CN112741051A - Method for collecting pure mature larvae of cecidomyiia mangifera - Google Patents
Method for collecting pure mature larvae of cecidomyiia mangifera Download PDFInfo
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- CN112741051A CN112741051A CN202110113229.6A CN202110113229A CN112741051A CN 112741051 A CN112741051 A CN 112741051A CN 202110113229 A CN202110113229 A CN 202110113229A CN 112741051 A CN112741051 A CN 112741051A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for collecting pure mature larvae of cecidomyiia. Belongs to the technical field of agricultural pest control. The method comprises the following steps: collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and placing the breeding sieve in a feeding basin for breeding, wherein fine soil is arranged in the feeding basin; feeding the seeds under the conditions that the temperature is 22-25 ℃ and the relative humidity is 80-90%. The invention utilizes the characteristic that the cecidomyiia is used for enabling the aged larvae to penetrate into the soil to pupate, the breeding device is optimized, fine soil is used as a bedding medium to be used as pupating soil, when the aged larvae are drilled out from host substances and fall into the bedding medium, the aged larvae are screened out by a mesh screen, and thus a large amount of pure aged larvae are collected.
Description
Technical Field
The invention relates to the technical field of agricultural pest control, in particular to a method for collecting pure mature larvae of cecrophycidae indica.
Background
The cerbera manghas gall midge is an important pest for killing cerbera manghas. The cerbera manghas gall midge pests can damage cerbera manghas leaves, flowers and young fruits. The young leaf of the new shoot is the gall midge of mango leaf, and the young flower and the young fruit are the common gall midge of mango. A large number of cecidomyiia old larvae are collected for biological research, and the method has very important significance for preventing and controlling the cecidomyiia old larvae.
In scientific research, a large number of pure mature larvae are needed for biological characteristics, ecological research, pest control experiments and the like, so that the method for efficiently collecting the pure mature larvae of the cecrophycidae midge provides a basis for related research.
However, in the biological research and artificial propagation process, the large collection of the amaurosis amadori mature larvae is quite difficult. The common method is that host substances of the aged larvae of the mangifera indica cecidomyiis are collected and put into a transparent plastic bag, the aged larvae jump into the plastic bag after being drilled out of the host substances, the aged larvae are picked out by a brush pen, the working efficiency is low, and the aged larvae are not neat in age; the plastic bag filled with the gall midge mature larvae can be washed by water and then filtered by the gauze, the filtered impurities are too much, the separation of the gall midge mature larvae is difficult, and the pure mature larvae are not easy to obtain.
Therefore, how to provide a method for collecting the pure mature larvae of the cerbera manghas is a problem which needs to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a method for collecting the pure mature larvae of the gall midge. The invention uses fine soil medium as a bedding to be placed in a feeding basin, after the old larvae of the mango gall midge are drilled out from host substances, the mango gall midge jumps into the medium, the old larvae are screened out by a mesh screen, the old larvae of the mango gall midge are effectively separated from the host substance medium, and the pure old larvae of the mango gall midge are collected for research.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for collecting old larvae of cecidomyiia Mangifera comprises the following steps:
(1) collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and putting the host substances into a feeding basin for breeding, wherein fine soil is arranged in the feeding basin;
the cultivation temperature is 22-25 ℃, the relative humidity is 80-90%, and the cultivation time is 1-7 d;
(2) and after the cultivation is finished, insect and soil separation is carried out, and the pure cecropin gall midge mature larva is collected.
Has the advantages that: after one week of the next day, the old larvae of the mango gall midge come out of the host substance, fall into the breeding pot from the net bag through the breeding sieve, and then the old larvae are screened out by the net sieve, so that a large amount of pure old larvae of the mango gall midge can be collected.
Preferably: the species of the cerbera manghas comprise two species of the cerbera manghas poppy manghas and the cerbera manghas leaf gall midge.
Has the advantages that: can prevent other impurities in the host substance from falling into the feeding basin, ensure that only the mature larvae fall into the feeding basin, and improve the working efficiency for subsequent separation of the larvae and soil.
Preferably: the mesh bag is a gauze with 20 meshes.
Preferably: the mesh bag is a rectangular bag with the shape of 30 multiplied by 35cm, and a closing rope is arranged at the opening of the mesh bag.
