CN112690281B

CN112690281B - Nematicidal pesticide composition and preparation and application thereof

Info

Publication number: CN112690281B
Application number: CN202011558524.4A
Authority: CN
Inventors: 唐兴敏; 王澍春; 陈海涛; 柴方堃; 贾成国
Original assignee: Hebei Veyong Bio Chemical Co ltd
Current assignee: Hebei Veyong Bio Chemical Co ltd
Priority date: 2020-12-25
Filing date: 2020-12-25
Publication date: 2021-10-01
Anticipated expiration: 2040-12-25
Also published as: CN112690281A

Abstract

The invention relates to the technical field of pesticide preparation, and particularly discloses a nematicidal pesticide composition and a preparation and application thereof. According to the nematicidal pesticide composition provided by the invention, the fluopyram and the menthyl acetate are compounded as main components, so that the control effect of plant insect pests caused by root-knot nematodes can be remarkably enhanced, the action mechanisms of the two components are different from each other, the single selection pressure on the nematodes is reduced, the generation of nematode resistance is favorably overcome, the generation speed of the nematode drug resistance is delayed, meanwhile, the menthyl acetate has soft mint and rose fragrance, the feeding behavior of the nematodes can be induced, and the insecticidal effect is further improved. The insecticidal composition provided by the invention has the advantages that the prevention and control effect on the pests is obviously improved by selecting raw materials with different action mechanisms for compounding, the duration is long, the pests are not easy to generate drug resistance after long-term use, the dosage can be reduced, the cost is saved, the pollution to the environment is reduced, and the popularization value is great.

Description

Nematicidal pesticide composition and preparation and application thereof

Technical Field

The invention relates to the technical field of pesticide preparation, and particularly relates to a nematicidal pesticide composition and a preparation and application thereof.

Background

Fluopyram, chemical name: n- {2- [ 3-chloro-5- (trifluoromethyl) -2-pyridyl ] ethyl } -alpha, alpha-trifluoro-O-toluamide is a pesticide with a novel action mechanism, and belongs to succinate dehydrogenase inhibitors as bactericidal and nematocidal agents. The bactericide composition is mainly used for preventing and controlling various plant diseases caused by fungal pathogens, such as alternaria leaf spot, gray mold, powdery mildew, sclerotinia rot and early blight peaks of vegetables such as grapes, pear trees, bananas, apples, cucumbers and tomatoes and field crops, and can be registered on various crops for preventing and controlling various nematodes. However, the single fluopyram medicament has an unsatisfactory effect of killing nematodes and a short duration. Therefore, in order to effectively control diseases and protect the ecological environment, reduce the dosage of pesticides and reduce pesticide residues while effectively controlling the harm of nematodes, the research and the development of a pollution-free biological pesticide with good nematode killing activity and long lasting effect is of great significance.

Disclosure of Invention

Aiming at the problems of unsatisfactory effect and poor persistence of fluopyram on nematode control in the prior art, the invention provides a nematode-killing pesticide composition, and a preparation and application thereof.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

a nematicidal pesticide composition comprises fluopyram as an effective component and menthyl acetate as a synergist.

Compared with the prior art, the nematicidal pesticide composition provided by the invention has the advantages that the fluopyram and the menthyl acetate are compounded as main components, the fluopyram and the menthyl acetate can generate a synergistic effect, the prevention and treatment effect of plant insect pests caused by root-knot nematodes can be obviously enhanced, the action mechanisms of the two components are different from each other, the single selection pressure on the nematodes is reduced, the generation of nematode resistance is favorably overcome, the generation speed of the drug resistance of the nematodes is delayed, meanwhile, the menthyl acetate has soft mint and rose fragrance, the feeding behavior of the nematodes can be induced, and the insecticidal effect is further improved. The nematicidal composition provided by the invention has the advantages that by selecting raw materials with different action mechanisms for compounding, the prevention and treatment effect on nematodes is remarkably improved, the lasting period of pesticides is prolonged, the dosage and the application frequency of the pesticides are reduced, the pollution to the environment is reduced, and the nematicidal composition is particularly suitable for preventing and treating nematodes of vegetable crops, grain crops, fruit trees, flowers and the like and has great popularization value.

