CN112655842A - Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs - Google Patents

Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs Download PDF

Info

Publication number
CN112655842A
CN112655842A CN202011504839.0A CN202011504839A CN112655842A CN 112655842 A CN112655842 A CN 112655842A CN 202011504839 A CN202011504839 A CN 202011504839A CN 112655842 A CN112655842 A CN 112655842A
Authority
CN
China
Prior art keywords
carotenoid
crabs
shrimps
feed additive
feed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202011504839.0A
Other languages
Chinese (zh)
Inventor
曾胡龙
陶正国
程宗佳
李辉
林劲冬
崔海滨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGZHOU LEADER BIO-TECHNOLOGY CO LTD
Original Assignee
GUANGZHOU LEADER BIO-TECHNOLOGY CO LTD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GUANGZHOU LEADER BIO-TECHNOLOGY CO LTD filed Critical GUANGZHOU LEADER BIO-TECHNOLOGY CO LTD
Priority to CN202011504839.0A priority Critical patent/CN112655842A/en
Publication of CN112655842A publication Critical patent/CN112655842A/en
Pending legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Landscapes

  • Fodder In General (AREA)

Abstract

The invention discloses a preparation method of a carotenoid feed additive for improving the astaxanthin content in shrimps and crabs, which comprises the following steps: (1) screening carotenoid capable of improving astaxanthin content in bodies of shrimps and crabs, and crushing, screening by a screen or fully dissolving by a solvent to obtain standby carotenoid; (2) compounding the carotenoid to be prepared with the synergist to prepare a carotenoid compound; (3) screening and detecting the carotenoid compound to obtain the finished product of the carotenoid feed additive. The preparation method of the invention can effectively improve the application effect of the selected carotenoid in animal feed by preparing the selected carotenoid into the powdery or particulate feed additive with good fluidity, has lower preparation cost and higher application value, and is proved by animal experiment results that: the carotenoid feed additive prepared by the method can improve the astaxanthin content in the bodies of the shrimps and crabs and improve the growth performance of the shrimps and crabs.

