CN112646700B

CN112646700B - Sequencer

Info

Publication number: CN112646700B
Application number: CN201910964855.9A
Authority: CN
Inventors: 黄立平; 孙磊林; 陈泽华; 余利; 陈文�; 李骏杨; 刘卓; 李开金
Original assignee: MGI Tech Co Ltd
Current assignee: MGI Tech Co Ltd
Priority date: 2019-10-11
Filing date: 2019-10-11
Publication date: 2024-05-17
Anticipated expiration: 2039-10-11
Also published as: CN112646700A

Abstract

A sequencer, the sequencer comprising: the biochemical platform component is used for carrying out gene sequencing reaction on a sample in the sequencing chip; the optical detection component is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing reaction; the manipulator assembly is used for transferring the sequencing chip between the optical detection assembly and the biochemical platform assembly; after biochemical reaction is carried out on the biochemical platform assembly, the manipulator assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection. The sequencer has the advantages of high flexibility, high sequencing speed and low sequencing cost.

Description

Sequencer

Technical Field

The invention relates to a sequencer.

Background

The gene sequencing technology is widely applied to whole genome sequencing, transcriptome sequencing, metagenome sequencing and the like at present, is a powerful tool for analyzing biochemical evolution and classification, researching diseases related to cancers, autism and the like, performing in-vitro diagnosis and the like, promotes people to further understand life science, and promotes the development of health industry.

In the prior art, a gene sequencer is often used to sequence genes in samples in a flow cell. The flow cell is also called a sequencing chip, which is a region for loading a sample for gene sequencing and for which a sequencing reaction occurs. Because of the non-repeatability of sample loading, and the need to avoid cross-contamination between different samples, sequencing chips are often designed for single use, repeatable installation and removal, and in a totally enclosed fashion. The lower surface of the sequencing chip is usually a substrate, and the gene sample to be tested can be fixed on the surface of the substrate through a biological or chemical reaction. Because the sequencing chip is transferred to the signal detection system for signal detection after the sequencing reaction, the sequencing reaction can be carried out again after the signal detection, or the transfer of the waste chip is completed manually after the sequencing is completed, and the common gene sequencer in the prior art can only operate one or two sequencing chips at the same time, so that the gene sequencer in the prior art has the problems of poor flexibility, low sequencing speed, high cost and the like.

Disclosure of Invention

In view of the above, the present invention provides a sequencer capable of solving the above-mentioned technical problems.

A sequencer, the sequencer comprising: the biochemical platform component is used for carrying out gene sequencing reaction on a sample in the sequencing chip; the optical detection component is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing; the manipulator assembly is used for transferring the sequencing chip between the optical detection assembly and the biochemical platform assembly; after biochemical reaction is carried out on the biochemical platform assembly, the manipulator assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection.

Further, the sequencer also comprises a refrigerating device, wherein the refrigerating device is connected with the biochemical platform assembly and is used for providing sequencing reagents required by gene sequencing reaction for the corresponding biochemical platform assembly.

Further, the refrigerating device comprises a refrigerating cavity and a refrigerating module fixed at one end of the refrigerating cavity; the refrigerating cavity is used for accommodating and refrigerating a sequencing reagent, the sequencing reagent is arranged in a sequencing kit, and the sequencing kit is arranged in the refrigerating cavity; the refrigeration module is used for manufacturing cold air to flow in the refrigeration cavity so as to refrigerate the sequencing reagent.

Further, the sequencer also comprises a cleaning kit component, wherein the cleaning kit component is used for cleaning the pipeline, the sequencing chip and the biochemical platform component.

Further, the cleaning kit assembly comprises a cleaning kit and a drawer, wherein the cleaning kit is accommodated in the drawer, one end of the cleaning kit is provided with a boss, one side wall of the drawer is provided with a notch, and the boss is accommodated in the notch.

