CN112617018B - Composition for resisting diseases and promoting growth and application thereof - Google Patents

Composition for resisting diseases and promoting growth and application thereof Download PDF

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CN112617018B
CN112617018B CN202011498621.9A CN202011498621A CN112617018B CN 112617018 B CN112617018 B CN 112617018B CN 202011498621 A CN202011498621 A CN 202011498621A CN 112617018 B CN112617018 B CN 112617018B
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parts
weight
tea tree
tree oil
butyrate
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CN112617018A (en
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肖丽萍
周盛昌
马尚威
苏建民
张敬学
丁能水
吴有林
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Jilin Yingshanhong Feed Co ltd
Liaoning Aonong Feed Co ltd
Sanming Aonong Biotechnology Co ltd
Fujian Aonong Biological Technology Group Co Ltd
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Jinhua Aonong Biological Science & Technology Co ltd
Liaoning Aonong Feed Co ltd
Sanming Aonong Biotechnology Co ltd
Fujian Aonong Biological Technology Group Co Ltd
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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    • A23V2400/00—Lactic or propionic acid bacteria
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Abstract

The invention discloses a disease-resistant growth-promoting composition, which is prepared from the following raw materials: fumaric acid, butyrate, probiotic powder, tea tree oil or modified tea tree oil. The disease-resistant growth-promoting composition disclosed by the invention plays a role in multiple dimensions of stress relief, enzyme secretion promotion, antibiosis and antiphlogosis and the like, enhances the immune function of animals, reduces the morbidity, improves the feed intake, daily gain and feed utilization rate of the animals, is natural, non-toxic and side-effect, free of drug resistance, suitable for resistance reduction and resistance replacement cultivation, and has a good application prospect. The probiotics, the butyrate and the tea tree oil are synergistic to resist bacteria and diminish inflammation, enhance the immunity, the anti-inflammatory function and the oxidation resistance, improve the disease resistance and reduce the morbidity.

Description

Composition for resisting diseases and promoting growth and application thereof
Technical Field
The invention relates to the technical field of feed additives, in particular to a disease-resistant growth-promoting composition and application thereof.
Background
In recent years, with the rapid development of intensive culture, the problems of diseases of animal intestinal tracts and the like are increased, and certain loss is caused to the breeding industry. The addition of antibiotics is an effective method for reducing animal diseases and improving breeding benefits. However, a large amount of antibiotics can destroy the ecological balance of beneficial flora in the animal body, so that the immunity of the animal is reduced, and the animal is easy to infect diseases; and long-term use of antibiotics can also enhance the drug resistance of bacteria, bring inflammation or toxic and side effects, cause the antibiotics to remain in animal meat products, pollute the environment and also cause serious harm to human health. Under the non-resistance breeding trend, a composition product capable of replacing antibiotics is developed and used for disease-resistant growth-promoting breeding of animals, and the composition product has great significance for sustainable development of breeding industry.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a composition for resisting diseases and promoting growth and application thereof.
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 2-5 parts of fumaric acid, 7-21 parts of butyrate, 1-2 parts of probiotic powder and 2-5 parts of tea tree oil.
Preferably, the disease-resistant and growth-promoting composition is prepared from the following raw materials in parts by weight: 2-5 parts of fumaric acid, 7-21 parts of butyrate, 1-2 parts of probiotic powder and 2-5 parts of modified tea tree oil.
The butyrate is at least one of calcium butyrate and coated sodium butyrate; preferably, the butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4 (1-3).
The probiotic powder is at least one of saccharomyces cerevisiae powder and lactobacillus plantarum powder; preferably, the probiotic bacteria powder is a mixture of saccharomyces cerevisiae bacteria powder and lactobacillus plantarum bacteria powder according to a mass ratio of (1-3) to 1.
Wherein, the fumaric acid can reduce the pH value of the gastrointestinal tract of the animal, promote the activity of digestive enzyme and improve the digestive function of the animal; the fumaric acid has stable property, can directly participate in tricarboxylic acid cycle to form ATP resynthesis somatic protein, can quickly supply energy under the stress or critical state of an organism, and is beneficial to improving the anti-stress capability and the digestion and absorption functions of animals; but also can improve the absorption and utilization rate of organisms on trace elements of calcium, magnesium and zinc under the synergistic action with butyrate, and is beneficial to improving daily gain, feed efficiency (reducing feed conversion ratio) and disease resistance (reducing diarrhea rate).
