stopper 42.
Application of the invention a specific embodiment of a large flux microscope slide loading system as described above:
a method for scanning a large-flux microscope slide,
it comprises the following contents:
the distance of the slide moving at each time is less than one window distance, so that the pictures shot at each time have an overlapped area;
then identifying the overlapping area of the slide pictures, and cutting or splicing the pictures to obtain a complete slide picture;
the method comprises the steps of pre-scanning a slide, preliminarily fitting a theoretical focusing position of the slide by shooting a plurality of points on the slide, and reducing the vertical axis movement distance when each point is shot subsequently.
The slide carrier 4 capable of accommodating a plurality of slides at one time is arranged, so that the slide scanning efficiency can be effectively improved; meanwhile, the moving direction of the slide carrier 4 in the slide box 3 is parallel to the moving direction of the slide carrier on the objective table 1, so that the slide carrier 4 can be moved to the lower part of the microscope body 2 only by moving once, and the slide carrier moving device is simple in moving process, practical in structure and low in manufacturing cost.
The slide carrier 4 is used for driving the slide to move, and compared with the method for directly operating the slide, the slide carrier can effectively protect the slide and avoid the slide from being damaged, and has the advantages of simple structure, practicality and ingenious conception.
Further, since there is an error in the movement of the stage 1, there is a certain repetition or misalignment of the captured image after each movement, resulting in a loss or error of the digital slide information.
Through continuous exploration and test, the invention ensures that each picture is shot to have an overlapped area by ensuring that the slide moves less than one window distance each time, and then the pictures are spliced or cut after the overlapped area is identified.
Furthermore, the shape of the slide has certain geometric errors and deformation, and when a plurality of slides are carried, the vertical axis movement distance needs to be increased in order to realize accurate focusing of each point of each slide, so that the slide scanning time is increased.
The invention adds a pre-scanning process before each point is scanned and shot, preliminarily fits the theoretical focusing position of the glass slide by shooting a plurality of points on the glass slide, and reduces the vertical axis movement distance when each point is shot subsequently.
One specific embodiment of slide scanning of the present invention:
the method for shooting by adopting pre-scanning specifically comprises the following steps:
firstly, acquiring and checking information of a to-be-scanned film;
(proofreading of rank position and manually input case slide information)
Secondly, moving the object stage 1 to a preset position;
thirdly, the push-pull piece 7 moves to the upper part of the slide carrier 4;
fourthly, the slide box 3 drives the slide carrier 4 to move upwards, so that the I-shaped part of the push-pull piece 7 falls into the T-shaped hole 48 of the slide carrier 4;
fifthly, the push-pull piece 7 drives the slide carrier 4 and the slide to move to the lower part of the microscope body 2;
sixthly, adjusting the position of the microscope body 2, and prescanning a plurality of points on the slide;
and seventhly, performing coarse focusing according to the pre-scanning data: moving back to the objective lens;
eighthly, finding the clearest position of the rough focusing;
and ninthly, fine focusing is performed: moving towards the objective lens;
step ten, finding the clearest position of the fine focusing;
step ten, calculating the vertical moving position of the microscope body 2 according to the clearest fine focusing positions of the plurality of points which are pre-scanned;
and step twelve, the microscope body 2 switches the high power objective lens, moves to the focusing starting point and moves towards the end point, finds the clearest position, and takes a picture for storage.
The invention has detailed scheme, reasonable procedure and convenient programming realization.
In the present application, the fastening or fixing connection mode may be a screw connection, a welding, a riveting, an inserting connection, or a connection through a third component, and those skilled in the art may select the fastening or fixing connection mode according to actual situations.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A high flux microscope slide loading system characterized in that,
comprises an object stage (1) with a plate piece (11), a microscope body (2) capable of observing a sample, a slide box (3) assembled adjacent to the object stage (1), and a slide carrier (4) capable of placing a slide;
the microscope body (2) is suspended above the objective table (1);
the slide cassette (3) is provided with a cassette cavity capable of accommodating at least one slide carrier (4);
an opening (31) is formed in one end, adjacent to the object stage (1), of the box cavity;
the opening (31) faces to the plate part (11) of the objective table (1);
the slide carrier (4) is provided with at least two loading grooves (43) capable of placing slides, and the moving direction of the slide carrier in the slide box (3) is parallel to the moving direction of the slide carrier on the object stage (1);
a through hole (432) is formed in the loading groove (43);
a push-pull piece (7) capable of driving the slide carrier to translate is assembled on the objective table (1);
the push-pull piece (7) can adsorb or hook or stick or scarf joint the slide carrier;
a plurality of clamping grooves (32) for erecting slide carriers (4) are arranged in the cavity of the slide box (3);
the clamping grooves (32) protrude out of the inner cavity wall of the glass box (3) and are arranged in the glass box (3) in a bilateral symmetry mode.
2. A large flux microscope slide loading system as claimed in claim 1,
the push-pull piece (7) is connected with the plate piece (11) of the objective table (1) in a sliding way through a slide rail component (15);
the sliding rail component (15) is provided with a driving source which can drive the push-pull piece (7) to reciprocate on the panel piece (11).
3. A large flux microscope slide loading system as claimed in claim 1,
an I-shaped part (71) is arranged at one end of the push-pull piece (7) assembled with the slide carrier (4);
the slide carrier (4) is provided with a T-shaped hole (48) matched with the I-shaped part (71);
the I-shaped part can be embedded into the T-shaped hole (48) to realize the fixed connection of the push-pull piece (7) and the slide carrier (4) in the horizontal direction.
