CN112646692A - Intestinal canal bacterium liquid culture apparatus - Google Patents

Intestinal canal bacterium liquid culture apparatus Download PDF

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Publication number
CN112646692A
CN112646692A CN202011459915.0A CN202011459915A CN112646692A CN 112646692 A CN112646692 A CN 112646692A CN 202011459915 A CN202011459915 A CN 202011459915A CN 112646692 A CN112646692 A CN 112646692A
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Prior art keywords
culture
sealing
liquid
rod
rotation axis
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CN202011459915.0A
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Chinese (zh)
Inventor
邱远
孙一鸣
巴桑旺堆
马海涛
杨桦
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Second Affiliated Hospital Army Medical University
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Second Affiliated Hospital Army Medical University
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Priority to CN202011459915.0A priority Critical patent/CN112646692A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • C12M27/06Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of culture equipment, in particular to an intestinal bacteria liquid culture device; comprises a culture shell, a sealing mechanism and a stirring mechanism. A mounting groove is formed in one side wall of the culture shell, and a rotating hole communicated with the outside is formed in the mounting groove; the sealing mechanism is hermetically sleeved on the culture shell; rabbling mechanism includes carousel, rotation axis and puddler, the carousel rotate install in the mounting groove, the rotatable seal installation of rotation axis in the rotation hole, and keeping away from of rotation axis one end portion of carousel stretches out rotatory hole, a tip fixed mounting of puddler in on the carousel, another tip of puddler is to keeping away from the one end of rotation axis extends, and with the lateral wall of cultivateing the casing rotates and is connected. The invention aims to solve the problem that when intestinal bacteria liquid is cultured, a culture device needs to be opened for stirring, so that the intestinal bacteria liquid is polluted.

Description

Intestinal canal bacterium liquid culture apparatus
Technical Field
The invention relates to the technical field of culture equipment, in particular to an intestinal tract bacteria liquid culture device.
Background
The gastrointestinal tract of a human is inhabited by a large variety of microorganisms, which are called intestinal flora, the intestinal flora is combined according to a certain proportion to form an ecological balance in quality and quantity, and medical staff are required to culture the intestinal flora when the intestinal tract of the patient is treated.
At present, when an intestinal bacteria liquid culture device is used for culture, fresh excrement and physiological saline are mixed in proportion to prepare turbid liquid, then the excrement turbid liquid and an anaerobic culture solution are mixed in proportion, finally the mixture is mixed with medicines in proportion and then is filled into the intestinal bacteria liquid culture device at a proper temperature for anaerobic culture, and in the culture process, workers need to perform operations such as stirring, sampling detection and the like.
However, current culture dish has just simply cooperated a lid, and the staff need open the lid and stir and sample the culture solution and detect, and when the lid was opened, the inside culture solution of culture dish just can contact with the air for a long time, and then causes the pollution to culture solution, leads to the condition of remaining culture solution just can't be fine use.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an intestinal bacteria liquid culture device, which aims to solve the problem that the intestinal bacteria liquid is polluted because the culture device needs to be opened for stirring when the intestinal bacteria liquid is cultured.
In order to achieve the purpose, the invention adopts the following technical scheme: an intestinal canal bacterial liquid culture device includes:
the culture shell is provided with a mounting groove on one side wall, and the mounting groove is provided with a rotating hole communicated with the outside;
the sealing mechanism is hermetically sleeved on the culture shell; and
rabbling mechanism, including carousel, rotation axis and puddler, the carousel rotate install in the mounting groove, the rotatable seal installation of rotation axis in the rotation hole, and keeping away from of rotation axis one end portion of carousel stretches out rotatory hole, a tip fixed mounting of puddler in on the carousel, another tip of puddler is to keeping away from the one end of rotation axis extends, and with the lateral wall of cultivateing the casing rotates and is connected.
