CN112753580A - Method for regenerating plants by using wild rice callus and application - Google Patents
Method for regenerating plants by using wild rice callus and application Download PDFInfo
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- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention belongs to the technical field of plant breeding, and particularly relates to a method for regenerating plants by wild rice callus and application of the method. The invention utilizes plant tissue culture technology to perform callus induction on mature seeds of the wild rice, uses an improved basic culture medium, greatly improves the callus induction rate, uses an optimized callus multiplication culture medium to perform subculture multiplication on the callus to obtain more compact callus small particles, and then uses an optimized differentiation culture medium to regenerate the callus.
Description
Technical Field
The invention belongs to the technical field of plant breeding, and particularly relates to a method for regenerating plants by wild rice callus and application of the method.
Background
Chinese wild rice (Chinese wild rice) is a caryopsis of Chinese wild rice (Zizania latifolia), is recorded in Zhou Li and is one of ancient six cereals (rice, millet, sorghum, wheat and (wild rice)) in China as a special crop with a long history. After the Tang dynasty, with the great increase of population, the popularization of various farming activities such as lake-surrounding field building and rice planting technologies, and the difficulty in harvesting and shelling the wild rice, the wild rice gradually fades out of sight of people. Zhai becomes Kai and other research statistics show that wild rice grows in other areas of China except areas with higher altitude such as Xinjiang and Tibet, and lakes are more dense in river basins of Huai river, middle and lower reaches of Yangtze river, such as Jiangsu, Anhui and Zhejiang river. The stem and leaf of Zizania plant can be used as animal feed due to its high protein content, and Zizania plant can also be used as biofuel, such as source for producing bioethanol. The zizania plant can absorb a large amount of nutrient substances in lakes due to the strong root system of the zizania plant, blocks the overgrowth of various algae due to sufficient nutrition, is favorable for preventing and treating the eutrophication of lakes and rivers, and the root of the zizania plant can be used as a traditional Chinese medicinal material and is a plant with higher economic value. When the wild rice plant is infected by the Ustilago latifolia (Ustilago esculenta), the stem base part of the wild rice plant continuously expands and is gradually domesticated to become the second aquatic vegetable of China, namely the wild rice stem. Therefore, harvesting the water vegetable zizania aquatica is the main form of zizania aquatica resource utilization in China at present, and the zizania aquatica rice which is native to China is rarely known.
Researches at home and abroad find that the wild rice has high nutritional value. The results of research on the nutritional ingredients of the wild rice in China, namely Dianthus hai Kai and the like show that the protein content of the wild rice in China can reach 13.2%, the essential amino acid structure of the wild rice is reasonable and rich, particularly the wild rice is rich in lysine and methionine, compared with the common rice, the wild rice has higher amino acid content, and the amino acid score of the wild rice is similar to that of milk powder and is obviously higher than that of common beans and cereals. The wild rice in China also contains high content of vitamins, such as B vitamins (vitamin B1, B2) and vitamin E; meanwhile, the fertilizer is rich in inorganic salts and trace elements, such as zinc, potassium, cobalt, manganese, iron, lithium, nickel, copper, sodium, magnesium, chromium and phosphorus; compared with rice, the rice is obviously rich in phosphorus, magnesium and sodium elements. Researches of Przybylski and the like find that the lipid content of the zizania latifolia rice is about 0.7-1.1%, the zizania latifolia rice contains 55.6-66.5% of essential fatty acid, the content of the essential fatty acid is obviously higher than that of the brown rice (36.9-39.1%), and the omega-3 fatty acid content of the zizania latifolia rice is 18 times that of the brown rice; it has been shown that lipids of aeginetia indica rice are excellent sources of linoleic acid and linolenic acid, and particularly, linolenic acid is abundantly present in aeginetia indica rice. Klensporf-Pawlik and Aladedurye indicate that the wild rice has a low lipid content, the lipid content of Chinese and Japanese wild rice is 1.1% and 1.4%, respectively, while the lipid content of conventional white rice or brown rice is between 2.6% and 2.8%. Jinxin and Canbove researches find that the total starch content in the wild rice in China reaches 65.74%, the resistant starch content in the total starch accounts for 11.37%, the amylose content accounts for 8.37%, and meanwhile, the total dietary fiber content reaches 7.24%, wherein the soluble dietary fiber accounts for 1.97%, and the insoluble dietary fiber accounts for 5.2%. The biological activity and health care value of the wild rice are proved to comprise antioxidant activity, improvement of insulin resistance, improvement of lipid toxicity, prevention of cardiovascular diseases and the like. Food science research proves that the wild rice has low content of arsenic, cadmium, lead and the like, and animal experiments and population investigation prove that the wild rice is safe to eat. The wild rice can be mixed with other food for application. As a whole grain food, the wild rice is a related genus of rice, and the excellent quality of the wild rice has important practical application value on the improvement of the rice quality and resistance. It is known that at present, domestic researchers develop the hybridization of wild rice and rice, and obtain a new wild rice strain with higher yield.
