CN112646700A

CN112646700A - Sequencing instrument

Info

Publication number: CN112646700A
Application number: CN201910964855.9A
Authority: CN
Inventors: 黄立平; 孙磊林; 陈泽华; 余利; 陈文�; 李骏杨; 刘卓; 李开金
Original assignee: MGI Tech Co Ltd
Current assignee: MGI Tech Co Ltd
Priority date: 2019-10-11
Filing date: 2019-10-11
Publication date: 2021-04-13
Anticipated expiration: 2039-10-11
Also published as: CN112646700B

Abstract

A sequencer, the sequencer comprising: the biochemical platform component is used for generating gene sequencing reaction on a sample in the sequencing chip; the optical detection component is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing reaction; the manipulator assembly is used for transferring the sequencing chip between the optical detection assembly and the biochemical platform assembly; after the biochemical platform assembly carries out biochemical reaction, the mechanical arm assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection. The sequencer is high in flexibility, high in sequencing speed and low in sequencing cost.

Description

Sequencing instrument

Technical Field

The invention relates to a sequencer.

Background

The gene sequencing technology is widely applied to whole genome sequencing, transcriptome sequencing, metagenome sequencing and the like at present, is a powerful tool for analyzing biochemical evolution and classification, researching disease-related genes such as cancer, autism and the like, performing in-vitro diagnosis and the like, promotes people to further understand life science, and also promotes the development of health industry.

In the prior art, a gene sequencer is often used to perform gene sequencing on a sample in a flow cell. The flow cell is also called a sequencing chip, and the sequencing chip is an area for loading a gene sequencing sample and carrying out a sequencing reaction. Due to the non-repeatability of sample loading and the requirement of avoiding cross contamination between different samples, sequencing chips are usually designed to be disposable, repeatedly assembled and disassembled, and in a totally enclosed manner. The lower surface of the sequencing chip is usually a substrate, and the gene sample to be tested can be fixed on the surface of the sequencing chip through some biological or chemical reaction. Because the sequencing chip is transferred to the signal detection system for signal detection after the sequencing reaction, the sequencing reaction can be carried out again after the signal detection, or the transfer of the waste chip after the sequencing is finished manually, and the gene sequencer commonly used in the prior art can only run one or two sequencing chips simultaneously, thereby the gene sequencer in the prior art has the problems of poor flexibility, low sequencing speed, high cost and the like.

Disclosure of Invention

Accordingly, the present invention provides a sequencer capable of solving the above-mentioned problems.

A sequencer, the sequencer comprising: the biochemical platform component is used for generating gene sequencing reaction on a sample in the sequencing chip; the optical detection component is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing; the manipulator assembly is used for transferring the sequencing chip between the optical detection assembly and the biochemical platform assembly; after the biochemical platform assembly carries out biochemical reaction, the mechanical arm assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection.

Furthermore, the sequencer also comprises a refrigerating device, wherein the refrigerating device is connected with the biochemical platform assembly and is used for providing sequencing reagents required by gene sequencing reaction for the biochemical platform assembly.

Furthermore, the refrigerating device comprises a refrigerating cavity and a refrigerating module fixed at one end of the refrigerating cavity; the cold storage cavity is used for accommodating and refrigerating sequencing reagents, the sequencing reagents are arranged in a sequencing reagent box, and the sequencing reagent box is placed in the cold storage cavity; the refrigeration module is used for making cold air flow in the refrigeration cavity body so as to refrigerate the sequencing reagent.

Furthermore, the sequencer also comprises a cleaning reagent kit component, and the cleaning reagent kit component is used for cleaning the pipeline, the sequencing chip and the biochemical platform component.

Furthermore, the cleaning kit component comprises a cleaning kit and a drawer, the cleaning kit is accommodated in the drawer, one end of the cleaning kit is provided with a boss, a side wall of the drawer is provided with a notch, and the boss is accommodated in the notch.