Further, the sequencer also comprises a photoelectric sensor, wherein the photoelectric sensor is opposite to one end of the cleaning kit component and is used for detecting whether the cleaning kit is put in place or not and whether the drawer is pushed in place or not; the photo sensor is triggered when the wash kit is placed in the drawer, which is pushed to a designated position.

Further, the sequencer also comprises a shell and a double-shaft rotating structure connected to the shell, wherein the double-shaft rotating structure comprises a movable door and two double-shaft rotating shaft blocks, and each double-shaft rotating shaft block comprises a first rotating shaft and a second rotating shaft; the shell comprises two frames which are oppositely arranged; a first track hole and a second track hole are respectively formed in two adjacent surfaces of the two frames, the first track hole is positioned above the second track hole, and the first track hole and the second track hole are arc-shaped and have opposite bending directions; the double-shaft rotating shaft block is fixed at one end of the movable door, the first rotating shaft is accommodated in the first track hole, and the second rotating shaft is accommodated in the second track hole; pulling one end of the movable door far away from the double-shaft rotating shaft block, wherein the first rotating shaft and the second rotating shaft slide in the first track hole and the second track hole so as to open the movable door; the movable door is pushed in the opposite direction, and the first rotating shaft and the second rotating shaft slide in the opposite direction in the first track hole and the second track hole so as to close the movable door.

Further, a special-shaped groove is formed in the shell, the sequencer further comprises a light guide assembly, the light guide assembly comprises a light guide plate, a light guide column and a light belt, the light guide plate, the light guide column and the light belt are contained in the special-shaped groove, the light guide plate faces to the outside, the light guide column is fixed on the light guide plate, and the light belt is attached to the light guide column; the light guide plate is provided with an air inlet, the light guide column and the lamp strip are identical in shape, and the light guide column, the air inlet and the lamp strip are identical in shape.

Further, a chip inlet is further formed in the shell, and the chip inlet is used for placing a sequencing chip from the outside; the sequencer also comprises a core driver, wherein the core driver is opposite to the chip inlet and is used for receiving a sequencing chip from the chip inlet, and the core driver can be ejected from the chip inlet; the manipulator assembly grabs the sequencing chip from the core driver and transfers the sequencing chip to the biochemical platform assembly for biochemical reaction.

Further, the sequencer also comprises a waste chip collecting assembly, wherein the waste chip collecting assembly is used for collecting the waste chips after sequencing; the manipulator assembly transfers the sequencing chip into the waste chip collection assembly after sequencing is completed; the waste chip collecting assembly comprises a collecting bin gate and a waste chip collecting bin, wherein the collecting bin gate is connected to the waste chip collecting bin and pushes the lower part of the collecting bin gate, and the collecting bin gate is opened to take out the waste chip.

The sequencer provided by the invention 1) can simultaneously perform gene sequencing work of a plurality of sequencing chips, and the sequencing chips are not mutually interfered, so that the sequencing speed can be increased, and the cost is reduced; 2) The mechanical arm assembly is arranged, so that the degree of automation of the sequencer can be improved, the sequencing speed and flux are further improved, and the cost is reduced; 3) A photoelectric sensor is arranged at one end of the cleaning kit assembly and can detect whether the cleaning kit is put in place or not and whether the drawer is pushed in place or not; 4) The refrigerating device comprises a refrigerating cavity and a refrigerating module fixed at one end of the refrigerating cavity, and can be used for producing cold air and flowing the cold air in the refrigerating cavity, thereby being beneficial to refrigerating the test reagent; 5) The double-shaft rotating structure is adopted to connect the movable door on the shell, so that the gap between the movable door and the shell caused by matched parts (such as connecting pieces and the like) can be reduced, and the aesthetic degree of the shell is enhanced; 6) The light guide assembly is combined with the air inlet, so that the light efficiency can be enhanced, and the heat can be dissipated.

Drawings

Fig. 1 is a schematic diagram of a three-dimensional structure of a sequencer according to the present invention.

FIG. 2 is a schematic perspective view of the sequencer shown in FIG. 1 in another orientation.

