CN112775157A - Sulfonation system water-based drilling solid waste biological strengthening treatment process and greening planting soil - Google Patents
Sulfonation system water-based drilling solid waste biological strengthening treatment process and greening planting soil Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 162
- 238000005553 drilling Methods 0.000 title claims abstract description 161
- 239000002910 solid waste Substances 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 59
- 230000008569 process Effects 0.000 title claims abstract description 44
- 238000006277 sulfonation reaction Methods 0.000 title claims abstract description 43
- 239000002689 soil Substances 0.000 title claims abstract description 42
- 238000005728 strengthening Methods 0.000 title claims abstract description 20
- 239000000203 mixture Substances 0.000 claims abstract description 70
- 239000003124 biologic agent Substances 0.000 claims abstract description 41
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 21
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 14
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- 238000002156 mixing Methods 0.000 claims abstract description 14
- 241001468175 Geobacillus thermodenitrificans Species 0.000 claims abstract description 13
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 5
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- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 18
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- 238000012258 culturing Methods 0.000 claims description 16
- 239000011591 potassium Substances 0.000 claims description 16
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- 239000007789 gas Substances 0.000 claims description 9
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- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 8
- -1 NaHPO4 0.1g/L~0.3g/L Substances 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims description 6
- 239000011574 phosphorus Substances 0.000 claims description 6
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- 239000005011 phenolic resin Substances 0.000 claims description 2
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- KXGFMDJXCMQABM-UHFFFAOYSA-N 2-methoxy-6-methylphenol Chemical class [CH]OC1=CC=CC([CH])=C1O KXGFMDJXCMQABM-UHFFFAOYSA-N 0.000 description 17
- 239000003344 environmental pollutant Substances 0.000 description 17
- 231100000719 pollutant Toxicity 0.000 description 17
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- 244000005700 microbiome Species 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 7
- 230000000593 degrading effect Effects 0.000 description 6
- 239000000618 nitrogen fertilizer Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 4
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- 239000011780 sodium chloride Substances 0.000 description 4
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- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical group [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 102100021913 Sperm-associated antigen 8 Human genes 0.000 description 2
- 101710098579 Sperm-associated antigen 8 Proteins 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
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- 150000001875 compounds Chemical class 0.000 description 2
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- 238000011081 inoculation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000003209 petroleum derivative Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000029219 regulation of pH Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
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- 239000000758 substrate Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 241000131407 Brevundimonas Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000579497 Falsibacillus pallidus Species 0.000 description 1
- 241000588843 Ochrobactrum Species 0.000 description 1
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
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- 238000002386 leaching Methods 0.000 description 1
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- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
Landscapes
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides a biological strengthening treatment process for water-based drilling solid waste of a sulfonation system and greening planting soil, and the process comprises the following steps: preparing a high-temperature biological agent; the method comprises the following steps of mixing a high-temperature biological agent and water-based well drilling solid waste of a sulfonation system by weight of 0.3-1.0 percent: 1 mixing to obtain a first mixture; adding a nutrient accounting for 5-15 wt% of the first mixture to obtain a second mixture; transferring the second mixture to a fermentation tank for treatment for 2-4 days to obtain a third mixture; preparing a normal-temperature biological agent, and mixing the normal-temperature biological agent and the third mixture according to the weight ratio of 0.1-0.5 percent: 1, transferring the mixture to a treatment place, and treating the mixture at 15-35 ℃ for 55-57 days to obtain planting soil; wherein the high-temperature biological agent is bacillus denitrificans thermophilus and/or bacillus calmette guerin, and the normal-temperature biological agent is alcaligenes faecalis phenol subspecies GFB-14. The invention has the advantages of shortening the treatment period of drilling solid waste, not using natural soil, being capable of being used as greening planting soil after solid waste treatment, and the like.
Description
Technical Field
The invention belongs to the technical field of pollution treatment of petroleum and natural gas exploration drilling, and particularly relates to a sulfonation system water-based drilling solid waste biological strengthening treatment process and greening planting soil.
Background
The water-based drilling solid waste is a necessary product of conventional drilling operation of oil and gas exploration, and the production amount in Chongqing areas is generally 0.35-0.4 m3And/m footage. As the geological structure is complex in Chongqing areas and the drilling operation wells are deep, most wells belong to deep wells with the depth of more than 4000m, and well sections at the four-five deep-opening parts are used for preventing and treating underground geologyUnder complex conditions and to ensure rapid and safe drilling, a sulfonated system drilling fluid system is mainly adopted for drilling, and SMP-1 sulfonated phenolic resin, CFK-2, FRH, SD-202, DR-II, RSTF, FK-10, PPL, SP-80, SMT (or TX) and other high polymer drilling fluid treatment agents are added, so that the produced drilling solid waste main pollutant index COD is higher, a large amount of high polymer organic matters are contained, the biodegradation rate is low, but the potassium content is rich, the texture is close to that of loam, and the potential of converting biological treatment into soil for resource utilization is achieved.
The application numbers are: CN201910688639.6, application name: a process for recycling solid wastes of a water-based drilling fluid system; the application numbers are: CN201910361807.0, application name: a microbial treatment process for drilling solid waste and the application number are as follows: CN201910688643.2, application name: a water-based drilling soft solid waste recycling treatment and utilization process; all three patents disclose a method for treating water-based drilling solid waste by combining microorganisms, soil and plants. However, in the actual application process of the method, natural soil with solid waste treatment capacity of 0.5-3 times is required to be added, and natural soil is required to cover the surface layer to plant plants in the treatment process, so that the method has the risks of large workload and damage to the existing ecological environment; there is also a problem that the processing period is long. In view of the above problems, there is a need for a water-based well drilling solid waste recycling process that can further improve the microbial treatment effect, shorten the treatment period, and avoid the synergistic degradation of natural soil and planted plants.
