CN112754988A - Probiotic skin care product for reducing skin sensitization and preparation method thereof - Google Patents
Probiotic skin care product for reducing skin sensitization and preparation method thereof Download PDFInfo
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- CN112754988A CN112754988A CN202110300709.3A CN202110300709A CN112754988A CN 112754988 A CN112754988 A CN 112754988A CN 202110300709 A CN202110300709 A CN 202110300709A CN 112754988 A CN112754988 A CN 112754988A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention discloses a probiotic skin care product for reducing skin sensitization and a preparation method thereof, wherein the probiotic skin care product takes active probiotic freeze-dried powder as an active raw material, probiotics in the active probiotic freeze-dried powder are lactobacillus rhamnosus and lactobacillus delbrueckii, and the preferred quantity ratio of the lactobacillus rhamnosus to the lactobacillus delbrueckii is 0.5:1-2: 1. The probiotic skin care product can be in the form of an active moisturizing spray, and preferably comprises the following components in percentage by mass: 2-10% of active probiotic freeze-dried powder, 2000IU/mL of recombinant human epidermal growth factor and the balance of physiological saline. The preparation method is simple and easy to popularize, can be used for moisturizing and maintaining the skin of the whole body including facial care, can effectively inhibit skin allergy or inflammatory reaction, reduces skin allergenicity, enhances the resistance of the skin to bacterial infection, and promotes skin barrier repair.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a probiotic skin care product for reducing skin sensitization and a preparation method thereof.
Background
The skin care products are skin care cosmetics with the functions of beautifying and face, and have various types and different characteristics according to different specific efficacies and application modes. The skin care products can be mainly divided into the following from the specific function and effect: cleaning, moistening, whitening, and antiaging. Among them, skin-moisturizing products are required to improve the moisturizing effect on the skin surface, thereby maintaining skin moisturization. The moisture-retaining skin care products on the market mainly include other types such as liquid, gel and facial mask in appearance.
With the rapid development and progress of the cosmetic industry, new types of skin care products are continuously appeared on the market, wherein skin care products containing probiotic components are introduced by many brand manufacturers. Probiotics are microorganisms beneficial to human and animal bodies and are widely applied to the fields of bioengineering, food health care and life health. It is reported that skin care products containing probiotics can play a role in inhibiting harmful bacteria, preserving water, moisturizing and the like when being smeared on skin.
However, most of the probiotic skin care products on the market do not consider the biological activity of probiotics, and products with the functions of activity, allergy resistance and the like are few, so that the consistency of the product effect with time is difficult to determine.
Disclosure of Invention
In view of the above, the present application provides a probiotic skin care product capable of reducing skin sensitization and a preparation method thereof, and the probiotic skin care product provided by the present application can inhibit skin allergy or inflammatory reaction, reduce skin sensitization, and ensure consistency and effectiveness of an active formula in a preparation-and-use mode.
The invention provides application of active probiotic freeze-dried powder in preparation of a probiotic skin care product for reducing skin sensitization, wherein probiotics in the active probiotic freeze-dried powder are lactobacillus rhamnosus and lactobacillus delbrueckii.
The anti-sensitization mechanism of the probiotic skin care product is as follows: the active probiotic freeze-dried powder contains active lactobacillus rhamnosus and lactobacillus delbrueckii which have synergistic effect on the surface of the skin, so that the allergenicity is effectively reduced, the skin immunity is adjusted, and the resistance of the skin to pathogenic bacteria such as staphylococcus aureus is laterally enhanced.
In some embodiments of the present application, the immunogenicity of the lactobacillus rhamnosus and lactobacillus delbrueckii post-formulation comprises one or more of:
reducing the expression of proinflammatory cytokine IL-6;
increasing the expression of the cytokine IL-10 with anti-inflammatory action;
the induction capacity to the immune tolerance dendritic cells is improved;
inducing M2 type macrophage with immunoregulation function.