FIG. 3 is a schematic diagram of a maintenance door in the sequencer shown in FIG. 1.

FIG. 4 is a schematic structural view of a biaxial rotation structure and a partial enlarged view of the biaxial rotation structure in the sequencer shown in FIG. 1.

Fig. 5 is a schematic structural diagram of a light guide assembly in the sequencer shown in fig. 1.

FIG. 6 is a schematic perspective view of the sequencer shown in FIG. 1 with the housing removed.

FIG. 7 is another reverse perspective view of the sequencer shown in FIG. 1 with the housing removed, as shown in FIG. 6.

Fig. 8 is a schematic perspective view of a refrigerator in the sequencer shown in fig. 6.

Fig. 9 is a cross-sectional view of the refrigeration unit of fig. 8 taken along section line IX-IX.

Fig. 10 is a schematic perspective view of a refrigerating module in the refrigerating apparatus shown in fig. 9.

FIG. 11 is a schematic perspective view of a cleaning kit assembly in the sequencer shown in FIG. 6.

FIG. 12 is a schematic perspective view of a waste chip collection assembly in the sequencer shown in FIG. 6.

Description of the main reference signs

Sequencer 100

Housing 110

Front base shell 111

First panel section 1111

Second panel portion 1112

Third panel 1113

Display 1114

Movable door 1115

Single door 1116

Chip inlet 1117

Core drive key 1118

Rear base shell 112

Air outlet 1121

First side-group housing 113

First opening 1131

First air inlet 1132

Second side-group casing 114

Maintenance door 1141

Second air inlet 1142

Electronic lock 1143

Lock 1144

Lock hole 1145

Bottom base shell 115

Fixed leg 1151

Frame 20

First track hole 21

Second track hole 22

Double-shaft rotating shaft block 10

First rotating shaft 11

Second rotating shaft 12

Light guide assembly 30

Light guide plate 31

Air inlet 311

Light guide column 32

Lamp strip 33

Profiled groove 34

Frame assembly 120

Refrigerating device 130

Refrigerating chamber 40

Shell layer 41

Insulation layer 42

Cold storage liner layer 43

Cold air circulation layer 44

Inner layer 45

Refrigeration module 50

TEC assembly 51

Cold air fin 52

Cold air fan 53

Radiator 54

Heat radiation fan 55

Biochemical platform assembly 140

Reagent needle 141

Optical detection assembly 150

Manipulator assembly 160

Core drive 170

Cleaning kit assembly 180

Cleaning kit 61

Boss 611

Drawer 62

Notch 621

Photoelectric sensor 70

Waste chip collection assembly 190

Collecting bin gate 191

Waste chip collecting bin 192

Shield 194

Second opening 195

Air intake channel 101

Radiating channel 102

The invention will be further described in the following detailed description in conjunction with the above-described figures.

Detailed Description

The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 12 of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Referring to fig. 1, a sequencer 100 according to a preferred embodiment of the present invention is provided, and the sequencer 100 is used for analyzing biochemical substances (gene sequences). The sequencer 100 includes a housing 110, a frame assembly 120, four refrigerators 130, four biochemical platform assemblies 140, an optical detection assembly 150, a robot assembly 160, a cartridge drive 170, four cleaning kit assemblies 180, and a waste chip collection assembly 190. The housing 110 is fixed to and wrapped around the frame assembly 120, and the refrigerator 130, the biochemical platform assembly 140, the optical detection assembly 150, the manipulator assembly 160, the cartridge 170, the cleaning kit assembly 180, and the waste chip collection assembly 190 are housed in the frame assembly 120.

In other embodiments, the number of the refrigerating device 130, the biochemical platform assembly 140, and the cleaning kit assembly 180 is not limited to 4, but may be 1,2, 3, or more than 4.

