CN111334448A - Preparation process of oral probiotic freeze-dried powder - Google Patents
Preparation process of oral probiotic freeze-dried powder Download PDFInfo
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- CN111334448A CN111334448A CN201811552093.3A CN201811552093A CN111334448A CN 111334448 A CN111334448 A CN 111334448A CN 201811552093 A CN201811552093 A CN 201811552093A CN 111334448 A CN111334448 A CN 111334448A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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Abstract
A preparation process of oral probiotic freeze-dried powder relates to a microbial freeze-drying technology, in particular to a preparation process of oral probiotic freeze-dried powder. Provides a preparation process of oral probiotic freeze-dried powder which is suitable for production operation and has ideal effect. Preparing a seed solution: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in MRS test tube culture medium, culturing at 35-40 deg.C for 24-28h, inoculating test tube strain into triangular flask liquid MRS culture medium, fermenting and culturing at 35-40 deg.C for 36-40 h; fermentation: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, wherein the fermentation temperature is 35-40 ℃, and performing anaerobic fermentation to obtain oral probiotic fermentation liquor; centrifuging: centrifuging the fermented fermentation liquid at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic bacterial sludge; embedding: adding the obtained bacterial sludge into a protective agent to embed probiotic strains; and (3) freeze drying: and (3) placing the embedded probiotics in a freeze drying machine, and freeze-drying to prepare freeze-dried powder. The preparation process is simple and is suitable for production operation.
Description
Technical Field
The invention relates to a microbial freeze-drying technology, in particular to a preparation process of oral probiotic freeze-dried powder.
Background
Probiotics refer to certain microbial agents or fermented biologics that produce beneficial physiological effects on the host by modulating host immune system function and improving host flora balance. Oral probiotics, which are a kind of oral bacteria, are considered to have a function of improving the micro-ecological environment of the oral cavity of a human body. The oral cavity probiotics have the functions of improving the micro-ecological environment of the oral cavity, eliminating oral cavity peculiar smell, inhibiting the endotoxin production of the oral cavity pathogenic bacteria and the like. The research and development of the oral probiotics can bring positive significance to oral treatment and daily care.
However, the oral cavity probiotics in the prior art are easy to inactivate before being produced, prepared, stored and entered into the oral cavity, and probiotics with ideal effects cannot be obtained.
Disclosure of Invention
Aiming at the problems, the invention provides a preparation process of oral probiotic freeze-dried powder which is suitable for production operation and has ideal effect.
The technical scheme of the invention is as follows: the method comprises the following steps:
1) and preparing seed liquid: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in MRS test tube culture medium, culturing at 35-40 deg.C for 24-28h, inoculating test tube strain into triangular flask liquid MRS culture medium, fermenting and culturing at 35-40 deg.C for 36-40 h;
2) and fermenting: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, wherein the fermentation temperature is 35-40 ℃, and performing anaerobic fermentation to obtain oral probiotic fermentation liquor;
3) and centrifuging: centrifuging the fermented oral probiotic fermentation liquor at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic mud;
4) and embedding: adding the obtained oral cavity probiotic bacteria mud into a protective agent to embed probiotic bacteria;
5) and (3) freeze drying: and (3) placing the embedded probiotics in a freeze drying machine, and freeze-drying to prepare freeze-dried powder.
The protective agent in the step 4) comprises 85-95 parts of skim milk powder, 2-4 parts of trehalose, 4-6 parts of xylitol and 1-3 parts of inulin by mass.
The protective agent and the bacterial sludge in the step 4) are mixed according to the weight ratio of 1: 20 an embedding operation.
The freeze drying conditions in the step 5) are as follows: the minimum temperature is-40 ℃, the freezing time is 6-8h, and the total drying time is 36-40 h.
The invention has the beneficial effects that: protecting the probiotics by adopting an embedding technology to prevent the probiotics from being inactivated so as to obtain high-quality oral probiotics; and then freeze-drying, wherein the freeze-drying further protects the probiotics to ensure that the quality of the probiotics reaches the highest and the effect reaches the best. The preparation process is simple and is suitable for production operation.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
The preparation process of the oral probiotic freeze-dried powder comprises the following steps:
1) and preparing seed liquid: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing at 35 ℃ for 24h, inoculating a test tube strain into a triangular flask liquid MRS culture medium, fermenting and culturing at 35 ℃ for 36 h;
2) and fermenting: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, and performing anaerobic fermentation at the fermentation temperature of 35 ℃ to obtain oral probiotic fermentation liquor;
3) and centrifuging: centrifuging the fermented oral probiotic fermentation liquor at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic mud;
4) and embedding: adding the obtained oral cavity probiotic bacterial mud into a protective agent to embed probiotic bacterial, wherein the protective agent and the bacterial mud are mixed according to the weight ratio of 1: 20, embedding operation; the protective agent comprises 85 parts of skim milk powder, 2 parts of trehalose, 4 parts of xylitol and 1 part of inulin by mass;
5) and (3) freeze drying: and (2) placing the embedded probiotics into a freeze drying machine, and freeze-drying to prepare freeze-dried powder, wherein the freeze-drying conditions are as follows: the minimum temperature is-40 ℃, the freezing time is 6h, and the total drying time is 36 h.