4. A large flux microscope slide loading system as claimed in claim 1,
the objective table (1) is connected with the microscope body (2) through a connector,
the lower end part of the focusing unit is connected with a focusing unit (14) which can drive the objective table (1) to reciprocate;
the focusing unit (14) is fixedly connected with a focusing knob of the microscope body (2);
the object stage (1) comprises a transverse moving component (12) capable of moving transversely and a longitudinal moving component (13) capable of moving longitudinally;
the transverse moving component (12) is assembled below the push-pull component (7), and the longitudinal moving component (13) is assembled below the transverse moving component (12) to realize transverse movement and longitudinal movement of the panel piece (11);
the objective table (1) is driven by the focusing unit (14) to move up and down to realize slide focusing.
5. A high flux microscope slide loading system according to any one of claims 1 to 4,
further comprising a cassette magazine (5) capable of holding cassettes (3);
the lower end part of the slide magazine (5) is provided with a lifting component which can drive the slide box (3) to lift.
6. A large flux microscope slide loading system as claimed in claim 5,
the lifting component is provided with a bottom plate for fixing the slide box (3);
one of the bottom plate and the glass box (3) is provided with a groove, and the other is provided with a bulge;
the projections can be embedded into the grooves, so that the glass box (3) is fixed on the lifting assembly.
7. A large flux microscope slide loading system as claimed in claim 6,
the two ends of the loading groove (43) are downwards sunken to form a longitudinally extending blocking part (431) for erecting a slide, the middle area of the loading groove is provided with the through hole (432), and a limiting part (42) capable of moving back and forth is arranged adjacent to the through hole (432);
at least one part of the limiting piece (42) is positioned in the through hole (432) and can move in the through hole (432) to change the loading space of the loading groove (43);
the limiting piece (42) is arranged on the slide carrier (4) through an elastic piece (44);
the elastic part (44) is a pressure spring, an air rod or a hydraulic rod, and the elastic part realizes the movement and reset of part or all of the limiting part (42) in the through hole (432);
one end of the limiting piece (42) adjacent to the slide is provided with an inverted trapezoidal surface structure (421), and the upper surface of the inverted trapezoidal surface structure is provided with a toggle key (422);
the upper end of the inverted stair surface structure (421) protrudes out of the upper surface of the loading groove (43), or is flush with the upper surface of the loading groove (43);
the toggle key (422) protrudes out of the upper surface of the loading groove (43);
the upper surface of the loading groove (43) is provided with a socket (411) for connecting a toggle key (422) in a penetrating way,
the size of the interface (411) is matched with the moving range of the toggle key (422).
8. A large flux microscope slide loading system as claimed in claim 7,
the lower end of the slide carrier (4) is provided with a plurality of sunken parts for accommodating the limiting parts (42);
the lower end of the depressed part is provided with a connecting strip (47) for covering a lower end opening (31) of the depressed part;
the connecting strip (47) is fixed on the slide carrier (4) and extends to the lower part of the limiting piece (42),
the upper wall surface of the connecting strip (47) and the lower wall surface of the slide carrier (4) form a mounting cavity for assembling the limiting piece (42).
9. A large flux microscope slide scanning method is characterized in that,
applying a high throughput microscope slide loading system as claimed in any one of claims 1 to 8;
it comprises the following contents:
the distance of the slide moving at each time is less than one window distance, so that the pictures shot at each time have an overlapped area;
then identifying the overlapping area of the slide pictures, and cutting or splicing the pictures to obtain a complete slide picture;
the method comprises the steps of pre-scanning a slide, preliminarily fitting a theoretical focusing position of the slide by shooting a plurality of points on the slide, and reducing the vertical axis movement distance when each point is shot subsequently.
10. A large flux microscope slide scanning method according to claim 9,
the method for shooting by adopting pre-scanning specifically comprises the following steps:
firstly, acquiring and checking information of a to-be-scanned film;
secondly, moving the object stage (1) to a preset position;
thirdly, the push-pull piece (7) moves to the upper part of the slide carrier (4);
fourthly, the slide box (3) drives the slide carrier (4) to move upwards, so that the I-shaped part of the push-pull piece (7) falls into a T-shaped hole (48) of the slide carrier (4);
fifthly, the push-pull piece (7) drives the slide carrier (4) and the slide to move to the lower part of the microscope body (2);
sixthly, adjusting the position of the microscope body (2) and prescanning a plurality of points on the slide;
and seventhly, performing coarse focusing according to the pre-scanning data: moving back to the objective lens;
eighthly, finding the clearest position of the rough focusing;
and ninthly, fine focusing is performed: moving towards the objective lens;
step ten, finding the clearest position of the fine focusing;
step ten, calculating the vertical moving position of the microscope body (2) according to the clearest fine focusing positions of the plurality of points which are pre-scanned;
and step twelve, the microscope body (2) switches the high power objective lens, moves to the focusing starting point and moves towards the end point, finds the clearest position, and takes a picture for storage.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011606108.7A CN112859316A (en) | 2020-12-30 | 2020-12-30 | Large-flux microscope slide loading system and scanning method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011606108.7A CN112859316A (en) | 2020-12-30 | 2020-12-30 | Large-flux microscope slide loading system and scanning method |
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| CN202011606108.7A Pending CN112859316A (en) | 2020-12-30 | 2020-12-30 | Large-flux microscope slide loading system and scanning method |
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Application publication date: 20210528 |