CN112680362A - Fungus for promoting compost maturity, screening method and application - Google Patents
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Abstract
The invention discloses a fungus for promoting compost maturity, a screening method and application, and relates to the technical field of biology, wherein the preservation number is as follows: CGMCC No.21024, a method for screening fungi for promoting compost maturity, comprising the following steps: s1: adding the decomposed chicken manure and the sterilized water into a triangular flask according to the weight part ratio of 1:9, sucking supernatant, and diluting the supernatant into 10 by using sterile water‑5A concentration gradient of bacterial suspension; s2: respectively sucking 0.1mL of bacterial suspensions with different concentrations, coating the bacterial suspensions on a martin culture medium for culturing for 7d, wherein the culturing temperature is 25 ℃, and selecting fungi with different forms to perform point grafting on the improved martin culture medium for purification; s3: preparing purified fungus bacterial suspension by receiving the improved martin culture medium, and culturing in a constant-temperature incubator at 25 ℃ for 7 d; s4: mixing a chicken manure sample and deionized water according to the weight part ratio of 1:9 mixing in a triangular flask, by counting the seedsAnd screening fungi for promoting compost maturity by using the germination index. Can accelerate the decomposition process and shorten the composting period.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a fungus for promoting compost maturity, a screening method and application.
Background
Aerobic composting is a biochemical process for degrading organic matters by the action of obligate and facultative aerobic microorganisms, organic materials to be composted are mixed with auxiliary materials according to a certain proportion, and microorganisms propagate and degrade the organic matters under the conditions of proper moisture and ventilation, so that high temperature is generated, pathogenic bacteria and weed seeds in the organic matters are killed, and the organic matters are stabilized. Aerobic composting is an important way for recycling and harmless treatment of livestock and poultry wastes, easily degradable organic matters are decomposed by microorganisms in the composting process and then absorbed and converted into self cell substances, and the organic matters which are not easily degradable are mineralized and then humified, wherein the mineralization and humification mainly correspond to two stages of primary fermentation and secondary fermentation of the composting. The primary fermentation refers to a compost heating period and a high-temperature period, then the compost enters a secondary fermentation stage along with the continuous reduction of the temperature, and microorganisms further decompose the residual organic matters in the secondary fermentation stage and humify the formed minerals.
Researches show that the livestock and poultry manure production in China is increased and slowed, the total quantity gradually tends to be stable, the current annual production of breeding wastes can reach 38 hundred million tons, but the comprehensive utilization rate is less than 60%. The wastes which are not recycled or harmlessly treated seriously pollute the environment and are harmful to human and animal health to a certain extent. Therefore, how to improve the comprehensive utilization rate of livestock and poultry breeding waste becomes a problem to be solved urgently at present. Aerobic composting is an important mode for harmless treatment and resource utilization of livestock and poultry wastes, but in actual production, the composting efficiency is severely limited by the problems of overlong composting period, limited field, insufficient fermentation and the like.
Inoculation of specific exogenous microorganisms is an effective method for accelerating the composting process and shortening the composting period, but most of exogenous microorganisms are used for promoting compost temperature rise and improving the efficiency and quality of primary fermentation, and microbial agents for promoting secondary fermentation of compost are lacking in the market at present.
Disclosure of Invention
In order to solve the technical problems, the invention provides a fungus for promoting compost maturity, a screening method and application, and the fungus for promoting compost maturity is screened out, so that the composting process can be accelerated, and the composting period can be shortened.
The invention is realized by the following technical scheme:
the fungus for promoting compost maturity is preserved in China general microbiological culture Collection center on 21 days 12 months 2020 at the preservation address: the preservation number of the Xilu No. 1 Hospital No. 3 of the Chaojing area facing the Yang is as follows: CGMCC No.21024, the biological classification name is: alternaria sp. The preservation number is: CGMCC No. 21024.
