CN112772927A - Probiotic freeze-dried powder - Google Patents
Probiotic freeze-dried powder Download PDFInfo
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- CN112772927A CN112772927A CN202011638123.XA CN202011638123A CN112772927A CN 112772927 A CN112772927 A CN 112772927A CN 202011638123 A CN202011638123 A CN 202011638123A CN 112772927 A CN112772927 A CN 112772927A
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- fixedly connected
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- dried powder
- probiotic
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- 239000000843 powder Substances 0.000 title claims abstract description 61
- 239000006041 probiotic Substances 0.000 title claims abstract description 51
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 51
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 50
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 18
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 18
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000011782 vitamin Substances 0.000 claims abstract description 11
- 235000013343 vitamin Nutrition 0.000 claims abstract description 11
- 229940088594 vitamin Drugs 0.000 claims abstract description 11
- 229930003231 vitamin Natural products 0.000 claims abstract description 11
- 239000005862 Whey Substances 0.000 claims abstract description 10
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 9
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 9
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 9
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims abstract description 9
- 229960003080 taurine Drugs 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 claims abstract description 8
- 235000021119 whey protein Nutrition 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 43
- 238000000855 fermentation Methods 0.000 claims description 18
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 12
- 238000004108 freeze drying Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 4
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 241000186869 Lactobacillus salivarius Species 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 238000010030 laminating Methods 0.000 claims description 3
- 210000000214 mouth Anatomy 0.000 claims description 3
- 239000003223 protective agent Substances 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000009833 condensation Methods 0.000 description 5
- 230000005494 condensation Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 235000013325 dietary fiber Nutrition 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 230000037353 metabolic pathway Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229920005560 fluorosilicone rubber Polymers 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B25/00—Details of general application not covered by group F26B21/00 or F26B23/00
- F26B25/04—Agitating, stirring, or scraping devices
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/04—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
- F26B5/06—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/181—Salivarius
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Drying Of Solid Materials (AREA)
Abstract
The invention discloses probiotic freeze-dried powder which is prepared from the following components in parts by weight: 30-40 parts of modified probiotic freeze-dried powder, 5-10 parts of stachyose, 5-15 parts of desalted whey powder, 5-15 parts of whey protein concentrate powder, 0.5-0.9 part of edible essence, 1-6 parts of prebiotics, 0.01-0.1 part of taurine, 0.5-1 part of mixed vitamins and 2-4 parts of trehalose.
Description
Technical Field
The invention relates to the technical field of freeze-dried powder preparation, and particularly relates to probiotic freeze-dried powder.
Background
The freeze-dried powder is prepared by freezing the water in the liquid medicine in advance by adopting a vacuum freeze-drying method of a freeze dryer, and then sublimating the frozen water in the liquid medicine in a vacuum sterile environment, thereby obtaining freeze drying. In short, the water in the liquid medicine is pumped out in a low-temperature environment, and the original medicinal effect is kept.
The digestion and absorption of food are important components of human health, and compared with the pore structure of human small intestine villi, various foods after chewing are also macromolecules, and probiotics are needed to help decompose into small molecules to be digested and absorbed. If these nutrients are decomposed by harmful bacteria, an offensive odor and toxins are generated. The odor and peculiar smell generated by decomposition of harmful bacteria mainly comprise volatile gases such as ammonia gas, methane, hydrogen sulfide and the like.
The probiotic freeze-dried powder is in a constant state in the process of vacuum freeze-drying equipment, the condensation speed of water in the material is low, the material is not favorable for quickly separating out water in the material, the vacuum freeze-drying speed is low, and the vacuum freeze-drying is not favorable for carrying out vacuum freeze-drying.
Therefore, a probiotic freeze-dried powder is provided to solve the above problems.