Fluopyram is a novel benzamide medicament, is mainly used for preventing and treating various plant diseases caused by fungal pathogens, such as gray mold, powdery mildew, late blight, downy mildew, rice blast, sclerotinia sclerotiorum and the like, and can also be used for preventing and treating plant nematodes, and the insecticidal mechanism is that succinate dehydrogenase or succinate coenzyme Q reductase acts on a mitochondrial respiration electron transfer chain, and the mitochondrial respiration is inhibited by blocking electron transfer in the respiratory chain of the succinate dehydrogenase. However, the single fluopyram has undesirable control effect, short duration and is easy to generate drug resistance.

Menthyl acetate has soft mint and rose fragrance, is mainly used for preparing spices, essence and the like at present, is widely applied to the fields of various foods, beverages and cosmetics, but has not been reported to be applied to the field of pesticides.

Preferably, the mass ratio of fluopyram to menthyl acetate is 20:1-1: 20.

More preferably, the mass ratio of fluopyram to menthyl acetate is 5:1-1: 5.

Under the condition of the optimal mass ratio, the fluopyram and the menthyl acetate have very obvious synergistic effect, have excellent control effect on plant nematodes and can obviously prolong the lasting period.

The invention also provides a nematicide comprising the nematicide pesticide composition.

Preferably, the nematicide also comprises an auxiliary agent, and the content of the total mass of the fluopyram and the menthyl acetate in the nematicide is 30-50 wt%.

The preferable content is favorable for obtaining higher control effect on the premise of less pesticide dosage.

Preferably, the auxiliary agent comprises at least one of a cosolvent, a dispersing agent, a wetting agent, a defoaming agent, a disintegrating agent, a thickening agent or a filler.

In order to prepare the insecticidal composition into various formulations, a plurality of auxiliary agents are required to be added, and the cosolvent, the dispersant, the wetting agent, the defoamer, the disintegrant, the thickener or the filler are all commonly used in the field of pesticides, and different auxiliary agents can be selected according to different formulations, and specific components and dosage can be determined through routine experiments according to formulation requirements.

Optionally, the nematicide is in the form of a suspending agent, an aqueous emulsion, a microemulsion, an emulsifiable concentrate, a wettable powder, a water dispersible granule or a granule.

The preparation can be prepared by selecting conventional additives in the field of pesticides according to a conventional preparation process. The pesticide composition is prepared into various dosage forms, is convenient to use, is suitable for various application modes, meets different control and use requirements, and has a wide application range.

The invention also provides application of the nematicidal pesticide composition in prevention and treatment of plant nematodes.

The invention also provides application of the nematicide of any one of the above items in controlling plant nematodes.

The nematicidal composition or nematicide is generally applied by diluting and then irrigating roots, and the application amount and dilution factor of the nematicide are changed along with the change of biological characteristics or environmental conditions of a control object.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In order to better illustrate the invention, the following examples are given by way of further illustration.

Example 1

This example provides a nematicidal suspension:

the composition is prepared according to the mass percentage of 5:1 of fluopyram and menthyl acetate. 40.0 percent of fluopyram, 8.0 percent of menthyl acetate, 3.0 percent of sodium polycarboxylate, 2.0 percent of EO-PO block polymer, 2.0 percent of sodium lignosulfonate, 0.2 percent of sodium benzoate, 0.3 percent of organic silicon defoamer, 1.0 percent of magnesium aluminum silicate, 0.3 percent of xanthan gum, 5.0 percent of ethylene glycol and deionized water are added to make up to 100 percent. The above components are mixed evenly, and the nematicidal suspending agent is obtained after grinding and high-speed shearing.

Example 2

This example provides a nematicidal microemulsion:

the composition is prepared according to the mass percentage of 1:1 of fluopyram and menthyl acetate. 20.0 percent of fluopyram, 20.0 percent of menthyl acetate, 10.0 percent of S-150 solvent oil, 15.0 percent of fatty alcohol-polyoxyethylene ether, 8.0 percent of alkylphenol polyoxyethylene phosphate, 5.0 percent of glycol and deionized water are taken to be supplemented to 100 percent.