Disclosure of Invention
The present invention aims to address at least one of the above-mentioned deficiencies of the prior art. For example, the invention aims to provide a water-based drilling solid waste recycling process which can further improve the microbial treatment effect, shorten the treatment period and does not use natural soil and planted plants for synergistic degradation.
In order to achieve the purpose, the invention provides a biological enhancement treatment process for water-based drilling solid waste of a sulfonation system, which aims at the water-based drilling solid waste of the sulfonation system with the pollutant content of 4.0-8.0 wt%, the high molecular organic polymer content in the pollutant of 80 wt% and the COD (chemical oxygen demand) of more than or equal to 3000mg/L, and comprises the following steps: preparing a high-temperature biological agent for biologically strengthening the water-based drilling solid waste of the sulfonation system; uniformly mixing the high-temperature biological agent and the water-based drilling solid waste of the sulfonation system according to the weight ratio of 0.3-1.0 wt% to obtain a first mixture; adding a nutrient accounting for 5-15 wt% of the weight of the first mixture, and uniformly mixing to obtain a second mixture; transferring the second mixture into a fermentation tank, and treating for 2-4 days under the conditions that the temperature is 50-70 ℃ and the humidity is 30-35% to obtain a third mixture; preparing a normal-temperature biological agent for biologically strengthening the water-based drilling solid waste of the sulfonation system; uniformly mixing the normal-temperature biological agent and the third mixture according to the weight ratio of 0.1-0.5 wt% to obtain a fourth mixture; transferring the fourth mixture to a treatment place, and treating for 55-57 days under the conditions that the temperature is 15-35 ℃ and the humidity is 22-30% to obtain greening planting soil; wherein the high-temperature biological agent is bacillus denitrificans and/or bacillus xanthus or a bacterial suspension thereof or a culture solution thereof or a fermentation product thereof, and the normal-temperature biological agent is alcaligenes faecalis subsp.
In an exemplary embodiment of the invention, the treatment site may be a treatment tank, a treatment tank or a treatment tank, and the fermentation tank may be a spinning sandwich warming humidity-controlling aerated fermentation tank.
In an exemplary embodiment of the present invention, the preparing the high temperature biological agent includes the steps of: collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site; adding organic matters, various types of water-based drilling fluid additives and a nitrogen source into the sample to obtain a mixture with a carbon-nitrogen ratio of 15-20: 1, adjusting the water content of the mixture to 28-30%, culturing at 50-55 ℃, eliminating strains without degradation capacity, gradually increasing the concentration of each water-based drilling fluid additive, and domesticating to obtain a mixture containing degradation efficient bacteria; separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strains for later use; respectively preparing culture solutions containing one of the multiple types of water-based drilling fluid additives, inoculating the reserved strains in each culture solution, measuring the degradation amount of the water-based drilling fluid additive contained in each culture solution, and selecting the strains with the degradation rate of more than 5% of the water-based drilling fluid additive contained in each culture solution to obtain bacillus denitrificans thermophilus and/or bacillus xanthus; culturing and expanding the thermophilic denitrified bacillus and/or the air-cocklebur bacillus to obtain the high-temperature biological agent.
In an exemplary embodiment of the present invention, the preparing of the normothermic biological agent may include the steps of: collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site; adding organic matters, various types of water-based drilling fluid additives and a nitrogen source into the sample to obtain a mixture with a carbon-nitrogen ratio of 15-20: 1, adjusting the water content of the mixture to 28-30%, culturing at 25-32 ℃, eliminating strains without degradation capacity, gradually increasing the concentration of each water-based drilling fluid additive, and domesticating to obtain a mixture containing degradation efficient bacteria; separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strains for later use; respectively preparing culture solutions containing one of the multiple types of water-based drilling fluid additives, inoculating the reserved strains in each culture solution, measuring the degradation amount of the water-based drilling fluid additives contained in each culture solution, and selecting the strains with the degradation rate of more than 5% of the water-based drilling fluid additives in each culture solution to obtain alcaligenes faecalis phenol subspecies; and culturing and expanding the alcaligenes faecalis phenol subspecies to obtain the normal-temperature biological agent.
In an exemplary embodiment of the invention, the leachate of the greening planting soil has COD less than 100mg/L, germination index more than 80%, organic matter content more than 50g/kg, available nitrogen more than 100mg/kg, available phosphorus more than 85mg/kg and quick-acting potassium more than 9000 mg/kg.
In an exemplary embodiment of the invention, the various types of water-based drilling fluid additives may include sulfonated lignite, sulfonated phenolic resins, carboxymethyl cellulose and potassium polyacrylate.
In an exemplary embodiment of the invention, the separation and purification of the mixture containing the degradation bacterium is performed by dilution plating and plate drawing.
In an exemplary embodiment of the present invention, the separating and purifying the mixture containing the degrading bacteria comprises: oscillating and uniformly mixing the mixture containing the degradation efficient bacteria, and diluting the mixture according to different proportions to obtain diluted solutions with different concentrations; respectively coating the diluted solutions with different concentrations on different beef extract peptone plates, and culturing at constant temperature of 28-30 ℃ for 24-36 hours; and (3) inoculating the bacterial colonies formed on different beef extract peptone plates onto a new beef extract peptone plate, and continuously streaking and separating until bacterial colonies with consistent bacterial colonies and thallus characteristics are obtained, thereby completing separation and purification.
