CN112690170A - Culture medium for cultivating black fungus and preparation method thereof - Google Patents
Culture medium for cultivating black fungus and preparation method thereof Download PDFInfo
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- CN112690170A CN112690170A CN202011545343.8A CN202011545343A CN112690170A CN 112690170 A CN112690170 A CN 112690170A CN 202011545343 A CN202011545343 A CN 202011545343A CN 112690170 A CN112690170 A CN 112690170A
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- 241000233866 Fungi Species 0.000 title claims abstract description 70
- 239000001963 growth medium Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 54
- 239000000843 powder Substances 0.000 claims abstract description 37
- 239000007787 solid Substances 0.000 claims abstract description 34
- 239000010902 straw Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 16
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 16
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 16
- 239000010440 gypsum Substances 0.000 claims abstract description 16
- 239000004571 lime Substances 0.000 claims abstract description 16
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 15
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 11
- 235000013312 flour Nutrition 0.000 claims abstract description 10
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims description 48
- 238000004659 sterilization and disinfection Methods 0.000 claims description 45
- 239000000463 material Substances 0.000 claims description 39
- 238000005303 weighing Methods 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 244000020551 Helianthus annuus Species 0.000 claims description 2
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 239000002023 wood Substances 0.000 abstract description 11
- 239000002154 agricultural waste Substances 0.000 abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 3
- 230000002378 acidificating effect Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000011575 calcium Substances 0.000 abstract description 3
- 229910052791 calcium Inorganic materials 0.000 abstract description 3
- 239000011593 sulfur Substances 0.000 abstract description 3
- 229910052717 sulfur Inorganic materials 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- 208000012868 Overgrowth Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for cultivating black fungus and a preparation method thereof, wherein the culture medium is prepared from solid raw materials and water according to the weight ratio of 1:1.1-1.4, the pH value of the culture medium is 7-7.5, and the solid raw materials comprise the following raw materials in parts by weight: 37-49 parts of broad-leaved tree sawdust, 28-35 parts of corncobs, 10-15 parts of crop straws, 9-10 parts of wheat bran, 1.7-2.3 parts of bean flour, 0.5-1 part of gypsum powder and 0.5-1 part of lime powder. The gypsum powder can provide calcium and sulfur elements and also has the function of adjusting the pH value, and the lime powder can prevent the pH value of the culture medium from being reduced after the culture medium becomes acidic. The invention utilizes agricultural wastes such as corncobs, crop straws and the like to replace wood chips, solves the problem of shortage of the wood chip cultivation raw materials of the black fungus, and is beneficial to promoting the sustainable and stable development of the black fungus industry.
Description
Technical Field
The invention relates to the technical field of fungus cultivation, in particular to a culture medium for cultivating black fungus and a preparation method thereof.
Background
At present, the black fungus production in China mainly adopts a plastic bag substitute cultivation method, the existing black fungus cultivation technology adopts broad-leaved tree sawdust, wheat bran, bean cake powder and other agricultural and forestry byproducts as main raw materials, wherein the broad-leaved tree sawdust accounts for 90 parts of the weight of the raw materials, and the formula comprises the following main components in percentage by weight: 90 parts of broad-leaved tree sawdust, 8 parts of wheat bran and 2 parts of bean powder. In recent years, with the implementation of ecological environment protection and the policy of comprehensively forbidding forestry commercial felling in China, the wood chip raw material for cultivating and producing black fungus is greatly reduced, the contradiction between supply and demand of raw materials is increasingly prominent, and the sustainable development of the black fungus industry is not facilitated.
Disclosure of Invention
In view of the above, the invention aims to provide a culture medium for cultivating black fungus and a preparation method thereof, agricultural wastes such as corncobs and corn straws are used for replacing wood chips, so that the problem of shortage of wood chip cultivation raw materials of black fungus is solved, and the sustainable and stable development of the black fungus industry is promoted.
In order to achieve the purpose of the invention, the technical scheme is as follows:
the culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.1-1.4, and the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 37-49 parts of broad-leaved tree sawdust, 28-35 parts of corncobs, 10-15 parts of crop straws, 9-10 parts of wheat bran, 1.7-2.3 parts of bean flour, 0.5-1 part of gypsum powder and 0.5-1 part of lime powder.