When the technical scheme is adopted, after the sealing mechanism is opened, a mixed object used for culturing the intestinal canal bacterium liquid is added into the culture shell, and the culture shell is sealed through the sealing mechanism, so that the mixed object is cultured in the culture shell in a sealing manner. Rotate the rotation axis, the rotation axis drives the carousel and rotates to drive the puddler through the carousel and rotate, make the puddler stir the intestinal fungus liquid of cultivateing in the casing, thereby realize cultivateing that can be better under the casing resealing state stirs the fungus liquid of cultivateing, avoid the intestinal fungus liquid to receive the pollution.
Preferably, the sealing mechanism comprises a sealing cover body, a sealing gasket and a carrying handle, the sealing cover body covers the top of the culture shell, the sealing gasket is fixedly installed at the bottom of the sealing cover body, the sealing gasket is slidably and hermetically inserted into the opening of the culture shell, and the carrying handle is fixedly installed at the top of the sealing cover body.
The sealed lid closes and carries out preliminary sealed at the top of cultivateing the casing, is carrying out sealed once more to cultivateing the casing through sealed the pad to further strengthened the sealed effect of cultivateing the casing, mention the carrying handle, thereby be convenient for remove sealed lid or cultivate the casing.
Preferably, the stirring mechanism further comprises a motor and a belt, the motor is fixedly installed outside the culture shell, and the belt is connected with the rotating shaft of the motor and the rotating shaft in a tensioning manner respectively.
The motor is started, the motor drives the belt to rotate, and the rotating shaft is driven to rotate through the belt, so that the rotary table and the stirring rod rotate.
Preferably, the sampling device also comprises a sampling mechanism, wherein the sampling mechanism comprises a sealing plate, a spring, a push rod, a pull rod, a sliding plate and a pulling piece; cavities are formed in the stirring rod and the turntable, a liquid inlet communicated with the culture shell is formed in the cavity in the stirring rod, a plurality of liquid discharging holes are formed in the cavity in the turntable in the circumferential direction, a sliding channel communicated with the inside of the turntable is axially formed in the rotating shaft, a liquid discharging hole communicated with the outside is formed in the bottom of the mounting groove, and the liquid discharging hole can be communicated with the liquid discharging holes; the sealing plate is hinged to the stirring rod and can open or close the liquid inlet in a sealing manner, the pull rod is slidably mounted inside the stirring rod, the top of the push rod is hinged to the bottom of the sealing plate, which is far away from the hinged end of the sealing plate, the bottom of the push rod is hinged to the pull rod, the spring is sleeved on the pull rod, and two ends of the spring are fixedly connected with the surface of the pull rod and the inside of the stirring rod respectively; the sliding plate is arranged inside the rotary disc in a sealing mode along the axial direction of the rotating shaft in a sliding mode, the rotating shaft is fixedly connected with the end portion of the pull rod, the pulling piece is arranged in the sliding channel in a sliding mode, one end portion of the pulling piece is fixedly connected with the sealing plate, the other end portion of the pulling piece extends out of the sliding channel, the spring is in a natural state, the sealing plate seals the liquid inlet, and the sliding plate seals the liquid discharge hole.
When taking a sample to the intestinal fungus liquid, outside pulling piece, pulling piece drives sliding plate and pull rod slip in proper order, and then makes the push rod remove, spring compression, the push rod drives the cavity removal of closing plate to the puddler, thereby make the intestinal fungus liquid in the culture shell pass through the inlet and enter into the cavity, the intestinal fungus liquid passes through the outage again and enters into in the liquid outlet, make the intestinal fungus liquid discharge from the liquid outlet, thereby the staff of being convenient for realizes taking a sample to the intestinal fungus liquid in the culture shell.
Preferably, the sampling mechanism still includes sampling bucket and communicating pipe, sampling bucket detachable install in cultivate on the casing, and be located go out the bottom in liquid hole, the both ends of communicating pipe respectively with go out the liquid hole with sampling bucket screw seal communicates.
Go out the discharged intestinal fungus liquid of liquid hole and enter into in the sampling bucket through communicating pipe to concentrate the collection through the sampling bucket to intestinal fungus liquid.
