CN1128213C - Fungal strain and method for preventing and killing weeds - Google Patents

Abstract

The present invention relates to a fungus strain and a method for biologically preventing and killing weeds, which belongs to the field of microbe application on agricultural plant protection. The strain (QZ-2000) is preserved with the number of CGMCC NO. 0640 by China Microbe Strain Preservation Committee in October 8, 2001. Mycelium with the conidiospores of the strain has the concentration of 3 to 50 g/L, or the conidiospores of the strain are matched with surfactants or adjuvants (such as tween, lecithin, vegetable oil, sodium alginate, N ketone, small 4204, etc.) to be safely used for biologically killing the weeds in corn fields, soybean fields, cotton fields, peanut fields, vegetable fields and orchards without pollution. Simultaneously, weeds, such as crab weeds, green bristle weeds, barnyard weeds, etc., can be controlled and a control effect on target weeds can reach more than 85%. The strain can also be matched with other strains controlling broadleaf weeds to simultaneously control a plurality of kinds of weeds and can also by matched with chemical herbicide to control the weeds.

Description

A kind of fungal bacterial strain and be used for the method for management of weeds

The present invention is a kind of fungal bacterial strain and the method that is used for management of weeds thereof; belong to the technical field that microorganism is applied to agriculture plant protection, prevents and kill off farmland weed, be exclusively used in preventing and kill off of farmland weed lady's-grass (Digitaria sp.) and barnyard grass grass (Echinochloa sp.) and Herba Setariae Viridis (Setaria sp.).

Along with people are residual to chemical herbicide, the wideling popularize of understanding, green food and the Organic farming of environmental pollution and resistance and the development of resistance weeds, development mycoherbicide management of weeds has become and has produced actual active demand; Also be an emerging technology and the most active research field of weed control.It has less investment, the lead time is short, specificity is strong, environmentally safe and do not produce advantages such as pollution.The substitute products that are considered to following chemical herbicide.At present, business-like campelyco kind has DeVine andCollego that develops the beginning of the eighties and the Comperico and Biochon that releases the end of the nineties.These weedicides have played vital role on the relative target weeds of control, or even unique selection.Long-lasting as DeVine can guarantee to use once, and 2-3 is weeding again.Being hopeful business-like campelyco item 0 has tens of, and the campelyco kind of wherein preventing and kill off weeds such as barnyard grass grass, wild water chestnut has very tempting prospect.

Lady's-grass is the malignant weed in autumn maturing Dry crop fields such as corn, Chinese sorghum, soybean, peanut, and is serious to the harm of growth of seedling such as cotton, tobacco, crudefiber crop, sugarcane, also is the advantage weeds in vegetables field, orchard, lawn and garden crop field.In China, about about 6,000 ten thousand hectares of the hazard area of lady's-grass accounts for the over half of autumn maturing Dry crop field weed total losses by its crop that causes loss.The distribution range of lady's-grass is extensive, is the torrid zone, the world and area, temperate zone cosmopolitan species.In the U.S., lady's-grass causes value of farm production loss 10%-20% every year, and annual in recent years loss is up to 20,000,000,000 dollars.This weeds fecundity is strong, fast growth, and vegetative period is long, consumes soil fertility, shading, resistance is strong, and artificial, machinery is prevented and kill off the comparison difficulty.Though chemical control is effective,, the use of chemical herbicide causes that the generation of resistant weed and serious environmental pollute, and serious causes land deterioration.The development campelyco is prevented and kill off lady's-grass and not only can effectively be prevented and kill off this weeds, and environmentally safe property height.At present, about the research of lady's-grass mycoherbicide, the U.S. has applied for that the patent No. is 5,635,444 and 5,952,264 with the patent of Curvularia (Curvularia) bacterial strain biological control lady's-grass.Its employed technology is proposed in this patent documentation, production method is safer to soybean, cotton, and is but not mentioned to corn and rice safety and use thereof.Test this patented strain through us and also infect corn and paddy rice.Still there is not other fungi to prevent and kill off the campelyco report of lady's-grass both at home and abroad.The present invention utilizes the mould genus of hyperbolic spore (Nakataea) bacterial strain development campelyco control lady's-grass, and this bacterial strain is safe to staple crops, applied range, thereby more superior than the patent of the U.S..This patent will have very significant prospect and social, economic benefit.