Disclosure of Invention
The invention aims to provide probiotic freeze-dried powder to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the probiotic freeze-dried powder is prepared from the following components in parts by weight: 30-40 parts of modified probiotic freeze-dried powder, 5-10 parts of stachyose, 5-15 parts of desalted whey powder, 5-15 parts of whey protein concentrate powder, 0.5-0.9 part of edible essence, 1-6 parts of prebiotics, 0.01-0.1 part of taurine, 0.5-1 part of mixed vitamins and 2-4 parts of trehalose.
A preparation process for probiotic freeze-dried powder comprises the following steps:
respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing for 32-38h at 35-40 ℃, inoculating a test tube strain into a triangular flask liquid MRS culture medium, fermenting and culturing for 36-40h at 42-45 ℃;
step two, preparing a fermentation culture medium by taking modified probiotic freeze-dried powder, stachyose, desalted whey powder, whey protein concentrate powder, edible essence, prebiotics, taurine, mixed vitamins and trehalose as raw materials, inoculating a strain expanding solution according to the inoculation amount of 3-5% (v/v) after sterilizing the fermentation culture medium, carrying out sealed culture at 30-37 ℃ for 1-2 days, stopping fermentation when the pH value is reduced to 3.5-4.0, and carrying out anaerobic fermentation at the fermentation temperature of 35-40 ℃;
thirdly, centrifuging the fermented probiotic fermentation liquor at a high speed at the revolution of 8000-;
and step four, adding the obtained oral cavity probiotic bacteria mud into a protective agent to embed probiotic bacteria, and putting the embedded probiotic bacteria into a freeze-drying machine to obtain freeze-dried powder after freeze drying.
The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder comprises a bottom plate, a door plate, a hinge, an observation window, a controller, a box body, a lock catch and a material plate, wherein the top of the bottom plate is fixedly connected with the box body, an accelerating structure and a vacuumizing structure;
the vacuumizing structure comprises a transverse pipe, a straight pipe, an air outlet pipe, an electromagnetic valve and an air pump, wherein the air outlet pipe is fixedly connected to the output end of the air pump, the straight pipe is fixedly connected to the input end of the air pump, the electromagnetic valve is fixedly connected to the bottom of the straight pipe, and the straight pipe is uniformly and fixedly connected with the transverse pipe for air pumping;
the accelerating structure comprises a first belt pulley, a second belt pulley, a driving motor, a belt, a rotating rod, a straight groove, a taper ruler, a transverse plate, a sliding plate, a threaded groove, a cam, a spring and a movable plate, wherein the output end of the driving motor is fixedly connected with the second belt pulley, the second belt pulley is uniformly and movably connected with the first belt pulley, the first belt pulley is fixedly connected with the rotating rod, the threaded groove is formed in the rotating rod at the position in the box body, the cam is respectively and fixedly connected with the rotating rod at the two ends of the threaded groove, the sliding plate is connected with the first belt pulley through the threaded groove and the threaded groove, the straight groove is formed in the inner bottom of the sliding plate, the spring is uniformly and fixedly connected with the inner top of the straight groove, the movable plate is fixedly connected with the bottom of the spring, the outer wall of the movable plate is in, the bottom of the transverse plate is uniformly and fixedly connected with conical rulers, and the conical rulers are attached to the inner top of the material plate in a sliding manner;
the box body is fixedly and uniformly connected with a condensing pipe for refrigeration at the position below the first belt pulley, and the inner wall of the box body is fixedly connected with a temperature sensor for temperature detection;
the box body is connected with an installation supporting structure used for installing and supporting the material plate, the installation supporting structure comprises a limiting sliding groove, a U-shaped plate, a C-shaped supporting plate, a limiting sliding block and a connecting block, the limiting sliding groove is formed in the rear side wall of the box body, the limiting sliding block is attached and slidably connected in the limiting sliding groove, the connecting block is fixedly connected to the front side wall of the limiting sliding block at the position corresponding to the rotating rod, the U-shaped plate is fixedly connected to the bottom of the connecting block, the top of the material plate is attached and slidably connected with the top of the U-shaped plate, the C-shaped supporting plate is fixedly connected to the bottom of the U-shaped plate, and the;
the flitch is connected with the fixed knot who is used for flitch and U-shaped board fixed connection and constructs, fixed knot constructs including cross slot, spring, straight piece and L shape pole, the top front end at the flitch is seted up to the cross slot, the laminating sliding connection has L shape pole in the cross slot, the flitch is at the straight piece of the bottom outer end fixedly connected with of cross slot, the inner wall fixedly connected with spring of straight piece, the inner end of spring and the position fixed connection that stands vertically of L shape pole, the horizontal position of L shape pole is pegged graft with the front end jack of C shape backup pad.