Description

Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs
Technical Field
The invention relates to the field of preparation methods of animal feed additives, in particular to a preparation method of a carotenoid feed additive for improving the astaxanthin content in shrimps and crabs.
Background
The shrimps and crabs are important high-grade aquaculture species, are deeply loved by consumers, are widely cultured in multiple countries of the world, and have the culture yield of nearly 500 million tons, the industrial scale of several billions of yuan and huge volume in China at present. The shrimp and crab are rich in astaxanthin, which is one of the strongest antioxidants in the nature and is indispensable to the health and breeding of the shrimp and crab. The existing research reports prove that the astaxanthin is beneficial to human bodies and has important functions in the aspects of improving the immunity of the human bodies, resisting fatigue and the like. The body color of the cooked shrimps and crabs turns red, the principle is that the astaxanthin in the shrimps and crabs is heated and then separated from protein to show the original red color, and the natural red body color is also a characteristic expression of healthy shrimps and crabs and has great attraction for consumers. The shrimp and crab lacking astaxanthin are cooked to be pale, and the commodity value is obviously reduced.
The body color of the shrimps and crabs is not only an important commodity parameter, but also an important technical parameter which needs to be concerned in the shrimp and crab culture process because the health conditions of the shrimps and crabs can be reflected to a certain extent. The astaxanthin content in the bodies of the shrimps and the crabs is increased, so that the method has significance for increasing the commodity value of the shrimps and the crabs and also has important value for improving the health condition of the shrimps and the crabs. Under the condition of artificial breeding, the food source of the shrimps and crabs is mainly artificial compound feed, and the problem of poor body color often occurs in the process of breeding the shrimps and crabs, and the main reason is that the astaxanthin content in the shrimps and crabs is insufficient. In order to ensure that the artificially cultured shrimps and crabs keep normal body color, the common method at present is to directly add astaxanthin into the artificial compound feed for the shrimps and crabs. However, the astaxanthin is expensive, so that the feed cost is obviously increased by directly adding the astaxanthin feed additive into the artificial compound feed for the shrimps and crabs, and the astaxanthin feed additive becomes a pain point for limiting the development of the industry for a long time. How to improve the astaxanthin content in the bodies of the shrimps and the crabs is an important technical subject in the feed additive industry and is worthy of deep research. However, no other feed additive products which can achieve the same effect or the similar effect of the astaxanthin feed additive exist in the industry at present, and the research and development of substitute products have wide market prospect.
Carotenoids are a general term for a large group of natural products, mainly derived from plants and microorganisms, and are in a large variety, so far, more than 600 natural carotenoids such as lycopene, lutein and the like are found in nature, astaxanthin is also in the carotenoid large group, but the commodity price is far higher than that of other carotenoids. The general carotenoid feed additive product has little effect on improving the astaxanthin content in the bodies of the shrimps and crabs, has great difference with the effect of the astaxanthin feed additive product, and has little application value. Therefore, the prior art of using carotenoid feed additives for increasing the astaxanthin content in shrimp and crab bodies is not many.
Chinese patent CN104447464A provides a high-content carotenoid compound derived from Adonis amurensis, and a preparation method and a composition thereof. The method uses adonis amurensis extract as a raw material, and prepares a high-content carotenoid compound by adding alkali and alcohol for reaction, wherein the carotenoid comprises astaxanthin, semiastacin, astacin and adonis carthami, and the proportion of all-trans astaxanthin in the carotenoid is more than 80 wt%. The carotenoid produced by the method still contains astaxanthin as a main component, has high production cost, is more suitable for high-end fields such as food, health care products and the like, and is not suitable for being used as a feed additive for replacing astaxanthin and improving the astaxanthin content in the bodies of shrimps and crabs.
Chinese patent CN110916011A discloses a carotenoid feed additive and a preparation method thereof, which is used for poultry feed. The method adopts the processes of breaking the walls of the dry blakeslea trispora thallus and the dry saccharomyces cerevisiae thallus and crushing to produce the carotenoid feed additive, wherein the carotenoid comprises beta-carotene and/or lycopene. The process only carries out wall breaking and crushing treatment on dry thalli containing the carotenoid, and if the carotenoid feed additive is added into shrimp and crab feed, the effect of replacing astaxanthin and improving the astaxanthin content in the shrimp and crab bodies is difficult to achieve.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a preparation method of a carotenoid feed additive for improving the astaxanthin content in the bodies of shrimps and crabs. Under the condition of the same additive amount as the astaxanthin feed additive, the amplitude of increasing the astaxanthin content in the bodies of the shrimps and crabs is close to that of a product of the astaxanthin feed additive directly added.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of a carotenoid feed additive for improving the astaxanthin content in shrimps and crabs comprises the following steps:
