CN112798582B - Sewage detection method and application - Google Patents
Sewage detection method and application Download PDFInfo
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- CN112798582B CN112798582B CN202110080842.2A CN202110080842A CN112798582B CN 112798582 B CN112798582 B CN 112798582B CN 202110080842 A CN202110080842 A CN 202110080842A CN 112798582 B CN112798582 B CN 112798582B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
Abstract
The invention discloses a sewage detection method and application, which are used in the field of sewage detection and comprise the following steps: preparing a colorimetric device for sewage detection, wherein a cavity for loading a sample to be detected is vacuum, and a mechanism for controlling opening and closing of a channel communicated with the cavity is arranged on the cavity; a detection reagent is pre-loaded in a cavity for loading a sample to be detected in the colorimetric device, or the detection reagent is pre-loaded in a channel communicated with the cavity; opening a communication channel with the cavity, sucking the sewage sample into the cavity by the negative pressure environment in the cavity, closing the communication channel with the cavity, and sealing the sewage sample and the detection reagent in the cavity; the vibrating rod vibrates the colorimetric device, so that the sewage sample and the detection reagent are uniformly mixed, and the device is used for detecting the absorbance of the sample. The invention can realize sewage detection quickly and conveniently, avoids the pollution of the detected reagent to the environment, and can greatly improve the accuracy of sewage detection.
Description
Technical Field
The invention relates to the field of sewage detection, in particular to a sewage detection method and application.
Background
Today, the prior art in the field of mobile water analysis is represented by the so-called Photometric cuvette tests as disclosed in DE 4109118 A1. The use of these test facilities is done by manual operation. First, a water sample is taken with a pipette and sent into a test tube containing the main reagent. The tube is closed and shaken to mix the water sample and the main reagent, and then the tube is inserted into a photometer and the sample is measured. Such manual test tube testing is inconvenient and highly subject to error. The spent primary reagent is a health and environmental hazard and the tube must be handled accordingly after analysis. The method is complex in operation, high in cost and inconvenient in sewage treatment after detection, and meanwhile, bubbles are easily generated in the sewage detection reaction process, so that the result of sewage detection is influenced to a certain extent, and certain defects exist.
Disclosure of Invention
The invention aims to provide a sewage detection method and application, which can quickly and conveniently realize sewage detection, avoid the pollution of a detected reagent to the environment and improve the accuracy of sewage detection.
The invention is realized by the following steps:
a sewage detection method comprises the following steps:
s100, preparing a colorimetric device for sewage detection, wherein a cavity for loading a sample to be detected is vacuum, and a mechanism for controlling opening and closing of a channel communicated with the cavity is arranged on the cavity;
s200, pre-loading a detection reagent for sewage detection in a cavity for loading a sample to be detected in a colorimetric device, or pre-loading a detection reagent for sewage detection in a channel communicated with the cavity;
s300, opening a communication channel with the cavity, sucking a sewage sample to be detected into the cavity by a negative pressure environment in the cavity, closing the communication channel with the cavity after sampling and feeding of sewage are completed, and sealing the sewage sample and a detection reagent in the cavity;
s400, an external vibrating rod is used for vibrating the colorimetric device, so that the sewage sample and the detection reagent are uniformly mixed, and after the sewage sample and the detection reagent are fully reacted, the device is placed into a measuring instrument for detecting the absorbance of the sample.
In step S400, before the apparatus is sent to the detection instrument, the sewage sample in the chamber is transferred to the bubbles generated during the reaction between the sewage sample and the detection reagent.
The colorimetric device comprises a body and a protective shell coated outside the body, wherein the body is provided with a cylindrical valve cavity and a sample cavity, 1-2 feeding channels communicated with the valve cavity and the sample cavity are arranged on the body, a valve rod used for controlling the conduction of the feeding channels is movably arranged in the valve cavity, and sealing assemblies are arranged at two ends of the sample cavity.
The valve rod with the laminating of valve pocket inner wall and sliding contact, valve rod side circumference be provided with pay-off passageway assorted control channel, the valve rod top is provided with adjust knob.
The control channel adopts an annular feeding groove or a through feeding hole.
And two ends of the sample cavity are provided with sealing installation grooves, and the sealing assembly is arranged in the sealing installation grooves in a sealing mode.
The sealing assembly comprises a glass sheet installed in the sealing installation groove, a sealing gasket is arranged between the glass sheet and the bottom surface of the sealing installation groove, and a reagent pre-assembly is arranged on the inner side surface of the glass sheet.
And the middle part of the inner wall of the sample cavity is provided with an air chamber for transferring bubbles generated in the test reaction process.
The method is applied to the field of sewage detection.