In an exemplary embodiment of the invention, the composition of the broth containing the single aqueous-based drilling fluid additive may include: 0.4-0.6 g/L of single water-based drilling fluid additive, NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003-0.005 g/L, and the pH value of the culture solution containing the single water-based drilling fluid additive is 7.0-7.2.
The invention further provides greening planting soil which can be obtained through the sulfonation system water-based drilling waste fixation biological strengthening treatment process, and the greening planting soil can comprise 100-120 g/kg of organic matters, 90-100 mg/kg of available phosphorus, 9000-10000 mg/kg of available potassium, 130-170 mg/kg of available nitrogen and the balance of loam.
Compared with the prior art, the beneficial effects of the invention can comprise at least one of the following:
(1) compared with the existing drilling solid waste combined treatment process of microorganisms, soil and plants, the sulfonation system water-based drilling solid waste biological strengthening treatment process reduces the links of adding natural soil and covering and planting plants, has simpler process steps and reduces the risk of damaging the natural environment;
(2) the biological strengthening treatment process for the water-based drilling solid waste of the sulfonation system is simple in treatment process, pretreatment such as coagulation, aeration oxygenation, pH regulation, temperature regulation and the like is not needed for the drilling waste slurry, and field operation and management are facilitated;
(3) the biological enhancement treatment process for the water-based drilling solid waste of the sulfonation system adds a high-temperature biological enhancement treatment link, the standard reaching period of the treatment is less than or equal to 60 days, and compared with the existing drilling solid waste treatment process combining microorganisms, soil and plants, the treatment period is shortened by 60-90 days.
Detailed Description
Hereinafter, the water-based drilling solid waste bio-augmentation treatment process and the planting green soil of the sulfonation system of the present invention will be described in detail with reference to the exemplary embodiments.
The invention provides a biological strengthening treatment process for water-based drilling solid waste of a sulfonation system.
In an exemplary embodiment of the invention, the biological strengthening treatment process of the water-based drilling solid waste of the sulfonation system aims at the water-based drilling solid waste of the sulfonation system, wherein the content of the pollutants is 4.0-8.0 wt%, the content of the macromolecular organic polymers in the pollutants is more than 80 wt%, and COD is more than or equal to 3000 mg/L. Specifically, the sulfonated drilling fluid system (comprising potassium polysulfonate drilling fluid, polysulfonate drilling fluid and the like) has high pollutant content, COD (chemical oxygen demand) is generally higher than 3000mg/L, and the sulfonated drilling fluid system is mainly composed of high molecular organic polymers (for example, sulfonated phenolic resin SMP-1, potassium polyacrylate K-PMA, FRH, SD-202, DR-II, RSTF, FK-10, span-80, sulfonated lignite SMT and one or more of various high molecular drilling fluid treating agents and the like). The content of the pollutants is based on the mass of the water-based drilling solid waste of the sulfonation system, and the content of the high-molecular organic polymer is based on the mass of the pollutants. The bioaugmentation treatment process may include the steps of:
preparing the high-temperature biological agent for biologically strengthening the water-based drilling solid waste of the sulfonation system. Here, the high-temperature biological agent is Bacillus denitrificans and/or Bacillus xanthus or a bacterial suspension thereof or a culture solution thereof or a fermentation product thereof. By utilizing the characteristics of strong metabolic capability and high propagation speed of the high-temperature biological agent, the quantity of pollutants degrading microorganisms in the treated substances can be increased, the degradation rate of the pollutants is accelerated, and the degradation period is shortened. By adding a high-temperature biological strengthening treatment link, the standard reaching period of the biological strengthening treatment process for the water-based drilling solid waste of the sulfonation system can be less than or equal to 60 days, and compared with the existing drilling solid waste treatment process combining microorganisms, soil and plants, the treatment period is shortened by 60-90 days. In the present exemplary embodiment, the preparing the high temperature biological agent includes the steps of:
and S1, collecting the sample. Collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site. For example, a potassium polysulfonate system water based drilling solid waste sample may be collected from an oil and gas drilling site using sterile sampling bags. The water-based drilling solid waste sample can be old or fresh water-based drilling solid waste of a sulfonation system, soil or water polluted by water-based drilling solid waste of the sulfonation system, and soil or water sample polluted by water-based drilling fluid of the sulfonation system. The sample is the source of the thermophilic denitrified bacillus and/or the ochrobactrum anthropi.
S2, domestication of the thermophilic denitrified bacillus and/or the ochrobactrum pallidum.
