CN112704737B - A coronary artery endothelial cell angiogenesis promoter - Google Patents
A coronary artery endothelial cell angiogenesis promoter Download PDFInfo
- Publication number
- CN112704737B CN112704737B CN202110052771.5A CN202110052771A CN112704737B CN 112704737 B CN112704737 B CN 112704737B CN 202110052771 A CN202110052771 A CN 202110052771A CN 112704737 B CN112704737 B CN 112704737B
- Authority
- CN
- China
- Prior art keywords
- sirna
- coronary artery
- cells
- seq
- endothelial cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/713—Double-stranded nucleic acids or oligonucleotides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Microbiology (AREA)
- Cardiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Plant Pathology (AREA)
- Heart & Thoracic Surgery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention provides a coronary artery endothelial cell angiogenesis promoter, belonging to the technical field of cardiac medicine. Experiments show that the down-regulation of RP11-71G12.1 can effectively promote the proliferation and angiogenesis of endothelial cells of coronary arteries, so that the siRNA of RP11-71G12.1 can be used for preparing a medicament for treating ischemic heart disease, and a new treatment direction is provided for treating ischemic heart disease.
Description
Technical Field
The invention belongs to the technical field of cardiac medicine, and particularly relates to a coronary artery endothelial cell angiogenesis promoter.
Background
Ischemic heart disease is also called coronary heart disease, and refers to heart disease caused by myocardial ischemia, hypoxia or necrosis due to stenosis or obstruction of an organ caused by coronary atherosclerosis, and includes asymptomatic myocardial ischemia, angina pectoris, myocardial infarction, ischemic heart failure and cardiac arrest. With the increasing living standard, ischemic heart disease has become the leading cause of death in the world. Patients with ischemic heart disease are mainly supplemented and compensated by the establishment of collateral circulation in the heart. In the case of acute vascular occlusion in a patient, treatment may be provided by in situ enlargement or prolonged growth of the vessel and dilation of the lumen. Whereas, when a patient is chronically occluded in the coronary arteries, treatment may be effected in a manner that promotes the production of capillaries by angiogenic factors.
Although. At present, a plurality of medicines for clinically treating the ischemic heart disease exist, but the pathological mechanism and the physiological mechanism of the ischemic heart disease are very complex, so the effect of single-target-point medicine treatment is not ideal. In this case, angiogenesis is a new and potentially promising research direction. The therapeutic angiogenesis can effectively promote the growth of blood vessels around ischemic cardiac muscle, establish collateral circulation and improve the symptoms of ischemia and anoxia of patients. Therefore, studying the angiogenesis of coronary arteries helps to further treat ischemic heart disease.
Disclosure of Invention
The invention aims to provide a coronary artery endothelial cell angiogenesis promoter.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides application of an RP11-71G12.1 gene as a target molecule in promoting angiogenesis, wherein the External Transcript ID of the RP11-71G12.1 gene is ENST00000457239.1, and the sequence of ENST00000457239.1 is shown in SEQ ID NO. 1.
In addition, the invention provides siRNA for treating ischemic heart disease, wherein the siRNA is siRNA of gene RP11-71G12.1, the sense strand sequence of the siRNA is shown as SEQ ID NO.2, and the antisense strand sequence of the siRNA is shown as SEQ ID NO. 3.
In addition, the invention provides application of an inhibitor of the gene RP11-71G12.1 in preparing a medicament for treating ischemic heart disease.
Preferably, the inhibitor of the gene RP11-71G12.1 is siRNA of RP11-71G12.1, the sequence of the sense strand of the siRNA is shown as SEQ ID NO.2, and the sequence of the antisense strand of the siRNA is shown as SEQ ID NO. 3.
In addition, the invention provides application of an inhibitor of the gene RP11-71G12.1 in preparing a human coronary artery endothelial cell proliferation promoter.
