CN112690375B - Premix for preventing and treating white spot disease of marine fish and preparation method and application thereof - Google Patents
Premix for preventing and treating white spot disease of marine fish and preparation method and application thereof Download PDFInfo
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- CN112690375B CN112690375B CN202011566334.7A CN202011566334A CN112690375B CN 112690375 B CN112690375 B CN 112690375B CN 202011566334 A CN202011566334 A CN 202011566334A CN 112690375 B CN112690375 B CN 112690375B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention relates to a premix, in particular to a premix for preventing and treating white spot disease of marine fish, and a preparation method and application thereof. The premix for preventing and treating the ichthyophthiriasis of the marine fishes comprises the following components in parts by weight: 5-10 parts of eucalyptus oil, 10-15 parts of hesperidin, 25-35 parts of pine pollen, 15-25 parts of pseudo-ginseng powder, 10-20 parts of sodium humate and 10-20 parts of taurine. Compared with the prior art, the invention has the following beneficial effects: (1) the cryptocaryon irritans parasitism can be effectively prevented or reduced; (2) The anti-inflammatory and antibacterial effects can effectively reduce bacterial secondary infection and skin ulceration; (3) The growth of the marine fishes is promoted, the feed conversion rate is improved, and the feed coefficient is reduced; (4) The morbidity and mortality of the ichthyophthiriasis of the marine fishes in the stage of the ichthyophthiriasis are obviously reduced, and the breeding success rate and benefit are improved.
Description
Technical Field
The invention relates to a premix, in particular to a premix for preventing and treating white spot disease of marine fishes, and a preparation method and application thereof.
Background
China is the country with the largest aquaculture yield in the world and is one of a few countries with the aquaculture amount exceeding the fishing amount, wherein the aquaculture yield of seawater fishes exceeds 140 ten thousand tons (Chinese fishery statistics yearbook, 2018), the direct economic output value exceeds 500 hundred million, and the working population exceeds ten million. The seawater fish has delicious meat quality, is rich in amino acid and fatty acid (especially EPA and DHA), and is a high-quality protein source on the dining table of common people. Mariculture plays an important role in the countryside.
White spot disease is easy to occur in the marine fish culture process, and particularly, the disease is very popular when the water temperature is increased but is lower than 28 ℃ in spring and summer handover seasons. During disease attack, small white spots can be seen on the skin, fin-shaped rays, gills, oral cavities and the like of the body surfaces of the diseased fishes, and meanwhile, mucus is increased, the appetite is poor, the physique is thin and weak, the growth is blocked, and the death rate is high.
The etiology of ichthyophthiriasis is cryptocaryon irritans, which is a species of protozoa, oligohymenidae, hymenophorales, notched family, cryptocaryon, and is also called seawater ichthyophthiriasis because it is very similar to the ichthyophthiriasis, which is parasitic on freshwater fish.
Ichthyophthiriasis can occur in almost all mariculture species, is particularly serious in harm to large yellow croaker, golden pomfret, grouper and Pagruidae fishes, and is easy to cause death and aggravation due to secondary bacterial diseases because the body surfaces of the fishes are damaged and poor in resistance during the disease. The seawater cage culture is not easy to disinfect and use medicine, so the ichthyophthiriasis causes serious economic loss to the seawater culture. In the marine fish culture, how to effectively prevent and treat ichthyophthiriasis becomes a key point in the marine fish culture.
Disclosure of Invention
Aiming at the defects, the invention provides the premix for preventing and treating the ichthyophthiriasis of the marine fishes, and the preparation method and the application thereof, so that the morbidity and mortality of the ichthyophthiriasis of the marine fishes can be effectively reduced, and the growth speed of the marine fishes in the stage of the ichthyophthiriasis is increased.
The invention achieves the above purposes through the following scheme:
in a first aspect, the invention provides a premix for preventing and treating ichthyophthiriasis of marine fishes, which comprises the following components in parts by weight: 5-10 parts of eucalyptus oil, 10-15 parts of hesperidin, 25-35 parts of pine pollen, 15-25 parts of pseudo-ginseng powder, 10-20 parts of sodium humate and 10-20 parts of taurine.