Furthermore, the sequencer also comprises a photoelectric sensor, the photoelectric sensor is opposite to one end of the cleaning reagent box assembly, and the photoelectric sensor is used for detecting whether the cleaning reagent box is put in and whether the drawer is pushed to the right position; when the washing reagent box is placed in the drawer and the drawer is pushed to a specified position, the photoelectric sensor is triggered.

Furthermore, the sequencer also comprises a shell and a double-shaft rotating structure connected to the shell, wherein the double-shaft rotating structure comprises a movable door and two double-shaft rotating shaft blocks, and each double-shaft rotating shaft block comprises a first rotating shaft and a second rotating shaft; the shell comprises two frames which are oppositely arranged; two adjacent surfaces of the two frames are respectively provided with a first track hole and a second track hole, the first track hole is positioned above the second track hole, the first track hole and the second track hole are both arc-shaped, and the bending directions of the first track hole and the second track hole are opposite; the double-shaft rotating shaft block is fixed at one end of the movable door, the first rotating shaft is accommodated in the first track hole, and the second rotating shaft is accommodated in the second track hole; pulling one end of the movable door, which is far away from the double-shaft rotating shaft block, and enabling the first rotating shaft and the second rotating shaft to slide in the first track hole and the second track hole so as to open the movable door; and pushing the movable door in the opposite direction, wherein the first rotating shaft and the second rotating shaft slide in the first track hole and the second track hole in the opposite direction to close the movable door.

Furthermore, a special-shaped groove is formed on the shell, the sequencer further comprises a light guide assembly, the light guide assembly comprises a light guide plate, a light guide post and a lamp strip, the light guide plate, the light guide post and the lamp strip are contained in the special-shaped groove, the light guide plate faces the outside, the light guide post is fixed on the light guide plate, and the lamp strip is attached to the light guide post; the light guide plate is provided with an air inlet, the light guide column and the lamp strip are identical in shape, and the light guide column, the air inlet and the lamp strip are identical in shape.

Furthermore, the shell is also provided with a chip inlet, and the chip inlet is used for placing a sequencing chip from the outside; the sequencer also comprises a chip driver, the chip driver is opposite to the chip inlet and is used for receiving the sequencing chip from the chip inlet, and the chip driver can be ejected out of the chip inlet; and the manipulator component grabs the sequencing chip from the core drive and transfers the sequencing chip to the biochemical platform component for biochemical reaction.

Furthermore, the sequencer also comprises a waste chip collecting component, and the waste chip collecting component is used for collecting waste chips after sequencing; the manipulator assembly transfers the sequencing chip into the waste chip collecting assembly after the sequencing is finished; the waste chip collecting assembly comprises a collecting bin door and a waste chip collecting bin, wherein the collecting bin door is connected to the waste chip collecting bin and pushes the lower part of the collecting bin door, and the collecting bin door is opened to take out the waste chips.

The sequencer provided by the invention can 1) simultaneously carry out gene sequencing work of a plurality of sequencing chips without mutual interference, thereby increasing the sequencing speed and reducing the cost; 2) the manipulator assembly is arranged, so that the automation degree of the sequencer can be improved, the sequencing speed and the sequencing flux can be further improved, and the cost can be reduced; 3) a photoelectric sensor is arranged at one end of the cleaning kit component, so that whether the cleaning kit is put in and whether the drawer is pushed in place can be detected; 4) the cold storage device comprises a cold storage cavity and a refrigeration module fixed at one end of the cold storage cavity, and can produce cold air and make the cold air flow in the cold storage cavity, so that the test reagent is refrigerated; 5) a movable door is connected to the shell by adopting a double-shaft rotating structure, so that the gap between the movable door and the shell caused by matching parts (such as a connecting piece and the like) can be reduced, and the attractiveness of the shell is enhanced; 6) the light guide assembly is combined with the air inlet hole, so that the lighting effect can be enhanced, and the heat can be dissipated.

