CN112841392A - Preparation method of perilla protein powder - Google Patents
Preparation method of perilla protein powder Download PDFInfo
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- CN112841392A CN112841392A CN202011599475.9A CN202011599475A CN112841392A CN 112841392 A CN112841392 A CN 112841392A CN 202011599475 A CN202011599475 A CN 202011599475A CN 112841392 A CN112841392 A CN 112841392A
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- 235000004347 Perilla Nutrition 0.000 title claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 29
- 239000000843 powder Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 244000124853 Perilla frutescens Species 0.000 title claims description 7
- 241000229722 Perilla <angiosperm> Species 0.000 claims abstract description 40
- 238000003825 pressing Methods 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 21
- 238000003916 acid precipitation Methods 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 238000001694 spray drying Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 239000003513 alkali Substances 0.000 claims abstract description 6
- 238000004090 dissolution Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000000839 emulsion Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 235000004348 Perilla frutescens Nutrition 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 230000001804 emulsifying effect Effects 0.000 claims description 5
- 239000012456 homogeneous solution Substances 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 239000008213 purified water Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000004945 emulsification Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 4
- 239000000725 suspension Substances 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 239000004744 fabric Substances 0.000 abstract description 2
- 239000012265 solid product Substances 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 19
- 241000196324 Embryophyta Species 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a preparation method of perilla protein powder, and relates to the technical field of deep processing of perilla. The specific method comprises seven steps of extraction alkali dissolution, centrifugation, acid precipitation, filter pressing, disintegration, adjustment homogenization and spray drying, and the invention has the advantages that: the method is suitable for preparing unshelled perilla seed protein, the acid-precipitated material enters a filter press for solid-liquid separation, Japanese MPP303SC high-density filter cloth is selected for filter pressing, solid products are reserved, the problems that perilla protein suspension cannot be subjected to effective centrifugal filtration and the like are solved, the investment is small, the capacity is high, and the method can be used for large-scale production.
Description
The technical field is as follows:
the invention relates to the technical field of deep processing of purple perilla, and particularly relates to a preparation method of purple perilla protein powder.
Background art:
perilla frutescens, perilla frutescens and perilla hemp, which are annual plants in the family of Labiatae, have a long history in China and are one of 60 articles issued by the Ministry of health, namely food and medicines. Perilla frutescens has wild and cultivated species distributed in China, Plumbum preparatium, Indonesia, Japan, Korea, etc. A large amount of plants are planted in the southwest, northwest and northeast of China as one of oil sources. The Heilongjiang province and surrounding areas have abundant perilla resources.
Perilla is an annual herbaceous plant. The planting application in China has about the history of about 2000 years, the planting is easy, and the yield is high. In recent years, perilla has become a multipurpose plant which is concerned by the world due to its unique active substances and nutritional ingredients, and has high economic value. Perilla frutescens is a plant with high utilization value for both medicine and food. The perilla seeds contain rich fat and protein, and a byproduct after the perilla seeds are pressed into oil, namely perilla meal, is rich in various nutrient substances, has high protein content and reasonable amino acid composition, and is a high-quality plant protein resource.
The prior art has the following disadvantages:
1. the ultrafiltration method has long production and processing time and needs a large amount of water for dilution and filtration. The investment cost of ultrafiltration equipment is high, the filter membrane is easy to block, forward and reverse flushing is carried out regularly in the production process, the ultrafiltration membrane has service life limitation, and the cost investment is increased when the filter membrane is replaced regularly;
2. the fructus Perillae is not peeled, and the obtained protein is dark and red brown. The shelled perilla protein suspension cannot be effectively centrifugally filtered and recovered;
3. the flocculation effect is poor after acid precipitation, protein suspension is not easy to separate, the centrifugal filtration effect is poor, and the recovery rate is low;
4. the ultrafiltration technology has small capacity and low production efficiency, and is not suitable for industrial large-scale production. Expanding the capacity requires a substantial increase in investment costs.
The invention content is as follows:
the invention aims to overcome the defects of the prior art and provides a preparation method of perilla protein powder.