The objective of the invention is malignant weed lady's-grass at old wives' summer Dry crop fields such as corn, soybean and vegetables field, orchard, lawn and garden crop field, screen a kind of fungal bacterial strain, be used for the biocontrol crop smothering, can control and prevent and treat malignant weed safely and effectively, especially lady's-grass weeds, cost is low, and is pollution-free, to farm crop safety.

The present invention is a kind of fungal bacterial strain and the method that is used for management of weeds thereof, and its content and embodiment are as follows:

A kind of fungal bacterial strain QZ-2000, (depositary institution: China Microbial Culture Preservation Commission; Preservation date: October 8 calendar year 2001, preserving number: CGMCC NO.0640).A kind of fungal bacterial strain QZ-2000, imperfect fungi (Fungi Imperfecti), Moniliales (Moniliales), Moniliaceae (Moniliaceae), the mould genus of hyperbolic spore (Nakataea Hara), the bacterium colony of QZ-2000 is olive colour or blackish green on the PDA substratum; The conidiophore list is given birth to or grown thickly, and upright, olive brown or black have barrier film, and is 48～196 * 4～6 microns, single or once in a while at the top branch, elongated; Conidium forms continuously, the shoot top that new spore life is born below previous spore, and the spore size is 30～36 * 5～21 microns, fusiformis, little curved or curved to a side, two terminal cells are little and round sharp, colourless or light color, two cells in middle part are big, and color depth produces four oily balls sometimes.The sclerotium sphere.

The method that the mould genus bacterial strain of above-mentioned hyperbolic spore QZ-2000 is used for management of weeds is:

With concentration is that conidium or the mycelia of the mould genus bacterial strain of the hyperbolic spore QZ-2000 of 3-50g/L carries out foliage-spray or seed treatment to weeds.

The conidium of this bacterium can be used with all kinds of auxiliarys; Perhaps be used with the conidium of this bacterium and the chemical herbicide of 1/8 to 1/20 conventional amount used; Perhaps unite use with other bacterial parasite that is separated to from lady's-grass with the conidium of this bacterium; Perhaps use conidium and glue born of the same parents anthrax-bacilus veronica specialized form (Colletotrichum gloeosporioides f.sp.veronicae Sheng Qiang et Qing Zeng), the Alternaria zinniae (Alternaria zinniae) of this bacterium to unite use and prevent and kill off farm crop seedling phase weeds lady's-grass, barnyard grass grass, Herba Setariae Viridis, veronica, Tender Catchweed Bedstraw Herb, Siberian cockleburs etc. such as cotton, soybean, corn, peanut simultaneously.

Auxiliary refers to mainly that alginic acid is received, tween, vegetables oil, Yelkin TTS, glucose, starch, N ketone, little by 4204, N fertilizer; Above-mentioned chemical herbicide mainly refer to acetochlor, alachlor, metolachlor, propisochlor, naftypramide, isoproturon, chlorotoluron, sencorex, oxadiazon, Diuron Tech if so, Yu Nongle, take pounce on clean, cover grass energy, high cover grass energy, quizalofopethyl, smart quizalofopethyl, quizalofop, quizalofopPethyl, surely kill, fluazifop-p-butyl, fenoxaprop-P, fenoxapropPethyl, bentazone, make that it is grand, 2 first, 4 chlorine, Dicamba, prowers, weeds burn, restrain wealthy pleasure; Above-mentioned other bacterial parasite mainly refers to Magnaporthe grisea (Pyricularia Sacc), curved spore mould (Curvularia), chain lattice spore (Alternaria), long wriggle spore (Helminthosporium), Fusarium (Fusarium).Its method that is used all is the routine techniques that those skilled in the art grasped.

The contriver has found the natural occurrence phenomenon of field lady's-grass in the biological control research process to lady's-grass, so its parasitical fungi is carried out screening study, found that and separate a kind of bacterial strain obtain crop and other most of plant safety, but lady's-grass is had very strong pathogenicity rate, and the control effect after the week is more than 85%.In addition, also infect barnyard grass grass and Herba Setariae Viridis.The culture condition of microbial inoculum, production in enormous quantities method have all been carried out detailed research.The infection of this bacterium the condition of causing a disease has also been carried out particular study work.Demonstrating this bacterium has bigger potential development to become the commercialization campelyco.Can be used for most of crops field control weeds lady's-grass and barnyard grass grass and Herba Setariae Viridis.