Drawings

FIG. 1 is a schematic perspective view of a sequencer according to the present invention.

FIG. 2 is a schematic perspective view of the sequencer shown in FIG. 1 in another orientation.

FIG. 3 is a schematic diagram of a maintenance door in the sequencer shown in FIG. 1.

FIG. 4 is a schematic view of the structure of the biaxial rotation structure and a partially enlarged view of the biaxial rotation structure in the sequencer shown in FIG. 1.

FIG. 5 is a schematic diagram of a light guide assembly in the sequencer shown in FIG. 1.

FIG. 6 is a schematic perspective view of the sequencer shown in FIG. 1 with the housing removed.

FIG. 7 is another inverted perspective view of the sequencer shown in FIG. 1 with the housing removed, as shown in FIG. 6.

FIG. 8 is a schematic perspective view of a refrigerator in the sequencer shown in FIG. 6.

Fig. 9 is a sectional view taken along a sectional line IX-IX of the refrigerator shown in fig. 8.

Fig. 10 is a schematic perspective view of a refrigeration module in the refrigeration apparatus shown in fig. 9.

FIG. 11 is a schematic perspective view of a wash kit assembly of the sequencer shown in FIG. 6.

FIG. 12 is a schematic perspective view of a waste chip collection assembly in the sequencer shown in FIG. 6.

Description of the main elements

Sequencer 100

Casing 110

Front base case 111

First panel portion 1111

Second panel part 1112

Third panel part 1113

Display screen 1114

Movable door 1115

Single door 1116

Chip inlet 1117

Core drive button 1118

Rear base shell 112

Air outlet 1121

First side base shell 113

First opening 1131

First air inlet 1132

Second side base shell 114

Maintenance door 1141

Second air inlet 1142

Electronic lock 1143

Lock 1144

Lock hole 1145

Bottom base shell 115

Securing leg 1151

Frame 20

First trace hole 21

Second trace hole 22

Double-shaft rotating shaft block 10

First rotating shaft 11

Second rotating shaft 12

Light guide assembly 30

Light guide plate 31

Air inlet 311

Light guide column 32

Lamp belt 33

Special-shaped groove 34

Frame assembly 120

Refrigeration device 130

Refrigeration cavity 40

Housing layer 41

Insulating layer 42

Cold storage inner container layer 43

Cold air circulating layer 44

Inner layer 45

Refrigeration module 50

TEC component 51

Cold air fin 52

Cold air fan 53

Heat sink 54

Heat radiation fan 55

Biochemical platform assembly 140

Reagent needle 141

Optical detection assembly 150

Robot assembly 160

Core drive 170

Cleaning kit assembly 180

Cleaning kit 61

Boss 611

Drawer 62

Notch 621

Photoelectric sensor 70

Waste chip collecting assembly 190

Collection bin gate 191

Waste chip collecting bin 192

Shield cover 194

Second opening 195

Air inlet channel 101

Heat dissipation channel 102

The following detailed description will further illustrate the invention in conjunction with the above-described figures.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 12 in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Referring to FIG. 1, a sequencer 100 is provided in accordance with a preferred embodiment of the present invention, the sequencer 100 being used to analyze biochemical substances (gene sequences). The sequencer 100 includes a housing 110, a frame assembly 120, four refrigerators 130, four biochemical platform assemblies 140, an optical inspection assembly 150, a manipulator assembly 160, a core driver 170, four cleaning reagent cartridge assemblies 180, and a waste chip collection assembly 190. The housing 110 is fixed to and wrapped around the frame assembly 120, and the refrigerator 130, the biochemical platform assembly 140, the optical detection assembly 150, the robot assembly 160, the core driver 170, the cleaning reagent cartridge assembly 180, and the waste chip collecting assembly 190 are accommodated in the frame assembly 120.

In other embodiments, the number of the refrigeration device 130, the biochemical platform assembly 140 and the washing reagent kit assembly 180 is not limited to 4, but may be 1, 2, 3 or more than 4.

