CN212610695U - Cell culture state remote observation mechanism - Google Patents
Cell culture state remote observation mechanism Download PDFInfo
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- CN212610695U CN212610695U CN202020922080.7U CN202020922080U CN212610695U CN 212610695 U CN212610695 U CN 212610695U CN 202020922080 U CN202020922080 U CN 202020922080U CN 212610695 U CN212610695 U CN 212610695U
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Abstract
The utility model provides a cell culture state remote observation mechanism, which comprises an observation platform, a microscope, a horizontal direction adjusting platform, a focusing device, an image acquisition device, a control unit and an intelligent terminal; the microscope is inversely arranged below the through hole of the observation platform through the lens bending cylinder, the culture dish is arranged on the horizontal direction adjustment and positioned above the through hole, and the focusing device is connected with the lens of the microscope and used for adjusting the focal length of the lens; the image acquisition device is connected with the microscope; the control unit is respectively connected with the horizontal direction adjusting platform, the focusing device, the image acquisition device and the intelligent terminal. The utility model discloses make the cell culture process not interrupted, can in time observe the cell simultaneously, can let operating personnel utilize intelligent terminal to realize observing the cultivation target, focus to the microscope, reduce operating personnel's work flow, improve the efficiency and the precision of observing, reduce the cultivation result by the artificial influence.
Description
Technical Field
The utility model belongs to the technical field of cell culture, concretely relates to cell culture state remote observation mechanism.
Background
Most cell culture equipment at present, operating personnel will open the incubator hatch door in an unscheduled period at the culture process, take out the culture dish, shift the cultivation target, and then observe, make the human analysis again, put back former incubator again, and then repeat preceding action, reach the target until cell growth. This operation flow is loaded down with trivial details, has reduced operating personnel's work efficiency, and can destroy the ideal growing environment of cell greatly, not only causes the cell growing environment that operating personnel wanted to see to suffer destruction, has also destroyed the growing environment of other cells in the incubator simultaneously. After the cells are taken out, the cells are conveyed to a microscope observation place in an environment unsuitable for cell growth, and the operation process can be finished through complicated processes such as focusing, observation, moving, observation, comparison and the like, but the process time is long, and the environment is only a common environment, so that unpredictable influence is caused on the cell growth. Meanwhile, different time and space concepts of different operators can influence the cell growth to different degrees, so that the consistency of cell culture is damaged, and the culture result of a culture target is influenced by deviating from an ideal state.
SUMMERY OF THE UTILITY MODEL
To the technical problem, the utility model provides a cell culture state remote observation mechanism for the cell culture process is not interrupted, can in time observe the cell simultaneously, carries out scientific record, can let operating personnel utilize intelligent terminal to realize observing the cultivation target, focuses on the microscope, reduces operating personnel's work flow, improves the efficiency and the precision of observing, reduces to cultivate the result by the artificial influence.
The utility model provides a technical scheme that its technical problem adopted is: a cell culture state remote observation mechanism comprises an observation platform, a microscope, a horizontal direction adjusting platform, a focusing device, an image acquisition device, a control unit and an intelligent terminal;
the observation platform, the microscope, the horizontal direction adjusting platform, the focusing device and the image acquisition device are all arranged in the cell culture box;
the microscope is inversely arranged below the through hole through the lens bending barrel, the horizontal direction adjusting platform is arranged on the observation platform, the culture dish is arranged on the horizontal direction adjusting platform and is positioned above the through hole, and the focusing device is connected with the lens of the microscope and is used for adjusting the focal length of the lens;
the image acquisition device is connected with the microscope and is used for acquiring an image of the culture dish observed by the microscope;
and the control unit is respectively connected with the horizontal direction adjusting platform, the focusing device, the image acquisition device and the intelligent terminal.
In the scheme, the device further comprises a manipulator; the manipulator is arranged in the cell culture box; the control unit is connected with the manipulator.
In the scheme, the device further comprises a culture dish storing and taking platform; the culture dish storing and taking platform is arranged in the cell culture box; the culture dish access platform is provided with a two-dimensional code reader, and the two-dimensional code reader is used for reading a two-dimensional code of the culture dish; the control unit is connected with the two-dimensional code reader.
