CN112816398B

CN112816398B - Plant microbeam irradiation experiment collimator and automatic sample changing device

Info

Publication number: CN112816398B
Application number: CN202011610313.0A
Authority: CN
Inventors: 金文杰; 李文建; 周利斌
Original assignee: Institute of Modern Physics of CAS
Current assignee: Institute of Modern Physics of CAS
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2023-07-25
Anticipated expiration: 2040-12-30
Also published as: CN112816398A

Abstract

The invention discloses a plant microbeam irradiation experiment collimator and an automatic sample changing device, comprising a cylindrical main body, a collimating aperture and an elastomer; the cylindrical main body comprises a solid part, a hollow part and an opening part which are integrally formed along the height direction; the cross-sectional area of the solid part is matched with the cross-sectional area of the beam spot, a plurality of collimating holes are uniformly arranged on the solid part, and the collimating holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimating holes one by one. The collimating hole can divide the circular spot beam emitted by the existing irradiation equipment into a plurality of micro-beams uniformly, so that a more accurate local irradiation experiment of plants is realized, the respective roles of each part of seeds or each part of plants in the whole plant growth and development process can be studied in a targeted manner, and the mutagenesis mechanism of each position of an irradiation material can be studied conveniently.

Description

Plant microbeam irradiation experiment collimator and automatic sample changing device

Technical Field

The invention relates to a collimator, in particular to a collimator for plant microbeam irradiation experiments and an automatic sample changing device.

Background

Heavy ion beam radiation is used as an emerging mutagenesis technology, has the characteristics of wide mutagenesis spectrum and strong mutagenesis capability, and has wider adaptability compared with the traditional mutagenesis technology (such as ultraviolet rays, X rays, gamma rays and the like), so that the novel mutagenesis technology is more and more important for screening mutant strains. Compared with the traditional mutagenesis technology, the heavy ion beam has the following characteristics: (1) the high LET rays generate compact ionization, so that cell nuclei generate DNA cluster damage, and DNA Double Strand Break (DSB) is not easy to repair, and therefore, the damage has high relative biological effect (Relative Biological Effectiveness, RBE for short); (2) the heavy ion beam, when passing through the medium, can deposit a large amount of energy on its track, with high values of the energy transfer linear density (Linear Energy Transfer, LET); (3) a sharp energy loss Bragg peak is generated at the end of the ion travel, and at this time, the LET value is relatively small over a long path, and increases suddenly to the end, so that the part of the organism which is severely affected is local, and other parts are less affected; for a single biological system, the physiological damage is lighter, the damage parts are concentrated, more mutants can be obtained, and the mutation rate is improved; (4) because the DSB generated by heavy ion radiation induction is more, the repair effect after injury is small, a large number of mutations can be generated, and the mutants are stable and fast.

In the existing plant heavy ion irradiation breeding experiment, the beam output by the irradiation equipment from the irradiation port is in a circular spot shape, the irradiation area which can be achieved by the irradiation equipment is usually all materials in uniform light spots with the diameter of 3.5cm, although the beam spots can be moderately adjusted, the adjustable degree has limitation, only partial area irradiation can be always carried out, and more accurate local or even point irradiation research cannot be carried out, for example, local irradiation experiment can be carried out only on parts such as endosperm and embryo of seeds or tender buds of plant branches. Therefore, under the existing irradiation experiment conditions, development of an experiment device capable of helping plant materials to perform local irradiation experiments is needed.

Disclosure of Invention

In view of the above problems, one of the purposes of the present invention is to provide a collimator for plant microbeam irradiation experiments, which can convert regional beam spots into microbeam beam spots, so as to implement irradiation experiments on plant parts.

In order to achieve the above purpose, the present invention adopts the following technical scheme: a collimator for plant microbeam irradiation experiment comprises a cylindrical main body, a collimation hole and an elastomer; the cylindrical main body comprises a solid part, a hollow part and an opening part which are integrally formed along the height direction; the cross-sectional area of the solid part is matched with the cross-sectional area of the beam spot, a plurality of collimating holes are uniformly arranged on the solid part, and the collimating holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimating holes one by one.

In some embodiments, the cylindrical body is designed as an aluminum cylinder 3.5cm in diameter and 4cm in height.

In some embodiments, the elastomer is a cowhells rubber.

In some embodiments, the diameter of the collimation hole is 0.2cm.

In some embodiments, the solid portion has a thickness of 1.5cm.