Turning white in the middle of purple or the red-purple scab that the lady's-grass that the present invention collects is fallen ill and all as seen differed in size on the plant leaf, scab, is purple or red-purple on every side.Scab just is a round point shape, after expand as ovally or irregularly shaped, a plurality of scabs associatings cause the whole necrosis of blade when serious.The mycelia that the visible thickness of the sick leaf of observation does not wait under light microscopic stretches out from blade, is also shown in the conidium of fragmentary distribution.Obtain 6 kinds of fungies after separating, its cultural characters and pathogenic feature are as follows:

Through observing, the bacterium colony of QZ-2000 is olive colour or blackish green on the PDA substratum; The conidiophore list is given birth to or grown thickly, and upright, olive brown or black have barrier film, and is 48～196 * 4～6 microns, single or once in a while at the top branch, elongated; Conidium forms continuously, the new skill top that new spore life is born below previous spore, and the spore size is 30～36 * 5～21 microns, fusiformis, little curved or curved to a side, two terminal cells are little and round sharp, colourless or light color, two cells in middle part are big, and color depth produces four oily balls sometimes.The sick leaf of the lady's-grass that nature is adopted back illness from bottom to top increases the weight of gradually, top blade has many oval red-purple scabs, the bottom leaf is then large stretch of mashed dead to the full wafer yellow, under certain humidity condition, the mould layer of visible many black in surface or beige is done the visible a large amount of conidiums identical with culture condition of disease leaf microscopy.

The pathogenic symptom of spore on lady's-grass that the mould genus bacterial strain of hyperbolic spore QZ-2000 pure culture produces is: the red-purple scab appears in blade at first, round point shape, and quantity increases like numb spot subsequently, posterior lobe sheet or full wafer death.If the leaf heart is infected, then cause the very fast death of whole plant.The mould layer of visible black color or brown on the sick leaf.Through observing, the spore of sick leaf surface is the same with the mould genus spore of the hyperbolic spore that is sprayed.

Disease symptom under the above experiment condition is similar to natural illness.

By its characterized be: imperfect fungi (Fungi Imperfecti), Moniliales (Moniliales), Moniliaceae (Moniliaceae), the mould genus of hyperbolic spore (Nakataea Hara).This bacterium is mainly infected knotgrass plant and barnyard grass grass, Herba Setariae Viridis, to crop immunity such as paddy rice, corn, cotton, wheat, soybean, peanut, cowpeas, to broad leaf crop and weeds immunity, so can be used for above crop field controlling weeds.

The QZ-2000 cultural method is: available following culture medium culturing is also induced the generation conidium:

PDA substratum---lady's-grass soaks the juice nutrient agar---

Potato 200g lady's-grass cauline leaf 60g (clean and dry, pulverize, filter)

Glucose 20g agar 10g

Agar 20g distilled water 1L

Water 1L

MDYEA substratum-lady's-grass soaks juice-PDA substratum---

Semen Maydis powder 20g lady's-grass cauline leaf 60g (clean and dry, pulverize, filter)

Glucose 20g potato 200g

Yeast extract 2g glucose 20g

Agar 20g agar 10g

Distilled water 1L water 1L

CDA substratum-MSSA substratum---

NaNO3 2g Semen Maydis powder 15g

K ₂HPO4 1g analysis for soybean powder 15g

KCL 0.5g sucrose 15g

MgSO4 0.5g agar 20g

FeSO4 0.01g water 1L

Sucrose 30g MMA substratum---

Agar 10g Semen Maydis powder 20g

Water 1L agar 20g

Water 1L

The PCA substratum---

Potato 20g (boil 1 hour, filter) with dusting cover

Radix Dauci Sativae 20g (boil 1 hour, filter) with dusting cover

Agar 20g

Water 1L Semen Maydis powder-bean powder-liquid sucrose substratum is pressed Semen Maydis powder bean powder sucrose solids culture medium prescription, but removes agar.