The screening method of the fungi for promoting compost maturity comprises the following steps:
s1: adding decomposed chicken manure and sterilized water into a triangular flask according to the weight ratio of 1:9, standing at room temperature in a shaking table, shaking for 30min, taking out, standing for 5min, sucking supernatant, and diluting with sterile water to 10%-5A concentration gradient of bacterial suspension;
s2: respectively sucking 0.1mL of bacterial suspensions with different concentrations, coating the bacterial suspensions on a martin culture medium for culturing for 7d, wherein the culturing temperature is 25 ℃, selecting fungi with different forms, carrying out point grafting on the improved martin culture medium for purification, and obtaining purified fungi after purification;
s3: respectively inoculating purified fungi to an improved Martin culture medium to prepare a purified fungi suspension, inoculating the purified fungi suspension into a triangular flask containing 50g of chicken manure according to the inoculation amount of 10%, and placing the triangular flask in a constant-temperature incubator at 25 ℃ for culturing for 7d to obtain a chicken manure sample;
s4: mixing a chicken manure sample and deionized water according to the weight part ratio of 1:9 mixing in a triangular flask, placing in a shaking table, shaking and leaching for 1h, filtering, sucking 10mL of filtrate, adding into a culture dish paved with filter paper, dibbling wheat seeds in the culture dish, and screening fungi for promoting compost maturity by calculating the germination index of the seeds.
Further, the preparation method of the martin culture medium in the step S2 includes the following steps:
a1: mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 18 parts of agar and 1000 parts of distilled water in parts by weight to obtain a mixed solution;
a2: and (3) sterilizing the mixed solution, pouring the sterilized mixed solution into a flat plate, and adding a 1% bengal aqueous solution and a 1% streptomycin solution to finally obtain the martin culture medium.
Further, the mixed solution, the 1% aqueous solution of montage-malachite and the 1% streptomycin solution are 1044.5: 3.3:3.
Further, the preparation method of the improved martin medium in the step S2 includes the following steps:
b1, mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 2 parts of yeast powder, 18 parts of agar and 1000 parts of distilled water according to parts by weight to obtain a mixed solution;
b2, adjusting the pH of the mixed solution to 6.2-6.6.
The application of the fungus for promoting compost maturity is to apply the fungus for promoting compost maturity to compost secondary fermentation.
Compared with the prior art, the invention has the following advantages and beneficial effects: the fungus for promoting compost maturity can greatly improve the effect of secondary fermentation of compost.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a diagram illustrating the effect of fungus on promoting compost maturity;
FIG. 2 is a graph showing the temperature change during secondary composting;
FIG. 3 is the change in EC during secondary composting;
FIG. 4 is the GI change during secondary composting;
FIG. 5 shows the total humic acid, fulvic acid content and PHA content variation during secondary composting;
FIG. 6 is a variation of F3 windrow during secondary composting;
FIG. 7 shows the colony morphology of FCM3 on different days of culture;
FIG. 8 shows PCR electrophoresis.
Detailed Description
The present invention will be further described with reference to the following examples. The following examples are only a few specific examples of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by the design concept should fall within the scope of infringing on the protection scope of the present invention.
The methods in the following examples are conventional methods unless otherwise specified.
The percentages in the following examples are by mass unless otherwise specified.
Examples
In one embodiment, a compost maturity promoting fungus of deposit number: CGMCC No. 21024.
In one embodiment, a method of screening for fungi that promote compost maturity, comprising the steps of:
s1: adding decomposed chicken manure and sterilized water into a triangular flask according to the weight ratio of 1:9, standing at room temperature in a shaking table, shaking for 30min, taking out, standing for 5min, sucking supernatant, and diluting with sterile water to 10%-5A concentration gradient of bacterial suspension;
s2: respectively sucking 0.1mL of bacterial suspensions with different concentrations, coating the bacterial suspensions on a martin culture medium for culturing for 7d, wherein the culturing temperature is 25 ℃, selecting fungi with different forms, carrying out point grafting on the improved martin culture medium for purification, and obtaining purified fungi after purification;
s3: respectively inoculating purified fungi to an improved Martin culture medium to prepare a purified fungi suspension, inoculating the purified fungi suspension into a triangular flask containing 50g of chicken manure according to the inoculation amount of 10%, and placing the triangular flask in a constant-temperature incubator at 25 ℃ for culturing for 7d to obtain a chicken manure sample;
s4: mixing a chicken manure sample and deionized water according to the weight part ratio of 1:9 mixing in a triangular flask, placing in a shaking table, shaking and leaching for 1h, filtering, sucking 10mL of filtrate, adding into a culture dish paved with filter paper, dibbling wheat seeds in the culture dish, and screening fungi for promoting compost maturity by calculating the germination index of the seeds.
