CN112789503A - 分析有核红细胞的方法、血液细胞分析仪和存储介质 - Google Patents

分析有核红细胞的方法、血液细胞分析仪和存储介质 Download PDF

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CN112789503A
CN112789503A CN201880098383.7A CN201880098383A CN112789503A CN 112789503 A CN112789503 A CN 112789503A CN 201880098383 A CN201880098383 A CN 201880098383A CN 112789503 A CN112789503 A CN 112789503A
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red blood
nucleated red
blood cells
characteristic
classification
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王官振
叶波
祁欢
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2015/011—
    • G01N2015/012—
    • G01N2015/014—
    • G01N2015/016—
    • G01N2015/018—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1481—Optical analysis of particle in droplet
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles

Abstract

一种分析血液样本中的有核红细胞的方法，包括：获取血液样本中的细胞的荧光信号和散射光信号（S110）；根据荧光信号和散射光信号，对血液样本中的血影粒子、白细胞和有核红细胞进行分类和计数(S120)；获取与有核红细胞相关的特征粒子群的特征值(S130)；根据有核红细胞的分类和计数结果和特征粒子群的特征值，确定最终的有核红细胞检测结果(S140)。该方法能够极大地降低有核红细胞检测结果的假阳率和假阴率。此外，该方法还涉及一种血液细胞分析仪、用于血细胞分析仪的分析装置以及计算机可读存储介质。

Description

PCT国内申请，说明书已公开。

Claims (21)

  1. PCT国内申请，权利要求书已公开。
CN201880098383.7A 2018-12-28 2018-12-28 分析有核红细胞的方法、血液细胞分析仪和存储介质 Pending CN112789503A (zh)

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PCT/CN2018/125002 WO2020133256A1 (zh) 2018-12-28 2018-12-28 分析有核红细胞的方法、血液细胞分析仪和存储介质

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CN112789503A true CN112789503A (zh) 2021-05-11

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US (1) US11709123B2 (zh)
CN (1) CN112789503A (zh)
WO (1) WO2020133256A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114544469A (zh) * 2022-04-25 2022-05-27 深圳市帝迈生物技术有限公司 粒子分类方法及血细胞分析仪
WO2023115389A1 (zh) * 2021-12-22 2023-06-29 深圳迈瑞动物医疗科技股份有限公司 一种样本分析装置和样本分析方法

Citations (7)

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CN101086473A (zh) * 2006-06-08 2007-12-12 希森美康株式会社 试样分析用试剂、试样分析用试剂盒及试样分析方法
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CN101750476A (zh) * 2008-12-08 2010-06-23 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN101846671A (zh) * 2009-03-26 2010-09-29 希森美康株式会社 血液分析仪、血液分析方法及控制系统
CN103492875A (zh) * 2011-04-28 2014-01-01 希森美康株式会社 血液分析装置、血液分析方法及计算机程序
CN103975054A (zh) * 2011-05-04 2014-08-06 雅培制药有限公司 有核红细胞分析系统和方法
WO2016106688A1 (zh) * 2014-12-31 2016-07-07 深圳迈瑞生物医疗电子股份有限公司 一种有核红细胞报警方法、装置及流式细胞分析仪

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JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
JP4817450B2 (ja) * 2007-01-31 2011-11-16 シスメックス株式会社 血液分析装置、血液分析方法およびコンピュータプログラム
JP6710512B2 (ja) * 2015-09-14 2020-06-17 シスメックス株式会社 血液分析装置、血液分析方法及びコンピュータプログラム

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101086473A (zh) * 2006-06-08 2007-12-12 希森美康株式会社 试样分析用试剂、试样分析用试剂盒及试样分析方法
CN101097181A (zh) * 2006-06-26 2008-01-02 希森美康株式会社 试样分析用试剂、试样分析用试剂盒及试样分析方法
CN101750476A (zh) * 2008-12-08 2010-06-23 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN101846671A (zh) * 2009-03-26 2010-09-29 希森美康株式会社 血液分析仪、血液分析方法及控制系统
CN103492875A (zh) * 2011-04-28 2014-01-01 希森美康株式会社 血液分析装置、血液分析方法及计算机程序
CN103975054A (zh) * 2011-05-04 2014-08-06 雅培制药有限公司 有核红细胞分析系统和方法
WO2016106688A1 (zh) * 2014-12-31 2016-07-07 深圳迈瑞生物医疗电子股份有限公司 一种有核红细胞报警方法、装置及流式细胞分析仪

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023115389A1 (zh) * 2021-12-22 2023-06-29 深圳迈瑞动物医疗科技股份有限公司 一种样本分析装置和样本分析方法
CN114544469A (zh) * 2022-04-25 2022-05-27 深圳市帝迈生物技术有限公司 粒子分类方法及血细胞分析仪
CN114544469B (zh) * 2022-04-25 2022-10-21 深圳市帝迈生物技术有限公司 粒子分类方法及血细胞分析仪

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