CN112649493A - Device and method for simultaneously detecting ammonia gas and nitric oxide in exhaled breath - Google Patents
Device and method for simultaneously detecting ammonia gas and nitric oxide in exhaled breath Download PDFInfo
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- CN112649493A CN112649493A CN202011484962.0A CN202011484962A CN112649493A CN 112649493 A CN112649493 A CN 112649493A CN 202011484962 A CN202011484962 A CN 202011484962A CN 112649493 A CN112649493 A CN 112649493A
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- tube
- sample inlet
- ion mode
- exhaled breath
- nitric oxide
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/622—Ion mobility spectrometry
Abstract
The invention discloses a device and a method for simultaneously detecting ammonia and nitric oxide in exhaled breath, and belongs to the field of analytical chemical instruments. The device consists of a positive ion mode migration tube, a negative ion mode migration tube, an ionization source and an exhalation pipeline; the positive ion mode migration tube is respectively provided with a floating gas inlet, a sample inlet and a gas outlet; the negative ion mode migration tube is respectively provided with a floating gas inlet, a sample inlet and a gas outlet; the expired air pipeline is provided with a sample inlet and two air outlets, one air outlet is directly connected with the sample inlet of the positive ion mode migration pipe, the other air outlet is connected with the dehumidification device and then connected with the sample inlet of the negative ion mode migration pipe, and the two migration pipes are respectively connected with the ionization source close to the sample inlet end. The method can simultaneously detect ammonia gas and nitric oxide in the exhaled breath, and provides favorable help for screening and diagnosing diseases.
Description
Technical Field
The invention belongs to the field of analytical chemical instruments, and particularly relates to a device and a method for simultaneously detecting ammonia and nitric oxide in exhaled breath.
Background
The human metabolic products are closely related to the components in exhaled breath, and many substances in exhaled breath can be used as markers for disease diagnosis. For example, ammonia gas can be used as a marker for hepatic encephalopathy, renal failure, etc., and nitric oxide can be used as a marker for asthma diagnosis. Compared with other detection methods, such as blood detection, the exhaled breath detection has the advantages of no wound, convenient sampling and the like, and has wide application prospect in early disease screening. The ion mobility spectrometry instrument has the advantages of small volume, low energy consumption, capability of working under atmospheric pressure, high detection speed and the like, and is gradually applied to the field of exhaled breath detection in recent years. However, at present, the nitrogen oxide and the ammonia gas in the exhaled breath can only be detected by using a negative ion mode migration tube and a positive ion mode migration tube respectively, although the nitrogen oxide can also be detected by using a positive and negative switching migration tube, the influence of the humidity on the nitrogen oxide is very large, the humidity in the exhaled breath is close to 100% RH, a water removal device is required to be used for separating the water in advance, and the ammonia gas can be removed by using a water removal device such as a nafion tube and a semiconductor during water removal, so that the requirement for simultaneously detecting the ammonia gas and the nitrogen oxide in the exhaled breath cannot be met by using a positive and negative switching ion mobility spectrometer.
The invention discloses a high-voltage power supply and a method for realizing simultaneous detection of positive and negative ions by a single-tube ion mobility spectrometer, and the high-voltage power supply comprises a control area high voltage part and a drift area high voltage part, and further comprises a high-voltage positive and negative polarity switching control mechanism, wherein the high-voltage positive and negative polarity switching control mechanism successively applies positive and negative high voltages to a drift tube in a single analysis process of the ion mobility spectrometer, and controls the time sequence of an ion trap and a gate under the positive and negative high voltages, so that simultaneous detection of positive and negative ions in the single analysis process is effectively finished, the control area high voltage and the drift area high voltage are converted by two different DC-DC modules, the same direct voltage is input, different high voltages are output, and the output high voltages are respectively applied to a control area. The single-tube ion mobility spectrometry can realize more than 40 times of positive and negative mode switching within 10 seconds of one-time analysis, alternately detect positive and negative ions and simultaneously give out the detection results of the positive and negative ions. But cannot detect exhaled breath at high humidity.
Disclosure of Invention
Aiming at the problem that the prior art cannot simultaneously detect ammonia gas and nitric oxide in high-humidity exhaled breath, the invention provides a device and a method for simultaneously detecting ammonia gas and nitric oxide in high-humidity exhaled breath based on an ion mobility spectrometry instrument.
The invention provides a device for simultaneously detecting ammonia and nitric oxide in exhaled breath, which comprises a negative ion mode migration tube, wherein the negative ion mode migration tube is connected with a positive ion mode migration tube through an ionization source, a sample inlet of the positive ion mode migration tube is connected with one end of a sample inlet tube a, the other end of the sample inlet tube a is connected with one end of a flow dividing tube, the other end of the flow dividing tube is connected with one end of a sample inlet tube b, the other end of the sample inlet tube b is connected with a sample inlet of the negative ion mode migration tube, the side surface of the flow dividing tube is communicated with one end of a sample inlet tube c, and the other end of the sample inlet tube c is a sample inlet; a floating gas inlet b and a gas outlet b are arranged on the negative ion mode migration pipe; a floating gas inlet a and a gas outlet a are arranged on the positive ion mode migration pipe; the sample inlet pipe b is provided with a dehumidifying device.