Has the advantages that: the mesh bag with the closing rope can tighten the host substances respectively loaded in the mesh bag, and the quantitative host substances with better mesh bag size can be set and can be placed in the feeding sieve.
Preferably: the mango gall midge host substances comprise mango spica, young shoots and young leaves.
Has the advantages that: collecting host substances needing to be collected from the mature larvae of the acrossodes canicola as damaged scions of the Cerbera Manghas; host substances collected by the aged larvae of the cecidomyiia mangifera are damaged young tips and young leaves, and a large number of target insects can be collected by adopting the method.
Preferably: principle of collection: according to the requirements, host substances of the old larvae of the ceberella crassipes collected in a classified manner are respectively put into net bags according to the types of the ceberella crassipes, wherein the spikes of the ceberella crassipes are independently bagged; mixing young shoots and young leaves, and bagging.
Preferably: the feeding sieve is a circular vegetable sieve with the diameter of 35-38 cm and the aperture of 0.5-1 cm.
Further: the feeding sieve and the feeding basin are made of common plastic, and the color of the feeding basin is blue, so that gall midge bodies can be observed easily.
Preferably: the humidity of the host substance is regulated by the following method: when the humidity is more than or equal to 90 percent, a gauze is selected to cover the feeding basin; when the humidity is less than 90%, the feeding basin cover is selected to cover the feeding basin.
Has the advantages that: when the humidity is more than or equal to 90 percent, the feeding basin is covered by a gauze, the phenomenon that water drops are formed on the wall of the feeding basin due to overhigh humidity, and the aged larvae of the cecropins jump onto the water drops, which affects the health of the larvae and brings inconvenience to the collection of the aged larvae is avoided. When the feeding basin is wet, the aged larvae stuck on the feeding basin are difficult to pick out by using a writing brush, and the feeding basin wastes time and labor; when the humidity is less than 90%, the breeding pot cover covers the breeding pot, so that the humidity of the host substance and the breeding pot can be maintained, and the aged larvae can normally develop in the host substance and safely fall into the soil of the breeding pot.
Preferably: sieving fine soil with 40 mesh sieve before use, and sterilizing at 120 deg.C for 12 hr; when in use, the water content is adjusted to 5% by sterile water, and the mixture is spread in a feeding basin.
Has the advantages that: the fine soil is sterilized at high temperature and adjusted to the ground by sterile water before use, the habitat of the aged larvae of the Cerbera Manghas is improved, the water content of the fine soil paved in the feeding basin is set to be 5%, the humidity in the feeding basin is neutralized, the humidity required by the aged larvae of the Cerbera Manghas in the fine soil is met, the aged larvae can not immediately form cocoons after falling into the fine soil, or the fine soil is too wet to form particles and is difficult to separate and screen out from the aged larvae.
Preferably: and (2) separating the insect soil into circular meshes with 40 meshes.
According to the technical scheme, compared with the prior art, the method for collecting the pure aged larvae of the mango gall midges disclosed by the invention has the advantages that the characteristics that the mango gall midges enable the aged larvae to penetrate into soil to pupate are utilized, feeding appliances are optimized, fine soil is used as a bedding medium to pupate soil, when the aged larvae are drilled out from host substances and fall into the bedding medium, the aged larvae are screened out by using a mesh screen, and therefore a large amount of pure aged larvae are collected.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a method for collecting pure mature larvae of cecrophycidae indica.
In the examples, the required tools and raw materials were collected from commercial sources, and the method not mentioned is a conventional process and will not be described further.
Example 1
A method for collecting old larvae of cecidomyiia Mangifera comprises the following steps:
(1) collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and putting the host substances into a feeding basin for breeding, wherein fine soil is arranged in the feeding basin;
the cultivation temperature is 22 ℃, the relative humidity is 80%, and the cultivation time is 3-7 d;
(2) and after the cultivation is finished, insect and soil separation is carried out, and the pure cecropin gall midge mature larva is collected.
In order to further optimize the technical scheme: the species of the cerbera manghas comprise two species of the cerbera manghas poppy manghas and the cerbera manghas leaf gall midge.
In order to further optimize the technical scheme: the mesh bag is a gauze with 20 meshes.