In a preferred embodiment, the premix for preventing and treating the ichthyophthiriasis of the marine fishes comprises the following components in parts by weight: 10 parts of eucalyptus oil, 10 parts of hesperidin, 30 parts of pine pollen, 15 parts of pseudo-ginseng powder, 15 parts of sodium humate and 20 parts of taurine.
In a preferred embodiment, the premix for preventing and treating the ichthyophthiriasis of the marine fishes comprises the following components in parts by weight: 5 parts of eucalyptus oil, 15 parts of hesperidin, 25 parts of pine pollen, 20 parts of pseudo-ginseng powder, 20 parts of sodium humate and 15 parts of taurine.
In a preferred embodiment, the premix for preventing and treating the ichthyophthiriasis of the marine fishes comprises the following components in parts by weight: 8 parts of eucalyptus oil, 12 parts of hesperidin, 35 parts of pine pollen, 25 parts of pseudo-ginseng powder, 10 parts of sodium humate and 10 parts of taurine.
In the premix of the invention, the eucalyptus oil is obtained by steam distilling eucalyptus leaves and branches, has good antibacterial and bactericidal properties, and is commonly used in cleaning products for washing and nursing, and the eucalyptol contained in the eucalyptus oil has pungent smell, and can prevent parasites from biting and prevent skin diseases.
In the premix of the invention, hesperidin, also called hesperetin, exists in the wastes of citrus processing, such as pericarp and fruit sacs, has vitamin P-like efficacy, can maintain osmotic pressure, enhance the toughness of capillary vessels, prevent capillary rupture and bleeding, and also has certain antibacterial and anti-inflammatory effects.
In the premix of the invention, the pine pollen is dry pollen of masson pine, chinese pine or plants of the same genus, is a Chinese medicament, is warm in nature and sweet in taste, and has the effects of astringing to stop bleeding, eliminating dampness and healing sore. Pollen Pini contains abundant amino acids, natural vitamins and enzymes, and has effects of enhancing digestion function and stimulating appetite. Vitamins, rutin and flavonoids in the pine pollen can improve the activity of the heart and enhance the elasticity of the vessel wall. In addition, the pine pollen can enhance skin metabolism, prevent and treat skin erosion and has good treatment effect on skin diseases.
In the premix of the invention, the notoginseng powder is also named pseudo-ginseng powder, which is powder formed by beating the main rhizome of notoginseng. Has the functions of astringing to stop bleeding, eliminating blood stasis to promote tissue regeneration, eliminating swelling and relieving pain, and is one kind of traditional Chinese medicine material.
In the premix, the sodium humate is black amorphous particles, is nontoxic, odorless and non-corrosive, is very easy to dissolve in water, has the effects of resisting bacteria and viruses, diminishing inflammation and promoting wound healing, and is mainly used for astringing, stopping bleeding and stopping diarrhea and adsorbing intestinal inflammatory substances and toxins. In aquaculture, it also has water quality purifying, fertilizer supplying, chelating, antibacterial, and growth promoting effects.
In the premix of the invention, taurine, also called taurocholic acid, is an amino acid necessary for the growth and development of animals, and has the functions of enhancing appetite, promoting protein synthesis, regulating lipid metabolism, promoting growth and improving feed efficiency. Taurine can also promote lymphocyte proliferation, generate antibody, promote macrophage to generate interleukin-1, enhance phagocytic and bactericidal capacity of neutrophil, and improve the specific and nonspecific immunity of animals. In addition, taurine can protect nerve tissues and cardiac muscle cells damaged by hypoxia, improve the activity of the cardiac muscle cells, regulate the responsiveness of cardiovascular and cerebrovascular to hypoxia, and enhance the tolerance of organisms to hypoxia.