In the present embodiment, the housing 110 includes a front base case 111, a rear base case 112 facing away from the front base case 111, a first side base case 113 connected to the front base case 111 and the rear base case 112, a second side base case 114 opposite to the first side base case 113, and a bottom base case 115 connecting the front base case 111, the rear base case 112, the first side base case 113, and the second side base case 114. Of course, in other embodiments, the housing 110 further includes a top base housing (not numbered) opposite the bottom base housing 115.

In this embodiment, the front base 111 includes a first panel 1111, a second panel 1112, and a third panel 1113. The second panel portion 1112 is located between the first panel portion 1111 and the third panel portion 1113.

In this embodiment, the first panel 1111, the second panel 1112 and the upper half of the third panel 1113 may be designed as a single door or a double door, and locked by an electronic lock (not shown), and unlocked and opened only when the sequencer 100 has a problem in operation, thereby facilitating maintenance. The lower half of the first panel portion 1111 is a single door 1116, and one end of the single door 1116 is fixed to the frame assembly 120. The single-door 1116 has one end connected to the frame assembly 120 by a hinge or a hinge, and the other end fixed to the frame assembly 120 by a screw. When the single-door 1116 needs to be opened, the single-door 1116 can be opened by rotating it only by removing or unscrewing the screw with a tool. The screw fixation is used for safety reasons to avoid the user opening the single-door 1116 at will.

In this embodiment, a double door can be formed between the lower halves of the second panel 1112 and the third panel 1113, and the double door can be opened at any time to facilitate taking and placing of the reagent cartridge. The double door is provided with a sensor (not shown) for detecting the open/close state of the double door and controlling the movement of the reagent needle 141 (see below) in different states, thereby protecting the safety of a user.

The front base shell 111 further includes a display 1114, and the display 1114 is used for displaying test data and inputting external commands. In this embodiment, the display 1114 is fixed to the first panel 1111.

Referring to fig. 1 and 4, the front base shell 111 further includes a movable door 1115. The movable door 1115 is used for placing a keyboard and/or a mouse. In this embodiment, the movable door 1115 is located at the upper half of the first panel portion 1111. Specifically, the movable door 1115 is positioned between the display 1114 and the single-door.

Referring to fig. 1, the front base 111 further includes a chip inlet 1117 and a driver button 1118. The chip inlet 1117 faces the chip drive 170 for placing a sequencing chip. The chip driver button 1118 is located at one side of the chip inlet 1117, and the chip driver button 1118 is clicked to eject the chip inlet 1117. In this embodiment, the chip inlet 1117 and the chip driver key 1118 are located on the second panel portion 1112.

The front base shell 111 is further provided with a host power switch, the movable door lock catch, a USB interface (not shown), and the like.

Referring to fig. 1, a first opening 1131 and a first air inlet 1132 are formed on the first side base shell 113. The first opening 1131 is used for receiving and fixing the waste chip collecting assembly 190. First air intake 1132 is used for the air inlet to the heat dissipation.

Referring to fig. 2, a plurality of air outlets 1121 are further formed on the rear base housing 112. The air outlet 1121 is used for exhausting air and dissipating heat. The second side base shell 114 is formed with a second air inlet 1142, and the second air inlet 1142 is used for supplying air to dissipate heat.