The effective component of the butyrate is butyric acid which is a main nutrient substance for regeneration and repair of intestinal epithelial tissue cells, and can quickly repair damaged intestinal mucosa and adjust the microecological balance of gastrointestinal tracts; can also stimulate the secretion of cystokinin in the pig body, enhance the activity of digestive enzyme, promote the digestion and absorption and improve the weight-increasing effect; meanwhile, the compound preparation can activate lymphocytes, promote the proliferation of intestinal mucosa immunocytes, strengthen the non-specific immunity of the intestinal tract and improve the disease resistance. Specifically, in the application, calcium butyrate mainly focuses on promoting the proliferation of beneficial bacteria and is cooperated with fumaric acid to improve the absorption and utilization rate of trace element calcium of an organism; the coated sodium butyrate can reduce the release of body cells Ca, has the effects of bacteriostasis and growth promotion under the synergistic action of fumaric acid, improves the intestinal microecological balance and effectively reduces the occurrence of diarrhea. Compared with sodium butyrate, the coated sodium butyrate has good fluidity and stability, realizes the slow release of the sodium butyrate in the digestive tract of animals, prevents the occurrence of nutritional diarrhea caused by colitis, and effectively avoids the influence of the sodium butyrate on the ingestion of the animals due to strong irritation, easy moisture absorption and odd odor; meanwhile, the flowability of the product can be improved through coating treatment.
As an excellent natural antibacterial agent, the tea tree oil has extremely strong antioxidation, has obvious killing and inhibiting effects on bacteria, fungi and viruses, has wide antibacterial spectrum and strong antibacterial activity, can improve the immunity of animals and the digestibility of nutrient substances, reduces the diarrhea rate, improves the production benefit and reduces the use of antibiotics.
The saccharomyces cerevisiae in the composite probiotics is facultative anaerobe, can consume oxygen in gastrointestinal tracts to cause an anaerobic environment, so that propagation of beneficial flora is promoted, microecological balance is improved, and the composite probiotics has physiological functions of immunoregulation, oxidation resistance and the like and can effectively adsorb pathogenic bacteria in intestinal tracts; the saccharomyces cerevisiae can convert metal elements into organic trace elements by extracellular enrichment, cell surface adsorption or complexation, intracellular enrichment and conversion and the like of the metal elements, and is favorable for improving the bioavailability; in addition, thalli are subjected to autolysis or catalytic hydrolysis by using exogenous enzyme to obtain yeast hydrolysate which is used as a supplement and a freshness-enhancing and flavor-developing agent of amino acid, polypeptide and B vitamins, so that the feed intake is promoted, important nutritional effects are played, and the secretion of digestive enzyme is promoted by cooperating with fumaric acid and sodium butyrate for coating, so that the digestion and absorption capacity is improved; the lactobacillus plantarum can produce a large amount of acid, and metabolites of the lactobacillus plantarum can adjust the acid environment in intestinal tracts, condition beneficial bacteria in the intestinal tracts and inhibit harmful bacteria, so that the feed digestion utilization rate and the health condition of swinery can be greatly improved, and the generation of harmful metabolites such as amine, ammonia, hydrogen sulfide, indole and the like in the intestinal tracts and the influence of the harmful metabolites on digestive tracts and even carcasses are reduced; in addition, the produced acidic substances can degrade heavy metals, reduce ammonia nitrogen emission and improve animal quality.
In the application of the invention, the saccharomyces cerevisiae has high fermentation speed, can decompose protein to generate various amino acids for beneficial bacteria, can promote the growth of the beneficial bacteria such as lactobacillus, can obviously improve the intestinal microecological area system when being used together with lactobacillus plantarum, can also cooperate with fumaric acid and butyrate to reduce the pH value of intestinal tracts, generates a large amount of digestive enzymes, promotes the digestion and absorption of nutrient components, improves the utilization rate of feed, enhances the immunity and disease resistance, and exerts the optimal effect of the symbiosis of the compound bacteria.
Because the tea tree oil is unstable, volatile, insoluble in water and poor in dispersibility, the active ingredients of the tea tree oil are easy to react in the presence of light or oxygen, and the tea tree oil also has pungent smell, the tea tree oil is further modified and optimized, so that the stability of the tea tree oil is improved, the smell can be covered, and the utilization efficiency and the efficacy of the tea tree oil are improved.
