CN1119412C - Psorospermial anthracin strain and its application method in biological herbicide - Google Patents
Psorospermial anthracin strain and its application method in biological herbicide Download PDFInfo
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Abstract
The present invention relates to a psorospermial anthracin strain and an application method in biological herbicide, which belongs to the microbe application field for agricultural plant protection. The psorospermial anthracin strain (QZ-97a) is preserved by the China microbe strain preservation committee with the preservation number of CGMCC NO. 0483 in August 28, 2000 and belongs to the special veronica type. The conidiospore and the hypha of the special strain with the concentration of 10<5> to 10<9> per milliliter or the conidiospore of the strain and chemical herbicide with the conventional dosage of 1/8 to 1/20 are used in the biological herbicide. The psorospermial anthracin strain can be safely used in dry fields without pollution and residual toxicity, such as wheat fields, rape fields, soybean and cotton fields, corn fields, etc., and can be simultaneously used for controlling the weeds with more than 85% of target weed control effect.
Description
The present invention is a kind of colletotrichum gloeosporioides Penz bacterial strain and the method that is used for biological weed control thereof, belongs to the technical field that microorganism is applied to agriculture plant protection, prevents and kill off crop weeds, is exclusively used in preventing and kill off of farmland weed.
Along with people are residual to chemical herbicide, the understanding of environmental pollution and resistance and the development of resistance weeds, the wideling popularize of green food and Organic farming, development campelyco management of weeds has become and has produced actual active demand; Also be an emerging technology and the most active research field of weed control.It has less investment, the lead time is short, specificity is strong, environmentally safe and do not produce advantages such as pollution.The substitute products that are considered to following chemical herbicide.At present, business-like campelyco kind has Comperico and the Biochon that the DeVine that develops the beginning of the eighties and Collego and the end of the nineties release.These weedicides have played vital role on the corresponding target weeds of control, or even unique selection.Long-lasting as DeVine can guarantee to use once, and 2-3 is weeding again.Being hopeful business-like campelyco project has tens of, and wherein barnyard grass grass, wild water chestnut etc. have very tempting prospect.
Persian veronica (Veronica persica Poir) is the malignant weed in summer crops fields such as wheat class, rape, and growth of seedling such as cotton, corn, soybean are also endangered seriously, and vegetables field, orchard, lawn and garden crop Tanaka also often have generation.The distribution range of Persian veronica is extensive, and all there is the report of its generation and harm China Yangtze valley and North China, and it also is the malignant weed in Europe, West Asia and farmland, America in addition.This weeds fecundity is strong, fast growth, and vegetative period is long, and resistance is strong, and artificial, machinery and chemical control be difficulty relatively.The development campelyco is prevented and kill off the Persian veronica and not only can effectively be prevented and kill off this weeds, and environmentally safe property height.At present, still do not have the research report of the campelyco of relevant Persian veronica both at home and abroad, and the exploitation of this campelyco will there be very remarkable economical and social benefit.
The objective of the invention is at the malignant weed Persian veronica (Veronica persica Poir) in seedling stages such as summer crops fields such as wheat class, rape and cotton, corn, soybean and Tender Catchweed Bedstraw Herb etc., screen a kind of colletotrichum gloeosporioides Penz bacterial strain, be used for biological weed control, can control and prevent and treat malignant weed Persian veronica, big veronica (Veronicadidyma), wall speedwell (Veronica arvense) and Tender Catchweed Bedstraw Herb etc. safely and effectively, cost is low, pollution-free, to farm crop safety.