Referring to fig. 2-3, a maintenance door 1141 is further formed on the second side base shell 114, and the maintenance door 1141 is close to the optical inspection assembly 150 to facilitate maintenance of the optical inspection assembly 150. Specifically, the maintenance door 1141 is connected to the rear base shell 112 by a hinge or a hinge, and the maintenance door 1141 can be opened and closed by 180 degrees to make up more maintenance space. An electronic lock 1143 is further fixed on the second side base shell 114, and the electronic lock 1143 is located at one side of the maintenance door 1141. The maintenance door 1141 is provided with an electronic lock bolt (not shown), a lock 1144 and a lock hole 1145. When the maintenance door 1141 is closed, the bolt of the electronic lock is inserted into the electronic lock 1143, and after the electronic lock 1143 senses the bolt of the electronic lock, the bolt of the electronic lock 1143 is buckled by the lock cylinder of the electronic lock. When the door opening authority is obtained, the electronic lock 1143 can be controlled to open through the operation of a software interface, and then the maintenance door 1141 can be opened by pulling the lock 1144 outwards. In case of power failure, if the maintenance door 1141 needs to be opened, a special key can be used to unlock the maintenance door 1141 mechanically through the lock hole 1145.

The bottom base 115 is further formed with a plurality of fixing legs 1151 and a plurality of casters (not shown). The securing feet 1151 are used to support the sequencer 100 to ensure that the sequencer 100 rests smoothly. The casters are used to move the sequencer 100.

Referring to fig. 5, a shaped groove 34 is further formed on the front base shell 111, and a light guide assembly 30 is fixed in the shaped groove 34. In the present embodiment, the profile groove 34 is located on the second panel 1112.

The light guide assembly 30 includes a light guide plate 31, a light guide post 32 and a light strip 33. The light guide plate 31 and the light guide post 32 are used for guiding light, and the light strip 33 is used for emitting light. The light guide plate 31, the light guide post 32 and the light strip 33 are accommodated in the irregular groove 34, the light guide plate 31 faces the outside, the light guide post 32 is fixed on the light guide plate 31, and the light strip 33 is attached to the light guide post 32. The light guide plate 31 is provided with an air inlet 311 for heat dissipation, and the air inlet 311, the light guide pillar 32 and the light strip 33 have the same shape and are identical to each other, so that the distance between light transmission paths is consistent, and the brightness of the light displayed outside is consistent. The surface treatment is performed between the light guide plate 31 and the light guide column 32, so that light cross and light leakage can be effectively prevented. The surface treatment comprises polishing treatment (corresponding to the light transmitting surface), frosting treatment (corresponding to the light inlet surface) and blacking treatment (corresponding to the light transmitting surface and the surface except the light inlet surface, and light leakage is prevented). In the present embodiment, the ventilation holes 311, the light guide bar 32, and the light strip 33 have a spiral shape, and look like a DNA double helix structure. The light guide assembly 30 realizes perfect combination of the air inlet hole and the light guide assembly, and can not only realize air inlet heat dissipation and obtain light patterns with consistent brightness, but also obtain beautiful appearance.

Referring to fig. 1, 4 and 6, the frame assembly 120 includes a plurality of frames 20, and the housing 110 is formed and wrapped on the frames 20. The movable door 1115 is movably connected to the frame 20 through two double-shaft rotating shaft blocks 10. Specifically, each of the two-axis rotation shaft blocks 10 includes a first rotation shaft 11 and a second rotation shaft 12. In the present embodiment, the first rotating shaft 11 and the second rotating shaft 12 are located on the same surface of the biaxial rotating shaft block 10. Two opposite surfaces of the two adjacent frames 20 are respectively provided with a first track hole 21 and a second track hole 22, the first track hole 21 is located above the second track hole 22, the first track hole 21 and the second track hole 22 are both arc-shaped, and the bending directions of the first track hole 21 and the second track hole 22 are opposite. The two-shaft rotation shaft block 10 is fixed to one end of the movable door 1115, the first rotation shaft 11 is accommodated in the first track hole 21, and the second rotation shaft 12 is accommodated in the second track hole 22. Pulling one end of the movable door 1115, which is far away from the two-axis rotation shaft block 10, so that the first rotation shaft 11 and the second rotation shaft 12 slide in the first track hole 21 and the second track hole 22, and the movable door 1115 is opened. When the movable door 1115 is pushed in the opposite direction, the first and

second shafts

11 and 12 slide in the first and second track holes 21 and 22 in the opposite direction, so as to close the movable door 1115.