According to the invention, the hesperetin, the pine pollen, the pseudo-ginseng powder and the sodium humate are combined according to specific weight parts, and through the synergistic effect of the hesperetin, the pine pollen, the pseudo-ginseng powder and the sodium humate, the toughness and the convergence capability of capillary vessels of the seawater fish can be effectively increased, the body surface bleeding after the white spot disease occurs is reduced, the wound healing capability is enhanced, meanwhile, the pine pollen and the taurine have the functions of enhancing appetite and promoting digestion, the seawater fish likes to eat, the negative influence of eucalyptus oil on the eating is effectively counteracted, the eucalyptol contained in the eucalyptus oil has pungent smell, the parasite biting of parasites can be prevented, and the parasite expelling effect is achieved. The premix of the invention can effectively prevent or reduce the parasitism of cryptocaryon irritans.
In a second aspect, the invention provides a preparation method of the premix for the marine fish for preventing and treating the ichthyophthiriasis, which comprises the following steps:
(1) Mixing hesperidin, pollen pini, pseudo-ginseng powder, sodium humate and taurine uniformly to obtain a mixture;
(2) Micronizing the mixture;
(3) Adding eucalyptus oil, and mixing to obtain premix.
Preferably, the preparation method of the premix for marine fish for preventing and treating ichthyophthiriasis comprises the following steps:
(1) Evenly mixing hesperidin, pollen pini, pseudo-ginseng powder, sodium humate and taurine to obtain a mixture;
(2) Micronizing the mixture, and sieving with 100 mesh sieve;
(3) Adding eucalyptus oil, and mixing to obtain premix.
In a third aspect, the premix for the marine fish prepared by the preparation method is provided.
In a fourth aspect, the application of the premix for preventing and treating the ichthyophthiriasis of the marine fishes is provided, and the addition amount of the premix is 0.5-2% of the weight percentage of the compound feed for the marine fishes.
Preferably, the addition amount of the premix for preventing and treating the fish white spot disease of the marine fishes is 1 percent of the weight percentage of the compound feed for the marine fishes.
The application of the premix for preventing and treating the ichthyophthiriasis of the seawater fish is used for preventing the ichthyophthiriasis of the seawater fish, and/or treating the ichthyophthiriasis of the seawater fish, and/or promoting the growth speed of the seawater fish, and/or reducing the feed coefficient of a seawater fish feed.
Compared with the prior art, the invention has the following beneficial effects:
(1) Can effectively prevent or reduce the parasitism of cryptocaryon irritans;
(2) The anti-inflammatory and antibacterial effects are achieved, and the secondary bacterial infection and skin ulceration can be effectively reduced;
(3) The growth of the marine fishes is promoted, the feed conversion rate is improved, and the feed coefficient is reduced;
(4) The morbidity and mortality of the ichthyophthiriasis of the marine fishes in the stage of the ichthyophthiriasis are obviously reduced, and the breeding success rate and benefit are improved.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
The premix for preventing and treating the white spot disease of the marine fish comprises the following components in parts by weight: 10 parts of eucalyptus oil, 10 parts of hesperidin, 30 parts of pine pollen, 15 parts of pseudo-ginseng powder, 15 parts of sodium humate and 20 parts of taurine.
The use of the premix: 1 part of the prepared premix is uniformly mixed with 99 parts of common large yellow croaker compound feed raw materials, and then the mixture is subjected to puffing granulation to prepare the feed for feeding test large yellow croakers.
Example 2
The premix for preventing and treating the white spot disease of the marine fishes comprises the following components in parts by weight: 5 parts of eucalyptus oil, 15 parts of hesperidin, 25 parts of pine pollen, 20 parts of pseudo-ginseng powder, 20 parts of sodium humate and 15 parts of taurine.
The use of the premix: 1 part of the prepared premix and 99 parts of the raw materials of the common golden pomfret compound feed are mixed uniformly and then puffed and granulated, and the prepared feed is used for feeding test golden pomfret.
Example 3
The premix for preventing and treating the white spot disease of the marine fish comprises the following components in parts by weight: 8 parts of eucalyptus oil, 12 parts of hesperidin, 35 parts of pine pollen, 25 parts of pseudo-ginseng powder, 10 parts of sodium humate and 10 parts of taurine.
