CN112715672A - Oligosaccharide mother-emulsified infant formula milk and preparation method thereof - Google Patents
Oligosaccharide mother-emulsified infant formula milk and preparation method thereof Download PDFInfo
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- CN112715672A CN112715672A CN202011580887.8A CN202011580887A CN112715672A CN 112715672 A CN112715672 A CN 112715672A CN 202011580887 A CN202011580887 A CN 202011580887A CN 112715672 A CN112715672 A CN 112715672A
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- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1522—Inorganic additives, e.g. minerals, trace elements; Chlorination or fluoridation of milk; Organic salts or complexes of metals other than natrium or kalium; Calcium enrichment of milk
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Abstract
The invention discloses oligosaccharide breast-emulsified infant formula milk, which comprises the following raw materials in percentage by mass: 12.9-37.0% of raw milk, 2.0-8.88% of desalted whey powder, 0-2.08% of concentrated whey protein powder, 0.9-1.78% of anhydrous cream, 1.0-1.78% of blend vegetable oil, 0-25.6% of lactose, 0.48-1.0% of mother emulsified oligosaccharide composition, 0.045% of compound vitamin, 0.045% of compound mineral substance, 0.033-0.042% of buffer salt, 0.007-0.02% of choline, 0.006-0.015% of taurine and water for supplementing to 100%. The formula milk of the invention has carbohydrate more similar to breast milk, is more suitable for infants, and has convenient drinking and good stability.
Description
Technical Field
The invention belongs to the technical field of dairy products, and particularly relates to oligosaccharide breast-emulsified infant formula milk and a preparation method thereof.
Background
From birth to 12 months, infants are very fragile, have an imperfect digestive and metabolic system, but grow and develop at a very fast rate, so that the infants have a high demand for nutrients and need to be fed with foods that are nutritionally complete, balanced and digestible. The most desirable food for infants is breast milk. The breast milk contains various bioactive substances, and can change the immunity of human body, gastrointestinal tract and brain development. Breast milk can alleviate advanced metabolic disorders in infants, such as obesity and type 2 diabetes. The world health organization recommends that infants be breastfed purely 6 months prior to their life. The national health committee of the people's republic of China (the former Ministry of health of the people's republic of China) published 'human milk health literacy-basic knowledge and skills' in 2012, and the latest edition 'infant feeding guide' published by the Chinese nutrition institute in 2016 emphasize that 'human milk is the most ideal natural food for infants', advocate that infants are born with pure human milk for at least 6 months. Besides its nutritional advantages, breast feeding is both convenient and safe. However, due to various factors, many infants are still unable to receive breast feeding. Only 29.2% of infants in china achieve the recommendation of pure breast feeding, and only 38% worldwide. When breast feeding is not satisfactory, infant formula becomes the best breast feeding substitute for infants.
Currently, infant formula is mainly available on the market as infant formula. The history of simulating breast milk is the development process of developing infant formula milk powder in China. Its development can be summarized in four stages: simple conditioning phase, simple conditioning phase of major macronutrients (protein, fat, carbohydrate), active or functional component conditioning phase (fatty acid composition, amino acid composition, lactoferrin, breast milk oligosaccharide choline, etc.), micro-emulsification phase. Gradually, it is close to, but far from producing exactly the same infant formula as, but we are approaching to breast milk indefinitely. At present, most of the middle-high-end infant formula milk powder market which is closer to the breast milk is still firmly occupied by foreign brands. Therefore, the development of high quality infant formulas is of great social and economic importance.