The embodiment of the invention provides a probiotic skin care product for reducing skin sensitization, which takes active probiotic freeze-dried powder as an active raw material, wherein probiotics in the active probiotic freeze-dried powder are lactobacillus rhamnosus and lactobacillus delbrueckii, and the number ratio of the lactobacillus rhamnosus to the lactobacillus delbrueckii is 0.5:1-2: 1.
Further preferably, the number ratio of lactobacillus rhamnosus to lactobacillus delbrueckii is 1: 1.
Lactobacillus rhamnosus (l.rhamnosus) belongs to the genus lactobacillus and is one of the normal flora of the human body. The lactobacillus delbrueckii is an important strain of lactobacillus, and has wide application prospect in the industries of food, medicine and health care, chemical industry and the like. In the examples of the present application, the species of probiotic bacteria are as follows: lactobacillus rhamnosus ATCC 9595; lactobacillus delbrueckii PTCC: 1743; DSM 20072.
The active probiotic powder related to the embodiment of the application can be directly purchased and obtained, and the lactobacillus rhamnosus freeze-dried powder and the lactobacillus delbrueckii freeze-dried powder which are sold in the market are mixed to obtain the active probiotic powder. In the experimental process, the commercial brand freeze-dried powder is purchased respectively and verified according to the same proportion, and no obvious influence is found. Therefore, the preparation can be selected as long as the activity of the lactobacillus rhamnosus and the lactobacillus delbrueckii in the freeze-dried powder meets the requirement. The application has no special requirements for specific subspecies of probiotics and the like, and the active raw material has certain biological activity, for example, the number of single viable bacteria is about 2 multiplied by 106CFU/mL. Book (I)The probiotic species of the lactobacillus rhamnosus and the lactobacillus delbrueckii selected by the invention and the preferred proportion thereof have the advantages of wider applicability to human groups and lower allergenicity.
Some embodiments of the present application detect the immunogenicity of the active materials obtained by cell experiments; the invention adopts the active probiotic freeze-dried powder containing the lactobacillus rhamnosus and the lactobacillus delbrueckii, and the two probiotics with activity are compounded, so that the skin allergy or inflammatory reaction can be better inhibited, the skin sensitization is reduced, the skin immunity is adjusted, and the antibacterial ability is enhanced compared with a single preparation.
In the invention, the active probiotic freeze-dried powder can be directly applied to skin, is preferably dissolved in water to prepare a skin care product solution, and is more preferably prepared at any time, so that the consistency and the effectiveness of an active formula are effectively ensured. In a preferred embodiment of the application, the probiotic skin care product consists of the following raw materials in percentage by mass: 2-10% of active probiotic freeze-dried powder and the balance of pure water or normal saline; the water content can be used for keeping moisture and regulating oil-water balance of skin.
Wherein the pure water is distilled water, pure water and other water which meet the safety requirements of the field. The physiological saline is also called sterile physiological saline, and refers to a sodium chloride aqueous solution which is commonly used in physiological experiments or clinical practice and has osmotic pressure basically equal to that of animal or human plasma; the mass concentration is 0.85-0.9% when used in human body, and the normal form of cells can be maintained when externally used. The dosage of the active probiotic freeze-dried powder can be 3-9%, 5-8% and the like.
Preferably, the probiotic skin care product according to the embodiment of the present application further comprises: human epidermal growth factor with the concentration of more than 1000IU/mL, and preferably recombinant human epidermal growth factor; the amount may be 1500-2000IU/mL, preferably 2000 IU/mL.
Human epidermal growth factor (hEGF) is a small molecular polypeptide consisting of 53 amino acids, has a molecular weight of about 6000 daltons, an isoelectric point of about 4.6, and three pairs of disulfide bonds in a molecule, and is stable against physicochemical factors such as acid, alkali, heat and the like. hEGF has a wide range of biological effects, can strongly promote the growth of various epidermal tissues, and has been used medically for the treatment of ulcers and various wounds. The natural source of hEGF is mainly extracted by tissue and body fluids, which is costly. One approach to scale production of hEGF is by solid phase chemical synthesis, and the other is by genetic engineering techniques to produce recombinant hEGF. The embodiment of the application adopts the commercially available recombinant human epidermal growth factor, and the cost is relatively low.