Comparative example 1
The common large yellow croaker puffed compound feed comprises the following components in parts by weight: 25 parts of Peru fish meal, 15 parts of domestic fish meal, 10 parts of American chicken bone meal, 5 parts of corn protein powder, 8 parts of soybean protein concentrate, 7 parts of peeled soybean meal, 18 parts of flour, 3.5 parts of fish oil, 3.5 parts of soybean oil, 2 parts of monocalcium phosphate, 2 parts of vitamin and mineral salt premix, 0.3 part of choline chloride, 0.2 part of lutein, 0.04 part of ethoxyquinoline (60%), 0.05 part of propionic acid (50%) and 0.41 part of fine bran.
The vitamin and mineral mixture contains the following ingredients per kg: vitamin A,5.4g; vitamin D 3 0.8; vitamin E,40; vitamin K 3 1.5; vitamin B 1 4, 4; riboflavin (80%), 10; pyridoxine, 3.5g; vitamin B 12 (1%), 1.5g; vitamin C (35%), 350g; calcium pantothenate, 30g; 30g of nicotinic acid; inositol, 80g; biotin (2%), 10g; folic acid, 3g；FeSO 4 ·H 2 O，10g；ZnSO 4 ·H 2 O，6g；CuSO 4 ·5H 2 O，1.5g；MnSO 4 ·H 2 O，6g；KI，8g；CoCl 2 ·6H 2 O(1％)，6g；Na 2 SeO 3 (1％)，2.5g；MgSO 4 ·7H 2 O,135g; 100g of NaCl; 120g of calcium propionate and 35.3g of cellulose.
Comparative example 2
The common golden pomfret puffed compound feed comprises the following components in parts by weight: 15 parts of Peru fish meal, 15 parts of domestic fish meal, 10 parts of American chicken bone meal, 3 parts of porcine blood globulin powder, 7 parts of corn protein powder, 5 parts of soybean protein concentrate, 10.5 parts of peeled soybean meal, 20 parts of flour, 5 parts of fish oil, 5 parts of soybean oil, 1.5 parts of monocalcium phosphate, 2 parts of vitamin and mineral salt premix, 0.5 part of choline chloride, 0.2 part of lutein, 0.04 part of ethoxyquinoline (60%), 0.05 part of propionic acid (50%), and 0.21 part of fine bran.
The vitamin and mineral mixture contains the following ingredients per kg: vitamin A,5.4g; vitamin D 3 0.8; vitamin E,40; vitamin K 3 1.5; vitamin B 1 4, a; riboflavin (80%), 10; pyridoxine, 3.5g; vitamin B 12 (1%), 1.5g; vitamin C (35%), 350g; calcium pantothenate, 30g; 30g of nicotinic acid; inositol, 80g; biotin (2%), 10g; folic acid, 3g; feSO 4 ·H 2 O，10g；ZnSO 4 ·H 2 O，6g；CuSO 4 ·5H 2 O，1.5g；MnSO 4 ·H 2 O，6g；KI，8g；CoCl 2 ·6H 2 O(1％)，6g；Na 2 SeO 3 (1％)，2.5g；MgSO 4 ·7H 2 O,135g; naCl,100g; 120g of calcium propionate and 35.3g of cellulose.
Effect comparative test 1
The culture test was carried out on the fish raft in the sea area of the Changhui island, xi nan province, xia county, nippon city, ningde, fujian province from 5/month 2/2018 to 27/month 27/2018. Selecting 25600 large yellow croakers with healthy appearance and uniform specification, respectively transferring the large yellow croakers into 8 test net cages (8 m multiplied by 4m multiplied by 6m, water depth: 5.6 m), randomly dividing each net cage into 2 groups, and feeding the large yellow croakers with 3 groups of the large yellow croakers to the feed of the example 1 (the feed prepared from 1 part of premix of the example 1 and 99 parts of feed of the comparative example 1) and the feed of the comparative example 1, wherein the feeding is performed at regular time of 5. And (4) recording the daily food intake, the number of dead fish (distinguishing the number of fish dead with white spots) and the weight of the dead fish of each net cage. The test period is 56 days, and the fish in each net cage is counted and weighed when the test is finished, and whether the fish suffers from white spot disease is observed and recorded. The test results are shown in Table 1.