Preferably, the modified tea tree oil is prepared by the following method: preparing a modifier into a modifier aqueous solution with the mass concentration of 1.5-4.5wt%, adding tea tree oil drops into the modifier aqueous solution under the stirring condition of 100-300rpm, wherein the dropping speed is 2-5mL/min, and the mass ratio of the tea tree oil to the modifier is (3-5): 1; continuously stirring for 10-20min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; preferably, the spray drying conditions are: the air inlet temperature is 205-215 ℃, the needle is passed for 1-3 s/time, and the speed of the peristaltic pump is 20-60 rpm.
Preferably, the modifier is prepared by the following method: adding 3-6 parts by weight of pullulan into 95-115 parts by weight of PBS solution, stirring at 50-55 ℃ for 3-5min at the stirring speed of 100-; adding 1-3 parts by weight of ovalbumin into 95-105 parts by weight of PBS solution, stirring for 3-6min at the temperature of 30-40 ℃, and stirring at the speed of 100-300rpm to obtain an ovalbumin solution; adding 15-25 parts by weight of pullulan solution into 35-40 parts by weight of ovalbumin solution, uniformly mixing, and carrying out ultrasonic treatment at 60-65 ℃ for 30-40min, wherein the ultrasonic power is 400-600W and the ultrasonic frequency is 35-45 kHz; then heating to 80-90 ℃ and stirring for reaction for 35-45min, wherein the stirring speed is 100-300 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; preferably, the PBS solution is 0.01-0.03M PBS solution with pH of 5.5-6.5.
The modifier adopted by the invention has excellent dispersing performance and amphiphilic performance (both hydrophilic and oleophilic); wherein, the hydrophobic group exposed by the ovalbumin structure of the modifier can be oleophylically adsorbed with tea tree oil, thereby being well compatible to form stable emulsion; the pullulan structure of the modifier has a large number of hydrophilic groups, so that the hydrophilicity of the modified tea tree oil is improved, the dispersibility and stability of the tea tree oil in the feed are better, and the utilization efficiency is higher. In addition, the modified tea tree oil is coated inside, so that the special smell of the tea tree oil is covered, the feed intake is increased, and the utilization efficiency is further improved. The possible reasons are: in the preparation of the modifier, on one hand, pullulan is grafted to an ovalbumin structure through hydroxyl, so that the intermolecular steric hindrance is increased, a hydrophobic group in the protein is exposed outside, and the oleophylic adsorption performance of the modifier is improved; on the other hand, the ultrasonic and heating treatment is also beneficial to the development of protein, so that the exposure of an internal hydrophobic group is realized, and the oleophylic adsorption performance of the modifier is further improved; in addition, the pullulan grafted ovalbumin structure increases the steric hindrance and electrostatic repulsion between molecules, so that the pullulan grafted ovalbumin is not easy to agglomerate and has better dispersibility.
Further preferably, the modifier is prepared by the following method: adding 3-6 parts by weight of pullulan into 95-115 parts by weight of PBS solution, stirring at 50-55 ℃ for 3-5min at the stirring speed of 100-; adding 3-6 parts by weight of dextran into 95-115 parts by weight of PBS solution, stirring at 50-55 ℃ for 3-5min at the stirring speed of 100-300rpm to obtain dextran solution; adding 1-3 parts by weight of ovalbumin into 95-105 parts by weight of PBS solution, stirring for 3-6min at the temperature of 30-40 ℃, and stirring at the speed of 100-300rpm to obtain an ovalbumin solution; sequentially adding 3-7 parts by weight of dextran solution and 12-18 parts by weight of pullulan solution into 35-40 parts by weight of ovalbumin solution, uniformly mixing, and carrying out ultrasonic treatment at 60-65 ℃ for 30-40min with the ultrasonic power of 400-600W and the ultrasonic frequency of 35-45 kHz; then the temperature is increased to 80-90 ℃ and the stirring reaction is carried out for 35-45min, the stirring speed is 100-300 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; preferably, the PBS solution is 0.01-0.03M PBS solution with pH of 5.5-6.5.
The invention also discloses application of the disease-resistant growth-promoting composition in animal feeding.