A kind of colletotrichum gloeosporioides Penz bacterial strain of the present invention and be used for the method for biological weed control, its content and embodiment are as follows:
Colletotrichum gloeosporioides Penz bacterial strain (QZ-97a), (depositary institution: China Microbial Culture Preservation Commission; Preservation date: on August 28th, 2000 preserving number: CGMCC NO.0483), veronica specialized form (Colletotrichumgloeosporioides Penz.f.sp veronicae Sheng Qiang et Qing Zeng), imperfect fungi (FungiImperfecti), Melanconiales (Melanconiales), colletotrichum (Colletotrichum Corda.), glue born of the same parents anthrax-bacilus (Colletotrichum gloeosporioides Penz.), the bacterium colony of bacterial strain QZ-97a is orange or pink on the PDA substratum, mycelium buries life, mycelia is separated, branch, aerial hyphae are not seen or seldom; Do not produce acervulus in the cultivation, produce a large amount of conidiums on basal plane mycelia and branch, conidiophore is short, and is unicellular, 5-20 * 2-5 μ m.Conidium is unicellular colourless, round shape or avette, and the spore size is 2-4 * 5-15 μ m, the new spore tool 1-2 that produces an oily ball, the long spore of generation time has some vacuoles that differ in size; The normal myxospore group that forms orange in the culturing process; Appressorium is circular or oval, 3-5 * 3.5-6 μ m, and acervulus is born under the stratum corneum on the morbidity host, circle or subcircular, diameter 50-240 μ m does not have bristle in the dish; Condition does not produce sclerotium in good time in substratum, diameter 20-120 μ m.
The method that above-mentioned colletotrichum gloeosporioides Penz bacterial strain is used for biological weed control is: utilizing the conidium of this specialized form bacterial strain and the concentration of mycelia is every milliliter 10
5To 10
9, perhaps be used with the conidium of this bacterium and the chemical herbicide of 1/8 to 1/20 conventional amount used.
The method that above-mentioned colletotrichum gloeosporioides Penz bacterial strain is used for biological weed control is: above-mentioned chemical herbicide instigates mainly that it is grand, Gao Teke, tribenuron-methyl, 2 first, 4 chlorine, 2,4-D, Dicamba etc.
The contriver has found the natural occurrence phenomenon of Persian, field veronica in the biological control research process to the Persian veronica, so its parasitical fungi is carried out screening study.At first separate and obtain glue born of the same parents' anthrax-bacilus veronica specialized forms (Colletotrichum gloeosporioides Penz.sp.f.veronicae Sheng Qiang et Qing Zeng) bacterial strain, discover that its specialization is strong, to crop and other most plant safety, but Persian veronica, wall speedwell, big veronica and Tender Catchweed Bedstraw Herb are had very strong infection pathogenicity rate, and the control effect after the week is more than 85%.The culture condition of microbial inoculum, production in enormous quantities method have all been carried out detailed research; The infection of this bacterium the condition of causing a disease has also been carried out particular study work.Demonstrating this bacterium has bigger potential development to become the commercialization campelyco.Can be used for rape, cotton, wheat and vegetable field and control above-mentioned 4 kinds of weeds.
At present, still do not have the report of the mycoherbicide of relevant research Persian veronica both at home and abroad, the research of this respect will provide a new good approach to solving preventing and kill off of this grass.The brown scab that the Persian veronica that the present invention collects is fallen ill and all as seen differed in size on the plant cauline leaf, scab just is a round point shape, and it is circular or irregularly shaped that the about 0.5-2.0mm of diameter, scab enlarge the back, a plurality of scab associatings cause the whole necrosis of blade when serious.Scab on the stem is a round point shape earlier, develops into ellipse or bar shaped then gradually, whole section necrosis of severe patient stem.The mycelia that the visible thickness of the sick leaf of observation does not wait under light microscopic stretches out from blade, also finds the conidium of 3 kinds of different shape features.Obtain 4 kinds of fungies after separating, its cultural characteristic and pathogenic feature are as follows:
Through observing, the bacterium colony of bacterial strain QZ-97a is orange or pink on the PDA substratum, and mycelium buries life, and mycelia is separated, branch, and aerial hyphae does not see or seldom; Do not produce acervulus in the cultivation, produce a large amount of conidiums on basal plane mycelia and branch, conidiophore is short, and is unicellular, 5-20 * 2-5 μ m.Conidium is unicellular colourless, round shape or avette, and the spore size is 2-4 * 5-15 μ m, the new spore tool 1-2 that produces an oily ball, the long spore of generation time has some vacuoles that differ in size; The normal myxospore group that forms orange in the culturing process; Appressorium is circular or oval, 3-5 * 3.5-6 μ m, and acervulus is born under the stratum corneum on the morbidity host, circle or subcircular, diameter 50-240 μ m does not have bristle in the dish; Condition does not produce sclerotium in good time in substratum, diameter 20-120 μ m.