Infant formula is different from ordinary food and has very high requirements on formula, production process, sanitation requirement and the like. Since infant formula is intended to replace breast milk, the goal is to have its nutritional ingredients as close as possible to that of breast milk. For example, most infant formula lipids mimic breast milk in several characteristic dimensions, primarily from fatty acids, fats and other complex lipids; the protein simulates breast milk mainly from amino acid, whey protein and casein ratio, lactoferrin, lactalbumin and the like, and the carbohydrate is lactose mainly. With the growing awareness of nutritional science, the understanding of carbohydrate functions in infant food and the deep knowledge of breast milk ingredients, non-digestible carbohydrate (fructo-oligosaccharides, FOS, galacto-oligosaccharides GOS) formulas have been developed to provide better digestible formulas for infants, and the formula design and nutritional ingredients of infant formulas have been greatly improved. Although the addition of GOS, FOS and other components can simulate breast milk oligosaccharides, the GOS, the FOS and other components are not HMOs and cannot completely replace the functions of the HMOs. Compared with these oligosaccharides, HMOs have unique functions of supporting colonization of intestinal flora, inhibiting pathogen infection (necrotizing enterocolitis), promoting immunity and development, etc. Also, infant formulas are ultimately produced in the form of formula powders by spray drying processes because the powders are easier to store and transport. In recent years, with the health of the cold chain and the improvement of consumption level, the infant formula liquid milk begins to appear in foreign markets, and has the characteristics that the infant formula liquid milk can be directly eaten by infants without being mixed. The production process of infant formula liquid milk is characterized by that various raw and auxiliary materials are directly filled after being mixed and sterilized, and do not undergo the heat treatment processes of concentration and spray drying, so that its nutrient component heat treatment degree is less than that of infant formula milk powder.
Disclosure of Invention
The invention aims to solve the technical problem of providing more breast-emulsified infant formula. The infant formula milk enriches the nutrition and variety of infant formula food, and is a high-quality food which can be directly eaten by infants without being mixed with water. In order to achieve the purpose, the invention adopts the following technical scheme:
in one aspect, the invention provides an oligosaccharide breast milk formula for infants, which comprises the following raw materials in percentage by mass: 12.9-37.0% of raw milk, 2.0-8.88% of desalted whey powder, 0-2.08% of concentrated whey protein powder, 0.9-1.78% of anhydrous cream, 1.0-1.78% of blend vegetable oil, 0-25.6% of lactose, 0.48-1.0% of mother emulsified oligosaccharide composition, 0.045% of compound vitamin, 0.045% of compound mineral substance, 0.033-0.042% of buffer salt, 0.007-0.02% of choline, 0.006-0.015% of taurine and water for supplementing to 100%.
Further, the present invention provides a method of making a masterbatch oligosaccharide composition comprising: 2' -fucosyllactose, fructo-oligosaccharide, and galacto-oligosaccharide.
Further, the buffer salts include citric acid and calcium hydroxide.
Further, the blended vegetable oil is a compound raw material formed by uniformly mixing soybean oil, corn oil and rapeseed oil.
Further, the compound vitamin is a compound raw material formed by uniformly mixing a plurality of vitamin monomers;
the compound mineral substance is a compound raw material formed by uniformly mixing a plurality of mineral substance monomers.
In another aspect, the present invention provides a method for preparing an oligosaccharide breast-emulsified infant formula, comprising the steps of:
(1) dissolving the desalted whey powder, the concentrated whey protein powder, the lactose, the taurine, the choline and the master emulsification oligosaccharide composition in the pretreated raw milk, and uniformly mixing to obtain a mixed solution A;
(2) adding anhydrous cream and blend vegetable oil into the mixed solution A obtained in the step (1), and uniformly mixing to obtain mixed solution B;
(3) respectively dissolving the compound mineral substance and the compound vitamin in water to respectively obtain a mixed solution C and a mixed solution D;
(4) adding the mixed solution C and the mixed solution D obtained in the step (3) into the mixed solution B obtained in the step (2), uniformly mixing, and cooling to obtain a mixed solution E;
(5) dissolving buffer salt in water to obtain a mixed solution F;
(6) slowly adding the mixed solution F obtained in the step (5) into the mixed solution E obtained in the step (4), uniformly stirring and cooling to obtain a mixed solution G;
(7) and (4) adjusting the dry matter concentration of the mixed liquor G obtained in the step (6) to 12.50-14.50%, homogenizing and sterilizing to obtain the product.
Further, in the step (1), the temperature of the raw milk is 35-50 ℃, and the mixing time is 10-30 min;
the pretreatment is the standardization, homogenization and sterilization treatment of raw milk.
Further, in the step (2), the temperature of the anhydrous cream and the vegetable oil is 35-55 ℃, and the mixing time is 10-20 min;
in the step (3), the temperature of water is 15-45 ℃, and the concentration of the composite mineral substance after being dissolved in the water is 0.6-3.0%.
Further, in the step (4), cooling to 5-30 ℃;
in the step (5), the concentration of the buffer salt dissolved in water is 0.1-0.5%;
in the step (6), cooling to 5-30 ℃.