(1) screening carotenoid capable of improving astaxanthin content in bodies of shrimps and crabs, and crushing, screening by a screen or fully dissolving by a solvent to obtain standby carotenoid;
(2) compounding the carotenoid to be prepared with the synergist to prepare a carotenoid compound;
(3) screening and detecting the carotenoid compound to obtain the finished product of the carotenoid feed additive.
As a preferable technical scheme, in the step (1), the carotenoid is crushed to a particle size which can pass through a sieve with 20-200 meshes.
As a preferable technical scheme, in the step (1), the solvent is one or more of ethanol, propanol, isopropanol, butanol, methyl acetate, ethyl acetate, propyl acetate, acetone, dichloromethane, n-hexane, cyclohexane and propylene oxide.
As a preferable technical scheme, in the step (2), the synergist is heated to 80-100 ℃ to be melted or dissolved in water, 1-100 parts by weight of carotenoid is added into 1-100 parts by weight of the synergist, the carotenoid and the synergist are fully and uniformly mixed after being sheared and stirred for 20-50 minutes, solid particles are formed in a spray cooling granulation mode, and the solid particles are further separated and collected through a cyclone separator to obtain the carotenoid compound.
The compound process in the step (2) is very important for the carotenoid feed additive to achieve the effect of the invention, and can play a plurality of roles. After the processing of the steps, the synergist component can effectively protect the carotenoid component, and greatly reduce the loss rate of the carotenoid component in the processing and storage processes of the shrimp and crab feed and the loss rate of the carotenoid component in the gastrointestinal tract after the shrimp and crab feed are fed. After the processing of the step, the screened carotenoid component forms micron-to-nanometer highly dispersed particles, and the absorption and utilization rate of the carotenoid component in the intestinal tracts of animals is greatly improved under the action of the synergist component. After the screened carotenoid components are absorbed by the bodies of the shrimps and the crabs, the synergist components can promote the carotenoid components to be efficiently converted into the astaxanthin in the bodies of the shrimps and the crabs, and finally the effect of improving the astaxanthin content in the bodies of the shrimps and the crabs is achieved.
As a preferred technical scheme, in the step (3), screening comprises passing the carotenoid compound through a 20-120 mesh vibrating screen.
As a preferable technical scheme, the carotenoid comprises one or more of lycopene, lutein, zeaxanthin, allozeaxanthin, canthaxanthin, alpha-/beta-/gamma-carotene, capsorubin, tunaxanthin, cryptoxanthin, neoxanthin, violaxanthin, citraxanthin, citrus xanthin, diatoxanthin, chlorenthrin, apoester and apoaldehyde.
As a preferable technical scheme, the synergist comprises one or more of stearic acid, paraffin, polyacrylic resin, sorbitan monostearate, sucrose fatty acid ester, fatty glyceride, sorbitan monooleate, stearate, phospholipid, carnauba wax, xylitol, gelatin, vegetable gum, sodium alginate, potassium alginate, ammonium alginate, agar, guar gum, gum arabic, xanthan gum, polyethylene glycol, mannitol, lignosulfonate, sucrose and white granulated sugar.
According to the invention, a specific combination of carotenoids is preferably selected, and a specific synergist is added to prepare a carotenoid compound, and the carotenoid compound is processed to finally obtain the carotenoid feed additive. The feed additive is specially designed for the shrimps and crabs, is safe and reliable, can promote the shrimps and crabs to efficiently absorb and utilize carotenoid and convert the carotenoid into astaxanthin when being added into the shrimp and crab feed, greatly improves the astaxanthin content in the shrimps and crabs, and nearly achieves the effect of directly adding the astaxanthin feed additive into the shrimp and crab feed under the condition of the same additive amount as the astaxanthin feed additive. Compared with the astaxanthin feed additive, the carotenoid feed additive has lower preparation cost and higher practical application value, is used as the feed additive for improving the astaxanthin content in the bodies of the shrimps and the crabs, and has great popularization and application values.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.
Example 1
A preparation method of a carotenoid feed additive for improving the astaxanthin content in shrimps and crabs comprises the following steps:
(1) micronizing one or more of lycopene, lutein, zeaxanthin, allozeaxanthin, canthaxanthin, alpha-/beta-/gamma-carotene, capsorubin, cryptoxanthin, neoxanthin, violaxanthin, citraxanthin, citrus xanthin, diatoxanthin, and chlorexanthin, and sieving with 120 mesh sieve to obtain carotenoid;
(2) heating one or more of stearic acid, paraffin, sorbitan monostearate, sucrose fatty acid ester, fatty glyceride, stearate, carnauba wax, xylitol, polyethylene glycol, gum arabic, and xanthan gum to 90 deg.C to melt;
50 kg of the carotenoid prepared in the step (1) is added into 100 kg of the melt prepared in the step (2) after passing through a 100-mesh vibrating screen, the melt and the melt are fully and uniformly mixed after being sheared and stirred for 30 minutes, solid particles are formed in a spray cooling granulation mode, and are further separated and collected through a cyclone separator to obtain a carotenoid compound;
(3) and (3) passing the carotenoid compound prepared in the step (2) through a 60-mesh vibrating screen, and carrying out detection, packaging and other processes to obtain 145 kg of carotenoid feed additive.