The production in enormous quantities of this bacterium can be adopted solid or liquid-solid associating cultural method.Solid culture method: QZ-2000 is inoculated in the culture dish that the above-mentioned any solid medium of 15ml is housed, add a cover under the ultraviolet lamp that places 20 watts, shine 12h every day and cultivate (lamp is apart from culture dish 20cm), cultivate after 8 days, wash spore with sterilized water and promptly obtain spore liquid.Or collect spore with rotatory evaporator and place CaCl ₂Be drying to obtain xerospore powder (wettable powder) in the moisture eliminator.Liquid-solid cultural method: the above-mentioned bacterium colony fritter of cultivating through solid medium was inoculated in Semen Maydis powder-bean powder-liquid sucrose substratum shaking culture 6 days, after raffinate is cultivated in elimination, with aseptic water washing three times, obtain mycelium, mycelium can be directly used in production, also can be placed on the electrical grinding machine and pulverize, continue 60 seconds, promptly obtain mycelium fragment suspension, be poured on again in pan or the culture dish, thickness is about 1mm, places (apart from 20cm) Continuous irradiation 36h under 40 watts of fluorescent lamps, gets final product to such an extent that spore is used for producing.

The present invention compared with prior art, its major advantage and positively effect are as follows:

1. use the mould microbial inoculum of lady's-grass hyperbolic spore of the present invention to be used for management of weeds, not only can prevent and kill off soybean, cotton dicotyledonous crops field piece weeds, safer to soybean, cotton, and to corn and rice safety, can prevent and kill off corn and paddy rice monocot crops field piece weeds, be better than United States Patent (USP) of the prior art.

2. use the mould microbial inoculum of lady's-grass hyperbolic spore of the present invention to be used for management of weeds, especially can murder monocotyledon weed, be better than the chemical herbicide of conventional anti-monocotyledon weed, almost can not between monocot crops and weeds, select at monocot crops field piece.Can relievedly use in the broad leaf crop field in addition.Show that at its host range testing research this bacterium to the seedlings of crops such as wheat, soybean, cotton, cowpea, Sunflower Receptacle, corn, peanut, paddy rice safety all, only has slight infection to weeds Herba Setariae Viridis, barnyard grass grass.The host range of this bacterium is single-minded relatively, is safe to agriculture production.(seeing Table 1)

3. the present invention uses biological weed control, be utilize to live biological itself, come from employed environment, its degraded product is organism capable of circulation, can be because of use does not cause polluting, thereby, environmentally safe, can be used for producing green or organic farm products.

4. be used under wet condition or with relevant auxiliary agent, this bacterium can reach 85%-100% to target weeds (lady's-grass, barnyard grass grass, Herba Setariae Viridis) control effect, particularly the following plant of 3 leaf ages.(seeing Table 2)

The host range of the mould genus bacterial strain of table 1 lady's-grass hyperbolic spore QZ-2000 is measured

Section's name kind life susceptible spore concentration of treatment time

Degree (g/L) Herba Commelinae Commelinacea Herba Commelinae Commelina communis 2 leaf NS 10

Match end Commelina bengalensis 2 leaf NS 10 Malvaceae Malvaceae cotton Gossypium hirsutum 2.0-5 leaf NS 10 composite family Compositae Sunflower Receptacle Helianthus annus 2 leaf NS 10 pulse family Leguminosae soybean Glycine max 2.0-5.0 leaf NS 10

Cowpea Vigna sinensis 2 leaf NS 10

Peanut Arachis hypogaea 2 leaf NS 10 Gramineae Gramineae corn Zea mays 1 leaf NS 10

Wheat Triticum aestivum 1 leaf NS 10

Paddy rice Oryza sativa 2 leaf NS 10

Lady's-grass Digitaria sanguinalis 1.0-3.5 leaf SS 10

Barnyard grass grass Echinochloa crusgalli 1.0-3.5 leaf SS 10

Herba Setariae Viridis Setaria viridis 1.0-2.0 leaf SS 10 annotates: NS represents not react SS and represents seriously susceptible

Table 2 lady's-grass leaf age is to the influence of the mould genus bacterial strain of hyperbolic spore QZ-2000 virulence

One Tuesday week the plant leaf age

The susceptible rate of the susceptible rate of disease index (%) (%) disease index (%) (%) 0.8 leaf is handled 100AaEe 100 100Aa Ee 100

