CN112816398A

CN112816398A - Collimator for plant microbeam irradiation experiment and automatic sample changing device

Info

Publication number: CN112816398A
Application number: CN202011610313.0A
Authority: CN
Inventors: 金文杰; 李文建; 周利斌
Original assignee: Institute of Modern Physics of CAS
Current assignee: Institute of Modern Physics of CAS
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-05-18
Anticipated expiration: 2040-12-30
Also published as: CN112816398B

Abstract

The invention discloses a collimator for a plant micro-beam irradiation experiment and an automatic sample changing device, which comprise a cylindrical main body, a collimating hole and an elastic body; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction; the cross section area of the solid part is matched with that of the beam spot, the plurality of collimation holes are uniformly arranged on the solid part, and the collimation holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimation holes one by one. The collimation hole can equally divide the round spot-shaped beam emitted by the existing irradiation equipment into a plurality of micro beams, so that a more accurate local irradiation experiment of the plant is realized, the respective functions of each part of the seeds or each part of the plant in the whole plant growth and development process can be conveniently and pertinently researched, and the mutagenesis mechanism of each position of the irradiation material can be conveniently researched.

Description

Collimator for plant microbeam irradiation experiment and automatic sample changing device

Technical Field

The invention relates to a collimator, in particular to a collimator for a plant micro-beam irradiation experiment and an automatic sample changing device.

Background

Heavy ion beam radiation is a new mutagenesis technology, has the characteristics of wide mutagenesis spectrum and strong mutagenesis capability, and has wider adaptability compared with the traditional mutagenesis technology (such as ultraviolet rays, X rays, gamma rays and the like), so that the novel mutagenesis technology is more and more important for screening mutant strains. Compared with the traditional mutagenesis technology, the heavy ion beam has the following characteristics: firstly, high LET rays generate compact ionization, so that cluster damage of DNA occurs to cell nuclei, and then DNA Double Strand Break (DSB) is generated, and the damage is not easy to repair, so that the high LET rays have high Relative Biological effect (RBE for short); the heavy ion beam can deposit a large amount of Energy on the track of the heavy ion beam when passing through a medium, and has a high Linear Energy Transfer (LET) value; a sharp energy loss Bragg peak is generated at the tail end of the ion traveling, the LET value is relatively small in a long distance and is suddenly increased to the end, so that the part of the organism which is seriously affected is local, and the influence of other parts is small; for a single biological system, physiological damage is light, damaged parts are concentrated, more mutants can be obtained, and the mutation rate is improved; and fourthly, because the heavy ion radiation induces more DSBs, the repairing effect after damage is small, a large amount of mutations can be generated, and the mutants are stable and quick.

In the existing plant heavy ion irradiation breeding experiment, the beam current output by the irradiation equipment from the irradiation port is in a spot shape, the irradiation area which can be reached by the irradiation equipment is usually all materials in a uniform light spot with the diameter of 3.5cm, although the beam spot can be properly adjusted, the adjustable degree has limitation, only partial area irradiation can be always carried out, and more accurate local and even point position irradiation research cannot be carried out, for example, a local irradiation experiment is only carried out on endosperm and embryo of seeds or tender buds of plant branches. Therefore, under the existing irradiation experiment conditions, it is urgently needed to develop an experiment device which can help the plant material to carry out the local irradiation experiment.

Disclosure of Invention

In view of the above problems, an object of the present invention is to provide a collimator for a plant micro-beam irradiation experiment, which can convert an area beam spot into a micro-beam spot, so as to perform an irradiation experiment on a local part of a plant.

In order to achieve the purpose, the invention adopts the following technical scheme: a collimator for a plant micro-beam irradiation experiment comprises a cylindrical main body, a collimating hole and an elastic body; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction; the cross section area of the solid part is matched with that of the beam spot, the plurality of collimation holes are uniformly arranged on the solid part, and the collimation holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimation holes one by one.

In some embodiments, the cylindrical body is designed as an aluminum cylinder with a diameter of 3.5cm and a height of 4 cm.

In some embodiments, the elastomer is a cowhells rubber.

In some embodiments, the collimating aperture has a diameter of 0.2 cm.