Example 2
The preparation process of the oral probiotic freeze-dried powder comprises the following steps:
1) and preparing seed liquid: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing for 28h at 40 ℃, then inoculating test tube strains into a triangular flask liquid MRS culture medium for fermentation culture, and culturing for 340h at 40 ℃;
2) and fermenting: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, fermenting at 40 ℃, and performing anaerobic fermentation to obtain oral probiotic fermentation liquor;
3) and centrifuging: centrifuging the fermented oral probiotic fermentation liquor at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic mud;
4) and embedding: adding the obtained oral cavity probiotic bacterial mud into a protective agent to embed probiotic bacterial, wherein the protective agent and the bacterial mud are mixed according to the weight ratio of 1: 20 an embedding operation. (ii) a The protective agent comprises 95 parts of skim milk powder, 4 parts of trehalose, 6 parts of xylitol and 3 parts of inulin by mass;
5) and (3) freeze drying: and (2) placing the embedded probiotics into a freeze drying machine, and freeze-drying to prepare freeze-dried powder, wherein the freeze-drying conditions are as follows: the minimum temperature is-40 ℃, the freezing time is 8 hours, and the total drying time is 40 hours.
Example 3
The preparation process of the oral probiotic freeze-dried powder comprises the following steps:
1) and preparing seed liquid: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in an MRS test tube culture medium, culturing at 37 ℃ for 26h, inoculating a test tube strain into a triangular flask liquid MRS culture medium, fermenting and culturing at 37 ℃ for 38 h;
2) and fermenting: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, wherein the fermentation temperature is 35-40 ℃, and performing anaerobic fermentation to obtain oral probiotic fermentation liquor;
3) and centrifuging: centrifuging the fermented oral probiotic fermentation liquor at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic mud;
4) and embedding: adding the obtained oral cavity probiotic bacterial mud into a protective agent to embed probiotic bacterial, wherein the protective agent and the bacterial mud are mixed according to the weight ratio of 1: 20 an embedding operation. (ii) a The protective agent comprises 90 parts of skim milk powder, 3 parts of trehalose, 5 parts of xylitol and 2 parts of inulin by mass;
5) and (3) freeze drying: and (2) placing the embedded probiotics into a freeze drying machine, and freeze-drying to prepare freeze-dried powder, wherein the freeze-drying conditions are as follows: the minimum temperature is-40 ℃, the freezing time is 7h, and the total drying time is 38 h.
Claims (4)
1. A preparation process of oral probiotic freeze-dried powder is characterized by comprising the following steps:
1) and preparing seed liquid: respectively inoculating lactobacillus salivarius, lactobacillus plantarum and lactobacillus paracasei in MRS test tube culture medium, culturing at 35-40 deg.C for 24-28h, inoculating test tube strain into triangular flask liquid MRS culture medium, fermenting and culturing at 35-40 deg.C for 36-40 h;
2) and fermenting: inoculating each seed solution into a fermentation tank according to the inoculation amount of 3%, wherein the fermentation temperature is 35-40 ℃, and performing anaerobic fermentation to obtain oral probiotic fermentation liquor;
3) and centrifuging: centrifuging the fermented oral probiotic fermentation liquor at a high speed of 10000rpm/min by a tubular centrifuge to obtain probiotic mud;
4) and embedding: adding the obtained oral cavity probiotic bacteria mud into a protective agent to embed probiotic bacteria;
5) and (3) freeze drying: and (3) placing the embedded probiotics in a freeze drying machine, and freeze-drying to prepare freeze-dried powder.
2. The preparation process of the oral probiotic freeze-dried powder according to claim 1, wherein the protective agent in the step 4) comprises 85-95 parts of skim milk powder, 2-4 parts of trehalose, 4-6 parts of xylitol and 1-3 parts of inulin by mass.
3. The preparation process of the oral probiotic freeze-dried powder according to claim 1, wherein the protective agent and the bacterial sludge in the step 4) are mixed according to a weight ratio of 1: 20 an embedding operation.
4. The process for preparing an oral probiotic freeze-dried powder according to claim 1, wherein the freeze-drying conditions in the step 5) are as follows: the minimum temperature is-40 ℃, the freezing time is 6-8h, and the total drying time is 36-40 h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811552093.3A CN111334448A (en) | 2018-12-19 | 2018-12-19 | Preparation process of oral probiotic freeze-dried powder |
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| CN201811552093.3A CN111334448A (en) | 2018-12-19 | 2018-12-19 | Preparation process of oral probiotic freeze-dried powder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772927A (en) * | 2020-12-31 | 2021-05-11 | 重庆第二师范学院 | Probiotic freeze-dried powder |
| CN114917176A (en) * | 2022-03-14 | 2022-08-19 | 江南大学 | Compound probiotics capable of improving ozostomia |
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2018
- 2018-12-19 CN CN201811552093.3A patent/CN111334448A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772927A (en) * | 2020-12-31 | 2021-05-11 | 重庆第二师范学院 | Probiotic freeze-dried powder |
| CN114917176A (en) * | 2022-03-14 | 2022-08-19 | 江南大学 | Compound probiotics capable of improving ozostomia |
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