Preferably, the inhibitor of the gene RP11-71G12.1 is siRNA of RP11-71G12.1, the sequence of the sense strand of the siRNA is shown as SEQ ID NO.2, and the sequence of the antisense strand of the siRNA is shown as SEQ ID NO. 3.
In addition, the application of the promoter of the gene RP11-71G12.1 in preparing the human coronary artery endothelial cell angiogenesis promoter.
Preferably, the inhibitor of the gene RP11-71G12.1 is siRNA of RP11-71G12.1, the sequence of the sense strand of the siRNA is shown as SEQ ID NO.2, and the sequence of the antisense strand of the siRNA is shown as SEQ ID NO. 3.
The invention has the beneficial effects that:
the invention provides siRNA capable of effectively inhibiting gene RP11-71G 12.1; secondly, the invention discovers that the down-regulation of RP11-71G12.1 can effectively promote the proliferation and angiogenesis of endothelial cells of coronary arteries for the first time, so that the siRNA of RP11-71G12.1 can be used for preparing the medicine for treating the ischemic heart disease, and a new treatment direction is provided for treating the ischemic heart disease.
Drawings
FIG. 1 is a knock-down effect assay of si-RP11-71G 12.1;
FIG. 2 downregulation of the effect of si-RP11-71G12.1 on cell proliferation of HCAEC cells;
FIG. 3 downregulation of the effect of si-RP11-71G12.1 on HCAEC cell vascularization;
FIG. 4 Down-regulates the effect of si-RP11-71G12.1 on vascular endothelial growth factor A expression.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.
Example 1
Design and verification of siRNA of RP11-71G12.1
(A) Design of RP11-71G12.1 siRNA
The siRNA designed according to the RP11-71G12.1 gene sequence is as follows:
sense strand: 5' -UUCUUCUUCUUCUUCUUCCCA-3, SEQ ID NO. 2;
antisense strand: 5'-GGAAGAAGAAGAAGAAGAAAG-3', SEQ ID NO. 3;
(B) verification of the inhibitory Effect of si-RP11-71G12.1
1. Extraction of RNA from human coronary endothelial cells (HCAEC cells) transfected with si-NC and si-RP11-71G12.1
(1) HCAEC cells were seeded in cell culture plates and si-NC and si-RP11-71G12.1 were transfected according to lip2000 instructions, with 3 replicates per set;
(2) after transfection for 48h, removing the culture medium, washing the cells for 3 times by using PBS, adding 1ml of Trizol into each hole, blowing and uniformly mixing, and standing for 5min at room temperature;
(3) transferring the cells into an EP tube, adding 200 mu l of chloroform, uniformly mixing, and standing at room temperature for 5 min;
(4) adjusting the parameters of the centrifuge to 4 ℃, 12000rpm/min, and centrifuging for 15 min;
(5) after centrifugation, carefully suck the upper aqueous phase into a new EP tube;
(6) adding isovoluminal precooled isopropanol, uniformly mixing, standing at room temperature for 10min, and centrifuging at 4 ℃ at 12000rpm/min for 10 min;
(7) discarding the supernatant, and adding 75% ethanol prepared from DEPC water to wash and precipitate;
(8) adjusting the parameters of the centrifuge to 4 ℃, 8000rpm/min, and centrifuging for 5 min;
(9) the supernatant was discarded, dried in a clean bench until the ethanol evaporated completely, and 30. mu.l of DEPC water was added.
2. Reverse transcription to obtain cDNA
(1) Removal of genomic DNA
The reaction system is as follows:
| reagent | Adding amount of |
| 5×gDNA Eraser Buffer | 2.0 μl |
| gDNA Eraser | 1μl |
| Total RNA | 1μg |
| RNase Free dH2O | up to 10 μl |
The reaction conditions were as follows: stored at 42 ℃ for 2 minutes and 4 ℃.