Nature is adopted back fall ill Persian veronica cauline leaf from top to bottom illness increase the weight of gradually, the water stain shape brown of top cauline leaf tool scab, round point shape, bottom cauline leaf sheet is mashed dead to full wafer, scab Vandyke brown, visible orange in surface or peach spore layer.Do after the crosscut of disease leaf visible acervulus not bristly of microscopy and conidiums identical in a large number with culture condition.
The pathogenic symptom of spore on the veronica of Persian that anthrax-bacilus QZ-97a pure culture produces is: initial water stain shape brown scab on the cauline leaf, round point shape, quantity increases like numb spot subsequently, posterior lobe sheet or full wafer are mashed dead, then big section of stem or all rot, be Vandyke brown, the conidial layer of visible pink on it, acervulus dense arrangement.Through observing, the spore on scab surface is the same with the anthrax-bacilus spore that is sprayed.
Disease symptom under the above experiment condition is similar to natural illness.
By its characterized be: imperfect fungi (Fungi Imperfecti), Melanconiales (Melanconiales), colletotrichum (Colletotrichum Corda.), glue born of the same parents anthrax-bacilus (Colletotrichum gloeosporioides Penz.).This specialized form mainly infects Veronica (Veronica L.) plant and Tender Catchweed Bedstraw Herb (Galium aparine L.var.tenerum (Gren.et Godr.) Rehb.), to staple crop hyperimmunizations such as paddy rice, wheat, cotton, corn, soybean, rape and barleys, and for other 19 section, 32 kind of plant of trying, 26 kinds of hyperimmunizations, 6 kinds of immunity (table 1 and table 2), so can be used for crop fields such as wheat, cotton, corn, soybean, rape, Chinese cabbage and barley, management of weeds.
Its cultural method is: this bacterium can be used following culture medium culturing, and induces the generation conidium:
PDA substratum---potato dextrose agar
Potato 200g
Glucose 20g
Agar 20g
Water 1L
LEPDA substratum---Persian veronica soaks juice PDA substratum
Persian veronica cauline leaf 60g (clean and dry, pulverize, filter)
Potato 200g
Glucose 20g
Agar 20g
Distilled water 1L
The CDA substratum.---the CzapekDoxAgar substratum
NaNO3 2g
K2HPO4?1g
KCl 0.5g
MgSO4 0.5g
FeSO4 0.01g
Sucrose 30g
Agar 20g
Water 1LYEA substratum---yeast extract nutrient agar
Yeast extract 30g
Agar 20g
Water 1LPCA substratum---potato Radix Dauci Sativae nutrient agar
Potato 20g (boil 1h, filter) with dusting cover
Radix Dauci Sativae 20g (boil 1h, filter) with dusting cover
Agar 20g
Water 1LMDYEA substratum---maize glucose yeast extract nutrient agar
Semen Maydis powder 20g
Glucose 20g
Yeast extract 2g
Agar 20g
Water 1LV6A substratum---V6 vegetables juice nutrient agar
V6 vegetables juice 200ml
CaCO3 3g
Agar 20g
Water 0.8LMSSA substratum---Semen Maydis powder bean powder sucrose solids substratum
Semen Maydis powder 15g
Analysis for soybean powder 15g
Sucrose 15g
Agar 20g
Water 1L Semen Maydis powder bean powder liquid sucrose substratum is pressed Semen Maydis powder beans sucrose solids culture medium prescription, but removes agar.