Furthermore, the electromagnetic valve is fixedly installed on the bottom plate through a fixing bolt.
Furthermore, the side wall of the box body is rotatably connected with the rotating rod through a bearing fixedly connected with the side wall, and the transverse pipes are in one-to-one correspondence with the rotating rod.
Furthermore, the rear end of the transverse plate is attached to the inner wall of the material plate in a sliding mode.
Furthermore, the driving motor is fixedly connected with the bottom plate through a mounting frame fixedly connected with the driving motor.
Furthermore, the limiting sliding block is in a dovetail shape, an L shape or a T shape.
Further, the C-shaped supporting plate is connected with the cam in a sliding mode.
Furthermore, the fixing structures are symmetrically arranged at the front ends of the material plates.
The invention has the beneficial effects that:
according to the invention, the driving motor of the accelerating structure drives the second belt pulley to rotate, the second belt pulley drives the belt to move, the belt drives the first belt pulley to rotate, the first belt pulley drives the rotating rod to rotate, the rotating rod drives the sliding plate to move through the threaded groove, the sliding plate drives the movable plate to move, the movable plate drives the conical ruler to move through the transverse plate, the conical ruler carries out crushing and turning treatment on the material of the material plate, different parts of the material are treated to be in contact with cold air, water condensation in the material is facilitated, meanwhile, the rotating rod drives the cam to rotate, the cam periodically drives the U-shaped plate to move upwards, the U-shaped plate pushes the material plate to shake, the material is further driven to shake, different parts of the material are further promoted to be treated to be in contact;
according to the invention, the flitch is inserted on the U-shaped plate and the C-shaped supporting plate of the mounting and supporting structure, meanwhile, the C-shaped supporting plate is provided with the limiting slide block through the connecting block, the limiting slide block is convenient to slide in the limiting slide groove, the U-shaped plate is convenient to slide up and down, the U-shaped plate is convenient to drive the flitch to move, then, the spring of the fixing structure drives the L-shaped rod to move, the L-shaped rod is inserted at the front end of the C-shaped supporting plate to be inserted, the C-shaped supporting plate is convenient to fix.
The probiotic freeze-dried powder prepared by taking the modified probiotic freeze-dried powder, stachyose, desalted whey powder, whey concentrated protein powder, edible essence, prebiotics, taurine, mixed vitamins and trehalose as raw materials can accelerate the decomposition of food in the small intestine, inhibit the survival and propagation of putrefying bacteria, change the metabolic pathway of protein, eliminate the release of volatile organic gases such as ammonia gas and hydrogen sulfide, and simultaneously, is easy to absorb mineral substances, vitamins and dietary fibers by the intestinal tract.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a preparation method of the present invention;
FIG. 2 is a schematic structural view of the present invention;
FIG. 3 is a right side view of the structure of the present invention;
FIG. 4 is a right top view of the inventive structure;
FIG. 5 is a cross-sectional view of the structure of the present invention;
FIG. 6 is a bottom view in cross-section of the structure of the present invention;
FIG. 7 is a left side view of the fastening structure and its attachment structure of the present invention;
FIG. 8 is a schematic view of the thread groove and the connection structure thereof according to the present invention;
FIG. 9 is a schematic view of a limiting slider and its connecting structure according to the present invention;
FIG. 10 is an enlarged view of the structure at A of FIG. 4 according to the present invention; (ii) a
FIG. 11 is an enlarged view of the structure at B of FIG. 8 according to the present invention;
in the drawings, the components represented by the respective reference numerals are listed below:
1. the air suction pump comprises a bottom plate 2, a door plate 3, a hinge 4, an observation window 5, a controller 6, a box body 7, an accelerating structure 701, a first belt pulley 702, a second belt pulley 703, a driving motor 704, a belt 705, a rotating rod 706, a straight groove 707, a taper rule 708, a transverse plate 709, a sliding plate 710, a threaded groove 711, a cam 712, a spring 713, a movable plate 8, a lock catch 9, a vacuumizing structure 901, a transverse pipe 902, a straight pipe 903, an air outlet pipe 904, an electromagnetic valve 905, an air suction pump 10, a mounting support structure, a limiting sliding groove 1002, a U-shaped plate 1003, a C-shaped support plate 1004, a limiting slide 1005, a connecting block 11, a condensation pipe 12, a material plate 13, a fixing structure 1301, a transverse groove 1302, a spring 1303, a straight block 1304.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further described with reference to the following examples.