In order to further optimize the technical scheme: the mesh bag is a rectangular bag with the shape of 30 multiplied by 35cm, and a closing rope is arranged at the opening of the mesh bag.
In order to further optimize the technical scheme: the mango gall midge host substances comprise mango spica, young shoots and young leaves.
In order to further optimize the technical scheme: principle of collection: according to the requirements, host substances of the old larvae of the ceberella crassipes collected in a classified manner are respectively put into net bags according to the types of the ceberella crassipes, wherein the spikes of the ceberella crassipes are independently bagged; mixing young shoots and young leaves, and bagging.
In order to further optimize the technical scheme: the feeding sieve is a circular vegetable sieve with the diameter of 35cm and the aperture of 0.5 cm.
In order to further optimize the technical scheme: the humidity of the host substance is regulated by the following method: when the humidity is more than or equal to 90 percent, a gauze is selected to cover the feeding basin; when the humidity is less than 90%, the feeding basin cover is selected to cover the feeding basin.
In order to further optimize the technical scheme: sieving fine soil with 40 mesh sieve before use, and sterilizing at 120 deg.C for 12 hr; when in use, the water content is adjusted to 5% by sterile water, and the mixture is spread in a feeding basin.
In order to further optimize the technical scheme: and (2) separating the insect soil into circular meshes with 40 meshes.
Example 2
A method for collecting old larvae of cecidomyiia Mangifera comprises the following steps:
(1) collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and putting the host substances into a feeding basin for breeding, wherein fine soil is arranged in the feeding basin;
the cultivation temperature is 23 ℃, the relative humidity is 85%, and the cultivation time is 2-6 d;
(2) and after the cultivation is finished, insect and soil separation is carried out, and the pure cecropin gall midge mature larva is collected.
In order to further optimize the technical scheme: the species of the cerbera manghas comprise two species of the cerbera manghas poppy manghas and the cerbera manghas leaf gall midge.
In order to further optimize the technical scheme: the mesh bag is a gauze with 20 meshes.
In order to further optimize the technical scheme: the mesh bag is a rectangular bag with the shape of 30 multiplied by 35cm, and a closing rope is arranged at the opening of the mesh bag.
In order to further optimize the technical scheme: the mango gall midge host substances comprise mango spica, young shoots and young leaves.
In order to further optimize the technical scheme: principle of collection: according to the requirements, host substances of the old larvae of the ceberella crassipes collected in a classified manner are respectively put into net bags according to the types of the ceberella crassipes, wherein the spikes of the ceberella crassipes are independently bagged; mixing young shoots and young leaves, and bagging.
In order to further optimize the technical scheme: the breeding sieve is a circular vegetable sieve with diameter of 36cm and aperture of 0.7 cm.
In order to further optimize the technical scheme: the humidity of the host substance is regulated by the following method: when the humidity is more than or equal to 90 percent, a gauze is selected to cover the feeding basin; when the humidity is less than 90%, the feeding basin cover is selected to cover the feeding basin.
In order to further optimize the technical scheme: sieving fine soil with 40 mesh sieve before use, and sterilizing at 120 deg.C for 12 hr; when in use, the water content is adjusted to 5% by sterile water, and the mixture is spread in a feeding basin.
In order to further optimize the technical scheme: and (2) separating the insect soil into circular meshes with 40 meshes.
Example 3
A method for collecting old larvae of cecidomyiia Mangifera comprises the following steps:
(1) collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and putting the host substances into a feeding basin for breeding, wherein fine soil is arranged in the feeding basin;
the cultivation temperature is 25 ℃, the relative humidity is 90%, and the cultivation time is 1-4 d;
(2) and after the cultivation is finished, insect and soil separation is carried out, and the pure cecropin gall midge mature larva is collected.
In order to further optimize the technical scheme: the species of the cerbera manghas comprise two species of the cerbera manghas poppy manghas and the cerbera manghas leaf gall midge.
In order to further optimize the technical scheme: the mesh bag is a gauze with 20 meshes.
In order to further optimize the technical scheme: the mesh bag is a rectangular bag with the shape of 30 multiplied by 35cm, and a closing rope is arranged at the opening of the mesh bag.
In order to further optimize the technical scheme: the mango gall midge host substances comprise mango spica, young shoots and young leaves.