The conidial production in enormous quantities of this bacterium can be carried out solid culture with any of above-mentioned solid medium.Method (seeing embodiment 2 for details) that can also liquid fermenting.Perhaps with the production of liquid-solid associating training approach, its main step comprises with Semen Maydis powder bean powder liquid sucrose substratum, colletotrichum gloeosporioides Penz was cultivated about 4 days, raffinate is cultivated in elimination, mycelia is placed on the aseptic filter paper under aseptic condition, the product spore is induced in the near-ultraviolet light irradiation (12 hours illumination/12 hour dark) of 350～380nm, and sterilized water is collected conidium.
Major advantage of the present invention and positively effect are as follows:
Colletotrichum gloeosporioides Penz veronica specialized form microbial inoculum is used for biological weed control, can be made into veronica and Tender Catchweed Bedstraw Herb specialized form microbial inoculum, can select safely between broad leaf crop and these weeds.The chemical weed control of conventional anti-broadleaf weeds almost can not be selected between broad leaf crop and these broadleaf weedss.In 42 kind of plant for examination, anthrax-bacilus QZ-97a bacterial strain insensitive (hyperimmunization) there are 29 kinds, Min Gan (immunity) has 9 kinds slightly, and responsive has a kind, and very sensitive has 3 kinds.From the form angle, the plant of plant surface accessory structure prosperity is comparatively responsive to this bacterium, and the surface has wax, silicious plant responsive inadequately to this bacterium.Grass is because blade is erect, and the blade face adsorptivity is poor, and extent of injury is less.And Veronica weeds epidermal hair and glandular hairs prosperity, blade is open and flat, and the blade face adsorptivity is strong, and degree of disease is heavier.From the classification angle, other two kinds of weeds of Veronica are also very sensitive to this bacterium, rubiaceous a kind of weeds are to this bacterium sensitivity, and each a kind of weeds of Caryophyllaceous two kinds of weeds, Mang ox seedling section, composite family, Malvaceae are responsive slightly to this bacterium, and the weeds of other most of sections are insensitive to this grass.
Table 1 is for the susceptibility of examination weeds to bacterial strain QZ-97a
The occurring degree sensitivity
The name kind name processing contrast property polygonaceae Polygonaceae of section Armstrong Polygonum aviculare L. 3.40Aa 1.40Aa-
The joyful knotweed Polygonum jucundum Meisn. 10.35Aa 8.42Aa-little lamb's-quarters Chenopium of Chenopodiaceae Chenopodiaceae serotinum L. 0.97Aa 0.89Aa-Amaranthaceae Amaranthaceae Amaranthus retroflexus Amaranthus retroflexus L. 11.64Aa 7.46Bb+
Water peanut Alternanthera philoxeroides Griseb. 0.55Aa 0.63Aa-Portulacaceae Portulaceae purslane Portulaca oleracea L. 1.14Aa 0.98Aa-Caryophyllaceae Caryophyllaceae chickweed Stellaria media (L.) Cyr. 17.9Aa 7.08Bb+
Ox chickweed Malachium aquaticum (L.) Fries 15.71Aa 8.49Ab+Cruciferae Cruceferae land cress Coronopus didymus L. 3.67Aa 2.65Aa-
Bitter cress Cardamine hirsuta L. 1.35Aa 0.78Aa-pulse family Leguminosae Herba Viviae Sativae Vicia sativa L. 5.42Aa 0Bb-