Example 1
As shown in fig. 1, a probiotic freeze-dried powder is prepared from a probiotic freeze-dried powder prepared by using vacuum freeze-drying equipment, wherein the probiotic freeze-dried powder comprises the following components in parts by weight: 30-40 parts of modified probiotic freeze-dried powder, 5-10 parts of stachyose, 5-15 parts of desalted whey powder, 5-15 parts of whey protein concentrate powder, 0.5-0.9 part of edible essence, 1-6 parts of prebiotics, 0.01-0.1 part of taurine, 0.5-1 part of mixed vitamins and 2-4 parts of trehalose.
A preparation process of probiotic freeze-dried powder prepared by using vacuum freeze-drying equipment for preparing probiotic freeze-dried powder comprises the following steps:
respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing for 32-38h at 35-40 ℃, inoculating a test tube strain into a triangular flask liquid MRS culture medium, fermenting and culturing for 36-40h at 42-45 ℃;
step two, preparing a fermentation culture medium by taking modified probiotic freeze-dried powder, stachyose, desalted whey powder, whey protein concentrate powder, edible essence, prebiotics, taurine, mixed vitamins and trehalose as raw materials, sterilizing the fermentation culture medium, inoculating a strain expanding solution according to the inoculation amount of 3-5% v/v, carrying out sealed culture at 30-37 ℃ for 1-2 days, stopping fermentation when the pH is reduced to 3.5-4.0, and carrying out anaerobic fermentation at the fermentation temperature of 35-40 ℃;
thirdly, centrifuging the fermented probiotic fermentation liquor at a high speed at the revolution of 8000-;
and step four, adding the obtained oral cavity probiotic bacteria mud into a protective agent to embed probiotic bacteria, and putting the embedded probiotic bacteria into a freeze-drying machine to obtain freeze-dried powder after freeze drying. Acid ester, talcum powder, fluorosilicone rubber, antioxidant and ultraviolet absorbent;
the probiotic freeze-dried powder prepared by taking the modified probiotic freeze-dried powder, stachyose, desalted whey powder, whey protein concentrate powder, edible essence, prebiotics, taurine, mixed vitamins and trehalose as raw materials can accelerate the decomposition of food in the small intestine, inhibit the survival and reproduction of putrefying bacteria, change the metabolic pathway of protein, eliminate the release of volatile organic gases such as ammonia gas and hydrogen sulfide, and simultaneously, is easy for minerals, vitamins and dietary fibers absorbed by the intestinal tract.
Example 2
Example 2 is a further modification to example 1.