And adding a water-based drilling fluid additive, organic matters and a nitrogen source into the collected water-based drilling solid waste sample to obtain a mixture. Adjusting the water content of the mixture to 28-30% by using distilled water and culturing at 50-55 ℃. Under the conditions, the strains capable of degrading the water-based drilling solid waste additive can be propagated in large quantities. Then eliminating microorganisms (strains) without degradation capability, and gradually increasing the concentration of the water-based drilling fluid additive so as to domesticate degradation high-efficiency bacteria with strong degradation capability and strong tolerance to the additive. The organic matter can be easily degraded organic matters such as corn straws and rice bran. The nitrogen source can be a nitrogen fertilizer, and the nitrogen fertilizer can be ammonium bicarbonate, ammonium sulfate, ammonium chloride and the like. The organic matter and the nitrogen fertilizer are added to keep the carbon-nitrogen ratio in the mixture between 15 and 20:1 so as to be beneficial to the growth of the strain, and for example, the carbon-nitrogen ratio can be 18: 1. The water content is controlled to be 28-30 percent, and the temperature is controlled to be 50-55 ℃ so that the strain has better temperature resistance. Too low a water content may result in too dry a growth environment, resulting in hypoxia of the strain. The temperature of the culture is too high or too low to be suitable for the growth of the strain. Further, the water content may be controlled at 29%, and the temperature of the culture may be 52 ℃.
Further, the water-based drilling fluid additive comprises sulfonated lignite, sulfonated phenolic resin, carboxymethyl cellulose (CMC) and potassium polyacrylate (K-PAM).
S3, separating and purifying the bacillus denitrificans thermophilus and/or the bacillus xanthus.
Separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strain for later use. Further, the separated and purified strain is inoculated to a slant culture medium for storage and standby. The vessels and water used in the separation and purification process are 100% sterile.
S4, rescreening the thermophilic denitrified bacillus and/or the ochrobactrum anthropi. Respectively preparing culture solutions containing one water-based drilling fluid additive among a plurality of types of water-based drilling fluid additives (namely, preparing one culture solution corresponding to each water-based drilling fluid additive, wherein each culture solution contains one single type of water-based drilling fluid additive), inoculating the separated and purified strains in each culture solution, measuring the reduction amount of a target substrate of the culture solution (the degradation amount of the water-based drilling fluid additive), and selecting the strains with higher degradation rate on various water-based drilling fluid additives, namely the bacillus denitrificans thermophilus and/or the bacillus xanthus. The obtained Geobacillus thermodenitrificans and/or Bacillus xanthans was inoculated into a slant medium and stored at 4 ℃. Strains with degradation rates greater than 5%, for example, greater than 7% may be selected in the selection of the additive for each of the water-based drilling fluids.
When the water-based drilling fluid additive is sulfonated lignite, sulfonated phenolic resin, carboxymethyl cellulose (CMC) and potassium polyacrylate (K-PAM), the culture solution of the water-based drilling fluid additive can be a culture solution containing the sulfonated lignite, a culture solution containing the sulfonated phenolic resin, a culture solution containing the carboxymethyl cellulose and a culture solution containing the potassium polyacrylate respectively. The strain with higher degradation rate to the 4 additives is selected to obtain the bacillus denitrificans thermophilus and/or the bacillus calmette-guerin.
In this embodiment, the separation and purification method may adopt a dilution plating method and a plate-scribing method for separation and purification. Further, the method for separation and purification may include:
and S10, oscillating and uniformly mixing the mixture containing the degradation efficient bacteria, and diluting according to different proportions to obtain diluted solutions with different concentrations. For example, the mixture containing the degradation effective bacteria is put into a triangular flask with sterile water and shaken to mix evenly. The concentration range of the different-concentration diluted solution can be selected from a mass concentration range of 0.1-4%.
S20, coating the diluted solutions with different concentrations on different beef extract peptone plates respectively, and culturing at 50-55 ℃ for 24-36 hours at constant temperature of 52 ℃ for example.
And S30, inoculating the bacterial colonies formed on different beef extract peptone plates onto new beef extract peptone plates, continuously streaking and separating until bacterial colonies with consistent bacterial characteristics are obtained, and completing separation and purification.
In this exemplary embodiment, the broth containing the single aqueous-based drilling fluid additive may include:
the culture solution containing the sulfonated phenolic resin comprises: 0.4-0.6 g/L of sulfonated phenolic resin and NaHPO40.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, 0.5g/L of sulfonated phenolic resin, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
For the culture solution containing sulfonated lignite: 0.4-0.6 g/L of sulfonated lignite, NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, sulfonated lignite 0.5g/L, NaHPO40.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL20.004g/L。
The culture solution containing carboxymethyl cellulose comprises: 0.4-0.6 g/L of carboxymethyl cellulose, NaHPO40.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, carboxymethyl cellulose 0.5g/L, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
The culture solution containing potassium polyacrylate comprises: 0.4-0.6 g/L of potassium polyacrylate and NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, potassium polyacrylate 0.5g/L, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
As described above, the pH of the culture medium containing each additive may be 7.0 to 7.2. Sterilizing each culture medium at 121 ℃ for 20-30 min.
In this example, the beef extract peptone medium and the slant medium may include: 10g/L of peptone, 5g/L of sodium chloride and 5g/L of beef extract. The pH value of the slant culture medium can be 7.0-7.2, and the culture medium can be prepared after sterilization for 30min at 121 ℃. Of course, other kinds of separation media can be selected in the separation and purification process of the strain of the invention to replace the beef extract peptone medium.
In this embodiment, the thermophilic denitrifying bacillus and/or Bacillus xanthans is aerobic, and further is strictly aerobic, and can be used only under aerobic conditions.
In this embodiment, the Geobacillus thermodenitrificans and/or Bacillus xanthus may be grown at a pH of 5.0 to 10.0, a salt concentration of 0% to 4%, and a temperature of 15 to 70 ℃. Furthermore, the growth can be carried out under the conditions that the pH is 6.5-7.5, the temperature is 50-55 ℃, and the salt concentration is 0.5-1%.