In this embodiment, the housing 110 includes a front base shell 111, a rear base shell 112 opposite to the front base shell 111, a first side base shell 113 connected to the front base shell 111 and the rear base shell 112, a second side base shell 114 opposite to the first side base shell 113, and a bottom base shell 115 connecting the front base shell 111, the rear base shell 112, the first side base shell 113, and the second side base shell 114. Of course, in other embodiments, the housing 110 further includes a top base housing (not numbered) opposite the bottom base housing 115.

In this embodiment, the front base shell 111 includes a first panel 1111, a second panel 1112, and a third panel 1113. The second panel 1112 is located between the first panel 1111 and the third panel 1113.

In this embodiment, the upper half parts of the first panel 1111, the second panel 1112 and the third panel 1113 may be designed to be a single door or a double door, and may be locked by an electronic lock (not shown), and may be unlocked and opened only when the sequencer 100 runs out of order, thereby facilitating maintenance. The lower half of the first panel 1111 is a single door 1116, and one end of the single door 1116 is fixed to the frame assembly 120. One end of the single door 1116 is connected to the frame assembly 120 by a hinge or a hinge, etc., and the other end is fixed to the frame assembly 120 by a screw. When the single door 1116 needs to be opened, the single door 1116 may be opened by rotating the single door 1116 only by removing or unscrewing the screw using a tool. The screw fixing is used for safety to prevent the user from opening the single door 1116 at his or her discretion.

In this embodiment, a double door may be formed between the second panel 1112 and the lower half of the third panel 1113, and may be opened at any time to facilitate taking and placing of the kit. The double door is provided with a sensor (not shown) for detecting the open/close state of the double door and controlling the movement of the reagent needle 141 (see below) in different states, thereby protecting the safety of the user.

The front base shell 111 further includes a display 1114, where the display 1114 is used to display test data and input external instructions. In this embodiment, the display 1114 is fixed to the first panel 1111.

Referring to fig. 1 and 4, the front base housing 111 further includes a movable door 1115. The movable door 1115 is used for placing a keyboard and/or a mouse. In the present embodiment, the sliding door 1115 is located at the upper half of the first panel 1111. Specifically, the movable door 1115 is positioned between the display 1114 and the single door.

Referring to fig. 1, the front base shell 111 further includes a chip inlet 1117 and a core driver 1118. The chip inlet 1117 is opposite the core driver 170 for placement into a sequencing chip. The core drive key 1118 is located on one side of the chip inlet 1117 and the core drive key 1118 is clicked to eject the chip inlet 1117. In this embodiment, the chip inlet 1117 and the core driver key 1118 are located on the second panel 1112.

The front base shell 111 is further provided with a host power switch, the sliding door lock catch, a USB interface (not shown), and the like.

Referring to fig. 1, the first side housing 113 has a first opening 1131 and a first air inlet 1132. The first opening 1131 is configured to receive and retain the waste chip collection assembly 190. The first air inlet 1132 is used for air intake to dissipate heat.

Referring to fig. 2, a plurality of air outlets 1121 are further formed in the rear base housing 112. The air outlet 1121 is used for exhausting and dissipating heat. The second side housing 114 has a second air inlet 1142 formed thereon, and the second air inlet 1142 is used for air intake to dissipate heat.

Referring to fig. 2-3, a maintenance door 1141 is further formed on the second side housing 114, and the maintenance door 1141 is close to the optical detection assembly 150, so as to facilitate maintenance of the optical detection assembly 150. Specifically, the maintenance door 1141 is connected to the rear base housing 112 by a hinge or a hinge, etc., and the maintenance door 1141 may be opened and closed by 180 degrees to make more maintenance space available. An electronic lock 1143 is also secured to the second side housing 114, the electronic lock 1143 being located on one side of the service door 1141. The maintenance door 1141 is provided with an electronic lock tongue (not shown), a lock 1144, and a lock hole 1145. When the maintenance door 1141 is closed, the electronic lock tongue is inserted into the electronic lock 1143, and when the electronic lock 1143 senses the electronic lock tongue, the lock core of the electronic lock 1143 locks the electronic lock tongue. When the door opening authority is obtained, the electronic lock 1143 is controlled to be opened by operating on a software interface, and then the maintenance door 1141 is opened by pulling the lock 1144 outwards. In the event of a power outage, if the service door 1141 is to be opened, a special key may be used to effect a mechanical unlocking through the lock aperture 1145, opening the service door 1141.