Table 1:
| example 1 | Comparative example 1 | |
| Initial average amount (g) | 125.6±0.4 | 126.3±0.3 |
| Final average dose (g) | 220.9±2.1a | 203.6±3.2b |
| Percentage of increase (%) | 75.9±0.9a | 61.2±1.1b |
| Feeding amount (g) | 135.9±1.4a | 125.3±1.2b |
| Feed factor (weight of dead fish in calculation) | 1.43±0.02a | 1.62±0.02b |
| Total mortality (%) | 2.4±0.3a | 18.1±2.5b |
| White Point disease incidence (%) | 3.2±0.6a | 45.7±4.3b |
| White point disease mortality (%) | 1.0±0.2a | 16.6±2.8b |
Note: the difference is significant for different lower case letters after the same row data in the table (P < 0.05)
As can be seen from the data in Table 1, the food intake, terminal body weight and weight gain of the large yellow croakers in the group of example 1 are all significantly higher than those in the group of comparative example 1, and the feed coefficient, total mortality, ichthyophthiriasis morbidity and ichthyophthiriasis mortality of the group of example 1 are all significantly lower than those of comparative example 1.
The results show that the premix can obviously reduce the morbidity and mortality of the large yellow croaker in the stage of easy development of ichthyophthiriasis, and simultaneously can obviously improve the food intake and the long-term speed of the large yellow croaker and reduce the feed coefficient.
Effect comparison test 2
The breeding test was carried out on the fish raft in the seaport area of Yangjiang City, guangdong province, from 1 st in 2019 to 30 th in 2019 in 6 th month. Selecting 25600 tails of golden pomfret with healthy appearance and uniform specification, respectively transferring into 8 test net cages (6 m multiplied by 6m, the water depth: 5.6 m), randomly dividing 5000 tails of each net cage into 2 groups, repeating 3 groups, respectively feeding the feed of example 2 (the feed prepared from 1 part of premix of example 2 and 99 parts of feed of comparative example 2) and the feed of comparative example 2, and feeding at regular time of 500 and 18 parts per day for at least half an hour, so that the appearance of the pomfret is saturated. And (5) recording the daily food intake, the number of dead fishes (the number of fish died due to white spot disease is distinguished) and the weight of the dead fishes per net cage. The test period is 30 days, and the fish in each net cage is counted and weighed when the test is finished, and whether the fish suffers from white spot disease is observed and recorded. The test results are shown in Table 2.
Table 2:
note: the difference is significant for different lower case letters after the same row data in the table (P < 0.05)
As can be seen from the data in Table 2, the food intake, terminal body weight and weight gain of golden pomfret in the group of example 2 are all significantly higher than those in the group of comparative example 2, and the feed factor, total mortality, ichthyophthiriasis morbidity and ichthyophthiriasis mortality of the group of example 2 are all significantly lower than those of the group of comparative example 2.
The results show that the premix provided by the invention can be used for remarkably reducing the morbidity and mortality of golden pomfret at the stage of easily occurring white spot disease, and simultaneously can be used for remarkably improving the food intake and the growth rate of golden pomfret and reducing the feed coefficient.
Comparative Effect test example 3
To show that the additive components of the present invention have synergistic effects, 6 single-component-missing additives were prepared on the basis of example 1. The 1 st lack of eucalyptus oil, the 2 nd lack of hesperidin, the 3 rd lack of pine pollen, the 4 th lack of pseudo-ginseng powder, the 5 th lack of sodium humate and the 6 th lack of taurine are all replaced by corn starch. The 6 single component missing additive formulations are shown in table 3.
The culture test was carried out on the fish raft in the sea area of the Changhui island, xi nan province, xia county, nippon city, ningde, fujian province from 5/month 2/2018 to 27/month 27/2018. Selecting and transferring apparent healthy large yellow croaker 67200 tails with uniform specifications into 8 test net cages (8 m x 4m x 6m, water depth: 5.6 m), randomly dividing each net cage 3200 tail into 7 groups, and feeding the feeds of the embodiment 1 and the deletion examples 1-6 (the feed prepared from 1 part of premix and 99 parts of the feed of the comparative example 1) at 3 times of each group, wherein the feeding is performed at a regular time of 5 parts per day and 17 parts per day, and the feeding lasts at least half an hour, so that the fish is apparently satiated. And (5) recording the daily food intake, the number of dead fishes (the number of fish died due to white spot disease is distinguished) and the weight of the dead fishes per net cage. The test period is 56 days, and the fish in each net cage is counted and weighed when the test is finished, and whether the fish suffers from white spot disease is observed and recorded. The test results are shown in Table 4.
