CN112782388A - Growth core test device for researching ecological function of hypha outside roots of indigenous AMF - Google Patents
Growth core test device for researching ecological function of hypha outside roots of indigenous AMF Download PDFInfo
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- CN112782388A CN112782388A CN202110090513.6A CN202110090513A CN112782388A CN 112782388 A CN112782388 A CN 112782388A CN 202110090513 A CN202110090513 A CN 202110090513A CN 112782388 A CN112782388 A CN 112782388A
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- 230000012010 growth Effects 0.000 title claims abstract description 70
- 238000012360 testing method Methods 0.000 title claims abstract description 14
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- 238000005192 partition Methods 0.000 claims description 10
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- 239000012528 membrane Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 18
- 240000008042 Zea mays Species 0.000 description 17
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 16
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 14
- 235000005822 corn Nutrition 0.000 description 14
- 229910001385 heavy metal Inorganic materials 0.000 description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 11
- 239000011574 phosphorus Substances 0.000 description 11
- 229910052698 phosphorus Inorganic materials 0.000 description 11
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- 229910052725 zinc Inorganic materials 0.000 description 2
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- G01N33/245—
Abstract
The invention discloses a growth core test device for researching the ecological function of hyphae outside roots of indigenous AMF (artificial mechanical membrane), which comprises a hollow planting box with an opening at the upper end, wherein a plurality of planting positions are arranged at the bottom of the planting box, a clamping hole is formed downwards in each planting position, each planting position can be detachably connected with a growth core with a hollow opening at the upper end, a clamping block is arranged at the bottom of each growth core, a breaking pipe with openings at two ends is sleeved outside each growth core, nylon nets are arranged on the inner wall and the outer wall of each growth core, a rotating ring is arranged at the upper end of each breaking pipe, an annular connecting groove which is rotatably connected with the corresponding growth core is formed upwards in the lower end of each rotating ring, a plurality of first through holes are formed in the side wall of each growth core, and a plurality of second through holes are formed in the side wall of each breaking pipe. The invention breaks out the hyphae outside the native AMF roots by rotating the rotating ring, compares the differences of the physicochemical property of the soil between the rotating and static treatments, the absorption and growth of plant elements and the like, and indirectly reflects the ecological function of the hyphae outside the native AMF roots.
Description
Technical Field
The invention relates to the technical field of experimental study on heavy metal pollution and arbuscular mycorrhizal fungi in farmland, in particular to a growth core test device for studying the ecological function of indigenous AMF (Amf) ectorhizosphere hyphae.
Background
Yunnan is one of the important lead and zinc mining areas in China, the production and smelting of the lead and zinc mining areas cause heavy metal pollution of different degrees to peripheral farmland soil, the farmland soil is an important foundation for human survival, and the improvement of the farmland soil property and the improvement of the crop quality and yield are important tasks in recent years. Arbuscular Mycorrhizal Fungi (AMF) are a type of indigenous microorganisms that are ubiquitous in farmland soil, can form a reciprocal symbiotic system with most of terrestrial higher plants, and have attracted extensive attention for their ecological functions. Researches show that in the heavy metal contaminated soil, the AMF promotes the absorption and utilization of mineral nutrients (especially phosphorus) and water in the soil by plants, influences the formation and the stability of soil aggregates, and improves the tolerance of the plants in the heavy metal contaminated soil.
In the past, the soil is mainly sterilized, original indigenous microorganisms in the soil need to be killed, then a single artificial AMF microbial inoculum is inoculated, the plant root system and AMF hyphae grow together, and the influence of the AMF hyphae on the plant growth is analyzed through mathematical analysis (such as path analysis) or a conceptual model, but the influence of the AMF hyphae and the plant root system on the soil physicochemical property cannot be accurately distinguished. In recent years, students also adopt a compartment net-separating culture device to separate plant roots from AMF (American society for plant) external hyphae, and inoculate an artificial AMF microbial inoculum after soil sterilization to study the ecological function of the specific AMF external hyphae, but the environmental conditions of sterilized soil and undisturbed soil are greatly different, so that the effect of soil indigenous microorganisms is eliminated, and the ecological function of the farmland soil indigenous AMF external hyphae cannot be accurately reflected.