Further, in the step (7), the homogenization is a second homogenization, the pressure of the first homogenization is 15-19 MPa, the pressure of the second homogenization is 1-5 MPa, and the temperature of the two homogenization is 55-60 ℃.
And (4) performing ultrahigh-temperature instantaneous sterilization at the sterilization temperature of 130-134 ℃ for 2-5 seconds.
The positive progress effects of the invention are as follows:
(1) the infant formula milk is a liquid system infant formula food, is simplified in process without spray drying, and has the characteristics of better retention of nutrient components and convenience in drinking compared with common infant formula powder;
(2) the infant formula milk provided by the invention is unstable due to the liquid system containing various nutrient substances, and is easy to have the phenomena of fat floating, protein precipitation, color change and the like, and after the buffer salt system is added, the problems are solved, and the stability of the product quality is ensured;
(3) the content of oligosaccharide contained in the infant formula milk is simulated according to the carbon chain distribution of the oligosaccharide in breast milk, so that the carbohydrate is more similar to the breast milk, and the infant formula milk is more suitable for infants.
Drawings
The drawings are only for purposes of illustrating particular embodiments and are not to be construed as limiting the invention, wherein like reference numerals are used to designate like parts throughout.
FIG. 1 is a graph showing the value-added effect of a marbled oligosaccharide composition on Bifidobacterium Bb-12;
FIG. 2 is a graph showing the value-added effect of a feminized oligosaccharide composition on Lactobacillus fermentum CECT 5716.
Detailed Description
In order to more clearly illustrate the technical solution of the present invention, the technical solution of the present invention will be further illustrated with reference to the following specific embodiments:
one of the technical schemes provided by the invention is as follows:
the invention provides oligosaccharide breast-emulsified infant formula which comprises the following raw materials in percentage by mass: 12.9-37.0% of raw milk, 2.0-8.88% of desalted whey powder, 0-2.08% of concentrated whey protein powder, 0.9-1.78% of anhydrous cream, 1.0-1.78% of blend vegetable oil, 0-25.6% of lactose, 0.48-1.0% of mother emulsified oligosaccharide composition, 0.045% of compound vitamin, 0.045% of compound mineral substance, 0.033-0.042% of buffer salt, 0.007-0.02% of choline, 0.006-0.015% of taurine and water for supplementing to 100%.
The raw materials are all food grade and are commercially available.
A parent emulsified oligosaccharide composition comprising: 2' -fucosyllactose, fructo-oligosaccharide, and galacto-oligosaccharide. Specifically, the formula milk comprises 0.25-0.5% (w/w) of 2' -fucosyllactose, 0.20-0.45% (w/w) of fructo-oligosaccharide and 0.03-0.05% (w/w) of galacto-oligosaccharide.
Because the infant formula of the invention is a liquid system, the problems of fat aggregation, protein precipitation and the like are easily caused by the change of the pH value of the system, and experiments show that the problems of fat aggregation, protein precipitation and the like can be solved by adding the buffer salt of the invention.
Buffer salts include citric acid and calcium hydroxide. The specific addition amount is as follows: 0.013-0.017% (w/w) of citric acid and 0.02-0.025% (w/w) of calcium hydroxide.
The blended vegetable oil is a compound raw material formed by uniformly mixing soybean oil, corn oil and rapeseed oil.
The compound vitamin is a compound raw material formed by uniformly mixing a plurality of vitamin monomers.
The compound mineral substance is a compound raw material formed by uniformly mixing a plurality of mineral substance monomers.
The composition and usage range of the compound vitamins and compound minerals meet the requirement of reaching the required content of various vitamins and minerals in the final product, and the composition and usage amount of the vitamins and minerals are limited in the range of the vitamins and minerals of the final product. The composition and amount of vitamin complex and mineral complex can be adjusted by one skilled in the art according to the vitamin and mineral content of the final product of the invention.
The water in the feedstock is preferably deionized water.