Preferably, the pulling member includes an operating rod, the operating rod is slidably mounted in the sliding channel, one end of the operating rod is fixedly connected to the sealing plate, and the other end of the operating rod extends out of the sliding channel.
The operating rod is convenient for pulling the sliding plate, thereby being capable of pulling the sliding plate more conveniently.
Preferably, the number of the stirring rods is four, and the four stirring rods are uniformly and circumferentially distributed and fixedly arranged on the turntable.
The intestinal canal fungus liquid in the culture shell can be cultured more quickly by the four stirring rods.
Preferably, the bottom of the culture shell is fixedly provided with a mounting seat.
The mount pad is convenient for support cultivateing the casing.
The beneficial effect that this scheme produced is:
1. through the arrangement of the culture shell, the sealing mechanism, the rotary disc, the rotary shaft and the stirring rod, the intestinal bacteria liquid is cultured in the culture shell, and the sealing mechanism seals the intestinal bacteria liquid in the culture shell; rotate the rotation axis again, the rotation axis drives the carousel and rotates to drive the puddler through the carousel and rotate, thereby realize that the puddler carries out quick stirring to the intestinal fungus liquid of cultivateing in the culture shell, avoid the intestinal fungus liquid to receive the pollution.
2. Through the arrangement of the sealing plate, the spring, the push rod, the pull rod, the sliding plate and the pulling piece. The staff pulling pulls the piece, and the piece that pulls drives sliding plate and pull rod in proper order and slides together, and then makes the push rod remove, spring compression to make in the intestinal fungus liquid in the culture shell enters into the cavity through the inlet, the intestinal fungus liquid enters into the drain hole through the outage again, makes the intestinal fungus liquid discharge from the drain hole, thereby realizes carrying out quick sample to the intestinal fungus liquid in the culture shell.
Drawings
In order to more clearly illustrate the embodiments of the present invention, the drawings, which are required to be used in the embodiments, will be briefly described below. In all the drawings, the elements or parts are not necessarily drawn to actual scale.
FIG. 1 is a front view of an intestinal bacteria liquid culture device according to the present invention;
FIG. 2 is a right side view of an intestinal bacteria liquid culture apparatus according to the present invention;
FIG. 3 is a right side view of the intestinal bacteria liquid culture apparatus of the present invention at a turntable;
FIG. 4 is a right side view of the intestinal bacteria liquid culture apparatus of the present invention at the position of the stirring rod;
fig. 5 is a partially enlarged view of a portion a in fig. 1.
Reference numerals:
1-culture shell, 11-mounting seat, 12-cavity, 13-liquid inlet, 14-liquid outlet and 15-liquid outlet;
2-sealing mechanism, 21-sealing cover body, 22-sealing gasket and 23-carrying handle;
3-stirring mechanism, 31-rotating disc, 32-rotating shaft, 33-stirring rod, 34-motor and 35-belt;
4-sampling mechanism, 41-sealing plate, 42-spring, 43-push rod, 44-pull rod, 45-sliding plate, 46-pull piece, 461-operating rod, 47-sampling barrel and 48-communicating pipe.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
Referring to fig. 1-5, an intestinal bacteria liquid culture apparatus includes a culture housing 1, a sealing mechanism 2 and a stirring mechanism 3.
Wherein, cultivate the right side wall of casing 1 and be equipped with the mounting groove, the notch of mounting groove is equipped with the outside rotatory hole of intercommunication towards the inside of cultivateing casing 1, the left side wall of mounting groove. Specifically, the bottom of the culture shell 1 is fixedly provided with four mounting seats 11, and in the embodiment, the number of the mounting seats 11 is four. The four mounting seats 11 are installed on the ground, so that the culture shell 1 can be conveniently supported and fixed.