Preferably, the disease-resistant and growth-promoting composition is added into animal feed in an amount of 0.5-2 wt%.
The invention has the beneficial effects that: the disease-resistant growth-promoting composition disclosed by the invention plays a role in multiple dimensions of stress relief, enzyme secretion promotion, antibiosis and antiphlogosis and the like, enhances the immune function of animals, reduces the morbidity, improves the feed intake, daily gain and feed utilization rate of the animals, is natural, non-toxic and side-effect, free of drug resistance, suitable for resistance reduction and resistance replacement cultivation, and has a good application prospect. The fumaric acid can reduce the pH of the gastrointestinal tract, relieve external stress and reduce intestinal injury, and can improve the absorption and utilization rate of organisms on trace elements of calcium, magnesium and zinc by cooperating with the butyrate, thereby being beneficial to improving daily gain, feed efficiency and disease resistance; the fumaric acid, the butyrate and the composite probiotics can promote the secretion of digestive enzyme, enhance the digestion and absorption capacity and improve the weight gain effect through the mutual synergistic effect. On the other hand, the composite probiotics, the butyrate and the tea tree oil are synergistic to resist bacteria and diminish inflammation, enhance the immunity, anti-inflammatory function and oxidation resistance, improve the disease resistance and reduce the morbidity; the composite probiotics can promote the reproduction of beneficial bacteria, inhibit the growth of harmful bacteria and even effectively adsorb pathogenic bacteria in intestinal tracts, and simultaneously has physiological functions of immunoregulation, oxidation resistance and the like; the butyrate has obvious bacteriostatic and growth promoting effects, improves the intestinal microecological balance, can also activate lymphocytes, promotes the proliferation of intestinal mucosa immunocytes and improves the disease resistance; the tea tree oil is an excellent natural antibacterial agent, has antibacterial and bacteriostatic effects and has strong antioxidation effect. The composite probiotics, butyrate and tea tree oil in the disease-resistant growth-promoting composition provided by the invention are synergistic, so that the multi-dimensional disease-resistant growth-promoting composition can relieve stress, promote enzyme secretion, resist bacteria, diminish inflammation and the like, and can effectively replace antibiotics to be used for animal feeding. In addition, the disease-resistant growth-promoting composition is also beneficial to improving the lean meat percentage of the fattening pigs.
Detailed Description
The above summary of the present invention is described in further detail below with reference to specific embodiments, but it should not be understood that the scope of the above subject matter of the present invention is limited to the following examples.
Introduction of some raw materials in this application:
fumaric acid, CAS: 110-17-8; calcium butyrate, CAS: 5743-36-2; tea tree oil, CAS: 68647-73-4.
The sodium butyrate is coated and chemically coated by sodium butyrate (CAS: 156-54-7) through an intelligent microcapsule technology, the content of the sodium butyrate is 30 percent, and the sodium butyrate is purchased from Jia Zhi Nuo biological science and technology limited company in Foshan city.
The effective viable bacteria content of Saccharomyces cerevisiae powder is not less than 1 × 108CFU/g, the number of the saccharomyces cerevisiae is CICC 31481; the effective viable bacteria content of the lactobacillus plantarum bacterial powder is more than or equal to 1 multiplied by 1011CFU/g, the number of the lactobacillus plantarum is CGMCC 7928.
The specific techniques or conditions not mentioned in the examples are performed according to the techniques or conditions conventional in the art or according to the product specification. Reagents or instruments of manufacturers are not indicated, and all the conventional products are commercially available.
Comparative example 1
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 1.6 parts of probiotic powder and 3 parts of tea tree oil.
The probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
Comparative example 2
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate and 1.6 parts of probiotic powder.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
Comparative example 3
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate and 3 parts of tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3.
Example 1
The composition for resisting diseases and promoting growth consists of the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
Example 2
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 2 parts of fumaric acid, 7 parts of butyrate, 1 part of probiotic powder and 2 parts of tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 2; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 2: 1.
Example 3
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 5 parts of fumaric acid, 21 parts of butyrate, 2 parts of probiotic powder and 5 parts of tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 1; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder in a mass ratio of 1: 1.