The medicago hispida Medcago hispoda Gaertn. 0.93Aa 0Aa-Oxalidaceae Oxalidaceae creeping oxalis Oxalis corniculata L. 0.15Aa 0.14Aa-Mang ox seedling Geraniaceae of section Carolina Cranesbill Herb Geranium carolinianum L. 19.13Aa 11.20Bb-Euphorbiaceae Euphorbiaceae wartwort Euphorbia helioscopia L. 1.34Aa 0.92Aa+Malvaceae Malvaceae piemarker Abutilon avicennae Gaertn. 7.07Aa 0Bb-Convolvulaceae Convolvulaceae ivy glorybind Calystegia hederacea L. 1.44Aa 0Aa-
Pharbitis nil (L.) Choisy 2.08Aa 0Aa-Labiatae Labiatae benbie Lamium amplexicaule L. 10.94Aa 0.31Bb+scrophulariaceae Scrophulariaceae veronica Veronica didyma Tenore 70.81 1.55 leads a cow +++
Wall speedwell Veronica arvensis L. 68.54 6.36 +++
Persian veronica Veronica persica Poir. 67.20 1.02 +++Rubiaceae Rubiaceae Tender Catchweed Bedstraw Herb Galium aparine L.var tenerum 34.54Aa 6.36Bb ++ composite family Compositae Horseweed Herb Conyza canadensis (L.) Cronq. 11.47Aa 0.66Bb+
Sweet wine intestines Eclipta prostrata L 1.30Aa 1.21Aa-
Field thistle Cephalanoplos segetum Kitam. 1.70Aa 1.59Aa-Gramineae Gramineae drought barnyard grass Echinochloa crusgalli (L.) Beauv.var. 0.22Aa 1.30Aa-
austro-japonensis?Ohwi
Bermuda grass Cynodon dactylon (L.) Pers. 9.00Aa 6.42Aa-
Amur foxtail Alopecurus aequalis Sobol. 8.75Aa 6.88Aa-
Wang grass Beckmannia syzigachne Steud. 2.03Aa 1.45Aa-
Annual bluegrass Poa annua L. 8.67Aa 5.54Aa-
Lady's-grass Digitaria sanguinalis (L.) Scop. 2.32Aa 2.00Aa-
Herba Eleusines Indicae Eleusine indica (l.) Gaertn. 0.11Aa 0.81Aa-
Herba Setariae Viridis Setaria viridis (L.) Beauv 0.00Aa 0.40Aa-Cyperaceae Cyperaceae small brokens nutgrass flatsedge Cyperus microiria Steud. 2.08Aa 1.67Aa-Commelianaceae Commelinaceae Herba Commelinae Commelina communis L. 2.71Aa 0.95Aa-notes: insensitive (-): pathogenicity rate is below 10%; Responsive (+) slightly: pathogenicity rate is 10～30%, and is remarkable with contrast difference: responsive (++): pathogenicity rate is 30%～60%, and is remarkable with contrast difference; Extremely responsive (+++): pathogenicity rate is more than 60%, and is remarkable with contrast difference.
Table 2 is processed 0.80AaCruciferae Brassica campestris L. CK 0Aa-pulse family soybean processing 0.63AaLeguminosae Glycine max (L.) Merr. CK 0.50Aa-Malvaceae cotton processing 0AaMalvaceae Gossypium hirsutum L. CK 0Aa-grass family wheat processing 0.54AaGramineae Triticum aestivum L CK 0.67Aa-for studying thing to the sensitiveness situation section name kind name occurring degree security Cruciferae rape of anthrax-bacilus QZ-97a
Corn is handled 0Aa
Zea?mays?L CK 0Aa -
Barley is handled 0.78Aa
Hordeum?vulgare?L. CK 0.66Aa -
Paddy rice is handled 0Aa
Oriza?sativa?L. CK 0Aa -
According to above test result, anthrax-bacilus QZ-97a is to crop and most of test plant safety, only higher to weeds susceptibility such as Veronica weeds and Tender Catchweed Bedstraw Herb, in dry lands such as wheatland, rape field, soybean, cotton field and corn field, can use safely, and can be used to control above-mentioned several weeds simultaneously.This has shown once more that anthrax-bacilus QZ-97a has and has been developed as the potentiality of preventing and kill off Persian veronica campelyco.