In one embodiment, the preparation method of the martin culture medium in the step S2 includes the following steps:
a1: mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 18 parts of agar and 1000 parts of distilled water in parts by weight to obtain a mixed solution;
a2: and (3) sterilizing the mixed solution, pouring the sterilized mixed solution into a flat plate, and adding a 1% bengal aqueous solution and a 1% streptomycin solution to finally obtain the martin culture medium.
In one embodiment, the mixture, 1% aqueous solution of mengladesh, 1% streptomycin solution, in parts by weight, is 1044.5: 3.3:3.
In one embodiment, the preparation method of the modified martin culture medium in the step S2 includes the following steps:
b1, mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 2 parts of yeast powder, 18 parts of agar and 1000 parts of distilled water according to parts by weight to obtain a mixed solution;
b2, adjusting the pH of the mixed solution to 6.2-6.6.
In one embodiment, the application of the compost-decomposing promoting fungi applies the compost-decomposing promoting fungi to the compost secondary fermentation.
Examples of the experiments
1. Selection of strains
Respectively inoculating the purified fungi in the embodiment to a liquid improved Martin culture medium to prepare a bacterial suspension, inoculating the bacterial suspension into a triangular flask containing 50g of chicken manure according to the inoculation amount of 10%, and culturing in a constant-temperature incubator at 25 ℃ for 7d to obtain a chicken manure sample. Dividing the chicken manure into five groups, weighing more than 10g of chicken manure samples in each group, putting the chicken manure samples into a triangular flask filled with 90mL of deionized water, placing the triangular flask in a shaking table, shaking and leaching for 1h, filtering to obtain leaching liquor, adding 10mL of leaching liquor into culture dishes paved with filter paper, dibbling 10 full wheat seeds in each culture dish, taking distilled water to replace the leaching liquor as a blank control, culturing for 72h at 25 ℃ in a constant-temperature incubator, measuring the germination rate and the root length, and calculating the Germination Index (GI) of the seeds.
GI can directly reflect the phytotoxicity of the stockpile and can judge the rotten degree of the stockpile at the same time. 5 strains of fungi are obtained by separation and purification, and are respectively numbered as FCM1-FCM 5. FIG. 1 shows the results of the test of the decomposing effect of 5 strains of fungi. The results show that the GI values of the fungi FCM1 and FCM3 are 12.3% and 12.5% higher than that of CK respectively, and the decomposition of the chicken manure is remarkably promoted (P is less than 0.05). Therefore, the fungi FCM1 and FCM3 were selected for compost secondary fermentation application studies.
2. Compost secondary fermentation test
Test raw materials: the secondary fermentation test takes the material subjected to primary fermentation harmlessness as a test raw material, the material subjected to primary fermentation harmlessness is a compost obtained by mixing chicken manure and edible fungus residues for 10 days, the primary fermentation condition is that the high temperature is more than 50 ℃ and lasts for 5 days, and the highest temperature is 60 ℃.
Secondary fermentation experiment: the experiment was set up in 3 groups, control CK: adding 1% sterile liquid culture medium into the test raw materials; f1: adding 1% FCM1 bacterial liquid into the test raw materials; f3: the test material was added with 1% FCM3 bacterial solution. 1 percent refers to the ratio of the volume of the bacterial liquid to the mass of the dry matter, and the unit is L/kg), and 60kg of the experimental raw materials and the bacterial liquid are accurately weighed and put into a foam box for carrying out the secondary fermentation test of the compost after being fully mixed. The test adopts manual pile turning, pile turning and sampling are carried out at 1 st, 7 th, 14 th, 21 th, 28 th, 35 th, 45 th and 60 th days, and the temperature, EC, seed germination index, total humic acid, humic acid and fulvic acid content are measured.
The experimental results are as follows:
1) influence on compost temperature
As shown in FIG. 2, the secondary fermentation raw material still contains undegraded material, so that each treatment in the test is subjected to the processes of temperature rise and temperature drop, and finally tends to be consistent with the change of the environmental temperature. CK. F1 and F3 began heating at compost nos. 19, 25 and 11d, respectively, until compost nos. 27, 35 and 17d reached the highest temperature. The highest temperature of CK in the whole secondary fermentation process is only 50 ℃, and the treatment of adding the fungus FCM3 has the advantages of fast temperature rise and longest duration (13d) at high temperature (>50 ℃), which is 12d more than that of CK treatment, and shows that the addition of FCM3 can promote the fast decomposition of stockpile and is more favorable for the harmlessness of livestock and poultry manure.