The infant formula comprises the following nutrients per 100 kj: 0.43-0.72 g protein, 1.05-1.43 g fat, 2.2-3.3 g carbohydrate, 0.07-0.33 g linoleic acid, 12-30 mg linolenic acid, multivitamins (including 14-36 μ g RE vitamin A, 0.48-1.20 μ g vitamin D, 0.12-1.20 mg alpha-TE vitamin E, 0.96-6.45 μ g vitamin K1, 14-72 μ g vitamin B1, 19-120 μ g vitamin B2, 8.4-41.8 μ g vitamin B6, 0.024-0.359 μ g vitamin B12, 96-359 μ g nicotinic acid, 2.9-12.0 μ g folic acid, 96-359 μ g folic acid, 2.4-16.7 mg vitamin C, 0.36-2.39 μ g biotin, 4.8-23.9 mg choline, 14-12.0 μ g mineral (including 14.7-14 mg-12 mg sodium, 14.6-14 mg calcium, 14-6 mg calcium, 14.6-6 mg vitamin B36, 14-6 μ g vitamin B36 mg vitamin B6, calcium, 12-38 mg of chlorine, 0.72-2.06 mu g of selenium) and 0.8-4.0 mg of taurine.
The infant formula milk is suitable for infants of 0-6 months of age.
The second technical scheme provided by the invention is as follows:
the invention also provides a preparation method of the oligosaccharide breast-emulsified infant formula, which comprises the following steps:
(1) dissolving the desalted whey powder, the concentrated whey protein powder, the lactose, the taurine, the choline and the master emulsification oligosaccharide composition in the pretreated raw milk, and uniformly mixing to obtain a mixed solution A;
(2) adding anhydrous cream and vegetable oil into the mixed solution A obtained in the step (1), and uniformly mixing to obtain mixed solution B;
(3) respectively dissolving the compound mineral substance and the compound vitamin in water to respectively obtain a mixed solution C and a mixed solution D;
(4) adding the mixed solution C and the mixed solution D obtained in the step (3) into the mixed solution B obtained in the step (2), uniformly mixing, and cooling to obtain a mixed solution E;
(5) dissolving buffer salt in water to obtain a mixed solution F;
(6) slowly adding the mixed solution F obtained in the step (5) into the mixed solution E obtained in the step (4), uniformly stirring and cooling to obtain a mixed solution G;
(7) and (4) adjusting the dry matter concentration of the mixed liquor G obtained in the step (6) to 12.50-14.50%, homogenizing and sterilizing to obtain the product.
In order to retain the activity of nutrient substances in the formula milk and the edible safety of the formula milk in a shelf life to the maximum extent, in the step (1), the temperature of raw milk is 35-50 ℃, the adopted equipment during dissolution is a vacuum mixer, and the raw milk is circularly mixed for 10-30 minutes after the raw milk is added; the raw milk may also be a mixture of raw milk and water.
The pretreatment is the standardization, homogenization and sterilization treatment of raw milk.
In the step (2), the temperature of the anhydrous cream and the vegetable oil is 35-55 ℃, and the mixing time is 10-20 min.
In the step (3), the temperature of water is 15-45 ℃, and the concentration of the composite mineral substance after being dissolved in the water is 0.6-3.0% (w/w).
In the step (4), cooling to 5-30 ℃;
in the step (5), the concentration of the buffer salt dissolved in water is 0.1-0.5% (w/w).
In the step (6), cooling to 5-30 ℃.
In the step (7), the mixed solution G is fed into an evaporator, and water is added or evaporated to obtain the liquid milk solution with the dry matter content of 12.50-14.50% (w/w).
The homogenization is carried out for the second time, the pressure of the first time of homogenization is 15-19 MPa, the pressure of the second time of homogenization is 1-5 MPa, and the temperature of the two times of homogenization is 55-60 ℃.
And (4) performing ultrahigh-temperature instantaneous sterilization at the sterilization temperature of 130-134 ℃ for 2-5 seconds.
The main technical indexes of the infant formula prepared by the invention are shown in the following table:
TABLE 1 technical indices of formula milk
Compared with the prior art, the invention has the advantages that:
(1) the infant formula milk is a liquid system infant formula food, is simplified in process without spray drying, and has the characteristics of better retention of nutrient components and convenience in drinking compared with common infant formula powder;
(2) the infant formula milk provided by the invention is unstable due to the liquid system containing various nutrient substances, and is easy to have the phenomena of fat floating, protein precipitation, color change and the like, and after the buffer salt system is added, the problems are solved, and the stability of the product quality is ensured;
(3) the content of oligosaccharide contained in the infant formula milk is simulated according to the carbon chain distribution of the oligosaccharide in breast milk, so that the carbohydrate is more similar to the breast milk, and the infant formula milk is more suitable for infants.