(1) The invention has reasonable design, adopts a reagent preassembling method for detection, can realize the feeding of the sample to be detected only by placing the sample to be detected into the feeding air passage and rotating or vertically moving the position of the valve rod in the using process, further realizes the detection operation of the sample to be detected, greatly reduces the operation difficulty of sewage detection, and can carry out the operation without professional skills.
(2) The invention has reasonable design, adopts the capsule mode to detect the sample to be detected, and the sample treatment and test capsule can be integrally recycled after the sewage detection is finished, thereby avoiding the separate treatment of the detection test tube in the prior art, effectively reducing the pollution of the detection reagent to the environment, and simultaneously having simple structure, small volume, convenient transportation, low production cost and greatly reducing the detection cost of the sewage detection.
(3) The invention has reasonable design, and when the sewage detection is carried out, after the test air bag is horizontally arranged, the air bubbles in the sample cavity enter the air chamber, so that the air bubbles in the sample cavity can be transferred, the influence on the measurement result caused by the air bubbles left in the sample cavity is avoided, and the accuracy of the sewage detection is improved.
Drawings
FIG. 1 is a flow chart of a wastewater detection method according to the present invention;
FIG. 2 is a schematic diagram of a double feed channel structure of a colorimetric device for sewage detection according to the present invention;
FIG. 3 is a schematic diagram of a single feeding channel structure of a colorimetric device for sewage detection method and use according to the present invention.
In the figure, 1, a body; 2. a protective shell; 3. a valve cavity; 4. a sample chamber; 5. a feed channel; 6. a valve stem; 7. A seal assembly; 8. a control channel; 9. sealing the mounting groove; 10. a glass sheet; 11. a gasket; 12. a reagent package; 13. a gas chamber; 14. and adjusting a knob.
Detailed Description
The invention is further described with reference to the following figures and specific examples.
Referring to the attached fig. 1, a sewage detection method is mainly applied to the field of sewage detection, and comprises the following steps:
s100, preparing a colorimetric device for sewage detection, wherein a cavity for loading a sample to be detected is vacuum, and a mechanism for controlling opening and closing of a channel communicated with the cavity is arranged on the cavity;
s200, pre-loading a detection reagent for sewage detection in a cavity for loading a sample to be detected in a colorimetric device, or pre-loading a detection reagent for sewage detection in a channel communicated with the cavity;
s300, opening a communication channel with the cavity, sucking a sewage sample to be detected into the cavity by a negative pressure environment in the cavity, closing the communication channel with the cavity after sampling and feeding of sewage are completed, and sealing the sewage sample and a detection reagent in the cavity;
s400, using an additional vibrating rod to perform vibration operation on the colorimetric device, uniformly mixing the sewage sample and the detection reagent, and after the sewage sample and the detection reagent are fully reacted, putting the device into a measuring instrument to perform sample absorbance detection.
In step S400, before the device is sent to the detection instrument, the bubbles generated during the reaction between the sewage sample in the cavity and the detection reagent are transferred.
Referring to fig. 2 and fig. 3, the colorimetric device includes a body 1 and a protective housing 2 covering the body 1, the body 1 is provided with a cylindrical valve cavity 3 and a sample cavity 4, the body 1 is provided with 1-2 feeding channels 5 communicating the valve cavity 3 and the sample cavity 4, a valve rod 6 for controlling the conduction of the feeding channel 5 is movably arranged in the valve cavity 3, and two ends of the sample cavity 4 are provided with sealing components 7.
The valve rod 6 with the laminating of 3 inner walls in valve chamber and sliding contact, valve rod 6 side circumference be provided with 5 assorted control channel 8 of pay-off passageway, the valve rod 6 top is provided with adjust knob 14. In the embodiment, the valve rod 6 is moved up and down or horizontally rotated, so that the control channel 8 is communicated with the feeding channel 5, a sample is fed into the sample cavity 4 from the feeding channel 5 by utilizing a negative pressure environment in the sample cavity 4 and is subjected to mixing reaction with a detection reagent, and then the test capsule is placed into a measuring instrument for sample absorbance detection.
The control channel 8 adopts an annular feeding groove or a through feeding hole. In this embodiment, the annular feeding groove is a through groove provided on the circumferential surface of the valve rod 6, and the through feeding hole is a through hole provided in the valve rod 6. When the control channel 8 adopts different modes, the feeding control method of the control channel is correspondingly changed, and the method specifically comprises the following steps: when the control channel 8 adopts an annular feeding groove, the valve rod 6 is moved up and down to communicate the control channel 8 with the feeding channel 5, and then the detection medicine or the detection sample is sucked into the sample cavity 4 by utilizing negative pressure to realize the reaction between the detection sample and the detection reagent. When the control channel 8 penetrates through the feeding hole, the control channel 8 is communicated with the feeding channel 5 through rotation of the valve rod 6, and suction of detection medicines or detection samples is realized.