2) Effect on compost EC
As shown in fig. 3, EC of 14-21d, F3 was significantly lower than other treatments, indicating that addition of FMC3 promoted the humification process at this stage, resulting in a decrease in soluble salt concentration, more favorable to plant growth.
3) Influence on seed germination index
As shown in fig. 4, the GI of F3 was significantly higher than the other treatments and reached complete composting at 35d, 25d and 10d earlier than the CK, F1 treatments, respectively, indicating that the fungus FCM3 was effective in promoting compost maturity and rendering the compost harmless.
4) Effect on Total humic, humic and Fulic acids
The total humic, humic and fulvic acid content changes for each group are shown in figure 5. As is clear from FIG. 5A, the total humic acid content of each treatment was maintained at about 180 g.kg-1 at the end of composting. Humic acid is a substance with high molecular weight and high stability in humic acid, contains various functional groups such as carboxyl, phenolic hydroxyl and the like, and is also a substance which is most beneficial to soil remediation. As is clear from FIG. 5B, the content of CK humic acid at the end of composting was reduced by 13.63 g/kg as compared with the initial stage-1However, F1 and F3 were increased by 3.99 and 7.65 g.kg, respectively-1This indicates that the addition of the fungus favours the production of humic acid and that the fungus FCM3 is more effective. As can be seen from FIG. 5C, the fulvic acid contents of CK, F1 and F3 at the end of composting were 65.62, 58.81 and 45.17 g.kg-1The fulvic acid content of the F3 treatment was significantly lower than the other treatments (P)<0.05). PHA (humic acid/total humic acid) is an important parameter for evaluating the humification degree of compost, the higher the value of PHA is, the higher the oxidation degree and aromatization degree of compost is, and the compost product of PHA can be used in soil to promote the growth of plant root, and can also enhance the respiration and photosynthesis of plant. As can be seen in FIG. 5D, PHA of F3 rose rapidly at 14-21D compared to CK and F1, and was higher after 21D than the other treatments; at the end of the test, it was 10.99% higher than the non-CK treatment, indicating that F3 is significantly better in terms of humification than the other treatments.
5) Change in windrow after addition of FCM3
As can be seen from FIG. 6, a small amount of white fungus (7d) appeared on the surface of the heap, while CK did not change significantly, and this fungus was assumed to be FCM 3; as FCM3 grows, the surface of the pack spreads over the white hyphae (14d), and the temperature of the pack is continuously increased, which shows that the temperature of the pack is effectively increased by the growth of FCM 3; however, FCM3 largely hibernates or dies when the temperature is above 50 ℃ (21 d); at 28d, a small amount of white hypha is remained on the surface layer of the compost, and the compost is nearly decomposed (GI (total mark) is 77.14 percent); after 35d, the white hyphae disappear, the temperature of the compost is continuously reduced and approaches to the ambient temperature, and the compost is completely decomposed.
In conclusion, the fungus FCM3 can obviously promote the secondary fermentation and decomposition of the compost and improve the quality of the compost, and has better application prospect.
3. Identification of strains
3.1 morphological characterization of FCM3
FCM3 was inoculated in modified Martin medium, cultured for seven days in a constant temperature shaking incubator at a rotation speed of 150rpm and a temperature of 25 ℃ and observed for colony morphology, and FCM3 microscopic morphology was observed on day 7.
As shown in FIG. 7, FCM3 showed a colony diameter of 70mm and a colony flocculent after 7 days of culture on modified Martin medium at 25 ℃. The colony was white at the initial stage, darkened after aging, and brown at the back. The sterile hyphae of the FCM3 crawl, separate and singly grow or cluster conidium peduncles; most are not branched, are short and hardly have difference with vegetative hyphae; the conidiophores are inverted rod-shaped, the top of the conidiophores is extended into a beak shape, the conidiophores are light brown, the conidiophores are separated by wall bricks, the conidiophores are dark brown, and a plurality of conidiophores are chained.
3.2 molecular characterization of FCM3
1) Selection of kit
Selecting a DNAsecure novel plant genome DNA extraction kit, which comprises the following components: buffer LP1(Buffer LP1), Buffer LP2 (Buffer LP2), Buffer LP3(Buffer LP3), rinsing PW (Buffer PW), elution Buffer TE (Buffer TE), RNase A (10mg/ml), adsorption column CB3(Spin Columns CB3), and Collection tube (2ml) (Collection Tubes 2 ml).
