CN101856036B - Biological weed control compound and preparation method thereof - Google Patents
Biological weed control compound and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a weed control compound and a preparation method thereof. The weed control compound is mainly composed of conidium of Curvularia fungus strain QZ-2000 with preservation number CGMCC NO.0640 and a chemical herbicide, wherein the concentration of the conidium is 1-10g/L, and the concentration of the chemical herbicide is 1/20-1/3 of a conventional dosage. The invention adopts that the conidium is compounded with the chemical herbicide; on one hand, conidium dosage is lowered, biological prevention and cure cost is lowered, and the chemical herbicide performs the synergy when compound compared with the situation that only spore is adopted; on the other hand, lowers pollution of chemical herbicide is lowered.
Description
Technical field
The present invention relates to a kind of weed control compound, specifically the built agent of a kind of spore of curved mould genus bacterial strain and chemical herbicide composition.
Background technology
Weeds are considered to one of biological epidemics that have a strong impact on Agriculture and Environment always.In the past more than one since century, chemical herbicide has been used for the control of farmland weed effectively.Yet, extensive application due to chemical herbicide, the formation of many serious problem such as resistant weeds, the decline of soil and water quality, the problems such as appearance of residual, the pollution of waterhead of weed killer herbicide and anti-(anti-) property weeds are on the rise, and a large amount of exploitations and the use of chemical herbicide, do not solve weeds harm problem fully.Along with the raising of global environmental consciousness, has the extremely favor of researcher of research of the biological weed killer of efficient activity of weeding and environmental safety.And at present, " green agriculture ", " ecological agriculture " are vigorously advocated in countries in the world, due to green agriculture in the control disease pest and weed few with or without chemical pesticide, therefore, the research and development of strengthening biological weed killer are needs of sustainable agriculture development.
Biological weed killer has similar part with chemical herbicide on product form and application technology, the main distinction is that biological weed killer is all the biological product of living mostly.Because mostly present biological weed killer is to utilize fungi, therefore the biological weed killer that will utilize the fungi development is also referred to as mycoherbicide.The application of mycoherbicide based on the epidemiology principle of plant pathology, is to make the weeds introduced disease suppress weed growth take pathogen as means, reaches the purpose of controlling weeds.Plant disease is the result of host, pathogen and standort, and therefore, the research mycoherbicide namely screens highly toxic bacterial strain, prevents and kill off the target weeds under the adapt circumstance condition.
Since biological weed killer
With
Since obtaining registration in 1981 with nineteen eighty-two respectively, successively there have been six kinds to obtain registration.Compare with the rapid expansion of this area research scale, the quantity of the biological weed killer product of new registration is also few, one of them main cause is: separate a kind of special pathogen that potential using value is arranged generally relatively easy on a certain weeds, but its later evolution is very long and complicated often, the factor of restriction biological weed killer development mainly comprises four aspects, i.e. biological factor, environmental factor, technical factor, the market factor.Biological factor comprises host's variation and the formation of resistance; Environmental factor comprises temperature and humidity, and particularly humidity is considered to affect the principal element that the biological weed killer drug effect is brought into play always; Technical factor refers to that the production of formulation and processing usually affect the development of biological weed killer, and this needs the expert of expert that weeds learn and plant pathology to make joint efforts just to complete; The main trans-corporation that the market factor refers to produce agricultural chemicals is unwilling to spend huge cost and the time is carried out development and the exploitation of biological weed killer.
Biological weed killer processing is to process by processing separating the pathogenic microorganisms that obtains with the purpose of application study, improve organism to the dependence of environmental condition (as moisture), and be easy to preservation, packing, transportation, operation and application, increase stability, biologically active, to affinity and the adhesive force of weeds, and may increase the herbicide spectrum of biological weed killer, biological weed killer incorporates in the middle of the weeds comprehensive regulation the most at last.