The bottom base housing 115 further has a plurality of fixed legs 1151 and a plurality of casters (not shown). The fixed feet 1151 are used to support the sequencer 100 to ensure that the sequencer 100 sits smoothly. The casters are used to move the sequencer 100.

Referring to fig. 5, the front base shell 111 further has a shaped groove 34, and a light guide assembly 30 is fixed in the shaped groove 34. In the present embodiment, the profiled groove 34 is located on the second panel portion 1112.

The light guide assembly 30 includes a light guide plate 31, a light guide column 32 and a light strip 33. The light guide plate 31 and the light guide posts 32 are used for guiding light, and the lamp strip 33 is used for emitting light. The light guide plate 31, the light guide column 32 and the light strip 33 are accommodated in the special-shaped groove 34, the light guide plate 31 faces the outside, the light guide column 32 is fixed on the light guide plate 31, and the light strip 33 is attached to the light guide column 32. The light guide plate 31 is provided with an air inlet 311 for heat dissipation, and the air inlet 311, the light guide column 32 and the light strip 33 are identical in shape and identical in shape, so that the distance of the light transmission path is consistent, and the brightness consistency of the light outside is ensured. The surface treatment is performed between the light guide plate 31 and the light guide columns 32, so that light leakage and light crosstalk can be effectively prevented. Wherein, the surface treatment comprises polishing treatment (corresponding to the light-transmitting surface), frosting treatment (corresponding to the light-entering surface) and blackening treatment (corresponding to the light-transmitting surface and the surface outside the light-entering surface, and preventing light leakage). In the present embodiment, the air inlet 311, the light guide pole 32, and the lamp strip 33 are spiral, and look like a DNA double spiral structure. The light guide assembly 30 realizes perfect combination of the air inlet and the light guide assembly, so that air can be introduced and radiated, light patterns with consistent brightness can be obtained, and attractive appearance can be obtained.

Referring to fig. 1,4 and 6, the frame assembly 120 includes a plurality of frames 20, and the housing 110 is formed and wrapped around the frames 20. The movable door 1115 is movably connected to the frame 20 through two dual-axis rotating blocks 10. Specifically, each biaxial rotation shaft block 10 includes a first rotation shaft 11 and a second rotation shaft 12. In the present embodiment, the first shaft 11 and the second shaft 12 are located on the same surface of the biaxial rotation block 10. Two opposite surfaces of the two adjacent frames 20 are respectively provided with a first track hole 21 and a second track hole 22, the first track hole 21 is positioned above the second track hole 22, and the first track hole 21 and the second track hole 22 are arc-shaped and have opposite bending directions. The dual-shaft rotating shaft block 10 is fixed at one end of the movable door 1115, the first rotating shaft 11 is accommodated in the first track hole 21, and the second rotating shaft 12 is accommodated in the second track hole 22. Pulling one end of the movable door 1115 away from the dual-axis rotation block 10, the first and second rotation shafts 11 and 12 slide in the first and second track holes 21 and 22 to open the movable door 1115. The movable door 1115 is pushed in the opposite direction, and the first rotating shaft 11 and the second rotating shaft 12 slide in the opposite direction in the first track hole 21 and the second track hole 22 to close the movable door 1115.

Referring to fig. 8, the refrigerator 130 is configured to provide sequencing reagents (including buffer and cleaning solution, etc.) required for gene sequencing to the corresponding biochemical platform assembly 140. The refrigeration device 130 includes a refrigeration cavity 40 and a refrigeration module 50 fixed at one end of the refrigeration cavity 40. The refrigerated cavity 40 is configured to house and refrigerated sequencing reagents, the sequencing reagents being contained within a sequencing kit (not shown) that is disposed within the refrigerated cavity 40. The cooling module 50 is used to produce cool air and flow the cool air within the cooling chamber 40 for cooling the sequencing reagents.