2) Extraction of fungal genomic DNA
(1) Treating the materials: from the above 3.1FCM3 morphometric assay, FCM3 was cultured until the third day, and a sample of hyphae was scraped and ground thoroughly with liquid nitrogen. 400. mu.l of buffer LP1 and 6. mu.l of RNaseA (10mg/ml) were added, vortexed for 1min, and allowed to stand at room temperature for 10 min.
(2) Add 130. mu.l of buffer LP2, mix well and vortex for 1 min.
(3) Centrifuge at 12000rpm for 5min and transfer the supernatant to a new centrifuge tube.
(4) After adding 1.5 times the volume of the buffer LP3 (before use, check if absolute ethanol has been added), it is immediately mixed thoroughly for 15sec with shaking, at which time a flocculent precipitate may appear.
(5) Adding the solution and flocculent precipitate obtained in the previous step into an adsorption column CB3 (the adsorption column is put into a collecting pipe), centrifuging at 12000rpm for 30sec, pouring off waste liquid, and putting an adsorption column CB3 into the collecting pipe.
(6) To the adsorption column CB3, 600. mu.l of a rinsing solution PW (previously used, whether or not absolute ethanol was added) was added, and the mixture was centrifuged at 12000rpm for 30sec, and the waste liquid was discarded, and the adsorption column CB3 was put into a collection tube.
(7) And (5) repeating the operation step (6).
(8) The adsorption column CB3 was put back into the collection tube, centrifuged at 12000rpm for 2min, and the waste liquid was discarded. The adsorption column CB3 was left at room temperature for several minutes to completely dry the residual rinse solution in the adsorption material.
(9) Transferring the adsorption column CB3 into a clean centrifuge tube, suspending and dripping 50-200 μ l of elution buffer TE into the middle part of the adsorption membrane, standing at room temperature for 2-5min, centrifuging at 12000rpm for 2min, and collecting the solution into the centrifuge tube.
3) PCR amplification
And (3) carrying out ITS sequence amplification on the extracted genome DNA, and carrying out PCR reaction primers for amplification: forward primer ITS1 (5'-TCCGTAGGTGAACCTGCGG-3'); the reverse primer ITS4 (5'-TCCTCCGCTTATTGATATGC-3'). The PCR amplification system is as follows: mu.l of genomic DNA (20 ng/. mu.l), 5.0. mu.l of 10 XBuffer (containing 2.5mM Mg2+), 1.0. mu.l of Taq polymerase (5 u/. mu.L), 1.0. mu.l dNTP (10mM), 1.5. mu.l ITS1 primer (10. mu.M), 1.5. mu.l ITS4 primer (10. mu.M), and 39. mu.l of sterile deionized water was added to form a 50. mu.l amplification system. Amplification conditions: pre-denaturation at 95 ℃ for 5 min; then denaturation at 95 ℃ for 30s, annealing at 58 ℃ for 30s, extension at 72 ℃ for 7min, and 35 cycles; 3. mu.l of the PCR product was subjected to 1% agarose gel electrophoresis to confirm the PCR-amplified fragment. As shown in FIG. 8, the results of the electrophoretogram of the PCR products showed that the fragments were successfully amplified.
4) Sequencing and alignment results
The FCM3 sequence obtained by sequencing is shown in a sequence table, and after BLAST alignment in NCBI database, the fungus FCM3 has the highest homology with Alternaria sp.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Sequence listing
<110> institute of agricultural resources and agricultural regionalism of Chinese academy of agricultural sciences
<120> fungus for promoting compost maturity, screening method and application
<130> YB216211
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 536
<212> DNA
<213> Alternaria alternata (Alternaria sp.)