Disclosure of Invention
The invention aims to provide a growth core test device for researching the ecological function of the hyphae outside the roots of the indigenous AMF, which adopts non-sterile soil and reserves all indigenous microorganisms in the soil. The method realizes uninterrupted or interrupted treatment of hyphae outside the roots of the native AMF by a non-rotating (static) or rotating ring, contrastively analyzes index changes such as soil physicochemical property, plant growth, element absorption and the like between interrupted and uninterrupted treatment of hyphae outside the roots of the native AMF, and indirectly reflects the ecological functions of the hyphae outside the roots of the native AMF in the aspects of influencing the soil physicochemical property, the plant growth, the element absorption and the like.
The technical purpose of the invention is realized by the following technical scheme:
a growth core test device for researching the ecological function of hyphae outside roots of native AMF comprises a hollow planting box with an opening at the upper end, wherein clamping strips are arranged on two opposite inner walls of the planting box along the length direction, a plurality of partition plates are connected in the planting box in a sliding mode along the length direction, and sliding grooves in sliding connection with the corresponding clamping strips are formed in two sides of each partition plate; the bottom of planting the case is provided with a plurality of planting positions, and each planting position has all seted up the card hole downwards, and each planting position all can be dismantled and is connected with cavity upper end open-ended growth core, the bottom of every growth core all be provided with card hole matched with fixture block, every growth core all overcoat have both ends opening and rotate the interrupt pipe of being connected with it, and the inside and outside wall of every growth core all is provided with the detachable nylon wire, and the upper end of every break pipe all is provided with the swivel becket, and the lower extreme of each swivel becket all upwards sets up and corresponds the cyclic annular spread groove that growth core rotated and is connected, and a plurality of first through-holes have all been seted up to the lateral wall of every growth core, the lateral wall of every break pipe all set up a plurality ofly with the second through-hole of first through-.
By adopting the technical scheme, the following operation steps can be completed for breaking hyphae outside the native AMF roots by rotating the growth core, sampling and the like:
the method comprises the following steps: the planting box is placed at a fixed position in advance, a partition plate is slid to the middle of the planting box, the planting box is divided into two planting areas, the upper end opening of the growth core is sealed by a preservative film after the growth core, the breaking pipe and the nylon net are installed, and the growth core is installed in the planting box, so that the clamping block at the bottom of the growth core is connected with the clamping hole.
Step two: the farmland heavy metal contaminated soil is taken to be air-dried and sieved by a sieve of 80 meshes, the sterilization is not carried out, all indigenous microorganisms in the soil are reserved, then the soil is filled into a planting box, the growing core is not filled with the soil, and the natural state is recovered after the soil is placed for two weeks.
Step three: the upper port sealed by the preservative film is opened, and soil is filled into each growth core.
Step four: corn or other plants are planted in the growing core.
Step five: under the condition of a greenhouse, after the corn or other plants grow seedlings, the growth condition of the plants is observed.
Step six: according to the experimental requirements, the scales opposite to each other on the upper end face of the rotating ring 8 are taken as the standard, the breaking tube 7 of one planting area is manually rotated clockwise by 180 degrees to be overlapped with the scale, so that the hyphae outside the native AMF root are broken as a contrast, the hyphae of the AMF are statically kept growing as a treatment in the other planting area, the rotation is carried out once every three days at 6 pm, and the sampling is carried out after 10 weeks.
Step seven: the indexes of soil physicochemical property, plant growth, mineral element and heavy metal content and the like are measured, and the index change between the treatment of the hyphae outside the roots of the native AMF (American Petroleum institute) is contrastively analyzed (rotating a rotating ring) and is not interrupted (static state, namely, the rotating ring is not rotated), so that the ecological function of the hyphae outside the roots of the native AMF is indirectly reflected.
The invention is further provided with: planting the case for one side of card strip one end is provided with unloads native opening, it can dismantle and be connected with the baffle to unload native opening, unload native open-ended both sides all be provided with the cross-section and be C type and with baffle edge sliding connection's slide rail.
Through adopting above-mentioned technical scheme, pull down the baffle after the experiment of being convenient for and pour out the soil of planting the incasement.
The invention is further provided with: through holes with the same diameter are formed in the side wall of the growth core; the inner wall and the outer wall of the growth core are both provided with nylon nets.
The invention is further provided with: the aperture of the nylon net is 37 mu m.