Based on the above process, as shown in fig. 2, 3, 4, 5, 6, 8, 9, 10, and 11, a vacuum freeze-drying apparatus for preparing probiotic freeze-dried powder comprises a bottom plate 1, a door panel 2, a hinge 3, an observation window 4, a controller 5, a box 6, a lock catch 8, and a material plate 12, wherein the top of the bottom plate 1 is fixedly connected with the box 6, an accelerating structure 7, and a vacuumizing structure 9, the hinge 3 is uniformly and fixedly connected to the front end of the left side wall of the box 6, the door panel 2 is fixedly connected to the hinge 3, the observation window 4 for observation is fixedly connected in an observation hole of the front side wall of the door panel 2, the controller 5 is fixedly connected to the front side wall of the door panel 2, the lock catch 8 is fixedly connected to the door panel 2, and the door;
the vacuumizing structure 9 comprises a transverse pipe 901, a straight pipe 902, an air outlet pipe 903, an electromagnetic valve 904 and an air pump 905, wherein the air outlet pipe 903 is fixedly connected to the output end of the air pump 905, the straight pipe 902 is fixedly connected to the input end of the air pump 905, the electromagnetic valve 904 is fixedly connected to the bottom of the straight pipe 902, the straight pipe 902 is uniformly and fixedly connected with the transverse pipe 901 for air extraction, and the electromagnetic valve 904 is fixedly installed on the bottom plate 1 through a fixing bolt;
the accelerating structure 7 comprises a first belt pulley 701, a second belt pulley 702, a driving motor 703, a belt 704, a rotating rod 705, a straight groove 706, a taper rule 707, a horizontal plate 708, a sliding plate 709, a threaded groove 710, a cam 711, a spring 712 and a movable plate 713, wherein the output end of the driving motor 703 is fixedly connected with the second belt pulley 702, the second belt pulley 702 is uniformly and movably connected with the first belt pulley 701, the first belt pulley 701 is fixedly connected with the rotating rod 705, the threaded groove 710 is arranged at the position of the rotating rod 705 in the box body 6, the cams 711 are respectively and fixedly connected with the rotating rod 705 at the two ends of the threaded groove 710, the first belt pulley 701 is in threaded connection with the sliding plate 709 through the threaded groove 710, the straight groove 706 is arranged at the inner bottom of the sliding plate 709, the spring 712 is uniformly and fixedly connected with the inner top of the straight groove 706, the movable plate 713 is fixedly connected with the bottom, the bottom of the movable plate 713 is fixedly connected with a transverse plate 708, the bottom of the transverse plate 708 is uniformly and fixedly connected with tapered rulers 707, the tapered rulers 707 are in fit sliding connection with the inner top of the material plate 12, the side wall of the box body 6 is rotationally connected with the rotating rod 705 through a bearing fixedly connected with the side wall, the transverse pipes 901 and the rotating rods 705 are installed in a one-to-one correspondence manner, the rear end of the transverse plate 708 is in fit sliding connection with the inner wall of the material plate 12, the driving motor 703 is fixedly connected with the bottom plate 1 through a mounting frame fixedly connected with the driving motor 703, the driving motor 703 of the accelerating structure 7 drives the second belt pulley 702 to rotate, the second belt pulley 702 drives the belt 704 to move, the belt 704 drives the first belt pulley 701 to rotate, the first belt pulley 701 drives the rotating rod 705 to rotate, the rotating rod 705 drives the sliding plate 709 to, the taper ruler 707 is used for crushing and turning over materials of the material plate 12, different parts of the materials are processed to be in contact with cold air, water in the materials is favorably condensed, meanwhile, the rotating rod 705 drives the cam 711 to rotate, the cam 711 periodically drives the U-shaped plate 1002 to move upwards, the U-shaped plate 1002 pushes the material plate 12 to shake, the materials are further driven to shake, different parts of the materials are further promoted to be processed to be in contact with the cold air, and the water in the materials is favorably condensed;
a condensing pipe 11 for refrigeration is fixedly and uniformly connected to the box body 6 below the first belt pulley 701, and a temperature sensor 14 for temperature detection is fixedly connected to the inner wall of the box body 6;
the box body 6 is connected with a mounting and supporting structure 10 for mounting and supporting the flitch 12, the mounting and supporting structure 10 comprises a limiting sliding groove 1001, a U-shaped plate 1002, a C-shaped supporting plate 1003, a limiting sliding block 1004 and a connecting block 1005, the limiting sliding groove 1001 is arranged on the rear side wall of the box body 6, the limiting sliding groove 1001 is connected with the limiting sliding block 1004 in a fitting and sliding manner, the front side wall of the limiting sliding block 1004 is fixedly connected with the connecting block 1005 at the position corresponding to the rotating rod 705, the bottom of the connecting block 1005 is fixedly connected with the U-shaped plate 1002, the top of the flitch 12 is connected with the top of the U-shaped plate 1002 in a fitting and sliding manner, the bottom of the U-shaped plate 1002 is fixedly connected with the C-shaped supporting plate 1003, the top of the C-shaped supporting plate 1003 is connected with the bottom of the flitch 12 in a fitting and sliding manner, the limiting sliding block, meanwhile, the limiting slide block 1004 is mounted on the C-shaped supporting plate 1003 through the connecting block 1005, the limiting slide block 1004 is convenient to slide in the limiting slide groove 1001, the U-shaped plate 1002 can slide up and down conveniently, and the U-shaped plate 1002 can drive the flitch 12 to move conveniently.