Knotgrass (Digitaria sp.) weeds are autumn maturing crop field malignant weeds, are distributed widely in the torrid zone, the world and Temperate Region in China, and spending to south latitude 40 degree from north latitude 50 all has its growth.At present, knotgrass has more than 300 to plant in the world, is one of 18 kinds of malignant weeds in the world.In China, lady's-grass is the malignant weed in the autumn maturing Dry crop such as corn, cotton, Chinese sorghum, soybean, peanut, sugarcane, tobacco field, is also the advantage weeds in vegetable fields, orchard and horticultural crop field, is even also the main harm weeds in southern dryland rice field.At present, prevent and kill off that lady's-grass mainly contains manually, machinery, physics and chemistry prevents and kill off several class methods.Although above the whole bag of tricks all has certain effect, due to this grass very strong fertility and resistance, manually pull out with machinery prevent and kill off all undesirable, chemical control also comparatively the difficulty.
Aspect the biological insect control research of knotgrass weeds, the inventor finds to can be used for weed control (patent No. CN01134002.9) with a kind of conidium of Curvularia fungal bacterial strain and mycelia, but also requires further study on pulling up weeds efficiently.
Summary of the invention
The objective of the invention is for the pernicious knotgrass weeds in corn, soybean, sugarcane and orchard, provide the built agent of a kind of curved mould genus bacterial strain and chemical herbicide to be used for preventing and kill off the target weeds, reduce the consumption of this bacterial strain spore.
To achieve these goals, spore and the chemical herbicide built agent of the present invention for a kind of curved mould genus bacterial strain is provided, this built agent comprises: a kind of Curvularia fungal bacterial strain QZ-2000 (depositary institution: China Microbial Culture Preservation Commission; Preservation date: October 8 calendar year 2001, preserving number: conidium CGMCC NO.0640) and the chemical herbicide that filters out, described conidium concentration is 1-10g/L, the concentration of chemical herbicide is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following carbamide herbicides: isoproturon (chemical name: 3-(4-isopropyl phenyl)-1, the 1-dimethyl urea), chlortoluron (chemical name: 1,1-dimethyl-3-(3-chloro-4-methylbenzene) urea), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following diphenyl ether herbicide: (chemical name: 2-chloro-1-(3-ethyoxyl-4-nitrophenoxy)-4-trifluoromethylbenzene), concentration is the 1/20-1/3 of conventional amount used to Oxyfluorfen.
chemical herbicide is selected from following sulfonylurea herbicide: nicosulfuron (chemical name: 2-(4, 6-dimethoxypyridin-2-carbamyl aminosulfonyl)-N, the N-dimethyl nicotinamide), flucetosulfuron (chemical name: N-((4, 6-dimethoxy-2-pyrimidine) amino)-2-(2-fluoro-1-(propionyloxy) propyl group)-3-pyridine sulfonamide), metsulfuron-methyl (chemical name: 2-((4-methoxyl group-6-methyl isophthalic acid, 3, 5-triazinyl-2-is amino) ureido sulfuryl) methyl benzoate), grand (chemical name: 1-(2-chlorphenyl sulphonyl)-3-(the 4-methyl isophthalic acid of chlorine sulphur, 3, 5-triazine-2-is amino) urea), pyrazosulfuron (chemical name: 5-((4, 6-dimethoxy-2-pyrimidine) amino) formamido group sulphonyl)-1-methylpyrazole-4-ethyl ester), bensulfuron-methyl (chemical name: 3-((4, 6-dimethoxy-2-pyrimidine) amino)-1-(2-methoxy methyl acyl group benzyl) sulfonylureas), sulfometuronmethyl (chemical name: 2-((4, 6-dimethyl-2-pyrimidine) amino carbamyl amino-sulfonyl) methyl benzoate), chlorimuronethyl (chemical name: 2-((4-chloro-6-methoxyl group-2-pyrimidine) amino carbamyl amino-sulfonyl) methyl benzoate), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following three ketones weed killer herbicide: sulphur humulone (chemical name: 2-(2-chloro-4-methylsulfonyl) benzoyl-1, hydroresorcinol), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following sulfo-amino esters of gallic acid weed killer herbicide: molinate (chemical name: N, (R)-2-(4-(5-chloro-3-fluoro-2-pyridine oxygen base) phenoxy group) propionic acid propargyl ester), benthiocarb (chemical name: N N-hexa-methylene dithiocarbamate-s-ethyl), clodinafop-propargyl (chemical name:, N-diethyl thiocarbamic acid is to the benzyl chloride ester), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following three pyridine ketone weed killer herbicides: piperazine humulone (chemical name: 4-amino-6-(1,1-dimethyl ethyl)-3-methyl mercapto-1,2,4-triazine-5-tetrahydrochysene ketone), concentration is the 1/20-1/3 of conventional amount used.