Wherein, the sealing mechanism 2 is hermetically sleeved on the culture shell 1. Specifically, the sealing mechanism 2 comprises a sealing cover body 21, a sealing gasket 22 and a carrying handle 23, the sealing cover body 21 is fitted on the top of the culture shell 1 through a threaded sealing cover, the sealing gasket 22 is fixedly installed at the bottom of the sealing cover body 21, the sealing gasket 22 can be inserted into an opening of the culture shell 1 in a vertically sliding sealing manner, the sealing gasket 22 can be sealed by an elastic rubber gasket, and the carrying handle 23 is fixedly installed at the top of the sealing cover body 21. The sealed lid body 21 lid closes and carries out preliminary sealed at the top of cultivateing casing 1, is carrying out sealed once more to cultivateing casing 1 through sealed 22 to further strengthened the sealed effect of cultivateing casing 1, mention carrying handle 23, thereby be convenient for remove sealed lid body 21 or cultivate casing 1.
Wherein, rabbling mechanism 3 includes carousel 31, rotation axis 32 and puddler 33, and carousel 31 rotates to be installed in the mounting groove, and rotatable seal installation in the rotation axis is downthehole for rotation axis 32, and the left end of rotation axis 32 stretches out the rotation axis, and the right-hand member portion fixed mounting of puddler 33 is on carousel 31, and another tip of puddler 33 extends to the one end of keeping away from rotation axis 32, and rotates with the lateral wall of cultivateing casing 1 and be connected to puddler 33 can rotate in the inside of cultivateing casing 1.
In order to stir more fully, four stirring rods 33 are provided, and four stirring rods 33 are uniformly and circumferentially distributed and fixedly arranged on the rotating disc 31. The four stirring rods 33 can culture the intestinal bacteria liquid in the culture shell 1 more quickly.
In this embodiment, referring to fig. 2, the stirring mechanism 3 further includes a motor 34 and a belt 35, the motor 34 is fixedly installed outside the left side of the culture enclosure 1, and the belt 35 is connected with the rotating shaft of the motor 34 and the rotating shaft 32 in a tensioning manner. Starting motor 34, motor 34 drive belt 35 and rotate, drive rotation axis 32 through belt 35 and rotate to make carousel 31 and puddler 33 rotate, stir intestinal fungus liquid through puddler 33.
When the culture device is used, the handle 23 is pressed, the sealing cover body 21 is rotated, the interior of the culture shell 1 is opened, a mixture for culturing intestinal bacteria liquid is added into the culture shell 1, and the culture shell 1 is sealed through the sealing cover body 21 and the sealing gasket 22, so that the mixture is cultured in the culture shell 1 in a sealing mode.
When needs stir the mixture of cultivateing intestinal fungus liquid, starter motor 34, motor 34 drives belt 35 and rotates, drive rotation axis 32 through belt 35 and rotate, so that rotation axis 32 drives carousel 31 and rotates, so as to drive puddler 33 through carousel 31 and rotate, make puddler 33 stir the intestinal fungus liquid of cultivateing in cultivateing casing 1, realize that the staff can be better stirs the cultivation fungus liquid under encapsulated situation, from avoiding the intestinal fungus liquid of cultivateing to receive the pollution.
In order to better sample the intestinal bacteria liquid in the culture shell 1, in this embodiment, please refer to fig. 1-5, and a sampling mechanism 4 is further included. The sampling mechanism 4 includes a sealing plate 41, a spring 42, a push rod 43, a pull rod 44, a sliding plate 45, a pulling member 46, a sampling barrel 47 and a communicating pipe 48.
Specifically, puddler 33 and carousel 31's inside all is equipped with the cavity 12 of mutual intercommunication, so that puddler 33's inside and carousel 31's inside communicates each other, the cavity 12 that is located puddler 33 inside is equipped with the inlet 13 that the casing 1 was cultivateed in the intercommunication, the cavity 12 circumference that is located carousel 31 inside is equipped with a plurality of outage 14, the axial of rotation axis 32 is equipped with and is located the slide channel of carousel 31 inside cavity 12 intercommunication, the bottom of mounting groove is equipped with the outside play liquid hole 15 of intercommunication, go out liquid hole 15 can communicate with outage 14.