Table 3:
| example 1 | Deficiency example 1 | Deletion example 2 | Deletion example 3 | Deletion example 4 | Deletion example 5 | Deletion example 6 | |
| Eucalyptus oil | 10 | 0 | 10 | 10 | 10 | 10 | 10 |
| Hesperidin | 10 | 10 | 0 | 10 | 10 | 10 | 10 |
| Pine pollen | 30 | 30 | 30 | 0 | 30 | 30 | 30 |
| Notoginseng powder | 15 | 15 | 15 | 15 | 0 | 15 | 15 |
| Humic acid sodium salt | 15 | 15 | 15 | 15 | 15 | 0 | 15 |
| Taurine | 20 | 20 | 20 | 20 | 20 | 20 | 0 |
| Corn starch | 0 | 10 | 10 | 30 | 15 | 15 | 30 |
| Total up to | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Table 4:
| example 1 | Deficiency example 1 | Deficiency example 2 | Deletion example 3 | Deletion example 4 | Deficiency example 5 | Deletion example 6 | |
| Initial body weight (g) | 125.6±0.4 | 124.9±0.3 | 125.3±0.5 | 124.7±0.4 | 126.2±0.3 | 125.3±0.3 | 125.8±0.5 |
| Terminal body weight (g) | 220.9±2.1a | 208.4±2.0c | 212.3±1.9b | 208.5±2.5c | 215.7±1.6ab | 213.8±2.2bc | 212.6±1.4bc |
| Percentage of increase (%) | 75.9±0.9a | 65.3±0.8c | 69.4±0.7b | 67.2±0.9bc | 70.9±0.6b | 70.6±0.9b | 69.0±0.6b |
| Feeding amount (g) | 135.9±1.4 | 136.2±1.5 | 135.6±1.8 | 132.3±2.4 | 134.8±1.3 | 136.2±1.0 | 136.0±0.8 |
| Coefficient of feed | 1.43±0.02a | 1.63±0.02b | 1.56±0.01b | 1.58±0.02b | 1.51±0.03b | 1.54±0.02b | 1.57±0.01b |
| Total mortality (%) | 2.4±0.3a | 12.8±1.1b | 10.1±1.5b | 9.8±0.9b | 12.9±2.2b | 11.6±1.6b | 9.5±1.3b |
| Incidence of white Point disease (%) | 3.2±0.6a | 32.4±6.1b | 26.8±5.6b | 25.5±4.8b | 33.5±6.3b | 30.7±5.3b | 24.6±4.2b |
| White Point disease mortality (%) | 1.0±0.2a | 10.3±2.0b | 9.2±1.8b | 8.8±1.6b | 11.2±2.1b | 10.3±2.3b | 8.5±1.7b |
As can be seen from the test data in Table 4, the terminal weight and the weight gain rate of the composition in example 1 are significantly higher than those of the compositions in the deficiency examples 1-6, and the feed coefficient, the total mortality, the morbidity of ichthyophthiriasis and the mortality of ichthyophthiriasis are significantly lower than those of the compositions in the deficiency examples 1-6. The comparison results show that the components in the additive have synergistic effect.
In addition, the inventor further researches the effect of the weight ratio of the components in the premix of the invention by similar experiments, and proves that the feed coefficient, the mortality, the morbidity of ichthyophthiriasis, the mortality of ichthyophthiriasis and the like are better by adopting the weight ratio, and the premix effect is remarkably improved by the weight ratio.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.
Claims (9)
1. The premix for preventing and treating the marine fish ichthyophthiriasis is characterized by comprising the following components in parts by weight: 5-10 parts of eucalyptus oil, 10-15 parts of hesperidin, 25-35 parts of pine pollen, 15-25 parts of pseudo-ginseng powder, 10-20 parts of sodium humate and 10-20 parts of taurine.
