CN112661872A - Method for extracting polysaccharide from solidago decurrens - Google Patents
Method for extracting polysaccharide from solidago decurrens Download PDFInfo
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- CN112661872A CN112661872A CN202110023598.6A CN202110023598A CN112661872A CN 112661872 A CN112661872 A CN 112661872A CN 202110023598 A CN202110023598 A CN 202110023598A CN 112661872 A CN112661872 A CN 112661872A
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Abstract
The invention discloses a method for extracting goldenrod herb polysaccharide, which comprises the following steps of firstly, selecting high-quality goldenrod herb plants, cleaning the goldenrod herb plants by using a cleaning device, fully drying the goldenrod herb plants by using a drying device after cleaning, crushing the goldenrod herb plants by using a crushing device after drying, weighing 500 g of goldenrod herb powder by using a weighing device, placing the goldenrod herb powder into a round-bottomed flask, extracting 2L of absolute ethyl alcohol by using a Soxhlet extractor for refluxing for 2h, filtering the reflowed goldenrod herb powder and the ethyl alcohol by using a filtering device after refluxing, filtering the mixture until the ethyl alcohol is volatilized to obtain filter residues for later use, weighing 2 g of goldenrod herb powder again, firstly, fixing the extraction time for 2h, and extracting for 1 time under the condition that the extraction temperature is 80 ℃. The invention provides reference for further development and utilization of the plant polysaccharide taking the solidago decurrens as the raw material in the field of traditional Chinese medicine, improves the extraction efficiency of the polysaccharide and meets the use requirements of people.
Description
Technical Field
The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting solidago decurrens polysaccharide.
Background
The solidago virgaurea is a species with a variety of types, the leaf shape and the inflorescence formula are greatly changed, the stem is upright and is usually thin and weak, the solidago virgaurea or a few of the solidago virgaurea grows in clusters, no branches are formed or branches are formed above the middle part, the stem leaves in the middle part are elliptical, long elliptical, oval or wide in a needle shape, the two surfaces, the veins and the leaf margins of the leaves have short and soft hairs or no hairs below the two surfaces, the veins and the leaf margins, and the solidago virginia is originally produced in China east China, the. The fertilizer is favored to grow in cool and humid climates, is cold-resistant and is suitable to be planted in fertile, loose, humus-rich and well-drained sandy soil. The Chinese medicinal herbs can be bred by adopting methods of seeds, plant division and the like, are full of herbs, are pungent in nature, bitter in taste and slightly warm in nature, and can dispel wind, clear heat, detoxify and clear swelling and the like.
The plant of the solidago virgaurea contains a large amount of polysaccharide, which is often required to be extracted in the research of the traditional Chinese medicine field, and the current commonly used plant polysaccharide extraction methods comprise a solvent extraction method, an ultrasonic-assisted solvent extraction method, a microwave-assisted solvent extraction method, an ultrasonic-microwave synergistic extraction method, an enzymatic hydrolysis method and a supercritical fluid extraction method.
The extraction method cannot optimize the hot water extraction conditions of the solidago virgaurea polysaccharide in the extraction process, cannot determine the optimal extraction process conditions of the solidago virgaurea polysaccharide, leads to low extraction efficiency, and obviously cannot meet the use requirements of people.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a method for extracting goldenrod herb polysaccharide.
The extraction method of the goldenrod herb polysaccharide provided by the invention comprises the following steps:
s1: firstly, selecting high-quality yellow flower plants, cleaning the yellow flower plants by using a cleaning device, fully drying the yellow flower plants by using a drying device after cleaning, and crushing the yellow flower plants by using a crushing device after drying;
s2: weighing 500 g of daylily powder by using a weighing device, placing the daylily powder into a round-bottom flask, and extracting 2L of absolute ethyl alcohol by using a Soxhlet extractor and refluxing for 2 h;
s3: after refluxing, filtering the refluxed daylily powder and ethanol by using a filtering device, and after filtering, waiting for the ethanol to volatilize to obtain filter residue for later use;
s4: weighing 2 g of daylily powder again, firstly, fixing the extraction time for 2h, extracting for 1 time at the extraction temperature of 80 ℃, respectively weighing the material-liquid ratios of 1:8, 1:11, 1:14, 1:17 and 1:20 to perform a Solidago virgaurea polysaccharide yield test, and drawing a test line graph of the material-liquid ratios and the yield;
s5: then, fixing the feed-liquid ratio at 1:17, extracting for 2h for 1 time, controlling the temperature at 60 ℃, 70 ℃, 80 ℃, 90 ℃ and 100 ℃ respectively to perform a goldenrod polysaccharide yield test, and drawing a test line graph of the temperature and the yield;
s6: finally, fixing the liquid-material ratio at 1:14, extracting at 80 ℃, extracting for 1 time, controlling the extraction time at 30min, 60min, 90min, 120min, 150min and 180min respectively to obtain the polysaccharide yield of the Solidago decurrens, and drawing test line graphs of the extraction time and the yield;
s7: through three line graphs, it can be easily found that when the optimal liquid-material ratio is 1: 14. when the temperature is 80 ℃ and 2 hours, the yield of the goldenrod herb polysaccharide is highest, the polysaccharide extraction is carried out by taking the polysaccharide as the extraction condition, the extracting solution is refluxed by 80 percent volume of absolute ethyl alcohol, then refrigerated and centrifuged by a refrigerated and centrifuged device, and the precipitate obtained by removing the supernatant is dried by a drying device, thus obtaining the crude goldenrod herb polysaccharide.