The application preferably mixes a certain amount of recombinant human epidermal growth factor with the active raw materials, so that the obtained probiotic skin care product has the effects of reducing skin sensitization, enhancing antibacterial capacity and skin barrier repair capacity, wherein the recombinant human epidermal growth factor mainly has a good effect of promoting skin barrier repair. For example, through parallel comparison tests, the repair effect of the recombinant human epidermal growth factor on the skin is obviously better than that of hyaluronic acid under the formula system, and the obvious improvement or inhibition effect is not found in a verification experiment of a control group added with hyaluronic acid.
The embodiment of the invention also provides a preparation method of the probiotic skin care product, which comprises the following steps:
fully mixing the active probiotic freeze-dried powder containing the lactobacillus rhamnosus and the lactobacillus delbrueckii with normal saline, then optionally adding the recombinant human epidermal growth factor into the obtained mixed solution, and shaking up to prepare the liquid probiotic skin care product.
The prepared skin care product containing the active ingredients can be kept uniform and stable within 24 hours at normal temperature or within 30 days of refrigeration at 4-8 ℃, and has the best anti-sensitization effect.
The application has no special limitation on the application mode of the prepared probiotic skin care product, and the liquid probiotic skin care product can be filled into a spray bottle to be prepared into moisturizing spray; or combined with facial mask carrier (such as facial mask paper, non-woven fabric, etc.) to make into moisture keeping facial mask product.
The moisturizing spray is the most common skin-care spray, can moisturize the skin anytime and anywhere, can adjust the oil-water balance of the skin, relieves the skin pressure, and can have certain effects of sun protection, mosquito repelling and the like through control of different components. Compared with other skin care products such as facial masks and the like, the moisturizing spray has the advantages of being more convenient to use, capable of being operated at any time and any place, not limited to facial skin care, and capable of moisturizing and caring whole body skin.
Therefore, the embodiment of the invention aims at the field of skin care products, and preferably prepares the active probiotic moisturizing spray with the functions of reducing skin sensitization, enhancing antibacterial capacity and skin barrier repair capacity; the active moisturizing spray comprises the following components in parts by mass: 2-10% of active probiotic freeze-dried powder, 2000IU/mL of recombinant human epidermal growth factor and the balance of physiological saline.
Specifically, the following technical scheme can be adopted to implement:
fully and uniformly mixing lactobacillus rhamnosus mixed lactobacillus delbrueckii freeze-dried powder (the optimal quantity ratio is 1: 1) and normal saline, then adding 2000IU/mL of recombinant human epidermal growth factor into the obtained mixed solution, and uniformly shaking to prepare active moisturizing spray solution; the prepared active moisturizing spray can be used by being filled into a spray bottle, and the active ingredients of the spray can be uniformly and stably kept within 24 hours at normal temperature or within 30 days of refrigeration at 4-8 ℃, and the anti-sensitization effect is optimal.
The preparation method provided by the embodiment of the invention is simple and easy to popularize, and the obtained product can be used for moisturizing and maintaining the skin of the whole body including facial care, can effectively inhibit skin allergy or inflammatory reaction, reduces skin allergenicity, enhances the resistance of the skin to bacterial infection, and promotes skin barrier repair. In addition, the probiotic skin care product considers the biological activity of the probiotics, and can effectively ensure the consistency and effectiveness of the active formula along with time by a mode of preparation and use at any time.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
For further understanding of the present application, the probiotic skin care product for reducing skin sensitization and the preparation method thereof provided by the present application are specifically described below with reference to the examples.
The following raw material reagents were commercially available.