Example 3
Example 3 is a further modification to example 1.
As shown in fig. 7, the flitch 12 is connected with a fixing structure 13 for fixedly connecting the flitch 12 with the U-shaped plate 1002, the fixing structure 13 comprises a transverse slot 1301, a spring 1302, straight piece 1303 and L shape pole 1304, the top front end at flitch 12 is seted up to the cross slot 1301, laminating sliding connection has L shape pole 1304 in the cross slot 1301, flitch 12 is at the straight piece 1303 of the straight piece of the bottom outer end fixedly connected with of cross slot 1301, the inner wall fixedly connected with spring 1302 of straight piece 1303, the inner end of spring 1302 and the upright position fixed connection of L shape pole 1304, the horizontal position of L shape pole 1304 is pegged graft with the front end jack of C shape backup pad 1003, fixed knot constructs 13 symmetry and sets up the front end of flitch 12, fixed knot constructs 13's spring 1302 drives L shape pole 1304 and removes, L shape pole 1304 is inserted in the front end of C shape backup pad 1003 and is pegged graft, make things convenient for C shape backup pad 1003 to fix spacingly to flitch 12, flitch 12 fixed mounting has been guaranteed in U.
When the device is used, the flitch 12 is inserted on the U-shaped plate 1002 and the C-shaped support plate 1003 of the mounting and supporting structure 10, the C-shaped support plate 1003 is provided with the limit slider 1004 through the connecting block 1005, the limit slider 1004 is convenient to slide in the limit sliding groove 1001, the U-shaped plate 1002 is convenient to slide up and down, the U-shaped plate 1002 is convenient to drive the flitch 12 to move, then the spring 1302 of the fixed structure 13 drives the L-shaped rod 1304 to move, the L-shaped rod 1304 is inserted at the front end of the C-shaped support plate 1003 for inserting, the C-shaped support plate 1003 is convenient to fix and limit the flitch 12, the flitch 12 is ensured to be fixedly arranged in the U-shaped plate 1002, the controller 5 starts the driving motor 703, the condenser pipe 11 and the condenser pipe 11 of the accelerating structure 7, the condenser pipe 11 is used for air cooling treatment, the temperature sensor 14 is used for monitoring, the belt 704 drives the first belt pulley 701 to rotate, the first belt pulley 701 drives the rotating rod 705 to rotate, the rotating rod 705 drives the sliding plate 709 to move through the threaded groove 710, the sliding plate 709 drives the movable plate 713 to move, the movable plate 713 drives the taper rule 707 to move through the transverse plate 708, the taper rule 707 performs crushing and turning processing on the material of the material plate 12, different parts of the material are processed to be in contact with cold air, water condensation in the material is facilitated, meanwhile, the rotating rod 705 drives the cam 711 to rotate, the cam 711 periodically drives the U-shaped plate 1002 to move upwards, the U-shaped plate 1002 pushes the material plate 12 to shake, the material shaking is further driven, different parts of the material are further promoted to be processed to be in contact with the cold air, water condensation in the material is further facilitated, then the electromagnetic valve 904 and the air pump 905 of the vacuumizing structure 9 are started, the air pump 905 is, vacuum freeze drying is realized.