Referring to fig. 8, the refrigeration device 130 is used to provide sequencing reagents (including buffers and washing solutions) required for gene sequencing to the corresponding biochemical platform assembly 140. The refrigerating device 130 includes a refrigerating chamber 40 and a refrigerating module 50 fixed at one end of the refrigerating chamber 40. The refrigeration cavity 40 is used for accommodating and refrigerating sequencing reagents, and the sequencing reagents are contained in a sequencing kit (not shown) which is placed in the refrigeration cavity 40. The refrigeration module 50 is configured to produce and flow cold gas within the refrigeration chamber 40 to refrigerate the sequencing reagents.

Specifically, referring to fig. 9, the refrigerating chamber 40 includes a housing layer 41, a heat insulation layer 42, a cold storage liner layer 43, a cold air circulation layer 44 and an inner layer 45. The cold air circulation layer 44 is formed outside the inner layer 45, the cold storage liner layer 43 is formed outside the cold air circulation layer 44, the heat insulation layer 42 is formed outside the cold storage liner layer 43, and the shell layer 41 is formed outside the heat insulation layer 42.

Referring to fig. 9-10, the cooling module 50 includes a TEC assembly 51, a cold air fin 52, a cold air fan 53, a heat sink 54, and a heat dissipation fan 55. The cold air fan 53 is located at one end of the cold storage cavity 40, the cold air fin 52 is fixed on the cold surface of the TEC assembly 51 and faces the cold air fan 53, the heat sink 54 is fixed on the TEC assembly 51, and the heat dissipation fan 55 is fixed at one end of the heat sink 54. The cooling fins 52 are vertically oriented. The TEC assembly 51 is used for producing cold air, the cold air is firstly transmitted to the cold air fin 52, the cold air fan 53 blows the cold air generated by the cold air fin 52 to flow into the cold air circulation layer 44 along the direction of the cold air fin 52, and the cold air flows from the cold air circulation layer 44 to the front part of the cold storage cavity 40, so that the whole cold storage cavity 40 is in a cold air atmosphere to better refrigerate the sequencing reagent.

Referring to fig. 6, one of the biochemical platform assemblies 140 is located above one of the refrigeration devices 130. The biochemical platform assembly 140 is used for performing gene sequencing reaction on the sample on the sequencing chip. Each of the biochemical platform assemblies 140 has a plurality of reagent needles 141, and the reagent needles 141 are inserted into the refrigerating cavity 40 of the refrigerating device 130 and contact the sequencing reagent to aspirate a reagent.

Referring to fig. 6, the optical detection assembly 150 is located at one side of the refrigeration device 130 and the biochemical platform assembly 140 and is used for optically detecting the chip after the gene sequencing reaction so as to collect data of the sample on the chip after the gene sequencing reaction.

Referring to FIG. 6, the robot assembly 160 is located at one side of the optical inspection assembly 150 and the biochemical platform assembly 140 and is used for transferring the sequencing chip between the optical inspection assembly 150 and the biochemical platform assembly 140 and transferring the sequencing chip into the waste chip collection assembly 190 after the sequencing is completed.

Referring to FIG. 6, the chip driver 170 faces the chip inlet 1117 and is used for receiving a sequencing chip from the chip inlet 1117. By depressing the driver key 1118, the driver 170 can be ejected from the chip inlet 1117. The robot assembly 160 grabs the sequencing chip from the chip drive 170 and transfers the sequencing chip to the biochemical platform assembly 140 for biochemical reaction.

Referring to fig. 1-2, 5 and 7, an air intake channel 101 and a heat dissipation channel 102 are further disposed inside the casing 110 of the sequencer 100, the air intake channel 101 corresponds to the first air inlet 1132, the second air inlet 1142 and the air inlet hole 311, and the heat dissipation channel 102 corresponds to the air outlet 1121, so as to dissipate heat.