The zizania latifolia as a grain crop with extremely high nutritive value has some self limitations, for example, the zizania latifolia contains a shattering gene, so that mature seeds are easy to shatter and the like, and the zizania latifolia is not suitable for large-scale production and application, so that the function research of the zizania latifolia related gene is particularly important, the related technology and research of the zizania latifolia gene engineering are urgently broken through, the establishment of a zizania latifolia tissue culture regeneration system is the basis of the gene engineering research, however, the currently reported methods for the zizania latifolia regeneration include 3, the first method is to use a tender bud of a zizania latifolia plant as an explant, on one hand, the material is limited, and on the other hand, the zizan; the second is direct regeneration of the young embryo, on one hand, the material is limited, on the other hand, the method is not suitable for agrobacterium-mediated genetic transformation, and has no significance for the research of the wild rice genetic engineering; the other method is to try to use the mature seeds of the wild rice as explants to induce callus and then differentiate the callus into seedlings, undoubtedly, the regeneration system is more suitable for agrobacterium-mediated genetic transformation, however, the differentiation culture medium in the research is not suitable for the differentiation of the wild rice seedlings, only the differentiated root systems are obtained, but the differentiation culture medium cannot differentiate the buds, and finally, the regeneration plants are not obtained. Therefore, the problem of the regeneration of the wild rice plants by callus is urgently needed to be solved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for regenerating a plant from wild rice callus and application thereof, and aims to solve part of the problems in the prior art or at least alleviate part of the problems in the prior art.
The invention is realized in such a way that a method for regenerating plants by wild rice callus comprises the following steps:
taking mature wild rice seeds as a tissue culture explant; selecting an optimized induction culture medium to induce the callus, then carrying out subculture proliferation on the callus through the optimized proliferation culture medium, and then differentiating the callus into a new plant by using the optimized differentiation culture medium;
the optimized induction culture medium is added into an improved minimal culture medium at a concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-12,4-D, pH 5.8-6.0;
the optimized multiplication culture medium is added into an improved minimal culture medium at a concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 15 mg.L-12,4-D, pH 5.8-6.0;
the optimized differentiation culture medium is added into an improved minimal medium with the concentration of 30 g.L-1Sucrose (g) 30 g. L-1Sorbitol, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-16-BA, 1 mg. L-1NAA of (4), pH 5.8-6.0;
the improved minimal medium formula (mg/L): (NH)4)2SO4 463；KNO3 2830；CaCl2·2H2O 166；MgSO4·7H2O 185；KH2PO4 400；KI 0.75；H3BO3 3；MnSO4·4H2O 13.2；MnSO4·H2O 10；ZnSO4·7H2O 2；Na2MoO4·2H2O 0.25；CuSO4·5H2O 0.025；CoCl2·6H2O 0.025；Myo-inositol 100；Nicotinic acid 1；Pyridoxine HCl 1；Thiamine HCl 10；Na2EDTA·2H2O 41.3；FeSO4·7H2O 27.8。
Further, the mature wild rice seeds are disinfected before callus induction.
Further, the culture conditions for callus induction were: culturing with light at 4000lux intensity and light cycle of 16/8h at 28 + -2 deg.C for 3 weeks to obtain embryogenic callus.