And sealing installation grooves 9 are formed at two ends of the sample cavity 4, and the sealing component 7 is installed in the sealing installation grooves 9 in a sealing mode. The setting of seal installation groove 9 can form the dislocation with sample chamber 4 inner wall, and then realizes the good sealing in sample chamber 4 through seal assembly 7.
The sealing assembly 7 comprises a glass sheet 10 installed in the sealing installation groove 9, a sealing gasket 11 is arranged between the glass sheet 10 and the bottom surface of the sealing installation groove 9, and a reagent pre-assembly 12 is arranged on the inner side surface of the glass sheet 10. In the present embodiment, the gasket 11 achieves good sealing between the glass sheet 10 and the bottom surface of the seal installation groove 9. The reagent package 12 is used to place a powdered or liquid assay reagent, wherein the liquid assay reagent is added to the reagent package 12 and stored frozen under vacuum.
And the middle part of the inner wall of the sample cavity 4 is provided with an air chamber 13 for transferring bubbles generated in the test reaction process. When carrying out sewage detection time measuring gasbag level horizontal back, the bubble in the sample chamber 4 enters into air chamber 13 in, can shift the bubble in the sample chamber 4, has avoided leaving the bubble in the sample chamber 4 and has caused the influence to measuring result, has promoted sewage detection's accuracy.
The specific working principle of the sample processing capsule provided by the invention is as follows: the valve rod 6 is rotated or moved up and down, the control channel 8 on the valve rod 6 is communicated with the feeding channel 5, the communicating channel of the valve cavity 3 and the sample cavity 4 is opened at the moment, the sample cavity 4 is vacuumized, the valve rod 6 is rotated or moved up and down, the channel is closed, the sample cavity 4 is sealed, and the reagent pre-assembly 12 in the sample cavity 4 is pre-filled with the detection reagent corresponding to the sample to be detected in advance.
Example 1:
when the number of the feeding channels 5 is 1, the vacuumized sample processing test capsule is taken out, the sample cavity 4 is vacuum, the position of the valve rod 6 is rotated or moved up and down, the communication channel of the valve cavity 3 and the sample cavity 4 is opened, a sample to be detected is sucked into the sample cavity 4, the valve rod 6 is pushed or rotated, the channel is closed, the sample processing test capsule vibrates under the action of an additional vibration rod, so that the medicine and the sample are uniformly mixed, finally, the test capsule which fully reacts is horizontally arranged, and the bubbles in the test capsule are horizontally arranged in a measuring instrument after entering the air chamber 13 for measuring the absorbance of the sample.
Example 2:
when the number of the feeding channels 5 is 2, taking out the sample processing test capsules, firstly pushing or rotating the valve rod 6 to enable the annular feeding groove or the through feeding hole on the valve rod 6 to be positioned at one feeding channel 5 arranged on the side wall of the valve cavity 3, opening a communication channel between the valve cavity 3 and the sample cavity 4, vacuumizing the sample cavity 4, then pushing or rotating the valve rod 6, and closing the channel; and then the valve rod 6 is pushed, the other feeding channel 5 is opened, so that a communication channel between the valve cavity 4 and the sample cavity 3 is opened, a sample to be detected is automatically sucked into the sample cavity 4 through the feeding channel 5, the valve rod 6 is pushed or the valve rod 6 is rotated, the channel is closed, the sample processing and testing capsule vibrates under the action of an additional vibrating rod, so that medicines and the sample are uniformly mixed, finally the sample processing and testing capsule is horizontally arranged, the air chamber 13 is upwards, so that bubbles generated in the sample cavity 4 enter the air chamber 13, and after the bubble transfer is completed, the testing air chamber is horizontally arranged in a measuring instrument for detecting the absorbance of the sample.