In the above scheme, the horizontal direction adjusting platform comprises a first stepping motor module, a second stepping motor module, an upper table top, a middle table top and a horizontal direction adjusting platform bottom plate;
the upper table top, the middle table top and the bottom plate are sequentially overlapped from top to bottom, the upper table top is connected with the middle table top in a sliding manner, the middle table top is connected with the bottom plate of the horizontal direction adjusting platform in a sliding manner, and the bottom plate of the horizontal direction adjusting platform is connected with the observation platform; the middle parts of the upper table top, the middle table top and the horizontal direction adjusting platform bottom plate are provided with communicated through grooves, and the through grooves correspond to and are communicated with the through holes;
the first stepping motor module and the second stepping motor module are installed on the horizontal direction adjusting platform bottom plate, the first stepping motor module is connected with the upper table top and drives the upper table top to move along the X-axis direction, and the second stepping motor module is connected with the middle table top and drives the middle table top to move along the Y-axis direction.
Furthermore, the upper table top is connected with the middle table top through the first sliding block, and the middle table top is connected with the bottom plate through the rail.
In the above scheme, the focusing device comprises a motor, a coupler, a second slider, a screw rod and a focusing device bottom plate;
the motor, the coupler and the second sliding block are respectively arranged on a base plate of the focusing device; the motor is connected with the second sliding block through the coupler, one end of the screw rod is connected with the sliding block, the other end of the screw rod is connected with the objective lens, and the motor drives the second sliding block to slide and drive the objective lens to move along the Z-axis direction through the screw rod.
In the above scheme, the lens bending barrel comprises an objective lens interface, an eyepiece lens interface and a reflector;
the objective lens interface and the eyepiece lens interface are arranged vertically, the reflector is arranged at an angle of 45 degrees with the objective lens interface and the eyepiece lens interface respectively, and the reflector reflects light of the objective lens interface to the eyepiece lens interface.
In the above scheme, the intelligent terminal is a mobile terminal or a display screen.
In the above scheme, the device further comprises a lighting device; the lighting device is mounted above the observation platform.
Further, the lighting device is an LED lamp, and the LED lamp can adjust color and brightness.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses can realize that the cell culture process is not interrupted, guarantee to cultivate the growth of the no human interference of target, can in time observe the cell simultaneously, carry out scientific record, can let operating personnel utilize intelligent terminal to realize observing the cultivation target, focus to the microscope, reduce operating personnel's work flow, improve the precision of observing, reduce and cultivate the result by the artificial influence.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic view of a cell culture chamber according to an embodiment of the present invention;
FIG. 2 is a schematic view of a structure of a culture dish storing and taking platform according to an embodiment of the present invention;
fig. 3 is a schematic diagram of the operation of the robot according to an embodiment of the present invention;
fig. 4 is a schematic structural view of a horizontal direction adjustment platform according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a focusing apparatus according to an embodiment of the present invention, in which fig. 5 (a) is a top view, fig. 5 (b) is a side view, fig. 5 (c) is a front view, and fig. 5 (d) is a perspective view;
fig. 6 is a schematic structural view of a lens bending barrel according to an embodiment of the present invention.
In the figure: 1. an observation platform; 2. a cell incubator; 3. a horizontal direction adjustment platform; 301. a first stepping motor module; 302. a second stepping motor module; 303. an upper table top; 304. a middle table-board; 305. adjusting the platform bottom plate in the horizontal direction; 306. a first slider; 307, a track; 308. a through groove; 4. a manipulator; 5. a through hole; 7. a culture dish access platform; 8. a two-dimensional code reader; 9. a focusing device; 901. a motor; 902. a coupling; 903. a second slider; 904. a screw rod; 905. a coke device bottom plate; 10. a lens bending barrel; 1001. an objective lens interface; 1002. an eyepiece interface; 1003. a reflective mirror; 11. an illumination device; 12. a rack; 13. a separator.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "axial", "radial", "vertical", "horizontal", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
As shown in fig. 1, the cell culture state remote observation mechanism comprises an observation platform 1, a microscope, a horizontal direction adjustment platform 3, a focusing device 9, an image acquisition device, a control unit and an intelligent terminal; the observation platform 1, the microscope, the horizontal direction adjusting platform 3, the focusing device 9 and the image acquisition device are all arranged in the cell culture box 2.