Further, in the above technical scheme, the humidity of the exhaled breath is 99% RH-100% RH.
Further, in the above technical solution, the ionization source includes but is not limited to a nickel source, a corona discharge ionization source, or a vacuum ultraviolet lamp ionization source.
Further, in the above technical scheme, the gas entering the floating gas inlet is filtered clean air or nitrogen.
Further, in the above technical solution, the dehumidifying device includes, but is not limited to, a nafion tube.
Further, in the above technical solution, the dehumidifying device is a semiconductor refrigeration device.
Further, in the above technical scheme, the sample inlet is connected with the exhaled air.
Furthermore, in the above technical solution, the negative ion mode migration tube, the ionization source and the negative ion mode migration tube are on the same horizontal plane; the sampling pipe b and the sampling pipe a are arranged in parallel.
Furthermore, in the above technical solution, the connection point of the sample injection tube b and the negative ion mode migration tube is close to the position of the ionization source; the connection point of the sampling tube a and the positive ion mode migration tube is close to the position of the ionization source.
Further, in the above technical scheme, the floating gas inlet b, the gas outlet b, the floating gas inlet a and the gas outlet a are all far away from the position of the ionization source.
The invention also provides a detection method using the device, exhaled air reaches the shunt tube through the sample inlet tube c from the sample inlet, the shunt tube divides the exhaled air into the sample inlet tube a and the sample inlet tube b, and the exhaled air of the sample inlet tube a enters the positive ion mode migration tube to detect ammonia gas; and the expired air of the sampling tube b enters the anion mode migration tube through the dehumidifying device to detect the nitric oxide.
Has the advantages that: the invention provides a device and a method for simultaneously detecting ammonia gas and nitric oxide in exhaled breath based on an ion mobility spectrometry instrument.
Drawings
Fig. 1 is a schematic structural diagram of the device for simultaneously detecting ammonia and nitric oxide in exhaled breath according to the present invention.
In the figure, 1, a floating gas inlet b; 2. a negative ion mode transfer tube; 3. an ionization source; 4. a positive ion mode mobility tube; 5. a floating gas inlet a; 6. an air outlet a; 7. a sample inlet; 8. a sample inlet pipe a; 9. a sample inlet pipe b; 10. a dehumidifying device; 11. an air outlet b; 12. a sample inlet pipe c; 13. and (4) dividing the tube.
Detailed Description
The following non-limiting examples will allow one of ordinary skill in the art to more fully understand the present invention, but are not intended to limit the invention in any way.
Example 1
As shown in fig. 1, a device for simultaneously detecting ammonia and nitric oxide in exhaled breath comprises a negative ion mode migration tube 2, wherein the negative ion mode migration tube 2 is connected with a positive ion mode migration tube 4 through an ionization source 3, a sample inlet of the positive ion mode migration tube 4 is connected with one end of a sample inlet tube a8, the other end of the sample inlet tube a8 is connected with one end of a shunt tube 13, the other end of the shunt tube 13 is connected with one end of a sample inlet tube b9, the other end of the sample inlet tube b9 is connected with a sample inlet of the negative ion mode migration tube 2, the side surface of the shunt tube 13 is communicated with one end of a sample inlet tube c 12, and the other end of the sample inlet tube c 12 is a sample inlet 7; a floating gas inlet b1 and a gas outlet b11 are arranged on the anion mode migration pipe 2; a floating gas inlet a5 and a gas outlet a6 are arranged on the positive ion mode migration pipe 4; the sample injection pipe b9 is provided with a dehumidifier 10.
Example 2
In a detection method using the device in example 1, the exhaled air reaches the shunt tube 13 from the sample inlet 7 through the sample inlet tube c 12, the shunt tube 13 divides the exhaled air into the sample inlet tube a8 and the sample inlet tube b9, and the exhaled air of the sample inlet tube a8 enters the positive ion mode migration tube 4 for ammonia gas detection; the expired air from the sampling tube b9 enters the anion mode migration tube 2 through the dehumidifying device 10 to detect the nitric oxide.