In the culture medium for culturing the black fungus, the gypsum powder is added to provide calcium and sulfur elements and also play a role in regulating the pH value, and the lime powder is added to prevent the pH value of the culture medium from being reduced after the culture medium becomes acidic.
As a further improvement of the present invention, in the above technical solution, the weight ratio of the solid raw material to water is preferably 1:1.3, wherein the solid raw material comprises the following raw materials in parts by weight: 45 parts of broad-leaved tree sawdust, 30 parts of corncobs, 12 parts of crop straws, 9 parts of wheat bran, 2 parts of soybean powder, 1 part of gypsum powder and 1 part of lime powder.
As a further improvement of the present invention, in the above technical scheme, the crop straw is one or a combination of corn straw, beanstalk, sunflower stalk, wheat straw and rice straw.
A preparation method of a culture medium for cultivating black fungus comprises the following steps:
(1) crushing: respectively crushing broad-leaved tree sawdust, corncobs and crop straws into particles with the diameter less than 0.3 cm;
(2) weighing: weighing 40-50 parts of broad-leaved tree sawdust, 25-35 parts of corncobs, 10-14 parts of crop straws, 8-10 parts of wheat bran, 1.5-2.5 parts of bean flour, 0.8-1.2 parts of gypsum powder and 0.8-1.2 parts of lime powder according to the weight ratio, uniformly mixing to obtain a solid raw material, putting the solid raw material into a stirrer, adding water into the stirrer according to the weight ratio of the solid raw material to the water of 1:1.1-1.4, controlling the pH value of a mixture of the solid raw material and the water to be 7-7.5, and uniformly stirring to obtain a mixed material;
(3) bagging: bagging the mixed material in the step (2), and using a high-temperature-resistant and high-pressure-resistant polypropylene corner folding plastic bag with the specification of 17cm multiplied by 36cm multiplied by 0.04cm or 17cm multiplied by 38cm multiplied by 0.04 cm; when the fungus bags are bagged, the tightness is required to be proper, the surface of the fungus bags is generally hard and elastic, and the fungus bags can be bounced when the thumb lightly presses the surface of the fungus bags; or the tightness of the fungus bag is measured by weighing the weight of the fungus bag by a scale, and the weight of the fungus bag is about 1.2 kg. According to the requirement of the charging quality, the damage, the crack and the bulge of the material bag can not occur after the material bag is filled, the material bag is not overhigh, the bottom of the bag is flat, the outer wall of the bag is smooth, the height of the bag is uniform, and the weight error is controlled within +/-0.1 kilogram.
(4) Screwing the opening: preparing a plastic core rod in advance by adopting a professional socket machine, placing the material bag loaded in the step (3) on a rotary table, putting a central drill in the center of the material bag by a right hand, binding the material ports together by the left hand by taking the central drill as a rotary shaft, treading a rotator by the right hand to rotate the rotary table, simultaneously, forcibly plugging the material ports into the center of the material bag by the right hand downwards, treading down the left foot, lifting the right foot, pulling out the central drill while reversing the rotary table, inserting a bacterium stick, and plugging a sponge cover body at a socket;
(5) and (3) sterilization: sterilizing the material bag screwed in the step (4), wherein the sterilization is mainly used for killing harmful bacteria and other microorganisms including propagules and spores in the culture material and promoting partial decomposition of organic substances of the culture material;
(6) and (3) cooling: after sterilization, the fungus bags are taken out from the sterilization pot, cooled to below 30 ℃, and then transferred into an inoculation room for inoculation and culture under aseptic conditions.
As a further improvement of the present invention, when sterilization is performed in step (5), either of two methods, atmospheric steam sterilization and high-pressure steam sterilization, may be used;
wherein, the normal pressure steam sterilization adopts a normal pressure sterilization pot, the material bag is put into the sterilization pot and then is fiercely burnt by strong fire, the pot is required to be boiled within 3 to 4 hours, and the sterilization is thoroughly kept for 10 to 12 hours;
the high-pressure steam sterilization adopts a high-pressure steam sterilization pot, cold air is required to be discharged firstly during high-pressure sterilization, and when the pressure reaches 0.153MPa and the steam temperature is 128.1 ℃, the steam is kept for 2.5 to 3 hours, so that the aim of sterilization can be fulfilled.
The invention has the beneficial effects that:
(1) the fungus bag fungus-growing period is shortened: the culture period from inoculation culture to hypha overgrowth of the fungus bags is only about 25 days, and is reduced by 3-5 days compared with the production period of a pure wood chip culture medium formula;
(2) the yield is stable: the hypha growth of the fungus bags is uniform, and the difference of the fungus yield and the fungus bags of the pure wood chip culture medium formula is not large;
(3) the raw material cost is reduced, and the production cost is reduced by 30 parts compared with the fungus bag production cost of a pure wood chip culture medium formula.
Detailed Description
The following examples are intended to further illustrate the present invention and should not be construed as limiting the scope of the invention, and other insubstantial modifications and adaptations of the invention by those skilled in the art based on the teachings herein are intended to be covered thereby.
The invention utilizes agricultural wastes such as corncobs, crop straws and the like to replace wood chips, solves the problem of shortage of the wood chip cultivation raw materials of the black fungus, and is beneficial to promoting the sustainable and stable development of the black fungus industry. In the culture medium for cultivating the black fungus, the gypsum powder is added to provide calcium and sulfur elements and also play a role in adjusting the pH value, and the lime powder is added to prevent the pH value of the culture medium from being reduced after the culture medium becomes acidic.
Example 1
The culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.3, the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 45 parts of broad-leaved tree sawdust, 30 parts of corncobs, 12 parts of corn straws, 9 parts of wheat bran, 2 parts of bean flour, 1 part of gypsum powder and 1 part of lime powder.
A preparation method of a culture medium for cultivating black fungus comprises the following steps:
(1) crushing: respectively crushing broad-leaved tree sawdust, corncobs and corn straws into particles with the diameter less than 0.3 cm;
(2) weighing: weighing 45 parts of broad-leaved tree sawdust, 30 parts of corncobs, 12 parts of corn straws, 9 parts of wheat bran, 2 parts of bean flour, 1 part of gypsum powder and 1 part of lime powder according to the weight ratio, uniformly mixing to obtain a solid raw material, putting the solid raw material into a stirrer, adding water into the stirrer according to the weight ratio of the solid raw material to the water of 1:1.3, controlling the pH value of a mixture of the solid raw material and the water to be 7-7.5, and uniformly stirring to obtain a mixed material;
(3) bagging: bagging the mixed material in the step (2), and using a high-temperature-resistant and high-pressure-resistant polypropylene corner folding plastic bag with the specification of 17cm multiplied by 36cm multiplied by 0.04cm or 17cm multiplied by 38cm multiplied by 0.04 cm; when the fungus bags are bagged, the tightness is required to be proper, the surface of the fungus bags is generally hard and elastic, and the fungus bags can be bounced when the thumb lightly presses the surface of the fungus bags; or the tightness of the fungus bag is measured by weighing the weight of the fungus bag by a scale, and the weight of the fungus bag is about 1.2 kg. According to the requirement of the charging quality, the damage, the crack and the bulge of the material bag can not occur after the material bag is filled, the material bag is not overhigh, the bottom of the bag is flat, the outer wall of the bag is smooth, the height of the bag is uniform, and the weight error is controlled within +/-0.1 kilogram.
(4) Screwing the opening: preparing a plastic core rod in advance by adopting a professional socket machine, placing the material bag loaded in the step (3) on a rotary table, putting a central drill in the center of the material bag by a right hand, binding the material ports together by the left hand by taking the central drill as a rotary shaft, treading a rotator by the right hand to rotate the rotary table, simultaneously, forcibly plugging the material ports into the center of the material bag by the right hand downwards, treading down the left foot, lifting the right foot, pulling out the central drill while reversing the rotary table, inserting a bacterium stick, and plugging a sponge cover body at a socket;
(5) and (3) sterilization: sterilizing the material bag screwed in the step (4), wherein the sterilization is mainly used for killing harmful bacteria and other microorganisms including propagules and spores in the culture material and promoting partial decomposition of organic substances of the culture material;
(6) and (3) cooling: after sterilization, the fungus bags are taken out from the sterilization pot, cooled to below 30 ℃, and then transferred into an inoculation room for inoculation and culture under aseptic conditions.
During sterilization in the step (5), either one of normal pressure steam sterilization and high pressure steam sterilization can be adopted;
wherein, the normal pressure steam sterilization adopts a normal pressure sterilization pot, the material bag is put into the sterilization pot and then is fiercely burnt by strong fire, the pot is required to be boiled within 3 to 4 hours, and the sterilization is thoroughly kept for 10 to 12 hours;
the high-pressure steam sterilization adopts a high-pressure steam sterilization pot, cold air is required to be discharged firstly during high-pressure sterilization, and when the pressure reaches 0.153MPa and the steam temperature is 128.1 ℃, the steam is kept for 2.5 to 3 hours, so that the aim of sterilization can be fulfilled.
Example 2
The culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.3, the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 45 parts of broad-leaved tree wood chips, 30 parts of corncobs, 12 parts of beanstalks, 9 parts of wheat bran, 2 parts of soybean flour, 1 part of gypsum powder and 1 part of lime powder.
The preparation method of the culture medium for cultivating black fungus is the same as that of the example 1.
Example 3
The culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.1, the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: broad-leaved tree sawdust 37 parts, corncob 28 parts, beanstalk 10 parts, wheat bran 9 parts, soybean flour 1.7 parts, gypsum powder 0.5 part, and lime powder 0.5 part.
The preparation method of the culture medium for cultivating black fungus is basically the same as that of the example 1, and the difference is that: adding water into the stirrer according to the weight ratio of the solid raw material to the water of 1: 1.1.
Example 4
The culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.4, the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 49 parts of broad-leaved tree sawdust, 35 parts of corncobs, 15 parts of wheat straws, 10 parts of wheat bran, 2.3 parts of bean flour, 0.9 part of gypsum powder and 0.8 part of lime powder.
The preparation method of the culture medium for cultivating black fungus is the same as that of the example 1, and the difference is that: adding water into the stirrer according to the weight ratio of the solid raw material to the water of 1: 1.4.
Comparative example 1
The culture medium for cultivating the black fungus is prepared from solid raw materials and water according to the weight ratio of 1:1.3, the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 87 parts of broad-leaved tree sawdust, 9 parts of wheat bran, 2 parts of bean powder, 1 part of gypsum powder and 1 part of lime powder.
The preparation method of the culture medium for cultivating black fungus is the same as that of the example 1.
The black fungus cultivation test is carried out by taking the comparative example 1 as a control group and the examples 1-2 as experimental groups, and the comparison test is carried out by taking the spawn running time, the fungus sack pollution rate, the yield, the fungus emergence time and the ear morphology as indexes, and the test results are shown in table 1:
TABLE 1 test results
Compared with the comparative example 1, the fungus growing time and the fungus growing time are both reduced, the cultivation period is shortened by 7 days, the fungus sack pollution rate is reduced by 0.2%, the average single-sack yield is not high in contrast but the difference is not obvious, the ear sizes are uniform, and the mouth feel is better; compared with the comparative example 1, the fungus growing time and the fungus growing time are both reduced, the cultivation period is shortened by 5 days, the fungus sack pollution rate is reduced by 0.25%, the average single-sack yield is not high in comparison, but the difference is not obvious, the ear size is uniform, and the taste is more chewy.
The method utilizes agricultural wastes such as corncobs and crop straws to replace part of sawdust to cultivate the black fungus, so that the sources of the cultivation raw materials of the black fungus are wider and more convenient, and the raw material cost of the black fungus can be greatly reduced.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The culture medium for cultivating the black fungus is characterized by being prepared from solid raw materials and water according to the weight ratio of 1:1.1-1.4, and the pH value of the culture medium is 7-7.5, wherein the solid raw materials comprise the following raw materials in parts by weight: 37-49 parts of broad-leaved tree sawdust, 28-35 parts of corncobs, 10-15 parts of crop straws, 9-10 parts of wheat bran, 1.7-2.3 parts of bean flour, 0.5-1 part of gypsum powder and 0.5-1 part of lime powder.
2. The culture medium for cultivating black fungus according to claim 1, wherein the culture medium is prepared from solid raw materials and water according to a weight ratio of 1:1.3, wherein the solid raw materials comprise the following raw materials in parts by weight: 45 parts of broad-leaved tree sawdust, 30 parts of corncobs, 12 parts of crop straws, 9 parts of wheat bran, 2 parts of soybean powder, 1 part of gypsum powder and 1 part of lime powder.
3. The culture medium for cultivating black fungus according to claim 1 or 2, wherein the crop straw is one or more of corn straw, beanstalk, sunflower stalk, wheat straw and rice straw.
4. A preparation method of a culture medium for cultivating black fungus is characterized by comprising the following steps:
(1) crushing: respectively crushing broad-leaved tree sawdust, corncobs and crop straws into particles with the diameter less than 0.3 cm;
(2) weighing: weighing 37-49 parts of broad-leaved tree sawdust, 28-35 parts of corncobs, 10-15 parts of crop straws, 9-10 parts of wheat bran, 1.7-2.3 parts of bean flour, 0.5-1 part of gypsum powder and 0.5-1 part of lime powder according to the weight ratio, uniformly mixing to obtain a solid raw material, putting the solid raw material into a stirrer, adding water into the stirrer according to the weight ratio of the solid raw material to the water of 1:1.1-1.4, controlling the pH value of a mixture of the solid raw material and the water to be 7-7.5, and uniformly stirring to obtain a mixed material;
(3) bagging: bagging the mixed material in the step (2), and using a high-temperature-resistant and high-pressure-resistant polypropylene corner folding plastic bag with the specification of 17cm multiplied by 36cm multiplied by 0.04cm or 17cm multiplied by 38cm multiplied by 0.04 cm;
(4) screwing the opening: preparing a plastic core rod in advance by adopting a professional socket machine, placing the material bag loaded in the step (3) on a rotary table, putting a central drill in the center of the material bag by a right hand, binding the material ports together by the left hand by taking the central drill as a rotary shaft, treading a rotator by the right hand to rotate the rotary table, simultaneously, forcibly plugging the material ports into the center of the material bag by the right hand downwards, treading down the left foot, lifting the right foot, pulling out the central drill while reversing the rotary table, inserting a bacterium stick, and plugging a sponge cover body at a socket;
(5) and (3) sterilization: sterilizing the material bag screwed in the step (4), wherein the sterilization is mainly used for killing harmful bacteria and other microorganisms including propagules and spores in the culture material and promoting partial decomposition of organic substances of the culture material;
(6) and (3) cooling: after sterilization, the fungus bags are taken out from the sterilization pot, cooled to below 30 ℃, and then transferred into an inoculation room for inoculation and culture under aseptic conditions.
5. The method for preparing a culture medium for cultivating black fungus according to claim 4, wherein during the sterilization in the step (5), either one of atmospheric steam sterilization and high-pressure steam sterilization can be adopted;
wherein, the normal pressure steam sterilization adopts a normal pressure sterilization pot, the material bag is put into the sterilization pot and then is fiercely burnt by strong fire, the pot is required to be boiled within 3 to 4 hours, and the sterilization is thoroughly kept for 10 to 12 hours;
the high-pressure steam sterilization adopts a high-pressure steam sterilization pot, cold air is required to be discharged firstly during high-pressure sterilization, and when the pressure reaches 0.153MPa and the steam temperature is 128.1 ℃, the steam is kept for 2.5 to 3 hours, so that the aim of sterilization can be fulfilled.
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|---|---|---|---|---|
| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
| CN113767812B (en) * | 2021-09-23 | 2023-04-25 | 山西农业大学山西功能食品研究院 | Ganoderma lucidum strain and method for culturing red-meat ganoderma lucidum fruiting body |
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