Specifically, referring to fig. 9, the refrigerating chamber 40 includes a housing layer 41, a heat-preserving layer 42, a cold-storage liner layer 43, a cold-air circulation layer 44 and an inner layer 45. The cold air circulation layer 44 is formed outside the inner layer 45, the cold storage liner layer 43 is formed outside the cold air circulation layer 44, the heat insulation layer 42 is formed outside the cold storage liner layer 43, and the shell layer 41 is formed outside the heat insulation layer 42.

Referring to fig. 9-10, the refrigeration module 50 includes a TEC assembly 51, a cooling air fin 52, a cooling air fan 53, a heat sink 54, and a heat dissipation fan 55. The cold air fan 53 is located at one end of the refrigeration cavity 40, the cold air fins 52 are fixed on the cold surface of the TEC assembly 51 and face the cold air fan 53, the radiator 54 is fixed on the TEC assembly 51, and the radiator fan 55 is fixed at one end of the radiator 54. The cooling fins 52 are vertically oriented. The TEC assembly 51 is used for manufacturing cold air, the cold air is firstly transferred to the cold air fins 52, the cold air generated by blowing the cold air fins 52 by the cold air fan 53 flows into the cold air circulation layer 44 along the direction of the cold air fins 52, and the cold air flows from the cold air circulation layer 44 to the front part of the cold storage cavity 40, so that the whole cold storage cavity 40 is in a cold air atmosphere to better refrigerate the sequencing reagent.

Referring to fig. 6, one of the biochemical platform assemblies 140 is located above one of the refrigerating apparatuses 130. The biochemical platform assembly 140 is used for performing a gene sequencing reaction on a sample on a sequencing chip. Each of the biochemical platform assemblies 140 has a plurality of reagent pins 141, and the reagent pins 141 are inserted into the refrigerating chamber 40 of the refrigerating device 130 and contact the sequencing reagent to aspirate the reagent.

Referring to fig. 6, the optical detection assembly 150 is located at one side of the cold storage device 130 and the biochemical platform assembly 140 and is used for optically detecting the chip after the gene sequencing reaction to collect the data of the sample on the sequencing chip after the gene sequencing reaction.

Referring to fig. 6, the manipulator assembly 160 is located at one side of the optical detection assembly 150 and the biochemical platform assembly 140, and is used for transferring the sequencing chip between the optical detection assembly 150 and the biochemical platform assembly 140, and transferring the sequencing chip into the waste chip collection assembly 190 after sequencing is completed.

Referring to FIG. 6, the chip driver 170 is facing the chip inlet 1117 and is configured to receive a sequencing chip from the chip inlet 1117. By pressing the core driver key 1118, the core driver 170 can be ejected from the chip inlet 1117. The manipulator assembly 160 grabs the sequencing chip from the core drive 170 and transfers the sequencing chip to the biochemical platform assembly 140 for biochemical reaction.

Referring to fig. 1-2, fig. 5 and fig. 7, an air inlet channel 101 and a heat dissipation channel 102 are further disposed in the housing 110 of the sequencer 100, the air inlet channel 101 corresponds to the first air inlet 1132, the second air inlet 1142 and the air inlet 311, and the heat dissipation channel 102 corresponds to the air outlet 1121, so as to facilitate heat dissipation.