And (3) uniformly mixing the components except the deionized water, and adding the deionized water under the stirring condition to obtain the nematode-killing microemulsion.

Example 3

The embodiment provides a nematicidal water dispersible granule:

the composition is prepared according to the mass percentage of fluopyram to menthyl acetate of 1: 5. 8.0 percent of fluopyram, 40.0 percent of menthyl acetate, 4.0 percent of sodium polycarboxylate, 6.0 percent of sodium dodecyl sulfate, 3.0 percent of sodium lignosulfonate, 18 percent of corn starch and diatomite are taken to complement to 100 percent.

The components are uniformly mixed, are crushed into wettable powder through airflow, are added with water to be mixed and extruded for material making, and are dried and screened to obtain the nematicidal water dispersible granule.

Example 4

The embodiment provides a nematicidal wettable powder:

the composition is prepared according to the mass percentage of 20:1 of fluopyram and menthyl acetate. Taking fluopyram 40.0%, menthyl acetate 2.0%, sodium high molecular lignin sulfonate 5.0%, sodium dodecyl sulfate 2.0%, NNO dispersant 5.0%, and kaolin to complement to 100%.

The components are uniformly mixed, and are crushed by air flow until the fineness is qualified, so that the nematicidal wettable powder is obtained.

Example 5

The embodiment provides a nematicidal emulsifiable concentrate:

the composition is prepared according to the mass percentage of fluopyram to menthyl acetate of 1: 19.6. Taking fluopyram 1.7%, menthyl acetate 33.3%, tetrahydrofuran 8.0%, nonylphenol polyoxyethylene ether 15.0%, phenethyl phenol polyoxyethylene ether phosphate 20.0%, and xylene to complement to 100%. And (3) uniformly mixing the components to obtain the nematicidal emulsifiable solution.

The insecticidal composition provided by the invention can be prepared into other dosage forms such as aqueous emulsion or granules by selecting conventional auxiliary materials in the field of pesticides according to a conventional process, and the other dosage forms with the same effective component content can achieve the effect of killing nematodes basically the same as the dosage forms in examples 1-5.

Comparative example 1

The comparative example uses the same raw materials and preparation method as those in example 2, except that the menthyl acetate in example 2 is replaced by the same amount of fluopyram to prepare the corresponding nematicidal microemulsion.

Comparative example 2

The comparative example uses the same raw materials and preparation method as those in example 2, except that the flupyrad in example 2 is replaced by the same amount of menthyl acetate to prepare the corresponding nematicidal microemulsion.

Comparative example 3

This comparative example used the same raw materials and preparation methods as in example 2, except that the menthyl acetate in example 2 was replaced with an equal amount of ethyl methanesulfonate to prepare the corresponding nematicidal microemulsion.

Comparative example 4

The comparative example adopts the same raw materials and preparation method as those in example 2, except that the flupyrad in example 2 is replaced by the same amount of deionized water to prepare the corresponding nematicidal microemulsion.

Comparative example 5

This comparative example used the same raw materials and preparation methods as in example 2, except that the menthyl acetate in example 2 was replaced with an equivalent amount of deionized water to prepare the corresponding nematicidal microemulsion.

In order to determine the nematicidal effect of the product, indoor biological activity determination and field efficacy test are carried out.

Indoor biological activity assay

Test subjects: root knot nematode of tomato

The test method comprises the following steps: 6mL of 240-plus 260-head/mL nematode second-instar larva suspension is put into a culture dish (the diameter is 6cm), the test agents with the same volume series concentration are added, the final concentration of the test agents is set as the experimental concentration, each treatment is repeated for 4 times, and clear water is used as a blank control. Culturing at 25 deg.C for 36h, examining activity of nematode, and calculating relative lethality of each agent treatment to second-instar larva of nematode.

Dead nematodes are stiff and bent into bow or spiral, and live nematodes are mostly in bending, moving and twisting states. If the dead and alive state of the stiff nematodes is difficult to judge, the nematodes to be identified can be placed in a 2% sodium chloride solution, and after 2min, the state of the nematodes is observed, the stiff nematodes are dead, and the activity of the nematodes curls or twists to be alive.