The product prepared by the preparation method of the embodiment has good stability, low preparation cost and good practical application value.
Effect example 1
In this embodiment, the adopted prawn base feed comprises, by weight: 25% of fish meal, 18% of soybean meal, 15% of peanut meal, 24% of flour, 5% of krill meal, 3% of soybean protein concentrate, 3% of squid viscera powder, 2% of fish oil, 1% of soybean phospholipid and 4% of vitamin mineral substances.
According to the prawn basal feed, the experimental feeds are divided into a blank feed, a control feed and a treatment feed, wherein the blank feed is the prawn basal feed without any pigment, the control feed adopts astaxanthin feed additives to replace part of flour on the basis of the prawn feed, the treatment feed adopts carotenoid feed additives to replace part of flour on the basis of the prawn feed, and the specific formula of the three feeds is detailed in the following table 1.
TABLE 1
Figure BDA0002844652800000071
Figure BDA0002844652800000081
The experimental process comprises the following steps: and carrying out litopenaeus vannamei breeding comparison experiments on the three groups of prawn feeds. The experiment is provided with 1 blank group, 1 control group and 1 treatment group, wherein the prawns in the blank group are fed with blank group feed, the prawns in the control group are fed with control group feed, and the prawns in the treatment group are fed with treatment group feed, and each group is provided with 4 replicates. A12-mouth glass fiber reinforced plastic drum with the volume of 500L and the other conditions consistent is selected for carrying out the experiment. The method is characterized in that natural seawater is used as a water source, the salinity of water in each barrel is controlled to be 28-32 per thousand, the pH is controlled to be 7.8-8.5, the dissolved oxygen is controlled to be more than 6.0ppm, the ammonia nitrogen is controlled to be less than 0.6ppm, nitrite is controlled to be less than 0.3ppm, the water temperature is 28-32 ℃, and the water quality is mainly adjusted and controlled through sewage discharge and water change. 40 tails of the second-generation litopenaeus vannamei of healthy litopenaeus vannamei with consistent specifications are stocked in each experimental barrel, the litopenaeus vannamei is cultured for 8 weeks, and 3 meals of feed are fed every day.
The experimental results are as follows: in the above experiment, the actual cultivation time of each group of prawns is 56 days, and the growth performance index of each group of prawns is detected after the experiment is finished, and the results are shown in the following table 2, which shows that the carotenoid feed additive prepared by the preparation method can obviously and effectively improve the astaxanthin content in the prawns, and the effect of improving the astaxanthin content in the prawns can approach the effect of directly adding the astaxanthin feed additive.
TABLE 2
Figure BDA0002844652800000082
Effect example 2
In the embodiment, the adopted crab basic feed comprises the following components in percentage by weight: 20% of fish meal, 25% of soybean meal, 12% of peanut meal, 5% of rapeseed meal, 18% of flour, 5% of krill meal, 3% of soybean protein concentrate, 3% of squid viscera powder, 2% of fish oil, 1% of yeast powder, 1% of soybean phospholipid and 5% of vitamin mineral substances.
According to the crab basic feed, the experimental feeds are divided into a blank feed, a control feed and a treatment feed, wherein the blank feed is the crab basic feed without any pigment, the control feed adopts an astaxanthin feed additive to replace part of flour on the basis of the crab feed, the treatment feed adopts a carotenoid feed additive to replace part of flour on the basis of the crab feed, and the specific formula of the three feeds is detailed in the following table 3.
TABLE 3
Figure BDA0002844652800000091
Figure BDA0002844652800000101
The experimental process comprises the following steps: and carrying out a eriocheir sinensis breeding comparison experiment on the three groups of crab feeds. The experiment is provided with 1 blank group, 1 control group and 1 treatment group, the Chinese mitten crabs in the blank group are fed with the blank group of feed, the Chinese mitten crabs in the control group of feed and the Chinese mitten crabs in the treatment group of feed, and each group is provided with 4 replicates. A12-opening glass fiber reinforced plastic square barrel with the specification of 50cm multiplied by 60cm multiplied by 100cm and other conditions are consistent is selected to carry out experiments. The aerated tap water is used as a water source, the water temperature of water in each barrel is controlled to be 25-28 ℃, the pH value is controlled to be 7.2-7.8, the dissolved oxygen is controlled to be more than 6.0ppm, the ammonia nitrogen is controlled to be less than 0.3ppm, the nitrite is controlled to be less than 0.1ppm, and the water quality is controlled by a circulating water system and proper water change. 15 young crabs of healthy eriocheir sinensis crabs with consistent specifications are put in each experimental barrel, and are cultured for 8 weeks, and 2 meals of feed are fed in the morning and at the evening each day.
The experimental results are as follows: in the above experiment, the actual cultivation time of each group of eriocheir sinensis is 60 days, and the growth performance index of the eriocheir sinensis is detected after the experiment is finished, and the results are shown in the following table 4, which shows that the carotenoid feed additive prepared by the preparation method can obviously and effectively improve the astaxanthin content in the eriocheir sinensis, and the effect of improving the astaxanthin content in prawns can be close to the effect of directly adding the astaxanthin feed additive.
TABLE 4
Figure BDA0002844652800000102
Figure BDA0002844652800000111
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.