Further, the culture conditions for callus proliferation were: light culture, light intensity of 4000lux, light period of 16/8h, and temperature of 28 +/-2 ℃.
Further, the culture conditions of the differentiation stage are light culture, the light intensity is 4000lux, the light period is 16/8h, the temperature is 28 +/-2 ℃, and differentiated seedlings can be seen in 50-60 days.
Further, inoculating the differentiated seedling to rooting and strong seedling culture medium under aseptic condition, wherein the basic culture medium is 1/2MS culture medium, and adding 2 mg.L-1Vitamin B5, 20 g.L-1Sucrose, 5-10 g.L-1Agar of (1 mg. L)-1NAA of (5), pH 5.8-6.0, culture conditions: light culture with light intensity of 4000lux, light period of 16/8h, temperature of 28 + -2 deg.C, seedling height of 5-15 cm after 2-3 weeks.
The invention also provides application of the method for regenerating the wild rice callus in the wild rice callus regeneration plant.
In summary, the advantages and positive effects of the invention are:
the invention utilizes plant tissue culture technology to perform callus induction on the mature seeds of the wild rice, and uses the improved basic culture medium, thereby greatly improving the callus induction rate with the callus induction rate as high as 100 percent. Meanwhile, the optimized callus proliferation culture medium is used for carrying out subculture proliferation on the callus, the using amount of auxin 2,4-D is increased in stages to obtain more compact callus small particles, then the optimized differentiation culture medium is used for regenerating plants from the callus, explants used in the method are convenient to obtain, stored mature seeds are taken at any time and are not limited by seasons, predecessors use a common MS culture medium to differentiate the zizania micrantha callus to obtain more root systems, the differentiation of the buds is difficult, the differentiation of the buds is not seen all the time, the robust regeneration plants can be obtained only in 3 months through the optimized differentiation culture medium, more importantly, the embryogenic callus is a high-efficiency explant infected by agrobacterium, and the establishment of a callus regeneration plant system lays a solid experimental foundation for the establishment of a genetic transformation system of the callus regeneration plant system.
Drawings
FIG. 1 is a graph of the effect of wild rice callus induction;
FIG. 2 is a graph of effect of wild rice callus proliferation;
FIG. 3 is a diagram of the regeneration effect of black rice seedlings;
FIG. 4 is a diagram of the effect of rooting and strengthening seedlings of wild rice.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the equipment and reagents used in the examples and test examples are commercially available without specific reference. The specific embodiments described herein are merely illustrative of the invention and are not intended to be limiting.
Various modifications to the precise description of the invention will be readily apparent to those skilled in the art from the information contained herein without departing from the spirit and scope of the appended claims. It is to be understood that the scope of the invention is not limited to the procedures, properties, or components defined, as these embodiments, as well as others described, are intended to be merely illustrative of particular aspects of the invention. Indeed, various modifications of the embodiments of the invention which are obvious to those skilled in the art or related fields are intended to be covered by the scope of the appended claims.
For a better understanding of the invention, and not as a limitation on the scope thereof, all numbers expressing quantities, percentages, and other numerical values used in this application are to be understood as being modified in all instances by the term "about". Accordingly, unless expressly indicated otherwise, the numerical parameters set forth in the specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained. At the very least, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. In the present invention, "about" means within 10%, preferably within 5% of a given value or range.
The normal temperature in the following embodiments of the present invention refers to a natural room temperature condition in four seasons, and is not subjected to additional cooling or heating treatment, and is generally controlled at 10 to 30 ℃, preferably 15 to 25 ℃.
The invention discloses a method for regenerating a plant from wild rice callus and application thereof. The improved culture medium (without agar and sucrose) formula (mg/L) involved in the invention is as follows: (NH)4)2SO4 463；KNO3 2830；CaCl2·2H2O 166；MgSO4·7H2O 185；KH2PO4 400；KI 0.75；H3BO3 3；MnSO4·4H2O 13.2；MnSO4·H2O 10；ZnSO4·7H2O 2；Na2MoO4·2H2O 0.25；CuSO4·5H2O 0.025；CoCl2·6H2O 0.025；Myo-inositol 100；Nicotinic acid 1；Pyridoxine HCl 1；Thiamine HCl 10；Na2EDTA·2H2O 41.3；FeSO4·7H2And (3) O27.8. The details are shown in the following examples.
Examples
1. Callus induction and proliferation:
1) seed disinfection: putting mature seeds of wild rice into a 50ml sterilizing centrifuge tube, sterilizing for 1min by using ethanol with the volume ratio of 70%, shaking continuously during the period, discarding waste liquid, sterilizing by using sodium hypochlorite solution with the effective chlorine concentration of 1%, putting the sterilized seeds into a constant-temperature oscillation incubator at 37 ℃ during the period, oscillating for 40min at the rotating speed of 180r/min, discarding the waste liquid, and cleaning for 10 times by using sterilized water until the seeds are clarified.
2) Callus induction: placing the sterilized seeds in a sterilized culture dish, placing the sterilized seeds in front of a magnifying glass, and cutting off the embryo part of the seeds by using a sterilized scalpelAnd inoculating the strain in a callus induction culture medium under a sterile condition. Improved minimal medium with addition concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-12,4-D, pH 5.8-6.0; the culture conditions are as follows: culturing with light at 4000lux intensity, light period of 16/8h, and temperature of 28 + -2 deg.C for 3 weeks to obtain embryogenic callus with callus induction rate up to 90%, as shown in FIG. 1.
3) Callus proliferation: after three weeks, the induced callus was transferred under sterile conditions to a callus proliferation medium and proliferation culture continued, during which high concentrations of 2,4-D were added specifically to promote the state of callus, resulting in more compact callus granules, FIG. 2. Improved minimal medium with addition concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 15 mg.L-12,4-D, pH 5.8-6.0; the culture conditions are as follows: light culture, light intensity of 4000lux, light period of 16/8h, and temperature of 28 +/-2 ℃.
2. Seedling differentiation; after two weeks of propagation culture, the callus is inoculated to an optimized seedling differentiation culture medium under the aseptic condition, and a regeneration plant can be obtained in about 50-60 days. Since the black rice callus is easy to brown in the tissue culture process, the fresh culture medium is replaced every 3 weeks, sorbitol is added into the culture medium, so that the differentiation rate of the callus is improved, and the picture 3 shows that the differentiation rate of the callus is increased. Improved minimal medium with addition concentration of 30 g.L-1Sucrose (g) 30 g. L-1Sorbitol, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-16-BA, 1 mg. L-1NAA of (4), pH 5.8-6.0; the culture conditions are as follows: culturing with light at 4000lux intensity and light period of 16/8h, and maintaining at 28 + -2 deg.C for 50-60 days to obtain differentiated seedling.
3. Rooting and strengthening seedlings; taking out the differentiated regenerated plant from the original culture medium, and inoculating to rooting and seedling strengthening culture under aseptic conditionThe culture medium is 1/2MS culture medium, and 2 mg.L is added-1Vitamin B5, 20 g.L-1Sucrose, 5-10 g.L-1Agar of (1 mg. L)-1NAA of (4), pH 5.8-6.0, culture conditions: light culture with light intensity of 4000lux, light period of 16/8h, temperature of 28 + -2 deg.C, seedling height of 5-15 cm after 2-3 weeks, figure 4.
4. Transplanting: taking out the strong seedlings from the culture medium, washing the root culture medium with warm water, and transplanting the seedlings into the field, wherein the survival rate can reach more than 95%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. A method for regenerating plants from wild rice callus is characterized by comprising the following steps:
taking mature wild rice seeds as a tissue culture explant; selecting an optimized induction culture medium to induce the callus, then carrying out subculture proliferation on the callus through the optimized proliferation culture medium, and then differentiating the callus into a new plant by using the optimized differentiation culture medium;
the optimized induction culture medium is added into an improved minimal culture medium at a concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-12,4-D, pH 5.8-6.0;
the optimized multiplication culture medium is added into an improved minimal culture medium at a concentration of 30 g.L-1Sucrose, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 15 mg.L-12,4-D, pH 5.8-6.0;
the optimized differentiation culture medium is added into an improved minimal medium with the concentration of 30 g.L-1Sucrose (g) 30 g. L-1Of (D) sorbAlcohol, 5-10 g.L-10.5 g.L of agar-1L-glutamine, 0.5 g.L-10.5 g.L of L-proline-1Casein, 2 mg. L-16-BA, 1 mg. L-1NAA of (4), pH 5.8-6.0;
the improved minimal medium formula (mg/L): (NH)4)2SO4 463；KNO3 2830；CaCl2·2H2O 166；MgSO4·7H2O 185；KH2PO4 400；KI 0.75；H3BO3 3；MnSO4·4H2O 13.2；MnSO4·H2O 10；ZnSO4·7H2O 2；Na2MoO4·2H2O 0.25；CuSO4·5H2O 0.025；CoCl2·6H2O 0.025；Myo-inositol 100；Nicotinic acid 1；Pyridoxine HCl 1；Thiamine HCl 10；Na2EDTA·2H2O 41.3；FeSO4·7H2O 27.8。
2. The method for regenerating a plant from wild rice callus as claimed in claim 1, wherein the method comprises the steps of: the mature wild rice seeds are disinfected before callus induction.
3. The method for regenerating a plant from wild rice callus as claimed in claim 1, wherein the method comprises the steps of: the culture conditions for callus induction were: culturing with light at 4000lux intensity and light cycle of 16/8h at 28 + -2 deg.C for 3 weeks to obtain embryogenic callus.
4. The method for regenerating a plant from wild rice callus as claimed in claim 1, wherein the method comprises the steps of: the culture conditions for callus proliferation are as follows: light culture, light intensity of 4000lux, light period of 16/8h, and temperature of 28 +/-2 ℃.
5. The method for regenerating a plant from wild rice callus as claimed in claim 1, wherein the method comprises the steps of: the culture conditions of the differentiation stage are light culture, the light intensity is 4000lux, the light cycle is 16/8h, the temperature is 28 +/-2 ℃, and differentiated seedlings can be seen in 50-60 days.
6. The method for regenerating a plant from wild rice callus as claimed in claim 5, wherein the method comprises the steps of: inoculating the differentiated seedling to rooting and seedling strengthening culture medium under aseptic condition, wherein the basic culture medium is 1/2MS culture medium, and adding 2 mg.L-1Vitamin B5, 20 g.L-1Sucrose, 5-10 g.L-1Agar of (1 mg. L)-1NAA of (5), pH 5.8-6.0, culture conditions: light culture with light intensity of 4000lux, light period of 16/8h, temperature of 28 + -2 deg.C, seedling height of 5-15 cm after 2-3 weeks.
7. The use of the method of any one of claims 1 to 6 for the regeneration of wild rice callus in a plant.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637701A (en) * | 2021-09-10 | 2021-11-12 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for establishing wild rice genetic transformation system |
| CN116837024A (en) * | 2023-08-09 | 2023-10-03 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for carrying out gene expression by utilizing agrobacterium to transiently transform wild rice seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109691390A (en) * | 2019-02-28 | 2019-04-30 | 浙江师范大学 | Wild rice stem seed callus cultivation |
-
2021
- 2021-01-29 CN CN202110134170.9A patent/CN112753580B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109691390A (en) * | 2019-02-28 | 2019-04-30 | 浙江师范大学 | Wild rice stem seed callus cultivation |
Non-Patent Citations (3)
| Title |
|---|
| 于洋等: "广适应性籼稻愈伤组织诱导培养基的筛选", 《西南农业学报》 * |
| 薛建平等: "《植物组织培养与工厂化种苗生产技术》", 30 November 2013 * |
| 袁隆平等: "《第三代杂交水稻育种技术》", 30 September 2019 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637701A (en) * | 2021-09-10 | 2021-11-12 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for establishing wild rice genetic transformation system |
| CN116837024A (en) * | 2023-08-09 | 2023-10-03 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for carrying out gene expression by utilizing agrobacterium to transiently transform wild rice seeds |
| CN116837024B (en) * | 2023-08-09 | 2024-02-02 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for carrying out gene expression by utilizing agrobacterium to transiently transform wild rice seeds |
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