By adopting the technical scheme, the contact area between the out-of-root hyphae of the native AMF and the soil in the planting box is increased conveniently, and the ecological function of the out-of-root hyphae of the native AMF is effectively exerted; secondly, the hypha outside the native AMF root can pass through the nylon net, but the root system can not, reduce water loss and the root system secretion can be detained in the growth core, simultaneously can accurately break the hypha outside the native AMF root when the rotating ring is rotated and the breaking tube is driven to rotate.
The invention is further provided with: the side wall of the planting box is provided with a plurality of drain holes.
The invention is further provided with: the drainage hole is 1-2 cm higher than the inner bottom of the planting box.
By adopting the technical scheme, the bottom of the planting box is kept moist, and the water can be discharged only when the water level at the bottom of the planting box reaches the height of the drain hole, so that the planting box has better water retention property.
The invention is further provided with: the surface of each rotating ring is provided with scales, the upper end of the outer side of each breaking pipe is provided with an indicating line, and the breaking pipes are manually rotated by 180 degrees, so that the hyphae outside the native AMF roots penetrating through the nylon net are broken.
Compared with the prior art, the invention has the following beneficial effects:
firstly, non-sterilized soil is adopted, on the basis of retaining all indigenous microorganisms in the soil, the scales opposite to each other on the upper end surface of the rotating ring are taken as the standard, the breaking pipe is manually rotated clockwise by 180 degrees to coincide with the scales, so that the external hyphae of the indigenous AMF roots penetrating through the nylon net are broken, and the ecological functions of the external hyphae of the indigenous AMF roots influencing the physical and chemical properties of the soil, the absorption and growth of host plant elements and the like are accurately analyzed;
secondly, a plurality of through holes are formed in the tube walls of the growth core and the breaking tube, so that the contact area between the hyphae outside the native AMF roots and the soil in the planting box can be increased, and the ecological function of the hyphae outside the native AMF roots is effectively exerted;
and thirdly, the two sides of the growth core are covered with 37-micron nylon nets, so that the water loss is reduced, root exudates can be retained in the growth core for the plant to grow, and the problem of interference of the root system and the root exudates on the research of AMF can be effectively solved.
And fourthly, the nylon net can be detached, so that the nylon net can be conveniently replaced by a new nylon net for use in the next experiment.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a view showing the connection relationship between the planting box and the partition and the baffle;
fig. 3 is used for showing the connection relationship of the growth core, the nylon net and the broken pipe.
In the figure: 1. a box body; 11. clamping the strip; 12. opening the soil unloading; 13. a slide rail; 14. a drain hole; 2. a partition plate; 21. a chute; 3. a baffle plate; 4. planting positions; 41. a clamping hole; 5. growing the core; 51. a clamping block; 52. a first through hole; 6. a nylon mesh; 7. breaking the pipe; 71. a second through hole; 8. a rotating ring; 81. and an annular connecting groove.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The embodiment of the invention, referring to fig. 1-3, a test device for studying the growth core of the hypha outside the roots of indigenous AMF comprises a hollow planting box with an opening at the upper end, two clamping strips 11 are arranged on two opposite inner walls of the planting box along the length direction, two partition plates 2 are connected in the planting box in a sliding manner along the length direction, and two sliding grooves 21 in sliding connection with the corresponding clamping strips 11 are arranged on two sides of each partition plate 2, so that the planting box can be freely divided into a plurality of planting areas according to requirements, and a comparison experiment can be conveniently carried out; one side of the planting box, which is opposite to one end of the clamping strip 11, is provided with a soil unloading opening 12, the soil unloading opening 12 is detachably connected with a baffle 3, and two sides of the soil unloading opening 12 are respectively provided with a slide rail 13 which is C-shaped in section and is in sliding connection with the edge of the baffle 3, so that the baffle 3 can be conveniently detached to pour out the soil in the planting box after the experiment is finished; the side wall of the planting box is provided with the plurality of drain holes 14, the drain holes 14 are 1 cm higher than the inner bottom of the planting box, so that the bottom of the planting box is kept moist, and the water can be drained only when the water level at the bottom of the planting box reaches the height of the drain holes 14, so that the planting box has good water-retaining property.