Example 1
1) Active formula and operation preparation:
will be 4X 107CFU Lactobacillus rhamnosus lyophilized powder and 4 × 107And (3) fully mixing the CFU lactobacillus delbrueckii freeze-dried powder with 20mL of physiological saline to obtain the compound.
2) Cell assay for dendritic cells and macrophages inducing immune tolerance
5 cases of atopic dermatitis patients were isolated from CD14(+) monocytes, and Interleukin (IL) -4 and granulocyte colony stimulating factor (GM-CSF) were added to induce differentiation of monocytes into dendritic cells, and Interferon (IFN) r and GM-CSF were added to induce differentiation of monocytes into macrophages, respectively.
On the 5 th day of in vitro cell culture, induced Dendritic Cells (DC) and macrophages (M.phi.) were divided into four groups, and LPS (LPS group), Lactobacillus rhamnosus (RAM group), Lactobacillus delbrueckii (DEL group), a mixture of Lactobacillus rhamnosus and Lactobacillus delbrueckii (MIX group, obtained as prepared above) was added, and after 2 days of culture, the expression levels of the four groups of cell surface antigens CD80, CD86, HLA-DR and CD163 were detected by flow cytometry, and the expression levels of cytokines IL-6 and IL-10 were detected by ELISA.
The experimental results are as follows:
TABLE 1 Experimental results for dendritic cells inducing immune tolerance
Differences between groups were statistically significant (P < 0.05).
TABLE 2 results of macrophage induction experiments
Differences between groups were statistically significant (P < 0.05).
From the above table results, it can be seen that a mixture of lactobacillus rhamnosus, lactobacillus delbrueckii, lactobacillus rhamnosus and lactobacillus delbrueckii, all of which can reduce the expression of antigen presenting molecules and co-stimulatory molecules of dendritic cells, inducing immune tolerant dendritic cells; wherein the capacity of the mixture of lactobacillus rhamnosus and lactobacillus delbrueckii to induce immune tolerant dendritic cells is better than that of lactobacillus rhamnosus or lactobacillus delbrueckii used alone.
Meanwhile, the mixture of lactobacillus rhamnosus, lactobacillus delbrueckii, lactobacillus rhamnosus and lactobacillus delbrueckii can induce M2 type macrophages with immunoregulation function (the expression of CD80 and CD86 is reduced, and the expression of CD163 is up-regulated), reduce the expression of proinflammatory cytokine IL-6 and increase the expression of cytokine IL-10 with anti-inflammatory action. Moreover, the function of inducing regulatory macrophages by the mixture of lactobacillus rhamnosus and lactobacillus delbrueckii is better than that of the lactobacillus rhamnosus or lactobacillus delbrueckii used alone.
Example 2
Will be 4X 107CFU Lactobacillus rhamnosus lyophilized powder and 4 × 107The freeze-dried powder of the CFU lactobacillus delbrueckii is fully mixed with 20mL of normal saline, 40,000IU of recombinant human epidermal growth factor is added after mixing, 20mL of active moisturizing spray liquid is prepared after shaking up, and the moisturizing spray liquid is poured into a spray bottle for use.
Example 3
Study to 5 normal persons aged 18-35 years, the inner skin of the left forearm of the subject was divided into 3 square areas of 5 × 5cm, and the active moisturizing spray (treatment group a), the lactobacillus rhamnosus and lactobacillus delbrueckii physiological saline solution (positive control group B), and the physiological saline solution (negative control group C) described in example 2 were applied to the inner skin of the left forearm, and the skin was sprayed 3 times a day (in each test skin area, the spray bottle was used to uniformly press and spray the skin 10cm away from the surface of the skin 5 times, the spray bottle was a commercially available press type spray bottle), and after 7 days, the skin was Tested for Epidermal Water Loss (TEWL), stratum corneum water content (WCSC), and skin oil and fat (SC) content.