The preparation method of perilla protein powder provided by the invention comprises the following operation steps:
(1) extraction alkali dissolution: when 600kg of perilla defatted powder is added to 9m3Purified water, pH value is adjusted to 9.0 +/-0.2 at 50 ℃, and heat preservation extraction is carried out for 2 hours to obtain extract liquor;
(2) centrifuging: centrifuging the extract obtained in the step (1) at the rotating speed of 6500rpm to obtain a centrifugate;
(3) acid precipitation: regulating the pH value of the centrifugate obtained in the step (2) to 4-5 at the temperature of 85 +/-2 ℃, and preserving the temperature for 1h to obtain an acid precipitation mixed solution;
(4) and (3) filter pressing: carrying out filter pressing on the acid precipitation mixed liquid prepared in the step (3) by a filter press, and drying by compressed air to obtain a filter pressing material;
(5) breaking: mixing and emulsifying the filter-pressed material obtained in the step (4) with water to obtain an emulsion; circularly crushing the emulsion for 15-35 min by using a crusher to obtain crushed solution;
(6) callback and homogenizing: adjusting the pH of the disintegration solution obtained in the step (5) to 6.0-6.5 by using 30% sodium hydroxide, stirring for 10min, and homogenizing under the pressure condition of 20mpa +/-2 mpa to obtain a homogeneous solution;
(7) spray drying: and (4) sterilizing the homogenized liquid obtained in the step (6) at the temperature of 120 +/-2 ℃, and then carrying out spray drying at the temperature of 160 +/-10 ℃ to obtain the perilla protein powder.
As a further improvement of the invention, in the step (1), 0.05-0.08% of sodium hydroxide is used for adjusting the pH.
As a further improvement of the invention, in the step (2), first-stage separation is carried out by a horizontal centrifuge, and then second-stage separation is carried out by a butterfly centrifuge.
As a further improvement of the invention, hydrochloric acid aqueous solution with the volume ratio of 1: 1-3 is dripped in the step (3) to adjust the pH.
As a further improvement of the invention, the emulsification is carried out after the pH value is adjusted to be 6.5 +/-0.1 by adding water in the step (5).
Wherein the perilla defatted powder is solid and is obtained by performing supercritical extraction on perilla seed meal, and is obtained by introducing carbon dioxide into perilla seed meal and performing solid-liquid separation in high-pressure extraction equipment; the selected horizontal centrifuge is LW 580/2500-N horizontal centrifuge produced by Dazhongda centrifugal machinery Limited company in the tin-free market; the butterfly centrifuge is a DHN500 disc centrifuge produced by Jiangsu giant energy machinery Limited company, and the filter press is MQLY25m produced by Jiangsu Meiqing environmental protection equipment engineering technology Limited company2And (4) a filter press.
The invention has the advantages that: the method is suitable for preparing unshelled perilla seed protein, the acid-precipitated material enters a filter press for solid-liquid separation, Japanese MPP303SC high-density filter cloth is selected for filter pressing, solid products are reserved, the problems that perilla protein suspension cannot be subjected to effective centrifugal filtration and the like are solved, the investment is small, the capacity is high, and the method can be used for large-scale production.
The specific implementation mode is as follows:
example 1
A preparation method of perilla protein powder comprises the following operation steps:
(1) extraction alkali dissolution: when 600kg of perilla defatted powder is added to 9m3Adjusting the pH of purified water to 9.0 +/-0.2 by using 0.05-0.08% of sodium hydroxide at 50 ℃, and performing heat preservation extraction for 2 hours to obtain an extract liquid; (ii) a
(2) Centrifuging: centrifuging the extract obtained in the step (1) at the rotating speed of 6500rpm, firstly using a horizontal centrifuge, and performing secondary separation on the extract by using a butterfly centrifuge to obtain the extract with the protein content of more than 80%;
(3) acid precipitation: dropwise adding a hydrochloric acid aqueous solution with a volume ratio of 1: 1-3 into the centrifugate obtained in the step (2) at a temperature of 85 +/-2 ℃, adjusting the pH value of the solution to 4.5 +/-0.1, and preserving heat for 1h to obtain an acid precipitation mixed solution;
(4) and (3) filter pressing: carrying out filter pressing on the acid precipitation mixed liquid prepared in the step (3) by a filter press, and drying by compressed air to obtain a filter pressing material;
(5) breaking: adjusting the filter-pressed material obtained in the step (4) and water to be neutral, wherein the pH value is 6.5 +/-0.1, and then mixing and emulsifying to better maintain the material properties to obtain an emulsion; circularly crushing the emulsion for 15-35 min by using a crusher to obtain crushed solution; circulating crushing operation is carried out for 15min each time the filter pressing material enters the crusher, and circulating crushing is carried out for 20min when the last filter pressing material enters the crusher;
(6) callback and homogenizing: adjusting the pH of the disintegration solution obtained in the step (5) to 6.0-6.5 by using 30% sodium hydroxide, stirring for 10min, and homogenizing under the pressure condition of 20mpa +/-2 mpa to obtain a homogeneous solution;
(7) spray drying: and (4) sterilizing the homogenized liquid obtained in the step (6) at the temperature of 120 +/-2 ℃, and then carrying out spray drying at the temperature of 160 +/-10 ℃ to obtain the perilla protein powder.