The evaluation of the effect of the combination of the mixtures was determined according to the Gowing method, which is formulated as:

Eo＝X+Y-XY/100

evaluation criteria: when E-Eo > 10%, indicating a synergistic effect; when the E-Eo is more than or equal to 10 percent and less than or equal to 10 percent, a synergistic and additive effect is shown; when E-Eo < -10%, it is expressed as a synergistic antagonism.

In the formula, Eo is a theoretical value or an expected value of the control effect on the target pests after the components A and B are mixed according to a certain proportion, X is the percentage control effect when the component A is used alone, Y is the percentage control effect when the component B is used alone, and E is an actual value or an observed value of the control effect on the target pests after the components A and B are mixed according to a certain proportion.

TABLE 1 synergistic assay results

Note: in the above table, A represents fluopyram and B represents menthyl acetate.

As can be seen from the comparison of the data in Table 1 between examples 1-5 and comparative examples 4-5, the combination of fluopyram and menthyl acetate has a synergistic effect on the root-knot nematodes of tomato.

Test of field drug effect

The experiment is carried out in a longevity vegetable greenhouse in the garden of Shandong province and the Weifang city, the root irrigation method is adopted, the selected cucumbers grow basically in the same way and are in the vegetative growth period, and the nematodes are more serious in the past year. The experiment is provided with 11 treatments, each treatment is repeated for 3 times, and 33 treatment cells are provided (the nematode damage in the treatment zones is basically uniform, and the ecological environment is the same). After the treatment of the medicament, a random sampling method is adopted, sampling is carried out at 5 points in each cell, 4 plants are randomly investigated at each point, 20 plants are counted, roots are dug out, and the harm level of the root-knot nematodes is investigated and recorded.

The investigation method is carried out according to the pesticide field efficacy test criteria.

Level 0: no visible nodules or egg masses;

level 1: the total length of the root nodules accounts for 1% -24% of the total root length;

and 2, stage: the total length of the root nodules accounts for 25% -49% of the total root length;

and 3, level: the total length of the root nodules accounts for 50% -74% of the total root length;

4, level: the total length of the root nodules accounts for 75-100% of the total root length;

disease index ═ Σ (number of disease roots at each stage × number of relative stage)/(total number of investigated plants × 4) × 100;

the preventing and treating effect (%) is (1-treatment disease index/control disease index) × 100.

The damage condition of cucumber roots is checked at 30d and 60d after the application of the medicine respectively. The test results are shown in table 2.

TABLE 2 field control of cucumber root-knot nematodes

Note: the effective component content refers to the total amount of fluopyram and menthyl acetate.

Test results show that the fluopyram and menthyl acetate composition has a good effect of preventing and treating cucumber root-knot nematodes, has an obvious synergistic effect, prolongs the lasting period, improves the utilization rate of effective components, can reduce the dosage, saves the cost and reduces the pollution to the environment.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. A nematicidal pesticide composition is characterized in that the effective components comprise fluopyram, and the synergist comprises menthyl acetate; the mass ratio of fluopyram to menthyl acetate is 20:1-1: 20.

2. The nematicidal pesticide composition according to claim 1, wherein the mass ratio of fluopyram to menthyl acetate is 5:1 to 1: 5.

3. A nematicide comprising the nematicidal pesticide composition of any one of claims 1 to 2.

4. The nematicide of claim 3, further comprising an auxiliary agent, wherein the total mass of fluopyram and menthyl acetate is 30 to 50 wt% in the nematicide.

5. The nematicide of claim 4, wherein the adjuvant comprises at least one of a cosolvent, a dispersant, a wetting agent, a defoamer, a disintegrant, a thickener or a filler.

6. The nematicide of claim 3, wherein the nematicide is in the form of a suspension, an aqueous emulsion, a microemulsion, an emulsifiable concentrate, a wettable powder, a water dispersible granule or a granule.

7. Use of the nematicidal pesticide composition of any one of claims 1 to 2 to control plant nematodes.

8. Use of the nematicide of any one of claims 3 to 6 for controlling plant nematodes.