Claims (7)

1. A preparation method of a carotenoid feed additive for improving the astaxanthin content in shrimps and crabs is characterized by comprising the following steps:
(1) screening carotenoid capable of improving astaxanthin content in bodies of shrimps and crabs, and crushing, screening by a screen or fully dissolving by a solvent to obtain standby carotenoid;
(2) compounding the carotenoid to be prepared with the synergist to prepare a carotenoid compound;
(3) screening and detecting the carotenoid compound to obtain the finished product of the carotenoid feed additive.
2. The method according to claim 1, wherein in the step (1), the carotenoid is pulverized so that the particle size can pass through a 20-200 mesh sieve.
3. The method according to claim 1, wherein in the step (1), the solvent is one or more selected from ethanol, propanol, isopropanol, butanol, methyl acetate, ethyl acetate, propyl acetate, acetone, dichloromethane, n-hexane, cyclohexane and propylene oxide.
4. The preparation method according to claim 1, wherein in the step (2), the synergist is heated to 80-100 ℃ to be melted or dissolved in water, 1-100 parts by weight of the carotenoid is added into 1-100 parts by weight of the synergist, the carotenoid and the synergist are mixed uniformly by shearing and stirring for 20-50 minutes, solid particles are formed by means of spray cooling granulation, and the solid particles are separated and collected by a cyclone separator to obtain the carotenoid compound.
5. The method according to claim 1, wherein the screening in step (3) comprises passing the carotenoid complex through a 20-120 mesh sieve shaker.
6. The method of claim 1, wherein the carotenoid comprises one or more of lycopene, lutein, zeaxanthin, allozeaxanthin, canthaxanthin, α -/β -/γ -carotene, capsorubin, tunaxanthin, cryptoxanthin, neoxanthin, violaxanthin, citraxanthin, citrus xanthin, diatoxanthin, chlorexanthin, apoester, and apoaldehyde.
7. The method of claim 1, wherein the synergist comprises one or more of stearic acid, paraffin, polyacrylic resin, sorbitan monostearate, sucrose fatty acid ester, fatty glyceride, sorbitan monooleate, stearate, phospholipid, carnauba wax, xylitol, gelatin, vegetable gum, sodium alginate, potassium alginate, ammonium alginate, agar, guar gum, gum arabic, xanthan gum, polyethylene glycol, mannitol, lignosulfonate, sucrose, and white granulated sugar.
CN202011504839.0A 2020-12-18 2020-12-18 Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs Pending CN112655842A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011504839.0A CN112655842A (en) 2020-12-18 2020-12-18 Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011504839.0A CN112655842A (en) 2020-12-18 2020-12-18 Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs

Publications (1)

Publication Number Publication Date
CN112655842A true CN112655842A (en) 2021-04-16

Family

ID=75406471

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011504839.0A Pending CN112655842A (en) 2020-12-18 2020-12-18 Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs

Country Status (1)

Country Link
CN (1) CN112655842A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6419398A (en) * 1997-08-13 1999-03-08 Kagome Co., Ltd. Water-dispersible carotenoid pigment preparation
CN101396068A (en) * 2007-09-27 2009-04-01 浙江医药股份有限公司新昌制药厂 Carotenoid preparation and use thereof and feedstuff containing the preparation and preparation method thereof
CN101530172A (en) * 2009-04-23 2009-09-16 中国水产科学研究院珠江水产研究所 Synergistic agent for promoting coloring effect of carotenoid colorant on finishes
CN104447464A (en) * 2013-09-24 2015-03-25 浙江医药股份有限公司新昌制药厂 Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6419398A (en) * 1997-08-13 1999-03-08 Kagome Co., Ltd. Water-dispersible carotenoid pigment preparation
CN101396068A (en) * 2007-09-27 2009-04-01 浙江医药股份有限公司新昌制药厂 Carotenoid preparation and use thereof and feedstuff containing the preparation and preparation method thereof
CN101530172A (en) * 2009-04-23 2009-09-16 中国水产科学研究院珠江水产研究所 Synergistic agent for promoting coloring effect of carotenoid colorant on finishes
CN104447464A (en) * 2013-09-24 2015-03-25 浙江医药股份有限公司新昌制药厂 Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
徐在宽: "《虾蟹饲料与病害防治专家谈》", 30 November 2001, 科学技术文献出版社 *

Similar Documents

Publication Publication Date Title
AU605082B2 (en) Haematococcus alga pigmentation supplement composition
CN101978854A (en) Granular feed for sea cucumbers and preparation method
CN103865798A (en) Method for wall breaking of phaffia rhodozyma through enzymolysis and application thereof
CN106616000B (en) Two river crab color control feeds and feeding method thereof
CN1864522A (en) A method for preparing environment friendly expanded mixed feedstuff for trachinotusovatus
CN101756021B (en) Compound feed for culturing sea cucumbers
CN105076745A (en) Macrobrachium rosenbergii disease preventing Chinese herbal medicine feed and processing method thereof
CN103734502A (en) Tropical fish feed, and preparation method thereof
CN104431503A (en) Functional type soft-shelled turtle compound feed
CN111772063A (en) Compound feed for improving blue body of penaeus vannamei boone under industrial cultivation and preparation method thereof
CN103652495A (en) Megalobatrachusdavidianus pellet feed and preparation method thereof
CN104171646A (en) Freshwater shrimp feed
CN104905030B (en) Ussuriensis larva and juvenile special compound feed and preparation method thereof
CN114027420A (en) Selenium-rich organic litopenaeus vannamei and macrobrachium rosenbergii polyculture feed and preparation method thereof
CN113519726A (en) Color-mixing compound feed for improving body color and quality of large yellow croaker
CN104447464A (en) Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound
CN111567700A (en) Alga composition for aquaculture, aquaculture feed and aquaculture method
CN101386879A (en) Method for preparing astaxanthin ester
CN115968985A (en) Low-temperature stress resistant feed for freshwater lobsters as well as preparation method and application of low-temperature stress resistant feed
CN112655842A (en) Preparation method of carotenoid feed additive for improving astaxanthin content in shrimps and crabs
CN112655840A (en) Special feed for industrial culture of penaeus vannamei boone and preparation method thereof
CN102217733A (en) Flaky hatch feed for prawn seedlings
CN102578405B (en) Pseudobagrus ussuriensis special compound feed and preparation method thereof
CN110235989B (en) Marine microalgae meal composite nutrient microcapsule and preparation method and application thereof
CN116138368A (en) Shellfish aquatic animal feed additive and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20210416

RJ01 Rejection of invention patent application after publication