Test example 1
The test uses a single factor design, and 210 healthy growing pigs (piglets) with health status and weight close to that of the external ternary 'Duzhao' and weight of 11.5 +/-0.5 kg are selected and randomly divided into 7 groups (5 replicates for each group, 6 piglets for each replicate), wherein 1 group of control groups (without the addition of the disease-resistant and growth-promoting composition) and 6 groups of test groups (with the addition of 1 wt% of the disease-resistant and growth-promoting composition according to comparative examples 1-3 and examples 1-3, respectively) are each matched with a basic ration (with reference to nutritional needs of NRC (2012) pigs in the United states). And in the pre-feeding period of 7 days, feeding the pigs for 30 days by using the daily ration of a control group and the daily ration of a test group respectively, performing feeding management programs and immunization programs in the test period according to a conventional commercial pig feeding management manual, recording the feed consumption in the test period, and weighing. After the test is finished, the production performance index of the piglet is measured, and the result is shown in table 1; from each group, 3 piglets were randomly selected and sacrificed by bleeding from the jugular vein, the abdominal cavity was opened immediately after sacrifice, the contents in the anterior jejunum and the posterior jejunum were collected under aseptic conditions and stored in liquid nitrogen, and the activities of protease, amylase and lipase in the jejunum contents were measured using a kit (provided by Nanjing institute of bioengineering), and the results are shown in Table 2.
(1) The daily ration of the control group consists of the following raw materials: 70.6 wt% of corn, 18 wt% of soybean meal (46.5%), 1 wt% of stone powder, 1 wt% of calcium hydrophosphate, 0.4 wt% of salt, 3 wt% of premix and 6 wt% of wheat bran; namely, the composition for resisting diseases and promoting growth is not added.
(2) The test group daily ration comprises the following raw materials: 70.6 wt% of corn, 18 wt% of soybean meal (46.5%), 1 wt% of stone powder, 1 wt% of calcium hydrophosphate, 0.4 wt% of salt, 3 wt% of premix, 5wt% of wheat bran and 1 wt% of disease-resistant and growth-promoting composition; namely, the disease-resistant and growth-promoting compositions of comparative examples 1 to 3 and examples 1 to 3 were added, respectively.
Wherein, the soybean meal (46.5%) refers to soybean meal with protein content of 46.5 wt%; the premix is prepared from commercially available pig premix, and provides VA 5000IU, VD 1500IU, VE 12mg and VK for one kilogram of daily ration3 2mg、VB1 1mg、VB2
3mg、VB6 2mg、VB1212 mu g, 14mg nicotinic acid, 0.4mg folic acid, 7mg pantothenic acid, 350mg choline, 100mg iron, 100mg zinc, 30mg manganese, 10mg copper, 0.3mg selenium and 0.5mg iodine.
TABLE 1 comparison of the growth Performance of the disease-resistant growth-promoting compositions on piglets
Figure BDA0002842941100000091
As can be seen from table 1, compared with the control group without the addition of the disease-resistant growth-promoting composition, the addition of the disease-resistant growth-promoting composition prepared in examples 1 to 3 of the present invention to the daily ration can effectively improve the growth performance of piglets, wherein the diarrhea rate is not higher than 10%, and is obviously lower than the diarrhea rate of 33.33% in the control group, which indicates that the disease-resistant growth-promoting composition prepared in examples 1 to 3 of the present invention has a good effect of reducing diarrhea of piglets. By feeding the disease-resistant growth-promoting composition, the feed intake, daily gain and feed utilization rate of animals can be improved, digestion and absorption are promoted, the growth performance is improved, the intestinal function is improved, and the diarrhea rate is reduced.
TABLE 2 comparison of the disease-resistant growth-promoting compositions for the digestive enzyme activities of the anterior and posterior jejunum segments of piglets
Figure BDA0002842941100000092
Figure BDA0002842941100000101
Note: a. b indicates that there is a significant difference in the test results (P < 0.05).
As can be seen from table 2, compared with the control group without the addition of the disease-resistant growth-promoting composition, the addition of the disease-resistant growth-promoting composition prepared in the embodiments 1 to 3 of the present invention to the ration can effectively improve the intestinal digestive enzyme activity of piglets, wherein the amylase and protease activities of the jejunum forepart are significantly improved (P < 0.05); the activities of amylase, lipase and protease at the rear segment of jejunum are also obviously improved (P is less than 0.05), which shows that the activity of digestive enzyme of intestinal tracts of piglets can be improved, the digestive absorption capacity is enhanced and the growth is promoted by feeding the disease-resistant growth-promoting composition.