Referring to fig. 11, the cleaning kit assembly 180 is located below the refrigerating device 130, and the cleaning kit assembly 180 is used for cleaning the pipeline, the sequencing chip, the biochemical platform assembly 140, and the like. The cleaning kit assembly 180 includes a cleaning kit 61 and a drawer 62. The cleaning kit 61 is accommodated in the drawer 62. One end of the cleaning kit 61 has a boss 611, and a side wall of the drawer 62 has a notch 621, and the boss 611 is accommodated in the notch 621. The sequencer 100 further comprises a photo sensor 70, wherein the photo sensor 70 is disposed in the housing 110 and opposite to one end of the cleaning kit assembly 180, and the photo sensor 70 is used for detecting whether the cleaning kit 61 is put in place or not and whether the drawer 62 is pushed in place or not. When the cleaning kit 61 is placed in the drawer 62, the drawer 62 is pushed to a designated position, and the photo sensor 70 is triggered. When the washing reagent cartridge 61 is not put in or the drawer 62 (with the washing reagent cartridge 61 put therein) is not pushed to a designated position, the photo sensor 70 is not triggered.

Referring to fig. 6 and 12, the waste chip collecting assembly 190 is located at one side of the robot assembly 160 and the biochemical platform assembly 140, and is used for collecting the waste chips after sequencing. The waste chip collecting assembly 190 comprises a collecting bin gate 191 and a waste chip collecting bin 192, wherein the collecting bin gate 191 is connected to the waste chip collecting bin 192 to push the lower part of the collecting bin gate 191, the collecting bin gate 191 is opened, and the waste chip is taken out. The collection door 191 is received in the first opening 1131. In this embodiment, the waste chip collecting assembly 190 further includes a shielding cover 194, the shielding cover 194 is fixed on the waste chip collecting bin 192, a second opening 195 is formed in a middle portion of the shielding cover 194, and the waste chip enters the waste chip collecting bin 192 from the second opening 195.

Of course, the sequencer 100 also includes an electronic control assembly (not shown).

The operation method of the sequencer 100 is as follows:

First, the user clicks the core driver button 1118, the core driver 170 pops up from the chip inlet 1117, the user puts the sequencing chip into the core driver 170 and closes the core driver 170, and the manipulator assembly 160 transfers the sequencing chip from the core driver 170 to the designated position of the biochemical platform assembly 140.

Next, the user places the kit into the refrigerator 130 and washes the kit assembly 180.

Again, sequencing is started, waiting, the display 1114 displays sequencing process parameters.

Finally, the sequencer 100 outputs sequencing data.

The sequencer provided by the invention 1) can simultaneously perform gene sequencing work of a plurality of sequencing chips, and the sequencing chips are not mutually interfered, so that the sequencing speed can be improved, and the cost is reduced; 2) The mechanical arm assembly is arranged, so that the degree of automation of the sequencer can be improved, the sequencing speed and flux are further improved, and the cost is reduced; 3) A photoelectric sensor is arranged at one end of the cleaning kit assembly and can detect whether the cleaning kit is put in place or not and whether the drawer is pushed in place or not; 4) The refrigerating device comprises a refrigerating cavity and a refrigerating module fixed at one end of the refrigerating cavity, and can be used for producing cold air and flowing the cold air in the refrigerating cavity, thereby being beneficial to refrigerating the sequencing reagent; 5) The double-shaft rotating structure is adopted to connect the movable door on the shell, so that the gap between the movable door and the shell caused by matched parts (such as connecting pieces and the like) can be reduced, and the aesthetic degree of the shell is enhanced; 6) The light guide assembly is combined with the air inlet, so that the light efficiency can be enhanced, and the heat can be dissipated.

The present invention is not limited to the above-mentioned embodiments, but is capable of other and obvious modifications and equivalents of the above-mentioned embodiments, which will be apparent to those skilled in the art from consideration of the present invention without departing from the scope of the present invention.