The experimental results are as follows:
table 3 experimental results of active moisturizing sprays to improve skin physiological function
| Group of | TEWL(g/h/cm2) | WCSC(％) | SC(μg/cm2) |
| Treatment group A | 25.24±2.51 | 29.43±3.12 | 112.34±11.75 |
| Positive control group B | 31.38±1.22 | 22.47±1.77 | 110.54±9.08 |
| Negative control group C | 36.07±1.75 | 18.53±2.16 | 82.35±8.75 |
From the results in the above table, the transepidermal water loss (TEWL) and the stratum corneum water content (WCSC) in the treated group were significantly improved compared to the positive control group and the negative control group, and the differences were statistically significant (P < 0.05). The content of skin oil (SC) in the treatment group is obviously increased compared with that in the negative control group, and the difference has statistical significance, but the content of the skin oil (SC) in the treatment group is not statistically different compared with that in the positive control group. The positive control group has reduced epidermal water loss (TEWL) compared with the negative control group, the Water Content of Stratum Corneum (WCSC) and the content of skin oil (SC) are obviously increased, and the difference has statistical significance. The results show that the treatment composition compound active moisturizing spray can obviously improve the physiological function of the skin and is beneficial to the repair of the barrier function of the skin.
The active formula and the preparation method in the embodiment of the invention are simple and easy to popularize, and the obtained product can be used for moisturizing and caring the skin of the whole body including facial care, can effectively inhibit skin allergy or inflammatory reaction, reduce skin sensitization, enhance the resistance of the skin to bacterial infection and promote skin barrier repair. In addition, the probiotic skin care product takes the biological activity of the probiotics into consideration, and can ensure the consistency and effectiveness of the active formula along with time through a mode of preparation and use at any time.
The foregoing description of the embodiments is provided only to assist and understand the method of the present invention and its core concept, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications also fall into the protection scope of the claims of the present invention.
Claims (9)
1. The application of the active probiotic freeze-dried powder in the preparation of the probiotic skin care product for reducing the skin sensitization is realized, and the probiotics in the active probiotic freeze-dried powder are lactobacillus rhamnosus and lactobacillus delbrueckii.
2. The use of claim 1, wherein the immunogenicity of the lactobacillus rhamnosus and lactobacillus delbrueckii after combination comprises one or more of:
reducing the expression of proinflammatory cytokine IL-6;
increasing the expression of the cytokine IL-10 with anti-inflammatory action;
the induction capacity to the immune tolerance dendritic cells is improved;
inducing M2 type macrophage with immunoregulation function.
3. The probiotic skin care product for reducing skin sensitization is characterized in that active probiotic freeze-dried powder is used as an active raw material, probiotics in the active probiotic freeze-dried powder are lactobacillus rhamnosus and lactobacillus delbrueckii, and the number ratio of the lactobacillus rhamnosus to the lactobacillus delbrueckii is 0.5:1-2: 1.
4. The probiotic skin care product according to claim 3, characterized in that the ratio of the number of Lactobacillus rhamnosus and Lactobacillus delbrueckii is 1: 1.
5. The probiotic skin care product according to claim 3, characterized in that it comprises: 2-10 wt% of active probiotic freeze-dried powder and the balance of pure water or normal saline.
6. The probiotic skin care product according to any of claims 3 to 5, characterized in that it further comprises: human epidermal growth factor of more than 1000 IU/mL.
7. The probiotic skin care product according to claim 6, wherein the human epidermal growth factor is recombinant human epidermal growth factor, and the dosage is 1500-.
8. A method of preparing a probiotic skin care product according to any one of claims 3 to 7, comprising:
mixing active probiotic lyophilized powder containing Lactobacillus rhamnosus and Lactobacillus delbrueckii with physiological saline, and optionally adding human epidermal growth factor to obtain liquid probiotic skin care product.
9. The method according to claim 8, wherein the liquid probiotic skin care product is further formulated as a moisturizing spray or mask product.
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Cited By (1)
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