CK 0BbHi 0 0BbJi 01.5 leaf is handled 98.3 ± 0.15AaEe, 100 92.6 ± 0.22AaEf 100

CK 0BbHi 0 0BbJi 02.5 leaf is handled 85.9 ± 0.57Aa Ff, 100 82.49 ± 0.47AaFf 100

CK 0BbHi 0 0.29 ± 0.05BbJi 53.5 leaves are handled 80.5 ± 0.85Aa Ff, 100 78.3 ± 0.64AaFGg 100

CK 0.22 ± 0.02BbHi 0 6.5 ± 0.34BbHJi 94.5 leaves are handled 65 ± 0.63Aa Gg, 100 60.07 ± 1.06AaGb 100

(A-B is the significance of difference between handling and contrasting to CK 4.1 ± 0.15BbHh 0 11.3 ± 0.5BbHi 15; E-J is the significance of difference that whole diseases refer to)

Embodiment 1: area lady's-grass disease plant is gathered and isolates several fungies from spontaneous generation in Nanjing, and the pathogenic of them tested.

Sick leaf from the field acquisition to the lady's-grass, record plant disease symptom, microscopy.Carry out the separation of pathogenic fungi with lady's-grass juice substratum.At the monospore of each bacterial strain of microscopically picking, be seeded in respectively on the PDA substratum, in dark, 28 ℃ of cultivations down, observe the colony characteristics of pathogenic fungi pure culture.Each bacterium colony disk of cut-off footpath 6mm is seeded in the dull and stereotyped central authorities of PDA in addition, measures each colony diameter after 7 days.(table 3)

Various pathogenic fungi spore suspensions with different concns spray lady's-grass respectively, observe the pathogenic property (table 4) of each fungi.

The comparison of table 3 lady's-grass pathogenic fungi colony characteristics

Bacterium colony

Strain identification is D (mm) as a result

Thickness color surface strain QZ-200 0 hyperbolic spore mould 8.5 ++ the cotton-shaped strain QZ-200 2 curved spore moulds 8.9 of the thick cotton-shaped strain QZ-200 1 Magnaporthe grisea 4.2+celadon of+dark olive+blackish green powdery strain QZ-200 3 long spore bacterium 9.0 that wriggle ++ blue cotton-shaped strain QZ-200 4 alternaric bacterias 9.0 ++ thick cotton-shaped strain QZ-200 5 Fusariums 8.3 of+canescence ++ the lady's-grass disease fungus that faint yellow thick cotton-shaped D represents colony diameter table 4 variable concentrations that forms behind the 6mm bacterium colony disk 7d is pathogenic to 1.5-3.5 Leaf-Age-Period lady's-grass

Embodiment 2: the QZ-2000 bacterial strain was cultivated 7 days with ultra violet lamp (12 hours/day) on the PDA substratum, scraped media surface gently with slide, by filtered through gauze, collect filtrate and microscopy spore concentration (10g/L) with aseptic water washing; Spray filtrate with manual sprayer, be inoculated on 1 leaf, 1 heart to 3 leaf, the lady's-grass of 1 heart stage, preserved moisture 12 hours.After handling five days, this careless preventive effect is reached more than 90%.Embodiment 3: press the method for embodiment 2, making concentration is the mould genus bacterial strain of hyperbolic spore QZ-2000, bacterial strain 2001, bacterial strain 2002, the bacterial strain 2003 of 10g/L, more isopyknic various spore suspensions is mixed by following prescription; F ₁: bacterial strain QZ-2000+ bacterial strain 2001; F ₂: bacterial strain QZ-2000+ bacterial strain 2002; F ₃: bacterial strain QZ-2000+ bacterial strain 2003; F ₄: bacterial strain QZ-2000+ bacterial strain 2001+ bacterial strain 2002; F ₅: bacterial strain QZ-2000+ bacterial strain 2001+ bacterial strain 2003; F ₆: bacterial strain QZ-2000+ bacterial strain 2002+ bacterial strain 2003; F ₇: bacterial strain QZ-2000+ bacterial strain 2001+ bacterial strain 2003+ bacterial strain 2004 sprays application to respectively on the lady's-grass of 1.5-4.5 leaf age phase then.Preserved moisture 32 ℃ of average temperature, each 5 repetition 12 hours.Handle after three days, bacterial strain 2001, bacterial strain 2002,2003 pairs of mould genus bacterial strains of hyperbolic spore of bacterial strain QZ-2000 have significant synergism, make the pathogenicity rate of lady's-grass increase to 92.3%, 85.6% and 87.7% by 83.7%; Bacterial strain QZ-2000+ bacterial strain 2001+ bacterial strain 2002, bacterial strain QZ-2000+ bacterial strain 2001+ bacterial strain 2002, bacterial strain QZ-2000+ bacterial strain 2002+ bacterial strain 2003 make the pathogenicity rate of lady's-grass increase to 95.7%, 96.3%, 89.7%; The spore of four kinds of fungies add together the back synergy bigger, pathogenicity rate increases to 98.3%.Embodiment 4: the method for pressing embodiment 2, produce spore suspension, add some tensio-active agents or auxiliary (alginic acid is received, Yelkin TTS, tween, vegetables oil, glucose, starch, N ketone, little by 4204, N fertilizer etc.), handle weeds such as lady's-grass, barnyard grass grass, Herba Setariae Viridis, the result obviously improves the speed of killing grass, reduces by 5-12 hour moisture preserving time.

The conidium of the mould QZ-2000 of hyperbolic spore and micro-tensio-active agent or auxiliary are used, and can accelerate the onset speed that infects of this fungi, reduce the dependency to humidity.The spore suspension that is respectively the mould QZ-2000 of hyperbolic spore of 50g/L, 5g/L, 0.5g/L in concentration adds oil-Yelkin TTS-glucose emulsion (three's concentration is respectively 3 ‰, 1 ‰, 2 ‰), on sprayed application to field growing behind the mixing 14 days the lady's-grass (leaf age is in the 1.5-4.5 leaf phase), check after 15 days and the record degree of disease.Day samming is 30 ℃, each 5 repetition.

The hyperbolic spore trichoderma strain QZ-2000 of table 5 auxiliary agent and different concns uses the pathogenic concentration (g/L) to the field lady's-grass with

Dead leaf rate (%) disease index (%) 50 68.867 ± 1.73aA 80.39 ± 1.43aA 71.47 ± 2.84aA 75.73 ± 1.36aA5 63.9 ± 1.41bB 78.12+0.41aA 61.43 ± 1.54bB 69.13 ± 3.3bAB0.5 52.1 ± 2.33cC 76.77 ± 1.9aA 60.03 ± 1.43bB 62.66 ± 2.11cB auxiliary agent contrast of the sick leaf rate of fresh weight inhibiting rate (%) (%) 0dD 23.9 ± 1.75bB, 20.52 ± 1.3cC, 2.6 ± 0.2dC data in every row are big with difference; there were significant differences and utmost point significant difference (P＜0.05 and P＜0.01) embodiment 5 in the expression respectively of lowercase: lady's-grass; veronica (Veronica sp.); Siberian cocklebur (Xanthium sp.) usually mixes and is born in corn; peanut; cotton; farming field and vegetables such as soybean; the field, orchard; spore suspension and colletotrichum gloeosporioides Penz Persian veronica specialized form with the mould genus bacterial strain of hyperbolic spore QZ-2000; Alternaria zinniae is united and is used in above-mentioned farmland; the result suppresses or the lady's-grass that kills these farmlands partly constantly; veronica; weeds such as Siberian cocklebur, and barnyard grass grass and Herba Setariae Viridis had control action kou.Embodiment 6: the method for embodiment 2 is produced spore suspension, doubly acetochlor of the 1/20-1/5 that adds conventional amount used, alachlor, metolachlor, propisochlor, naftypramide, isoproturon, chlorotoluron, sencorex, oxadiazon, Diuron Tech if so, beautiful farming is happy etc., evenly spray on the lady's-grass of 1.0-2.5 leaf age phase the two groups of processing of preserving moisture respectively 12 hours and do not preserve moisture.In processing back 2 days, one group the lady's-grass Be Controlled rate of preserving moisture reached 100%, and one group the lady's-grass Be Controlled rate of not preserving moisture reaches 70-90%.Embodiment 7: after being covered with the culture dish autoclaving of filter paper, put 50 lady's-grass seeds in every ware.Spore or the mycelia suspension of the mould genus bacterial strain of hyperbolic spore QZ-2000 are imposed in the culture dish, and 2 milliliters in every ware repeats 5.Other establishes sterilized water and handles in the ware lady's-grass in contrast.After 4 days, contrast lady's-grass seed germination and growing way are all normal, and whole death are promptly infected in the lady's-grass seed that bacterium liquid is handled sprouting just.Embodiment 8: the method for embodiment 2 is produced spore suspension, that the 1/20-1/5 that adds conventional amount used taking doubly pounced on is clean, cover grass energy, high cover grass energy, quizalofopethyl, smart quizalofopethyl, quizalofop, quizalofopPethyl, surely kill, fluazifop-p-butyl, fenoxaprop-P, fenoxapropPethyl etc., evenly spray on the lady's-grass of 3-4.5 leaf age phase the two groups of processing of preserving moisture respectively 1 day and do not preserve moisture.In processing back 2 days, one group the lady's-grass Be Controlled rate of preserving moisture reached more than 99.5%, and one group the lady's-grass Be Controlled rate of not preserving moisture reaches 85-90%.Embodiment 9: the method for embodiment 2 is produced spore suspension, add conventional amount used bentazone, make that it is grand, 2 first, 4 chlorine, Dicamba, prowers, weeds burn, restrain wealthy pleasure etc., evenly spray lady's-grass in the 3-6 leaf age phase, barnyard grass grass, and multiple broadleaf weeds such as Eclipta prostrata, Herba Acalyphae, purslane etc. on, in processing back 7 days, the control effect of weeds was at 75-90%.

Claims (4)

1, a kind of fungal bacterial strain is characterized in that:

The mould fungal bacterial strain of a kind of hyperbolic spore (QZ-2000), depositary institution: China Microbial Culture Preservation Commission, preservation date: October 8 calendar year 2001, preserving number: CGMCC 0640; Imperfect fungi (FungiImperfecti), Moniliales (Moniliales), Moniliaceae (Moniliaceae), the mould genus of hyperbolic spore (NakataeaHara), the bacterium colony of QZ-2000 is olive colour or blackish green on the PDA substratum; The conidiophore list is given birth to or grown thickly, and upright, olive brown or black have barrier film, and is 48～196 * 4～6 microns, single or once in a while at the top branch, elongated; Conidium forms continuously, the shoot top that new spore life is born below previous spore, and the spore size is 30～36 * 5～21 microns, fusiformis, little curved or curved to a side, two terminal cells are little and round sharp, colourless or light color, two cells in middle part are big, and color depth produces four oily balls sometimes.Mycelia is spherical in shape.

2, the described fungal bacterial strain of claim 1 is used for the method for management of weeds, it is characterized in that:

Be that the conidium of the mould genus bacterial strain of hyperbolic spore (QZ-2000) of 3-50g/L or mycelia suspension carry out the blade face to weeds and sprays or seed treatment with concentration.

3, fungal bacterial strain according to claim 2 is used for the method for management of weeds, it is characterized in that:

The conidium of its mould genus bacterial strain of described hyperbolic spore (QZ-2000) or mycelia suspension can be used management of weeds with all kinds of auxiliarys, chemical herbicide, other lady's-grass bacterial parasite, glue born of the same parents anthrax-bacilus veronica specialized form, the Alternaria zinniae bacterium of the 1/5-1/20 of conventional amount used or conventional amount used.

4, be used for the method for biological weed control according to claim 2 or 3 described fungal bacterial strains, it is characterized in that

Auxiliary refers to that alginic acid is received, tween, vegetables oil, Yelkin TTS, glucose, starch, N ketone, little by 4204, N fertilizer; Chemical herbicide refer to acetochlor, alachlor, metolachlor, propisochlor, naftypramide, isoproturon, chlorotoluron, sencorex, oxadiazon, Diuron Tech if so, Yu Nongle, take pounce on clean, cover grass energy, high cover grass energy, quizalofopethyl, smart quizalofopethyl, quizalofop, quizalofopPethyl, surely kill, fluazifop-p-butyl, fenoxaprop-P, fenoxapropPethyl, bentazone, make that it is grand, 2 first, 4 chlorine, Dicamba, prowers, weeds burn, restrain wealthy pleasure.