In some embodiments, the solid portion has a thickness of 1.5 cm.

In addition, the invention also provides an automatic sample changing device, which comprises the plant microbeam irradiation experiment collimator and a plurality of sample preparation trays in any embodiment, wherein each sample preparation tray is uniformly provided with a plurality of circular accommodating grooves, the cross-sectional areas of the circular accommodating grooves are matched with the cross-sectional areas of beam spots, and a plant microbeam irradiation experiment collimator is placed in each circular accommodating groove.

In some embodiments, the sample preparation tray is uniformly provided with 24 circular accommodating grooves.

In some embodiments, the diameter of the circular receiving groove is 3.5 cm.

In some embodiments, the sample preparation tray is made of PMMA material.

By adopting the technical scheme, the invention has the following advantages: the invention provides a collimator, which comprises a cylindrical main body, a collimating hole and an elastic body; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction, a plurality of collimation holes are uniformly arranged on the solid part, and the collimation holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part; the plurality of collimation holes in the solid part can equally divide the round spot-shaped beam emitted by the existing irradiation equipment into a plurality of micro beams, and the diameters of the beams are reduced, so that the positioning precision can be effectively improved, the irradiation position can be accurately positioned, the respective functions of each part of seeds or each part of plants in the whole plant growth and development process can be pertinently researched, and the mutagenesis mechanism of each position of the irradiation material can be conveniently researched.

Drawings

Fig. 1 is a front view of a collimator according to an embodiment of the present disclosure;

fig. 2 is a top view of a collimator according to an embodiment of the present disclosure;

FIG. 3 is a schematic diagram of an elastomer in a collimator according to an embodiment of the present disclosure; and

fig. 4 is a schematic view of a sample preparation tray in an automatic sample changing device according to an embodiment of the present disclosure.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "back", "inner", "outer", "transverse", "vertical", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the system or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used to define elements only for convenience in distinguishing between the elements, and unless otherwise stated have no special meaning and are not to be construed as indicating or implying any relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The embodiment of the disclosure provides a collimator for a plant micro-beam irradiation experiment and an automatic sample changing device. The collimator for the plant microbeam irradiation experiment comprises a cylindrical main body, a collimation hole and an elastic body; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction; the cross section area of the solid part is matched with that of the beam spot, a plurality of collimation holes are uniformly arranged on the solid part, and the collimation holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimation holes one by one. The collimator that this disclosed embodiment provided can use with current irradiation equipment cooperation, a plurality of collimation holes in the entity portion can equally divide the circle spot form beam current that current irradiation equipment jetted out into a plurality of little beam currents, and the seed card is established in the draw-in groove just right with the collimation hole, and then can realize the local microbeam irradiation of seed, and simultaneously, the entity portion is isolated outside the seed with all the other beam currents except for the microbeam effectively, avoid producing the interference of other factors to the experimental result, overcome and can't carry out the problem of the more accurate part of material, even the spot position irradiation experiment in plant heavy ion irradiation breeding experiment.

The collimator and the automatic sample changing device for the plant microbeam irradiation experiment provided by the embodiment of the disclosure are described in detail below with reference to the accompanying drawings.

As shown in fig. 1 to 3, a collimator for a plant microbeam irradiation experiment provided by an embodiment of the present disclosure includes a cylindrical main body 1, a collimating hole 2, and an elastic body 3; the cylindrical body 1 includes a solid portion 11, a hollow portion 12 and an open portion 13 integrally formed in a height direction; the cross-sectional area of the solid part 11 is matched with that of the beam spot, the plurality of collimation holes 2 are uniformly arranged on the solid part 11, and the collimation holes 2 penetrate through the solid part 11 and are communicated with the hollow part 12; the elastic body 3 is arranged in the hollow part 12, a plurality of clamping grooves 31 are arranged on the elastic body 3, and the clamping grooves 31 are opposite to the collimation holes 2 one by one. It will be appreciated that the volume of the slot 31 may be sized to the size of the seed so that the seed is stably retained within the slot 31.