The present invention is used for biological weed control, be to utilize live biological own, this biology is directly to be collected in the farmland physical environment, be the natural pathogenic bacterium of the target weeds of taking place in target crop field and the environment thereof, owing to exist in the environment, so there is not in use ecological risk, in addition, this bacterial strain specialization is strong, and is safe to crop and other non-target plant, can not cause the residual harm to environment.Rapidly corruption degraded after its thalline death, its degraded product is organism capable of circulation, can be because of use does not cause polluting, thereby, environmentally safe, can be used for producing green or organic farm products.
Can reach more than 85% target weeds control effect, particularly seedling stage and old and feeble plant.Colletotrichum gloeosporioides Penz QZ-97a has the highest pathogenicity rate (table 3 and table 4) to the Persian veronica.
Table 3 bacterial strain QZ-97a and other colletotrichum gloeosporioides Penz segregative lines are to the comparison of Persian veronica virulence
Bacterial strain disease index occurring degree
QZ-97a 100Aa +++
A 42.13Bb is protected in the Shandong ++
Cucumber segregative line 22.47Cc+
Persimmon segregative line 53.64Bb ++
CK 12.56Cc o
The influence of table 4 Persian veronica strain anthrax-bacilus in age QZ-97a virulence
The vegetation period of two weeks of one week
Susceptible rate
Disease refers to that the susceptible rate % disease of % refers to %
The % cotyledon period is handled 100 AaEe, 100 100 AaEe 100
The CK 00 BbHh 02-6 leaf phases of 0 BbIj are handled 70.36 AaGg, 100 68.29 AaFf 100
0 BbIj 0 ripening stage of 00 BbHh of CK is handled 61.07 AaGh, 100 58.42 AaFf 100
CK 0.22 BbIj 20 0.24 BbHh handle 68.59 AaGg, 100 70.36 AaFf 100 20 flowering period
CK 0.29 BbIj 20 0.55 BbHh handle 88.49 AaFf, 100 100 AaEe 100 40 senescence phases
(A-B is the significance of difference between handling and contrasting to CK 11.59 BbHi 100 17.83 BbGg 100; E-J is the significance of difference that whole diseases refer to)
Embodiment: embodiment 1: from spontaneous generation in Nanjing and the Persian veronica disease plant of zhejiang and other places gather and isolate several fungies, the pathogenic of them tested.
Cauline leaf from field acquisition to ill Persian veronica, record plant disease symptom, microscopy.Carry out the separation of pathogenic fungi with Persian veronica juice nutrient agar.At the monospore of each bacterial strain of microscopically picking, be seeded in respectively on the PDA substratum, in dark, cultivate down for 25 ± 1 ℃, observe the colony characteristics of pathogenic fungi pure culture and measure each colony diameter.Each bacterium colony disk of cut-off footpath 6mm is seeded in the dull and stereotyped central authorities of PDA in addition, respectively at measuring each colony diameter behind the 7d.
Various pathogenic fungi spore suspensions with different concns spray the Persian veronica respectively, observe the pathogenic property (table 5) of each fungi.