The reagents and starting materials used in the present invention are commercially available.
On the basis of the common knowledge in the field, the preferred conditions can be combined randomly to obtain the preferred embodiments of the invention. On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention. On the basis of the common knowledge in the field, the preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers.
The percentages stated in the following examples are, unless otherwise specified, the same as those stated in the summary of the invention.
The following examples illustrate the source of each raw material:
raw milk: dairy products second plant, Guangming Dairy products GmbH.
Desalted whey powder, concentrated whey protein: virio (shanghai) food trade limited.
Lactose: virio (shanghai) food trade limited.
Anhydrous cream: new zealand dairy raw materials (shanghai) limited.
Blending vegetable oil: jiali grain and oil (Yingkou) Co., Ltd.
Vitamin complex: shanghai Yangchi food science and technology, Inc.
Compound mineral substance: shanghai Yangchi food science and technology, Inc.
Strong calcium oxide: food grade, Anhufeng original biochemistry corporation.
Citric acid: anhufeng original biochemistry, Inc., citric acid monohydrate, food grade, derived from corn starch.
Choline: the medicine is food grade Choline chloride.
Taurine: food grade, Yongan pharmaceutical industry, Ministry of the Yangxiang province.
GOS: shanghai New Shell Biotech, Inc.
FOS: shanghai Gexin health technology, Inc.
2' -fucosyllactose: basf (china) ltd.
Example 1
1. An oligosaccharide raw material formula for a breast milk formula (see table 2) was prepared to give an infant formula with a dry matter concentration of 13.5%.
Table 2 raw material recipe of example 1
Table 3 multivitamin composition of example 1
| Names of vitamins | Content (wt.) |
| Vitamin A | 5040～7560IU/g |
| Vitamin D3 | 1150～1730IU/g |
| Vitamin E | 24.6～37.0mg/g |
| Vitamin K1 | 120～170μg/g |
| Vitamin B1 | 2592～3888μg/g |
| Vitamin B2 | 2745～4118μg/g |
| Nicotinic acid | 22000～33000μg/g |
| Pantothenic acid | 7304～10956μg/g |
| Vitamin B6 | 1070～1605μg/g |
| Vitamin B12 | 7.3～10.9μg/g |
| Folic acid | 303.7～455.5μg/g |
| Biotin | 37.0～55.3μg/g |
| Vitamin C | 520.4～777.9mg/g |
Table 4 multivitamin composition of example 1
| Name of mineral element | Content (wt.) |
| Calcium carbonate | 120.0mg/g |
| Iron | 8.4～12.6mg/g |
| Manganese oxide | 50.42～120.10μg/g |
| Copper (Cu) | 672～1008μg/g |
| Zinc | 8.4～12.6mg/g |
| Selenium | 43.1～64.7μg/g |
| Iodine | 167～250μg/g |
| Chlorine | 152～228mg/g |
2. A method for preparing oligosaccharide breast milk infant formula comprises:
(1) dissolving the desalted whey powder, the master emulsified oligosaccharide composition, the choline and the taurine in the pretreated raw milk at 45 ℃ by a vacuum mixer, and circularly mixing for 15 minutes after the addition is finished;
(2) heating anhydrous cream and soybean oil to 45 ℃, adding the anhydrous cream and the soybean oil into the mixed solution obtained in the step (1), and stirring for 15 minutes to mix uniformly;
(3) respectively dissolving vitamin complex and compound mineral in deionized water at 30 deg.C to obtain a solution with concentration of 0.6% (w/w);
(4) adding the solution obtained in the step (3) into the mixed solution obtained in the step (2), uniformly mixing, and further cooling the obtained mixed solution to 5 ℃;
(5) dissolving citric acid and calcium hydroxide in deionized water at 5 deg.C to obtain a solution with a concentration of 0.1% (w/w);
(6) slowly adding the solution obtained in the step (5) into the mixed solution obtained in the step (4), and stirring and uniformly mixing the solution;
(7) the mixed liquor obtained in the step (6) is made up to a certain quantity by water, and the dry matter concentration of the mixed liquor is 14.5 percent (w/w);
(8) carrying out secondary homogenization on the mixed solution obtained in the step (7), wherein the first homogenization pressure is 15MPa, the second homogenization pressure is 5MPa, and the homogenization temperature is 55 ℃;
(9) and (4) carrying out ultrahigh-temperature instant sterilization on the mixed solution obtained in the step (8), wherein the sterilization temperature is 130 ℃, and the sterilization time is 5 seconds.