The microscope is characterized in that a through hole 5 is formed in the observation platform 1, the microscope is inversely installed below the through hole 5 through a lens bending barrel 10, the horizontal direction adjusting platform 3 is installed on the observation platform 1, the culture dish is arranged on the horizontal direction adjusting platform 3 and located above the through hole 5, and the focusing device 9 is connected with the lens of the microscope and used for adjusting the focal length of the lens. Preferably, the microscope is a digital microscope, the lens of the microscope is upward, and the microscope can automatically move and focus the field of view through the horizontal direction adjusting platform 3 and the focusing device 9.
The image acquisition device is connected with the microscope and is used for acquiring images of the culture dish observed by the microscope. Preferably, the image acquisition device is an industrial camera.
The control unit is respectively connected with the horizontal direction adjusting platform 3, the focusing device 9, the image acquisition device and the intelligent terminal.
As shown in fig. 2 and 3, it is preferable according to the present embodiment to further include a culture dish access platform 7 and a manipulator 4; the culture dish storing and taking platform 7 and the manipulator 4 are both arranged in the cell culture box 2; the culture dish access platform 7 is provided with a two-dimensional code reader 8, and the two-dimensional code reader 8 is used for reading a two-dimensional code of the culture dish; the control unit is respectively connected with the two-dimensional code reader 8 and the manipulator 4. Two-dimensional code reader 8 reads culture dish bottom two-dimensional code information from the below to the direction, transmits the control unit and carries out the record processing, and the accuracy of accessible manipulator is snatched and is deposited, reduces to get and put the error, avoids appearing interfering the collision.
As shown in fig. 1 and 3, a plurality of racks 12 for storing culture dishes are arranged in the cell incubator 2, the racks 12, the culture dish storing and taking platform 7 and the observation platform 1 are arranged in a circular arc shape in the incubator body 1, and the manipulator 4 is installed at the center of the circular arc; the rack 12 is vertical to the bottom of the cell culture box 2; a plurality of rows of partition plates 13 for storing culture dishes are arranged on the rack 12. The partition plate 13 is provided with a detection device for detecting whether the culture dish is placed on the partition plate 13, each detection device is provided with different position codes in the control unit, and the detection devices are connected with the control unit. The manipulator 4 can be preferably a 6-shaft manipulator which is used as an actuating mechanism for taking and placing a culture dish, and the tail end finger clamps are opened and closed to clamp the upper surface and the lower surface of the culture dish.
As shown in fig. 4, according to the present embodiment, the horizontal direction adjustment platform 3 preferably includes a first stepping motor module 301, a second stepping motor module 302, an upper table 303, a middle table 304, a horizontal direction adjustment platform bottom plate 305, a first slide block 306 and a rail 307; the upper table top 303, the middle table top 304 and the horizontal direction adjusting platform bottom plate 305 are sequentially stacked from top to bottom, the upper table top 303 is connected with the middle table top 304 through a first sliding block 306, the middle table top 304 is connected with the horizontal direction adjusting platform bottom plate 305 through a rail 307, and the horizontal direction adjusting platform bottom plate 305 is connected with the observation platform 1; the middle parts of the upper table-board 303, the middle table-board 304 and the horizontal direction adjusting platform bottom board 305 are all provided with communicated through grooves 308, and the through grooves 308 correspond to and are communicated with the through holes 5; the first stepping motor module 301 and the second stepping motor module 302 are installed on a horizontal direction adjusting platform base plate 305, the first stepping motor module 301 is connected with an upper table board 303 and drives the upper table board 303 to move along the X-axis direction, and the second stepping motor module 302 is connected with a middle table board 304 and drives the middle table board 304 to move along the Y-axis direction.
As shown in fig. 5 (a), (b), (c), and (d), according to the present embodiment, the focusing device 9 preferably includes a motor 901, a coupling 902, a second slider 903, a lead screw 904, and a focusing device base plate 905; the motor 901, the coupling 902 and the second slider 903 are respectively arranged on a focusing device base plate 905; the focusing mechanism base plate 905 is connected to the cell culture chamber 2. The motor 901 is connected with the second slider 903 through a coupler 902, one end of the screw rod 904 is connected with the slider 903, the other end of the screw rod 904 is connected with the objective lens, and the motor 901 drives the second slider 903 to slide and drive the objective lens to move along the Z-axis direction through the screw rod 904.