The present invention is not limited to the above embodiments, and any modifications, equivalent replacements, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. A sewage detection method is characterized by comprising the following steps:
s100, preparing a colorimetric device for sewage detection, wherein a cavity for loading a sample to be detected is vacuum, and a mechanism for controlling opening and closing of a channel communicated with the cavity is arranged on the cavity;
s200, pre-loading a detection reagent for sewage detection in a cavity for loading a sample to be detected in a colorimetric device, or pre-loading the detection reagent for sewage detection in a channel communicated with the cavity;
s300, opening a communication channel with the cavity, sucking a sewage sample to be detected into the cavity by a negative pressure environment in the cavity, closing the communication channel with the cavity after sampling and feeding of sewage are completed, and sealing the sewage sample and a detection reagent in the cavity;
s400, using an additional vibrating rod to vibrate the colorimetric device, uniformly mixing the sewage sample and the detection reagent, and after the sewage sample and the detection reagent are fully reacted, putting the device into a measuring instrument to detect the absorbance of the sample;
in step S400, before the device is sent to a detection instrument, bubbles generated in the reaction process of the sewage sample in the cavity and the detection reagent are transferred; the colorimetric device comprises a body (1) and a protective shell (2) coated outside the body (1), wherein the body (1) is provided with a columnar valve cavity (3) and a sample cavity (4), 1-2 feeding channels (5) communicated with the valve cavity (3) and the sample cavity (4) are arranged on the body (1), a valve rod (6) used for controlling the conduction of the feeding channels (5) is movably arranged in the valve cavity (3), and sealing components (7) are arranged at two ends of the sample cavity (4); the valve rod (6) is attached to the inner wall of the valve cavity (3) and in sliding contact with the inner wall, a control channel (8) matched with the feeding channel (5) is arranged on the side circumferential surface of the valve rod (6), and an adjusting knob (14) is arranged at the top end of the valve rod (6); the control channel (8) adopts an annular feeding groove or a through feeding hole; two ends of the sample cavity (4) are provided with sealing installation grooves (9), and the sealing component (7) is installed in the sealing installation grooves (9) in a sealing mode; the sealing assembly (7) comprises a glass sheet (10) arranged in the sealing installation groove (9), a sealing gasket (11) is arranged between the glass sheet (10) and the bottom surface of the sealing installation groove (9), and a reagent pre-assembly (12) is arranged on the inner side surface of the glass sheet (10); the middle part of the inner wall of the sample cavity (4) is provided with an air chamber (13) for transferring bubbles generated in the test reaction process; the method is applied to the field of sewage detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110080842.2A CN112798582B (en) | 2021-01-21 | 2021-01-21 | Sewage detection method and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110080842.2A CN112798582B (en) | 2021-01-21 | 2021-01-21 | Sewage detection method and application |
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| CN112798582A CN112798582A (en) | 2021-05-14 |
| CN112798582B true CN112798582B (en) | 2023-03-21 |
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Citations (6)
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| CN202372481U (en) * | 2011-12-21 | 2012-08-08 | 长春工程学院 | Opposite-phase high performance liquid chromatography detection device for total nitrogen in sewage |
| CN104374699A (en) * | 2014-11-12 | 2015-02-25 | 宁波美康盛德生物科技有限公司 | Multi-reagent colorimeter tube for portable colorimetric device |
| CN104568689A (en) * | 2013-10-29 | 2015-04-29 | 中国石油天然气股份有限公司 | Oilfield sewage suspended matter content monitoring device and oilfield sewage suspended matter content monitoring method |
| CN104568782A (en) * | 2013-10-15 | 2015-04-29 | 楚广诣 | Water quality analysis instrument |
| CN205898669U (en) * | 2016-08-03 | 2017-01-18 | 深圳市七善科技有限公司 | Integral type water quality analyzer |
| CN207488180U (en) * | 2017-11-29 | 2018-06-12 | 赣州华兴钨制品有限公司 | A kind of spectrophotometer automatic sampling apparatus |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109541244A (en) * | 2018-10-30 | 2019-03-29 | 长沙塞克陆德医疗科技有限公司 | A kind of iodine element automatic detector and its control method |
| CN110632055B (en) * | 2019-11-21 | 2020-03-03 | 山东畜牧兽医职业学院 | Poultry house waterline water quality sampling and detecting instrument |
| CN112666087B (en) * | 2021-01-21 | 2022-11-25 | 上海菁一科技有限公司 | Spectrophotometry test method sample treatment test capsule |
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2021
- 2021-01-21 CN CN202110080842.2A patent/CN112798582B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202372481U (en) * | 2011-12-21 | 2012-08-08 | 长春工程学院 | Opposite-phase high performance liquid chromatography detection device for total nitrogen in sewage |
| CN104568782A (en) * | 2013-10-15 | 2015-04-29 | 楚广诣 | Water quality analysis instrument |
| CN104568689A (en) * | 2013-10-29 | 2015-04-29 | 中国石油天然气股份有限公司 | Oilfield sewage suspended matter content monitoring device and oilfield sewage suspended matter content monitoring method |
| CN104374699A (en) * | 2014-11-12 | 2015-02-25 | 宁波美康盛德生物科技有限公司 | Multi-reagent colorimeter tube for portable colorimetric device |
| CN205898669U (en) * | 2016-08-03 | 2017-01-18 | 深圳市七善科技有限公司 | Integral type water quality analyzer |
| CN207488180U (en) * | 2017-11-29 | 2018-06-12 | 赣州华兴钨制品有限公司 | A kind of spectrophotometer automatic sampling apparatus |
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