The product index test results are as follows (per 100ml of this infant formula):
TABLE 5 indices of example 1
Example 2
1. An oligosaccharide breast milk formula (see Table 6) was prepared to produce an infant formula with a dry matter concentration of 12.5%.
Table 6 stock formulation for example 2
The specific composition of the vitamin complex and the mineral complex is the same as in example 1.
2. A method for preparing oligosaccharide breast milk infant formula comprises:
(1) dissolving the desalted whey powder, the master emulsified oligosaccharide composition, the choline and the taurine in the pretreated raw milk at 50 ℃ by a vacuum mixer, and circularly mixing for 10 minutes after the addition is finished;
(2) heating anhydrous cream and soybean oil to 55 ℃, adding the anhydrous cream and the soybean oil into the mixed solution obtained in the step (1), and stirring for 10 minutes to mix uniformly;
(3) respectively dissolving vitamin complex and compound mineral in deionized water at 30 deg.C to obtain a solution with concentration of 1.5% (w/w);
(4) adding the solution obtained in the step (3) into the mixed solution obtained in the step (2), uniformly mixing, and further cooling the obtained mixed solution to 15 ℃;
(5) dissolving citric acid and calcium hydroxide in deionized water at 15 deg.C to obtain a solution with a concentration of 0.25% (w/w);
(6) slowly adding the solution obtained in the step (5) into the mixed solution obtained in the step (4), and stirring and uniformly mixing the solution;
(7) the mixed liquor obtained in the step (6) is made up to a certain quantity by water, and the dry matter concentration of the mixed liquor is 12.5 percent (w/w);
(8) carrying out secondary homogenization on the mixed solution obtained in the step (7), wherein the first homogenization pressure is 19MPa, the second homogenization pressure is 1MPa, and the homogenization temperature is 60 ℃;
(9) and (4) carrying out ultrahigh-temperature instant sterilization on the mixed solution obtained in the step (8), wherein the sterilization temperature is 134 ℃, and the sterilization time is 2 seconds.
The product index test results are as follows (per 100ml of this infant formula):
TABLE 7 indices of example 2
Example 3
1. An oligosaccharide breast milk formula (see table 8) was prepared to produce an infant formula with a dry matter concentration of 14.5%.
Table 8 raw material recipe for example 3
The specific composition of the vitamin complex and the mineral complex is the same as in example 1.
2. An infant formula is prepared according to the above formula by the following method:
(1) dissolving desalted whey powder, concentrated whey protein powder, a master emulsified oligosaccharide composition, choline and taurine in pretreated raw milk at 35 ℃ by a vacuum mixer, and circularly mixing for 30 minutes after the addition is finished;
(2) heating anhydrous cream and soybean oil to 35 ℃, adding the anhydrous cream and the soybean oil into the mixed solution obtained in the step (1), and stirring for 20 minutes to mix uniformly;
(3) respectively dissolving vitamin complex and compound mineral in deionized water at 30 deg.C to obtain 3% (w/w);
(4) adding the solution obtained in the step (3) into the mixed solution obtained in the step (2), uniformly mixing, and further cooling the obtained mixed solution to 30 ℃;
(5) dissolving citric acid and calcium hydroxide in deionized water at 30 deg.C to obtain a solution with a concentration of 0.5% (w/w);
(6) slowly adding the solution obtained in the step (5) into the mixed solution obtained in the step (4), and stirring and uniformly mixing the solution;
(7) the mixed liquor obtained in the step (6) is made up to a certain quantity by water, and the dry matter concentration of the mixed liquor is 14.5 percent (w/w);
(8) carrying out secondary homogenization on the mixed solution obtained in the step (7), wherein the first homogenization pressure is 18MPa, the second homogenization pressure is 3MPa, and the homogenization temperature is 60 ℃;
(9) and (4) carrying out ultrahigh-temperature instant sterilization on the mixed solution obtained in the step (8), wherein the sterilization temperature is 134 ℃, and the sterilization time is 2 seconds.