As shown in fig. 6, according to the present embodiment, the lens barrel 10 preferably includes an objective lens interface 1001, an eyepiece lens interface 1002, and a mirror 1003; the objective lens interface 1001 and the eyepiece lens interface 1002 are arranged vertically, the reflective mirror 1003 and the objective lens interface 1001 and the eyepiece lens interface 1002 are arranged in an angle of 45 degrees respectively, and the reflective mirror 1003 reflects light of the objective lens interface 1001 to the eyepiece lens interface 1002. The lens bending barrel 10 adopts an integrated sealing design, saves space and objectively plays a role in dust prevention. Placing the modified microscope into the incubator 1, and taking pictures and recording videos of the culture target by using a configured industrial camera; the industrial camera can upload image data to the control unit through data transmission modes such as HDMI, USB and RS 485.
According to the embodiment, the intelligent terminal is a mobile terminal or a display screen.
Preferably according to the present embodiment, further comprises a lighting device 11; the lighting device 11 is mounted above the viewing platform 1. The lighting device 11 is an LED lamp which is lightened from top to bottom, is waterproof and dustproof and provides stable lumens; the LED lamp can also adjust color and brightness, and meets the observation requirements of different cell states.
According to this embodiment is preferred, the culture dish is put on culture dish access platform 7 earlier, and two-dimensional code reader 8 reads the two-dimensional code of culture dish to convey the control unit, the control unit control manipulator 4 presss from both sides and gets the culture dish and place appointed position, and the control unit passes through modes such as WIFI connection internet with data transfer to intelligent terminal, and intelligent terminal can be for mobile terminal such as cell-phone and can be the interactive display screen of human-computer. When the culture dish of assigned position is observed to needs, can control 4 clamps of manipulator through intelligent terminal and get the assigned culture dish, place observation platform 1, the cell proliferation state is upwards observed to the microscope, accessible horizontal direction adjusts platform 3 and adjusts the culture dish in the position of X axle and Y axle, adjusts the focus of objective through focusing device 9 to gather the cell state image through image acquisition device, transmit the control unit, the control unit with data transfer to intelligent terminal. The utility model discloses make the cell culture process not interrupted, guarantee to cultivate the growth of target without human interference, can in time observe the cell simultaneously, carry out scientific record, can let operating personnel utilize intelligent terminal to realize observing the cultivation target, focus to the microscope, can realize the observation of appointed culture dish through the control unit control manipulator, reduce operating personnel's work flow, improve the precision of observing, reduce the culture result by artificial influence.
The above detailed description is only for the purpose of illustrating the practical embodiments of the present invention, and they are not intended to limit the scope of the present invention, and all equivalent embodiments or modifications that do not depart from the technical spirit of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A cell culture state remote observation mechanism is characterized by comprising an observation platform (1), a microscope, a horizontal direction adjusting platform (3), a focusing device (9), an image acquisition device, a control unit and an intelligent terminal;
the observation platform (1), the microscope, the horizontal direction adjusting platform (3), the focusing device (9) and the image acquisition device are all arranged in the cell culture box (2);
the microscope is characterized in that a through hole (5) is formed in the observation platform (1), the microscope is inversely installed below the through hole (5) through a lens bending cylinder (10), the horizontal direction adjusting platform (3) is installed on the observation platform (1), a culture dish is arranged on the horizontal direction adjusting platform (3) and located above the through hole (5), and a focusing device (9) is connected with a lens of the microscope and used for adjusting the focal length of the lens;
the image acquisition device is connected with the microscope and is used for acquiring an image of the culture dish observed by the microscope;
the control unit is respectively connected with the horizontal direction adjusting platform (3), the focusing device (9), the image acquisition device and the intelligent terminal.
2. The cell culture state remote observation mechanism according to claim 1, further comprising a manipulator (4); the manipulator (4) is arranged in the cell culture box (2); the control unit is connected with the manipulator (4).
3. The cell culture state remote observation mechanism according to claim 1, further comprising a culture dish access platform (7); the culture dish storing and taking platform (7) is arranged in the cell culture box (2); the culture dish access platform (7) is provided with a two-dimensional code reader (8), and the two-dimensional code reader (8) is used for reading a two-dimensional code of a culture dish; the control unit is connected with the two-dimensional code reader (8).