chemical herbicide is selected from following aryloxy group phenoxy propionic acid class weed killer herbicide: fenoxapropPethyl (chemical name: (R)-2-(4-(6-chloro-1, 3-benzoxazoles-2-oxygen base) phenoxy group) ethyl propionate), fluazifop (chemical name: (R)-2-(4-((5-trifluoromethyl-2-pyridine) oxygen base) phenoxy group) butyl propionate), Quizalotop-ethyl (chemical name: (R)-2-(4-(6-chlorine quinoxaline-2-oxygen base) phenoxy group) ethyl propionate), haloxyfop-r-methyl (chemical name: (R)-2-(4-(3-chloro-5-trifluoromethyl-2-pyridine oxygen base) phenoxy group) methyl propionate), cyhalofop-butyl (chemical name: (R)-2-(4 (4-cyano group-2-fluorophenoxy) phenoxy group)-butyl propionate), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following cyclic ethylene ketone weed killer herbicide: sethoxydim (chemical name: 2-(1-(ethoxy amido) butyl)-5-(2-ethylmercapto group isopropyl)-3-hydroxyl-2-cyclohexene-1-ketone), concentration is the 1/20-1/3 of conventional amount used.
2-ethyl-6-methyl-N-ethoxyl methyl-alpha-chloro acetanil), the third careless amine (chemical name: 2-chloro-N-(2 chemical herbicide is selected from following chloroacetanilide herbicides: Acetochlor (chemical name:, 6-diethyl phenyl)-N-(2-propoxyl group ethyl) acetamide), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following dinitrobenzene weed killer herbicide: Amex820 (chemical name: the N-sec-butyl-4-tert-butyl group-2, the 6-dinitroaniline), Pendimethalin (chemical name: N-(1-ethyl propyl)-2,6-dinitro-3, the 4-dimethylaniline), trefanocide (chemical name: 2,6-dinitro-N, N-diη-propyl-4-5-trifluoromethylaniline), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following triazolopyrimidine sulfonamides herbicide: penoxsuam (chemical name: 3-(2, the 2-difluoroethoxy)-N-[5,8-dimethoxy-(1,2,4) triazol (1,5-C)-2-pyrimidine radicals]-α, α, α-fluoroform phenyl-2-sulfonamide), concentration is the 1/20-1/3 of conventional amount used.
Chemical herbicide is selected from following imidazolinone herbicide: (chemical name: (R, S) 5-ethyl-2-(4-isopropyl-4-methyl-5-oxo-2-imidazolinyl) nicotinic acid), concentration is the 1/10-1/3 of conventional amount used to the pharynx of miaow grass.
Chemical herbicide is selected from following organic heterocyclic class weed killer herbicide: difenzoquat (chemical name: 1,2-dimethyl-3,5-diphenyl-azoles Methylsulfate), concentration is the 1/10-1/3 of conventional amount used.
Chemical herbicide is selected from following triazine herbicide: (chemical name: 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazines), concentration is the 1/10-1/3 of conventional amount used to atrazine.
Chemical herbicide is selected from following quinoline carboxylic acid weed killer herbicide: dichloro quinolinic acid (chemical name: 3,7-two chloro-8-Quinoline Carboxylic Acids), concentration is the 1/10-1/3 of conventional amount used.
Chemical herbicide is selected from following pyrimidinylthiobenzoate herbicides: two careless ether (chemical names: 2,6-two [(4,6-dimethoxypyridin-2-yl) oxygen] Sodium Benzoate), pyrithiobacsodium (chemical name: 2-chloro-6-(4,6-SDM-2-base sulphur) Sodium Benzoate), concentration is the 1/10-1/3 of conventional amount used.