Referring to fig. 11, the cleaning kit assembly 180 is located below the refrigerating device 130, and the cleaning kit assembly 180 is used for cleaning the pipeline, the sequencing chip, the biochemical platform assembly 140, and the like. The cleaning kit assembly 180 includes a cleaning kit 61 and a drawer 62. The washing reagent cartridge 61 is housed in the drawer 62. One end of the washing reagent box 61 is provided with a boss 611, a side wall of the drawer 62 is provided with a notch 621, and the boss 611 is accommodated in the notch 621. The sequencer 100 further includes a photoelectric sensor 70, the photoelectric sensor 70 is located in the casing 110 and is opposite to one end of the washing reagent box assembly 180, and the photoelectric sensor 70 is used for detecting whether the washing reagent box 61 is placed in or not and whether the drawer 62 is pushed to the right position or not. When the washing reagent kit 61 is placed in the drawer 62 and the drawer 62 is pushed to a designated position, the photoelectric sensor 70 is activated. When the washing reagent kit 61 is not put in or the drawer 62 (with the washing reagent kit 61) is not pushed to a specified position, the photoelectric sensor 70 is not activated.

Referring to fig. 6 and 12, the waste chip collecting assembly 190 is disposed at one side of the robot assembly 160 and the biochemical platform assembly 140 for collecting waste chips after sequencing. The waste chip collecting assembly 190 comprises a collecting bin door 191 and a waste chip collecting bin 192, the collecting bin door 191 is connected to the waste chip collecting bin 192 to push the lower part of the collecting bin door 191, and the collecting bin door 191 is opened to take out the waste chips. The collection door 191 is received within the first opening 1131. In this embodiment, the waste chip collecting assembly 190 further includes a shielding cover 194, the shielding cover 194 is fixed on the waste chip collecting chamber 192, a second opening 195 is formed in the middle of the shielding cover 194, and the waste chips enter the waste chip collecting chamber 192 through the second opening 195.

Of course, the sequencer 100 also includes electronic control components (not shown).

The sequencer 100 operates as follows:

first, the user clicks the chip drive button 1118, the chip drive 170 is ejected from the chip inlet 1117, the user puts the sequencing chip into the chip drive 170 and closes the chip drive 170, and the robot assembly 160 transfers the sequencing chip from the chip drive 170 to a designated position of the bio-platform assembly 140.

Next, the user places the kit into the cold storage device 130 and the wash kit assembly 180.

Again, sequencing is initiated, waiting, and the display screen 1114 displays sequencing process parameters.

Finally, the sequencer 100 outputs sequencing data.

The sequencer provided by the invention can 1) simultaneously carry out gene sequencing work of a plurality of sequencing chips without mutual interference, thereby improving the sequencing speed and reducing the cost; 2) the manipulator assembly is arranged, so that the automation degree of the sequencer can be improved, the sequencing speed and the sequencing flux are further improved, and the cost is reduced; 3) a photoelectric sensor is arranged at one end of the cleaning kit component, so that whether the cleaning kit is put in and whether the drawer is pushed in place can be detected; 4) the cold storage device comprises a cold storage cavity and a refrigeration module fixed at one end of the cold storage cavity, and can produce cold air and make the cold air flow in the cold storage cavity, so that the sequencing reagent can be refrigerated conveniently; 5) a movable door is connected to the shell by adopting a double-shaft rotating structure, so that the gap between the movable door and the shell caused by matching parts (such as a connecting piece and the like) can be reduced, and the attractiveness of the shell is enhanced; 6) the light guide assembly is combined with the air inlet hole, so that the lighting effect can be enhanced, and the heat can be dissipated.

Although the present invention has been described with reference to the above preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A sequencer, the sequencer comprising:

the biochemical platform component is used for generating gene sequencing reaction on a sample in the sequencing chip;

the optical detection component is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing;

the manipulator assembly is used for transferring the sequencing chip between the optical detection assembly and the biochemical platform assembly; after the biochemical platform assembly carries out biochemical reaction, the mechanical arm assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection.