In addition, the invention also provides an automatic sample changing device, which comprises the plant microbeam irradiation experiment collimator and a plurality of sample preparing trays in any embodiment, wherein each sample preparing tray is uniformly provided with a plurality of circular containing grooves, the cross section area of each circular containing groove is matched with the cross section area of a beam spot, and each circular containing groove is internally provided with a plant microbeam irradiation experiment collimator.

In some embodiments, the sample preparation tray is uniformly provided with 24 circular accommodating grooves.

In some embodiments, the circular receiving recess has a diameter of 3.5cm.

In some embodiments, the sample tray is made of PMMA material.

The invention adopts the technical proposal, and has the following advantages: the collimator provided by the invention comprises a cylindrical main body, a collimation hole and an elastomer; the cylindrical main body comprises a solid part, a hollow part and an open part which are integrally formed along the height direction, a plurality of collimating holes are uniformly arranged on the solid part, and the collimating holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part; the circular spot beam emitted by the existing irradiation equipment can be equally divided into a plurality of micro beams by the plurality of collimation holes on the solid part, and the diameter of the beams is reduced, so that the positioning precision can be effectively improved in a local irradiation experiment on plant materials, the irradiation positions can be accurately positioned, the respective functions of each part of seeds or each part of plants in the whole plant growth and development process can be purposefully studied, and meanwhile, the mutagenesis mechanism of each position of the irradiation materials can be conveniently studied.

Drawings

FIG. 1 is a front view of a collimator according to an embodiment of the present disclosure;

FIG. 2 is a top view of a collimator according to one embodiment of the present disclosure;

FIG. 3 is a schematic view of an elastomer in a collimator according to an embodiment of the disclosure; and

fig. 4 is a schematic diagram of a sample preparation tray in an automatic sample exchange device according to an embodiment of the disclosure.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. It will be apparent that the described embodiments are some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "inner", "outer", "horizontal", "vertical", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the system or element to be referred to must have a specific direction, be constructed and operated in the specific direction, and thus should not be construed as limiting the present invention. Moreover, the use of the terms first, second, etc. to define elements is merely for convenience in distinguishing the elements from each other, and the terms are not specifically meant to indicate or imply relative importance unless otherwise indicated.

In the description of the present invention, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "disposed," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

The embodiment of the disclosure provides a plant microbeam irradiation experiment collimator and an automatic sample changing device. The plant microbeam irradiation experiment collimator comprises a cylindrical main body, a collimation hole and an elastomer; the cylindrical main body comprises a solid part, a hollow part and an opening part which are integrally formed along the height direction; the cross-sectional area of the solid part is matched with the cross-sectional area of the beam spot, a plurality of collimating holes are uniformly arranged on the solid part, and the collimating holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimating holes one by one. The collimator provided by the embodiment of the disclosure can be matched with the existing irradiation equipment, a plurality of alignment holes on the entity part can divide the circular spot beam emitted by the existing irradiation equipment into a plurality of micro beam, the seeds are clamped in the clamping grooves opposite to the alignment holes, so that the local micro beam irradiation of the seeds can be realized, meanwhile, the entity part effectively isolates other beam flows except the micro beam from the seeds, the interference on other factors caused by experimental results is avoided, and the problem that more accurate local and even point irradiation experiments of materials cannot be carried out in plant heavy ion irradiation breeding experiments is solved.

The plant microbeam irradiation experiment collimator and the automatic sample changing device provided by the embodiment of the disclosure are described in detail below with reference to the accompanying drawings.

As shown in fig. 1 to 3, a collimator for plant microbeam irradiation experiment provided in an embodiment of the present disclosure includes a cylindrical main body 1, a collimation hole 2 and an elastic body 3; the cylindrical body 1 includes a solid portion 11, a hollow portion 12, and an open portion 13 integrally formed in the height direction; the cross-sectional area of the solid part 11 is matched with the cross-sectional area of the beam spot, a plurality of collimating holes 2 are uniformly arranged on the solid part 11, and the collimating holes 2 penetrate through the solid part 11 and are communicated with the hollow part 12; the elastic body 3 is arranged in the hollow part 12, a plurality of clamping grooves 31 are arranged on the elastic body 3, and the clamping grooves 31 are opposite to the alignment holes 2 one by one. It should be understood that the volume of the clamping groove 31 can be designed according to the size of the seed volume, so that the seed is stably clamped in the clamping groove 31.