Chemical herbicide is selected from following complex weedicide: Ding Cao An Evil humulone (chemical name: N-fourth oxygen methyl-N-(2,6-diethyl phenyl)-2-chloro-acetamide 5-the tert-butyl group-3-(2,4-two chloro-5-o-phenyl-isopropyls)-1,3,4-oxadiazoline-2-ketone), concentration is the 1/10-1/5 of conventional amount used, and described conidium concentration is 5-10g/L.
Also other auxiliary agents be should add in above-mentioned weed control compound, tween, vegetable oil, SG-40, E-1310, GY-W04, GY-NDS etc. comprised.
The present invention also provides the preparation method of this weed control compound, comprises the following steps:
(1) conidium of a kind of Curvularia fungal bacterial strain QZ-2000 is cultivated: QZ-2000 was cultivated in medium 5-8 days, contain potato, glucose and agar in this medium; Get the bacterium colony fritter and transfer in wheat bran-sawdust or wheat bran solid culture medium and to cultivate 5-9 days, bacterium is cut into small pieces to be tiled in the container of level land, and thickness 0.5-1.5cm is placed in Continuous irradiation 48-72h under black light lamp, and air-dry pulverizing gets final product to get conidial powder after sieving; Or the bacterium colony fritter is inoculated in corn flour-bean powder-liquid sucrose medium concussion cultivated 5-7 days, after elimination medium raffinate, aseptic water washing obtains mycelium, namely obtains mycelium fragment suspension after pulverizing, and lamp according to 36-48h, namely gets spore in culture dish;
(2) configuration chemical herbicide mother liquor: the chemical herbicide aqueous solution of preparation conventional amount used 1/20-1/3;
(3) spore that makes in step (1) is mixed with the chemical herbicide mother liquor of configuration in step (2), the concentration of its miospore is 1-10g/L.
Kind and the concentration of the chemical herbicide that adopts in the above-mentioned built agent product of the configuration optional use of chemical herbicide mother liquor in step (2);
Also can add other auxiliary agent when mixing in step (3), as tween, vegetable oil, SG-40, E-1310, GY-W04, GY-NDS etc.
Wherein, the QZ-2000 in step (1) and the bacterium colony fritter best incubation time in solid culture medium is 7 days, and the incubation time of bacterium colony fritter in liquid nutrient medium is 6 days; The bacterium colony fritter is after solid culture medium is cultivated, and bacterium piece tiling optimum thickness is 1cm, and the best irradiation time of black light lamp is 48h; Mycelium fragment suspension optimal lamp in culture dish is 36h according to the time.
Using method, dosage and period: use the method for spraying to implement, using dosage is that the 1/20-1/3 of spore 1-10g/L and chemical herbicide conventional amount used is composite, and the operating period is best in weed germination to the 3 leaf phase.
The present invention compared with prior art has following advantage and good effect:
1. the composite needs of spore and chemical herbicide are considered many factors: selected chemical herbicide must not hinder infecting of pathogen spore in weeding, do not affect the host to the susceptibility of pathogen and the pathogen pathogenic virulence to the host, will do the compatibility test of spore and chemical herbicide before composite; Weed killer herbicide also will be considered the safety of crop in addition; There is the method for composite use in the spore chemical herbicide, composite ratio; The complexity of the growing environment of crop etc.The chemical herbicide that the present invention is selected and the compatibility of spore are good, and under carrying out a large amount of experiments and exploring, obtained the composite using method of spore and chemical herbicide, concrete compound proportion, and utilize the spore of this curved spore mould composite from different chemical herbicide selectivity, can in different crop Tanaka safe handlings, reach good Weed control effect.