Example 2
A preparation method of perilla protein powder comprises the following operation steps:
(1) extraction alkali dissolution: when 600kg of perilla defatted powder is added to 9m3Purified water, sodium hydroxide with the temperature of 50 ℃ and the concentration of 0.05-0.08 percent is used for adjusting the pH value to 9.0 +/-0.2,extracting for 2h under heat preservation to obtain extract liquor; (ii) a
(2) Centrifuging: centrifuging the extract obtained in the step (1) at the rotating speed of 6500rpm, firstly using a horizontal centrifuge, and performing secondary separation on the extract by using a butterfly centrifuge to obtain the extract with the protein content of more than 80%;
(3) acid precipitation: dropwise adding a hydrochloric acid aqueous solution with a volume ratio of 1: 1-3 into the centrifugate obtained in the step (2) at a temperature of 85 +/-2 ℃, adjusting the pH value of the solution to 4.5 +/-0.1, and preserving heat for 1h to obtain an acid precipitation mixed solution;
(4) and (3) filter pressing: carrying out filter pressing on the acid precipitation mixed liquid prepared in the step (3) by a filter press, and drying by compressed air to obtain a filter pressing material;
(5) breaking: adjusting the filter-pressed material obtained in the step (4) and water to be neutral, wherein the pH value is 6.5 +/-0.1, and then mixing and emulsifying to better maintain the material properties to obtain an emulsion; circularly crushing the emulsion for 15-35 min by using a crusher to obtain crushed solution; circulating crushing operation is carried out for 15min each time the filter pressing material enters the crusher, and circulating crushing is carried out for 20min when the last filter pressing material enters the crusher;
(6) callback and homogenizing: adjusting the pH of the disintegration solution obtained in the step (5) to 6.0-6.5 by using 30% sodium hydroxide, stirring for 10min, and homogenizing under the pressure condition of 20mpa +/-2 mpa to obtain a homogeneous solution;
(7) spray drying: and (4) sterilizing the homogenized liquid obtained in the step (6) at the temperature of 120 +/-2 ℃, and then carrying out spray drying at the temperature of 160 +/-10 ℃ to obtain the perilla protein powder.