The product index test results are as follows (per 100ml of this infant formula):
TABLE 9 indices for example 3
Example 4
1. An oligosaccharide breast milk formula (see Table 10) was prepared to produce an infant formula with a dry matter concentration of 14.5%.
Table 10 stock formulation for example 4
The specific composition of the vitamin complex and the mineral complex is the same as in example 1.
2. An infant formula is prepared according to the above formula by the following method:
(1) dissolving the desalted whey powder, the master emulsified oligosaccharide composition, the choline and the taurine in the raw milk with the temperature of 35 ℃ through a vacuum mixer, and circularly mixing for 30 minutes after the raw milk is added;
(2) heating anhydrous cream and soybean oil to 35 ℃, adding the anhydrous cream and the soybean oil into the mixed solution obtained in the step (1), and stirring for 20 minutes to mix uniformly;
(3) respectively dissolving vitamin complex and compound mineral in deionized water at 30 deg.C to obtain a solution with concentration of 1% (w/w);
(4) adding the solution obtained in the step (3) into the mixed solution obtained in the step (2), uniformly mixing, and further cooling the obtained mixed solution to 20 ℃;
(5) dissolving citric acid and calcium hydroxide in deionized water at 20 deg.C to obtain a solution with a concentration of 0.5% (w/w);
(6) slowly adding the solution obtained in the step (5) into the mixed solution obtained in the step (4), and stirring and uniformly mixing the solution;
(7) the mixed liquor obtained in the step (6) is made up to a certain quantity by water, and the dry matter concentration of the mixed liquor is 14.5 percent (w/w);
(8) carrying out secondary homogenization on the mixed solution obtained in the step (7), wherein the first homogenization pressure is 18MPa, the second homogenization pressure is 5MPa, and the homogenization temperature is 55 ℃;
(9) and (4) carrying out ultrahigh-temperature instant sterilization on the mixed solution obtained in the step (8), wherein the sterilization temperature is 130 ℃, and the sterilization time is 5 seconds.
The product index test results are as follows (per 100ml of this infant formula):
TABLE 11 indices of example 4
Example 5
1. An oligosaccharide breast milk formula (see table 12) was prepared with an oligosaccharide breast milk formula dry matter concentration of 13.5%.
Table 12 raw material recipe for example 5
2. An infant formula is prepared according to the above formula by the following method:
(1) dissolving desalted whey powder, concentrated whey protein powder, lactose, a master emulsified oligosaccharide composition, choline and taurine in pretreated raw milk at 45 ℃ by a vacuum mixer, and circularly mixing for 15 minutes after the addition is finished;
(2) heating anhydrous cream and soybean oil to 45 ℃, adding the anhydrous cream and the soybean oil into the mixed solution obtained in the step (1), and stirring for 15 minutes to mix uniformly;
(3) respectively dissolving vitamin complex and compound mineral in deionized water at 30 deg.C to obtain a solution with concentration of 0.6% (w/w);
(4) adding the solution obtained in the step (3) into the mixed solution obtained in the step (2), uniformly mixing, and further cooling the obtained mixed solution to 5 ℃;
(5) dissolving citric acid and calcium hydroxide in deionized water at 5 deg.C to obtain a solution with a concentration of 0.1% (w/w);
(6) slowly adding the solution obtained in the step (5) into the mixed solution obtained in the step (4), and stirring and uniformly mixing the solution;
(7) the mixed liquor obtained in the step (6) is made up to a certain quantity by water, and the dry matter concentration of the mixed liquor is 13.5 percent (w/w);
(8) carrying out secondary homogenization on the mixed solution obtained in the step (7), wherein the first homogenization pressure is 15MPa, the second homogenization pressure is 5MPa, and the homogenization temperature is 55 ℃;
(9) and (4) carrying out ultrahigh-temperature instant sterilization on the mixed solution obtained in the step (8), wherein the sterilization temperature is 130 ℃, and the sterilization time is 5 seconds.