Example 4
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modified tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae bacteria powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modified tea tree oil is prepared by the following method: preparing a modifier into a modifier aqueous solution with the mass concentration of 3.2 wt%, adding tea tree oil drops into the modifier aqueous solution under the stirring condition of 200rpm, wherein the dropping speed is 3mL/min, and the mass ratio of the tea tree oil to the modifier is 4.5: 1; continuously stirring for 15min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; the conditions of the spray drying are as follows: the air inlet temperature is 210 ℃, the needle is communicated for 2 s/time, and the peristaltic pump is 55 rpm.
The modifier is prepared by the following method: adding 5 parts by weight of pullulan into 100 parts by weight of PBS solution, stirring at 52 ℃ for 3.5min and at the stirring speed of 200rpm to obtain a pullulan solution; adding 2 parts by weight of ovalbumin into 100 parts by weight of PBS solution, stirring for 5min at 35 ℃, and stirring at the speed of 200rpm to obtain an ovalbumin solution; adding 21 parts by weight of pullulan solution into 38 parts by weight of egg white protein solution, uniformly mixing, and carrying out ultrasonic treatment at 63 ℃ for 35min with the ultrasonic power of 500W and the ultrasonic frequency of 40 kHz; then heating to 85 ℃, stirring and reacting for 40min, wherein the stirring speed is 200 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; the PBS solution was 0.02M, pH6.0 PBS solution. Wherein, pullulan, CAS: 9057-02-7 and a viscosity of 180mpa.s, available from Dalian Meiren Biotechnology Ltd. Ovalbumin, CAS: 9006-59-1, MW 45000, from hen eggs, available from Bailingwei technologies, Inc., Beijing.
Example 5
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modified tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modified tea tree oil is prepared by the following method: preparing a modifier into a modifier aqueous solution with the mass concentration of 3.2 wt%, adding tea tree oil drops into the modifier aqueous solution under the stirring condition of 200rpm, wherein the dropping speed is 3mL/min, and the mass ratio of the tea tree oil to the modifier is 4.5: 1; continuously stirring for 15min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; the conditions of the spray drying are as follows: the air inlet temperature is 210 ℃, the needle is communicated for 2 s/time, and the peristaltic pump is 55 rpm.
The modifier is prepared by the following method: adding 5 parts by weight of pullulan into 100 parts by weight of PBS solution, stirring at 52 ℃ for 3.5min and at the stirring speed of 200rpm to obtain a pullulan solution; adding 5 parts by weight of dextran into 100 parts by weight of PBS solution, stirring at 52 ℃ for 3.5min and at a stirring speed of 200rpm to obtain dextran solution; adding 2 parts by weight of ovalbumin into 100 parts by weight of PBS solution, stirring for 5min at 35 ℃, and stirring at the speed of 200rpm to obtain an ovalbumin solution; sequentially adding 6 parts by weight of glucan solution and 15 parts by weight of pullulan solution into 38 parts by weight of ovalbumin solution, uniformly mixing, and carrying out ultrasonic treatment at 63 ℃ for 35min with the ultrasonic power of 500W and the ultrasonic frequency of 40 kHz; then heating to 85 ℃, stirring and reacting for 40min, wherein the stirring speed is 200 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; the PBS solution was 0.02M, pH6.0 PBS solution. Wherein, pullulan, CAS: 9057-02-7 and a viscosity of 180mpa.s, available from Dalian Meiren Biotechnology Ltd. Dextran, i.e. dextran T-3, CAS: 9004-54-0, molecular weight 3000, available from Hefeibomei Biotech, Inc. Ovalbumin, CAS: 9006-59-1, MW 45000, from hen eggs, available from Bailingwei technologies, Inc., Beijing.
Comparative example 4
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modified tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modified tea tree oil is prepared by the following method: preparing a modifier into a modifier aqueous solution with the mass concentration of 3.2 wt%, adding tea tree oil drops into the modifier aqueous solution under the stirring condition of 200rpm, wherein the dropping speed is 3mL/min, and the mass ratio of the tea tree oil to the modifier is 4.5: 1; continuously stirring for 15min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; the conditions of the spray drying are as follows: the air inlet temperature is 210 ℃, the needle is communicated for 2 s/time, and the peristaltic pump is 55 rpm.