In the description herein, reference to the description of the terms "one embodiment," "an example," "a specific example," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (10)
1. A probiotic freeze-dried powder is characterized in that: the probiotic freeze-dried powder comprises the following components in parts by weight: 30-40 parts of modified probiotic freeze-dried powder, 5-10 parts of stachyose, 5-15 parts of desalted whey powder, 5-15 parts of whey protein concentrate powder, 0.5-0.9 part of edible essence, 1-6 parts of prebiotics, 0.01-0.1 part of taurine, 0.5-1 part of mixed vitamins and 2-4 parts of trehalose.
2. The preparation process of the probiotic freeze-dried powder according to claim 1, which is characterized in that: the preparation process comprises the following steps:
respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing for 32-38h at 35-40 ℃, inoculating a test tube strain into a triangular flask liquid MRS culture medium, fermenting and culturing for 36-40h at 42-45 ℃;
step two, preparing a fermentation culture medium by taking modified probiotic freeze-dried powder, stachyose, desalted whey powder, whey protein concentrate powder, edible essence, prebiotics, taurine, mixed vitamins and trehalose as raw materials, inoculating a strain expanding solution according to the inoculation amount of 3-5% (v/v) after sterilizing the fermentation culture medium, carrying out sealed culture at 30-37 ℃ for 1-2 days, stopping fermentation when the pH value is reduced to 3.5-4.0, and carrying out anaerobic fermentation at the fermentation temperature of 35-40 ℃;
thirdly, centrifuging the fermented probiotic fermentation liquor at a high speed at the revolution of 8000-;
and step four, adding the obtained oral cavity probiotic bacteria mud into a protective agent to embed probiotic bacteria, and putting the embedded probiotic bacteria into a freeze-drying machine to obtain freeze-dried powder after freeze drying.
3. The vacuum freeze-drying equipment for preparing probiotic freeze-dried powder according to claim 1, comprising a bottom plate (1), a door plate (2), a hinge (3), an observation window (4), a controller (5), a box body (6), a lock catch (8) and a material plate (12), and is characterized in that: the top of the bottom plate (1) is fixedly connected with a box body (6), an accelerating structure (7) and a vacuumizing structure (9), the front end of the left side wall of the box body (6) is uniformly and fixedly connected with a hinge (3), the hinge (3) is fixedly connected with a door panel (2), an observation window (4) for observation is fixedly connected in an observation hole of the front side wall of the door panel (2), the front side wall of the door panel (2) is fixedly connected with a controller (5), the door panel (2) is fixedly connected with a lock catch (8), and the door panel (2) is fixedly connected with the box body (6) through the lock catch (8);
the vacuumizing structure (9) comprises a transverse pipe (901), a straight pipe (902), an air outlet pipe (903), an electromagnetic valve (904) and an air pump (905), wherein the air outlet pipe (903) is fixedly connected to the output end of the air pump (905), the straight pipe (902) is fixedly connected to the input end of the air pump (905), the electromagnetic valve (904) is fixedly connected to the bottom of the straight pipe (902), and the transverse pipe (901) used for air extraction is uniformly and fixedly connected to the straight pipe (902);
the accelerating structure (7) comprises a first belt pulley (701), a second belt pulley (702), a driving motor (703), a belt (704), a rotating rod (705), a straight groove (706), a taper ruler (707), a transverse plate (708), a sliding plate (709), a threaded groove (710), a cam (711), a spring (712) and a movable plate (713), the output end of the driving motor (703) is fixedly connected with the second belt pulley (702), the second belt pulley (702) is uniformly and movably connected with the first belt pulley (701), the first belt pulley (701) is fixedly connected with the rotating rod (705), the threaded groove (710) is formed in the rotating rod (705) at the position in the box body (6), the cams (711) are respectively and fixedly connected to the two ends of the threaded groove (710) through the threaded groove (710), the sliding plate (709) is in threaded connection with the first belt pulley (701), a straight groove (706) is formed in the inner bottom of the sliding plate (709), a spring (712) is uniformly and fixedly connected to the inner top of the straight groove (706), a movable plate (713) is fixedly connected to the bottom of the spring (712), the outer wall of the movable plate (713) is in fit sliding connection with the inner wall of the straight groove (706), a transverse plate (708) is fixedly connected to the bottom of the movable plate (713), a taper ruler (707) is uniformly and fixedly connected to the bottom of the transverse plate (708), and the taper ruler (707) is in fit sliding connection with the inner top of the flitch (12);