The product index test results are as follows (per 100ml of this infant formula):
TABLE 13 indices of example 5
Effects of the embodiment
Taking the oligosaccharide combinations selected in examples 1-5 as examples, respectively influencing the growth of the oligosaccharide combination bifidobacterium and the lactobacillus fermentum CECT5716, respectively inoculating the two strains in MRS culture media, respectively, incubating at 37 ℃ for 36h to reach logarithm, and respectively taking 100ul of the semi-homemade MRS culture media without carbohydrate sources. Anaerobic culture at 37 deg.C for 48 h. Optical density measurements were performed every 8h at a wavelength of 600nm using a spectrophotometer. The OD values were recorded and the results of the experiment are shown in FIGS. 1-2.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (10)
1. An oligosaccharide breast-emulsified infant formula is characterized by comprising the following raw materials in percentage by mass: 12.9-37.0% of raw milk, 2.0-8.88% of desalted whey powder, 0-2.08% of concentrated whey protein powder, 0.9-1.78% of anhydrous cream, 1.0-1.78% of blend vegetable oil, 0-25.6% of lactose, 0.48-1.0% of mother emulsified oligosaccharide composition, 0.045% of compound vitamin, 0.045% of compound mineral substance, 0.033-0.042% of buffer salt, 0.007-0.02% of choline, 0.006-0.015% of taurine and water for supplementing to 100%.
2. The oligosaccharide breast-emulsified infant formula according to claim 1 wherein the breast-emulsified oligosaccharide composition comprises: 2' -fucosyllactose, fructo-oligosaccharide, and galacto-oligosaccharide.
3. The oligosaccharide breast milk formula according to claim 1, wherein the buffer salts comprise citric acid and calcium hydroxide.
4. The oligosaccharide breast-emulsified infant formula of claim 1, wherein the blended vegetable oil is a blended raw material of soybean oil, corn oil and rapeseed oil which are mixed uniformly.
5. The oligosaccharide breast-emulsified infant formula according to claim 4, wherein the vitamin complex is a compound raw material obtained by uniformly mixing a plurality of vitamin monomers;
the compound mineral substance is a compound raw material formed by uniformly mixing a plurality of mineral substance monomers.
6. A process for the preparation of an oligosaccharide breast milk formula according to any one of claims 1 to 5, comprising the steps of:
(1) dissolving the desalted whey powder, the concentrated whey protein powder, the lactose, the taurine, the choline and the master emulsification oligosaccharide composition in the pretreated raw milk, and uniformly mixing to obtain a mixed solution A;
(2) adding anhydrous cream and blend vegetable oil into the mixed solution A obtained in the step (1), and uniformly mixing to obtain mixed solution B;
(3) respectively dissolving the compound mineral substance and the compound vitamin in water to respectively obtain a mixed solution C and a mixed solution D;
(4) adding the mixed solution C and the mixed solution D obtained in the step (3) into the mixed solution B obtained in the step (2), uniformly mixing, and cooling to obtain a mixed solution E;
(5) dissolving buffer salt in water to obtain a mixed solution F;
(6) slowly adding the mixed solution F obtained in the step (5) into the mixed solution E obtained in the step (4), uniformly stirring and cooling to obtain a mixed solution G;
(7) and (4) adjusting the dry matter concentration of the mixed liquor G obtained in the step (6) to 12.50-14.50%, homogenizing and sterilizing to obtain the product.
7. The method for preparing oligosaccharide breast-emulsified infant formula according to claim 6, wherein in the step (1), the temperature of raw milk is 35-50 ℃, and the mixing time is 10-30 min;
the pretreatment is the standardization, homogenization and sterilization treatment of raw milk.
8. The oligosaccharide breast-emulsified infant formula of claim 6, wherein in the step (2), the temperature of the anhydrous butter and the blended vegetable oil is 35-55 ℃, and the mixing time is 10-20 min;
in the step (3), the temperature of water is 15-45 ℃, and the concentration of the composite mineral substance after being dissolved in the water is 0.6-3.0%.
9. The method for preparing an oligosaccharide breast milk formula according to claim 6, wherein in step (4), the cooling is performed to 5-30 ℃;
in the step (5), the concentration of the buffer salt dissolved in water is 0.1-0.5%;
in the step (6), cooling to 5-30 ℃.
10. The method for preparing an oligosaccharide breast milk formula according to claim 6, wherein in step (7), the homogenization is a second homogenization, the first homogenization pressure is 15-19 MPa, the second homogenization pressure is 1-5 MPa, and the temperature of the two homogenization is 55-60 ℃.
And (4) performing ultrahigh-temperature instantaneous sterilization at the sterilization temperature of 130-134 ℃ for 2-5 seconds.
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