In this example, the colony formed after culturing the bacillus denitrificans and/or bacillus pallidus on a beef extract peptone medium for 24 hours is round or irregular, and the colony after 48 hours is round, milky white (white), 2-3 mm in diameter, irregular in edge, and flat and moist. In the above way, the thermophilic denitrifying bacillus and/or the bacillus xanthil can degrade various drilling fluid additives such as sulfonated lignite, sulfonated phenolic resin, PMA, CMC and the like at the same time, and solves the problem that the separated bacterial strain can only degrade a single substance and needs to use a compound bacterial system to treat pollutants. The separated strain has strong alkali resistance and salt resistance, can play a role in degrading the drilling solid waste additive under the conditions that the pH is 5.0-10.0 and the salt concentration is 0-4%, and has a wide growth temperature range (15-70 ℃). And the thermophilic denitrogenation bacillus and/or the ochrobactrum anthropi can repair the medium polluted by the solid waste of the water-based drilling fluid, wherein the medium is soil, water and air. The bacillus thermodenitrificans and/or bacillus air pallidus can degrade Total Organic Carbon (TOC) of water-based drilling solid waste. The strain obtained by domestication and separation is used as a biological preparation for biological enhancement treatment of water-based drilling solid waste of a sulfonation system after being cultured and expanded. Here, the culture expansion includes the steps of: the obtained strain is inoculated in a beef extract peptone liquid medium for activation for 16 hours, and is inoculated in an amplification medium (5 g of peptone, 10g of sucrose, 1g of beef extract, 10g of sodium chloride, 1L, pH 7.0.0 of tap water, sterilization at 121 ℃ for 30min) according to the inoculation amount of 5 percent,50-55 ℃ and air flow of 1.2L/min-1When the OD600 of the bacterial liquid reaches 1.0, the bacterial liquid is the liquid biological preparation; the liquid biological agent is mixed into the organic fertilizer according to 25 percent to prepare the solid biological agent.
In the embodiment, a nutrient accounting for 5-15 wt% of the weight of the first mixture is added and uniformly mixed to obtain a second mixture. And transferring the second mixture into a fermentation tank, and treating for 2-4 days at the temperature of 50-70 ℃, the rotating speed of 2-4 r/h and the interval of 12h under the conditions of aeration and oxygenation and the humidity of 30-35% to obtain a third mixture. Here, the fermentation tank may be a self-rotating sandwich warming humidity-controlling aeration fermentation tank. The high-temperature degradation bacteria mainly degrade pollutants such as petroleum hydrocarbon, sulfonated lignite, sulfonated phenolic resin, PMA, CMC and the like. After the high-temperature stage treatment, the pollutants (COD content in the leaching solution) are degraded to more than 70%.
In the present exemplary embodiment, a normal temperature biological agent for biological enhanced treatment of water-based drilling solid waste of a sulfonation system is prepared. Wherein the biological agent is Alcaligenes faecalis subspecies GFB-14 or bacterial suspension thereof or culture solution thereof or fermentation product thereof. However, the present invention is not limited thereto, and other microorganisms having the same or similar functions, for example, Brevundimonas GFB-6, Bacillus pumilus GFB-8, etc. may be used. Specifically, most pollutants are degraded after the water-based drilling solid waste of the sulfonation system is degraded at high temperature, and the rest pollutants (about 0-30%) are degraded by using a normal-temperature biological agent. The normal-temperature degradation microbial inoculum is used for treating part of undegraded pollutants, so that the treatment cost can be reduced, and the economic benefit can be improved.
In the present exemplary embodiment, the preparing the biological agent may include the steps of:
and S01, collecting the sample. Collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site. For example, a potassium polysulfonate system water based drilling solid waste sample may be collected from an oil and gas drilling site using sterile sampling bags. The water-based drilling solid waste sample can be old or fresh water-based drilling solid waste of a sulfonation system, soil or water polluted by water-based drilling solid waste of the sulfonation system, and soil or water sample polluted by water-based drilling fluid of the sulfonation system. The sample is the source of Alcaligenes faecalis subsp.
S02, domestication of Alcaligenes faecalis subsp.
And adding a water-based drilling fluid additive, organic matters and a nitrogen source into the collected water-based drilling solid waste sample to obtain a mixture. Regulating the water content of the mixture to 28-30% by using distilled water and culturing at 25-32 ℃. Under the conditions, the strains capable of degrading the water-based drilling solid waste additive can be propagated in large quantities. Then eliminating microorganisms (strains) without degradation capability, and gradually increasing the concentration of the water-based drilling fluid additive so as to domesticate degradation high-efficiency bacteria with strong degradation capability and strong tolerance to the additive. The organic matter can be easily degraded organic matters such as corn straws and rice bran. The nitrogen source can be a nitrogen fertilizer, and the nitrogen fertilizer can be ammonium bicarbonate, ammonium sulfate, ammonium chloride and the like. The organic matter and the nitrogen fertilizer are added to keep the carbon-nitrogen ratio in the mixture between 15 and 20:1 so as to be beneficial to the growth of the strain, and for example, the carbon-nitrogen ratio can be 18: 1. The water content is controlled to be 28-30 percent, and the temperature is controlled to be 25-32 ℃ so that the strain has a better biological production environment. Too low a water content may result in too dry a growth environment, resulting in hypoxia of the strain. The temperature of the culture is too high or too low to be suitable for the growth of the strain. Further, the water content may be controlled at 29%, and the temperature of the culture may be 30 ℃.