2. the present invention coordinates with chemical weed control with biological weed control, has overcome the use restriction of biological weed killer and chemical herbicide.Biological weed killer (mycoherbicide) generally needs long dew phase (humidity) and specific temperature condition to guarantee that it infects and falls ill; The selectivity of fungi is high, and host specificity is strong, and the business range of application is little; The production cost of fungal spore etc. is higher.The use of chemical herbicide has also brought many environmental problems: residual poisoning, pollution of waterhead etc., and the health that threatens people and animals.And use in the present invention spore and chemical herbicide composite, reduced on the one hand the consumption of spore, reduced use biological control cost, when having overcome to a certain extent spore and having used to the dependence of humidity, enlarged in addition the herbicide spectrum of spore, enlarged range of application, and with alone spore ratio, composite middle chemical herbicide has played synergistic effect.The composite consumption that greatly reduces chemical herbicide of spore and chemical herbicide on the other hand, reduced the pollution of chemical herbicide, make its residual quantity in environment be reduced to the scope that food security allows, crop, people and animals, environmental safety are significantly improved, can be used for producing green, even organic farm products.
Embodiment
Embodiment 1:
(1) conidium is cultivated: QZ-2000 was cultivated in medium 7 days, contain potato, glucose and agar in this medium; Get the bacterium colony fritter and transfer in wheat bran-sawdust or wheat bran solid culture medium and to cultivate 7 days, bacterium is cut into small pieces to be tiled in the container of level land, and thickness is 1cm, is placed in Continuous irradiation 48h under black light lamp, and air-dry pulverizing gets final product to get conidial powder after sieving; Or the bacterium colony fritter is inoculated in corn flour-bean powder-liquid sucrose medium concussion cultivated 6 days, after elimination medium raffinate, aseptic water washing obtains mycelium, namely obtains mycelium fragment suspension after pulverizing, and lamp according to 36h, namely gets spore in culture dish;
(2) configuration chemical herbicide mother liquor: prepare the mother liquor of 100 times of chemical herbicide nicosulfuron preparation dilutions, get 1ml nicosulfuron preparation and join in 100ml water, mix;
(3) the spore 5g that makes in step (1) is dissolved in 1000ml water with the chemical herbicide mother liquor 0.8-4ml of configuration in step (2) and mixes, the concentration of spore is 5 * 10
6Individual/ml.
Embodiment 2:
(1) conidium is cultivated: QZ-2000 was cultivated in medium 7 days, contain potato, glucose and agar in this medium; Get the bacterium colony fritter and transfer in wheat bran-sawdust or wheat bran solid culture medium and to cultivate 7 days, bacterium is cut into small pieces to be tiled in the container of level land, and thickness is 1cm, is placed in Continuous irradiation 48h under black light lamp, and air-dry pulverizing gets final product to get conidial powder after sieving; Or the bacterium colony fritter is inoculated in corn flour-bean powder-liquid sucrose medium concussion cultivated 6 days, after elimination medium raffinate, aseptic water washing obtains mycelium, namely obtains mycelium fragment suspension after pulverizing, and lamp according to 36h, namely gets spore in culture dish;
(2) configuration chemical herbicide mother liquor: the mother liquor that the dilution of preparation chemical herbicide Oxyfluorfen preparation is 100 times, get 1ml Oxyfluorfen preparation and join in 100ml water, mix;
(3) the spore 5g that makes in step (1) is dissolved in 1000ml water with step (2) in chemical herbicide mother liquor 0.6-3ml and the vegetable oil 2-10ml of configuration, SG-40 1-5g, GY-W04 0.2-1g, GY-NDS 0.2-1g mixes, and the concentration of its miospore is 5 * 10
6Individual/ml.
Embodiment 3:
(1) conidium is cultivated: QZ-2000 was cultivated in medium 7 days, contain potato, glucose and agar in this medium; Get the bacterium colony fritter and transfer in wheat bran-sawdust or wheat bran solid culture medium and to cultivate 7 days, bacterium is cut into small pieces to be tiled in the container of level land, and thickness is 1cm, is placed in Continuous irradiation 48h under black light lamp, and air-dry pulverizing gets final product to get conidial powder after sieving; Or the bacterium colony fritter is inoculated in corn flour-bean powder-liquid sucrose medium concussion cultivated 6 days, after elimination medium raffinate, aseptic water washing obtains mycelium, namely obtains mycelium fragment suspension after pulverizing, and lamp according to 36h, namely gets spore in culture dish;
(2) configuration chemical herbicide mother liquor: the mother liquor that the dilution of preparation chemical herbicide fourth grass amine Evil humulone preparation is 100 times, get 1ml Oxyfluorfen preparation and join in 100ml water, mix;
(3) the spore 5g that makes in step (1) is dissolved in 1000ml water with step (2) in chemical herbicide mother liquor 1.2-6g and the vegetable oil 2-10ml of configuration, tween 1.2-4ml mixes, the concentration of its miospore is 5 * 10
6Individual/ml.