Nine planting positions 4 are arranged at the bottom of the planting box, each planting position 4 is downwards provided with a clamping hole 41, each planting position 4 can be detachably connected with a growth core 5 with a hollow upper end opening, the bottom of each growth core 5 is provided with a clamping block 51 matched with the clamping hole 41, each growth core 5 is externally sleeved with a breaking pipe 7 with two ends opened and rotatably connected with the growth core 5, the inner wall and the outer wall of each growth core 5 are respectively provided with a layer of detachable nylon net 6, the upper end of each breaking pipe 7 is provided with a rotating ring 8, the lower end of each rotating ring 8 is upwards provided with a circle of annular connecting groove 81 rotatably connected with the corresponding growth core 5, the side wall of each growth core 5 is provided with twelve first through holes 52, and the side wall of each breaking pipe 7 is provided with twelve second through holes 71 corresponding to the first through holes 52 one by one; the surface of each rotating ring 8 is provided with scales, the upper end of the outer side of each breaking pipe 7 is provided with an indicating line, and the breaking pipes are manually rotated by 180 degrees, so that the hyphae outside the native AMF roots passing through the nylon net 6 are broken.
In the using mode, the following operation steps can be carried out to break the hyphae outside the native AMF roots by rotating the growth core 5, sample and the like:
the method comprises the following steps: the planting box is placed at a fixed position in advance, a partition plate 2 is slid to the middle of the planting box, the planting box is divided into two planting areas, the growth core 5, the breaking pipe 7 and the nylon net 6 are installed well, the upper end opening of the growth core 5 is sealed by the preservative film and installed in the planting box, and the clamping block 51 at the bottom of the growth core 5 is connected with the clamping hole 41.
Step two: the farmland heavy metal contaminated soil is taken to be air-dried and sieved by a sieve of 80 meshes, sterilization is not needed, all indigenous microorganisms in the soil are reserved, then the soil is filled into a planting box, the growth core 5 is not filled with the soil, and the farmland heavy metal contaminated soil is placed for two weeks to recover the natural state.
Step three: the upper port sealed by the preservative film is opened, and soil is filled into each growth core 5.
Step four: corn or other plants are planted in the growing core 5.
Step five: under the condition of a greenhouse, after the corn or other plants grow seedlings, the growth condition of the plants is observed.
Step six: according to the experimental requirements, the scales opposite to each other on the upper end face of the rotating ring 8 are taken as the standard, the breaking tube 7 of one planting area is manually rotated clockwise by 180 degrees to coincide with the scale, so that the hyphae outside the roots of the native AMF are broken as a contrast, the hyphae outside the roots of the native AMF are statically kept to grow as a treatment in the other planting area, the rotation is carried out once every three days at 6 pm, and the sampling is carried out after 10 weeks.
Step seven: and measuring indexes such as soil physicochemical properties, plant growth, mineral elements, heavy metals and the like, and comparing and analyzing the index change between the interruption (rotating ring) and non-interruption (static state, non-rotating ring) treatment of the hyphae outside the roots of the native AMF to indirectly reflect the ecological function of the hyphae outside the roots of the native AMF.
Partial data analysis:
(1) influence of the indigenous AMF exo-root hyphae on the height and biomass of maize plants
As can be seen from table 1, the biomass and plant height of the upper and lower parts of the corn treated statically by the growing core device (without breaking the hyphae outside the native AMF roots) were significantly or very significantly higher than those treated by the rotation (breaking the hyphae outside the native AMF roots), indicating that the hyphae outside the native AMF roots contribute to the corn growth.
TABLE 1 plant height and Biomass of maize under static and rotating treatment of growing core apparatus
Note: static treatment: the method comprises the following steps of (1) treating the hypha growth outside the roots of the native AMF in a static state, and rotating: breaking the hypha outside the native AMF root by rotation is used as a control; *: the inter-treatment differences reached a significant level of p <0.05 x: the inter-treatment differences reached a very significant level of p <0.01, as follows.
(2) Influence of the exo-root hyphae of the indigenous AMF on the physicochemical properties of the soil
As can be seen from Table 2, the content of available phosphorus in soil statically processed by the growth core device (without breaking hyphae outside roots of the indigenous AMF) is significantly higher than that in soil rotationally processed (without breaking hyphae outside roots of the indigenous AMF), and the content of available Cd and Pb is significantly lower than that in soil rotationally processed. Therefore, the indigenous AMF rhizosphere external hyphae are beneficial to improving the content of the available phosphorus in the soil and reducing the content of the available heavy metals.