(2) Reverse transcription reaction
The reaction system is as follows:
| reagent | Adding amount of |
| Reaction solution of step (1) | 10μl |
| PrimeScript RT Enzyme Mix I | 1.0μl |
| RT Primer Mix | 1.0 μl |
| 5×PrimeScript Buffer 2 | 4.0 μl |
| RNase Free dH2O | 4.0 |
| Total | |
| 20 μl |
The reaction conditions were as follows: 15min at 37 ℃; 5s at 85 ℃; storing at 4 ℃.
3. Detecting the inhibitory effect by RT-PCR
(1) An RP11-71G12.1 primer is designed, and the primer sequence is as follows:
an upstream primer: 5'-AGCCTGTGTCAAGGTTCATGT-3', SEQ ID NO. 4;
a downstream primer: 5'-TCATACCATGCATTGCTGTTCA-3', SEQ ID NO. 5;
(2) and performing PCR reaction by taking GAPDH as an internal reference, wherein the PCR reaction system is as follows:
| reagent | Adding amount of |
| cDNA | 1μl |
| TaKaRa Ex Taq | 0.5μl |
| 10×Ex Taq buffer | 5μl |
| dNTP mixture | 4μl |
| Primer F | 2μl |
| Primer R | 2μl |
| ddH2O | 35.5μl |
PCR reaction procedure:
Step 1 94℃ 3min
Step 2 94℃ 1min， 60℃ 1min， 72℃ 1min， 35 Cycles
Step 3 72℃ 5 min
Step 4 4℃ forever；
(3) after the PCR is finished, the inhibition effect is detected by electrophoresis, and the result is shown in FIG. 1, and it can be seen from the figure that the expression of RP11-71G12.1 can be effectively inhibited by down-regulating RP11-71G 12.1.
Example 2
Down-regulation of RP11-71G12.1 promotes proliferation of endothelial cells of human coronary artery
(1) HCAEC cells were seeded in 96-well plates and transfected individually with si-NC and si-RP11-71G12.1, with 3 replicates per group;
(2) after 48h of transfection, the proliferation of the cells was measured and counted using CCK-8, and the statistics are shown in FIG. 2.
The results show that the down-regulation of RP11-71G12.1 can effectively promote the proliferation of human coronary endothelial cells (the relative proliferation rate of the si-RP11-71G12.1 group cells is 1.381 +/-0.046, which is obviously higher than that of the si-NC group, and the difference has statistical significance).
Example 3
Down-regulation of RP11-71G12.1 promotes angiogenesis of human coronary endothelial cells
(1) Adding 50 mul of Matrigel glue into a 96-well plate, slightly shaking the 96-well plate to enable the Matrigel glue to be paved in the well, and placing for 2 hours at 37 ℃;
(2) inoculating HCAEC cells into a 6-well plate, transfecting si-NC and si-RP11-71G12.1 respectively, and collecting the cells after transfecting for 48 hours;
(3) the cells were prepared as single cell suspensions and added to a 96-well plate supplemented with Matrigel gel at 1X 10 per well4(ii) individual cells;
(4) the 96-well plate was placed in a cell incubator, and after 24 hours of incubation, the vessel lumen formation was observed under an inverted microscope, and the results of the pictures are shown in FIG. 3.
The results show that the down-regulation of RP11-71G12.1 can obviously increase the number of HCARC cell lumen formation lumens, and the down-regulation of RP11-71G12.1 can effectively promote the angiogenesis of human coronary artery endothelial cells.