Example 3
A preparation method of perilla protein powder comprises the following operation steps:
(1) extraction alkali dissolution: when 600kg of perilla defatted powder is added to 9m3Adjusting the pH of purified water to 9.0 +/-0.2 by using 0.05-0.08% of sodium hydroxide at 50 ℃, and performing heat preservation extraction for 2 hours to obtain an extract liquid; (ii) a
(2) Centrifuging: centrifuging the extract obtained in the step (1) at the rotating speed of 6500rpm, firstly using a horizontal centrifuge, and performing secondary separation on the extract by using a butterfly centrifuge to obtain the extract with the protein content of more than 80%;
(3) acid precipitation: dropwise adding a hydrochloric acid aqueous solution with a volume ratio of 1: 1-3 into the centrifugate obtained in the step (2) at a temperature of 85 +/-2 ℃, adjusting the pH value of the solution to 4.5 +/-0.1, and preserving heat for 1h to obtain an acid precipitation mixed solution;
(4) and (3) filter pressing: carrying out filter pressing on the acid precipitation mixed liquid prepared in the step (3) by a filter press, and drying by compressed air to obtain a filter pressing material;
(5) breaking: adjusting the filter-pressed material obtained in the step (4) and water to be neutral, wherein the pH value is 6.5 +/-0.1, and then mixing and emulsifying to better maintain the material properties to obtain an emulsion; circularly crushing the emulsion for 15-35 min by using a crusher to obtain crushed solution; circulating crushing operation is carried out for 15min each time the filter pressing material enters the crusher, and circulating crushing is carried out for 20min when the last filter pressing material enters the crusher;
(6) callback and homogenizing: adjusting the pH of the disintegration solution obtained in the step (5) to 6.0-6.5 by using 30% sodium hydroxide, stirring for 10min, and homogenizing under the pressure condition of 20mpa +/-2 mpa to obtain a homogeneous solution;
(7) spray drying: and (4) sterilizing the homogenized liquid obtained in the step (6) at the temperature of 120 +/-2 ℃, and then carrying out spray drying at the temperature of 160 +/-10 ℃ to obtain the perilla protein powder.
The following table 1 is the quality standard of the finished perilla protein powder:
TABLE 1 quality Standard of the finished Perilla protein powder
Claims (5)
1. The preparation method of perilla frutescens protein powder is characterized by comprising the following operation steps:
(1) extraction alkali dissolution: when 600kg of perilla defatted powder is added to 9m3Purified water, pH value is adjusted to 9.0 +/-0.2 at 50 ℃, and heat preservation extraction is carried out for 2 hours to obtain extract liquor;
(2) centrifuging: centrifuging the extract obtained in the step (1) at the rotating speed of 6500rpm to obtain a centrifugate;
(3) acid precipitation: regulating the pH value of the centrifugate obtained in the step (2) to 4-5 at the temperature of 85 +/-2 ℃, and preserving the temperature for 1h to obtain an acid precipitation mixed solution;
(4) and (3) filter pressing: carrying out filter pressing on the acid precipitation mixed liquid prepared in the step (3) by a filter press, and drying by compressed air to obtain a filter pressing material;
(5) breaking: mixing and emulsifying the filter-pressed material obtained in the step (4) with water to obtain an emulsion; circularly crushing the emulsion for 15-35 min by using a crusher to obtain crushed solution;
(6) callback and homogenizing: adjusting the pH of the disintegration solution obtained in the step (5) to 6.0-6.5 by using 30% sodium hydroxide, stirring for 10min, and homogenizing under the pressure condition of 20mpa +/-2 mpa to obtain a homogeneous solution;
(7) spray drying: and (4) sterilizing the homogenized liquid obtained in the step (6) at the temperature of 120 +/-2 ℃, and then carrying out spray drying at the temperature of 160 +/-10 ℃ to obtain the perilla protein powder.
2. The preparation method of perilla protein powder according to claim 1, wherein the pH is adjusted by using 0.05-0.08% sodium hydroxide in the step (1).
3. The preparation method of perilla protein powder according to claim 1, wherein the step (2) comprises first-stage separation by a horizontal centrifuge and then second-stage separation by a butterfly centrifuge.
4. The preparation method of perilla frutescens protein powder as claimed in claim 1, wherein hydrochloric acid aqueous solution with a volume ratio of 1: 1-3 is added dropwise in the step (3) to adjust the pH.
5. The method for preparing perilla protein powder according to claim 1, wherein the emulsification is performed after the ph value is adjusted to 6.5 ± 0.1 by adding water in the step (5).
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| CN202011599475.9A CN112841392A (en) | 2020-12-29 | 2020-12-29 | Preparation method of perilla protein powder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011599475.9A CN112841392A (en) | 2020-12-29 | 2020-12-29 | Preparation method of perilla protein powder |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4147785A1 (en) * | 2021-09-10 | 2023-03-15 | Alfa Laval Corporate AB | Method of concentrating a plant-based protein suspension |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023036660A1 (en) * | 2021-09-10 | 2023-03-16 | Alfa Laval Corporate Ab | Method of concentrating a plant-based protein suspension |
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