Test example 1:
The compatibility of chemical herbicide and strain QZ-200 0 spore is tested.
Choose three kinds of concentration of every kind of chemical herbicide of 21 kinds of weed killer herbicides test: 1. conventional working concentration represents with 1.0X; 2. conventional working concentration dilution is 5 times, represents with 5.0X; 3. conventional working concentration dilution is 10 times, represents with 10X.With the germination rate of sessile drop method microscopy count detection spore, with the solution that do not add weed killer herbicide in contrast.
The germination rate aspect compares the compatibility of spore and agricultural chemicals.When conventional working concentration dilutes 10 times, all over 90%.When conventional working concentration dilutes 5 times, haloxyfop-r-methyl, Quizalotop-ethyl, atrazine, Acetochlor, isoproturon, glyphosate, chlorine sulphur are grand, the spore germination rate in isoproturon, metsulfuron-methyl, flucetosulfuron, trefanocide solution is low, all the other are all greater than 50%, and there is no each other significant difference.The result of the test of spore germination rate shows that the chemical herbicide compatibilities such as lady's-grass biocontrol bacterial strain QZ-2000 and sulphur humulone, flucetosulfuron, nicosulfuron, Pendimethalin, bensulfuron-methyl, Oxyfluorfen, Ding Evil are better, can be with its composite use.
The germination rate of table 1 variable concentrations chemical herbicide miospore
Test example 2:
Artificial planting corn and lady's-grass, spore and the chemical herbicide of strain QZ-200 0 is composite, and spore concentration is 3 * 10
5Individual/ml, in lady's-grass 2.5-3.5 leaf phase dispenser (this moment general corn reached 4 leaves more than the phase), spraying time is all in the afternoon after 15:00.Weather conditions during dispenser: daily mean temperature 20-26 ℃, per day relative moisture 60 ± 25%.
Result of the test shows, the beautiful agriculture of low dosage happy (common name: nicosulfuron) composite with spore, can reach the herbicidal effect (table 2) similar to the general chemistry weed killer herbicide.When spore is happy composite with 1/10,1/5 beautiful agriculture respectively, the preventive effect of lady's-grass is increased significantly, the fresh weight preventive effect after 4 weeks of dispenser is respectively 62.3% and 82.2%, simultaneously, processes differences for two kinds and reaches remarkable standard.This result of the test shows, under field condition, the beautiful agriculture of low dosage happy with the composite QZ-2000 microbial inoculum that can greatly improve of spore to the preventive effect of weeds.
Each processes table 22 weeks of lady's-grass, fresh weight preventive effect and strain preventive effect investigation result after 4 weeks
Test example 3:
Select different spore concentrations and chemical weed control agent concentration to process the lady's-grass seedling, in lady's-grass 3 leaf phase dispensers, not moisturizing.The preventive effect of processing after 7 days, 14 days sees Table 3.
Result of the test shows, different spore concentrations have appreciable impact to herbicidal effect, and along with spore concentration increases, herbicidal effect strengthens.When spore concentration reached 5g/L, spore and 1/10 chemical herbicide got lady's-grass strain preventive effect and reach more than 80% when composite.
Composite pathogenic to lady's-grass of the spore of table 3 variable concentrations and chemical herbicide
Test example 4:
Select the spore concentration 3g/L of Curvularia strain QZ-200 0, different chemical weed control agent concentrations is all 1/10 of conventional amount used, in lady's-grass 3 leaf phase dispensers, not moisturizing.Disease index and the fresh weight inhibiting rate processed after 14 days see Table 4.
Result of the test shows, composite effective than alone spore or alone chemical herbicide of spore and chemical herbicide, and namely both are composite plays synergistic effect.