The modifier is prepared by the following method: adding 5 parts by weight of dextran into 100 parts by weight of PBS solution, stirring at 52 ℃ for 3.5min and at a stirring speed of 200rpm to obtain dextran solution; adding 2 parts by weight of ovalbumin into 100 parts by weight of PBS solution, stirring for 5min at 35 ℃, and stirring at the speed of 200rpm to obtain an ovalbumin solution; adding 21 parts by weight of dextran solution into 38 parts by weight of ovalbumin solution, uniformly mixing, and carrying out ultrasonic treatment at 63 ℃ for 35min with ultrasonic power of 500W and ultrasonic frequency of 40 kHz; then heating to 85 ℃, stirring and reacting for 40min, wherein the stirring speed is 200 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; the PBS solution was 0.02M, pH6.0 PBS solution. Wherein dextran, i.e. dextran T-3, CAS: 9004-54-0, molecular weight 3000, available from Hefeibomei Biotech, Inc. Ovalbumin, CAS: 9006-59-1, MW 45000, from hen eggs, available from Bailingwei technologies, Inc., Beijing.
Comparative example 5
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modified tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modified tea tree oil is prepared by the following method: preparing pullulan into a pullulan water solution with the mass concentration of 3.2 wt%, adding tea tree oil drops into the pullulan water solution under the stirring condition of 200rpm, wherein the dropping speed is 3mL/min, and the mass ratio of the tea tree oil to the pullulan is 4.5: 1; continuously stirring for 15min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; the conditions of the spray drying are as follows: the air inlet temperature is 210 ℃, the needle is communicated for 2 s/time, and the peristaltic pump is 55 rpm. Wherein, pullulan, CAS: 9057-02-7 and a viscosity of 180mpa.s, available from Dalian Meiren Biotechnology Ltd.
Comparative example 6
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modified tea tree oil.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae bacteria powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modified tea tree oil is prepared by the following method: preparing ovalbumin into an ovalbumin aqueous solution with the mass concentration of 3.2 wt%, adding tea tree oil drops into the ovalbumin aqueous solution under the stirring condition of 200rpm, wherein the dropping speed is 3mL/min, and the mass ratio of the tea tree oil to the ovalbumin is 4.5: 1; continuously stirring for 15min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil; the conditions of the spray drying are as follows: the air inlet temperature is 210 ℃, the needle is communicated for 2 s/time, and the peristaltic pump is 55 rpm. Wherein, ovalbumin, CAS: 9006-59-1, MW 45000, from hen eggs, available from Bailingwei technologies, Inc., Beijing.
Comparative example 7
The composition for resisting diseases and promoting growth is prepared from the following raw materials in parts by weight: 3 parts of fumaric acid, 14 parts of butyrate, 1.6 parts of probiotic powder and 3 parts of modifier.
The butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4: 3; the probiotics is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder according to a mass ratio of 3: 1.
The modifier is prepared by the following method: adding 5 parts by weight of pullulan into 100 parts by weight of PBS solution, stirring at 52 ℃ for 3.5min and at the stirring speed of 200rpm to obtain a pullulan solution; adding 2 parts by weight of ovalbumin into 100 parts by weight of PBS solution, stirring for 5min at 35 ℃, and stirring at the speed of 200rpm to obtain an ovalbumin solution; adding 21 parts by weight of pullulan solution into 38 parts by weight of egg white protein solution, uniformly mixing, and carrying out ultrasonic treatment at 63 ℃ for 35min with the ultrasonic power of 500W and the ultrasonic frequency of 40 kHz; then heating to 85 ℃, stirring and reacting for 40min, wherein the stirring speed is 200 rpm; cooling to room temperature, and freeze-drying to obtain a modifier; the PBS solution was 0.02M, pH6.0 PBS solution. Wherein, pullulan, CAS: 9057-02-7 and a viscosity of 180mpa.s, available from Dalian Meiren Biotechnology Ltd. Ovalbumin, CAS: 9006-59-1, MW 45000, from hen eggs, available from Bailingwei technologies, Beijing.