The right side of the sealing plate 41 is hinged to the stirring rod 33, so that the sealing plate 41 can open or close the liquid inlet 13 in a sealing manner, the pull rod 44 is installed in the cavity 12 inside the stirring rod 33 in a left-right sliding manner, a gap exists between the pull rod 44 and the inner wall of the stirring rod 33, the left end of the pull rod 44 can extend into the cavity 12 inside the turntable 31, the top of the push rod 43 is hinged to the bottom of the left end of the sealing plate 41, the bottom of the push rod 43 is hinged to the pull rod 44, the spring 42 is sleeved on the pull rod 44, and two ends of the spring 42 are respectively fixedly connected with the surface of the pull rod 44 and the inside of the stirring rod 33.
The sliding plate 45 is sealingly mounted in the cavity 12 inside the turntable 31 to slide left and right along the axial direction of the rotating shaft, the rotating shaft is fixedly connected with the left end portion of the pull rod 44, the pulling member 46 is sealingly and slidably mounted in the sliding channel, one end portion of the pulling member 46 is fixedly connected with the sealing plate 41, the other end portion of the pulling member 46 extends out of the sliding channel, the spring 42 is in a natural state, the axial direction of the push rod 43 is perpendicular to the axial direction of the pull rod 44, the push rod 43 abuts against the sealing plate 41, so that the sealing plate 41 seals the liquid inlet 13, and the sliding plate 45 also seals the liquid outlet 14.
In this embodiment, referring to fig. 3, the pulling member 46 is an operating rod 461, the operating rod 461 is slidably mounted in the sliding channel, a right end of the operating rod 461 is fixedly connected to the sealing plate 41, and a left end of the operating rod 461 extends out of the sliding channel. The operating lever 461 facilitates pulling the sliding plate 45, thereby enabling the sliding plate 45 to be pulled more conveniently.
When the intestinal fungus liquid in the culture shell 1 needs to be sampled and detected, the operating rod 461 is pulled outwards, the operating rod 461 sequentially drives the sliding plate 45 and the pull rod 44 to slide, so that the push rod 43 moves leftwards, the spring 42 is compressed, the height of the top of the push rod 43 is reduced, and the left side of the sealing plate 41 is driven to move downwards, so that the intestinal fungus liquid in the culture shell 1 enters the cavity 12 through the liquid inlet 13, the liquid discharge hole 14 is opened through the sliding plate 45, the intestinal fungus liquid enters the liquid discharge hole 15 through the liquid discharge hole 14, the intestinal fungus liquid is discharged from the liquid discharge hole 15, so that the intestinal fungus liquid in the culture shell 1 can be sampled by a worker, and meanwhile, the intestinal fungus liquid in the intestinal culture device is also polluted.
According to the technical scheme, the culture shell 1, the sealing mechanism 2, the rotary disc 31, the rotary shaft 32 and the stirring rod 33 are arranged, so that the intestinal bacteria liquid is cultured in the culture shell 1, and the sealing mechanism 2 seals the intestinal bacteria liquid in the culture shell 1; the rotating shaft 32 is rotated again, the rotating shaft 32 drives the rotating disc 31 to rotate, so that the rotating disc 31 drives the stirring rod 33 to rotate, and the stirring rod 33 can rapidly stir the intestinal bacteria liquid cultured in the culture shell 1.
Through the arrangement of the sealing plate 41, the spring 42, the push rod 43, the pull rod 44, the sliding plate 45 and the pulling piece 46. The member 46 is drawn in the staff pulling, and the member 46 that draws drives sliding plate 45 and pull rod 44 in proper order and slides together, and then makes push rod 43 remove, the compression of spring 42 to make the intestinal fungus liquid of cultivateing in the casing 1 enter into cavity 12 through inlet 13 in, the intestinal fungus liquid enters into out liquid hole 15 through outage 14 again, make the intestinal fungus liquid follow out liquid hole 15 and discharge, thereby realize carrying out quick sample to the intestinal fungus liquid of cultivateing in the casing 1.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.