<400> 1
atcctacctg atccgaggtc aaagttgaaa aaaaggctta atggatgcta gacctttgct 60
gatagagagt gcgacttgtg ctgcgctccg aaaccagtag gccggctgcc aattacttta 120
aggcgagtct ccagcaaagc tagagacaag acgcccaaca ccaagcaaag cttgagggta 180
caaatgacgc tcgaacaggc atgccctttg gaataccaaa gggcgcaatg tgcgttcaaa 240
gattcgatga ttcactgaat tctgcaattc acactactta tcgcatttcg ctgcgttctt 300
catcgatgcc agaaccaaga gatccgttgt tgaaagttgt aattattaat ttgttactga 360
cgctgattgc aattacaaaa ggtttatgtt tgtcctagtg gtgggcgaac ccaccaagga 420
aacaagaagt acgcaaaaga caagggtgaa taattcagca aggctgtaac cccgagaggt 480
tccagcccgc cttcatattt gtgtaatgat ccctccgcag gcacccccta cggaaa 536
Claims (6)
1. A compost maturity promoting fungus, the preservation number of which is: CGMCC No. 21024.
2. A method of screening for compost maturity promoting fungi of claim 1 comprising the steps of:
s1: adding decomposed chicken manure and sterilized water into a triangular flask according to the weight ratio of 1:9, standing at room temperature in a shaking table, shaking for 30min, taking out, standing for 5min, sucking supernatant, and diluting with sterile water to 10%-5A concentration gradient of bacterial suspension;
s2: respectively sucking 0.1mL of bacterial suspensions with different concentrations, coating the bacterial suspensions on a martin culture medium for culturing for 7d, wherein the culturing temperature is 25 ℃, selecting fungi with different forms, carrying out point grafting on the improved martin culture medium for purification, and obtaining purified fungi after purification;
s3: respectively inoculating purified fungi to an improved Martin culture medium to prepare a purified fungi suspension, inoculating the purified fungi suspension into a triangular flask containing 50g of chicken manure according to the inoculation amount of 10%, and placing the triangular flask in a constant-temperature incubator at 25 ℃ for culturing for 7d to obtain a chicken manure sample;
s4: mixing a chicken manure sample and deionized water according to the weight part ratio of 1:9 mixing in a triangular flask, placing in a shaking table, shaking and leaching for 1h, filtering, sucking 10mL of filtrate, adding into a culture dish paved with filter paper, dibbling wheat seeds in the culture dish, and screening fungi for promoting compost maturity by calculating the germination index of the seeds.
3. The screening method according to claim 2, wherein the preparation method of the martin culture medium in the step S2 comprises the following steps:
a1: mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 18 parts of agar and 1000 parts of distilled water in parts by weight to obtain a mixed solution;
a2: and (3) sterilizing the mixed solution, pouring the sterilized mixed solution into a flat plate, and adding a 1% bengal aqueous solution and a 1% streptomycin solution to finally obtain the martin culture medium.
4. The screening method according to claim 3, wherein the mixed solution, the 1% aqueous solution of Bengal and the 1% streptomycin solution are 1044.5: 3.3:3.
5. The screening method according to claim 2, wherein the preparation method of the modified Martin medium in step S2 comprises the following steps:
b1, mixing 5 parts of peptone, 20 parts of glucose, 1 part of dipotassium hydrogen phosphate, 0.5 part of magnesium sulfate, 2 parts of yeast powder, 18 parts of agar and 1000 parts of distilled water according to parts by weight to obtain a mixed solution;
b2, adjusting the pH of the mixed solution to 6.2-6.6.
6. Use of a fungus for promoting compost maturity, characterized in that the fungus of claim 1 is used in compost secondary fermentation.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696393A (en) * | 2009-09-30 | 2010-04-21 | 江苏食品职业技术学院 | Compound bacterium agent and method for accelerating fast maturity by inoculating compound bacterium agent to compost |
| CN102010824A (en) * | 2010-09-07 | 2011-04-13 | 西北农林科技大学 | Microbial agent for promoting quick decomposition of agricultural wastes and preparation method |
| CN108795795A (en) * | 2018-05-04 | 2018-11-13 | 吉林农业大学 | A kind of lactobacteria-containing Thermal degradation stalk composite bacteria agent and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696393A (en) * | 2009-09-30 | 2010-04-21 | 江苏食品职业技术学院 | Compound bacterium agent and method for accelerating fast maturity by inoculating compound bacterium agent to compost |
| CN102010824A (en) * | 2010-09-07 | 2011-04-13 | 西北农林科技大学 | Microbial agent for promoting quick decomposition of agricultural wastes and preparation method |
| CN108795795A (en) * | 2018-05-04 | 2018-11-13 | 吉林农业大学 | A kind of lactobacteria-containing Thermal degradation stalk composite bacteria agent and its application |
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