Test example 2
400 tripartite crossbred fertile pigs with the weight of 83 +/-0.21 kg are selected and randomly divided into 8 groups, each group has 10 repetitions, and each repetition has 5 repetitions. The antibiotic group is a basic ration to which 0.05 wt% of chlortetracycline (with a purity of 15%) is added, and the test group is a basic ration to which 0.5 wt% of the disease-resistant and growth-promoting composition of the examples and the comparative examples is added, respectively, for a test period of 40 days. During the test period, the patients can eat and drink water freely. Before feeding every morning, the records are observed, the pigsty is cleaned every day, and the feeding management and the environmental conditions of each group are consistent. According to the technical specification for measuring the performance of lean type pigs, NY/T62-1987, the eye muscle area (cm) of the fattening pigs is measured2): the contour of the eye muscle is drawn on the parchment paper by a pencil, and the area of the eye muscle is calculated by an integrator. The average daily food consumption means the average daily food consumption of each pig during the test period, i.e. the average daily food consumption is (weight of feed-weight remaining after feeding)/(number of days × number of heads).
The formula of the basic ration is as follows: 71.2 wt% of corn (grade 1, 8.7 wt% ]), 19 wt% of soybean meal (grade 2, 44.2 wt% ], 1 wt% of stone powder, 0.4 wt% of calcium hydrogen phosphate, 0.3 wt% of salt, 1 wt% of premix, 0.1 wt% of L-lysine hydrochloride, and 7 wt% of wheat bran (grade 2, 14.3 wt% ]). Wherein, the premix comprises the following components: vitamin A13750 IU, vitamin D33750 IU, vitamin E70 mg, vitamin K37.5mg, vitamin B1
6.25mg, vitamin B216.75mg, vitamin B614.375mg, vitamin B120.075mg, biotin 0.1mg, folic acid 2.75mg, nicotinic acid 88.75mg, D-calcium pantothenate 30mg, ferrous sulfate 407.5mg, zinc oxide 180mg, manganese oxide 105mg, copper sulfate 23.75mg, sodium selenite 4mg, and potassium iodide 1.125 mg.
TABLE 3 Effect of disease-resistant growth-promoting compositions on growing finishing pigs
Figure BDA0002842941100000161
As can be seen from Table 3, after the disease-resistant growth-promoting composition is fed, the growing-finishing pigs are active and good in feeding, the eye muscle area of the growing-finishing pigs is increased, and the disease-resistant growth-promoting composition can effectively improve the average daily feed intake, promote the growth and improve the lean meat percentage of the growing-finishing pigs.
The embodiments described above are intended to facilitate the understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.

Claims (2)

1. The composition for resisting diseases and promoting growth is characterized by comprising the following raw materials in parts by weight: 2-5 parts of fumaric acid, 7-21 parts of butyrate, 1-2 parts of probiotic powder and 2-5 parts of modified tea tree oil;
the butyrate is a mixture of calcium butyrate and coated sodium butyrate according to a mass ratio of 4 (1-3);
the probiotic powder is a mixture of saccharomyces cerevisiae powder and lactobacillus plantarum powder in a mass ratio of (1-3) to 1;
the modified tea tree oil is prepared by the following method: preparing a modifier into a modifier aqueous solution with the mass concentration of 1.5-4.5wt%, adding tea tree oil drops into the modifier aqueous solution under the stirring condition of 100-300rpm, wherein the dropping speed is 2-5mL/min, and the mass ratio of the tea tree oil to the modifier is (3-5): 1; continuously stirring for 10-20min after the dropwise adding is finished, and performing spray drying to obtain modified tea tree oil;
the modifier is prepared by the following method: adding 3-6 parts by weight of pullulan into 95-115 parts by weight of PBS solution, stirring at 50-55 ℃ for 3-5min at the stirring speed of 100-; adding 1-3 parts by weight of ovalbumin into 95-105 parts by weight of PBS solution, stirring for 3-6min at the temperature of 30-40 ℃, and stirring at the speed of 100-300rpm to obtain an ovalbumin solution; adding 15-25 parts by weight of pullulan solution into 35-40 parts by weight of ovalbumin solution, uniformly mixing, and carrying out ultrasonic treatment at 60-65 ℃ for 30-40min, wherein the ultrasonic power is 400-600W and the ultrasonic frequency is 35-45 kHz; then heating to 80-90 ℃ and stirring for reaction for 35-45min, wherein the stirring speed is 100-300 rpm; cooling to room temperature, and freeze-drying to obtain the modifier.
2. Use of the disease-resistant, growth-promoting composition of claim 1 in the preparation of animal feed.
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