Disease index and fresh weight inhibiting rate that table 4 spore and different chemical mixture of herbicides are prevented and kill off lady's-grass compare
Test example 5:
Select the spore concentration 1 * 10 of Curvularia strain QZ-200 0
5Individual/ml, different chemical weed control agent concentrations are 1/10 of conventional amount used, spore and mixture of herbicides.
Take 4 leaf phase lady's-grass same position (the 3rd leaf) blades of the same size, first use 75% alcohol surface sterilization, be cut into the segment that is about 5cm with aseptic water washing for several times again, every processing is got 6 leaves jog in the 10ml spore suspension and was flooded for 30 seconds, take out blade is put down (maintenance filter paper is moistening) in the culture dish that moistening filter paper is arranged, as for (12 hours dark, illumination in 12 hours) in the illumination box of 25 ℃ 2 days, take out the incidence of investigation lady's-grass excised leaf.
Pathogenic impact sees Table 4-3 to the crop field of testing chemical herbicide commonly used on lady's-grass on the conidium of activity from Curvularia eragrostidis strain QZ-2000.Pathogenic kind and the concentration thereof because of chemical herbicide of strain QZ-200 0 presents difference.Part chemical herbicide and spore are composite not to raise along with the increase of weed killer herbicide concentration to the pathogenic of lady's-grass excised leaf, may be due to weed killer herbicide, spore to be had injury, and concentration is higher, injures larger.Composite pathogenic higher to lady's-grass of nicosulfuron, Pendimethalin, bensulfuron-methyl, Oxyfluorfen, Ding Cao An Evil humulone, glyphosate and spore, and increase with weed killer herbicide concentration and improve, can tentatively be defined as the candidate chemical herbicide composite with QZ-2000.
The impact (2d) that table 5 different chemical weed killer herbicide and concentration are pathogenic on spore
Claims (8)
1. weed control compound, it is characterized in that: conidium and chemical herbicide that this built agent is mainly CGMCC NO.0640 by a kind of its preserving number of Curvularia fungal bacterial strain QZ-2000 form, described conidium concentration is 1-10g/L, and the concentration of chemical herbicide is the 1/20-1/3 of conventional amount used;
Described chemical herbicide is selected from following carbamide herbicides: isoproturon, chlortoluron; Or following diphenyl ether herbicide: Oxyfluorfen; Or following sulfonylurea herbicide: nicosulfuron, flucetosulfuron, metsulfuron-methyl, chlorine sulphur are grand, pyrazosulfuron, bensulfuron-methyl, sulfometuronmethyl, chlorimuronethyl; Or following three ketones weed killer herbicide: sulphur humulone; Or following dithiocarbamate herbicide: molinate, clodinafop-propargyl, benthiocarb; Or following three pyridine ketone weed killer herbicides: piperazine humulone; Or following aryloxy group phenoxy propionic acid class weed killer herbicide: fenoxapropPethyl, fluazifop, Quizalotop-ethyl, haloxyfop-r-methyl, cyhalofop-butyl; Or following cyclic ethylene ketone weed killer herbicide: sethoxydim; Or following chloroacetanilide herbicides: Acetochlor, the third careless amine; Or following dinitrobenzene weed killer herbicide: Amex820, Pendimethalin, trefanocide; Or following triazolopyrimidine sulfonamides herbicide: penoxsuam.
2. weed control compound according to claim 1, is characterized in that; Chemical herbicide is selected from following imidazolinone herbicide: the pharynx of miaow grass; Or following organic heterocyclic class weed killer herbicide: difenzoquat; Or following triazine herbicide: atrazine; Or following quinoline carboxylic acid weed killer herbicide: dichloro quinolinic acid; Or following pyrimidinylthiobenzoate herbicides: two careless ethers, pyrithiobacsodium; The concentration of described chemical herbicide is the 1/10-1/3 of conventional amount used.
3. weed control compound according to claim 1, it is characterized in that: chemical herbicide is selected from following complex weedicide: Ding Cao An Evil humulone, concentration is the 1/10-1/5 of conventional amount used, described conidial concentration is 5-10g/L.