Further, the water-based drilling fluid additive comprises sulfonated lignite, sulfonated phenolic resin, carboxymethyl cellulose (CMC) and potassium polyacrylate (K-PAM).
S03, and separating and purifying Alcaligenes faecalis subsp.
Separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strain for later use. Further, the separated and purified strain is inoculated to a slant culture medium for storage and standby. The vessels and water used in the separation and purification process are 100% sterile.
S04, carrying out secondary screening on Alcaligenes faecalis subsp.
Respectively preparing culture solution containing one water-based drilling fluid additive in a plurality of types of water-based drilling fluid additives (namely, each water-based drilling fluid additive correspondingly prepares one culture solution, each culture solution contains a single type of water-based drilling fluid additive), inoculating the separated and purified strains in each culture solution, measuring the reduction amount of a target substrate of the culture solution (the degradation amount of the water-based drilling fluid additive), and selecting the strains with higher degradation rate to the various water-based drilling fluid additives, namely Alcaligenes faecalis subsp. The obtained Alcaligenes faecalis subsp.Phenoleicus GFB-14 was inoculated to a slant medium and stored at 4 ℃. Strains with degradation rates greater than 5%, for example, greater than 7% may be selected in the selection of the additive for each of the water-based drilling fluids.
When the water-based drilling fluid additive is sulfonated lignite, sulfonated phenolic resin, carboxymethyl cellulose (CMC) and potassium polyacrylate (K-PAM), the culture solution of the water-based drilling fluid additive can be a culture solution containing the sulfonated lignite, a culture solution containing the sulfonated phenolic resin, a culture solution containing the carboxymethyl cellulose and a culture solution containing the potassium polyacrylate respectively. The strain with higher degradation rate to the 4 additives is selected to obtain Alcaligenes faecalis subsp.
In this embodiment, the separation and purification method may adopt a dilution plating method and a plate-scribing method for separation and purification. Further, the method for separation and purification may include:
s100, oscillating and uniformly mixing the mixture containing the degradation efficient bacteria, and diluting according to different proportions to obtain diluted solutions with different concentrations. For example, the mixture containing the degradation effective bacteria is put into a triangular flask with sterile water and shaken to mix evenly. The concentration range of the different-concentration diluted solution can be selected from a mass concentration range of 0.1-4%.
S200, respectively coating the diluted solutions with different concentrations on different beef extract peptone plates, and culturing at the constant temperature of 28-30 ℃, for example, at 29 ℃ for 24-36 hours.
S300, inoculating the bacterial colonies formed on different beef extract peptone plates onto new beef extract peptone plates, continuously streaking and separating until bacterial colonies with consistent bacterial colonies and thallus characteristics are obtained, and completing separation and purification.
In exemplary embodiments of the invention, the broth containing the single aqueous-based drilling fluid additive may include:
the culture solution containing the sulfonated phenolic resin comprises: 0.4-0.6 g/L of sulfonated phenolic resin and NaHPO40.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, 0.5g/L of sulfonated phenolic resin, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
For the culture solution containing sulfonated lignite: 0.4-0.6 g/L of sulfonated lignite, NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, sulfonated lignite 0.5g/L, NaHPO40.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL20.004g/L。
The culture solution containing carboxymethyl cellulose comprises: 0.4-0.6 g/L of carboxymethyl cellulose, NaHPO40.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl2 0.003g/L～0.005g/L. Further, carboxymethyl cellulose 0.5g/L, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
The culture solution containing potassium polyacrylate comprises: 0.4-0.6 g/L of potassium polyacrylate and NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003g/L to 0.005 g/L. Further, potassium polyacrylate 0.5g/L, NaHPO4 0.2g/L，KH2PO41.0g/L, ammonium acetate 0.3g/L, MgSO4·7H2O 0.5g/L，MnSO4·H2O 0.04g/L，CaCL2 0.004g/L。
As described above, the pH of the culture medium containing each additive may be 7.0 to 7.2. Sterilizing each culture medium at 121 ℃ for 20-30 min.
In this example, the beef extract peptone medium and the slant medium may include: 10g/L of peptone, 5g/L of sodium chloride and 5g/L of beef extract. The pH value of the slant culture medium can be 7.0-7.2, and the culture medium can be prepared after sterilization for 30min at 121 ℃. Of course, other kinds of separation media can be selected in the separation and purification process of the strain of the invention to replace the beef extract peptone medium.
In this embodiment, the Alcaligenes faecalis subsp. phenoolicus GFB-14 is of an aerobic type, and further of a strictly aerobic type, and can be used only under aerobic conditions.
In this embodiment, the Alcaligenes faecalis subsp. Phenolicum GFB-14 can grow under the conditions of pH 5.0-10.0, salt concentration 0% -4%, and temperature 15-45 ℃. Furthermore, the growth can be carried out under the conditions that the pH is 6.5-7.5, the temperature is 28-35 ℃, and the salt concentration is 0.5-1%.
In this example, the colony formed by culturing Alcaligenes faecalis subsp. Phenolicus GFB-14 on a beef extract peptone medium for 24 hours is circular or irregular, and the colony formed after 48 hours is circular, milky white (white), 2-3 mm in diameter, irregular in edge, and flat and wet.