TABLE 2 content of available phosphorus and available Cd, Pb in soil by static and rotating treatment of growth core device
(3) Effect of the Ex-rooted hyphae of indigenous AMF on phosphorus absorption by maize
As can be seen from table 3, the phosphorus content in the upper part, the phosphorus absorption in the upper part and the underground part of the corn are significantly higher in the static treatment (without breaking the hyphae outside the roots of the indigenous AMF) of the growing core device than in the rotation treatment (without breaking the hyphae outside the roots of the indigenous AMF), indicating that the hyphae outside the roots of the indigenous AMF contribute to the improvement of the phosphorus nutrition of the corn plants.
TABLE 3 phosphorus content and uptake of corn under static and rotating treatment of the growth core device
(4) Influence of indigenous AMF (Amf) exohyphae on Cd and Pb contents of corn
As can be seen from Table 4, the Cd and Pb contents of the overground part and the underground part of the corn statically treated by the growth core device (without breaking the overground hyphae of the native AMF) are both significantly lower than those of the corn statically treated by the rotation treatment (without breaking the overground hyphae of the native AMF), which indicates that the overground hyphae of the native AMF contribute to reducing the heavy metal content of the corn plants.
TABLE 4 corn Cd, Pb content under static and rotating treatment of growth core device
Conclusion
In heavy metal contaminated soil, the rhizosphere external hyphae of the native AMF have ecological functions of increasing the content of available phosphorus in the soil, promoting the absorption of phosphorus by corn, improving the biomass of plants, reducing the content of heavy metal available state in the soil, reducing the absorption of heavy metal by corn and the like.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (7)
1. The utility model provides a research out-of-root hypha ecological function's of native AMF growth core test device, includes cavity upper end open-ended planting case, its characterized in that: clamping strips (11) are arranged on two opposite inner walls of the planting box along the length direction, a plurality of partition plates (2) are connected in the planting box in a sliding mode along the length direction, and sliding grooves (21) which are connected with the corresponding clamping strips (11) in a sliding mode are formed in two sides of each partition plate (2); the bottom of planting the case is provided with a plurality of planting positions (4), each planting position (4) has all seted up card hole (41) downwards, each planting position (4) all can be dismantled and be connected with cavity upper end open-ended growth core (5), the bottom of each growth core (5) all be provided with card hole (41) matched with fixture block (51), every growth core (5) all overcoat has both ends opening and rotates disconnected pipe (7) of being connected with it, the inside and outside wall of every growth core (5) all is provided with detachable nylon wire (6), the upper end of every disconnected pipe (7) all is provided with swivel becket (8), the lower extreme of each swivel becket (8) all upwards seted up with correspond growth core (5) rotate the cyclic annular spread groove (81) of being connected, a plurality of first through-holes (52) have all been seted up to the lateral wall of each growth core (5), the lateral wall of every disconnected pipe (7) all seted up a plurality of with the second through-hole (71) of first through-hole (52) one-to-one.
2. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 1, which is characterized in that: planting the case for one side of card strip (11) one end is provided with unloads native opening (12), unload native opening (12) and can dismantle and be connected with baffle (3), unload the both sides of native opening (12) all be provided with the cross-section be C type and with baffle (3) edge sliding connection's slide rail (13).
3. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 1, which is characterized in that: the growth core (5) is a hollow PVC pipe with the height of 20cm and the diameter of 7.5cm and an opening at the upper end; the side wall of the growth core (5) is provided with 12 through holes (52), and the diameter of each through hole (52) is 3 cm.
4. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 1, which is characterized in that: the aperture of the nylon net (6) is 37 mu m.
5. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 1, which is characterized in that: the side wall of the planting box is provided with a plurality of drainage holes (14).
6. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 5, wherein: the drain hole (14) is 1-2 cm higher than the inner bottom of the planting box.
7. The growth core test device for studying the ecological function of the hyphae outside the roots of the indigenous AMF according to claim 1, which is characterized in that: the surface of each rotating ring (8) is provided with scales, the upper end of the outer side of each breaking pipe (7) is provided with an indicating line, and the breaking pipes are manually rotated by 180 degrees, so that the hyphae outside the native AMF roots penetrating through the nylon net (6) are broken.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462517A (en) * | 2021-06-09 | 2021-10-01 | 东北师范大学 | Multilayer chambered device for continuously expanding propagation of arbuscular mycorrhizal fungal spores |
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2021
- 2021-01-22 CN CN202110090513.6A patent/CN112782388A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462517A (en) * | 2021-06-09 | 2021-10-01 | 东北师范大学 | Multilayer chambered device for continuously expanding propagation of arbuscular mycorrhizal fungal spores |
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