2. The sequencer of claim 1, further comprising a refrigeration device coupled to said biochemical platform assembly for providing sequencing reagents to a corresponding said biochemical platform assembly.

3. The sequencer according to claim 2, wherein said cold storage device comprises a cold storage chamber and a refrigeration module secured to one end of said cold storage chamber; the cold storage cavity is used for accommodating and refrigerating sequencing reagents, the sequencing reagents are arranged in a sequencing reagent box, and the sequencing reagent box is placed in the cold storage cavity; the refrigeration module is used for making cold air flow in the refrigeration cavity body so as to refrigerate the sequencing reagent.

4. The sequencer of claim 2, further comprising a wash kit assembly for washing the tubing, sequencing chip, and biochemical platform assembly.

5. The sequencer according to claim 4, wherein said wash kit assembly comprises a wash kit and a drawer, said wash kit being received in said drawer, said wash kit having a boss at one end thereof, said drawer having a notch in a side wall thereof, said boss being received in said notch.

6. The sequencer according to claim 5, further comprising a photoelectric sensor disposed opposite to an end of the wash reagent cartridge assembly, wherein the photoelectric sensor is configured to detect whether the wash reagent cartridge is inserted and whether the drawer is pushed into place; when the washing reagent box is placed in the drawer and the drawer is pushed to a specified position, the photoelectric sensor is triggered.

7. The sequencer according to claim 1, further comprising a housing and a dual-axis rotary structure coupled to said housing, said dual-axis rotary structure comprising a movable door and two dual-axis spindle blocks, each of said dual-axis spindle blocks comprising a first spindle and a second spindle; the shell comprises two frames which are oppositely arranged; two adjacent surfaces of the two frames are respectively provided with a first track hole and a second track hole, the first track hole is positioned above the second track hole, the first track hole and the second track hole are both arc-shaped, and the bending directions of the first track hole and the second track hole are opposite; the double-shaft rotating shaft block is fixed at one end of the movable door, the first rotating shaft is accommodated in the first track hole, and the second rotating shaft is accommodated in the second track hole; pulling one end of the movable door, which is far away from the double-shaft rotating shaft block, and enabling the first rotating shaft and the second rotating shaft to slide in the first track hole and the second track hole so as to open the movable door; and pushing the movable door in the opposite direction, wherein the first rotating shaft and the second rotating shaft slide in the first track hole and the second track hole in the opposite direction to close the movable door.

8. The sequencer according to claim 7, wherein a groove is formed in the housing, the sequencer further comprising a light guide assembly, the light guide assembly comprising a light guide plate, a light guide post and a light strip, the light guide plate, the light guide post and the light strip being received in the groove, the light guide plate facing the outside, the light guide post being fixed to the light guide plate, the light strip being attached to the light guide post; the light guide plate is provided with an air inlet, the light guide column and the lamp strip are identical in shape, and the light guide column, the air inlet and the lamp strip are identical in shape.

9. The sequencer of claim 7, wherein said housing further defines a chip inlet for receiving a sequencing chip from the outside; the sequencer also comprises a chip driver, the chip driver is opposite to the chip inlet and is used for receiving the sequencing chip from the chip inlet, and the chip driver can be ejected out of the chip inlet; and the manipulator component grabs the sequencing chip from the core drive and transfers the sequencing chip to the biochemical platform component for biochemical reaction.

10. The sequencer according to claim 1, further comprising a waste chip collection assembly for collecting waste chips after sequencing; the manipulator assembly transfers the sequencing chip into the waste chip collecting assembly after the sequencing is finished; the waste chip collecting assembly comprises a collecting bin door and a waste chip collecting bin, wherein the collecting bin door is connected to the waste chip collecting bin and pushes the lower part of the collecting bin door, and the collecting bin door is opened to take out the waste chips.