The collimator for the plant microbeam irradiation experiment provided by the embodiment of the disclosure can be used in cooperation with the existing irradiation equipment, the plurality of collimation holes 2 on the entity part 11 can equally divide the round spot-shaped beam emitted by the existing irradiation equipment into a plurality of microbeam beams, the seed is clamped in the clamping groove 31 right opposite to the collimation holes 2, and then local microbeam irradiation of the seed can be realized, meanwhile, the entity part 11 effectively isolates the rest beams except the microbeam beams outside the seed, so that interference of other factors on the experimental result is avoided, the problem that the existing irradiation equipment cannot perform more accurate local and even point position irradiation experiment of materials in the plant heavy ion irradiation breeding experiment is overcome, and equipment support is provided for plant material research.

In some examples, the cylindrical body 1 may be designed as a cylinder with a diameter of 3.5cm and a height of 4cm, and is preferably made of aluminum.

In some examples, the elastic body 3 may be made of cowhells rubber, and the seeds may be effectively fixed by its good elasticity.

In some examples, the diameter of the collimation holes 2 is 0.2 cm.

In some examples, the thickness of the solid portion 11 is 1.5 cm.

The embodiment of the present disclosure further provides an automatic sample changing device, which includes the collimator for the plant microbeam irradiation experiment and a plurality of sample preparation trays 4 provided in any one of the above embodiments, wherein each sample preparation tray 4 is uniformly provided with a plurality of circular accommodating grooves 41, the cross-sectional area of each circular accommodating groove 41 is adapted to the beam spot area, and a collimator for the plant microbeam irradiation experiment is placed in each circular accommodating groove 41.

The automatic sample changing device provided by the embodiment of the disclosure is used in cooperation with the irradiation equipment, the automatic sample changing device can be used for placing a plurality of sample preparation trays 4 for the last time, and each sample preparation tray 4 is provided with a plurality of collimators, so that the irradiation equipment can complete local irradiation of a plurality of samples under the condition of one-time sample changing, the radiation experiment efficiency is greatly improved, and the possibility is provided for a large number of repeated experiment results.

In some examples, as shown in fig. 4, 24 circular receiving grooves 41 are uniformly formed on the sample preparation tray 4.

In some examples, the diameter of the circular receiving groove 41 is 3.5 cm.

In some examples, the sample preparation tray 4 is made of PMMA material.

The present invention has been described with reference to the above embodiments, and the structure, arrangement, and connection of the respective members may be changed. On the basis of the technical scheme of the invention, the improvement or equivalent transformation of the individual components according to the principle of the invention is not excluded from the protection scope of the invention.

Claims

1. A collimator for a plant micro-beam irradiation experiment is characterized in that: comprises a cylindrical main body, a collimation hole and an elastic body; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction; the cross section area of the solid part is matched with that of the beam spot, the plurality of collimation holes are uniformly arranged on the solid part, and the collimation holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimation holes one by one.

2. The collimator for the plant microbeam irradiation experiment as claimed in claim 1, wherein: the cylindrical main body is an aluminum cylinder with the diameter of 3.5cm and the height of 4 cm.

3. The collimator for the plant microbeam irradiation experiment as claimed in claim 1, wherein: the elastomer is made of cowhells rubber.

4. The collimator for the plant microbeam irradiation experiment as claimed in claim 1, wherein: the diameter of the collimation hole is 0.2 cm.

5. The collimator for the plant microbeam irradiation experiment as claimed in claim 1, wherein: the thickness of the solid portion is 1.5 cm.

6. The utility model provides an automatic trade appearance device which characterized in that: the collimator comprises the collimator as claimed in any one of claims 1 to 5, and a plurality of sample trays, each of which is uniformly provided with a plurality of circular receiving grooves, the cross-sectional area of the circular receiving grooves is matched with that of the beam spot, and a collimator is placed in each circular receiving groove.

7. The automated sample changing apparatus of claim 6, wherein: 24 circular holding grooves are uniformly formed in the sample preparation tray.

8. The automatic sample changing apparatus according to claim 6 or 7, wherein: the diameter of the circular accommodating groove is 3.5 cm.

9. The automated sample changing apparatus of claim 6, wherein: the sample preparation tray is made of PMMA materials.