Claims (8)

1. The utility model provides an intestinal fungus liquid culture apparatus which characterized in that includes:
the culture shell is provided with a mounting groove on one side wall, and the mounting groove is provided with a rotating hole communicated with the outside;
the sealing mechanism is hermetically sleeved on the culture shell; and
rabbling mechanism, including carousel, rotation axis and puddler, the carousel rotate install in the mounting groove, the rotatable seal installation of rotation axis in the rotation hole, and keeping away from of rotation axis one end portion of carousel stretches out rotatory hole, a tip fixed mounting of puddler in on the carousel, another tip of puddler is to keeping away from the one end of rotation axis extends, and with the lateral wall of cultivateing the casing rotates and is connected.
2. The intestinal tract bacteria liquid culture device according to claim 1, wherein the sealing mechanism comprises a sealing cover body, a sealing gasket and a carrying handle, the sealing cover body covers the top of the culture shell, the sealing gasket is fixedly installed at the bottom of the sealing cover body, the sealing gasket is slidably and hermetically inserted into the opening of the culture shell, and the carrying handle is fixedly installed at the top of the sealing cover body.
3. The intestinal tract bacteria liquid culture device of claim 2, wherein the stirring mechanism further comprises a motor and a belt, the motor is fixedly installed outside the culture shell, and the belt is connected with a rotating shaft of the motor and the rotating shaft in a tensioning manner.
4. The intestinal tract bacteria liquid culture device of claim 3, further comprising a sampling mechanism, wherein the sampling mechanism comprises a sealing plate, a spring, a push rod, a pull rod, a sliding plate and a pulling piece;
cavities are formed in the stirring rod and the turntable, a liquid inlet communicated with the culture shell is formed in the cavity in the stirring rod, a plurality of liquid discharging holes are formed in the cavity in the turntable in the circumferential direction, a sliding channel communicated with the inside of the turntable is axially formed in the rotating shaft, a liquid discharging hole communicated with the outside is formed in the bottom of the mounting groove, and the liquid discharging hole can be communicated with the liquid discharging holes;
the sealing plate is hinged to the stirring rod and can open or close the liquid inlet in a sealing manner, the pull rod is slidably mounted inside the stirring rod, the top of the push rod is hinged to the bottom of the sealing plate, which is far away from the hinged end of the sealing plate, the bottom of the push rod is hinged to the pull rod, the spring is sleeved on the pull rod, and two ends of the spring are fixedly connected with the surface of the pull rod and the inside of the stirring rod respectively;
the sliding plate is arranged inside the rotary disc in a sealing mode along the axial direction of the rotating shaft in a sliding mode, the rotating shaft is fixedly connected with the end portion of the pull rod, the pulling piece is arranged in the sliding channel in a sliding mode, one end portion of the pulling piece is fixedly connected with the sealing plate, the other end portion of the pulling piece extends out of the sliding channel, the spring is in a natural state, the sealing plate seals the liquid inlet, and the sliding plate seals the liquid discharge hole.
5. The intestinal tract bacteria liquid culture device according to claim 4, wherein the sampling mechanism further comprises a sampling barrel and a communicating pipe, the sampling barrel is detachably mounted on the culture housing and is located at the bottom of the liquid outlet hole, and two ends of the communicating pipe are respectively in threaded sealing communication with the liquid outlet hole and the sampling barrel.
6. The intestinal tract bacteria liquid culture device of claim 5, wherein the pulling member comprises an operating rod, the operating rod is slidably installed in the sliding channel, one end of the operating rod is fixedly connected with the sealing plate, and the other end of the operating rod extends out of the sliding channel.
7. The intestinal tract bacteria liquid culture device of claim 6, wherein four stirring rods are provided, and four stirring rods are uniformly circumferentially distributed and fixedly mounted on the turntable.
8. The intestinal tract bacteria liquid culture device of claim 7, wherein a mounting seat is fixedly installed at the bottom of the culture shell.
CN202011459915.0A 2020-12-11 2020-12-11 Intestinal canal bacterium liquid culture apparatus Pending CN112646692A (en)

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Application publication date: 20210413