Claims

1. A sequencer, the sequencer comprising:

each biochemical platform assembly is used for enabling a sample in the sequencing chip to generate a gene sequencing reaction;

The optical detection assembly is positioned at one side of the four biochemical platform assemblies and is used for carrying out optical detection on the chip subjected to the gene sequencing reaction so as to acquire data of the sequencing chip subjected to the gene sequencing;

A manipulator assembly for transferring the sequencing chip between the optical detection assembly and each of the biochemical platform assemblies; after biochemical reaction is carried out on the biochemical platform assembly, the manipulator assembly transfers the sequencing chip from the biochemical platform assembly to the optical detection assembly for optical detection;

The biochemical platform assemblies are positioned above the refrigerating devices, and each refrigerating device is connected with one biochemical platform assembly and used for providing sequencing reagents for the corresponding biochemical platform assembly;

The four cleaning kit components are positioned below the refrigerating device and are used for cleaning the pipeline, the sequencing chip and the biochemical platform component;

A core drive; and

A waste chip collection assembly.

2. The sequencer according to claim 1, wherein said refrigeration device comprises a refrigeration cavity and a refrigeration module secured to one end of said refrigeration cavity; the refrigerating cavity is used for accommodating and refrigerating a sequencing reagent, the sequencing reagent is arranged in a sequencing kit, and the sequencing kit is arranged in the refrigerating cavity; the refrigeration module is used for manufacturing cold air to flow in the refrigeration cavity so as to refrigerate the sequencing reagent.

3. The sequencer according to claim 1, wherein said cleaning kit assembly comprises a cleaning kit and a drawer, said cleaning kit being received in said drawer, one end of said cleaning kit having a boss, a side wall of said drawer having a notch, said boss being received in said notch.

4. The sequencer of claim 3, further comprising a photo sensor positioned opposite one end of said wash kit assembly, said photo sensor being adapted to detect whether said wash kit is in place and said drawer is pushed in place; the photo sensor is triggered when the wash kit is placed in the drawer, which is pushed to a designated position.

5. The sequencer according to claim 1, further comprising a housing and a dual-axis rotating structure coupled to said housing, said dual-axis rotating structure comprising a movable door and two dual-axis rotating blocks, each of said dual-axis rotating blocks comprising a first axis of rotation and a second axis of rotation; the shell comprises two frames which are oppositely arranged; a first track hole and a second track hole are respectively formed in two adjacent surfaces of the two frames, the first track hole is positioned above the second track hole, and the first track hole and the second track hole are arc-shaped and have opposite bending directions; the double-shaft rotating shaft block is fixed at one end of the movable door, the first rotating shaft is accommodated in the first track hole, and the second rotating shaft is accommodated in the second track hole; pulling one end of the movable door far away from the double-shaft rotating shaft block, wherein the first rotating shaft and the second rotating shaft slide in the first track hole and the second track hole so as to open the movable door; the movable door is pushed in the opposite direction, and the first rotating shaft and the second rotating shaft slide in the opposite direction in the first track hole and the second track hole so as to close the movable door.

6. The sequencer according to claim 5, wherein a special-shaped groove is formed in the housing, the sequencer further comprises a light guide assembly, the light guide assembly comprises a light guide plate, a light guide column and a light strip, the light guide plate, the light guide column and the light strip are accommodated in the special-shaped groove, the light guide plate faces to the outside, the light guide column is fixed on the light guide plate, and the light strip is attached to the light guide column; the light guide plate is provided with an air inlet, the light guide column and the lamp strip are identical in shape, and the light guide column, the air inlet and the lamp strip are identical in shape.

7. The sequencer according to claim 5, wherein said housing is further provided with a chip inlet for placing a sequencing chip from outside; the chip driver is opposite to the chip inlet and is used for receiving a sequencing chip from the chip inlet, and the chip driver can be ejected from the chip inlet; the manipulator assembly grabs the sequencing chip from the core driver and transfers the sequencing chip to the biochemical platform assembly for biochemical reaction.

8. The sequencer according to claim 1, wherein said spent chip collection assembly is configured to collect sequenced spent chips; the manipulator assembly transfers the sequencing chip into the waste chip collection assembly after sequencing is completed; the waste chip collecting assembly comprises a collecting bin gate and a waste chip collecting bin, wherein the collecting bin gate is connected to the waste chip collecting bin and pushes the lower part of the collecting bin gate, and the collecting bin gate is opened to take out the waste chip.