4. arbitrary described weed control compound according to claim 1 to 3 is characterized in that: also comprise one or more in tween, vegetable oil, SG-40, E-1310, GY-W04, GY-NDS in described built agent.
5. the preparation method of a weed control compound is characterized in that: comprise the following steps:
(1) a kind of its preserving number of Curvularia fungal bacterial strain QZ-2000 is CGMCC NO.0640 conidium is cultivated: QZ-2000 was cultivated in medium 5-8 days, contain potato, glucose and agar in this medium; Get the bacterium colony fritter and transfer in wheat bran-sawdust or wheat bran solid culture medium and to cultivate 5-9 days, bacterium is cut into small pieces to be tiled in the container of level land, and thickness 0.5-1.5cm is placed in Continuous irradiation 48-72h under black light lamp, and air-dry pulverizing gets final product to get conidial powder after sieving; Or the bacterium colony fritter is inoculated in corn flour-bean powder-liquid sucrose medium concussion cultivated 5-7 days, after elimination medium raffinate, aseptic water washing obtains mycelium, namely obtains mycelium fragment suspension after pulverizing, and lamp according to 36-48h, namely gets spore in culture dish;
(2) configuration chemical herbicide mother liquor: the chemical herbicide aqueous solution of preparation conventional amount used 1/20-1/3;
(3) spore that makes in step (1) is mixed with the chemical herbicide mother liquor of configuration in step (2), the concentration of its miospore is 1-10g/L;
Chemical herbicide in step (2) is selected from following carbamide herbicides: isoproturon, chlortoluron; Or following diphenyl ether herbicide: Oxyfluorfen; Or following sulfonylurea herbicide: nicosulfuron, flucetosulfuron, metsulfuron-methyl, chlorine sulphur are grand, pyrazosulfuron, bensulfuron-methyl, sulfometuronmethyl, chlorimuronethyl; Or following three ketones weed killer herbicide: sulphur humulone; Or following dithiocarbamate herbicide: molinate, clodinafop-propargyl, benthiocarb; Or following three pyridine ketone weed killer herbicides: piperazine humulone; Or following aryloxy group phenoxy propionic acid class weed killer herbicide: fenoxapropPethyl, fluazifop, Quizalotop-ethyl, haloxyfop-r-methyl, cyhalofop-butyl; Or following cyclic ethylene ketone weed killer herbicide: sethoxydim; Or following chloroacetanilide herbicides: Acetochlor, the third careless amine; Or following dinitrobenzene weed killer herbicide: Amex820, Pendimethalin, trefanocide; Or following triazolopyrimidine sulfonamides herbicide: penoxsuam.
6. the preparation method of weed control compound according to claim 5, it is characterized in that: the chemical herbicide in step (2) is selected from following imidazolinone herbicide: the pharynx of miaow grass; Or following organic heterocyclic class weed killer herbicide: difenzoquat; Or following triazine herbicide: atrazine; Or following quinoline carboxylic acid weed killer herbicide: dichloro quinolinic acid; Or following pyrimidinylthiobenzoate herbicides: two careless ethers, pyrithiobacsodium; The concentration of described chemical herbicide is the 1/10-1/3 of conventional amount used.
7. the preparation method of weed control compound according to claim 5, it is characterized in that: the chemical herbicide in step (2) is selected from following complex weedicide: Ding Cao An Evil humulone, concentration is the 1/10-1/5 of conventional amount used; Conidium concentration in step (3) is 5-10g/L.
8. the preparation method of weed control compound according to claim 5 is characterized in that: when step (3) is mixed, also should add one or more in tween, vegetable oil, SG-40, E-1310, GY-W04, GY-NDS.
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| CN103121877B (en) * | 2013-02-04 | 2016-05-11 | 广东中迅农科股份有限公司 | Penoxsuam and pyrazosulfuron fertilizer granules and preparation method thereof |
| CN103404536B (en) * | 2013-08-21 | 2015-11-25 | 南京农业大学 | A kind of compound biological herbicide and using method thereof |
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| CN104522057A (en) * | 2014-12-31 | 2015-04-22 | 吴中区金庭小美华家庭农场 | Environment-friendly herbicide and preparing method of environment-friendly herbicide |
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