The comparison of table 5 Persian veronica pathogenic fungi colony characteristics
Qualification result is gathered D1 D2 bacterium colony bacterial classification
Place (mm) (mm)
The sticking powdery bacterial strain QZ-101 Alternariaspp Jinhua 62.34 66.82 of 28.04 33.38+orange, bacterial strain QZ-97a anthrax-bacilus Nanjing, thickness color surface ++ dark olive powdery bacterial strain QZ-201 Fusarium Jinhua 80.82 85.45 ++ the attached coccus of the thick cotton-shaped bacterial strain QZ-301 of+white Jinhua 38.35 46.26 ++ khaki color is cotton-shaped
*D1 represents to represent the colony diameter table 6 that forms behind the 6mm bacterium colony disk 5d by the colony diameter D2 that forms behind the monospore 7d, and isolating several fungies are to the virulence of Persian veronica relatively from the veronica plant of Persian
The occurring degree bacterial classification
10
8Sp/ml 10
7Sp/ml 10
6Sp/ml 10
5Sp/ml 10
4Sp/ml bacterial strain QZ-97a 100 71.93 52.78 33.96 23.12 bacterial strain QZ-101 4.24 1.53 0.60 0.75 0.64 bacterial strain QZ-102 5.11 1.30 0.58 0.47 0.36 bacterial strain QZ-103 3.98 1.78 1.15 0.83 0.54CK 2.06 2.06 2.06 2.06 2.06 as known from Table 6, and are the highest with the pathogenicity rate of QZ-97a. Embodiment 2:the QZ-97a bacterial strain was cultivated 7 days on the PDA substratum, be inoculated in the corn soya bean liquid nutrient medium and cultivated 7 days, filter out mycelia, get spore suspension, spray with manual sprayer, be inoculated on the Persian veronica of cultivating after 30 days, preserve moisture more than 15 hours.After handling for 1 week, can kill this grass fully.Embodiment 3:the method for pressing embodiment 2, produce spore suspension, add 1/20-1/5 isoproturon, Gao Teke, tribenuron-methyl, 2 first, 4 chlorine, 2 doubly respectively, 4-D, Dicamba etc., after handling weeds such as Persian veronica, Tender Catchweed Bedstraw Herb, big veronica, can obviously improve the speed of killing grass, reduce by 5-24 hour moisture preserving time.
The conidium of colletotrichum gloeosporioides Penz QZ-97a is learned the weedicide fit applications with low the quantification, can accelerate the onset speed that infects of this fungi, reduces the dependency to humidity.In concentration is 8.75 * 10
7Its grand (40% emulsion that makes that adds positive usual amounts 1/20 dosage in the anthrax-bacilus QZ-97a spore suspension of individual spore/ml, Dow AgroSciences, positive usual amounts is that 0.32g controls green bristlegrass spirit/1L water), tribenuron-methyl (75% dry type suspension concentrate, E.I.Du Pont Company, positive usual amounts is 0.02g Flumetsulam/1L water), Gao Teke (30% suspension agent, Jiangsu Nanjing insecticide factory of pesticide research institute, positive usual amounts is 1g Gao Teke/1L water), 2 first, 4 chlorine (60% wettable powder, the chemical plant, Fushun, positive usual amounts is a 0.8g/1L water), Dicamba (48% aqua, Sandos company, 1ml/1L water).Spray application to behind the mixing on the potted plant Persian veronica, throw off freshness protection package behind the 1d that preserves moisture, 7 and 14d after check and the record degree of disease.Day samming is 18.3 ℃, each 5 repetition.
Herbicide infects the Persian Veronica to colletotrichum gloeosporioides Penz QZ-97a impact is learned in table 1, low quantification
(A-B is the significance of difference between processing and contrasting; E-J is the otherness P＜0.05 between whole disease indexs)
| Processing mode | After processing a week | After processing for two weeks | ||
| Disease index % | Disease index % | |||
| The herbicide kind | Bacterium+herbicide | Herbicide | Bacterium+herbicide | Herbicide |
| Make it grand | 100AaEe | 27.09BbHh | 100AaEe | 32.27BbH h |
| Dicamba | 93.21AaEe | 20.45BbHh | 100AaEe | 29.56BbH h |
| 2 first, 4 chlorine | 90.45AaEe | 20.89BbHh | 96.57AaEe | 22.98BbH h |
| 2，4-D | 95.31AaEe | 25.67BbHh | 100AaEe | 31.57BbH h |
| Tribenuron-methyl | 80.71AaFf | 1.18BbIi | 86.42AaFf | 2.33BbIi |
| Gao Teke | 72.43AaFGf g | 1.03AaIi | 74.26AaGg | 3.85BbIi |