Example 4
Down-regulation of protein expression of RP11-71G12.1 promotion Vascular Endothelial Growth Factor A (VEGFA)
(1) HCAEC cells were seeded in 6-well plates, transfected with si-NC and si-RP11-71G12.1, triplicates per set;
(2) after transfection for 48h, the cells were washed with PBS, 100. mu.l of RIPA cell lysate was added to each well, and the cells were scraped off using a cell scraper and transferred to an EP tube;
(3) after cell lysis for 30min, placing in a centrifuge at 1200rpm/min, centrifuging for 10min, and taking the supernatant to a new EP tube;
(4) quantifying protein by using a BCA method, adding 5 Xloading buffer solution, and boiling for 5min with boiling water to obtain a protein sample;
(5) preparing 5% of upper layer glue and 12% of lower layer glue, assembling an electrophoresis tank, adding 20 mug of protein sample and a Marker indicator, and adding electrophoresis liquid for electrophoresis;
(6) after electrophoresis is finished, assembling an electric rotating clamp, and rotating the membrane for 1.5 hours at 200 mA;
(7) after the electrotransformation is finished, transferring the PVDF membrane into 5% skimmed milk powder, and sealing for 1h at room temperature;
(8) after washing the membrane, incubating VEGFA and GAPDH primary antibody overnight at 4 ℃;
(9) after washing the membrane, incubating corresponding secondary antibody, and incubating for 1h in a shaking table at room temperature;
(10) in the dark, development exposure was performed, and the experimental results are shown in fig. 4.
The results show that the down regulation of RP11-71G12.1 promotes the protein expression of vascular endothelial growth factor A, and further prove that the down regulation of RP11-71G12.1 can effectively promote the angiogenesis of human coronary artery endothelial cells.
The technical features of the present invention which are not described in the above embodiments may be implemented by or using the prior art, and are not described herein again, of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and variations, modifications, additions or substitutions which may be made by those skilled in the art within the spirit and scope of the present invention should also fall within the protection scope of the present invention.
Sequence listing
<110> ninth people hospital in Qingdao city
<120> an agent for promoting angiogenesis of coronary endothelial cells
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 471
<212> DNA
<213> Human source (Human)
<400> 1
cttaccttgg ggaagcctgt gtcaaggttc atgtagactg gacacggctc tccttcgcga 60
gatattcttg gagtcattgc atttgtgctg ttgatcctgg gagctgggct atacctctgg 120
aagaaatgtg ggaagaagaa gaagaagaaa gatgaagagg aagaagagga agaggagcag 180
aaggaggagg aggagcggga ggagacagga ataggcaggt tgcatttctc catcgaggcc 240
cagatatctc ccctccccca ctagaatctg ggttctcttt ctcctgaaag aatgtcattg 300
tttagctata tgaataaccc tagatatagc atcgagttaa catgtccaag atatttgtta 360
ctggaaaaaa ctgaacagca atgcatggta tgatttaatt tttgtaaaag tgtatagata 420
ggtagataga aagataagca cagaataaaa tctatactac atgacagatt a 471
<210> 2
<211> 21
<212> RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
uucuucuucu ucuucuuccc a 21
<210> 3
<211> 21
<212> RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
ggaagaagaa gaagaagaaa g 21
<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
agcctgtgtc aaggttcatg t 21
<210> 5
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
tcataccatg cattgctgtt ca 22
Claims (1)
1. The application of the inhibitor of the gene RP11-71G12.1 in preparing the human coronary artery endothelial cell proliferation accelerant is characterized in that the sequence of the gene RP11-71G12.1 is shown as SEQ ID NO.1, the inhibitor of the gene RP11-71G12.1 is siRNA of RP11-71G12.1, the sequence of the sense strand of the siRNA is shown as SEQ ID NO.2, and the antisense strand of the siRNA is shown as SEQ ID NO. 3.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110052771.5A CN112704737B (en) | 2021-01-15 | 2021-01-15 | A coronary artery endothelial cell angiogenesis promoter |
| CN202111173345.3A CN113768947B (en) | 2021-01-15 | 2021-01-15 | Application of gene inhibitor in preparation of ischemic heart disease treatment medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110052771.5A CN112704737B (en) | 2021-01-15 | 2021-01-15 | A coronary artery endothelial cell angiogenesis promoter |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111173345.3A Division CN113768947B (en) | 2021-01-15 | 2021-01-15 | Application of gene inhibitor in preparation of ischemic heart disease treatment medicine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112704737A CN112704737A (en) | 2021-04-27 |