In order to further optimize the technical scheme: principle of collection: according to the requirements, host substances of the old larvae of the ceberella crassipes collected in a classified manner are respectively put into net bags according to the types of the ceberella crassipes, wherein the spikes of the ceberella crassipes are independently bagged; mixing young shoots and young leaves, and bagging.
In order to further optimize the technical scheme: the breeding sieve is a circular vegetable sieve with diameter of 38cm and aperture of 1 cm.
In order to further optimize the technical scheme: the humidity of the host substance is regulated by the following method: when the humidity is more than or equal to 90 percent, a gauze is selected to cover the feeding basin; when the humidity is less than 90%, the feeding basin cover is selected to cover the feeding basin.
In order to further optimize the technical scheme: sieving fine soil with 40 mesh sieve before use, and sterilizing at 120 deg.C for 12 hr; when in use, the water content is adjusted to 5% by sterile water, and the mixture is spread in a feeding basin.
In order to further optimize the technical scheme: and (2) separating the insect soil into circular meshes with 40 meshes.
Comparative test
The emergence results of the adult raised by the pure aged larvae of the cecrophycidae obtained by the methods of the embodiments 1 to 3 of the invention are compared with the emergence rates (water flushing and mesh screening) of the cecrophycidae obtained by the prior art.
TABLE 1 emergence rate of Cerbera Manghas under different treatment conditions
The result shows that the fine soil is used as a bedding medium, so that the pure mature larvae of the mango gall midge are effectively separated from host substances, the mature larvae of the mango gall midge are easily obtained, and the emergence rate of the mango gall midge reaches 91%. The prior art treatment method adopts water washing, mesh screen filtration or blank control, and the aged larvae have high humidity and cannot be used immediately. In addition, the blank-treated mature larvae are stuck in a rearing basin and are difficult to clean for use, the bodies of the mature larvae cleaned by a brush are easy to be injured, the mature larvae prefer to be stuck into a small group, the individuals are difficult to separate for use, the emergence rate is 85.9-87.2%, and is obviously lower than that of the pure mature larvae of the cecrodes opercules obtained by the method.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for collecting the old larvae of cecidomyiia Mangifera is characterized in that: the method comprises the following steps:
(1) collecting host substances of the old larvae of the cecidomyiia by using a mesh bag, putting the host substances into a feeding sieve, and putting the host substances into a feeding basin for breeding, wherein fine soil is arranged in the feeding basin;
the cultivation temperature is 22-25 ℃, the relative humidity is 80-90%, and the cultivation time is 1-7 d;
(2) and after the cultivation is finished, insect and soil separation is carried out, and the pure cecropin gall midge mature larva is collected.
2. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: the species of the cerbera manghas comprise two species of the common cerbera manghas and the cerbera manghas.
3. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: the mesh bag is a gauze with 20 meshes.
4. A method for collecting the old larva of Cerbera Manghas according to claim 3, characterized in that: the mesh bag is a rectangular bag with the shape of 30 multiplied by 35cm, and a closing rope is arranged at the bag opening.
5. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: the mango gall midge host substances comprise mango ears, young shoots and young leaves.
6. A method for collecting the old larva of Cerbera Manghas according to claim 5, wherein: the principle of collection: according to the requirements, host substances of the old larvae of the ceberella crassipes collected in a classified manner are respectively put into net bags according to the types of the ceberella crassipes, wherein the spikes of the ceberella crassipes are independently bagged; mixing young shoots and young leaves, and bagging.
7. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: the feeding sieve is a circular vegetable sieve with the diameter of 35-38 cm and the aperture of 0.5-1 cm.
8. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: the humidity adjusting method of the host substance comprises the following steps: when the humidity is more than or equal to 90 percent, a gauze is selected to cover the feeding basin; when the humidity is less than 90%, the feeding basin cover is selected to cover the feeding basin.
9. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: sieving the fine soil with a 40-mesh sieve before use, and sterilizing at 120 ℃ for 12 h; when in use, the water content is adjusted to 5% by sterile water, and the mixture is spread in a feeding basin.
10. The method for collecting the amadoria mangifera mature larvae according to claim 1, which comprises the following steps: and (3) separating the insect soil into circular meshes with meshes of 40 meshes.
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