a condensing pipe (11) for refrigeration is fixedly and uniformly connected to the box body (6) below the first belt pulley (701), and a temperature sensor (14) for temperature detection is fixedly connected to the inner wall of the box body (6);
the box body (6) is connected with an installation supporting structure (10) for installing and supporting the material plate (12), the mounting and supporting structure (10) comprises a limiting sliding groove (1001), a U-shaped plate (1002), a C-shaped supporting plate (1003), a limiting sliding block (1004) and a connecting block (1005), the limiting sliding groove (1001) is arranged on the rear side wall of the box body (6), a limiting sliding block (1004) is connected in the limiting sliding groove (1001) in a fitting and sliding manner, the front side wall of the limiting slide block (1004) is fixedly connected with a connecting block (1005) at the position corresponding to the rotating rod (705), the bottom of the connecting block (1005) is fixedly connected with a U-shaped plate (1002), the top of the flitch (12) is jointed and glidingly connected with the top of the U-shaped plate (1002), the bottom of the U-shaped plate (1002) is fixedly connected with a C-shaped supporting plate (1003), the top of the C-shaped supporting plate (1003) is attached to the bottom of the material plate (12) in a sliding connection mode;
flitch (12) are connected with fixed knot who is used for flitch (12) and U-shaped board (1002) fixed connection and construct (13), fixed knot constructs (13) including horizontal slot (1301), spring (1302), straight piece (1303) and L shape pole (1304), the top front end at flitch (12) is seted up in horizontal slot (1301), laminating sliding connection has L shape pole (1304) in horizontal slot (1301), flitch (12) are at the bottom outer end fixedly connected with straight piece (1303) of horizontal slot (1301), the inner wall fixedly connected with spring (1302) of straight piece (1303), the inner end of spring (1302) and the position fixed connection that stands vertically of L shape pole (1304), the horizontal position of L shape pole (1304) is pegged graft with the front end jack of C shape backup pad (1003).
4. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 3, characterized in that: the electromagnetic valve (904) is fixedly installed on the bottom plate (1) through a fixing bolt.
5. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 3, characterized in that: the lateral wall of box (6) is connected with dwang (705) through fixed connection's bearing and rotation, and violently pipe (901) and dwang (705)'s installation is one-to-one.
6. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 5, characterized in that: the rear end of the transverse plate (708) is attached to the inner wall of the material plate (12) in a sliding connection mode.
7. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 6, characterized in that: the driving motor (703) is fixedly connected with the bottom plate (1) through a mounting frame fixedly connected with the driving motor.
8. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 3, characterized in that: the limiting sliding block (1004) is in a dovetail shape, an L shape or a T shape.
9. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 8, characterized in that: the C-shaped supporting plate (1003) is connected with the cam (711) in a sliding mode.
10. The vacuum freeze-drying equipment for preparing the probiotic freeze-dried powder according to claim 3, characterized in that: the fixed structure (13) is symmetrically provided with the front ends of the material plates (12).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117268048A (en) * | 2022-07-12 | 2023-12-22 | 杭州诺莘科技有限责任公司 | Efficient freeze-drying device for producing probiotics freeze-dried powder |
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Application publication date: 20210511 |