Preferably, the processing system comprises a cleaning device, a drying device, a crushing device, a weighing device, a filtering device, a freezing and centrifuging device and a drying device, wherein the crushing device is a Chinese herbal medicine crusher, and the Chinese herbal medicine crusher is FW-135 in model.
Preferably, the cleaning device is a numerical control ultrasonic cleaner, and the model of the numerical control ultrasonic cleaner is KQ-300 DB.
Preferably, the weighing device is an electronic analytical balance, and the electronic analytical balance is of the type FA-2004B.
Preferably, the refrigerated centrifuge is a benchtop high speed refrigerated centrifuge, and the benchtop high speed refrigerated centrifuge is model number H3-20 KR.
Preferably, the drying device is a glass instrument airflow dryer, and the model of the glass instrument airflow dryer is C-20.
The invention has the beneficial effects that: the experiment takes the solidago decurrens as a raw material, the solidago decurrens polysaccharide is extracted by a hot water extraction method, on the basis of a single-factor experiment, the hot water extraction condition of the solidago decurrens polysaccharide is optimized by a response surface method experimental design so as to determine the optimal extraction process condition of the solidago decurrens polysaccharide, provide reference for further development and utilization of the plant polysaccharide taking the solidago decurrens as the raw material in the field of traditional Chinese medicine, improve the extraction efficiency of the polysaccharide and meet the use requirements of people.
Drawings
FIG. 1 is a schematic diagram of the extraction process of the method for extracting Solidago decurrens polysaccharides according to the present invention;
FIG. 2 is a test line graph of the feed-liquid ratio and yield of the method for extracting Solidago decurrens polysaccharide provided by the present invention;
FIG. 3 is a test line graph of temperature and yield of the method for extracting Solidago decurrens polysaccharides according to the present invention;
FIG. 4 is a test line graph of the extraction time and yield of the method for extracting Solidago decurrens polysaccharides according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-4, the method for extracting the goldenrod herb polysaccharide comprises the following steps:
s1: firstly, selecting high-quality yellow flower plants, cleaning the yellow flower plants by using a cleaning device, fully drying the yellow flower plants by using a drying device after cleaning, and crushing the yellow flower plants by using a crushing device after drying;
s2: weighing 500 g of daylily powder by using a weighing device, placing the daylily powder into a round-bottom flask, and extracting 2L of absolute ethyl alcohol by using a Soxhlet extractor and refluxing for 2 h;
s3: after refluxing, filtering the refluxed daylily powder and ethanol by using a filtering device, and after filtering, waiting for the ethanol to volatilize to obtain filter residue for later use;
s4: weighing 2 g of daylily powder again, firstly, fixing the extraction time for 2h, extracting for 1 time at the extraction temperature of 80 ℃, respectively weighing the material-liquid ratios of 1:8, 1:11, 1:14, 1:17 and 1:20 to perform a Solidago virgaurea polysaccharide yield test, and drawing a test line graph of the material-liquid ratios and the yield;
s5: then, fixing the feed-liquid ratio at 1:17, extracting for 2h for 1 time, controlling the temperature at 60 ℃, 70 ℃, 80 ℃, 90 ℃ and 100 ℃ respectively to perform a goldenrod polysaccharide yield test, and drawing a test line graph of the temperature and the yield;
s6: finally, fixing the liquid-material ratio at 1:14, extracting at 80 ℃, extracting for 1 time, controlling the extraction time at 30min, 60min, 90min, 120min, 150min and 180min respectively to obtain the polysaccharide yield of the Solidago decurrens, and drawing test line graphs of the extraction time and the yield;
s7: through three line graphs, it can be easily found that when the optimal liquid-material ratio is 1: 14. when the temperature is 80 ℃ and 2 hours, the yield of the goldenrod herb polysaccharide is highest, the polysaccharide extraction is carried out by taking the polysaccharide as the extraction condition, the extracting solution is refluxed by 80 percent volume of absolute ethyl alcohol, then refrigerated and centrifuged by a refrigerated and centrifuged device, and the precipitate obtained by removing the supernatant is dried by a drying device, thus obtaining the crude goldenrod herb polysaccharide.
The processing system comprises a cleaning device, a drying device, a crushing device, a weighing device, a filtering device, a freezing and centrifuging device and a drying device, wherein the crushing device is a Chinese herbal medicine crusher, and the Chinese herbal medicine crusher is FW-135.
In the invention, the cleaning device is a numerical control ultrasonic cleaner, and the model of the numerical control ultrasonic cleaner is KQ-300 DB.