Claims (10)
1. The device for simultaneously detecting ammonia gas and nitric oxide in exhaled breath is characterized by comprising a negative ion mode migration tube (2), wherein the negative ion mode migration tube (2) is connected with a positive ion mode migration tube (4) through an ionization source (3), a sample inlet of the positive ion mode migration tube (4) is connected with one end of a sample inlet tube a (8), the other end of the sample inlet tube a (8) is connected with one end of a shunt tube (13), the other end of the shunt tube (13) is connected with one end of a sample inlet tube b (9), the other end of the sample inlet tube b (9) is connected with a sample inlet of the negative ion mode migration tube (2), the side surface of the shunt tube (13) is communicated with one end of a sample inlet tube c (12), and the other end of the sample inlet tube c (12) is a sample inlet (7); a floating gas inlet b (1) and a gas outlet b (11) are arranged on the anion mode migration pipe (2); a floating gas inlet a (5) and a gas outlet a (6) are arranged on the positive ion mode migration pipe (4); the sample inlet pipe b (9) is provided with a dehumidifying device (10).
2. The apparatus of claim 1, wherein the apparatus is configured to simultaneously detect ammonia and nitric oxide in exhaled breath: the ionization source (3) includes, but is not limited to, a nickel source, a corona discharge ionization source, or a vacuum ultraviolet lamp ionization source.
3. The apparatus of claim 1, wherein the apparatus is configured to simultaneously detect ammonia and nitric oxide in exhaled breath: the air entering the air floating inlet is filtered clean air or nitrogen.
4. The method of claim 1, wherein the method comprises the steps of: the dehumidification device (10) includes, but is not limited to, a nafion tube.
5. The method of claim 1, wherein the method comprises the steps of: the dehumidifier (10) is a semiconductor refrigeration device.
6. The method of claim 1, wherein the method comprises the steps of: the sample inlet (7) is connected with the exhaled air.
7. The device for the simultaneous detection of ammonia and nitric oxide in exhaled breath according to claim 1, characterized in that the anion mode drift tube (2), the ionization source (3) and the anion mode drift tube (4) are on the same horizontal plane; the sampling tube b (9) and the sampling tube a (8) are arranged in parallel.
8. The apparatus for simultaneously detecting ammonia and nitric oxide in exhaled breath according to claim 1, wherein the connection point of the sample inlet tube b (9) and the anion mode drift tube (2) is close to the ionization source (3); the connection point of the sampling tube a (8) and the positive ion mode migration tube (4) is close to the position of the ionization source (3).
9. The device for simultaneously detecting ammonia and nitric oxide in exhaled breath according to claim 1, wherein the drift gas inlet b (1), the gas outlet b (11), the drift gas inlet a (5) and the gas outlet a (6) are all far away from the ionization source (3).
10. A method of testing using the device of any of claims 1-9, wherein the exhaled breath is passed from the sample inlet (7) through the sample inlet c (12) to the shunt tube (13), the shunt tube (13) splits the exhaled breath into the sample inlet a (8) and the sample inlet b (9), and the exhaled breath from the sample inlet a (8) is passed into the positive ion mode migration tube (4) for ammonia gas testing; the expired air of the sampling tube b (9) enters the anion mode migration tube (2) through the dehumidifying device (10) to detect nitric oxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011484962.0A CN112649493A (en) | 2020-12-15 | 2020-12-15 | Device and method for simultaneously detecting ammonia gas and nitric oxide in exhaled breath |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011484962.0A CN112649493A (en) | 2020-12-15 | 2020-12-15 | Device and method for simultaneously detecting ammonia gas and nitric oxide in exhaled breath |
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| CN112649493A true CN112649493A (en) | 2021-04-13 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010019844A1 (en) * | 1999-01-14 | 2001-09-06 | Extraction Systems, Inc. | Detection of base contaminants in gas samples |
| CN103868974A (en) * | 2012-12-12 | 2014-06-18 | 中国科学院大连化学物理研究所 | Method for detecting No and/or propofol in expiratory gas |
| CN104715999A (en) * | 2013-12-15 | 2015-06-17 | 中国科学院大连化学物理研究所 | Ionic mobility spectrometer and air path control method thereof |
| CN104707448A (en) * | 2013-12-15 | 2015-06-17 | 中国科学院大连化学物理研究所 | Gas dewatering device and using method thereof |
| CN105092689A (en) * | 2014-05-20 | 2015-11-25 | 中国科学院大连化学物理研究所 | Real time on-line expired air monitor |
-
2020
- 2020-12-15 CN CN202011484962.0A patent/CN112649493A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010019844A1 (en) * | 1999-01-14 | 2001-09-06 | Extraction Systems, Inc. | Detection of base contaminants in gas samples |
| CN103868974A (en) * | 2012-12-12 | 2014-06-18 | 中国科学院大连化学物理研究所 | Method for detecting No and/or propofol in expiratory gas |
| CN104715999A (en) * | 2013-12-15 | 2015-06-17 | 中国科学院大连化学物理研究所 | Ionic mobility spectrometer and air path control method thereof |
| CN104707448A (en) * | 2013-12-15 | 2015-06-17 | 中国科学院大连化学物理研究所 | Gas dewatering device and using method thereof |
| CN105092689A (en) * | 2014-05-20 | 2015-11-25 | 中国科学院大连化学物理研究所 | Real time on-line expired air monitor |
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