| CN112704737B true CN112704737B (en) | 2021-11-16 |
Family
ID=75549140
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110052771.5A Active CN112704737B (en) | 2021-01-15 | 2021-01-15 | A coronary artery endothelial cell angiogenesis promoter |
| CN202111173345.3A Active CN113768947B (en) | 2021-01-15 | 2021-01-15 | Application of gene inhibitor in preparation of ischemic heart disease treatment medicine |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111173345.3A Active CN113768947B (en) | 2021-01-15 | 2021-01-15 | Application of gene inhibitor in preparation of ischemic heart disease treatment medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN112704737B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115804827B (en) * | 2022-03-18 | 2023-06-30 | 山东大学齐鲁医院(青岛) | Application of polypeptide in preparation of protective agent for reducing vascular endothelial function injury |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343724B (en) * | 2018-04-02 | 2021-10-12 | 北京大学 | Method for screening and identifying functional lncRNA |
| CN108815526A (en) * | 2018-06-19 | 2018-11-16 | 上海大学 | β 3-endonexin protein factor can be used as the application for the target molecules that regulation new vessels are formed |
-
2021
- 2021-01-15 CN CN202110052771.5A patent/CN112704737B/en active Active
- 2021-01-15 CN CN202111173345.3A patent/CN113768947B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112704737A (en) | 2021-04-27 |
| CN113768947A (en) | 2021-12-10 |
| CN113768947B (en) | 2023-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108179194B (en) | Tumor molecular marker circBIRC6, and inhibitor and application thereof | |
| CN110384800B (en) | Application of LncRNA XLOC _075168 in preparation of medicine for promoting angiogenesis | |
| Su et al. | miR‐30e‐3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr‐1 during Ischemia/Hypoxia | |
| CN112704737B (en) | A coronary artery endothelial cell angiogenesis promoter | |
| KR20110110058A (en) | Method for inhibiting senescence of adult stem cells using inhibition of mirna expression | |
| CN112852818A (en) | Circular RNA hsa _ circ _0001550 and application thereof | |
| EP3641833A1 (en) | Methods and compositions for controlling cardiac fibrosis and remodeling | |
| Li et al. | Upregulated miR‐206 aggravates deep vein thrombosis by regulating GJA1‐Mediated autophagy of endothelial progenitor cells | |
| CN108913695B (en) | Application of ZEB1 in human heart fibroblast | |
| CN115369077A (en) | MEFLC cell strain, and construction method and application thereof | |
| CN112656947B (en) | Endothelial cell injury inhibitor | |
| CN113234811A (en) | Application of CircRNA000338 and medicine thereof | |
| Guo et al. | MicroRNA‐384‐5p protects against cardiac hypertrophy via the ALPK3 signaling pathway | |
| CN113774061A (en) | Application of gene in preparation of NK cell activation drug | |
| CN114177298B (en) | Application of ADAR1 as medicine for treating pulmonary hypertension disease | |
| CN113069548B (en) | Reagent for cardiac revascularization | |
| CN109097358B (en) | Application of lncRNA in prevention or treatment of hypertension | |
| Li et al. | Engineered Circular RNA CircmiR‐29b Attenuates Muscle Atrophy by Sponging MiR‐29b | |
| CN111705061B (en) | Antisense nucleotide of piRNA-P1 and piRNA-P1 related to heart disease, application and medicament | |
| CN109706241B (en) | Medicine for treating dilated cardiomyopathy and screening and preparation method thereof | |
| CN102533982B (en) | The novelty teabag of people KLF8 gene in oncotherapy | |
| Zhangdi et al. | From phenomenon to essence: a newly involved lncRNA Kcnq1ot1 protective mechanism of bone marrow mesenchymal stromal cells in liver cirrhosis | |
| CN110433170B (en) | Application of cardiac pumping liquid miR-28-5p in heart diseases | |
| CN114748492B (en) | Medicine for treating atherosclerosis | |
| CN115094090B (en) | Cell modified by specific heparan sulfate modified enzyme gene, construction method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |