CN112689757A - 使用crof和机器学习的基于图像测定的系统和方法 - Google Patents

使用crof和机器学习的基于图像测定的系统和方法 Download PDF

Info

Publication number
CN112689757A
CN112689757A CN201880083333.1A CN201880083333A CN112689757A CN 112689757 A CN112689757 A CN 112689757A CN 201880083333 A CN201880083333 A CN 201880083333A CN 112689757 A CN112689757 A CN 112689757A
Authority
CN
China
Prior art keywords
sample
analyte
image
plates
certain embodiments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880083333.1A
Other languages
English (en)
Chinese (zh)
Inventor
斯蒂芬·Y·周
丁惟
周芜
田军
张越成
吴明权
李力
迈克尔·莱昂利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Yisheng Biotechnology Co ltd
Yewei Co ltd
Original Assignee
Essenlix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Essenlix Corp filed Critical Essenlix Corp
Publication of CN112689757A publication Critical patent/CN112689757A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/21—Design or setup of recognition systems or techniques; Extraction of features in feature space; Blind source separation
    • G06F18/214—Generating training patterns; Bootstrap methods, e.g. bagging or boosting
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06M—COUNTING MECHANISMS; COUNTING OF OBJECTS NOT OTHERWISE PROVIDED FOR
    • G06M11/00—Counting of objects distributed at random, e.g. on a surface
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/075—Investigating concentration of particle suspensions by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/018—Platelets

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Multimedia (AREA)
  • Data Mining & Analysis (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Evolutionary Computation (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Artificial Intelligence (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Image Analysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201880083333.1A 2017-10-26 2018-10-26 使用crof和机器学习的基于图像测定的系统和方法 Pending CN112689757A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762577481P 2017-10-26 2017-10-26
US62/577,481 2017-10-26
PCT/US2018/057877 WO2019084517A1 (en) 2017-10-26 2018-10-26 SYSTEM AND METHODS FOR IMAGE-BASED ANALYSIS USING MACHINE LEARNING AND CROF

Publications (1)

Publication Number Publication Date
CN112689757A true CN112689757A (zh) 2021-04-20

Family

ID=66246761

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880083333.1A Pending CN112689757A (zh) 2017-10-26 2018-10-26 使用crof和机器学习的基于图像测定的系统和方法

Country Status (5)

Country Link
US (1) US20200256856A1 (https=)
EP (1) EP3701260A4 (https=)
JP (1) JP2021500901A (https=)
CN (1) CN112689757A (https=)
WO (1) WO2019084517A1 (https=)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3700419A4 (en) * 2017-10-26 2021-11-10 Essenlix Corporation QUICK MEASUREMENT OF PLATES
WO2019173283A1 (en) 2018-03-05 2019-09-12 Marquette University Method and apparatus for non-invasive hemoglobin level prediction
EP3575813B1 (en) * 2018-05-30 2022-06-29 Siemens Healthcare GmbH Quantitative mapping of a magnetic resonance imaging parameter by data-driven signal-model learning
EP3837543A4 (en) * 2018-08-16 2022-11-16 Essenlix Corporation CELL ANALYSIS IN BODY FLUID, ESPECIALLY BLOOD
US11068694B2 (en) * 2019-01-23 2021-07-20 Molecular Devices, Llc Image analysis system and method of using the image analysis system
WO2020189219A1 (ja) * 2019-03-19 2020-09-24 富士フイルム株式会社 情報処理装置、細胞培養システム、情報処理方法、及び情報処理プログラム
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11593649B2 (en) * 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
CN110223279B (zh) * 2019-05-31 2021-10-08 上海商汤智能科技有限公司 一种图像处理方法和装置、电子设备
CN113906438B (zh) * 2019-06-03 2025-08-05 三星电子株式会社 用于对象标识的电子设备及其控制方法
WO2021011944A2 (en) * 2019-07-18 2021-01-21 Essenlix Corporation Imaging based homogeneous assay
GB2583149B (en) * 2019-07-19 2021-03-17 Forsite Diagnostics Ltd Assay reading method
EP4065683A1 (en) * 2019-11-27 2022-10-05 Corning Incorporated Sensing vessels for cell cultures
US12361560B2 (en) * 2019-11-28 2025-07-15 Beijing Xiaoying Technology Co., Ltd. Blood smear full-view intelligent analysis method, and blood cell segmentation model and recognition model construction method
CN121034400A (zh) 2020-02-20 2025-11-28 因美纳有限公司 基于人工智能的多对多碱基判读
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
US12591780B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Data compression for artificial intelligence-based base calling
US12592298B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Hardware execution and acceleration of artificial intelligence-based base caller
US12055542B2 (en) 2020-05-29 2024-08-06 Quidel Corporation System and methods for remote assessment of a sample assay for disease diagnostics
CN115836327A (zh) * 2020-05-29 2023-03-21 奎多公司 用于疾病诊断的样本测定的远程评估的系统和方法
WO2022081863A2 (en) 2020-10-16 2022-04-21 Life Technologies Corporation Systems and methods for autofocus and automated cell count using artificial intelligence
US12525320B2 (en) 2021-03-16 2026-01-13 Illumina, Inc. Neural network parameter quantization for base calling
WO2022204611A1 (en) * 2021-03-26 2022-09-29 Essenlix Corporation Cells and other bio-entity analysis and counting
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
CN118140146A (zh) * 2021-07-12 2024-06-04 Essenlix 公司 多样品分析装置
CN114266731B (zh) * 2021-11-29 2025-05-27 安徽中科本元信息科技有限公司 一种融合牙弓二维图像的咬合检测分析系统及实现方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105027165A (zh) * 2013-03-15 2015-11-04 文塔纳医疗系统公司 用于数字完整载片的自动化评分的基于组织对象的机器学习系统
WO2017048871A1 (en) * 2015-09-14 2017-03-23 Essenlix Corp. Device and system for analyzing a sample, particularly blood, as well as methods of using the same
US20170169567A1 (en) * 2014-05-23 2017-06-15 Ventana Medical Systems, Inc. Systems and methods for detection of structures and/or patterns in images

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5656499A (en) * 1994-08-01 1997-08-12 Abbott Laboratories Method for performing automated hematology and cytometry analysis
CN101578520B (zh) * 2006-10-18 2015-09-16 哈佛学院院长等 基于形成图案的多孔介质的横向流动和穿过生物测定装置、及其制备方法和使用方法
SG174617A1 (en) * 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
CN102762713B (zh) * 2010-01-20 2016-04-27 Emd密理博公司 细胞图像捕捉和远程监控系统
CN104718552B (zh) * 2012-09-13 2017-11-28 多伦多大学理事会 用于胎儿和母体红细胞计数的系统和方法
US10176363B2 (en) * 2014-06-16 2019-01-08 Siemens Healthcare Diagnostics Inc. Analyzing digital holographic microscopy data for hematology applications
US9836839B2 (en) * 2015-05-28 2017-12-05 Tokitae Llc Image analysis systems and related methods
CN113376364B (zh) * 2015-08-10 2025-02-25 上海宜晟生物科技有限公司 步骤简化、小样品、快速、易使用的生物/化学分析装置和方法
CN105260730A (zh) * 2015-11-24 2016-01-20 严媚 基于机器学习的接触式成像微流细胞计数器及其图像处理方法
JP6750033B2 (ja) * 2016-04-08 2020-09-02 アレンティック マイクロサイエンス インコーポレイテッド 顕微鏡検査のための試料処理
CN106202997B (zh) * 2016-06-29 2018-10-30 四川大学 一种基于深度学习的细胞分裂检测方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105027165A (zh) * 2013-03-15 2015-11-04 文塔纳医疗系统公司 用于数字完整载片的自动化评分的基于组织对象的机器学习系统
US20170169567A1 (en) * 2014-05-23 2017-06-15 Ventana Medical Systems, Inc. Systems and methods for detection of structures and/or patterns in images
WO2017048871A1 (en) * 2015-09-14 2017-03-23 Essenlix Corp. Device and system for analyzing a sample, particularly blood, as well as methods of using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MANUEL G. FORERO 等: "Image Processing Methods for Automatic Cell Counting In Vivo or In Situ Using 3D Confocal Microscopy", 《ADVANCED BIOMEDICAL ENGINEERING》, pages 183 - 204 *
WEIDI XIE 等: "Microscopy cell counting and detection with fully convolutional regression networks", 《COMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL NGINEERING: IMAGING & VISUALIZATION》, pages 1 - 10 *

Also Published As

Publication number Publication date
EP3701260A4 (en) 2021-10-27
WO2019084517A1 (en) 2019-05-02
JP2021500901A (ja) 2021-01-14
EP3701260A1 (en) 2020-09-02
US20200256856A1 (en) 2020-08-13

Similar Documents

Publication Publication Date Title
CN112689757A (zh) 使用crof和机器学习的基于图像测定的系统和方法
US11674883B2 (en) Image-based assay performance improvement
US12248533B2 (en) Efficient training and accuracy improvement of imaging based assay
US11842483B2 (en) Systems for cell shape estimation
CN113227755B (zh) 测定准确度改善
Doan et al. Leveraging machine vision in cell-based diagnostics to do more with less
CN114341619B (zh) 测定准确度以及可靠性提高
CN113227754B (zh) 使用智能监测结构的基于图像的测定
Carrio et al. Automated low-cost smartphone-based lateral flow saliva test reader for drugs-of-abuse detection
US11609224B2 (en) Devices and methods for white blood cell analyses
US9697582B2 (en) Methods for obtaining and analyzing images
CN112218577A (zh) 用于组织和细胞染色的装置和方法
WO2018148471A2 (en) Optics, device, and system for assaying
EP3723611A2 (en) Improved optical transmission sample holder and analysis, particularly for hemoglobin
Lee et al. Machine learning-aided three-dimensional morphological quantification of angiogenic vasculature in the multiculture microfluidic platform
US20240085304A1 (en) Imaging Based Assay Accuracy Improvement Through Guided Training
Lee et al. Differential cell counts using center-point networks achieves human-level accuracy and efficiency over segmentation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20231116

Address after: 200245 Room 409, 4th Floor, Building 2, No. 1508 Kunyang Road, Minhang District, Shanghai

Applicant after: Shanghai Yisheng Biotechnology Co.,Ltd.

Address before: Unit B, 14th Floor, Wing Cheung Commercial Building, 19-25 Suhang Street, Sheung Wan, Hong Kong, China

Applicant before: Yewei Co.,Ltd.

Effective date of registration: 20231116

Address after: Unit B, 14th Floor, Wing Cheung Commercial Building, 19-25 Suhang Street, Sheung Wan, Hong Kong, China

Applicant after: Yewei Co.,Ltd.

Address before: Zhang Keshen, New Jersey, USA

Applicant before: Essenlix