2. The premix for preventing and treating the white spot disease of the marine fish according to claim 1, which is characterized by comprising the following components in parts by weight: 10 parts of eucalyptus oil, 10 parts of hesperidin, 30 parts of pine pollen, 15 parts of pseudo-ginseng powder, 15 parts of sodium humate and 20 parts of taurine.
3. The premix for preventing and treating the white spot disease of the marine fish as claimed in claim 1, which is characterized by comprising the following components in parts by weight: 5 parts of eucalyptus oil, 15 parts of hesperidin, 25 parts of pine pollen, 20 parts of pseudo-ginseng powder, 20 parts of sodium humate and 15 parts of taurine.
4. The premix for preventing and treating the white spot disease of the marine fish according to claim 1, which is characterized by comprising the following components in parts by weight: 8 parts of eucalyptus oil, 12 parts of hesperidin, 35 parts of pine pollen, 25 parts of pseudo-ginseng powder, 10 parts of sodium humate and 10 parts of taurine.
5. A method for preparing the premix for the marine fish for preventing and treating ichthyophthiriasis of any one of claims 1 to 4, which comprises the following steps:
(1) Mixing hesperidin, pollen pini, pseudo-ginseng powder, sodium humate and taurine uniformly to obtain a mixture;
(2) Micronizing the mixture;
(3) Adding eucalyptus oil, and mixing to obtain premix.
6. The method of claim 5, comprising the steps of:
(1) Evenly mixing hesperidin, pollen pini, pseudo-ginseng powder, sodium humate and taurine to obtain a mixture;
(2) Micronizing the mixture, and sieving with 100 mesh sieve;
(3) Adding eucalyptus oil, and mixing to obtain premix.
7. The premix for marine fish prepared according to the preparation method of claim 5 or 6.
8. The application of the premix for preventing and treating the white spot disease of the marine fish in the preparation of the compound feed for the marine fish is characterized in that the premix is the premix according to any one of claims 1-4 and 7, and the addition amount of the premix is 0.5-2% of the weight percentage of the compound feed for the marine fish.
9. Use according to claim 8, wherein the amount added is 1% by weight of the feed composition for marine fish.
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| CN112690375B true CN112690375B (en) | 2022-10-11 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09301807A (en) * | 1996-05-09 | 1997-11-25 | Morinaga Milk Ind Co Ltd | Agent for preventing and treating parasitic disease of aquatic animal |
| JP2011153103A (en) * | 2010-01-28 | 2011-08-11 | Univ Of Tokyo | Agent for preventing and treating ichthyophthiriasis of marine fish |
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| CN104412925A (en) * | 2013-09-06 | 2015-03-18 | 贾友宏 | Sea fish ichthyophthiriasis prevention and treatment method |
| CN104621023B (en) * | 2015-03-13 | 2017-01-11 | 福建省农业科学院中心实验室 | Method for preventing and treating ichthyophthirius multifiliis of maccullochella peelii |
| CN105341534A (en) * | 2015-11-30 | 2016-02-24 | 青岛海之源智能技术有限公司 | Feed additive for preventing white-spot disease of parrot fishes and preparation method thereof |
| CN107821772A (en) * | 2017-10-31 | 2018-03-23 | 全椒县鮰鱼养殖专业合作社 | A kind of special feed of Ti Gao Channel-catfish fish survival rates |
| CN110438015B (en) * | 2019-09-04 | 2022-09-16 | 桂林理工大学 | Immature bitter orange endophytic fungus for producing hesperidinase and method for producing hesperidinase by fermenting immature bitter orange endophytic fungus |
| CN111418735A (en) * | 2020-04-22 | 2020-07-17 | 广东汇海农牧科技集团有限公司 | Feed for preventing trachinotus ovatus body surface parasites and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09301807A (en) * | 1996-05-09 | 1997-11-25 | Morinaga Milk Ind Co Ltd | Agent for preventing and treating parasitic disease of aquatic animal |
| JP2011153103A (en) * | 2010-01-28 | 2011-08-11 | Univ Of Tokyo | Agent for preventing and treating ichthyophthiriasis of marine fish |
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