In the invention, the weighing device is an electronic analytical balance, and the model of the electronic analytical balance is FA-2004B.
In the invention, the freezing and centrifuging device is a table-type high-speed freezing centrifuge, and the model of the table-type high-speed freezing centrifuge is H3-20 KR.
In the invention, the drying device is a glass instrument airflow dryer, and the type of the glass instrument airflow dryer is C-20.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The extraction method of the solidago decurrens polysaccharide is characterized by comprising the following steps:
s1: firstly, selecting high-quality yellow flower plants, cleaning the yellow flower plants by using a cleaning device, fully drying the yellow flower plants by using a drying device after cleaning, and crushing the yellow flower plants by using a crushing device after drying;
s2: weighing 500 g of daylily powder by using a weighing device, placing the daylily powder into a round-bottom flask, and extracting 2L of absolute ethyl alcohol by using a Soxhlet extractor and refluxing for 2 h;
s3: after refluxing, filtering the refluxed daylily powder and ethanol by using a filtering device, and after filtering, waiting for the ethanol to volatilize to obtain filter residue for later use;
s4: weighing 2 g of daylily powder again, firstly, fixing the extraction time for 2h, extracting for 1 time at the extraction temperature of 80 ℃, respectively weighing the material-liquid ratios of 1:8, 1:11, 1:14, 1:17 and 1:20 to perform a Solidago virgaurea polysaccharide yield test, and drawing a test line graph of the material-liquid ratios and the yield;
s5: then, fixing the feed-liquid ratio at 1:17, extracting for 2h for 1 time, controlling the temperature at 60 ℃, 70 ℃, 80 ℃, 90 ℃ and 100 ℃ respectively to perform a goldenrod polysaccharide yield test, and drawing a test line graph of the temperature and the yield;
s6: finally, fixing the liquid-material ratio at 1:14, extracting at 80 ℃, extracting for 1 time, controlling the extraction time at 30min, 60min, 90min, 120min, 150min and 180min respectively to obtain the polysaccharide yield of the Solidago decurrens, and drawing test line graphs of the extraction time and the yield;
s7: through three line graphs, it can be easily found that when the optimal liquid-material ratio is 1: 14. when the temperature is 80 ℃ and 2 hours, the yield of the goldenrod herb polysaccharide is highest, the polysaccharide extraction is carried out by taking the polysaccharide as the extraction condition, the extracting solution is refluxed by 80 percent volume of absolute ethyl alcohol, then refrigerated and centrifuged by a refrigerated and centrifuged device, and the precipitate obtained by removing the supernatant is dried by a drying device, thus obtaining the crude goldenrod herb polysaccharide.
2. The method for extracting the goldenrod polysaccharide as claimed in claim 1, wherein the processing system comprises a cleaning device, a drying device, a pulverizing device, a weighing device, a filtering device, a freezing and centrifuging device and a drying device, and the pulverizing device is a Chinese herbal medicine pulverizer with model number FW-135.
3. The method for extracting goldenrod polysaccharide as claimed in claim 2, wherein the cleaning device is a numerical control ultrasonic cleaner, and the model of the numerical control ultrasonic cleaner is KQ-300 DB.
4. The method for extracting Solidago decurrens polysaccharide according to claim 3, wherein the weighing device is an electronic analytical balance, and the model of the electronic analytical balance is FA-2004B.
5. The method for extracting goldenrod polysaccharide of claim 4, wherein the freezing and centrifuging device is a bench-top high-speed freezing centrifuge, and the bench-top high-speed freezing centrifuge is H3-20 KR.
6. The method for extracting goldenrod polysaccharide of claim 5, wherein the drying device is a glass instrument airflow dryer, and the type of the glass instrument airflow dryer is C-20.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116440726A (en) * | 2022-06-16 | 2023-07-18 | 华大工程生物学长荡湖研究所 | Homogenizing circulation control method and feed liquid treatment method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105125611A (en) * | 2015-09-30 | 2015-12-09 | 嘉兴职业技术学院 | Ultrasonic extracting method for antioxidant ingredients of solidago canadensis |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105125611A (en) * | 2015-09-30 | 2015-12-09 | 嘉兴职业技术学院 | Ultrasonic extracting method for antioxidant ingredients of solidago canadensis |
Non-Patent Citations (4)
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| 何东平等主编: "《米糠加工技术》", 30 April 2014, 中国轻工业出版社 * |
| 李豫等: ""艾草与一枝黄花的红外光谱三级鉴别"", 《光谱学与光谱分析》 * |
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| 靳学远等: "《天然产物降血糖功能性成分研究》", 30 June 2009, 上海交通大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116440726A (en) * | 2022-06-16 | 2023-07-18 | 华大工程生物学长荡湖研究所 | Homogenizing circulation control method and feed liquid treatment method |
| CN116440726B (en) * | 2022-06-16 | 2024-01-19 | 华大工程生物学长荡湖研究所 | Homogenizing circulation control method and feed liquid treatment method |
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