The plant microbeam irradiation experiment collimator provided in the embodiment of the disclosure can be used in combination with the existing irradiation equipment, the circular spot beam emitted by the existing irradiation equipment can be equally divided into a plurality of microbeams by the plurality of collimation holes 2 on the entity part 11, and the seeds are clamped in the clamping grooves 31 opposite to the collimation holes 2, so that the local microbeam irradiation of the seeds can be realized, meanwhile, the other beam flows except the microbeams are effectively isolated from the seeds by the entity part 11, the interference on other factors generated by experimental results is avoided, the problem that in the plant heavy ion irradiation breeding experiment, the existing irradiation equipment cannot perform more accurate local or even point position irradiation experiment of the material is solved, and equipment support is provided for plant material research.

In some examples, the cylindrical body 1 may be designed as a cylinder with a diameter of 3.5cm and a height of 4cm, preferably made of aluminum.

In some examples, the elastic body 3 may be made of cowhells rubber, and the seeds may be effectively fixed by using good elasticity of elasticity.

In some examples, the diameter of the collimation holes 2 is 0.2cm.

In some examples, the thickness of the solid portion 11 is 1.5cm.

An embodiment of the present disclosure further provides an automatic sample changing device, including the plant microbeam irradiation experiment collimator and a plurality of sample preparing trays 4 provided in any one of the foregoing embodiments, each sample preparing tray 4 is uniformly provided with a plurality of circular accommodating grooves 41, a cross-sectional area of each circular accommodating groove 41 is adapted to a beam spot area, and a plant microbeam irradiation experiment collimator is placed in each circular accommodating groove 41.

The automatic sample changing device provided by the embodiment of the disclosure is matched with the irradiation equipment, a plurality of sample preparing trays 4 can be placed on the automatic sample changing device at one time, and a plurality of collimators are placed on each sample preparing tray 4, so that the irradiation equipment can finish local irradiation of a plurality of samples under one sample changing condition, the radiation experiment efficiency is greatly improved, and the possibility is provided for a large number of repeated experiment results.

In some examples, as shown in fig. 4, 24 circular receiving grooves 41 are uniformly formed in the sample preparation tray 4.

In some examples, the diameter of the circular receiving groove 41 is 3.5cm.

In some examples, the sample tray 4 is made of PMMA material.

The present invention has been described with reference to the above embodiments, and the structure, arrangement and connection of the components may be varied. On the basis of the technical scheme, the improvement or equivalent transformation of the individual components according to the principles of the invention should not be excluded from the protection scope of the invention.

Claims

1. A plant microbeam irradiation experiment collimator is characterized in that: comprises a cylindrical main body, a collimation hole and an elastomer; the cylindrical main body comprises a solid part, a hollow part and an opening part which are integrally formed along the height direction; the cross-sectional area of the solid part is matched with the cross-sectional area of the beam spot, a plurality of collimating holes are uniformly arranged on the solid part, and the collimating holes penetrate through the solid part and are communicated with the hollow part; the elastic body is arranged in the hollow part, a plurality of clamping grooves are formed in the elastic body, and the clamping grooves are opposite to the collimating holes one by one.

2. The plant microbeam irradiation experiment collimator of claim 1, wherein: the cylindrical body is an aluminum cylinder with the diameter of 3.5cm and the height of 4 cm.

3. The plant microbeam irradiation experiment collimator of claim 1, wherein: the elastic body adopts cowhells rubber.

4. The plant microbeam irradiation experiment collimator of claim 1, wherein: the diameter of the collimation hole is 0.2cm.

5. The plant microbeam irradiation experiment collimator of claim 1, wherein: the thickness of the solid part is 1.5cm.

6. An automatic sample replacing device which is characterized in that: the plant microbeam irradiation experiment collimator comprises the plant microbeam irradiation experiment collimator according to any one of claims 1 to 5 and a plurality of sample preparation trays, wherein a plurality of circular accommodating grooves are uniformly formed in each sample preparation tray, the cross-sectional area of each circular accommodating groove is matched with the cross-sectional area of a beam spot, and a plant microbeam irradiation experiment collimator is placed in each circular accommodating groove.

7. The automated sample exchange device of claim 6, wherein: 24 circular accommodating grooves are uniformly formed in the sample preparation tray.

8. The automatic sample exchange device according to claim 6 or 7, wherein: the diameter of the circular accommodating groove is 3.5cm.

9. The automated sample exchange device of claim 6, wherein: the sample preparation tray is made of PMMA material.