In the invention, the Alcaligenes faecalis subsp.Phenolocus GFB-14 can degrade various drilling fluid additives such as sulfonated lignite, sulfonated phenolic resin, PMA and CMC simultaneously, and solves the problem that the separated bacterial strain can only degrade a single substance and needs to use a compound bacterial system to treat pollutants. The separated strain has strong alkali resistance and salt resistance, can play a role in degrading the drilling solid waste additive under the conditions that the pH is 5.0-10.0 and the salt concentration is 0-4 percent, and has a wide growth temperature range (15-45 ℃). And the alcaligenes faecalis phenol subspecies can repair the medium polluted by the solid waste of the water-based drilling fluid, wherein the medium is soil, water and air. The Alcaligenes faecalis subsp. Phenolicum GFB-14 can degrade Total Organic Carbon (TOC) of water-based drilling solid waste. The strain obtained by domestication and separation is used as a normal temperature biological agent for biological strengthening treatment of water-based drilling solid waste of a sulfonation system after being cultured and expanded. Here, the culture expansion includes the steps of: inoculating the obtained strain into beef extract peptone liquid culture medium, activating for 16 hr, inoculating into an amplification culture medium (peptone 5g, sucrose 10g, beef extract 1g, sodium chloride 10g, tap water 1L, pH 7.0.0, sterilizing at 121 deg.C for 30min) at a inoculation amount of 5%, sterilizing at 28-35 deg.C and ventilation amount of 1.2 L.min-1When the OD600 of the bacterial liquid reaches 1.0, the bacterial liquid is the liquid biological preparation; the liquid biological agent is mixed into the organic fertilizer according to 25 percent to prepare the solid biological agent.
In the exemplary embodiment, the normal temperature biological agent and the third mixture are uniformly mixed according to a weight ratio of 0.1-0.5 wt% to obtain a fourth mixture. And transferring the fourth mixture to a treatment place, and treating for 55-57 days at 15-35 ℃ and under the humidity of 22% -30% to obtain the greening planting soil. Specifically, the high-temperature biological agent prepared in the steps and the water-based drilling solid waste of the sulfonation system to be treated are mixed according to the weight ratio of 0.3-1.0 wt%: 1, mixing uniformly. And transferring the fourth mixture to a treatment place, and treating the fourth mixture for 55-57 days at the temperature of 15-35 ℃ and the humidity of 22-30% to obtain the greening planting soil, wherein the treatment period is shortened by 60-90 days compared with the existing drilling solid waste treatment process by combining microorganisms, soil and plants. The treatment site may be a treatment tank, a treatment tank or a treatment tank. The nutrient may include at least one of organic fertilizer, straw, bran and rice bran. The COD of the green planting soil leachate is less than 100mg/L, the germination index is more than 80%, the organic matter content is more than 50g/kg, the available nitrogen is more than 100mg/kg, the available phosphorus is more than 85mg/kg, and the available potassium is more than 9000 mg/kg. The range meets the requirements of the standards of CJ/T340-2016 III for greening planting soil.
The invention also provides greening planting soil.
In another exemplary embodiment of the invention, the planting soil can be obtained by the sulfonation system water-based drilling waste fixation biological enhancement treatment process of the above one exemplary embodiment, and the planting soil can comprise 100-120 g/kg of organic matter, 90-100 mg/kg of available phosphorus, 9000-10000 mg/kg of available potassium, 130-170 mg/kg of available nitrogen, and the balance of loam.
In summary, the beneficial effects of the invention can include at least one of the following:
(1) compared with the existing drilling solid waste combined treatment process of microorganisms, soil and plants, the sulfonation system water-based drilling solid waste biological strengthening treatment process reduces the links of adding natural soil and covering and planting plants, has simpler process steps and reduces the risk of damaging the natural environment;
(2) the biological strengthening treatment process for the water-based drilling solid waste of the sulfonation system is simple in treatment process, pretreatment such as coagulation, aeration oxygenation, pH regulation, temperature regulation and the like is not needed for the drilling waste slurry, and field operation and management are facilitated;
(3) the biological enhancement treatment process for the water-based drilling solid waste of the sulfonation system adds a high-temperature biological enhancement treatment link, the standard reaching period of the treatment is less than or equal to 60 days, and compared with the existing drilling solid waste treatment process combining microorganisms, soil and plants, the treatment period is shortened by 60-90 days.
While the present invention has been described above in connection with exemplary embodiments, it will be apparent to those of ordinary skill in the art that various modifications may be made to the above-described embodiments without departing from the spirit and scope of the claims.
Claims (10)
1. A biological strengthening treatment process for water-based drilling solid waste of a sulfonation system is characterized by comprising the following steps of:
preparing a high-temperature biological agent for biologically strengthening the water-based drilling solid waste of the sulfonation system;
uniformly mixing the high-temperature biological agent and the water-based drilling solid waste of the sulfonation system according to the weight ratio of 0.3-1.0 wt% to obtain a first mixture;
adding a nutrient accounting for 5-15 wt% of the weight of the first mixture, and uniformly mixing to obtain a second mixture;
transferring the second mixture into a fermentation tank, and treating for 2-4 days under the conditions that the temperature is 50-70 ℃ and the humidity is 30-35% to obtain a third mixture;
preparing a normal-temperature biological agent for biologically strengthening the water-based drilling solid waste of the sulfonation system;
uniformly mixing the normal-temperature biological agent and the third mixture according to the weight ratio of 0.1-0.5 wt% to obtain a fourth mixture;
transferring the fourth mixture to a treatment place, and treating for 55-57 days under the conditions that the temperature is 15-35 ℃ and the humidity is 22-30% to obtain greening planting soil;
wherein the high-temperature biological agent is bacillus denitrificans and/or bacillus xanthus or a bacterial suspension thereof or a culture solution thereof or a fermentation product thereof, and the normal-temperature biological agent is alcaligenes faecalis subsp.