| The beans grass is special | 69.16AaFG g | 5.08BbIi | 72.33AaGg | 7.57BbIi |
| Anthrax-bacilus | 68.15AaGg | 72.33AaGg | ||
| Contrast | 027BbIi | |||
Claims (3)
1. colletotrichum gloeosporioides Penz bacterial strain is characterized in that:
Colletotrichum gloeosporioides Penz bacterial strain QZ-97a, veronica specialized form (Colletotrichum gloeosporioides Penz.f.sp veronicae Sheng Qiang et Qing Zeng), imperfect fungi (Fungi Imperfecti), Melanconiales (Melanconiales), colletotrichum (Colletotrichum Corda.), glue born of the same parents anthrax-bacilus (Colletotrichumgloeosporioides Penz.), depositary institution: China Microbial Culture Preservation Commission; Preservation date: on August 28th, 2000; Preserving number: CGMCC NO.0483, the bacterium colony of bacterial strain QZ-97a is orange or pink on the PDA substratum, and mycelium buries life, and mycelia is separated, branch, and aerial hyphae does not see or seldom; Do not produce acervulus in the cultivation, produce a large amount of conidiums on basal plane mycelia and branch, conidiophore is short, and is unicellular, 5-20 * 2-5 μ m; Conidium is unicellular colourless, round shape or avette, and the spore size is 2-4 * 5-15 μ m, the new spore tool 1-2 that produces an oily ball, the long spore of generation time has some vacuoles that differ in size; The normal myxospore group that forms orange in the culturing process; Appressorium is circular or oval, 3-5 * 3.5-6 μ m, and acervulus is born under the stratum corneum on the morbidity host, circle or subcircular, diameter 50-240 μ m does not have bristle in the dish; Condition does not produce sclerotium in good time in substratum, diameter 20-120 μ m.
2. colletotrichum gloeosporioides Penz bacterial strain QZ-97a is used for the method for biological weed control, and utilizing the conidium of this specialized form bacterial strain and the concentration of mycelia is every milliliter 10
5To 10
9, perhaps be used with the conidium of this bacterium and the chemical herbicide of 1/8 to 1/20 conventional amount used.
3. colletotrichum gloeosporioides Penz bacterial strain according to claim 2 is used for the biological weed control method, it is characterized in that: above-mentioned chemical herbicide instigates mainly that it is grand, Gao Teke, tribenuron-methyl, 2 first, 4 chlorine, 2,4-D, Dicamba etc.
Priority Applications (1)
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101919412B (en) * | 2010-07-20 | 2012-07-04 | 浙江省农业科学院 | Colletotrichum Corda strain and biological herbicide containing spores thereof |
| CN102154141B (en) * | 2010-10-19 | 2012-10-03 | 中国科学院武汉病毒研究所 | Pseudomonas sp. NyZ42 strain capable of degrading tribenuron-methly and preparation method thereof |
| CN102534017B (en) * | 2012-01-20 | 2013-09-11 | 福建省农业科学院植物保护研究所 | Primer for detecting orchid colletotrichum gloeosporioides molecules and quick detection method |
| CN104480060B (en) * | 2014-12-02 | 2017-05-31 | 福建省农业科学院果树研究所 | A kind of Rapid Sporulation method of colletotrichum gloeosporioides Penz |
| CN104611428B (en) * | 2015-01-21 | 2017-01-04 | 南京农业大学 | A kind of loop-mediated isothermal amplification (LAMP) primer compositions detecting colletotrichum gloeosporioides Penz and application thereof |
| CN104542723A (en) * | 2015-02-09 | 2015-04-29 | 江苏瑞东农药有限公司 | Wettable powder compounded from penoxsulam and biological herbicide and preparation method of wettable powder |
| CN105567605A (en) * | 2016-02-19 | 2016-05-11 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. and application thereof, degumming auxiliary containing lysinibacillus sp. and preparation method of degumming auxiliary |
| CN109122727B (en) * | 2018-07-16 | 2021-02-19 | 华南农业大学 | Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide |
| CN109090144A (en) * | 2018-10-17 | 2018-12-28 | 马书文 | A kind of novel environmentally-friendly biological herbicide |
| CN110551782A (en) * | 2019-06-11 | 2019-12-10 | 华南农业大学 | Application of rhamnolipid and preparation method thereof |
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