4. The cell culture state remote observation mechanism according to claim 1, wherein the horizontal direction adjustment platform (3) comprises a first stepping motor module (301), a second stepping motor module (302), an upper table (303), a middle table (304) and a horizontal direction adjustment platform bottom plate (305);
the upper table top (303), the middle table top (304) and the horizontal direction adjusting platform bottom plate (305) are sequentially stacked and installed from top to bottom, the upper table top (303) is connected with the middle table top (304) in a sliding mode, the middle table top (304) is connected with the horizontal direction adjusting platform bottom plate (305) in a sliding mode, and the horizontal direction adjusting platform bottom plate (305) is connected with the observation platform (1); the middle parts of the upper table top (303), the middle table top (304) and the horizontal direction adjusting platform bottom plate (305) are respectively provided with a communicated through groove (308), and the through groove (308) corresponds to and is communicated with the through hole (5);
the first stepping motor module (301) and the second stepping motor module (302) are installed on a horizontal direction adjusting platform base plate (305), the first stepping motor module (301) is connected with an upper table board (303) to drive the upper table board (303) to move along the X-axis direction, the second stepping motor module (302) is connected with a middle table board (304), and the middle table board (304) is driven to move along the Y-axis direction.
5. The mechanism for remotely observing the state of cell culture according to claim 4, wherein said upper stage (303) is connected to a middle stage (304) by a first slide (306), and said middle stage (304) is connected to a horizontal direction adjustment stage base plate (305) by a rail (307).
6. The cell culture state remote observation mechanism according to claim 1, wherein the focusing means (9) comprises a motor (901), a coupling (902), a second slider (903), a lead screw (904), and a focusing means base plate (905);
the motor (901), the coupling (902) and the second sliding block (903) are respectively arranged on a focusing device base plate (905); the motor (901) is connected with the second sliding block (903) through a coupler (902), one end of the screw rod (904) is connected with the sliding block (903), the other end of the screw rod (904) is connected with the objective lens, and the motor (901) drives the second sliding block (903) to slide to drive the objective lens to move along the Z-axis direction through the screw rod (904).
7. The cell culture state remote observation mechanism according to claim 1, wherein the lens barrel (10) comprises an objective lens interface (1001), an eyepiece lens interface (1002), and a mirror (1003);
the objective lens interface (1001) and the eyepiece lens interface (1002) are vertically arranged, the reflector (1003) and the objective lens interface (1001) and the eyepiece lens interface (1002) are respectively arranged at an angle of 45 degrees, and the reflector (1003) reflects light of the objective lens interface (1001) to the eyepiece lens interface (1002).
8. The cell culture state remote observation mechanism of claim 1, wherein the intelligent terminal is a mobile terminal or a display screen.
9. The cell culture state remote observation mechanism according to claim 1, further comprising an illumination device (11); the lighting device (11) is arranged above the observation platform (1).
10. The cell culture state remote observation mechanism according to claim 9, wherein the illumination device (11) is an LED lamp, and the LED lamp is capable of adjusting color and brightness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020922080.7U CN212610695U (en) | 2020-05-27 | 2020-05-27 | Cell culture state remote observation mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020922080.7U CN212610695U (en) | 2020-05-27 | 2020-05-27 | Cell culture state remote observation mechanism |
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| Publication Number | Publication Date |
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| CN212610695U true CN212610695U (en) | 2021-02-26 |
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| CN202020922080.7U Active CN212610695U (en) | 2020-05-27 | 2020-05-27 | Cell culture state remote observation mechanism |
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Effective date of registration: 20230331 Address after: 210008 North side of the first floor of the Science and Technology Innovation Base, No. 3, Hengda Road, Nanjing Economic and Technological Development Zone, Nanjing, Jiangsu Province Patentee after: Nanjing Lambert Ax Biotechnology Co.,Ltd. Address before: 210046 room 107, unit 4, building 17, 108 Prince building west, Yaohua street, Qixia District, Nanjing City, Jiangsu Province Patentee before: Nanjing mingshengtong Electronic Technology Co.,Ltd. |