2. The biologically enhanced treatment process for the solid waste of the sulfonated system water-based well drilling as claimed in claim 1, wherein the treatment site is a treatment tank, a treatment tank or a treatment box, and the fermentation tank is a self-rotating sandwich heating humidity-controlling aeration fermentation tank.
3. The biological enhancement treatment process for the solid waste of the sulfonated system water-based well drilling as claimed in claim 1, wherein the preparation of the high temperature biological agent comprises the steps of:
collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site;
adding organic matters, various types of water-based drilling fluid additives and a nitrogen source into the sample to obtain a mixture with a carbon-nitrogen ratio of 15-20: 1, adjusting the water content of the mixture to 28-30%, culturing at 50-55 ℃, eliminating strains without degradation capacity, gradually increasing the concentration of each water-based drilling fluid additive, and domesticating to obtain a mixture containing degradation efficient bacteria;
separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strains for later use;
respectively preparing culture solutions containing one of the multiple types of water-based drilling fluid additives, inoculating the reserved strains in each culture solution, measuring the degradation amount of the water-based drilling fluid additive contained in each culture solution, and selecting the strains with the degradation rate of more than 5% of the water-based drilling fluid additive contained in each culture solution to obtain bacillus denitrificans thermophilus and/or bacillus xanthus;
culturing and expanding the thermophilic denitrified bacillus and/or the air-cocklebur bacillus to obtain the high-temperature biological agent.
4. The biological enhancement treatment process for the solid waste of the sulfonated system water-based drilling well according to claim 1, wherein the preparation of the normal temperature biological agent comprises the steps of:
collecting a potassium polysulfonate system water-based drilling solid waste sample from an oil and gas drilling well site;
adding organic matters, various types of water-based drilling fluid additives and a nitrogen source into the sample to obtain a mixture with a carbon-nitrogen ratio of 15-20: 1, adjusting the water content of the mixture to 28-30%, culturing at 25-32 ℃, eliminating strains without degradation capacity, gradually increasing the concentration of each water-based drilling fluid additive, and domesticating to obtain a mixture containing degradation efficient bacteria;
separating and purifying the mixture containing the degradation high-efficiency bacteria, and storing the separated and purified bacterial strains for later use;
respectively preparing culture solutions containing one of the multiple types of water-based drilling fluid additives, inoculating the reserved strains in each culture solution, measuring the degradation amount of the water-based drilling fluid additives contained in each culture solution, and selecting the strains with the degradation rate of more than 5% of the water-based drilling fluid additives in each culture solution to obtain alcaligenes faecalis phenol subspecies;
and culturing and expanding the alcaligenes faecalis phenol subspecies to obtain the normal-temperature biological agent.
5. The biological enhancement treatment process for the water-based drilling solid waste of the sulfonation system according to claim 4, wherein the leachate of the planting green soil is COD less than 100mg/L, the germination index is more than 80%, the organic matter content is more than 50g/kg, the available nitrogen is more than 100mg/kg, the available phosphorus is more than 85mg/kg, and the available potassium is more than 9000 mg/kg.
6. The process of claim 3 or 4, wherein the various types of water-based drilling fluid additives comprise sulfonated lignite, sulfonated phenolic resins, carboxymethyl cellulose and potassium polyacrylate.
7. The biological enhancement treatment process for the sulfonated system water-based drilling solid waste according to claim 3 or 4, wherein the mixture containing the degradation highly effective bacteria is separated and purified by a dilution plate coating method and a plate marking method.
8. The biological enhancement treatment process for the solid waste in the water-based drilling of the sulfonation system as claimed in claim 3 or 4, wherein the separation and purification of the mixture containing the degradation highly effective bacteria comprises:
oscillating and uniformly mixing the mixture containing the degradation efficient bacteria, and diluting the mixture according to different proportions to obtain diluted solutions with different concentrations;
respectively coating the diluted solutions with different concentrations on different beef extract peptone plates, and culturing at constant temperature of 28-30 ℃ for 24-36 hours;
and (3) inoculating the bacterial colonies formed on different beef extract peptone plates onto a new beef extract peptone plate, and continuously streaking and separating until bacterial colonies with consistent bacterial colonies and thallus characteristics are obtained, thereby completing separation and purification.
9. The biological enhancement treatment process for the solid waste of the sulfonated system water-based well drilling as claimed in claim 4, wherein the culture solution containing the single water-based drilling fluid additive comprises the following components: 0.4-0.6 g/L of single water-based drilling fluid additive, NaHPO4 0.1g/L～0.3g/L，KH2PO40.9-1.1 g/L, 0.2-0.4 g/L ammonium acetate, MgSO4·7H2O 0.4g/L～0.6g/L，MnSO4·H2O 0.03g/L～0.05g/L，CaCl20.003-0.005 g/L, and the pH value of the culture solution containing the single water-based drilling fluid additive is 7.0-7.2.
10. The greening planting soil is obtained by the biological enhanced treatment process of water-based drilling solid waste of the sulfonation system as claimed in any one of claims 1 to 9, and comprises 100 to 120g/kg of organic matter, 90 to 100mg/kg of available phosphorus, 9000 to 10000mg/kg of quick-acting potassium, 130 to 170mg/kg of available nitrogen and the balance of loam.
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